CN101974521A - Molecular marker SIsv0372 in close linkage with foxtail millet herbicide resistant gene - Google Patents
Molecular marker SIsv0372 in close linkage with foxtail millet herbicide resistant gene Download PDFInfo
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- CN101974521A CN101974521A CN2010105533767A CN201010553376A CN101974521A CN 101974521 A CN101974521 A CN 101974521A CN 2010105533767 A CN2010105533767 A CN 2010105533767A CN 201010553376 A CN201010553376 A CN 201010553376A CN 101974521 A CN101974521 A CN 101974521A
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Abstract
The invention belongs to the field of molecular biology and relates to a molecular marker, in particular to a molecular marker in close linkage with a foxtail millet herbicide resistant gene, with a nucleotide sequence of the molecular marker shown as SEQID NO:1, or a DNA fragment containing the nucleotide sequence shown in the SEQ ID NO:1 in a foxtail millet genome. The invention also relates to primers of the molecular marker, application of the molecular marker in the positioning of the foxtail millet herbicide resistant gene or in foxtail millet inheritance breeding, a foxtail millet herbicide resistant gene positioning method, and a foxtail millet breeding method. The invention finds the molecular marker SIsv0372 which is in close linkage with the foxtail millet herbicide resistant gene, associates the foxtail millet genome DNA sequence and the foxtail millet herbicide resistant gene, and better benefits the establishment of a foxtail millet molecular marker assisted breeding system.
Description
Technical field
The invention belongs to biology field, relate to a kind of molecule marker, particularly, relate to a kind of and the closely linked molecule marker of millet anti-herbicide gene.The invention still further relates to primer, purposes, a kind of millet anti-herbicide gene localization method and a kind of millet breeding method of this molecule marker in millet anti-herbicide gene location or millet genetic breeding of this molecule marker.
Background technology
Millet (Setaria italica L.Beauv.) is the important food crop that originate from China, mainly at the north of China, occupies the status of outbalance in national food production and grain security.Millet is the diploid self pollination crop, and its genome is less, about 470Mb, and these characteristics make it be well suited for object as genome research.
Oneself becomes the important measures that current farmland is controlled weeds effectively, improved crop yield and quality to utilize the weedicide weeding.Yet because paddy field weed kind is many, quantity is big, produces the kind that lacks antiweed again at present, and the broad-spectrum herbicide weeding can not be used in the paddy field, and this becomes a great problem that obstacle millet produces.
At present, the structure of genetic linkage maps has become the important foundation of the assignment of genes gene mapping, map based cloning and genome structure and functional study.And molecule marker is the basis that makes up genetic linkage maps.Therefore, press for discovery and the closely linked molecule marker of millet anti-herbicide gene.
Summary of the invention
The inventor, provides a kind of and the closely linked molecule marker SIsv0372 of millet (Setaria italica L.Beauv.) anti-herbicide gene, and following invention is provided thus through a large amount of experiment and unremitting efforts according to whole genome sequence:
One aspect of the present invention relates to a kind of and the closely linked molecule marker SIsv 0372 of millet anti-herbicide gene, and its nucleotide sequence is shown in SEQ ID NO:1.Wherein add frame and partly be the design of primers section:
703bp
In the present invention, term " with the closely linked molecule marker of millet anti-herbicide gene " is meant such molecule marker, and in genetic linkage maps, the genetic linkage of this molecule marker and millet anti-herbicide gene distance is less than 2cM or less than 3cM; Perhaps millet greater than 400 samples in, contain this molecule marker in the sample of anti-Sethoxydin type or the anti-Sethoxydin type of part.Anti-Sethoxydin type or the anti-Sethoxydin type of part determine description referring to hereinafter.
