CN101968492A - Particle-enhanced turbidimetric immune assay kit for detecting adiponectin and preparation method thereof - Google Patents
Particle-enhanced turbidimetric immune assay kit for detecting adiponectin and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a particle-enhanced turbidimetric immune assay kit for detecting adiponectin and a preparation method thereof, in particular to a detection method of a turbidimetric immune assay. The particle-enhanced turbidimetric immune assay kit for detecting the adiponectin comprises a kit body, an application liquid bottle and a latex suspension bottle coating an antiviral adiponectin monoclonal antibody, wherein the application liquid bottle is filled with application liquid; the application liquid comprises a surfactant, a preservative, a macromolecular accelerator, sodium chloride and a buffering agent; the latex suspension bottle coating the antiviral adiponectin monoclonal antibody is filled with a latex suspension coating the antiviral adiponectin monoclonal antibody; and the latex suspension coating the antiviral adiponectin monoclonal antibody comprises latex coating the antiviral adiponectin monoclonal antibody, the surfactant, a stabilizer and the buffering agent. The preparation method comprises the following steps of: preparing a latex antibody; preparing the latex suspension coating the antiviral adiponectin monoclonal antibody; preparing the application liquid; and finally preparing adiponectin series standard products.
Description
Technical field
The present invention relates to the turbid detection method of a kind of immune transmittance, especially relate to a kind of turbid kit of particle enhance immunity transmittance that detects adiponectin and preparation method thereof.
Background technology
Adiponectin (adiponectin) also claim Acrp30, GBP28 or AdipoQ, be to secrete a kind of neurophysin by adipocyte, can be in human plasma stable existence, concentration range is about 3.0～17.0mg/L, account for 0.01% ([1] Arita Y of plasma proteins, Kihara S, Ouchi N, et al.Paradoxical decrease of an adipose-specific protein, adiponectin, inobesity[J] .Biochem Biophys Res Commun, 1999,257 (1): 79-83).Along with deepening continuously to adiponectin research, find, the concentration of adiponectin in obesity patient and type ii diabetes patients serum is starkly lower than non-overweight people, the reduction of its concentration has caused insulin resistance and hyperinsulinemia, and adiponectin has the effect that prevents that atherosclerotic plaque from forming, therefore, the generation development of adiponectin and obesity patient's type ii diabetes and coronary heart disease is closely related, the obvious reduction that is adiponectin is indicating that the generation of type ii diabetes and coronary heart disease obviously increases ([2] Zhang MH, Spies C, Ali S, et al.Adiponectinand inducible ischemia in patients with stable coronary heart disease:data from the Heart and Soulstudy[J] .Atherosclerosis, 2009,205 (1): 233-238; [3] Kawano J, Arora R.The role of adiponectin inobesity, diabetes, and cardiovascular disease[J] .J Cardiometab Syndr.2009,4 (1): 44-49).Measure adiponectin level in the blood, significant to the diagnosis and the prognosis of these diseases.
At present, the clinical diagnosis technology of adiponectin mainly comprises: radioimmunology (RIA) ([4] Schulze MB, Shai I, RimmEB, et al.Adiponectin and future coronary heart disease events among men with type 2diabetes[J] .Diabetes, 2005,54 (2): 534-539), enzyme linked immune assay (ELISA) ([5] Urbonaviciene G, Frystyk J, Flyvbjerg A, et al.Association of serum adiponectin with risk for cardiovascular events in patientswith peripheral arterial disease[J] .Atherosclerosis, 2010,210 (2): method such as 619-624), their advantage is that sensitivity is higher, shortcoming is a complex operation, length consuming time is subject to manual operation and extraneous factor and disturbs, and automaticity is low, and radioactively labelled substance can produce harm to the operator, and environment is polluted.These drawbacks limit applying of existing adiponectin detection method, make it can't be widely used in clinical diagnosis and research work.
