CN102010830A - Spirulina acclimatization method and application thereof - Google Patents
Spirulina acclimatization method and application thereof Download PDFInfo
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Abstract
The invention discloses a spirulina acclimatization method and application thereof. The method comprises of preparing a culture medium, carrying out salt resistance acclimatization, nitrogen and phosphorus content reduction acclimatization, to-be-treated water quality adaptation acclimatization and the like on screened algae. After the step-by-step amplification culture, the algae is introduced into kaline soil, the spirulina propagate massively by using the photosynthesis and absorbs and fixs nitrogen and phosphorus elements in the atmosphere and water sources, after the propagation, spirulina culture water is discharged, the spirulina is deposited in the soil, and the spirulina contains a large amount of elements such as nitrogen, phosphorus and the like. When the primary treatment is carried out by adopting the method, the nitrogen content in the soil is increased by 21 to 23 percent, the phosphorus content in the soil is increased by 11 to 14 percent, the total salt content is reduced by 20 to 25 percent, the nutrition element loss caused by a water washing method is avoided, the hardening on the soil and the secondary pollution to water sources and the environment caused by fertilizer application are also avoided, the soil fertility is gradually enhanced through performing the treatment many times, the soil recovery can be accelerated, and the goal of improving the ecological environment is finally achieved.
Description
Technical field
The invention discloses a kind of method of utilizing fixed nitrogen blue-green algae improvement saltings, increasing soil fertility, particularly a kind of method of utilizing spirulina to handle the saltings.
Background technology
The saltings distributes very wide all over the world, spread all over more than 30 countries in six continents.Mainly be distributed in countries such as India, Pakistan, Russia, South Africa, about 9.56 hundred million hectares of the total area.Wherein India and Pakistan are two state-owned 8,090,000 hectares, 7,500,000 hectares of Russia.The saltings area of Western United States accounts for about half of irrigation district area, and reduce by 25% or more because of saline and alkaline output every year.The soil salinization is the important component part that constitutes desertification of land, and local husbandry production in serious threat, the solution of a series of significant problems of improvement of the development and prosperity of national economy and ecotope.For a long time, scientist has carried out many-sided exploration and research to the improvement in saltings both at home and abroad.In 20 beginnings of the century characteristic is learned in regional distribution, formation reason, evolution process and the generation in saltings and carried out many-sided research.Set up the thirties with hydro project, soil improvement is the anti-salt engineering system (digging canal, open drain, hidden pipe, well-digging water lift etc.) of irrigation, water quality, antiseepage and irrigation and drainage at center.Over nearly 40 years, some economic flourishing countries of USSR (Union of Soviet Socialist Republics), the U.S. are with physics, chemistry and agricultural measures improvement saltings, as use industry byproduct (slag, gypsum) and make soil improvement agent, and it is saline and alkaline to join flushing with clean water, to improve soil physico-chemical property.But these measures need bigger material resources, and financial resources and a large amount of fresh water also are difficult to widespread use.The fifties in last century USSR (Union of Soviet Socialist Republics) just filter out some saline alkali tolerant plants, as alkali wheatgrass, Itanlian rye, cogongrass, river grain uncle clover etc.The U.S. once selected salt-tolerant plants such as shore rattan, heavy foliage shore rattan, still was in conceptual phase at present, was not applied to as yet produce.China has all kinds ofly is rich in saline and alkaline land area and has 3,460 ten thousand square kilometres approximately, mainly be distributed in Xinjiang, Gansu, Ningxia, the Qinghai of song-Nen plain, the Yellow River and Huai He River Haiti district, the Northwest, the Inner Mongol, Shanxi, Hebei and the three provinces in the northeast of China in North China, the saltings that the whole nation has 17 provinces (district) to have area not wait also has 80% left and right sides saline soil not obtain development and use as yet up to now.The alkaline land soil structure is viscid, and air permeability is poor, and the aerobic bacteria reactivity is poor in the soil.The saltings is divided into following three kinds by salinity: slight salinization soil, soil salt content 0.1-0.2%; Moderate salinization soil, soil salt content 0.2-0.4%; Severe salinization soil, soil salt content 0.4-0.6%; (China's great majority calculate saltiness with 30 centimetres of soil plough horizons.) in the main method in existing improvement saltings, the washing salt discharge that physical method adopts causes the disappearance of soil nutrient composition easily, and need bigger material resources and financial resources, and fertilising or Balancing that chemical process adopts cause soil compaction and too high control expense easily.Though the saline and alkaline that the method for physics and chemistry can temporary change soil can not be from changing the soil fertility in original saltings in essence.
Utilize biological modification, particularly spirulina technique improvement saltings can increase loose soil property, increases soil permeability, increases soil fertility, repairs problem thereby thoroughly solve salinized soil.
Summary of the invention
One of the object of the invention provides a kind of substratum of culturing spirulina.
Two of the object of the invention provides a kind of acclimation method and application thereof of spirulina, comprises the method in the spirulina processing saltings of using after taming.