In the present invention, particularly, described weedicide is a Sethoxydin.Sethoxydin is the strong inner sucting conduction type cauline leaf treatment agent of a kind of selectivity, can be absorbed rapidly by the cauline leaf of gramineous weeds, and be transmitted to top and internode meristematic tissue, and its cell fission is destroyed.Sethoxydin selectivity between Gramineae and dicotyledons is very high, to broad leaf crop safety, be widely used in removing gramineous weedss such as barnyard grass grass, wild avena sativa, Herba Setariae Viridis, lady's-grass, Herba Eleusines Indicae, amur foxtail, wild broomcorn millet, arm shape grass, rye grass, millet genus, downy brome, Bermuda grass, reed, wheatgrass, false Chinese sorghum, cogongrass.
The primer that also relates in one aspect to molecule marker of the present invention of the present invention, its nucleotide sequence is shown in SEQ ID NO:2 and SEQ ID NO:3:
SIsv0372F：CTCAATCATAGGAGCCTGGT(SEQ?ID?NO：2)
SIsv0372R：CACATCAGTGGCAGAGCTAG(SEQ?ID?NO：3)。
Molecule marker of the present invention also can be for containing the dna fragmentation of nucleotide sequence shown in the SEQ ID NO:1; The length of this dna fragmentation is suitable length, but is not particularly limited, for example, less than 10,000bp, less than 5,000bp,, 000bp,, 500bp,, 200bp,, 000bp or less than 800bp less than 1 less than 1 less than 1 less than 2.
In one embodiment of the invention, described molecule marker (dna fragmentation that contains nucleotide sequence shown in the SEQ ID NO:1) is the dna fragmentation of nucleotide sequence shown in the SEQ ID NO:1 in the millet genome, 5 ' the end of the SEQ ID NO:1 that is promptly comprised and/or the nucleotide sequence beyond the 3 ' end also are the sequences in the millet genome, preferably, be the 5 ' end of SEQID NO:1 in the millet genome and/or the upstream and downstream sequence of 3 ' end.It will be understood by those skilled in the art that as long as amplification or detect this molecule marker in the millet genomic dna, must detect or increase and obtain containing the sequence shown in the SEQ ID NO:1.The length of the 5 ' end of SEQ ID NO:1 and/or the upstream and downstream sequence of 3 ' end is suitable length, be not particularly limited, for example, the length that satisfies molecule marker is less than 10,000bp, less than 5,000bp, less than 2,000bp, less than 1,500bp, less than 1,200bp, less than 1,000bp or less than 800bp.
In one embodiment of the invention, 5 ' the end and/or 3 ' of the SEQ ID NO:1 that described molecule marker (dna fragmentation that contains nucleotide sequence shown in the SEQ ID NO:1) is comprised is held be operably connected artificial sequence and/or control sequence, for example promotor, enhanser, terminator, restriction enzyme site, primer sequence or the like.Wherein, term " operationally " is defined as a kind of following conformation in the present invention, in this conformation, control sequence for example promotor is suitably placed on the position of SEQ ID NO:1, so that this control sequence instructs the generation of SEQ ID NO:1 encoded polypeptides.
Another aspect of the present invention relates to a kind of recombinant vectors, and it contains molecule marker of the present invention.Described recombinant vectors can be expression vector or the cloning vector that is inserted with molecule marker of the present invention.Of the present inventionly also relate in one aspect to a kind of reconstitution cell, it contains this recombinant vectors.
The preparation method who also relates in one aspect to molecule marker of the present invention of the present invention, the genomic dna of millet that comprises the steps: to use anti-Sethoxydin type carries out pcr amplification as template with above-mentioned primer, and the amplified production that obtains promptly contains described molecule marker; Preferably, also comprise the step of pcr amplification product being carried out purifying.In one embodiment of the invention, the millet of described anti-Sethoxydin type is for opening No. 3, paddy No. 1, confused flour beetle or opening the anti-Sethoxydin type of F2 in generation that No. 3 selfings of confused flour beetle produce.
To those skilled in the art, be appreciated that also can the DNA chemosynthesis method obtain molecule marker of the present invention.
The detection method that also relates in one aspect to described molecule marker of the present invention comprises the steps:
(1) nucleotide sequence according to molecule marker of the present invention designs primer;
(2) genomic dna with detected millet increases as template;
(3) judge whether there is this molecule marker in the amplified production.
