CN101985620B - Molecular marker SIsv1223 closely linked with millet herbicide-resistant gene - Google Patents
Molecular marker SIsv1223 closely linked with millet herbicide-resistant gene Download PDFInfo
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- CN101985620B CN101985620B CN2010105533593A CN201010553359A CN101985620B CN 101985620 B CN101985620 B CN 101985620B CN 2010105533593 A CN2010105533593 A CN 2010105533593A CN 201010553359 A CN201010553359 A CN 201010553359A CN 101985620 B CN101985620 B CN 101985620B
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Abstract
The invention belongs to the field of molecular biology and relates to a molecular marker, in particular to a molecular marker closely linked with a millet herbicide-resistant gene. The nucleotide sequence of the molecular marker is shown as SEQID NO:1, or the molecular marker is a deoxyribonucleic acid (DNA) segment which contains the nucleotide sequence shown as SEQ ID NO:1 in a millet genome. The invention also relates to a primer of the molecular marker, the application of the molecular marker to millet herbicide-resistant gene positioning or millet genetic breeding, a millet herbicide-resistant gene positioning method and a millet breeding method. In the invention, a molecular marker SIsv1223 closely linked with the millet herbicide-resistant gene is found, so that a millet genome DNA sequence and the millet herbicide-resistant gene are linked with each other and the establishment of a millet molecular marker-assisted breeding system is further facilitated.
Description
Technical field
The invention belongs to biology field, relate to a kind of molecule marker, particularly, relate to a kind of and the closely linked molecule marker of millet anti-herbicide gene.The invention still further relates to primer, purposes, a kind of millet anti-herbicide gene localization method and a kind of millet breeding method of this molecule marker in millet anti-herbicide gene location or millet genetic breeding of this molecule marker.
Background technology
Millet (Setaria italica L.Beauv.) is the important food crop that originate from China, mainly at the north of China, in national food production and grain security, occupies the status of outbalance.Millet is the diploid self pollination crop, and its genome is less, about 470Mb, and these characteristics make it be well suited for the object as genome research.
Oneself becomes the important measures that current farmland is controlled weeds effectively, improved crop yield and quality to utilize the weedicide weeding.Yet because paddy field weed kind is many, quantity is big, produces the kind that lacks antiweed again at present, and the broad-spectrum herbicide weeding can not be used in the paddy field, and this becomes a great problem that obstacle millet produces.
At present, the structure of genetic linkage maps has become the important foundation of the assignment of genes gene mapping, map based cloning and genome structure and functional study.And molecule marker is the basis that makes up genetic linkage maps.Therefore, press for discovery and the closely linked molecule marker of millet anti-herbicide gene.
Summary of the invention
The inventor, provides a kind of and the closely linked molecule marker SIsv1223 of millet (Setaria italica L.Beauv.) anti-herbicide gene, and following invention is provided thus through a large amount of experiment and unremitting efforts according to whole genome sequence:
One aspect of the present invention relates to a kind of and the closely linked molecule marker SIsv1223 of millet anti-herbicide gene, and its nucleotide sequence is shown in SEQ ID NO:1.Wherein add frame and partly be the design of primers section:
553bp
In the present invention, term " with the closely linked molecule marker of millet anti-herbicide gene " is meant such molecule marker, and in genetic linkage maps, the genetic linkage of this molecule marker and millet anti-herbicide gene distance is less than 2cM or less than 3cM; Perhaps millet greater than 400 samples in, contain this molecule marker in the sample of anti-Sethoxydin type or the anti-Sethoxydin type of part.Confirming of anti-Sethoxydin type or the anti-Sethoxydin type of part referring to the description in the literary composition of back.
In the present invention, particularly, said weedicide is a Sethoxydin.Sethoxydin is the strong inner sucting conduction type cauline leaf treatment agent of a kind of selectivity, can be absorbed rapidly by the cauline leaf of gramineous weeds, and be transmitted to top and internode meristematic tissue, and its cell fission is destroyed.Sethoxydin selectivity between Gramineae and dicotyledons is very high; To broad leaf crop safety, be widely used in removing gramineous weedss such as barnyard grass grass, ild avena sativa, Herba Setariae Viridis, lady's-grass, Herba Eleusines Indicae, amur foxtail, wild broomcorn millet, arm shape grass, rye grass, millet genus, downy brome, Bermuda grass, reed, wheatgrass, false Chinese sorghum, cogongrass.