As everyone knows, contain 80% the nitrogen of having an appointment in the air, but plant, animal, the mankind and most of microbe all can not directly utilize this gaseous nitrogen as nitrogen nutrition, occurring in nature has only few a part of microorganism nitrogen progressively can be reduced to ammonia and as nitrogenous source, and will be more than by industrial fixed nitrogen amount height by biological nitrogen fixation fixed nitrogen amount.Blue-green algae in the world has 2000 kinds approximately, and wherein the fixed nitrogen blue-green algae is unique biology that can utilize carbonic acid gas and nomadic nitrogen in the photosynthesis fixed air under aerobic conditions.In the fixed nitrogen blue-green algae, spirulina (Spirulina pla tensis) extensively is distributed in each sea area, the world and land is light, in the salina.Spirulina contains essential nutritive ingredient of a large amount of animals and physiologically active substance, Phycocyanins, C-in the spiral frond and spirulina polysaccharide have the physiologically active of adjusting and activate immunity reaction, contain a large amount of gamma-linolenic acids the cardiovascular and cerebrovascular normal function of safeguarding humans and animals is played special role, spirulina is defined as " optimum medicines of human 21 century " by The World Health Organization (WHO).Spirulina is that spiral is thread in its form of microscopically, the well-regulated helix-coil of algal filament, vivid blue green or yellow.Be fit to be grown in static or the mobile water body, on the slowly drained soil, mix to give birth to or in other algae in the salt lake.Spirulina has certain tolerance of salinity generally speaking, when salinity is 1.5g/L, the photosynthetic oxygen evolution activity is higher, and the photosynthetic oxygen activity of putting obviously descends when salinity reaches 5.0g/L, have only about 45% of normal value, and the present invention is with after the spirulina domestication, makes it still can the high-speed rapid growth breeding in the saltings, and the nitrogen, the phosphorus that absorb in a large number in airborne nitrogen and the water is fixed in the frond, finally stays in the saltings.Because the optimal growth temperature of spirulina is 25～30 ℃, temperature be lower than 15 ℃ or be higher than 40 ℃ all unfavorable to growing, the pH value is 8.5～10, and suitable intensity of illumination is 10000～30000 luxs, is fit to handle higher geographic moderate of the coastal temperature of China or heavy salinized ground.
The additive that is mainly used in healthcare products and food or feed of spirulina in the prior art, and the inventive method is by preparing specific substratum, the spirulina algae kind that filters out is carried out tolerance of salinity domestication, reduce the nitrogen and phosphorus content domestication and adapt to domestication such as pending water quality, through after the amplification culture step by step, the algae kind is introduced in the saltings, spirulina utilizes photosynthesis to breed in a large number, and can absorb, the fixing nitrogen element in atmosphere and the water source, absorb the phosphoric in the water source, after breed after a while, spirulina breeding water is drained, the spiral shell spirulina is deposited in the soil gradually, owing to accumulated a large amount of nutritive elements such as nitrogen phosphorus in the spiral frond, and after the septic spirulina degraded, can improve the nitrogen and phosphorus content in the saltings greatly, the moisture of culturing spirulina can be with saline and alkaline the taking away in the soil, and the spirulina improved treatment through repeated multiple times can make soil fertility be enhanced gradually, and can fundamentally solve the problem of salting of soil, finally reach the purpose of improving the ecological environment.
The spirulina improvement saltings that utilizes of the present invention, the method for increase alkaline land soil fertility, realize by following steps:
Above-mentioned purpose of the present invention, realize by following steps:
Step 1: screening spirulina algae kind, and at the algae kind preparation substratum that filters out;
(1) condition of screening algae kind
Algae kind of the present invention is collected in area, Beijing-Tianjin Ji, North China, after separation and purification voluntarily, chooses well-grown, stabilization characteristics of genetics, and growth cycle is short, the biomass height of accumulation, culture condition is wide in range, and the spirulina with fixed nitrogen characteristic is preferred algae kind;
(2) the FN32 substratum (basic medium) of spirulina is cultured in preparation:
Sodium bicarbonate 5-35g/L sodium-chlor 0.5-3.0g/L
Yellow soda ash 1-9g/L sal epsom 0.05-0.2g/L
Dipotassium hydrogen phosphate 0.1-1.8g/L calcium chloride 0.01-0.09g/L
SODIUMNITRATE 0.8-4.0g/L Plant hormones regulators,gibberellins 0.05-0.4mg/L
Trace element 0.5-2ml/L
Wherein Wei Liangyuansu prescription is:
Boric acid 1-5mg iron(ic) chloride 150-800mg
Zinc sulfate 0.04-0.3mg Sodium orthomolybdate 5-40mg
Manganous sulfate 0.35-2.5mg cobalt chloride 3.5-10mg
Copper sulfate 0.02-0.09mg adding distil water is settled to 1L
(3) be 25-30 ℃ in temperature, illumination is the 10000-30000 lux, and pH under 8.5～10 the condition, cultivated 5-8 days.