For example, can with the genomic dna of detected millet template, (SEQID NO:2 and SEQ ID NO:3) carries out pcr amplification with above-mentioned primer, obtains amplified production.The amplified production that obtains can be checked order or gel electrophoresis.
The purposes of molecule marker of the present invention in millet anti-herbicide gene location or detection that also relate in one aspect to of the present invention.
The localized method of a kind of millet anti-herbicide gene that also relates in one aspect to of the present invention comprises the step of using molecule marker of the present invention.
The purposes of molecule marker of the present invention in the millet assistant breeding that also relate in one aspect to of the present invention.
Of the present inventionly also relate in one aspect to a kind of millet auxiliary breeding means, comprise the step that detects molecule marker of the present invention.
Molecule marker of the present invention can be used in from now on the molecular mark, it will be appreciated by those skilled in the art that, such as by detect whether exist molecule marker of the present invention to screen millet antiweed is (for example, can reference, the purposes of dna molecular marker in wheat breeding for disease resistance, east, Gansu Province institute's journal (natural science edition), the 16th the 1st phase of volume of April in 2006, P65-69).Detect have molecule marker of the present invention for anti-Sethoxydin type or the anti-Sethoxydin type of part, do not have the not anti-Sethoxydin type that is of this molecule marker.Described detection can be the method that PCR detects, and particularly, can use above-mentioned molecule marker primer of the present invention.Described detection can also be undertaken by sequence measurement.This millet auxiliary breeding means has easy, quick, high-throughout advantage.
In the present invention, particularly, described millet can be for opening paddy No. 1, millet A2 sterile line, No. 3, a confused flour beetle or opening the F2 generation that No. 3 selfings of confused flour beetle produce.Wherein, opening paddy No. 1 or open the part F2 generation (the bud seedling leaf is normal) that No. 3 selfings of confused flour beetle produce is anti-Sethoxydin type; The part F2 generation (the bud seedling leaf fades) of opening No. 3, a confused flour beetle or No. 3 selfings of a confused flour beetle generation is the anti-Sethoxydin type of part; In the part F2 generation (the bud seedling leaf is withered) that millet A2 sterile line, No. 3 selfing of confused flour beetle produce, be not anti-Sethoxydin type.
Above-mentioned anti-Sethoxydin type, the anti-Sethoxydin type of part, not anti-Sethoxydin type are determined by following method:
Agar powder (agar powder) the substratum aqueous solution of configuration 4L 15g/L, the Autoclave sterilization is dissolved.Treat its temperature be cooled to about 50 ℃ the Sethoxydin that adds 6ml (available from Aladdin reagent (China) company limited, article No.: 1113709), fall plate, about the about 0.8cm of substratum thickness.After the substratum cooling, spread F2 for seed in substratum, place light training chamber to cultivate 3 days, statistics bud seedling leaf situation is normal, fades, and is still withered.Wherein, the bud seedling leaf be anti-Sethoxydin type normally, and what fade is the anti-Sethoxydin type of part, withered is not anti-Sethoxydin type.
The beneficial effect of the invention
The invention provides and the closely linked molecule marker of millet anti-herbicide gene, and millet genomic dna and millet anti-herbicide gene are connected, more help the foundation of millet molecular mark system; The hereditary close linkage distance of described molecule marker and anti-herbicide gene is 1.6cM.Molecule marker of the present invention can be applied to the millet assistant breeding easy, quick, high-throughput.
