CN101983701B - Traditional Chinese medicine compound preparation with protective effects on stomach and intestines - Google Patents
Traditional Chinese medicine compound preparation with protective effects on stomach and intestines Download PDFInfo
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- CN101983701B CN101983701B CN2010101748168A CN201010174816A CN101983701B CN 101983701 B CN101983701 B CN 101983701B CN 2010101748168 A CN2010101748168 A CN 2010101748168A CN 201010174816 A CN201010174816 A CN 201010174816A CN 101983701 B CN101983701 B CN 101983701B
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Abstract
The invention belongs to the technical field of research and development of medicine products, and relates to a traditional Chinese medicine compound preparation with protective effects on the stomach and intestines, which is prepared by cleaning, drying and pulverizing astragali, ginseng, orange peel, bamboo shavings, malt, hawthorn, jujube and the like, then extracting active components, bottling and sealing for use. The product of the invention has protective effects on the stomach mucous membrane and acceleration effects on the gastrointestinal motility, and the product of the invention is favorable for industrial production.
Description
Technical field
Medical product research and development technical field under the present invention.The present invention relates to a kind of compound Chinese medicinal preparation that gastrointestinal is had protective effect.It is that gastric mucosa is had protective effect and the gastrointestinal motility function is had facilitation that product of the present invention mainly acts on, and product of the present invention is fit to suitability for industrialized production.
Background technology
At present the reason that gastric ulcer is formed has multiple explanation, by the cell injury of Mediated by Free Radicals in the gastric mucosa damage always by extensive concern.Ethanol has fat-soluble, heavy dose of high concentration ethanol can directly destroy parietal cell, cause the blood flow retardance, make the histiocyte hypoxic-ischemic cause the gastric mucosa damage, and gastric mucosa is organized under the pathological conditions such as ischemia and can produces a large amount of oxygen-derived free radicals, the latter causes the peroxidating of mucosa cytolipin, causes tissue injury.In the stomach protection, SOD catalysis superoxide dismutase is a hydrogen peroxide, and the latter is a water by glutathion peroxidase catalysis, and the height of SOD vigor has reflected the ability of body removing oxygen-derived free radicals indirectly.And MDA is the harmful substance that lipid peroxidation produces, and content rises when alcohol induced gastric mucosa damage, and the height of MDA content has reflected the order of severity that body cell is attacked by oxygen-derived free radicals indirectly.
In this experiment, use 3850mgkg
-1And 7700mgkg
-1Compound Chinese medicinal preparation is handled all can significantly reduce the content that gastric mucosa is organized MDA, and at the compound Chinese medicinal preparation pretreatment 5d of the middle and high dosage of active function of SOD, be significantly increased, trend is opposite with MDA, show that lipid peroxidation has participated in the pathological process that ethanol causes the mice gastric injury, can damage the machine-processed consistent of gastric mucosa by free chain reaction with report ethanol such as Lee SO.The prompting compound Chinese medicinal preparation may reduce oxygen-derived free radicals and lipid peroxide and improve pathological conditions such as the histiocytic ischemia of the caused gastric mucosa of environmental stimuli, necrosis, the healing that helps damaging by improving the cell oxidation resistance.
Summary of the invention
1. the present invention relates to a kind ofly has the compound Chinese medicinal preparation of protective effect to gastrointestinal, it is characterized in that said preparation comprises following process:
Step (one): select high-quality Radix Astragali 6g-15g, Radix Ginseng 3g-9g, Pericarpium Citri tangerinae 3g-15g, Caulis Bambusae In Taenia 3g-9g, Fructus Hordei Germinatus 3g-15g, Fructus Crataegi 3g-9g, Fructus Jujubae 3g-6g and clean, microwave drying, pulverizing is 50 orders;
Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0-6.0h, heated and boiled is extracted 2.0-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0-1.5h, merge extractive liquid, filters, and gets filtrate;
Step (three): the filtrate in the step (two) is concentrated, get concentrated solution, concentration is 0.20g/mL crude drug amount, bottling, sealing;
Step (four): the concentrated solution in the step (three) is carried out gastrointestinal protective effect test, and this Chinese medicine compound concentrated solution preparation has protective effect and the gastrointestinal motility function is had facilitation gastric mucosa.