Culture medium prescription is preferably:
Sodium bicarbonate 10-15g/L sodium-chlor 1.0-2.0g/L
Yellow soda ash 2.5-5g/L sal epsom 0.05-0.15g/L
Dipotassium hydrogen phosphate 0.3-0.6g/L calcium chloride 0.02-0.05g/L
SODIUMNITRATE 1-3.5g/L Plant hormones regulators,gibberellins 0.1-0.3mg/L
Trace element 0.5-1.5ml/L
Step 2: the purposes by the saltings is carried out the adaptability domestication to spirulina:
(1). tolerance of salinity domestication: at the situation on moderate or heavy salinized ground, increase sodium chloride content in the basic medium gradually, spirulina is adapted to the cultivation of saline and alkaline, make it can adapt to more and more higher salinity gradually, and still can keep the high-speed rapid growth breeding, its tolerance of salinity domestication culture medium prescription is:
Sodium bicarbonate 5-35g/L sal epsom 0.05-0.2g/L
Yellow soda ash 1-9g/L calcium chloride 0.01-0.09g/L
Dipotassium hydrogen phosphate 0.1-1.8g/L Plant hormones regulators,gibberellins 0.1-0.3mg/L
SODIUMNITRATE 0.8-4.0g/L trace element 0.5-2ml
Sodium-chlor is increased to 10g/L gradually from 4g/L
After tolerance of salinity domestication, choose the microscopy activity the algae kind of strong (chromatoplast is more complete, and cellular form is outstanding) do further domestication.
(2). progressively reduce the content of macroelement such as nitrogen, phosphorus in the substratum, make the final concentration of elements such as nitrogen phosphorus be reduced to the concentration of local water quality, trace element does not add.
Sodium bicarbonate 5-35g/L sodium-chlor 8-10g/L
Yellow soda ash 1-9g/L sal epsom 0.05-0.2g/L
Calcium chloride 0.01-0.09g/L Plant hormones regulators,gibberellins 0.1-0.3mg/L
To tame dipotassium hydrogen phosphate in the substratum with reference to the phosphorus content in the pending water quality and be reduced to 5-2 times of phosphorus content in the pending water quality step by step;
The 5-2 that SODIUMNITRATE is reduced to nitrogen content the pending water quality from domestication substratum starting point concentration step by step with reference to the nitrogen content in the pending water quality doubly;
Culture condition through after the domestication of 3-5 growth cycle, is chosen the stronger algae kind of microscopy activity and is done further domestication under physical environment.
(3) will take from local water source through sterile filtration, as the domestication substratum, the nutritive element of substratum is the main nutritive element in local water source, and culture condition is under the physical environment, through after the domestication of 3-5 growth cycle, choose the stronger algae kind of microscopy activity and do further domestication.
(4) with local water source, be left intact, as the domestication substratum, the algal species cultivation condition is under the physical environment, and through the 3-5 growth cycle, the domestication stage finishes.
Step 3: the algae kind after will taming expands numerous cultivation in the laboratory: adopting local water source is 1 with algae kind and substratum by volume: 5-1: 7 ratio inoculation, the nutrient solution system is 60-100L, culture condition is: temperature 25-30 ℃, illumination 20000-35000 lux, pH is 8.5-10, cultivate after 6-9 days in closed reaction vessel, it is 1.5-3.0g/L that the spirulina in the nutrient solution reaches dry cell weight.
Step 4: with this algae kind in 1: the ratio of 100-200 is put in the both culturing microalgae pond, and the pond volume is preferably at 12-18m
3, open-air atmosphere was cultured 9-14 days down, and the spirulina cells dry weight is 1.2-2.7g/L in the pond, with little algae of pond culture provenance as alkaline land improving.
Step 5: will build the dam fence around the modified unit, saltings, with (50-100) * (50-100) rice is a processing unit, the preferred 30-40cm of the height of building a dam, utilize conventional irrigation and drainage facility, the water source of locality is introduced in the processing unit in saltings, keeping water surface degree of depth 4-6cm is advisable, be provided with little algae inlet tube in the processing unit of the present invention, so that the spirulina after the enlarged culturing is incorporated into the processing unit of saltings from the pond culture system, little algae inlet tube is advisable apart from floor level 30-50cm, by the flow velocity of little algae inlet tube the spirulina throwing is sprinkling upon in the saltings with 40-60L/min, generally speaking, each processing unit algae kind inoculum size is 0.03-0.06g/m
2, preferred 0.04-0.06g/m
2In the distance ground degree of depth is 0.2-0.8 rice, be provided with water table aquifer water shoot (hidden pipe), can be arranged in parallel between the water shoot, also can be crisscross arranged, or being provided with all around in processing unit, the water shoot spacing between pipes is a 5-8 rice when be arrangeding in parallel, valve is installed on the water shoot, on water shoot, have water port every 1-2 rice, so that can thoroughly drain the moisture of culturing spirulina, water shoot is communicated with the waterways of irrigation and drainage system, and the degree of depth in waterways needs the degree of depth greater than water shoot, drainage pipeline can be selected resistance to compression and anti-saline alkali corrosion material, as PVC material etc.;
Because the optimal growth water temperature of spirulina is 28～35 ℃, can carry out in spring, summer, autumn so handle the saltings, as run into the extreme climate influence with spirulina, can strengthen algae kind input amount or prolong the micro algae growth cycle.