Description of drawings
Fig. 1: molecule marker primer (SEQ ID NO:2 and SEQ ID NO:3) is to the partial results of F2 generation 480 individual plant amplifications.Wherein:
Swimming lane 1-12 is the pcr amplification product of the individual plant of the anti-Sethoxydin type of 12 strains and the anti-Sethoxydin type of part in F2 generation; Swimming lane 14-25 is the pcr amplification product of F2 individual plant of the not anti-Sethoxydin type of 12 strains in generation.Swimming lane 13 is ma rker, and it is 200bp DNA Ladder; Its molecular weight comprises: 4000bp, 3000bp, 2500bp, 2000bp, 1800bp, 1600bp, 1400bp, 1200bp, 1000bp, 800bp, 600bp, 400bp and 200bp.The result shows: stripe size is 703bp in the plant of anti-Sethoxydin type and the anti-Sethoxydin type of part, and stripe size is less than 703bp in the plant of not anti-Sethoxydin type.
Explanation about the biomaterial preservation
The present invention relates to following biomaterial:
1. open No. 1 seed of paddy, it is preserved in Chinese typical culture collection center (CCTCC) on September 6th, 2010, and deposit number is CCTCC NO:P201012, and the preservation address is Luojiashan, Wuchang, Wuhan City, Hubei Province Wuhan University preservation center, and 430072.
2. millet A2 male-sterile seed, it is preserved in Chinese typical culture collection center (CCTCC) on September 6th, 2010, and deposit number is CCTCC NO:P201013, and the preservation address is Luojiashan, Wuchang, Wuhan City, Hubei Province Wuhan University preservation center, 430072.
3. open No. 3 seeds of confused flour beetle, it is preserved in Chinese typical culture collection center (CCTCC) on September 6th, 2010, and deposit number is CCTCC NO:P201010, and the preservation address is Luojiashan, Wuchang, Wuhan City, Hubei Province Wuhan University preservation center, and 430072.
Embodiment
Below in conjunction with embodiment embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example only is used to illustrate the present invention, and should not be considered as limiting scope of the present invention.Unreceipted actual conditions person among the embodiment carries out according to the condition of normal condition or manufacturers's suggestion.The unreceipted person of production firm of agents useful for same or instrument, being can be by the conventional products of commercial acquisition.
Embodiment 1: millet F2 is for the structure of segregating population
Male parent is for opening paddy No. 1: anti-Sethoxydin type (plant type height, plant height are about 150cm), and boot leaf is long and narrow, the bristle redness, clever shell redness can be educated, and the leaf colour cast is green.
Female parent is the A2 sterile line: not anti-Sethoxydin type (plant type is short, and plant height is about 100cm), boot leaf is short and wide, bristle green, clever shell green, partial sterility, leaf colour cast Huang.
Male parent and hybridization of female parent obtain F1 (plant height is about 130cm for No. 3, a confused flour beetle, the anti-Sethoxydin type of part).
F1 produces F2 for colony for selfing, obtains 480 individual plants altogether.480 F2 are carried out anti-Sethoxydin character analysis for individuality according to the method for aforementioned definite anti-Sethoxydin type, find the anti-Sethoxydin type of anti-Sethoxydin type and part totally 360 strains (wherein anti-Sethoxydin type 120 strains, anti-Sethoxydin type 240 strains of part), not anti-Sethoxydin type 120 strains.
Embodiment 2: in father and mother this and F1 generation, F2, are for the extraction of genes of individuals group DNA
With the CTAB method extract respectively among the embodiment 1 father and mother this, F1 generation and 480 F2 be for the genomic dna of individuality, concrete grammar is as follows:
(1) takes by weighing the fresh blade of 1.0g, shred and put into mortar,, grind to form homogenate and change in the centrifuge tube of 15ml, add 1ml 1.5 * CTAB flushing then in the mortar and change in the centrifuge tube again with adding 3ml1.5 * CTAB after the liquid nitrogen grinding.Behind the mixing in 65 ℃ of water-baths 30 minutes, during slowly shake up frequently.
1.5 * CTAB prescription following (1L) wherein:
CTAB 15g
(1MTris.C1 pH is 8.0) 75ml
0.5M?EDTA 30ml
NaCl 61.4g
Add deionized water and be settled to 1L, adding final concentration before using is the mercaptoethanol of 0.2% (2ml).
(2) to be cooled to room temperature, add equal-volume chloroform/primary isoamyl alcohol (24:1), mixing gently becomes deep green to subnatant.
