CN101986859A - Method for preparing II type collagen mixtures by using poultry chest cartilage as raw materials - Google Patents
Method for preparing II type collagen mixtures by using poultry chest cartilage as raw materials Download PDFInfo
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- CN101986859A CN101986859A CN 201010511582 CN201010511582A CN101986859A CN 101986859 A CN101986859 A CN 101986859A CN 201010511582 CN201010511582 CN 201010511582 CN 201010511582 A CN201010511582 A CN 201010511582A CN 101986859 A CN101986859 A CN 101986859A
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Abstract
The invention relates to a method for preparing II type collagen mixtures by using poultry chest cartilage as raw materials, which comprises the following steps of: 1. putting the poultry chest cartilage and purified water according to the weight ratio of 1:1 to 1.2, and boiling the materials for 4 to 8 hours under normal pressure until protein combination bodies are primarily degraded; 2. reducing the temperature to the zymohydrolysis temperature between 50 and 55 DEG C, adding protamex accounting for 0.08 to 0.12 percent of the total weight of the cartilage, then adding flavourzyme accounting for 0.12 to 0.18 percent of the total weight of the cartilage after the zymohydrolysis is subjected to 0.5 to 1.5 hours, and carrying out zymohydrolysis for 2 to 3 hours; 3. ultrafiltering clear liquid by filter membranes for primarily concentrating the clear liquid and removing salts; 4. concentrating the primarily concentrated liquid in the vacuum degree between 0.07 and 0.1 MPa so that the solid content reaches more than 50 percent; and 5. carrying out spray drying on the concentrated liquid obtained in Step 4 to obtain the II type collagen mixtures. The method has the advantages of rich raw material resource, simple and convenient processing, large-scale production, short period, low cost, good quality and the like.
Description
Technical field
The invention belongs to food technology field, being specifically related to a kind of is the method for feedstock production II collagen type mixture with bird chest cartilage.
Technical background
The II collagen type is as natural living resources, particular structure and function, make it have good Bc and biodegradability, with and natural hypotoxicity, hypoimmunity, be widely used in fields such as biomedicine, food industry, cosmetics.Mainly have following function: (1) participates in human body immune system function, safeguard articulation health, increase the flexibility and the adaptability in joint, the aspects such as pressure in bone and joint are played an important role keeping human body proper motion function, joint mobility, buffering body weight and external force; (2) reducing triglycerides and cholesterol in the blood, and can increase the essential trace element of some shortage in the body, keep stable state, is a kind of desirable reducing weight and blood fat food; (3) quicken hemoglobin and erythropoietic effect, improve circulation, favourable coronary heart disease, ischemic cerebral disease; (4) be rich in glycine, in human body, not only participate in synthetic collagen, but also be to be a kind of nervous centralis inhibitory transmitter in the brain cell,, anxiety disorder, neurasthenia etc. is had the good curing effect to produce sedation to nervous centralis; (5) be a kind of good merchantable brand of replenishing the calcium.The hydroxy-proline that comes from collagen in the blood plasma is the delivery vehicle that the calcium in the blood plasma is transported to osteocyte, and the collagen in the osteocyte then is the adhesive of hydroxyapatite, and it and hydroxyapatite have constituted the main body of bone jointly.Therefore, as long as take in enough collagens, just can guarantee normal calcareous intake; (6) its catabolite collagen polypeptide can directly be absorbed at enteron aisle, the peptide class is absorbed with different approach with amino acid, and peptide analogy amino acid has, and speed is fast, power consumption is low, it is saturated to be difficult for, and characteristics such as uncontested property of running and inhibition between the various peptide.Therefore, the continuous excavation along with II collagen type functional characteristics promotes the well-being of mankind it widely.