Handle the saltings with the inventive method, the fertility of saltings topsoil is obviously increased, simultaneously, the cultivating microalgae water also can be taken away a large amount of salts in the saltings when discharging, after measured, after once little algae is handled, nitrogen content in the saltings, increased 21-23% than the soil of handling without little algae, phosphorus has increased 11-14%, the total salt quantity 20-25% that descended, and utilize chemical process to handle, obtain identical getting fat effect, need on equal area, apply phosphogypsum or the urea of 70-90kg, the present invention can carry out repeatedly little algae and handles according to waiting to plant the needs of plant to soil, to increase the micro algae biomass in the processing unit, improves the improve effect of system of units to the saltings.Handle the saltings with the inventive method, can effectively reduce treatment cost, the improving technology of little algae has not only increased the nitrogen and phosphorus content in the soil, the soil nutritive element of also having avoided the physics WATER-WASHING METHOD to cause is lost, the secondary pollution that can effectively avoid hardening function that the long-term application chemical fertilizer causes the soil again and water source and environment are caused.If life-time service the inventive method improvement saltings can progressively increase soil fertility, improve planting effect.The present invention handles the method in saltings, will recover for accelerating alkaline land soil, enlarge the acreage under cultivation of coastland and then improve whole saline and alkaline geographic ecotope and make positive contribution.
Description of drawings:
Fig. 1 spirulina improvement saltings process flow diagram
The comparison of growth of spirulina platensis situation before and after Fig. 2 tames
Nitrogen content changes before and after improveing among Fig. 3 heavy salinizedly
Phosphorus content changes before and after improveing among Fig. 3 heavy salinizedly
Embodiment
Handle peaceful town, the Binhai New Area in Tianjin plant saline and alkaline area of periphery
Step 1: the FN32 substratum (basic medium) of spirulina is cultured in preparation:
Sodium bicarbonate 10g/L sodium-chlor 1.5g/L
Yellow soda ash 2.5g/L sal epsom 0.1g/L
Dipotassium hydrogen phosphate 0.4g/L calcium chloride 0.05g/L
SODIUMNITRATE 1.5g/L Plant hormones regulators,gibberellins 0.1mg/L
Trace element 1.5ml/L
Wherein trace element formula is:
Boric acid 2.86mg iron(ic) chloride 350mg
Zinc sulfate 0.12mg Sodium orthomolybdate 20.4mg
Manganous sulfate 1.36mg cobalt chloride 6.5mg
Copper sulfate 0.047mg adding distil water is settled to 1L
Culture condition is: 30 ℃ of temperature, illumination 15000 luxs, pH:9.0, growth cycle 7 days.
Step 2: the purposes by the saltings is carried out the adaptability domestication to spirulina:
(1). tolerance of salinity domestication: at the situation on moderate or heavy salinized ground, spirulina is adapted to the cultivation of saline and alkaline, make it can adapt to more and more higher salinity gradually, and still can keep the high-speed rapid growth breeding, its tolerance of salinity domestication culture medium prescription is
Sodium bicarbonate 10g/L sal epsom 0.1g/L
Yellow soda ash 2.5g/L calcium chloride 0.05g/L
Dipotassium hydrogen phosphate 0.4g/L Plant hormones regulators,gibberellins 0.1mg/L
SODIUMNITRATE 1.5g/L trace element 1.5ml/L
Sodium-chlor is increased to 9g/L gradually from 4
After tolerance of salinity domestication, the salt tolerance of spirulina obviously increases, in containing the domestication substratum of 9g/L sodium-chlor, cultivated 8 days, the algae kind is than the spirulina without domestication, dry cell weight has increased about 80% (Fig. 2), choose the algae kind of microscopy activity strong (chromatoplast is more complete, and cellular form is outstanding) and do further domestication.
(2). progressively reduce the content of macroelement such as nitrogen, phosphorus in the substratum, make the final concentration of elements such as nitrogen phosphorus be reduced to the concentration of local water quality:
Sodium bicarbonate 10g/L sodium-chlor 9.0g/L
Yellow soda ash 2.5g/L sal epsom 0.1g/L
Calcium chloride 0.05g/L Plant hormones regulators,gibberellins 0.1mg/L
SODIUMNITRATE is reduced to 5.2mg/L with reference to the nitrogen content in the local water quality step by step from domestication substratum starting point concentration
Dipotassium hydrogen phosphate is reduced to 0.6mg/L with reference to the phosphorus content in the local water quality step by step from domestication substratum starting point concentration
Culture condition after the domestication through 4 growth cycles, is chosen the stronger algae kind of microscopy activity and is done further domestication under physical environment.