(3) 4200rpm is centrifugal 10 minutes, and upper water is moved on to new 15ml centrifuge tube mutually, adds the dehydrated alcohol of 2 times of volume precoolings, mixes static 5 minutes.Place 30 minutes deposit D NA in-20 ℃.
(4) 4200rpm is centrifugal 10 minutes, discards supernatant, adds 1ml 75% washing with alcohol precipitation 1 time, is inverted the centrifuge tube dry DNA, adds 200 μ l TE dissolving DNAs.
(5) detect genomic dna with 0.8% sepharose.
(6) with the father and mother that obtain this and F1 generation, F2 for the genomic dna of individuality be stored in-20 ℃ standby.
Embodiment 3: the preparation of molecule marker
With the male parent, F1 generation or the F2 that extract among the embodiment 2 in generation anti-Sethoxydin type or the genomic dna of the anti-Sethoxydin type of part be template, (SEQ ID NO:2 and SEQ ID NO:3) carries out pcr amplification with the molecule marker primer.
The PCR reaction system is as follows:
Sterilized water 20.2 μ l
10*Buffer (contains Mg
2+) 2.5 μ l
dNTPs(25mM) 0.15μl
Taq enzyme (5U/ μ l) 0.15 μ l
Forward primer 0.5 μ l
Reverse primer 0.5 μ l
Template 1.0 μ l
Cumulative volume 25 μ l
The PCR response procedures is as follows:
94 ℃ of pre-sex change 5 minutes; 94 ℃ of sex change 30 seconds, 60 ℃ of annealing 30 seconds, 72 ℃ were extended 40 seconds, and moved 35 circulations; Last 72 ℃ were extended 3 minutes.P ℃ of R amplified production can be 4 ℃ of preservations.
With the amplified production purifying, obtain molecule marker.Check order behind the purifying, the result is shown in SEQ IDNO:1.
To those skilled in the art, be appreciated that also and can obtain this molecule marker by the method for DNA chemosynthesis.
Embodiment 4: the preliminary screening of molecule marker primer
Male parent is carried out de novo order-checking (60X contig N50:22K, scaffold N50:320K; Total size:400Mb), the female parent preface (10X) of resurveying.
According to this sequencing data of father and mother, utilize SOAP software (SOAP2.20 for example, can download from http://soap.genomics.org.cn/, also can use other sequence alignment software) sequence difference of father and mother between this relatively, then based on the sequence of difference, in male parent 5 ' end and about 50bp position, 3 ' the end outside, length about picked at random 20bp, design primer at random with primerpremier software, designed 1105 pairs of primers altogether, the sequence (SEQ ID NO:2-41) of part primer wherein has been shown in the following table 1.
Table 1:1105 is to the part primer in the random primer
Genomic dna with the father and mother that extract among the embodiment 2 this and F1 generation is a template respectively, carries out pcr amplification with 1105 pairs of primers of design, and is similar among other condition of PCR reaction system and program and the embodiment 3.
The PCR product is carried out agarose electrophoresis detect, to detect the validity and the polymorphism of primer.Be meant in this validity whether amplified production is arranged; Polymorphism is meant that the clip size of this amplified production of father and mother is variant.
Carry out the screening of primer according to following screening criteria: father and mother's basis and F1 all have amplified production, and this amplified production of father and mother all has only a distinct banding pattern and big or small variant, F1 shows as the heterozygosis banding pattern of this banding pattern of father and mother, and two bands of male parent and maternal banding pattern are promptly arranged.
The selection result: from 1105 pairs of primers of design at random, filter out 616 pairs of primers.
Embodiment 5: millet F2 is for the structure of genetic linkage maps
480 individualities with 616 pairs of molecule marker primers F 2 colonies that obtain among the embodiment 4 carry out the PCR detection respectively, the genomic dna of template used 480 individualities for the F2 colony that makes among the embodiment 2, other composition and the PCR program of PCR system are identical with embodiment 4.