The primer that also relates in one aspect to molecule marker of the present invention of the present invention, its nucleotide sequence is shown in SEQ ID NO:2 and SEQ ID NO:3:
SIsv1223F：TCAGTTTGCAGACCTGGACT(SEQ?ID?NO：2)
SIsv1223R：CAGAAGGAACCAAAGGCAGT(SEQ?ID?NO：3)。
Molecule marker of the present invention also can be for containing the dna fragmentation of nucleotide sequence shown in the SEQ ID NO:1; The length of this dna fragmentation is suitable length, but is not particularly limited, for example, less than 10,000bp, less than 5,000bp,, 000bp,, 500bp,, 200bp,, 000bp, or less than 800bp less than 1 less than 1 less than 1 less than 2.
In one embodiment of the invention; Said molecule marker (dna fragmentation that contains nucleotide sequence shown in the SEQ ID NO:1) is the dna fragmentation of nucleotide sequence shown in the SEQ ID NO:1 in the millet genome; 5 ' end and/or the nucleotide sequence beyond the 3 ' end of the SEQ ID NO:1 that is promptly comprised also are the sequences in the millet genome; Preferably, be the 5 ' end of SEQID NO:1 in the millet genome and/or the upstream and downstream sequence of 3 ' end.It will be understood by those skilled in the art that needing only amplification perhaps detects this molecule marker in the millet genomic dna, must detect or increase to obtain containing the sequence shown in the SEQ ID NO:1.The length of the 5 ' end of SEQ ID NO:1 and/or the upstream and downstream sequence of 3 ' end is suitable length, is not particularly limited, for example; The length that satisfies molecule marker is less than 10,000bp, less than 5,000bp, less than 2; 000bp, less than 1; 500bp, less than 1,200bp, less than 1,000bp, or less than 800bp.
In one embodiment of the invention; 5 ' the end and/or 3 ' of the SEQ ID NO:1 that said molecule marker (dna fragmentation that contains nucleotide sequence shown in the SEQ ID NO:1) is comprised is held be operably connected artificial sequence and/or control sequence, for example promotor, enhanser, terminator, restriction enzyme site, primer sequence or the like.Wherein, Term " operationally " is defined as a kind of following conformation in the present invention; In this conformation, control sequence for example promotor is suitably placed on the position of SEQ ID NO:1, so that this control sequence instructs the generation of SEQ ID NO:1 encoded polypeptides.
Another aspect of the present invention relates to a kind of recombinant vectors, and it contains molecule marker of the present invention.Said recombinant vectors can be expression vector or the cloning vector that is inserted with molecule marker of the present invention.Of the present inventionly also relate in one aspect to a kind of reconstitution cell, it contains this recombinant vectors.
The preparation method who also relates in one aspect to molecule marker of the present invention of the present invention; The genomic dna of millet that comprises the steps: to use anti-Sethoxydin type or the anti-Sethoxydin type of part is as template; Carry out pcr amplification with above-mentioned primer, the amplified production that obtains promptly contains said molecule marker; Preferably, also comprise the step of pcr amplification product being carried out purifying.
To those skilled in the art, be appreciated that also can the DNA chemosynthesis method obtain molecule marker of the present invention.
The detection method that also relates in one aspect to said molecule marker of the present invention comprises the steps:
(1) nucleotide sequence according to molecule marker of the present invention designs primer;
(2) genomic dna with millet to be detected increases as template;
(3) judge whether there is this molecule marker in the amplified production.
For example, can with the genomic dna of millet to be detected template, (SEQID NO:2 and SEQ ID NO:3) carries out pcr amplification with above-mentioned primer, obtains amplified production.Can the amplified production that obtain be checked order or gel electrophoresis.
The purposes of molecule marker of the present invention in millet anti-herbicide gene location or detection that also relate in one aspect to of the present invention.
The localized method of a kind of millet anti-herbicide gene that also relates in one aspect to of the present invention comprises the step of using molecule marker of the present invention.
The purposes of molecule marker of the present invention in the millet assistant breeding that also relate in one aspect to of the present invention.
Of the present inventionly also relate in one aspect to a kind of millet auxiliary breeding means, comprise the step that detects molecule marker of the present invention.
