CN102879569A - Immunoturbidimetry kit for detecting cysteine proteinase inhibitor C and preparation method thereof - Google Patents
Immunoturbidimetry kit for detecting cysteine proteinase inhibitor C and preparation method thereof Download PDFInfo
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Abstract
The invention relates to an immunoturbidimetry kit for detecting a cysteine proteinase inhibitor C and a preparation method thereof. An application liquid bottle, an antibody suspension liquid bottle and a standard substance bottle are placed in a box body; an application liquid is filled in the application liquid bottle and comprises the following components: 0.01-2% of surfactant, 0.01-1% of preservative, 0.1-20% of sodium chloride and 10-200 mmol/L of buffer solution; a latex suspension liquid coated with a monoclonal antibody of the cysteine proteinase inhibitor C is filled in the antibody suspension liquid bottle and comprises the following components: 0.05-1% of latex coated with the monoclonal antibody of the cysteine proteinase inhibitor C, 0.01-2% of surfactant, 0.01-1% of preservative and 10-200 mmol/L of buffer solution; and a standard substance of the cysteine proteinase inhibitor C is filled in the standard substance bottle. The purpose of large-batch quantitative detection on a biochemical instrument is achieved.
Description
Technical field
The present invention relates to the detection method of immunoturbidimetry, relate in particular to immunoturbidimetry kit that detects cysteine proteinase inhibitor C and preparation method thereof.
Background technology
The cysteine proteinase inhibitor C quick detection kit can be used for human urine, blood plasma and serum and detects.Serum cystatin C be one relatively near the endogenous mark of desirable reflection detection of glomeruli filtration function, research data shows, cysteine proteinase inhibitor C is than serum creatinine (Scr) higher susceptibility and specificity to be arranged, the endogenous mark of comparatively desirable reflection glomerular filtration rate(GFR (GFR).
Application in kidney transplant: in renal transplantation, the GFR of transplanted kidney can be used as one of renal toxicity index of observing acute and chronic rejection and immunodepressant, the functions such as GFR that often can not in time, accurately reflect transplanted kidney by the Scr that slowly raises, Cystatin C then can be used as the good index of monitoring kidney transplant function.
Application in diabetes: blood Cystatin C is a relatively more responsive and practical index that detects diabetic nephropathy, by making regular check on diabetic's Serum Cystatin C, can find in time that GFR changes.
Application in high blood pressure: but the patients with hypertension more than 40% in, late period the Complicated with Hypertension ephrosis.Find according to clinical observation research: hypertension causes renal damage and is divided into three phases: phase one glomerular filtration rate(GFR normal wherein, and intrarenal pressure increases; α-microglobulin etc. appears in the damage of subordinate phase renal tubule high pressure, and the glomerulus earlier damage, and blood Cystatin C raises; The phase III glomerulosclerosis, the renal tubule atrophy, blood Cystatin C obviously raises.So the Serum Cystatin C level of clinical periodic monitoring patients with hypertension can in time be found the Renal function in early period damage that high blood pressure causes, and creates conditions for carrying out early prevention, diagnosis and treatment.
Particle-enhanced turbidimetric immune assay method (particle-enhanced turbidimetric immunoassay, PETIA) be occur in recent years a kind of comparatively stable, body fluid albumen homogeneous phase immunoturbidimetry detection method accurately, its ultimate principle is that antibody falls in surface-crosslinked monoclonal or many grams of certain grain size latex particle, the microballoon of antibody is arranged after antigen is combined when crosslinked, can flock together rapidly at short notice, change the absorbance of reaction system.The concentration of this change and tested antigen has certain correlativity, can reflect the concentration of tested antigen in certain model makes a noise.The mode that this technology strengthens by particle, amplified antigen-antibody reaction, remedied the shortcoming of common turbidimetry insufficient sensitivity, turbidimetry good stability, advantage have easily and fast been inherited again simultaneously, overcome the shortcoming of pricking ELISA and RIA method, more and more be widely used in the clinically quantitative detection of various traces of albumin.A few days ago, the Latex-enhanced immunoturbidimetric assay district is widely used in the detection of LP(a), c reactive protein, antistreptolysin 0, obtained good social benefit, come out but there is not yet so far this type of cysteine proteinase inhibitor C detection kit both at home and abroad.
Summary of the invention
The purpose of this invention is to provide a kind of immunoturbidimetry kit that detects cysteine proteinase inhibitor C and preparation method thereof.