(3). will take from local water source through sterile filtration, as the domestication substratum, the nutritive element of substratum is the main nutritive element in local water source, and culture condition is under the physical environment, through after the domestication of 3-5 growth cycle, choose the stronger algae kind of microscopy activity and do further domestication.
(4) with local water source, be left intact, as the domestication substratum, the algal species cultivation condition is under the physical environment, and through the 3-5 growth cycle, the domestication stage finishes.
Step 3: the algae kind after will taming expands numerous cultivation in the laboratory: adopting local water source is 1: 5 ratio inoculation by volume with algae kind and substratum, the nutrient solution system is 70L, culture condition is: 30 ℃ of temperature, illumination 30000 luxs, pH is 9.0, cultivate after 6 days in closed reaction vessel, it is 2.2-3.0g/L that the spirulina in the nutrient solution reaches dry cell weight.
Step 4: this algae kind is put in the both culturing microalgae pond in 1: 150 ratio, and the pond volume is preferably at 12m
3, open-air atmosphere was cultured 12 days down, and the spirulina cells dry weight is 1.2-1.8g/L in the pond, with little algae of pond culture provenance as alkaline land improving.
Step 5: will build the dam fence around the modified unit, saltings, with 80 * 80 meters is a processing unit, the preferred 35cm of the height of building a dam, utilize conventional irrigation and drainage facility, the water source of locality is introduced in the processing unit in saltings, kept water surface degree of depth 4cm and be advisable, the little algae after the enlarged culturing is incorporated into the processing unit of saltings from the pond culture system by little algae inlet tube, little algae flow velocity is 50L/min, and each processing unit algae kind inoculum size is 0.05g/m
2In the distance ground degree of depth is 0.8 meter, is provided with the water table aquifer water shoot, and valve is installed on the water shoot, has water port every 2 meters on water shoot, be arranged in parallel between the water shoot, and spacing between pipes is 6 meters.
Because the optimal growth temperature of spirulina is 25～30 ℃, can carry out in spring, summer, autumn so handle the saltings, as run into the extreme climate influence with spirulina, can strengthen algae kind input amount or prolong the micro algae growth cycle.
Handle the saltings with the inventive method, the fertility of saltings topsoil is obviously increased, simultaneously, the cultivating microalgae water also can be taken away a large amount of salts in the saltings when discharging, after measured, and after once little algae is handled, nitrogen content in the saltings, increased by 22% than the soil of handling without little algae, phosphorus has increased by 12%, and total salt quantity has descended 22%.(seeing Fig. 3-4)
Handle peaceful town, the Binhai New Area in Tianjin plant saline and alkaline area of periphery
Step 1: the substratum (basic medium) of spirulina is cultured in preparation:
Sodium bicarbonate 12g/L sodium-chlor 2.0g/L
Yellow soda ash 3.5g/L sal epsom 0.15g/L
Dipotassium hydrogen phosphate 0.6g/L calcium chloride 0.02g/L
SODIUMNITRATE 3.5g/L Plant hormones regulators,gibberellins 0.2mg/L
Trace element 0.5ml/L
Culture condition is: 28 ℃ of temperature, illumination 20000 luxs, pH:9.5, growth cycle 6 days.
Step 2: the purposes by the saltings is carried out the adaptability domestication to spirulina:
(1). tolerance of salinity domestication:
Sodium bicarbonate 12g/L sal epsom 0.15g/L
Yellow soda ash 3.5g/L calcium chloride 0.02g/L
Dipotassium hydrogen phosphate 0.6g/L Plant hormones regulators,gibberellins 0.2mg/L
SODIUMNITRATE 3.5g/L trace element 0.5ml/L
Sodium-chlor is increased to 10g/L gradually from 4g/L
After tolerance of salinity domestication, choose the microscopy activity the algae kind of strong (chromatoplast is more complete, and cellular form is outstanding) do further domestication.
(2). the content culture medium prescription that progressively reduces macroelement such as nitrogen, phosphorus in the substratum is adjusted into:
Sodium bicarbonate 12g/L sodium-chlor 10g/L
Yellow soda ash 3.5g/L sal epsom 0.15g/L
Calcium chloride 0.02g/L Plant hormones regulators,gibberellins 0.2mg/L
SODIUMNITRATE is reduced to 7.8mg/L with reference to the nitrogen content in the local water quality step by step from domestication substratum starting point concentration
Dipotassium hydrogen phosphate is reduced to 0.9mg/L with reference to the phosphorus content in the local water quality step by step from domestication substratum starting point concentration
Culture condition after the domestication through 5 growth cycles, is chosen the stronger algae kind of microscopy activity and is done further domestication under physical environment.