Particle enhance immunity turbidimetry (particle-enhanced turbidimetric immunoassay, PETIA) be occur in recent years a kind of comparatively stable, body fluid albumen homogeneous phase immunoturbidimetry detection method accurately, its ultimate principle is the surface-crosslinked monoclonal or the polyclonal antibody of certain grain size latex particle, when the crosslinked microballoon that antibody arranged with after antigen combines, can flock together rapidly at short notice, change the absorbance of reactant liquor.And the concentration of this change and tested antigen has stronger correlativity, can reflect the concentration of tested antigen within the specific limits.The mode that this technology strengthens by particle, amplified antigen-antibody reaction, remedied the not high deficiency of common turbidimetry sensitivity, turbidimetry good stability, advantage have easily and fast been inherited simultaneously again, overcome the shortcoming of ELISA and RIA method, more and more widely be applied to the detection by quantitative of various traces of albumin clinically.At present, the latex enhancing immune turbidimetry has been widely used in bladder chalone C ([6] Al-Turkmani MR, Law T, Kellogg MD.Performance evaluation of a particle-enhanced turbidimetric cystatin C assay on theHitachi 917analyzer[J] .Clin Chim Acta.2008,398 (1-2): 75-77), lipoprotein (a), c reactive protein, super sensitive C-reactive protein, the detection of antistreptolysin O (ASO), obtain good social benefit, but do not see have this type of adiponectin detection kit to come out both at home and abroad so far as yet.
Summary of the invention
The object of the present invention is to provide a kind of turbid kit of particle enhance immunity transmittance that detects adiponectin and preparation method thereof.
The turbid kit of particle enhance immunity transmittance of detection adiponectin of the present invention, comprise that box body, application liquid bottle and bag are by the latex suspension bottle of anti-human adiponectin monoclonal antibody, described application liquid bottle and bag are positioned in the box body by the latex suspension bottle of anti-human adiponectin monoclonal antibody, dress is used liquid in the described application liquid bottle, and the composition of described application liquid and content by mass percentage thereof are surfactant 0.1%～0.5%, antiseptic 0.05%～1%, macromolecule accelerator 2%～8%, sodium chloride 0.1%～10% and buffering agent 50～200mmol/L; By the latex suspension of anti-human adiponectin monoclonal antibody, described bag is that bag is by anti-human adiponectin monoclonal antibody latex 0.5%～5%, surfactant 0.1%～0.5%, stabilizing agent 0.1%～0.5% and buffering agent 10～100mmol/L by the composition of the latex suspension of anti-human adiponectin monoclonal antibody and content by mass percentage thereof to described bag by dress bag in the latex suspension bottle of anti-human adiponectin monoclonal antibody.
Surfactant in the described application liquid can be selected from Tween 20 or Triton-X100 etc.
Antiseptic in the described application liquid can be selected from Proclin 300 or Sodium azide etc.
Macromolecule accelerator in the described application liquid is optional from polyglycol 6000 (PEG6000) or polyglycol 8000 (PEG8000) etc.
Buffering agent in the described application liquid can be selected from trishydroxymethylaminomethane (Tris), phosphate or glycocoll etc.
Described bag can be selected from Tween 20 or Triton-X100 etc. by the surfactant in the latex suspension of anti-human adiponectin monoclonal antibody.
Described bag can be selected from bovine serum albumin(BSA) (BSA) etc. by the stabilizing agent in the latex suspension of anti-human adiponectin monoclonal antibody.
Described bag is selected from trishydroxymethylaminomethane (Tris), phosphate or glycocoll etc. by the buffering agent in the latex suspension of anti-human adiponectin monoclonal antibody.
Described application liquid buffering agent and latex SB preferably are consistent.
The preparation method of the turbid kit of particle enhance immunity transmittance of described detection adiponectin may further comprise the steps:
1) preparation latex antibody
Two kinds of mouse anti human adiponectin monoclonal antibodies are micro-sphere crosslinked with aldehyde radical respectively, get two kinds of latex antibody suspensions;
In step 1), described that two kinds of mouse anti human adiponectin monoclonal antibodies are micro-sphere crosslinked with aldehyde radical respectively, can adopt following concrete grammar: in carbonate buffer solution with the aldehyde radical microballoon respectively with two kinds of mouse anti human adiponectin monoclonal antibody mixings, behind 37 ℃ of revolving reactions, add the NaBH of carbonate buffer solution preparation
4Solution behind 4 ℃ of revolving reactions, adds the glycine solution of carbonate buffer solution preparation again, behind 4 ℃ of revolving reactions, cleans with the cleaning stock solution again, and gained latex antibody precipitation is resuspended in cleans in the stock solution, latex antibody suspension, place 4 ℃ of environment placements;
Described two kinds of mouse anti human adiponectin monoclonal antibodies can adopt clone:Adn37 antibody and clone:Adn26 antibody;
The aldehyde radical microballoon that described aldehyde radical microballoon can adopt Shanghai to provide according to the health bio tech ltd, the particle diameter of described aldehyde radical microballoon can be 100～300nm, is preferably 150nm, and the mass percent of aldehyde radical microballoon is 0.5%～5%;
Described carbonate buffer solution pH can be 8.5～9.6, is preferably 9.6; Mass percent is 20～100mmol/L, is preferably 50mmol/L.