Purpose of the present invention is achieved through the following technical solutions:
Detect the immunoturbidimetry kit of cysteine proteinase inhibitor C, characteristics are: comprise box body, use liquid bottle, antibody suspending liquid bottle and standard items bottle, described application liquid bottle, antibody suspending liquid bottle and standard items bottle are positioned in the box body, in the described application liquid bottle application liquid is housed, component and the percentage by weight thereof of using liquid are: surfactant 0.01% ~ 2%, antiseptic 0.01 ~ 1%, sodium chloride 0.1% ~ 20%, damping fluid 10 ~ 200mmol/L; The latex suspension of coated cysteine proteinase inhibitor C monoclonal antibody is housed in the described antibody suspending liquid bottle, the component of latex suspension and percentage by weight thereof are: the latex 0.05% ~ 0.5% of coated cysteine proteinase inhibitor C monoclonal antibody, surfactant 0.01% ~ 2%, antiseptic 0.01 ~ 1%, damping fluid 10 ~ 200mmol/L; In the described standard items bottle cysteine proteinase inhibitor C standard items are housed.
Further, the immunoturbidimetry kit of above-mentioned detection cysteine proteinase inhibitor C, the surfactant in the described application liquid is Triton-X100, Triton-X405, Tween-20, Tween-40 or Tween-80; Antiseptic in the described application liquid is Sodium azide or Proclin-300 or both potpourris; Damping fluid in the described application liquid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.Surfactant in the described latex suspension is Triton-X100, Triton-X405, Tween-20, Tween-40 or Tween-80; Antiseptic in the described latex suspension is Sodium azide, Proclin-300 or both potpourris; Damping fluid in the described latex suspension is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.
The present invention detects the preparation method of the immunoturbidimetry kit of cysteine proteinase inhibitor C, may further comprise the steps:
A) preparation latex suspension: with latex beads and coated cysteine proteinase inhibitor C monoclonal antibody mixing, 30 ~ 40 degree revolving reactions spend the night, and clean with the cleaning storage liquid and obtain the latex suspension in phosphate buffer, place under 2 ~ 8 degree environment and preserve;
B) Application and preparation liquid: with water-soluble, liquid is applied with surfactant, antiseptic, sodium chloride and damping fluid;
C) preparation cysteine proteinase inhibitor C standard items: select the cysteine proteinase inhibitor C standard items, being diluted to concentration by damping fluid is 8mg/L.
Further, the preparation method of the immunoturbidimetry kit of above-mentioned detection cysteine proteinase inhibitor C, in step a), the particle diameter of latex beads is 100nm ~ 500nm; The pH of described phosphate buffer is 7.2 ~ 9.2, and mass ratio is 20 ~ 200mmol/L; Described cleaning storage liquid pH is 7.4 ~ 9.0, the component and the percentage by weight thereof that clean storage liquid are: surfactant 0.01% ~ 2%, antiseptic 0.01 ~ 1%, damping fluid 10 ~ 200mmol/L, surfactant is Triton-X100, Triton-X405, Tween-20, Tween-40 or Tween-80, antiseptic is Sodium azide, Proclin-300 or both potpourris, and damping fluid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll; It is that equal-volume mixes that described latex beads mixes with coated cysteine proteinase inhibitor C monoclonal antibody.
Further, the preparation method of the immunoturbidimetry kit of above-mentioned detection cysteine proteinase inhibitor C, in the step b), described pH of buffer is 7.4 ~ 9.0, damping fluid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.
Again further, the preparation method of the immunoturbidimetry kit of above-mentioned detection cysteine proteinase inhibitor C, in the step c), described damping fluid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.
The substantive distinguishing features that technical solution of the present invention is outstanding and significant progressive being mainly reflected in:
Mode by the particle enhancing, amplified antigen-antibody reaction, remedied the shortcoming of common turbidimetry insufficient sensitivity, inherited again simultaneously the turbidimetry good stability, advantage easily and fast, overcome the shortcoming of pricking ELISA and RIA method, realize the quantitatively detection in enormous quantities on biochemical instruments of human serum cysteine proteinase inhibitor C, simple to operate, highly sensitive, be not subject to interference from human factor, detect stability and good reproducibility, the content that can truly reflect detected material, can utilize Biochemical Analyzer to detect, easily realize robotization being suitable for clinical application.