(3). will take from local water source through sterile filtration, and further tame as the domestication substratum, method is with embodiment 1;
(4) with local water source, further to tame as the domestication substratum, method is with embodiment 1;
Step 3: the algae kind after will taming expands numerous cultivation in the laboratory: adopting local water source is 1: 7 ratio inoculation by volume with algae kind and substratum, the nutrient solution system is 90L, cultivate after 9 days in closed reaction vessel, it is 1.5-2.4g/L that the spirulina in the nutrient solution reaches dry cell weight.
Step 4: this algae kind is put in the both culturing microalgae pond in 1: 100 ratio, and the pond volume is preferably at 15m
3, open-air atmosphere was cultured 9 days down, and the spirulina cells dry weight is 1.4-2.2g/L in the pond, with little algae of pond culture provenance as alkaline land improving.
Step 5: with 80 * 80 meters be a processing unit, keep water surface degree of depth 6cm, spirulina is introduced processing unit with the flow velocity of 40L/min, each processing unit algae kind inoculum size is 0.04g/m
2, around water shoot is arranged in the processing unit, have water port every 1.5 meters on the water shoot, be 0.3 meter apart from the ground degree of depth.
After spirulina was handled, the nitrogen content in the saltings had increased by 21% than the soil of handling without little algae, and phosphorus has increased by 11%, and total salt quantity has descended 25%.
Embodiment 3:
Handle peaceful town, the Binhai New Area in Tianjin plant saline and alkaline area of periphery
Step 1: the substratum (basic medium) of spirulina is cultured in preparation:
Sodium bicarbonate 15g/L sodium-chlor 1.0g/L
Yellow soda ash 5g/L sal epsom 0.05g/L
Dipotassium hydrogen phosphate 0.3g/L calcium chloride 0.03g/L
SODIUMNITRATE 1.0g/L Plant hormones regulators,gibberellins 0.3mg/L
Trace element 1ml/L
Culture condition is: 29 ℃ of temperature, illumination 25000 luxs, pH:9.0, growth cycle 8 days.
Step 2: the purposes by the saltings is carried out the adaptability domestication to spirulina:
(1). tolerance of salinity domestication:
Sodium bicarbonate 15g/L sal epsom 0.05g/L
Yellow soda ash 5g/L calcium chloride 0.03g/L
Dipotassium hydrogen phosphate 0.3g/L Plant hormones regulators,gibberellins 0.3mg/L
SODIUMNITRATE 1g/L trace element 1ml
Sodium-chlor is increased to 8g/L gradually from 4g/L
After tolerance of salinity domestication, choose the microscopy activity the algae kind of strong (chromatoplast is more complete, and cellular form is outstanding) do further domestication.
(2). progressively reduce the content of macroelement such as nitrogen, phosphorus in the substratum, culture medium prescription is adjusted into:
Sodium bicarbonate 15g/L sodium-chlor 8.0g/L
Yellow soda ash 5g/L sal epsom 0.05g/L
Calcium chloride 0.03g/L Plant hormones regulators,gibberellins 0.3mg/L
SODIUMNITRATE is reduced to 13mg/L with reference to the nitrogen content in the local water quality step by step from domestication substratum starting point concentration
Dipotassium hydrogen phosphate is reduced to 1.5mg/L with reference to the phosphorus content in the local water quality step by step from domestication substratum starting point concentration
Culture condition after the domestication through 3 growth cycles, is chosen the stronger algae kind of microscopy activity and is done further domestication under physical environment.
(3). will take from local water source through sterile filtration, and further tame as the domestication substratum, method is with embodiment 1;
(4) with local water source, further to tame as the domestication substratum, method is with embodiment 1;
Step 3: the algae kind after will taming expands numerous cultivation in the laboratory: adopting local water source is 1: 6 ratio inoculation by volume with algae kind and substratum, the nutrient solution system is 80L, cultivate after 7 days in closed reaction vessel, it is 1.8-2.6g/L that the spirulina in the nutrient solution reaches dry cell weight.
Step 4: this algae kind is put in the both culturing microalgae pond in 1: 200 ratio, and the pond volume is preferably at 18m
3, open-air atmosphere was cultured 14 days down, and the dry cell weight of spirulina is 1.6-2.7g/L in the pond, with little algae of pond culture provenance as alkaline land improving.
Step 5: with 80 * 80 meters be a processing unit, keep water surface degree of depth 5cm, spirulina is introduced processing unit with the flow velocity of 60L/min, each processing unit algae kind inoculum size is 0.06g/m
2, having water port every 1.5 meters on the water shoot, water shoot is 0.5 meter apart from the ground degree of depth, spacing between pipes is 5 meters,
After step 6, little algae grew through 10 days, the valve on the opening water discharge pipe drained residue moisture content, and the little algae improved, process of this round finishes.
After spirulina was handled, the nitrogen content in the saltings had increased by 23% than the soil of handling without little algae, and phosphorus has increased by 14%, and total salt quantity has descended 23%.
