CN101968492B - Particle-enhanced turbidimetric immune assay kit for detecting adiponectin and preparation method thereof - Google Patents
Particle-enhanced turbidimetric immune assay kit for detecting adiponectin and preparation method thereof Download PDFInfo
- Publication number
- CN101968492B CN101968492B CN 201010526950 CN201010526950A CN101968492B CN 101968492 B CN101968492 B CN 101968492B CN 201010526950 CN201010526950 CN 201010526950 CN 201010526950 A CN201010526950 A CN 201010526950A CN 101968492 B CN101968492 B CN 101968492B
- Authority
- CN
- China
- Prior art keywords
- adiponectin
- preparation
- mass percent
- latex
- particle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108010076365 Adiponectin Proteins 0.000 title claims abstract description 97
- 102000011690 Adiponectin Human genes 0.000 title claims abstract description 97
- 238000002360 preparation method Methods 0.000 title claims abstract description 49
- 239000002245 particle Substances 0.000 title claims abstract description 36
- 238000003149 assay kit Methods 0.000 title abstract 3
- 239000004816 latex Substances 0.000 claims abstract description 70
- 229920000126 latex Polymers 0.000 claims abstract description 70
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 239000000725 suspension Substances 0.000 claims abstract description 49
- 239000006172 buffering agent Substances 0.000 claims abstract description 29
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 24
- 238000001514 detection method Methods 0.000 claims abstract description 24
- 239000004094 surface-active agent Substances 0.000 claims abstract description 22
- 239000011780 sodium chloride Substances 0.000 claims abstract description 12
- 239000003381 stabilizer Substances 0.000 claims abstract description 11
- 238000006243 chemical reaction Methods 0.000 claims description 26
- 238000003018 immunoassay Methods 0.000 claims description 25
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 14
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 11
- 101000775469 Homo sapiens Adiponectin Proteins 0.000 claims description 11
- 239000007853 buffer solution Substances 0.000 claims description 11
- 230000002421 anti-septic effect Effects 0.000 claims description 10
- 102000057799 human ADIPOQ Human genes 0.000 claims description 10
- 229920002521 macromolecule Polymers 0.000 claims description 10
- 210000002966 serum Anatomy 0.000 claims description 9
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 8
- 239000004471 Glycine Substances 0.000 claims description 7
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 6
- 244000309466 calf Species 0.000 claims description 6
- 235000013905 glycine and its sodium salt Nutrition 0.000 claims description 6
- 239000010452 phosphate Substances 0.000 claims description 6
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 6
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 238000012856 packing Methods 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 229920002594 Polyethylene Glycol 8000 Polymers 0.000 claims description 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 4
- 229920004890 Triton X-100 Polymers 0.000 claims description 4
- 239000004005 microsphere Substances 0.000 claims description 4
- 239000012467 final product Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000013207 serial dilution Methods 0.000 claims description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 2
- 101100260020 Caenorhabditis elegans mls-1 gene Proteins 0.000 claims description 2
- 229940098773 bovine serum albumin Drugs 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 1
- 229960003511 macrogol Drugs 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 238000003556 assay Methods 0.000 abstract description 5
- 230000000840 anti-viral effect Effects 0.000 abstract 6
- 239000011248 coating agent Substances 0.000 abstract 6
- 238000000576 coating method Methods 0.000 abstract 6
- 239000003755 preservative agent Substances 0.000 abstract 1