The PCR product is carried out agarose gel electrophoresis, and wherein molecule marker primer (SEQ ID NO:2 and SEQ ID NO:3) to 480 individual partial results that increase as shown in Figure 1.
Whole electrophoresis result are carried out data statistic analysis, concrete grammar is as follows: be a that is designated as of male parent type with F2 colony individual plant amplified band, amplified band is the b that is designated as of maternal type, amplified band contains the h that is designated as of male parent type and maternal type simultaneously, all do not have be designated as-, finally obtain the genotype data of 616 pairs of primer amplifications of 480 individualities of F2 colony.Such as, the data of 480 individualities that obtain with first pair of primer are a, b, and h ,-, b ... totally 480 data, the data that obtain with second pair of primer are b, a, h, a ,-... totally 480 data, totally 616 pairs of primers are added up respectively, and gained is the genotype data of this F2 colony.
With MapMaker3.0 software (Constructing genetic maps withMAPMAKER/EXP 3.0, S Lincoln, M Daly, E Lander-Cambridge, MA:Whitehead Institute, 1992) carry out the genetic linkage maps drafting, obtain genetic linkage map.The genetic linkage map subscript understands that the position of 616 pairs of primers reaches and the genetic distance of anti-herbicide gene.
According to the antiweed phenotype of 480 individualities, similar to the male parent type proterties a (anti-Sethoxydin) that is designated as, similar to the maternal type proterties b (not anti-Sethoxydin) that is designated as, proterties occupy and is designated as h (the anti-Sethoxydin of part) between male parent and the female parent.Obtain the phenotypic data of 480 individualities, the phenotypic data of 480 individualities compares with the genotype data of 480 individualities that obtain before, and similar Gao Ze represents this mark and antiweed (the anti-Sethoxydin of anti-Sethoxydin or part) proterties close linkage.
According to The above results with antiweed (Sethoxydin) assignment of genes gene mapping on genetic linkage maps.
Embodiment 6: with the checking of the closely linked molecule marker of millet antiweed
1. on the basis of the genetic linkage maps that embodiment 5 makes, according to the genetic linkage distance of millet anti-herbicide gene, with the hereditary close linkage distance of millet anti-herbicide gene for having determined molecule marker primer (SEQ ID NO:2 and SEQ ID NO:3) in the position of 1.6cM, and find corresponding male parent sequence location, sequence between the upstream and downstream primer is molecule marker, and its nucleotide sequence is shown in SEQ ID NO:1.
2. in addition, in the electrophoresis in embodiment 5 to the pcr amplification product of 480 individualities in F2 generation, amplification for molecule marker primer (SEQ ID NO:2 and SEQ ID NO:3) is: the amplified production of 360 anti-Sethoxydin types and the anti-Sethoxydin type of part all has the band of 703bp size, and the pcr amplification product of 120 not anti-Sethoxydin types does not all have the band (the part amplification as shown in Figure 1) of 703bp.And the fragments sequence through this 703bp of order-checking proof is identical with SEQ ID NO:1.
As seen, molecule marker of the present invention (SEQ ID NO:1) is and the closely linked molecule marker of millet antiweed (Sethoxydin) gene.
Although the specific embodiment of the present invention has obtained detailed description, it will be understood to those of skill in the art that.According to disclosed all instructions, can carry out various modifications and replacement to those details, these change all within protection scope of the present invention.Four corner of the present invention is provided by claims and any equivalent thereof.
Claims (10)
- One kind with the closely linked molecule marker of millet anti-herbicide gene, its nucleotide sequence is shown in SEQ ID NO:1; Perhaps it is the dna fragmentation that contains nucleotide sequence shown in the SEQ ID NO:1.
- 2. recombinant vectors, it contains the described molecule marker of claim 1.
- 3. reconstitution cell, it contains the described recombinant vectors of claim 2.
- 4. the primer of the described molecule marker of claim 1, its nucleotide sequence is shown in SEQ IDNO:2 and SEQ ID NO:3.