Claims (10)
1. the cultural method of a spirulina is characterized in that comprising the steps:
(1) screening algae kind;
(2) the FN32 substratum of spirulina is cultured in preparation, and its prescription is:
Sodium bicarbonate 5-35g/L sodium-chlor 0.5-3.0g/L
Yellow soda ash 1-9g/L sal epsom 0.05-0.2g/L
Dipotassium hydrogen phosphate 0.1-1.8g/L calcium chloride 0.01-0.09g/L
SODIUMNITRATE 0.8-4.0g/L Plant hormones regulators,gibberellins 0.05-0.4mg/L
Trace element 0.5-2ml/L
Wherein micro-compound method is:
Boric acid 1-5mg iron(ic) chloride 150-800mg
Zinc sulfate 0.04-0.3mg Sodium orthomolybdate 5-40mg
Manganous sulfate 0.35-2.5mg cobalt chloride 3.5-10mg
Copper sulfate 0.02-0.09mg adding distil water is settled to 1L;
(3) culture condition is: temperature 25-30 ℃, and illumination 10000-30000 lux, pH:8.5～10, growth cycle 5-8 days.
2. the cultural method of spirulina as claimed in claim 1, its preferably culture medium prescription be:
Sodium bicarbonate 10-15g/L sodium-chlor 1.0-2.0g/L
Yellow soda ash 2.5-5g/L sal epsom 0.05-0.15g/L
Dipotassium hydrogen phosphate 0.3-0.6g/L calcium chloride 0.02-0.05g/L
SODIUMNITRATE 1-3.5g/L Plant hormones regulators,gibberellins 0.1-0.3mg/L
Trace element 0.5-1.5ml/L
3. as the acclimation method of claim 1,2 described spirulinas, it is characterized in that comprising the steps:
(1) sodium-chlor in the substratum is increased to 10g/L gradually from 4g/L, carries out tolerance of salinity domestication, afterwards, choose the stronger algae kind of microscopy activity and do further domestication;
(2) progressively reduce the content of nitrogen in the substratum, phosphoric, make 5-2 that the final concentration of nitrogen, phosphoric is reduced to nitrogen, phosphorus concentration in the pending regional water quality doubly, under physical environment,, choose the stronger algae kind of microscopy activity and do further domestication through after the domestication of 3-5 growth cycle;
(3) will take from pending geographic water source through sterile filtration, and, under physical environment,, choose the stronger algae kind of microscopy activity and do further domestication through after the domestication of 3-5 growth cycle as substratum;
(4) with local water source,, under physical environment, carry out the domestication of 3-5 growth cycle as substratum;
4. spirulina as claimed in claim 3 is handled the method in saltings, it is characterized in that comprising the steps:
(1) spirulina algae kind after will taming and pending regional water source are by 1: 5-1: 7 volume ratio inoculation, in closed reaction vessel, cultivated 6-9 days, and expand numerous;
(2) will expand algae kind after numerous cultivation in 1: the ratio of 100-200 is put in the both culturing microalgae pond, and open-air atmosphere was cultured 9-14 days down;
(3) build the dam fence around in unit, saltings to be improved, the water source of locality is introduced in the processing unit in saltings;
(4) little algae inlet tube is set in processing unit, the spirulina throwing is sprinkling upon in the saltings by the flow velocity of little algae inlet tube with 40-60L/min;
(5) be 0.2-0.8 rice in the distance ground degree of depth, water shoot is set, valve is installed on the water shoot, water shoot is communicated with the waterways of irrigation and drainage system;
(6) through after the growth breeding in 9-12 days, when the biomass for the treatment of spirulina was 1.0-1.8g (dry weight)/L, the valve on the opening water discharge pipe drained residue moisture content to spirulina, makes spirulina be deposited on upper soll layer in the saltings, and the little algae improved, process of this round finishes.
5. spirulina as claimed in claim 4 is handled the method in saltings, and it is characterized in that expanding numerous culture temperature is 25-30 ℃, and illumination is the 20000-35000 lux, and pH is 8.5～10.
6. spirulina as claimed in claim 4 is handled the method in saltings, it is characterized in that the pond volume is preferably at 12-18m
3
7. spirulina as claimed in claim 4 is handled the method in saltings, it is characterized in that the preferred 30-40cm of height that the saltings processing unit is built a dam, and introduces the preferred 5-8cm of the depth of water in saltings,
8. spirulina as claimed in claim 4 is handled the method in saltings, it is characterized in that each processing unit algae kind inoculum size is 0.03-0.06g/m
2, preferred 0.04-0.06g/m
2
9. spirulina as claimed in claim 4 is handled the method in saltings, it is characterized in that can be arrangeding in parallel between the water shoot, also can be crisscross arranged, or being provided with all around in processing unit, have water port every 1-2 rice on the water shoot, spacing between pipes was a 5-8 rice when water shoot be arranged in parallel.