Contain II collagen type 48～62% in the bird chest cartilage cartilage, proteoglycans 22～38%, hyaluronic acid 1%.The main matter basis that these biotic component major parts are human life activities, however majority is present in the macromolecular collagen protein combination framework, is difficult for being absorbed by human consumption and utilizing.In addition, existing II collagen type preparation method's production cycle is long, complex operation, and cost is higher, and unstable product quality is unfavorable for accomplishing scale production.
Summary of the invention
The objective of the invention is to overcome the above-mentioned problems in the prior art, providing a kind of is the method for feedstock production II collagen type mixture with bird chest cartilage.Its raw material sources are abundant, process easy again can large-scale production, the cycle lacks, cost is low, quality better.
For achieving the above object, the technical solution used in the present invention is as follows:
Of the present invention is the method for feedstock production II collagen type mixture with bird chest cartilage, and this method is undertaken by following step:
(1) with bird chest cartilage and purified water by weight for the ratio of 1:1 ~ 1.2 feeds intake, normal pressure boils tentatively degraded to the protein combination body in 4 ~ 8 hours;
(2) be cooled to 50～55 ℃ hydrolysis temperature, add the Protamex enzyme that accounts for cartilage gross weight 0.08～0.12%, enzymolysis adds after 0.5 ~ 1.5 hour again and accounts for cartilage gross weight 0.12～0.18% Flavourzyme enzyme, enzymolysis 2 ~ 3 hours;
(4) with filter membrane clear liquid is carried out ultrafiltration, make clear liquid tentatively concentrated, and remove freshen;
(5) preliminary concentrate is concentrated under 0.07 ~ 0.1Mpa vacuum, solid contents is reached more than 50%;
(6) concentrate that step (5) is obtained carries out spray-drying, obtains II collagen type mixture.
In the step (2), the addition of described Protamex enzyme accounts for 0.1% of chondroitin gross weight.
In the step (2), the addition of described Flavourzyme enzyme accounts for 0.16% of chondroitin gross weight.
At described raw material bird chest cartilage, be from Pest-or disease-free area, fresh no corruption; Used water should be purified water.
The as easy as rolling off a log water of the final powder of this product, and as clear as crystal; Be rich in multiple nutritional components, II collagen type 60～70%, chondroitin sulfate 20～30%, hyaluronic acid 8～15%, ash content are below 8%, and total number of bacteria is below 1000 cfu/g.
The II collagen type of preparing by the present invention, the II collagen type that has kept all biological activity, have closely similar institutional framework and antigenic structure with human articular cartilage, not only be rich in asparatate, glycine, glutamic acid and arginine, also contain mucopolysaccharides such as chondroitin sulfate, hyaluronic acid, also contain the mineral elements such as calcium, magnesium and zinc of needed by human.Processing method of the present invention, raw material sources are abundant, process easy again can large-scale production, the cycle lacks, cost is low, quality better.
Description of drawings
Fig. 1 is a process chart of the present invention.
The specific embodiment
Embodiment 1
(1) with bird chest cartilage and purified water by weight for the ratio of 1:1 ~ 1.2 feeds intake, normal pressure boils tentatively degraded to the protein combination body in 4 ~ 8 hours;
(2) be cooled to 50～55 ℃ hydrolysis temperature, add the Protamex enzyme that accounts for cartilage gross weight 0.08～0.12%, enzymolysis adds after 0.5 ~ 1.5 hour again and accounts for cartilage gross weight 0.12～0.18% Flavourzyme enzyme, enzymolysis 2 ~ 3 hours;
(4) with filter membrane clear liquid is carried out ultrafiltration, make clear liquid tentatively concentrated, and remove freshen;
(5) preliminary concentrate is concentrated under 0.07 ~ 0.1Mpa vacuum, solid contents is reached more than 50%;
(6) concentrate that step (5) is obtained carries out spray-drying, obtains II collagen type mixture.
(7) II collagen type powder is packed with the double-layer plastic bag, and sealing is tight, forbids the moisture absorption.
At described raw material bird chest cartilage, be from Pest-or disease-free area, fresh no corruption; Used water should be purified water.