The time of described 37 ℃ of revolving reactions can be 4～16h, is preferably 16h;
Described NaBH
4The mass percent of solution is 0.05～0.5g/L, is preferably 0.1g/L;
The time of described 4 ℃ of revolving reactions can be 2～4h, is preferably 4h;
The mass percent of described glycine solution can be 10～100mmol/L, is preferably 50mmol/L;
The described time of 4 ℃ of revolving reactions again can be 2～24h, is preferably 16h;
Described cleaning stock solution pH can be 7.4～8.8, is preferably 8.0, comprises buffering agent, surfactant and stabilizing agent.Buffering agent can be a phosphate, also can be trishydroxymethylaminomethane (Tris) or glycocoll, buffering agent shared mass percent in cleaning stock solution is 10～100mmol/L, be preferably 25mmol/L, the mass percent of surfactant and stabilizing agent is respectively 0.1%～0.5% and 0.05%～0.5%, is preferably 0.2% and 0.1%.
2) the preparation bag is by the latex suspension of anti-human adiponectin monoclonal antibody
Two kinds of latex antibody suspensions with the step 1) preparation mix respectively, must wrap by the latex suspension of anti-human adiponectin monoclonal antibody;
In step 2) in, described two kinds of latex antibody suspensions mix preferably two kinds of latex antibody suspension equal-volumes mixing.
3) liquid is used in preparation
Surfactant, antiseptic, macromolecule accelerator, sodium chloride and buffering agent are dissolved in the water in the lump fully, must use liquid;
In step 3), described buffering agent kind and step 2) in clean that buffering agent is consistent in the stock solution; Described application liquid pH value can be 7.4～8.0, is preferably 8.0; The shared mass percent of described surfactant can be 0.1%～0.5%, be preferably 0.2%, the shared mass percent of antiseptic can be 0.05%～1%, is preferably 0.1%, the shared mass percent of macromolecule accelerator can be 2%～8%, is preferably 4%; The shared mass percent of sodium chloride can be 0.1%～10%, is preferably 1.2%, and the mass percent of buffering agent can be 50～200mmol/L, is preferably 100mmol/L.
4) preparation adiponectin series standard product
Select the adiponectin standard items for use, use calf serum to carry out serial dilution: S6:40 μ g/ml, S5:20 μ g/ml, S4:10 μ g/ml, S3:5 μ g/ml, S2:1 μ g/mlS1:0.2 μ g/mlS0: calf serum, packing gets final product.
In step 4), described adiponectin standard items can be selected commercialization high-purity adiponectin standard items for use; Described packing can be adopted the packing of 0.5ml/ bottle.
Below provide the turbid kit of particle enhance immunity transmittance that adopts described detection adiponectin and on Biochemical Analyzer, carry out the actual conditions that adiponectin detects:
Setup parameter on Biochemical Analyzer:
Temperature of reaction: 37 ℃;
Analytical approach: 2 end-point methods;
Predominant wavelength: 570nm, commplementary wave length: 800nm (can);
Sample size/application liquid (R1)/bag is by the latex suspension (R2) of anti-human adiponectin monoclonal antibody: 5 μ l/200 μ l/50 μ l;
The Direction of Reaction: rise.
The present invention adopts anti-human adiponectin monoclonal antibody bag by the aldehyde radical microballoon, mode by the particle enhancing, amplified antigen-antibody reaction, remedied the not high deficiency of common turbidimetry sensitivity, turbidimetry good stability, advantage have easily and fast been inherited simultaneously again, overcome ELISA and RIA method complicated operation, bad etc. the shortcoming of repeatability, realize human blood adiponectin quantitatively fast detecting human adiponectin level in enormous quantities on biochemical instruments, conclude the outstanding advantage of getting up to have the following aspects: (1) is simple to operate; (2) sensitivity is higher, can reach 0.2 μ g/ml; (3) be not subject to manual operation and extraneous factor and disturb, detect stability and good reproducibility, can reflect the content of measured matter more truly; (4) can utilize Biochemical Analyzer to detect, realize easily robotization being suitable for clinical application.
Description of drawings
Fig. 1 is that the structure of the turbid kit embodiment of particle enhance immunity transmittance of detection adiponectin of the present invention is formed synoptic diagram.