- 5. the preparation method of the described molecule marker of claim 1 comprises the steps:The genomic dna of millet that uses anti-Sethoxydin type or the anti-Sethoxydin type of part carries out pcr amplification as template with the described primer of claim 4; Preferably, also comprise the step of the product of pcr amplification being carried out purifying; Particularly, the millet of described anti-Sethoxydin type is for opening paddy No. 1 or opening the anti-Sethoxydin type of F2 in generation that No. 3 selfings of confused flour beetle produce; The millet of the anti-Sethoxydin type of described part is for opening No. 3, confused flour beetle or opening the part anti-Sethoxydin type of F2 in generation that No. 3 selfings of confused flour beetle produce.
- 6. the detection method of the described molecule marker of claim 1 comprises the steps:(1) according to the nucleotide sequence design primer of the molecule marker of claim 1;(2) genomic dna with detected millet increases as template;(3) judge whether there is this molecule marker in the amplified production.
- 7. the described molecule marker of claim 1 is in millet anti-herbicide gene location or the purposes in detecting.
- 8. the localized method of millet anti-herbicide gene comprises the step of using the described molecule marker of claim 1.
- 9. the purposes of the described molecule marker of claim 1 in the millet assistant breeding.
- 10. a millet auxiliary breeding means comprises that test right requires the step of 1 described molecule marker.
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| CN102154285A (en) * | 2011-03-24 | 2011-08-17 | 深圳华大基因科技有限公司 | Molecular marker SIsv0737 closely linked with millet leaf color gene |
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| CN102690816A (en) * | 2011-03-24 | 2012-09-26 | 深圳华大基因科技有限公司 | Molecular marker SIsv1241 closely linked with Setaria italica L. Beauv. pollen color gene |
| CN102690819A (en) * | 2011-03-24 | 2012-09-26 | 深圳华大基因科技有限公司 | Molecular marker SIsv0553 closely linked with Setaria italica L. Beauv. pollen color gene |
| CN102690813A (en) * | 2011-03-24 | 2012-09-26 | 深圳华大基因科技有限公司 | Molecular marker SIsv0701 closely linked with Setaria italica L. Beauv. seta color gene |
| CN102690810A (en) * | 2011-03-24 | 2012-09-26 | 深圳华大基因科技有限公司 | Molecular marker SIsv0690 closely linked with Setaria italica L. Beauv. leaf color gene |
| CN102690812B (en) * | 2011-03-24 | 2016-06-01 | 深圳华大基因科技有限公司 | Molecular marker SIsv0067 closely linked with millet Heading date gene |
| CN102690811B (en) * | 2011-03-24 | 2016-06-01 | 深圳华大基因科技有限公司 | Molecular marker SIsv1363 closely linked with millet leaf color gene |
| CN107385105A (en) * | 2017-09-22 | 2017-11-24 | 山西省农业科学院谷子研究所 | With molecular labeling, primer and the application of millet glume color trait close linkage |
| CN108660233A (en) * | 2017-12-08 | 2018-10-16 | 深圳华大小米产业股份有限公司 | A kind of molecular labeling SIsv0456 with millet anti-imazethapyr herbicide resistance gene close linkage |
| CN108660231A (en) * | 2017-12-08 | 2018-10-16 | 深圳华大小米产业股份有限公司 | With the molecular labeling SIsv1208 of the anti-Sethoxydin herbicide resistance gene close linkage of millet |
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| CN102154285A (en) * | 2011-03-24 | 2011-08-17 | 深圳华大基因科技有限公司 | Molecular marker SIsv0737 closely linked with millet leaf color gene |