Molecule marker of the present invention can be used in the molecular mark from now on; It will be understood by those skilled in the art that such as through detect whether exist molecule marker of the present invention to screen millet antiweed (for example, can reference; The purposes of dna molecular marker in wheat breeding for disease resistance; East, Gansu Province institute's journal (natural science edition), the 16th the 1st phase of volume of April in 2006, P65-69).Detect have molecule marker of the present invention for anti-Sethoxydin type or the anti-Sethoxydin type of part, do not have the not anti-Sethoxydin type that is of this molecule marker.Said detection can be the method that PCR detects, and particularly, can use above-mentioned molecule marker primer of the present invention.Said detection can also be carried out through sequence measurement.This millet auxiliary breeding means has easy, quick, high-throughout advantage.
In the present invention, particularly, said millet can be for opening paddy No. 1, millet A2 sterile line, No. 3, a confused flour beetle or opening the F2 generation that No. 3 selfings of confused flour beetle produce.Wherein, opening paddy No. 1 or open the part F2 generation (the bud seedling leaf is normal) that No. 3 selfings of confused flour beetle produce is anti-Sethoxydin type; The part F2 generation (the bud seedling leaf fades) of opening No. 3, a confused flour beetle or No. 3 selfings of a confused flour beetle generation is the anti-Sethoxydin type of part; In the part F2 generation (the bud seedling leaf is withered) that millet A2 sterile line, No. 3 selfing of confused flour beetle produce, be not anti-Sethoxydin type.
Above-mentioned anti-Sethoxydin type, the anti-Sethoxydin type of part, not anti-Sethoxydin type are confirmed through following method:
Agar powder (agar powder) the substratum aqueous solution of configuration 4L 15g/L, the Autoclave sterilization is dissolved.Treat its temperature be cooled to about 50 ℃ the Sethoxydin that adds 6ml (available from Aladdin reagent (China) ltd, article No.: 1113709), fall plate, about the about 0.8cm of substratum thickness.After the substratum cooling, spread F2 for seed in substratum, place light training chamber to cultivate 3 days, statistics bud seedling leaf situation is normal, fades, and is still withered.Wherein, the bud seedling leaf be anti-Sethoxydin type normally, and what fade is the anti-Sethoxydin type of part, withered is not anti-Sethoxydin type.
The beneficial effect of the invention
The invention provides and the closely linked molecule marker of millet anti-herbicide gene, and millet genomic dna and millet anti-herbicide gene are connected, more help the foundation of millet molecular mark system; The hereditary close linkage distance of said molecule marker and anti-herbicide gene is 0.5cM.Molecule marker of the present invention can be applied to the millet assistant breeding easy, quick, high-throughput.
Description of drawings
Fig. 1: molecule marker primer (SEQ ID NO:2 and SEQ ID NO:3) is to the partial results of F2 generation 480 individual plant amplifications.Wherein:
Swimming lane 1-12 is the pcr amplification product of the individual plant of the anti-Sethoxydin type of 12 strains and the anti-Sethoxydin type of part in F2 generation; Swimming lane 14-25 is the pcr amplification product of F2 individual plant of the not anti-Sethoxydin type of 12 strains in generation.Swimming lane 13 is marker, and it is 200bp DNA Ladder; Its molecular weight comprises: 4000bp, 3000bp, 2500bp, 2000bp, 1800bp, 1600bp, 1400bp, 1200bp, 1000bp, 800bp, 600bp, 400bp and 200bp.The result shows: stripe size is 553bp in the plant of anti-Sethoxydin type and the anti-Sethoxydin type of part, and stripe size is less than 553bp in the plant of not anti-Sethoxydin type.
Explanation about the biomaterial preservation
The present invention relates to following biomaterial:
1. open No. 1 seed of paddy, it is preserved in Chinese typical culture collection center (CCTCC) on September 6th, 2010, and deposit number is CCTCC NO:P201012, and the preservation address is Luojiashan, Wuchang, Wuhan City, Hubei Province Wuhan University preservation center, and 430072.
2. millet A2 male-sterile seed, it is preserved in Chinese typical culture collection center (CCTCC) on September 6th, 2010, and deposit number is CCTCC NO:P201013, and the preservation address is Luojiashan, Wuchang, Wuhan City, Hubei Province Wuhan University preservation center, 430072.
3. open No. 3 seeds of confused flour beetle, it is preserved in Chinese typical culture collection center (CCTCC) on September 6th, 2010, and deposit number is CCTCC NO:P201010, and the preservation address is Luojiashan, Wuchang, Wuhan City, Hubei Province Wuhan University preservation center, and 430072.