2. a kind of gastrointestinal to be had the primary raw material of the compound Chinese medicinal preparation of protective effect be the dry root of Radix Astragali Astragalus membranaceus (Fisch.) Bungede according to of the present invention; the dry root of Radix Ginseng Panax ginseng C.A.Mey.; the dry mature skin of Fructus Citri tangerinae Citrus reticulate Banco; Caulis Bambusae In Taenia is the intermediate layer of grass bambusa breviflora Munro Bambusa tuldoidesMunro stem, and Fructus Hordei Germinatus is that the mature fruit of grass Fructus Hordei Vulgaris Hordeum vulyare L. gets through the drying of germinateing; the mature fruit of the dry mature fruit of Fructus Crataegi Crataegus pinnatifida Bge. and Fructus Jujubae Ziziphus jujuba Mill.var.inermis (Bunge) Rehd..
According to the present invention, " % " among the present invention is percentage by weight.
The specific embodiment
Of the present invention relating to, a kind ofly have the compound Chinese medicinal preparation of protective effect to comprise following examples to gastrointestinal, and the following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
Step (one): select high-quality Radix Astragali 6g, Radix Ginseng 3g, Pericarpium Citri tangerinae 3g, Caulis Bambusae In Taenia 3g, Fructus Hordei Germinatus 3g, Fructus Crataegi 3g, Fructus Jujubae 3g and clean, microwave drying, pulverizing is 50 orders;
Step (two): the powder in the step () is added water boil extract 3 times, add 6 times of water gagings first and soak 4.0h, heated and boiled is extracted 2.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h, merge extractive liquid,, filter, get filtrate;
Step (three): the filtrate in the step (two) is concentrated, get concentrated solution, concentration is 0.20g/mL crude drug amount, bottling, sealing;
Step (four): the concentrated solution in the step (three) is carried out gastrointestinal protective effect test, and this Chinese medicine compound concentrated solution preparation has protective effect and the gastrointestinal motility function is had facilitation gastric mucosa.
Embodiment 2:
Step (one): select high-quality Radix Astragali 15g, Radix Ginseng 9g, Pericarpium Citri tangerinae 15g, Caulis Bambusae In Taenia 9g, Fructus Hordei Germinatus 15g, Fructus Crataegi 9g, Fructus Jujubae 6g and clean, microwave drying, pulverizing is 50 orders;
Step (two): the powder in the step () is added water boil extract 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, for the second time add 4 times of water gaging heated and boiled and carry 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, get filtrate;
Step (three): the filtrate in the step (two) is concentrated, get concentrated solution, concentration is 0.20g/mL crude drug amount, bottling, sealing;
Step (four): the concentrated solution in the step (three) is carried out gastrointestinal protective effect test, and this Chinese medicine compound concentrated solution preparation has protective effect and the gastrointestinal motility function is had facilitation gastric mucosa.
Chinese medicine compound concentrated solution preparation has protective effect to gastric mucosa and the gastrointestinal motility function is had the test of facilitation
1 material and instrument
1.1 tried thing and medicine
The preparation of compound Chinese medicinal preparation: Chinese medicine compound Radix Astragali 6g, Radix Ginseng 3g, Pericarpium Citri tangerinae 3g, Caulis Bambusae In Taenia 3g, Fructus Hordei Germinatus 3g, Fructus Crataegi 3g, Fructus Jujubae 3g powder add water boil and extract 3 times, and filtrate concentrates, and get concentrated solution, and concentration is 0.20g/mL crude drug amount; Ranitidine (the sharp prosperous pharmaceutical Co. Ltd in Shijiazhuang, lot number: 20081202); Domperidone (Xian-Janssen Pharmaceutical Ltd., lot number: 050915216); (bio-engineering research institute, lot number: 20091111) are built up in Nanjing for SOD (superoxide dismutase) and MDA (malonaldehyde) test kit.