Embodiment
Referring to Fig. 1, the turbid kit embodiment of particle enhance immunity transmittance of detection adiponectin of the present invention comprises box body 1, use liquid bottle 2 and bag by the latex suspension bottle 3 of anti-human adiponectin monoclonal antibody, described application liquid bottle 2 and bag are positioned in the box body 1 by the latex suspension bottle 3 of anti-human adiponectin monoclonal antibody, dress is used liquid in the described application liquid bottle, and the composition of described application liquid and content by mass percentage thereof are surfactant 0.1%～0.5%, antiseptic 0.05%～1%, macromolecule accelerator 2%～8%, sodium chloride 0.1%～10% and buffering agent 50～200mmol/L; By the latex suspension of anti-human adiponectin monoclonal antibody, described bag is that bag is by anti-human adiponectin monoclonal antibody latex 0.5%～5%, surfactant 0.1%～0.5%, stabilizing agent 0.1%～0.5% and buffering agent 10～100mmol/L by the composition of the latex suspension of anti-human adiponectin monoclonal antibody and content by mass percentage thereof to described bag by dress bag in the latex suspension bottle of anti-human adiponectin monoclonal antibody.
Preparation detects the turbid kit of particle enhance immunity transmittance of adiponectin
With 4mg aldehyde radical microballoon is example, and the key step of present embodiment comprises:
1. prepare latex antibody
Select for use high-purity, high-affinity two kinds of mouse anti human adiponectin monoclonal antibodies (clone:Adn37, clone:Adn26).Use 20mmol/L carbonate buffer solution (pH9.6) with the aldehyde radical microballoon respectively with two kinds of antibody with 10: 1 quality than mixing, 37 ℃, revolving reaction 16h, this moment, total reaction volume was 400 μ l; After reaction finishes, add the 4mg/mlNaBH of above-mentioned carbonate buffer solution preparation
4Solution 20 μ l, 4 ℃, reaction 4h; Add closed reagent 1mol/L glycine solution 20 μ l again, 4 ℃, reaction 16h; (contain Tween 200.2%, BSA 0.1%, pH7.4) cleans 3 times for 25mmol/L TBS damping fluid; Precipitation is resuspended in 4ml25mmol/L TBS damping fluid, and (contain Tween 20 0.2%, BSA 0.1%, pH7.4), latex suspension concentration is 1mg/ml.
2. two kinds of latex antibody mix, and the preparation bag is by the latex suspension of anti-human adiponectin monoclonal antibody
Two kinds of latex antibody suspension equal-volumes that will prepare respectively mix, and this is a R2 latex suspension.
3. liquid is used in preparation
The 50mmol/Ltris damping fluid of pH8.0 includes 12% NaCl, 4% PEG6000, and 0.1%Proclin 300.
4. the preparation of adiponectin series standard product
Select commercialization high-purity adiponectin standard items for use, use calf serum to carry out serial dilution: S6:40 μ g/ml, S5:20 μ g/ml, S4:10 μ g/ml, S3:5 μ g/ml, S2:1 μ g/ml, S1:0.2 μ g/mlS0: calf serum, the packing of 0.5ml/ bottle gets final product.
Preparation detects the turbid kit of particle enhance immunity transmittance of adiponectin
With 4mg aldehyde radical microballoon is example, and the key step of present embodiment comprises:
1. prepare latex antibody
With the aldehyde radical microballoon respectively with two kinds of antibody with 20: 1 quality than mixing, surplus with embodiment 1.
2. two kinds of latex antibody mix, and the preparation bag is by the latex suspension of anti-human adiponectin monoclonal antibody
With embodiment 1.
3. liquid is used in preparation
The 50mmol/Ltris damping fluid of pH7.4 includes 12% NaCl, 6% PEG6000, and 0.1%Proclin 300.
4. the preparation of adiponectin series standard product
With embodiment 1.
Preparation detects the turbid kit of particle enhance immunity transmittance of adiponectin
1. prepare latex antibody
To add 4mg/ml NaBH in the step 1 preparation latex antibody
4The amount of solution changes 10 μ l into, and 4 ℃, the reaction time changes 2h into, and is surplus with embodiment 1.
2. two kinds of latex antibody mix, and the preparation bag is by the latex suspension of anti-human adiponectin monoclonal antibody
With embodiment 1.
3. liquid is used in preparation
The 200mmol/Ltris damping fluid of pH8.0 includes 5.0% NaCl, 6% PEG8000, and 0.1%Proclin 300.