Embodiment
To combine embodiment that embodiment of the present invention are described in detail below, but it will be understood to those of skill in the art that the following example only is used to explain the present invention, and should not be regarded as limiting scope of the present invention.Unreceipted actual conditions person among the embodiment carries out according to the condition of normal condition or manufacturers's suggestion.The unreceipted person of production firm of agents useful for same or instrument, being can be through the conventional products of commercial acquisition.
Embodiment 1: millet F2 is for the structure of segregating population
Male parent is for opening paddy No. 1: anti-Sethoxydin type (plant type is high, and plant height is about 150cm), and boot leaf is long and narrow, and bristle is red, and clever shell is red, can educate, and the leaf colour cast is green.
Female parent is the A2 sterile line: not anti-Sethoxydin type (plant type is short, and plant height is about 100cm), and boot leaf is short and wide, and bristle is green, and clever shell is green, partial sterility, the leaf colour cast is yellow.
Male parent and hybridization of female parent obtain F1 (plant height is about 130cm for No. 3, a confused flour beetle, the anti-Sethoxydin type of part).
F1 produces F2 for colony for selfing, obtains 480 individual plants altogether.480 F2 are carried out anti-Sethoxydin character analysis for individuality according to the method for aforementioned definite anti-Sethoxydin type; Find the anti-Sethoxydin type of anti-Sethoxydin type and part totally 360 strains (wherein anti-Sethoxydin type 120 strains; Anti-Sethoxydin type 240 strains of part), not anti-Sethoxydin type 120 strains.
Embodiment 2: in father and mother this and F1 generation, F2, are for the extraction of genes of individuals group DNA
With the CTAB method extract respectively among the embodiment 1 father and mother this, F1 generation and 480 F2 be for the genomic dna of individuality, concrete grammar is following:
(1) takes by weighing the fresh blade of 1.0g, shred and put into mortar,, grind to form homogenate and change in the centrifuge tube of 15ml, add 1ml 1.5 * CTAB flushing then in the mortar and change in the centrifuge tube again with adding 3ml1.5 * CTAB after the liquid nitrogen grinding.Behind the mixing in 65 ℃ of water-baths 30 minutes, during slowly shake up frequently.
1.5 * CTAB prescription (1L) as follows wherein:
CTAB 15g
1M Tris.Cl (pH is 8.0) 75ml
0.5M?EDTA 30ml
NaCl 61.4g
Add deionized water and be settled to 1L, adding final concentration before using is the mercaptoethanol of 0.2% (2ml).
(2) to be cooled to room temperature, add equal-volume chloroform/primary isoamyl alcohol (24: 1), mixing gently becomes deep green to subnatant.
(3) 4200rpm is centrifugal 10 minutes, and upper water is moved on to new 15ml centrifuge tube mutually, adds the absolute ethyl alcohol of 2 times of volume precoolings, mixes static 5 minutes.Place 30 minutes deposit D NA in-20 ℃.
(4) 4200rpm is centrifugal 10 minutes, discards supernatant, adds 1ml 75% washing with alcohol deposition 1 time, is inverted the centrifuge tube dry DNA, adds 200 μ l TE dissolving DNAs.
(5) detect genomic dna with 0.8% sepharose.
(6) with the father and mother that obtain this and F1 generation, F2 for the genomic dna of individuality be stored in-20 ℃ subsequent use.
Embodiment 3: the preparation of molecule marker
With the male parent, F1 generation or the F2 that extract among the embodiment 2 anti-Sethoxydin type or the genomic dna of the anti-Sethoxydin type of part in generation is template, and (SEQ ID NO:2 and SEQ ID NO:3) carries out pcr amplification with the molecule marker primer.
The PCR reaction system is following:
Sterilized water 20.2 μ l
10*Buffer (contains Mg
2+) 2.5 μ l
dNTPs(25mM) 0.15μl
Taq enzyme (5U/ μ l) 0.15 μ l
Forward primer 0.5 μ l
Reverse primer 0.5 μ l
Template 1.0 μ l
TV 25 μ l
The PCR response procedures is following:
94 ℃ of preparatory sex change 5 minutes; 94 ℃ of sex change 30 seconds, 60 ℃ of annealing 30 seconds, 72 extended 40 seconds, moved 35 circulations; Last 72 ℃ were extended 3 minutes.Pcr amplification product can be 4 ℃ of preservations.
With the amplified production purifying, obtain molecule marker.Check order behind the purifying, the result is shown in SEQ IDNO:1.
To those skilled in the art, be appreciated that also and can obtain this molecule marker through the method for DNA chemosynthesis.