1.2 animal
Cleaning level Kunming mouse, Shandong Luye Pharmaceutical Co., Ltd. provides, body weight 18-22g, male and female dual-purpose.Ambient temperature: 22 ± 2 ℃, the animal indoor feeding of 12h periodicity of illumination is freely drunk water, is taken food.Raised for 1 week before the experiment to conform.
1.3 instrument
RM6240 multi-channel signal instrument (Chengdu Instruement Factory), SC-3610 low speed centrifuge (Keda Innovation Co., Ltd), 752 type ultraviolet-uisible spectrophotometers (Shanghai Spectrum Apparatus Co., Ltd.), DY89-II tissue refiner (NingBo XinZhi Biology Science Co., Ltd).
2 methods
2.1 gastric injury experiment
2.1.1 animal grouping and modeling
Get 50 of 18-22g Kunming mouses, the male and female dual-purpose is divided into 5 groups at random, 10 every group.The basic, normal, high dosage group of compound Chinese medicinal preparation: difference ig 1925mgkg
-1, 3850mgkg
-1, 7700mgkg
-1Compound Chinese medicinal preparation; Ranitidine group ig 550mgkg
-1Normal control group, ethanol damage matched group: give ig distilled water by administration group equal-volume, continuously 5d.
2.1.2 the calculating of ulcer index and suppression ratio
Press the described method administration of 2.2.1, next day after the last administration, (d6) mice freely drank water, the cervical vertebra dislocation is put to death behind the fasting 24h except that the normal control group, it is dirty to open abdominal cavity clip stomach, open gastral cavity along greater gastric curvature and observe gastric mucosa, calculate ulcer index (UI) and suppression ratio, by the score of ulcer area size: measure the maximum major diameter of ulcer surface and perpendicular to the maximum wide footpath of maximum major diameter, the product of the two is ulcer index (UI) with rule.Ulcer inhibition rate/%=(ethanol damage group ulcer index-administration group ulcer index)/ethanol damage group ulcer index * 100.
2.1.3SOD, MDA measures
Get the gastric tissue piece, carry out SOD and MDA mensuration according to the method for test kit.
2.2 intestinal propulsion experiment
2.2.1 animal grouping and modeling
Get the 18-22g Kunming mouse, the male and female dual-purpose is divided into 5 groups at random, 10 every group.The basic, normal, high dosage group of compound Chinese medicinal preparation is pressed 1925mgkg respectively
-1, 3850mgkg
-1, 7700mgkg
-1Administration.Domperidone group: domperidone 7.33mgkg
-1Normal control group: give distilled water by administration group equal-volume.The equal per os gastric infusion of above medicine, successive administration 7 days.
2.2.2 the mensuration of intestinal propulsion rate
Next day after the last administration, (d8) mice freely drank water, behind the fasting 24h, behind each dosage group difference administration or distilled water 30min, each dosage group per os is irritated stomach and is given 0.2mL india ink, at interval 20min, taking off cervical vertebra execution also cuts open the belly immediately, with digestive tract from pylorus to the complete extraction of rectum end, do not add traction, the tiling digestive tract, survey forward position, charcoal end to the pylorus distance, it is the intestinal propulsion rate that this length accounts for small intestinal total length percentage ratio.
2.3 statistical analysis
Adopt the SPSS17.0 statistical analysis software to carry out the t check, data have statistical significance with " mean ± standard deviation " expression, two-sided test with P＜0.05 expression difference.
3 results
3.1 treatment protective effect to mice ethanol gastric ulcer
With normal control group ratio, the ulcer index of ethanol damage group mice extremely significantly raise (P＜0.001); Compare with ethanol damage group,
The ulcer index of high, medium and low dosage group of compound Chinese medicinal preparation and ranitidine group all significantly reduces (P＜0.01), the results are shown in Table 1.
Table 1 compound Chinese medicinal preparation causes the influence of the ulcer index of gastric mucosa damage mice to ethanol
### and normal control group be than P＜0.001, and * * and ethanol damage group are than P＜0.01; N=10
3.2 influence to ethanol gastric ulcer mice gastric tissue SOD, MDA
With the normal control group relatively, the active no significant change (P＞0.05) of the SOD of ethanol damage group mice, MDA activity extremely significantly raise (P＜0.001); Compare with ethanol damage group, the SOD activity of (P＜0.01), high (P＜0.05) dosage group all significantly raises in the compound Chinese medicinal preparation, and the active significantly reduction (P＜0.01) of MDA the results are shown in Table 2.