Embodiment
Detect the immunoturbidimetry kit of cysteine proteinase inhibitor C, comprise box body, use liquid bottle, antibody suspending liquid bottle and standard items bottle, using liquid bottle, antibody suspending liquid bottle and standard items bottle is positioned in the box body, use in the liquid bottle application liquid is housed, component and the percentage by weight thereof of using liquid are: surfactant 0.01% ~ 2%, antiseptic 0.01 ~ 1%, sodium chloride 0.1% ~ 20%, damping fluid 10 ~ 200mmol/L; The latex suspension of coated cysteine proteinase inhibitor C monoclonal antibody is housed in the antibody suspending liquid bottle, the component of latex suspension and percentage by weight thereof are: the latex 0.05% ~ 0.5% of coated cysteine proteinase inhibitor C monoclonal antibody, surfactant 0.01% ~ 2%, antiseptic 0.01 ~ 1%, damping fluid 10 ~ 200mmol/L; The cysteine proteinase inhibitor C standard items are housed in the standard items bottle.
Wherein, the surfactant of using in the liquid is Triton-X100, Triton-X405, Tween-20, Tween-40 or Tween-80; The antiseptic of using in the liquid is Sodium azide or Proclin-300 or both potpourris; The damping fluid of using in the liquid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.Surfactant in the latex suspension is Triton-X100, Triton-X405, Tween-20, Tween-40 or Tween-80; Antiseptic in the latex suspension is Sodium azide, Proclin-300 or both potpourris; Damping fluid in the latex suspension is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.
The step of preparation process that detects the immunoturbidimetry kit of cysteine proteinase inhibitor C is:
A) preparation latex suspension: with latex beads and coated cysteine proteinase inhibitor C monoclonal antibody mixing, 30 ~ 40 degree revolving reactions spend the night, and clean with the cleaning storage liquid and obtain the latex suspension in phosphate buffer, place under 2 ~ 8 degree environment and preserve; Wherein, the particle diameter of latex beads is 100nm ~ 500nm; The pH of phosphate buffer is 7.2 ~ 9.2, and mass ratio is 20 ~ 200mmol/L; Cleaning storage liquid pH is 7.4 ~ 9.0, the component and the percentage by weight thereof that clean storage liquid are: surfactant 0.01% ~ 2%, antiseptic 0.01 ~ 1%, damping fluid 10 ~ 200mmol/L, surfactant is Triton-X100, Triton-X405, Tween-20, Tween-40 or Tween-80, antiseptic is Sodium azide, Proclin-300 or both potpourris, and damping fluid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll; It is that equal-volume mixes that latex beads mixes with coated cysteine proteinase inhibitor C monoclonal antibody;
B) Application and preparation liquid: with water-soluble, liquid is applied with surfactant, antiseptic, sodium chloride and damping fluid; Wherein, surfactant 0.01% ~ 2%, antiseptic 0.01 ~ 1%, sodium chloride 0.1% ~ 20%, damping fluid 10 ~ 200mmol/L, surfactant are Triton-X100, Triton-X405, Tween-20, Tween-40 or Tween-80, and antiseptic is Sodium azide or Proclin-300 or both potpourris, pH of buffer is 7.4 ~ 9.0, and damping fluid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll;
C) preparation cysteine proteinase inhibitor C standard items: select the cysteine proteinase inhibitor C standard items, being diluted to concentration by damping fluid is 8mg/L; Wherein, damping fluid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.
Embodiment 1
Preparation latex suspension: with latex beads and coated cysteine proteinase inhibitor C monoclonal antibody mixing, 30 ~ 40 degree revolving reactions spend the night, and clean with the cleaning storage liquid and obtain the latex suspension in phosphate buffer, place under 2 ~ 8 degree environment and preserve; Wherein, the particle diameter of latex beads is 120nm; The pH of phosphate buffer is 7.2, and mass ratio is 30mmol/L; Cleaning storage liquid pH is 7.4, and the component and the percentage by weight thereof that clean storage liquid are: surfactant 0.2%, and antiseptic 0.05%, damping fluid 30mmol/L, surfactant are Triton-X405, and antiseptic is Proclin-300, and damping fluid is glycocoll; It is that equal-volume mixes that latex beads mixes with coated cysteine proteinase inhibitor C monoclonal antibody.