10. spirulina as claimed in claim 4 is handled the method in saltings, it is characterized in that the material of drainage pipeline selection resistance to compression and anti-saline alkali corrosion, preferred PVC material.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102344893A (en) * | 2011-08-04 | 2012-02-08 | 双赢集团有限公司 | Screening method of diammonium phosphate tolerant bacillus subtilis |
| CN104593314A (en) * | 2014-12-31 | 2015-05-06 | 浙江大学 | Method for improving micro algae growth rate and cell fat content employing high-salinity domestication |
| CN105483062A (en) * | 2016-02-02 | 2016-04-13 | 杭州优普西生物科技有限公司 | Microelement-rich spirulina culture medium |
| CN108441228A (en) * | 2018-04-03 | 2018-08-24 | 广东丰康生物科技有限公司 | A kind of environment protection type soil modifying agent |
| CN108795798A (en) * | 2018-05-28 | 2018-11-13 | 陈顺金 | A kind of trace element bioconversion method |
| CN108991281A (en) * | 2018-07-09 | 2018-12-14 | 江继永 | A method of koi feed is produced using Pig farm wastewater cultivating microalgae |
| CN109354199A (en) * | 2018-11-27 | 2019-02-19 | 海南大学 | Spirulina effluent treatment plant for the coastal eutrophication salt water lake in the torrid zone |
| WO2019085280A1 (en) * | 2017-11-03 | 2019-05-09 | 中国科学院生态环境研究中心 | Multi-pond-algae water circulating irrigation system and method for remedying polluted soil using same |
| CN114467397A (en) * | 2022-02-11 | 2022-05-13 | 山东大学 | System and method for improving saline-alkali soil in situ by using microalgae |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07213279A (en) * | 1994-01-31 | 1995-08-15 | Kanagawa Pref Gov | Microorganism capable of improving strong acid soil and method therefor |
| WO2000029546A1 (en) * | 1998-11-16 | 2000-05-25 | Succeed Hi-Tech Industrialization Scale-Up Assemblies Co., Ltd. | Method of cultivating fresh spirulina at home and device thereof |
| JP2005230000A (en) * | 2004-02-19 | 2005-09-02 | Abe Sogyo:Kk | Soil improving method utilizing wood chip and lactic acid bacteria |
| CN201530825U (en) * | 2009-07-06 | 2010-07-21 | 苏勇宁 | Spiral algae culturing device |
-
2010
- 2010-08-26 CN CN 201010263524 patent/CN102010830B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07213279A (en) * | 1994-01-31 | 1995-08-15 | Kanagawa Pref Gov | Microorganism capable of improving strong acid soil and method therefor |
| WO2000029546A1 (en) * | 1998-11-16 | 2000-05-25 | Succeed Hi-Tech Industrialization Scale-Up Assemblies Co., Ltd. | Method of cultivating fresh spirulina at home and device thereof |
| JP2005230000A (en) * | 2004-02-19 | 2005-09-02 | Abe Sogyo:Kk | Soil improving method utilizing wood chip and lactic acid bacteria |
| CN201530825U (en) * | 2009-07-06 | 2010-07-21 | 苏勇宁 | Spiral algae culturing device |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102344893A (en) * | 2011-08-04 | 2012-02-08 | 双赢集团有限公司 | Screening method of diammonium phosphate tolerant bacillus subtilis |
| CN104593314A (en) * | 2014-12-31 | 2015-05-06 | 浙江大学 | Method for improving micro algae growth rate and cell fat content employing high-salinity domestication |
| CN105483062A (en) * | 2016-02-02 | 2016-04-13 | 杭州优普西生物科技有限公司 | Microelement-rich spirulina culture medium |
| WO2019085280A1 (en) * | 2017-11-03 | 2019-05-09 | 中国科学院生态环境研究中心 | Multi-pond-algae water circulating irrigation system and method for remedying polluted soil using same |
| CN108441228A (en) * | 2018-04-03 | 2018-08-24 | 广东丰康生物科技有限公司 | A kind of environment protection type soil modifying agent |
| CN108795798A (en) * | 2018-05-28 | 2018-11-13 | 陈顺金 | A kind of trace element bioconversion method |
| CN108991281A (en) * | 2018-07-09 | 2018-12-14 | 江继永 | A method of koi feed is produced using Pig farm wastewater cultivating microalgae |
| CN109354199A (en) * | 2018-11-27 | 2019-02-19 | 海南大学 | Spirulina effluent treatment plant for the coastal eutrophication salt water lake in the torrid zone |
| CN109354199B (en) * | 2018-11-27 | 2021-08-31 | 海南大学 | Spirulina sewage purification device for tropical coastal eutrophic salt water lake |
| CN114467397A (en) * | 2022-02-11 | 2022-05-13 | 山东大学 | System and method for improving saline-alkali soil in situ by using microalgae |
| CN114467397B (en) * | 2022-02-11 | 2022-08-02 | 山东大学 | System and method for improving saline-alkali soil in situ by using microalgae |
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