4. the preparation of adiponectin series standard product
With embodiment 1.
Embodiment 4
Preparation detects the turbid kit of particle enhance immunity transmittance of adiponectin
1. prepare latex antibody
2. two kinds of latex antibody mix, and the preparation bag is by the latex suspension of anti-human adiponectin monoclonal antibody
With embodiment 1.
3. liquid is used in preparation
With embodiment 1.
4. the preparation of adiponectin series standard product
With embodiment 1.
Embodiment 5
Carry out the detection of adiponectin on the Biochemical Analyzer
Setup parameter on 7060 type Biochemical Analyzers: 37 ℃ of temperature of reaction; Analytical approach: 2 end-point methods; Predominant wavelength: 570nm, commplementary wave length: 800nm; Sample size/R1/R2:5 μ l/250 μ l/50 μ l; The Direction of Reaction: rise.Read a little respectively at 20 and 34 points.After selecting the multiple spot calibration, instrument is finished calibration automatically, and behind the calibration OK, the adiponectin that carries out conventional serum specimen detects, and it directly is unit report numerical value with mg/L that the result converts automatically through instrument.
Claims (10)
1. detect the turbid kit of particle enhance immunity transmittance of adiponectin, it is characterized in that comprising that box body, application liquid bottle and bag are by the latex suspension bottle of anti-human adiponectin monoclonal antibody, described application liquid bottle and bag are positioned in the box body by the latex suspension bottle of anti-human adiponectin monoclonal antibody, dress is used liquid in the described application liquid bottle, and the composition of described application liquid and content by mass percentage thereof are surfactant 0.1%～0.5%, antiseptic 0.05%～1%, macromolecule accelerator 2%～8%, sodium chloride 0.1%～10% and buffering agent 50～200mmol/L; By the latex suspension of anti-human adiponectin monoclonal antibody, described bag is that bag is by anti-human adiponectin monoclonal antibody latex 0.5%～5%, surfactant 0.1%～0.5%, stabilizing agent 0.1%～0.5% and buffering agent 10～100mmol/L by the composition of the latex suspension of anti-human adiponectin monoclonal antibody and content by mass percentage thereof to described bag by dress bag in the latex suspension bottle of anti-human adiponectin monoclonal antibody.
2. the turbid kit of particle enhance immunity transmittance of detection adiponectin as claimed in claim 1 is characterized in that the surfactant in the described application liquid is selected from Tween 20 or Triton-X100; Antiseptic in the described application liquid is selected from Proclin 300 or Sodium azide; Macromolecule accelerator in the described application liquid is selected from Macrogol 6000 or polyglycol 8000; Buffering agent in the described application liquid is selected from trishydroxymethylaminomethane, phosphate or glycocoll.
3. the turbid kit of particle enhance immunity transmittance of detection adiponectin as claimed in claim 1 is characterized in that described bag is selected from Tween 20 or Triton-X100 by the surfactant in the latex suspension of anti-human adiponectin monoclonal antibody; Described bag is selected from bovine serum albumin(BSA) by the stabilizing agent in the latex suspension of anti-human adiponectin monoclonal antibody; Described bag is selected from trishydroxymethylaminomethane, phosphate or glycocoll by the buffering agent in the latex suspension of anti-human adiponectin monoclonal antibody.
4. the preparation method of the turbid kit of particle enhance immunity transmittance of detection adiponectin as claimed in claim 1 is characterized in that may further comprise the steps:
1) preparation latex antibody suspension
Two kinds of mouse anti human adiponectin monoclonal antibodies are micro-sphere crosslinked with aldehyde radical respectively, get two kinds of latex antibody suspensions;
2) the preparation bag is by the latex suspension of anti-human adiponectin monoclonal antibody
Two kinds of latex antibody suspensions with the step 1) preparation mix respectively, must wrap by the latex suspension of anti-human adiponectin monoclonal antibody;
3) liquid is used in preparation
Surfactant, antiseptic, macromolecule accelerator, sodium chloride and buffering agent are dissolved in the water in the lump fully, must use liquid;
4) preparation adiponectin series standard product
Select the adiponectin standard items for use, use calf serum to carry out serial dilution: S6:40 μ g/ml, S5:20 μ g/ml, S4:10 μ g/ml, S3:5 μ g/ml, S2:1 μ g/mlS1:0.2 μ g/mlS0: calf serum, packing gets final product.