Application and preparation liquid: with water-soluble, liquid is applied with surfactant, antiseptic, sodium chloride and damping fluid; Wherein, surfactant 0.1%, antiseptic 0.05%, sodium chloride 5%, damping fluid 30mmol/L, surfactant are Tween-80, and antiseptic is Sodium azide, and pH of buffer is 8.0, and damping fluid is glycocoll.
Preparation cysteine proteinase inhibitor C standard items: select the cysteine proteinase inhibitor C standard items, being diluted to concentration by damping fluid is 8mg/L; Wherein, damping fluid is the amino aminomethane of trihydroxy.
Embodiment 2
Preparation latex suspension: with latex beads and coated cysteine proteinase inhibitor C monoclonal antibody mixing, 30 ~ 40 degree revolving reactions spend the night, and clean with the cleaning storage liquid and obtain the latex suspension in phosphate buffer, place under 2 ~ 8 degree environment and preserve; Wherein, the particle diameter of latex beads is 600nm; The pH of phosphate buffer is 9.2, and mass ratio is 60mmol/L; Cleaning storage liquid pH is 9.0, and the component and the percentage by weight thereof that clean storage liquid are: surfactant 0.4%, antiseptic 0.2%, damping fluid 60mmol/L, surfactant is Triton-X100, and antiseptic is Sodium azide, and damping fluid is the amino aminomethane of trihydroxy; It is that equal-volume mixes that latex beads mixes with coated cysteine proteinase inhibitor C monoclonal antibody.
Application and preparation liquid: with water-soluble, liquid is applied with surfactant, antiseptic, sodium chloride and damping fluid; Wherein, surfactant 0.4%, antiseptic 0.2%, sodium chloride 10%, damping fluid 60mmol/L, surfactant are Tween-20, and antiseptic is Proclin-300, and pH of buffer is 8.0, and damping fluid is the amino aminomethane of trihydroxy.
Preparation cysteine proteinase inhibitor C standard items: select the cysteine proteinase inhibitor C standard items, being diluted to concentration by damping fluid is 8mg/L; Wherein, damping fluid is carbonate.
Embodiment 3
Preparation latex suspension: with latex beads and coated cysteine proteinase inhibitor C monoclonal antibody mixing, 30 ~ 40 degree revolving reactions spend the night, and clean with the cleaning storage liquid and obtain the latex suspension in phosphate buffer, place under 2 ~ 8 degree environment and preserve; Wherein, the particle diameter of latex beads is 180nm; The pH of phosphate buffer is 8.0, and mass ratio is 120mmol/L; Cleaning storage liquid pH is 8.4, and the component and the percentage by weight thereof that clean storage liquid are: surfactant 0.6%, antiseptic 0.4%, damping fluid 120mmol/L, surfactant is Tween-20, and antiseptic is Sodium azide and Proclin-300 potpourri, and damping fluid is carbonate; It is that equal-volume mixes that latex beads mixes with coated cysteine proteinase inhibitor C monoclonal antibody.
Application and preparation liquid: with water-soluble, liquid is applied with surfactant, antiseptic, sodium chloride and damping fluid; Wherein, surfactant 0.6%, antiseptic 0.4%, sodium chloride 15%, damping fluid 120mmol/L, surfactant are Tween-40, and antiseptic is Sodium azide, and pH of buffer is 9.0, and damping fluid is carbonate.
Preparation cysteine proteinase inhibitor C standard items: select the cysteine proteinase inhibitor C standard items, being diluted to concentration by damping fluid is 3.2mg/L; Wherein, damping fluid is the amino aminomethane of trihydroxy.
Embodiment 4
Preparation latex suspension: with latex beads and coated cysteine proteinase inhibitor C monoclonal antibody mixing, 30 ~ 40 degree revolving reactions spend the night, and clean with the cleaning storage liquid and obtain the latex suspension in phosphate buffer, place under 2 ~ 8 degree environment and preserve; Wherein, the particle diameter of latex beads is 200nm; The pH of phosphate buffer is 8.2, and mass ratio is 150mmol/L; Cleaning storage liquid pH is 8.0, and the component and the percentage by weight thereof that clean storage liquid are: surfactant 0.8%, and antiseptic 0.8%, damping fluid 180mmol/L, surfactant are Tween-80, and antiseptic is Sodium azide, and damping fluid is phosphate; It is that equal-volume mixes that latex beads mixes with coated cysteine proteinase inhibitor C monoclonal antibody.