Final powder speed is water-soluble, and as clear as crystal; II collagen type 60～70%, chondroitin sulfate 20～30%, hyaluronic acid 8～15%, ash content are below 8%, and total number of bacteria is below 1000 cfu/g.
Embodiment 2
(1) with bird chest cartilage and purified water by weight for the ratio of 1:1 feeds intake, normal pressure boils tentatively degraded to the protein combination body in 4 hours;
(2) be cooled to 50 ℃ hydrolysis temperature, add the Protamex enzyme that accounts for cartilage gross weight 0.08%, enzymolysis adds after 0.5 hour again and accounts for cartilage gross weight 0.12% Flavourzyme enzyme, enzymolysis 3 hours;
(4) with filter membrane clear liquid is carried out ultrafiltration, make clear liquid tentatively concentrated, and remove freshen;
(5) preliminary concentrate is concentrated under 0.07Mpa vacuum, solid contents is reached more than 50%;
(6) concentrate that step (5) is obtained carries out spray-drying, obtains II collagen type mixture.
(7) II collagen type powder is packed with the double-layer plastic bag, and sealing is tight, forbids the moisture absorption.
At described raw material bird chest cartilage, be from Pest-or disease-free area, fresh no corruption.
Final powder speed is water-soluble, and as clear as crystal; II collagen type 62%, chondroitin sulfate 26%, hyaluronic acid 9.2%, ash content 6.3%, total number of bacteria 300 cfu/g.
Embodiment 3
(1) with bird chest cartilage and purified water by weight for the ratio of 1:1.1 feeds intake, normal pressure boils tentatively degraded to the protein combination body in 6 hours;
(2) be cooled to 52 ℃ hydrolysis temperature, add the Protamex enzyme that accounts for cartilage gross weight 0.10%, enzymolysis adds after 1 hour again and accounts for cartilage gross weight 0.16% Flavourzyme enzyme, enzymolysis 2.5 hours;
(4) with filter membrane clear liquid is carried out ultrafiltration, make clear liquid tentatively concentrated, and remove freshen;
(5) preliminary concentrate is concentrated under 0.08Mpa vacuum, solid contents is reached more than 50%;
(6) concentrate that step (5) is obtained carries out spray-drying, obtains II collagen type mixture.
(7) II collagen type powder is packed with the double-layer plastic bag, and sealing is tight, forbids the moisture absorption.
At described raw material bird chest cartilage, be from Pest-or disease-free area, fresh no corruption.
Final powder speed is water-soluble, and as clear as crystal; II collagen type 65%, chondroitin sulfate 30%, hyaluronic acid 12%, ash content 5.8%, total number of bacteria 500 cfu/g.
Embodiment 4
(1) with bird chest cartilage and purified water by weight for the ratio of 1:1.2 feeds intake, normal pressure boils tentatively degraded to the protein combination body in 8 hours;
(2) be cooled to 55 ℃ hydrolysis temperature, add the Protamex enzyme that accounts for cartilage gross weight 0.12%, enzymolysis adds after 1.5 hours again and accounts for cartilage gross weight 0.18% Flavourzyme enzyme, enzymolysis 2 hours;
(4) with filter membrane clear liquid is carried out ultrafiltration, make clear liquid tentatively concentrated, and remove freshen;
(5) preliminary concentrate is concentrated under 0.1Mpa vacuum, solid contents is reached more than 50%;
(6) concentrate that step (5) is obtained carries out spray-drying, obtains II collagen type mixture.
(7) II collagen type powder is packed with the double-layer plastic bag, and sealing is tight, forbids the moisture absorption.
At described raw material bird chest cartilage, be from Pest-or disease-free area, fresh no corruption.
Final powder speed is water-soluble, and as clear as crystal; II collagen type 70%, chondroitin sulfate 24%, hyaluronic acid 15%, ash content 6.6%, total number of bacteria 600 cfu/g.