- 230000002335 preservative effect Effects 0.000 abstract 1
- 238000013016 damping Methods 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 5
- 208000029078 coronary artery disease Diseases 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 238000004879 turbidimetry Methods 0.000 description 4
- 201000001320 Atherosclerosis Diseases 0.000 description 3
- 239000008118 PEG 6000 Substances 0.000 description 3
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 108010074051 C-Reactive Protein Proteins 0.000 description 2
- 208000008589 Obesity Diseases 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000003759 clinical diagnosis Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 235000020824 obesity Nutrition 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 102100031786 Adiponectin Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 102100032752 C-reactive protein Human genes 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102000012192 Cystatin C Human genes 0.000 description 1
- 108010061642 Cystatin C Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010060378 Hyperinsulinaemia Diseases 0.000 description 1
- 206010022489 Insulin Resistance Diseases 0.000 description 1
- 108010033266 Lipoprotein(a) Proteins 0.000 description 1
- 102000057248 Lipoprotein(a) Human genes 0.000 description 1
- 102000002710 Neurophysins Human genes 0.000 description 1
- 108010018674 Neurophysins Proteins 0.000 description 1
- 206010033307 Overweight Diseases 0.000 description 1
- 208000005764 Peripheral Arterial Disease Diseases 0.000 description 1
- 208000030831 Peripheral arterial occlusive disease Diseases 0.000 description 1
- HVVWZTWDBSEWIH-UHFFFAOYSA-N [2-(hydroxymethyl)-3-prop-2-enoyloxy-2-(prop-2-enoyloxymethyl)propyl] prop-2-enoate Chemical compound C=CC(=O)OCC(CO)(COC(=O)C=C)COC(=O)C=C HVVWZTWDBSEWIH-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000007211 cardiovascular event Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 230000003451 hyperinsulinaemic effect Effects 0.000 description 1
- 201000008980 hyperinsulinism Diseases 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000028867 ischemia Diseases 0.000 description 1
- 230000000803 paradoxical effect Effects 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Images
Landscapes
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (12)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201010526950 CN101968492B (en) | 2010-10-29 | 2010-10-29 | Particle-enhanced turbidimetric immune assay kit for detecting adiponectin and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201010526950 CN101968492B (en) | 2010-10-29 | 2010-10-29 | Particle-enhanced turbidimetric immune assay kit for detecting adiponectin and preparation method thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101968492A CN101968492A (en) | 2011-02-09 |
CN101968492B true CN101968492B (en) | 2013-01-09 |
Family
ID=43547677
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 201010526950 Active CN101968492B (en) | 2010-10-29 | 2010-10-29 | Particle-enhanced turbidimetric immune assay kit for detecting adiponectin and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN101968492B (en) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102253217B (en) * | 2011-04-07 | 2013-08-07 | 武汉生之源生物科技有限公司 | Detection kit of latex particle enhanced neutrophil gelatinase-associated lipid transfer protein |
CN102331494B (en) * | 2011-06-16 | 2014-03-26 | 广州艺佳生物科技有限公司 | Sealing and stabilizing agent for microporous board |
CN102507926B (en) * | 2011-10-12 | 2014-02-05 | 安徽信灵检验医学科技有限公司 | Gold-coated polystyrene nanoparticles and preparation method |
CN102628868B (en) * | 2011-12-30 | 2014-09-17 | 北京九强生物技术股份有限公司 | Latex enhanced immunoturbidimetry kit for detection of asymmetric dimethylarginine content |
CN102636653A (en) * | 2012-04-19 | 2012-08-15 | 上海蓝怡科技有限公司 | Compounded latex particle-enveloped cystatin C detection kit |