Embodiment 4: the preliminary screening of molecule marker primer
Male parent is carried out de novo order-checking (60X contig N50:22K, scaffold N50:320K; Total size:400Mb), the female parent preface (10X) of resurveying.
According to this sequencing data of father and mother; Utilize the sequence difference between SOAP software (for example SOAP2.20 can download from http://soap.genomics.org.cn/, also can use other sequence alignment software) the comparison father and mother basis; Then based on the sequence of difference; In male parent 5 ' end and about 50bp position, 3 ' the end outside, the length about picked at random 20bp designs primer at random with primerpremier software; Designed 1105 pairs of primers altogether, the sequence (SEQ ID NO:2-41) of part primer wherein has been shown in the following table 1.
Table 1:1105 is to the part primer in the random primer
Genomic dna with the father and mother that extract among the embodiment 2 this and F1 generation is a template respectively, carries out pcr amplification with 1105 pairs of primers of design, and is similar among other condition of PCR reaction system and program and the embodiment 3.
The PCR product is carried out agarose electrophoresis detect, to detect the validity and the polymorphum of primer.Be meant in this validity whether amplified production is arranged; Polymorphum is meant that the clip size of this amplified production of father and mother is variant.
Carry out the screening of primer according to following screening criteria: father and mother's basis and F1 all have amplified production; And this amplified production of father and mother all has only a distinct banding pattern and big or small variant; F1 shows as the heterozygosis banding pattern of this banding pattern of father and mother, and two bands of male parent and maternal banding pattern are promptly arranged.
The selection result: from 1105 pairs of primers of design at random, filter out 616 pairs of primers.
Embodiment 5: millet F2 is for the structure of genetic linkage maps
Carrying out PCR respectively with 480 individuals of 616 pairs of molecule marker primers F 2 colonies that obtain among the embodiment 4 detects; The genomic dna of template used 480 individuals for the F2 colony that makes among the embodiment 2, other composition and the PCR program of PCR system are identical with embodiment 4.
The PCR product is carried out agarose gel electrophoresis, and wherein molecule marker primer (SEQ ID NO:2 and SEQ ID NO:3) is as shown in Figure 1 to the partial results of 480 individuals amplification.
Whole electrophoresis result are carried out data statistic analysis; Concrete grammar is following: be a that is designated as of male parent type with F2 colony individual plant amplified band; Amplified band is the b that is designated as of maternal type; Amplified band contains the h that is designated as of male parent type and maternal type simultaneously, all do not have be designated as-, finally obtain the genotype data of 616 pairs of primer amplifications of F2 colony 480 individuals.Such as, the data of 480 individuals that obtain with first pair of primer are a, b, and h ,-; B ... totally 480 data, the data of using second pair of primer to obtain are b, a, h; A ,-... totally 480 data, totally 616 pairs of primers are added up respectively, and gained is the genotype data of this F2 colony.
With MapMaker 3.0 softwares (Constructing genetic maps withMAPMAKER/EXP 3.0, S Lincoln, M Daly; E Lander-Cambridge; MA:Whitehead Institute, 1992) carry out the genetic linkage maps drafting, obtain genetic linkage map.The position of the clear 616 pairs of primers of genetic linkage map subscript reaches the genetic distance with anti-herbicide gene.
According to the antiweed phenotype of 480 individuals, similar with the male parent type proterties a (anti-Sethoxydin) that is designated as, similar with the maternal type proterties b (not anti-Sethoxydin) that is designated as, proterties occupy and is designated as h (the anti-Sethoxydin of part) between male parent and the female parent.Obtain the phenotypic data of 480 individuals, the phenotypic data of 480 individuals compares with the genotype data of 480 individuals that obtain before, and similar Gao Ze represents this mark and antiweed (the anti-Sethoxydin of anti-Sethoxydin or part) proterties close linkage.
According to The above results with antiweed (Sethoxydin) assignment of genes gene mapping on genetic linkage maps.
Embodiment 6: with the checking of the closely linked molecule marker of millet antiweed
1. on the basis of the genetic linkage maps that embodiment 5 makes; According to the genetic linkage distance of millet anti-herbicide gene; With the hereditary close linkage distance of millet anti-herbicide gene for having confirmed molecule marker primer (SEQ ID NO:2 and SEQ ID NO:3) in the position of 0.5cM; And find corresponding male parent sequence location, and the sequence between the upstream and downstream primer is molecule marker, and its nucleotide sequence is shown in SEQ ID NO:1.
