CN101972348A - Method for extracting total saponin from leaves of rehmannia - Google Patents

Abstract

The invention discloses a method for extracting the total saponin from the leaves of rehmannia. In the method, a countercurrent extraction manner is adopted to extract the total saponin from the leaves of the rehmannia. The extraction method has the characteristics of high extraction efficiency, energy conservation, small production and operation difficulty, low labor intensity, low field pollution, less material waste, high content of active constituent, good quality and the like.

Description

A kind of extracting method of Folium Rehmanniae total glycosides

Technical field

The invention belongs to technical field of Chinese medicines, particularly relate to a kind of extracting method of Folium Rehmanniae total glycosides.

Background technology

Folium Rehmanniae is the dried leaves of scrophulariaceae rehmannia glutinosa plant (Rehmannia glutinosa Libosch).The Folium Rehmanniae main product also has wild on China Shaanxi, Hebei and other places and cultivation in celebrating area, bosom, Henan Province.Modern pharmacology studies show that contained main component is the Folium Rehmanniae total glycosides in the Folium Rehmanniae, and the Folium Rehmanniae total glycosides has enriching yin and nourishing kidney, removing heat from blood and promoting blood circulation, the effect of consolidating semen hemostasis.Can be used for treating albuminuria, hematuria, chronic glomerulonephritis; Belong to syndrome of deficiency of both qi and yin.The extraction of Folium Rehmanniae total glycoside extract is one of important step in the total glycosides formulation production process of Folium Rehmanniae, and product quality is had very big influence.The extracting method of Folium Rehmanniae total glycoside extract mainly adopts the multi-function extractor reflux, extract, at present.At Chinese patent disclosed " Folium Rehmanniae extract and its production and use " (publication number: CN1947757A) with " preparation technology who has the verbascoside of treatment chronic glomerulonephritis effect in the Folium Rehmanniae " (publication number: CN101121740A), all adopt the method.The problem that this method exists is: adopt industry reflux, extract, commonly used, it is big to add the solvent amount, causes when concentrating heat time heating time long, and power consumption is high, and the active substance decomposition is many, and finished product content is few, and promptly Folium Rehmanniae total glycosides dry products is of poor quality; The every batch of extraction, concentration time are long, cause the production cycle long, the production cost height.

Summary of the invention

Purpose of the present invention provides the new extracting method of a kind of Folium Rehmanniae total glycosides.To overcome the problem that above-mentioned use multi-function extractor reflux, extract, exists, the extracting method production efficiency height that the present invention uses, energy utilization rate height, the product quality height, and produce controlled, production environment good, GMP compatible can reduce production costs simultaneously greatly.

The present invention includes following steps:

(1) extract:

Get Folium Rehmanniae and drop in the extraction pot, add 80% ethanol that contains 0.5% acetic acid of 8 times of medical material amounts first the first time, reflux 1h, sucking filtration is collected filtrate; 80% ethanol that contains 0.5% acetic acid that adds for the second time 8 times of medical material amounts, reflux 1h, sucking filtration, collect filtrate, extract filtrate as second batch of primary extraction solvent of Folium Rehmanniae for the second time, adopt second batch the second time new solvent to extract, filter the back as the 3rd batch of primary extraction solvent, the rest may be inferred, and every batch extracting solution is concentrated for the first time, and last batch of concentrates extracted twice liquid;

(2) concentrated and cooling is left standstill:

Get every batch of the 1st extracting solution and add in the concentrator, relative density is 1.02～1.04g/ml when being concentrated into temperature and reaching 50～60 ℃, concentrated solution, the concentrated solution cooling is left standstill to room temperature, promptly.

Wherein: described the concentrating of step (2) is in haplo-effect concentrator, adopts 60～70 ℃ of concentrating under reduced pressure.

Beneficial effect of the present invention

The present invention adopts the countercurrent extraction mode to extract, promptly before batch last extracting solution as second batch of extraction solvent for the first time, every batch of extracting solution concentration for the first time.Through repeatedly producing checking, the dissolubility of Folium Rehmanniae active component in solvent is bigger, and 70～80% material stripping is arranged when extracting for the first time, and remaining 20～30% material can be extracted out for the second time.That is to say: when extracting second batch, the solvent that adds has contained has criticized 20～30% active substance inside, after refluxing, dissolved in 70～80% the active substance of this batch in these solvents again, that current extracting solution has contained 100% active substance in fact, promptly be that medicinal material extract is complete, the extracting solution of these 8 times of amounts concentrated promptly get a collection of extraction product again.Can find out that thus concentrated liquid measure has reduced half, the corresponding time also shortens 1/2, and the material heated time is few, and the active substance of reservation improves more than 20% than former method, so the content height of dried cream, quality is good.Concentration time is short, and energy conservation reaches more than 40%.Folium Rehmanniae total glycoside extract employing countercurrent extraction has the extraction efficiency height, energy conservation is many, and the production operation difficulty is little, labor intensity is low, on-the-spot pollution is low, material less wastage, active constituent content height, superior quality characteristics.

The specific embodiment

(1) extract:

Get Folium Rehmanniae and drop in the extraction pot, add 80% ethanol that contains 0.5% acetic acid of 8 times of medical material amounts first the first time, reflux 1h, sucking filtration is collected filtrate; 80% ethanol that contains 0.5% acetic acid that adds for the second time 8 times of medical material amounts, reflux 1h, sucking filtration, collect filtrate, extract filtrate as second batch of primary extraction solvent of Folium Rehmanniae for the second time, adopt second batch the second time new solvent to extract, filter the back as the 3rd batch of primary extraction solvent, the rest may be inferred, and every batch extracting solution is concentrated for the first time, and last batch of concentrates extracted twice liquid;

(2) concentrated and cooling is left standstill:

Get every batch of the 1st extracting solution and add in the concentrator, relative density is 1.02～1.04g/ml when being concentrated into temperature and reaching 50～60 ℃, concentrated solution, the concentrated solution cooling is left standstill to room temperature, promptly.

Claims (2)

1. the extracting method of a Folium Rehmanniae total glycosides is characterized in that comprising the steps:

(1) extract:

Get Folium Rehmanniae and drop in the extraction pot, add 80% ethanol that contains 0.5% acetic acid of 8 times of medical material amounts first the first time, reflux 1h, sucking filtration is collected filtrate; 80% ethanol that contains 0.5% acetic acid that adds for the second time 8 times of medical material amounts, reflux 1h, sucking filtration, collect filtrate, extract filtrate as second batch of primary extraction solvent of Folium Rehmanniae for the second time, adopt second batch the second time new solvent to extract, filter the back as the 3rd batch of primary extraction solvent, the rest may be inferred, and every batch extracting solution is concentrated for the first time, and last batch of concentrates extracted twice liquid;

(2) concentrated and cooling is left standstill:

Get every batch of the 1st extracting solution and add in the concentrator, relative density is 1.02～1.04g/ml when being concentrated into temperature and reaching 50～60 ℃, concentrated solution, the concentrated solution cooling is left standstill to room temperature, promptly.

2. the extracting method of a kind of Folium Rehmanniae total glycosides according to claim 1 is characterized in that: in the described step (2), concentrating is in haplo-effect concentrator, adopts 60～70 ℃ of concentrating under reduced pressure.