CN102662064A (en) * | 2012-04-26 | 2012-09-12 | 苏州照康生物技术有限公司 | Immunonephelometry kit for detecting lipid carrier protein related to neutrophils gelatinase and preparation method thereof |
CN102662059B (en) * | 2012-05-11 | 2014-08-13 | 北京美康生物技术研究中心 | Latex-enhanced immunoturbidimetry kit for measuring Helicobacter pylori antibody as well as preparation method and application thereof |
CN102879569A (en) * | 2012-09-18 | 2013-01-16 | 苏州照康生物技术有限公司 | Immunoturbidimetry kit for detecting cysteine proteinase inhibitor C and preparation method thereof |
CN104237522B (en) * | 2012-12-03 | 2016-12-21 | 武汉生之源生物科技股份有限公司 | Adiponectin reagent box for detecting content and preparation method thereof |
WO2014189624A1 (en) * | 2013-05-20 | 2014-11-27 | Silicon Biodevices, Inc. | Digital control of on-chip magnetic particle assay |
CN103777023B (en) * | 2013-12-16 | 2015-04-08 | 深圳市安之酶生物技术有限公司 | Kit for measuring adiponectin by nano latex enhanced immune turbidimetry |
CN103675299A (en) * | 2013-12-24 | 2014-03-26 | 上海北加生化试剂有限公司 | Kit and method for detecting concentration of fibronectin in urine |
CN104407154B (en) * | 2014-12-05 | 2016-03-30 | 重庆中元生物技术有限公司 | A kind of high sensitivity latex enhancing immune turbidimetry kit |
CN105759061B (en) * | 2016-02-26 | 2018-06-19 | 厦门大学附属中山医院 | Human serum adiponectin detection kit based on microparticle chemiluminescence immunoassay |
CN106290822B (en) * | 2016-07-28 | 2018-06-19 | 武汉景川诊断技术股份有限公司 | Latex microsphere preparation method and application is immunized in d-dimer |
CN110007091A (en) * | 2019-04-02 | 2019-07-12 | 杭州博谱医药科技有限公司 | A kind of adiponectin detection kit and preparation method thereof |
CN110907639A (en) * | 2019-12-05 | 2020-03-24 | 四川新健康成生物股份有限公司 | Serum amyloid protein A detection kit and preparation method thereof |
CN111239421A (en) * | 2020-02-19 | 2020-06-05 | 安徽大千生物工程有限公司 | Latex-enhanced immunoturbidimetry kit for quantitatively detecting adiponectin ADPN and preparation and application methods thereof |
CN114112957B (en) * | 2021-11-26 | 2022-06-21 | 浙江夸克生物科技有限公司 | Adiponectin determination kit and application thereof |
CN114137229A (en) * | 2021-12-03 | 2022-03-04 | 苏州普瑞斯生物科技有限公司 | Adiponectin detection reagent production process adopting latex enhanced immunoturbidimetry |
CN114295840B (en) * | 2021-12-29 | 2023-04-07 | 中元汇吉生物技术股份有限公司 | Kit for high-sensitivity quantitative determination of adiponectin and preparation method thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN202066863U (en) * | 2010-10-29 | 2011-12-07 | 厦门大学附属中山医院 | Particle-enhanced turbidimetric immunoassay kit for adiponectin detection |
-
2010
- 2010-10-29 CN CN 201010526950 patent/CN101968492B/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN202066863U (en) * | 2010-10-29 | 2011-12-07 | 厦门大学附属中山医院 | Particle-enhanced turbidimetric immunoassay kit for adiponectin detection |
Non-Patent Citations (6)
Title |
---|
人血清脂联素定量ELISA检测方法的建立及初步临床应用;李登清、陈琳磊等;《第二军医大学学报》;20090731;第30卷(第7期);850-853 * |
人血清脂联素酶联免疫吸附试验测定方法的建立及临床应用研究;魏文青、赵满仓等;《标记免疫分析与临床》;20090630;第16卷(第3期);166-169 * |
抗脂联素单克隆抗体的制备及免疫学特性分析;赵满仓、魏文青等;《华北国防医药》;20081231;第20卷(第6期);35-37 * |
李登清、陈琳磊等.人血清脂联素定量ELISA检测方法的建立及初步临床应用.《第二军医大学学报》.2009,第30卷(第7期),850-853. |
赵满仓、魏文青等.抗脂联素单克隆抗体的制备及免疫学特性分析.《华北国防医药》.2008,第20卷(第6期),35-37. |
魏文青、赵满仓等.人血清脂联素酶联免疫吸附试验测定方法的建立及临床应用研究.《标记免疫分析与临床》.2009,第16卷(第3期),166-169. |
Also Published As
Publication number | Publication date |
---|---|
CN101968492A (en) | 2011-02-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101968492B (en) | Particle-enhanced turbidimetric immune assay kit for detecting adiponectin and preparation method thereof | |
CN202066863U (en) | Particle-enhanced turbidimetric immunoassay kit for adiponectin detection | |
Eda et al. | Development of a new microparticle‐enhanced turbidimetric assay for C‐reactive protein with superior features in analytical sensitivity and dynamic range | |
CN102749454B (en) | Full-scale C-reactive protein (CRP) colloidal gold immunoturbidimetric assay kit | |
CN106324239B (en) | Latex microsphere preparation method and application is immunized in C reactive protein | |