2. in addition; In the electrophoresis in embodiment 5 to the pcr amplification product of 480 individuals in F2 generation; Amplification for molecule marker primer (SEQ ID NO:2 and SEQ ID NO:3) is: the amplified production of 360 anti-Sethoxydin types and the anti-Sethoxydin type of part all has the band of 553bp size, and the pcr amplification product of 120 not anti-Sethoxydin types does not all have the band (the part amplification is as shown in Figure 1) of 553bp.And the fragments sequence through this 553bp of order-checking proof is identical with SEQ ID NO:1.
It is thus clear that molecule marker of the present invention (SEQ ID NO:1) is and the closely linked molecule marker of millet antiweed (Sethoxydin) gene.
Although embodiment of the present invention has obtained detailed description, it will be understood to those of skill in the art that.According to disclosed all instructions, can carry out various modifications and replacement to those details, these change all within protection scope of the present invention.Four corner of the present invention is provided by accompanying claims and any equivalent thereof.
Claims (11)
- One kind with the closely linked molecule marker of millet anti-herbicide gene, its nucleotide sequence is shown in SEQ ID NO:1, wherein, said weedicide is a Sethoxydin.
- 2. recombinant vectors, it contains the described molecule marker of claim 1.
- 3. reconstitution cell, it contains the described recombinant vectors of claim 2.
- 4. the primer of the described molecule marker of claim 1, its nucleotide sequence is shown in SEQ ID NO:2 and SEQ ID NO:3.
- 5. the preparation method of the described molecule marker of claim 1 comprises the steps:The genomic dna of millet that uses anti-Sethoxydin type or the anti-Sethoxydin type of part carries out pcr amplification as template with the described primer of claim 4;Wherein, the millet of said anti-Sethoxydin type is for opening paddy No. 1 or opening the anti-Sethoxydin type of F2 in generation that No. 3 selfings of confused flour beetle produce; The millet of the anti-Sethoxydin type of said part is for opening No. 3, confused flour beetle or opening the part anti-Sethoxydin type of F2 in generation that No. 3 selfings of confused flour beetle produce.
- 6. preparation method according to claim 5, it also comprises the step of the product of pcr amplification being carried out purifying.
- 7. the detection method of the described molecule marker of claim 1 comprises the steps:(1) according to the nucleotide sequence design primer of the molecule marker of claim 1;(2) genomic dna with millet to be detected increases as template;(3) judge whether there is this molecule marker in the amplified production;Wherein, said primer is the described primer of claim 4.
- 8. the described molecule marker of claim 1 is in millet anti-herbicide gene location or the purposes in detecting; Wherein, said weedicide is a Sethoxydin.
- 9. the localized method of millet anti-herbicide gene comprises the step of using the described molecule marker of claim 1; Wherein, said weedicide is a Sethoxydin.
- 10. the purposes of the described molecule marker of claim 1 in the millet assistant breeding, wherein, detect have a described molecule marker of claim 1 be anti-Sethoxydin type or the anti-Sethoxydin type of part, do not have the not anti-Sethoxydin type that is of this molecule marker.
- 11. millet auxiliary breeding means; Comprise that test right requires the step of 1 described molecule marker; Wherein, detect have the described molecule marker of claim 1 for anti-Sethoxydin type or the anti-Sethoxydin type of part, do not have the not anti-Sethoxydin type that is of this molecule marker.
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| CN102690818B (en) * | 2011-03-24 | 2016-06-01 | 深圳华大基因科技有限公司 | Molecular marker SIsv0832 closely linked with millet Heading date gene |
| CN102690812B (en) * | 2011-03-24 | 2016-06-01 | 深圳华大基因科技有限公司 | Molecular marker SIsv0067 closely linked with millet Heading date gene |
| CN102690811B (en) * | 2011-03-24 | 2016-06-01 | 深圳华大基因科技有限公司 | Molecular marker SIsv1363 closely linked with millet leaf color gene |
| CN102925435B (en) * | 2012-10-10 | 2014-08-20 | 上海交通大学 | Molecular markers closely linked to tuberculate fruit gene Tu in cucumber |
| CN106916813A (en) * | 2015-12-28 | 2017-07-04 | 深圳华大农业与循环经济科技有限公司 | With the molecular labeling SVhd3 of millet breeding time gene close linkage |
| CN108660231A (en) * | 2017-12-08 | 2018-10-16 | 深圳华大小米产业股份有限公司 | With the molecular labeling SIsv1208 of the anti-Sethoxydin herbicide resistance gene close linkage of millet |
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