Table 2 compound Chinese medicinal preparation causes the influence of gastric mucosa damage mice gastric tissue SOD, MDA to ethanol
### and normal control group be than P＜0.001, and * and ethanol damage group be than P＜0.05, and * * and ethanol damage group are than P＜0.01; N=10
3.3 the compound Chinese medicinal preparation Chinese medicine compound is to the influence of intestinal propulsion function
Compare with the normal control group, domperidone and compound Chinese medicinal preparation all can extremely significantly promote intestinal propulsion (P＜0.001); And dosage (3850mgkg in the compound Chinese medicinal preparation
-1) organize and compare with the domperidone group, can promote intestinal propulsion function (P＜0.05) significantly, the results are shown in Table 3.
Table 3 compound Chinese medicinal preparation is to the propulsive influence of mouse small intestine
* * and normal control group be than P＜0.001, and # and domperidone group are than P＜0.05; N=10
4 discuss
This test shows that compound Chinese medicinal preparation has protective effect and the gastrointestinal motility function is had facilitation gastric mucosa.
Claims (2)
1. one kind has the compound Chinese medicinal preparation of protective effect to gastrointestinal, it is characterized in that said preparation comprises following process:
Step (one): select high-quality Radix Astragali 6g-15g, Radix Ginseng 3g-9g, Pericarpium Citri tangerinae 3g-15g, Caulis Bambusae In Taenia 3g-9g, Fructus Hordei Germinatus 3g-15g, Fructus Crataegi 3g-9g, Fructus Jujubae 3g-6g and clean, microwave drying, pulverizing is 50 orders;
Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0-6.0h, heated and boiled is extracted 2.0-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0-1.5h, merge extractive liquid, filters, and gets filtrate;
Step (three): the filtrate in the step (two) is concentrated, get concentrated solution, concentration is 0.20g/mL crude drug amount, bottling, sealing;
Step (four): the concentrated solution in the step (three) is carried out gastrointestinal protective effect test, and this Chinese medicine compound concentrated solution preparation has protective effect and the gastrointestinal motility function is had facilitation gastric mucosa.
2. a kind of compound Chinese medicinal preparation that gastrointestinal is had protective effect according to claim 1; it is characterized in that the Radix Astragali is the dry root of Radix Astragali Astragalus membranaceus (Fisch.) Bungede; Radix Ginseng is the dry root of Panax ginseng C.A.Mey.; Fructus Citri tangerinae is the dry mature skin of Citrus reticulate Banco; Caulis Bambusae In Taenia is the intermediate layer of grass bambusa breviflora Munro Bambusa tuldoides Munro stem, and Fructus Hordei Germinatus is that the mature fruit of grass Fructus Hordei Vulgaris Hordeum vulgare L. gets through the drying of germinateing; Fructus Crataegi is that dry mature fruit and the Fructus Jujubae of Crataegus pinnatifida Bge. is the mature fruit of Ziziphusjujuba Mill.var.inermis (Bunge) Rehd..
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| CN2010101748168A CN101983701B (en) | 2010-04-29 | 2010-04-29 | Traditional Chinese medicine compound preparation with protective effects on stomach and intestines |
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| CN101983701B true CN101983701B (en) | 2011-11-02 |
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| CN105747213A (en) * | 2016-02-25 | 2016-07-13 | 中国科学院西北高原生物研究所 | Cynomorium songaricum product capable of improving gastric motility and preparation process thereof |
| CN109805381A (en) * | 2017-11-16 | 2019-05-28 | 江西康宝医药生物科技有限公司 | Improve intestines and stomach function, immunoregulatory oral solution based on Chinese medicine |
| CN112156162B (en) * | 2020-10-30 | 2022-06-28 | 山西健硕食品药品研究院有限公司 | Medicine for protecting gastric mucosa and preparation method thereof |
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