CN102253217B (en) | Detection kit of latex particle enhanced neutrophil gelatinase-associated lipid transfer protein | |
CN101377492B (en) | Bladder chalone C determining reagent kit | |
Poulsen et al. | A luminescent oxygen channeling immunoassay for the determination of insulin in human plasma | |
CN101452001B (en) | Quantitative determination RBP4 kit by chemiluminescence magnetic enzymoimmune method | |
CN108303544A (en) | A kind of whole blood c reactive protein detection kit | |
US20110318847A1 (en) | Cvd assay | |
Nishimura et al. | Determination of adiponectin in serum using a latex particle-enhanced turbidimetric immunoassay with an automated analyzer | |
CN110208549A (en) | A kind of Soluble growth stimulation 2 albumen sST2 luminescence reagent box of expressing gene | |
CN102692504A (en) | D-dimer quantitative fluorescence immunoassay test strip and preparation method thereof | |
Machida et al. | Determination of serum lipoprotein lipase using a latex particle-enhanced turbidimetric immunoassay with an automated analyzer | |
CN102539784B (en) | Method for detecting cardiac troponin I and application thereof | |
CN102662064A (en) | Immunonephelometry kit for detecting lipid carrier protein related to neutrophils gelatinase and preparation method thereof | |
Chen et al. | Chemiluminescence immunoassay for sensing lipoprotein-associated phospholipase A2 in cardiovascular risk evaluation | |
CN106053862B (en) | Serum amyloid A protein(SAA)Determine kit | |
CN108570104A (en) | Recombinating adiponectin antigen, antibody and adiponectin nano rubber latex enhances immunoturbidimetry kit | |
Lachno et al. | Validation of assays for measurement of amyloid-β peptides in cerebrospinal fluid and plasma specimens from patients with Alzheimer's disease treated with solanezumab | |
Bakker | Immunoturbidimetry of urinary albumin: prevention of adsorption of albumin; influence of other urinary constituents. | |
CN112763731A (en) | Lipoprotein (a) determination kit and detection method thereof | |
WO2010013525A1 (en) | Method of assaying complex and kit to be used therefor | |
CN108918888A (en) | It is a kind of detect Endostatin latex enhancing immune than turbid kit and its application |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20160816 Address after: Beijing road in Baohe District of Hefei city in Anhui province and the intersection of Dalian road 230051 Patentee after: ANHUI IPROCOM BIOTECHNOLOGY Co.,Ltd. Address before: Siming Lake Road Xiamen city Fujian province 361004 No. 201-209 Patentee before: Zhongshan Hospital Xiamen University |
|
CP02 | Change in the address of a patent holder | ||
CP02 | Change in the address of a patent holder |
Address after: 230051 Floor 1-4 of Building 18, Liandong U Valley Phase I, southeast corner of busy road and Jilin Road intersection, Baohe Economic Development Zone, Hefei City, Anhui Province Patentee after: ANHUI IPROCOM BIOTECHNOLOGY Co.,Ltd. Address before: Beijing road in Baohe District of Hefei city in Anhui province and the intersection of Dalian road 230051 Patentee before: ANHUI IPROCOM BIOTECHNOLOGY Co.,Ltd. |
|
CP02 | Change in the address of a patent holder | ||
CP02 | Change in the address of a patent holder |
Address after: No.659 Lanzhou Road, Baohe District, Hefei City, Anhui Province 230051 Patentee after: ANHUI IPROCOM BIOTECHNOLOGY Co.,Ltd. Address before: 230051 Floor 1-4 of Building 18, Liandong U Valley Phase I, southeast corner of busy road and Jilin Road intersection, Baohe Economic Development Zone, Hefei City, Anhui Province Patentee before: ANHUI IPROCOM BIOTECHNOLOGY Co.,Ltd. |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Particle enhanced immunoturbidimetric assay kit for detecting adiponectin and its preparation method Granted publication date: 20130109 Pledgee: Hefei Binhu fountainhead financing Company limited by guarantee Pledgor: ANHUI IPROCOM BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024980036334 |