| CN102154282A (en) * | 2011-03-24 | 2011-08-17 | 深圳华大基因科技有限公司 | Molecular marker SIsv0151 closely interlocked with gene associated with leaf color of millet |
| CN102154281A (en) * | 2011-03-24 | 2011-08-17 | 深圳华大基因科技有限公司 | Molecular marker SIsv0010 closely linked with heading-date gene of millet |
| CN102154281B (en) * | 2011-03-24 | 2012-09-19 | 深圳华大基因科技有限公司 | Molecular marker SIsv0010 closely linked with heading-date gene of millet |
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| CN102690817A (en) * | 2011-03-24 | 2012-09-26 | 深圳华大基因科技有限公司 | Molecular marker SIsv0659 closely linked with Setaria italica L. Beauv. pollen color gene |
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| CN102690819A (en) * | 2011-03-24 | 2012-09-26 | 深圳华大基因科技有限公司 | Molecular marker SIsv0553 closely linked with Setaria italica L. Beauv. pollen color gene |
| CN102690813A (en) * | 2011-03-24 | 2012-09-26 | 深圳华大基因科技有限公司 | Molecular marker SIsv0701 closely linked with Setaria italica L. Beauv. seta color gene |
| CN102690810A (en) * | 2011-03-24 | 2012-09-26 | 深圳华大基因科技有限公司 | Molecular marker SIsv0690 closely linked with Setaria italica L. Beauv. leaf color gene |
| CN102154282B (en) * | 2011-03-24 | 2012-11-14 | 深圳华大基因科技有限公司 | Molecular marker SIsv0151 closely interlocked with gene associated with leaf color of millet |
| CN102690819B (en) * | 2011-03-24 | 2015-11-25 | 深圳华大基因科技有限公司 | Molecule marker SIsv0553 closely linked with millet pollen color gene |
| CN102690815B (en) * | 2011-03-24 | 2015-12-02 | 深圳华大基因科技有限公司 | Molecule marker SIsv0235 closely linked with millet bristle color gene |
| CN102690813B (en) * | 2011-03-24 | 2016-01-13 | 深圳华大基因科技有限公司 | Molecule marker SIsv0701 closely linked with millet bristle color gene |
| CN102690814B (en) * | 2011-03-24 | 2016-03-30 | 深圳华大基因科技有限公司 | Molecule marker SIsv0491 closely linked with millet bristle color gene |
| CN102690816B (en) * | 2011-03-24 | 2016-04-20 | 深圳华大基因科技有限公司 | Molecule marker SIsv1241 closely linked with millet pollen color gene |
| CN102154285B (en) * | 2011-03-24 | 2016-04-27 | 深圳华大基因科技有限公司 | Molecule marker SIsv0737 closely linked with millet leaf color gene |
| CN102690817B (en) * | 2011-03-24 | 2016-05-04 | 深圳华大基因科技有限公司 | With the closely linked molecular labeling SIsv0659 of millet pollen color gene |
| CN102690810B (en) * | 2011-03-24 | 2016-06-01 | 深圳华大基因科技有限公司 | Molecular marker SIsv0690 closely linked with millet leaf color gene |
| CN102690812B (en) * | 2011-03-24 | 2016-06-01 | 深圳华大基因科技有限公司 | Molecular marker SIsv0067 closely linked with millet Heading date gene |
| CN102690818B (en) * | 2011-03-24 | 2016-06-01 | 深圳华大基因科技有限公司 | Molecular marker SIsv0832 closely linked with millet Heading date gene |
| CN102690811B (en) * | 2011-03-24 | 2016-06-01 | 深圳华大基因科技有限公司 | Molecular marker SIsv1363 closely linked with millet leaf color gene |
| CN107385105A (en) * | 2017-09-22 | 2017-11-24 | 山西省农业科学院谷子研究所 | With molecular labeling, primer and the application of millet glume color trait close linkage |
| CN108660233A (en) * | 2017-12-08 | 2018-10-16 | 深圳华大小米产业股份有限公司 | A kind of molecular labeling SIsv0456 with millet anti-imazethapyr herbicide resistance gene close linkage |
| CN108660231A (en) * | 2017-12-08 | 2018-10-16 | 深圳华大小米产业股份有限公司 | With the molecular labeling SIsv1208 of the anti-Sethoxydin herbicide resistance gene close linkage of millet |
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