5. the preparation method of the turbid kit of particle enhance immunity transmittance of detection adiponectin as claimed in claim 4, it is characterized in that in step 1), described that two kinds of mouse anti human adiponectin monoclonal antibodies are micro-sphere crosslinked with aldehyde radical respectively, adopt following concrete grammar: in carbonate buffer solution with the aldehyde radical microballoon respectively with two kinds of mouse anti human adiponectin monoclonal antibody mixings, behind 37 ℃ of revolving reactions, add the NaBH of carbonate buffer solution preparation
4Solution behind 4 ℃ of revolving reactions, adds the glycine solution of carbonate buffer solution preparation again, behind 4 ℃ of revolving reactions, cleans with the cleaning stock solution again, and gained latex antibody precipitation is resuspended in cleans in the stock solution, latex antibody suspension, place 4 ℃ of environment placements.
6. want the preparation method of the turbid kit of particle enhance immunity transmittance of 4 or 5 described detection adiponectins as right, it is characterized in that in step 1) described two kinds of mouse anti human adiponectin monoclonal antibodies adopt clone:Adn37 antibody and clone:Adn26 antibody; The particle diameter of described aldehyde radical microballoon is 100～300nm, is preferably 150nm, and the mass percent of aldehyde radical microballoon is 0.5%～5%.
7. the preparation method of the turbid kit of particle enhance immunity transmittance of detection adiponectin as claimed in claim 4 is characterized in that in step 1) described carbonate buffer solution pH is 8.5～9.6, is preferably 9.6; Mass percent is 20～100mmol/L, is preferably 50mmol/L;
The time of described 37 ℃ of revolving reactions is 4～16h, is preferably 16h;
Described NaBH
4The mass percent of solution is 0.05～0.5g/L, is preferably 0.1g/L;
The time of described 4 ℃ of revolving reactions is 2～4h, is preferably 4h;
The mass percent of described glycine solution is 10～100mmol/L, is preferably 50mmol/L;
The time of described 4 ℃ of revolving reactions again is 2～24h, is preferably 16h.
8. the preparation method of the turbid kit of particle enhance immunity transmittance of detection adiponectin as claimed in claim 4 is characterized in that in step 1), and described cleaning stock solution pH is 7.4～8.8, is preferably 8.0, comprises buffering agent, surfactant and stabilizing agent; Buffering agent is phosphate, trishydroxymethylaminomethane or glycocoll, buffering agent shared mass percent in cleaning stock solution is 10～100mmol/L, be preferably 25mmol/L, the mass percent of surfactant and stabilizing agent is respectively 0.1%～0.5% and 0.05%～0.5%, is preferably 0.2% and 0.1%.
9. the preparation method of the turbid kit of particle enhance immunity transmittance of detection adiponectin as claimed in claim 4 is characterized in that in step 2) in, it is that two kinds of latex antibody suspension equal-volumes mix that described two kinds of latex antibody suspensions mix.
10. the preparation method of the turbid kit of particle enhance immunity transmittance of detection adiponectin as claimed in claim 4 is characterized in that in step 3), described buffering agent kind and step 2) in clean that buffering agent is consistent in the stock solution; Described application liquid pH value is 7.4～8.0, is preferably 8.0; The shared mass percent of described surfactant is 0.1%～0.5%, is preferably 0.2%, and the shared mass percent of antiseptic is 0.05%～1%, is preferably 0.1%, and the shared mass percent of macromolecule accelerator is 2%～8%, is preferably 4%; The shared mass percent of sodium chloride is 0.1%～10%, is preferably 1.2%, and the mass percent of buffering agent is 50～200mmol/L, is preferably 100mmol/L.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202066863U (en) * | 2010-10-29 | 2011-12-07 | 厦门大学附属中山医院 | Particle-enhanced turbidimetric immunoassay kit for adiponectin detection |
-
2010
- 2010-10-29 CN CN 201010526950 patent/CN101968492B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202066863U (en) * | 2010-10-29 | 2011-12-07 | 厦门大学附属中山医院 | Particle-enhanced turbidimetric immunoassay kit for adiponectin detection |
Non-Patent Citations (3)
| Title |
|---|
| 李登清、陈琳磊等: "人血清脂联素定量ELISA检测方法的建立及初步临床应用", 《第二军医大学学报》 * |
| 赵满仓、魏文青等: "抗脂联素单克隆抗体的制备及免疫学特性分析", 《华北国防医药》 * |
| 魏文青、赵满仓等: "人血清脂联素酶联免疫吸附试验测定方法的建立及临床应用研究", 《标记免疫分析与临床》 * |
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