Claims (3)
1. one kind is the method for feedstock production II collagen type mixture with bird chest cartilage, and it is characterized in that: this method is undertaken by following step:
(1) with bird chest cartilage and purified water by weight for the ratio of 1:1 ~ 1.2 feeds intake, normal pressure boils tentatively degraded to the protein combination body in 4 ~ 8 hours;
(2) be cooled to 50～55 ℃ hydrolysis temperature, add the Protamex enzyme that accounts for cartilage gross weight 0.08～0.12%, enzymolysis adds after 0.5 ~ 1.5 hour again and accounts for cartilage gross weight 0.12～0.18% Flavourzyme enzyme, enzymolysis 2 ~ 3 hours;
(4) with filter membrane clear liquid is carried out ultrafiltration, make clear liquid tentatively concentrated, and remove freshen;
(5) preliminary concentrate is concentrated under 0.07 ~ 0.1Mpa vacuum, solid contents is reached more than 50%;
(6) concentrate that step (5) is obtained carries out spray-drying, obtains II collagen type mixture.
2. according to claim 1 is the method for feedstock production II collagen type mixture with bird chest cartilage, and it is characterized in that: in the step (2), the addition of described Protamex enzyme accounts for 0.1% of chondroitin gross weight.
3. according to claim 1 is the method for feedstock production II collagen type mixture with bird chest cartilage, and it is characterized in that: in the step (2), the addition of described Flavourzyme enzyme accounts for 0.16% of chondroitin gross weight.
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| CN 201010511582 CN101986859A (en) | 2010-10-19 | 2010-10-19 | Method for preparing II type collagen mixtures by using poultry chest cartilage as raw materials |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399439A (en) * | 2016-11-04 | 2017-02-15 | 武汉九生堂生物科技股份有限公司 | Animal joint cartilage processed product as well as preparation method and application thereof |
| WO2022119508A1 (en) * | 2020-12-01 | 2022-06-09 | Gedi̇k Tavukçuluk Ve Tarim Ürünleri̇ Ti̇caret Sanayi̇ Anoni̇m Şi̇rketi̇ | Use of poultry cartilage as a food product |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1721546A (en) * | 2004-07-12 | 2006-01-18 | 周立军 | Process for preparing II-type collagen compound |
| CN101781672A (en) * | 2010-04-13 | 2010-07-21 | 北京华达杰瑞生物技术有限公司 | Process for preparing hydrolyzed II-type collagen |
| CN102108374A (en) * | 2009-12-24 | 2011-06-29 | 河南双汇投资发展股份有限公司 | Method for processing type-II collagen mixture by using poultry sternal cartilages as raw materials |
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- 2010-10-19 CN CN 201010511582 patent/CN101986859A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1721546A (en) * | 2004-07-12 | 2006-01-18 | 周立军 | Process for preparing II-type collagen compound |
| CN102108374A (en) * | 2009-12-24 | 2011-06-29 | 河南双汇投资发展股份有限公司 | Method for processing type-II collagen mixture by using poultry sternal cartilages as raw materials |
| CN101781672A (en) * | 2010-04-13 | 2010-07-21 | 北京华达杰瑞生物技术有限公司 | Process for preparing hydrolyzed II-type collagen |
Non-Patent Citations (1)
| Title |
|---|
| 《烟台大学学报（自然科学与工程版）》 20100430 郑明强 等 从鸡骨中提取骨胶原的酶解工艺研究 1-3 第23卷, 第2期 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399439A (en) * | 2016-11-04 | 2017-02-15 | 武汉九生堂生物科技股份有限公司 | Animal joint cartilage processed product as well as preparation method and application thereof |
| WO2022119508A1 (en) * | 2020-12-01 | 2022-06-09 | Gedi̇k Tavukçuluk Ve Tarim Ürünleri̇ Ti̇caret Sanayi̇ Anoni̇m Şi̇rketi̇ | Use of poultry cartilage as a food product |
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Application publication date: 20110323 |