Application and preparation liquid: with water-soluble, liquid is applied with surfactant, antiseptic, sodium chloride and damping fluid; Wherein, surfactant 0.8%, antiseptic 0.8%, sodium chloride 20%, damping fluid 180mmol/L, surfactant are Triton-X100, and antiseptic is Sodium azide and Proclin-300 potpourri, and pH of buffer is 7.4, and damping fluid is phosphate.
Preparation cysteine proteinase inhibitor C standard items: select the cysteine proteinase inhibitor C standard items, being diluted to concentration by damping fluid is 8mg/L; Wherein, damping fluid is glycocoll.
Carry out the actual conditions that cysteine proteinase inhibitor C detects below in conjunction with the immunoturbidimetry kit that adopts described cysteine proteinase inhibitor C at Biochemical Analyzer:
On Hitachi's 7080 biochemical instruments setup parameter:
Temperature of reaction: 37 degree
Analytical approach: 2 end-point methods
Predominant wavelength: 546nm, commplementary wave length 800nm(can not select)
The latex suspension of sample size/application liquid/coated cysteine proteinase inhibitor C antibody: 3ul/240ul/60ul
The Direction of Reaction: rise
Read point is respectively and 31 points at 19
After selecting the multiple spot calibration, instrument is finished calibration automatically, behind the calibration OK, carries out conventional cysteine proteinase inhibitor C and detects.
The mode that the present invention strengthens by particle, amplified antigen-antibody reaction, remedied the shortcoming of common turbidimetry insufficient sensitivity, turbidimetry good stability, advantage have easily and fast been inherited again simultaneously, overcome the shortcoming of pricking ELISA and RIA method, realize the quantitatively detection in enormous quantities on biochemical instruments of human serum cysteine proteinase inhibitor C, the following aspects outstanding advantages is arranged: 1) simple to operate; 2) highly sensitive; 3) be not subject to interference from human factor, detect stability and good reproducibility, can truly reflect the content of detected material; 4) can utilize Biochemical Analyzer to detect, easily realize robotization being suitable for clinical application.
What need to understand is: the above only is preferred implementation of the present invention; for those skilled in the art; under the prerequisite that does not break away from the principle of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. detect the immunoturbidimetry kit of cysteine proteinase inhibitor C, it is characterized in that: comprise box body, use liquid bottle, antibody suspending liquid bottle and standard items bottle, described application liquid bottle, antibody suspending liquid bottle and standard items bottle are positioned in the box body, in the described application liquid bottle application liquid is housed, component and the percentage by weight thereof of using liquid are: surfactant 0.01% ~ 2%, antiseptic 0.01 ~ 1%, sodium chloride 0.1% ~ 20%, damping fluid 10 ~ 200mmol/L; The latex suspension of coated cysteine proteinase inhibitor C monoclonal antibody is housed in the described antibody suspending liquid bottle, the component of latex suspension and percentage by weight thereof are: the latex 0.05% ~ 0.5% of coated cysteine proteinase inhibitor C monoclonal antibody, 0.05% ~ 1%, surfactant 0.01% ~ 2%, antiseptic 0.01 ~ 1%, damping fluid 10 ~ 200mmol/L; In the described standard items bottle cysteine proteinase inhibitor C standard items are housed.
2. the immunoturbidimetry kit of detection cysteine proteinase inhibitor C according to claim 1, it is characterized in that: the surfactant in the described application liquid is Triton-X100, Triton-X405, Tween-20, Tween-40 or Tween-80; Antiseptic in the described application liquid is Sodium azide or Proclin-300 or both potpourris; Damping fluid in the described application liquid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.
3. the immunoturbidimetry kit of detection cysteine proteinase inhibitor C according to claim 1, it is characterized in that: the surfactant in the described latex suspension is Triton-X100, Triton-X405, Tween-20, Tween-40 or Tween-80; Antiseptic in the described latex suspension is Sodium azide, Proclin-300 or both potpourris; Damping fluid in the described latex suspension is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.
4. the preparation method of the immunoturbidimetry kit of detection cysteine proteinase inhibitor C claimed in claim 1 is characterized in that may further comprise the steps:
A) preparation latex suspension: with latex beads and coated cysteine proteinase inhibitor C monoclonal antibody mixing, 30 ~ 40 degree revolving reactions spend the night, and clean with the cleaning storage liquid and obtain the latex suspension in phosphate buffer, place under 2 ~ 8 degree environment and preserve;
B) Application and preparation liquid: with water-soluble, liquid is applied with surfactant, antiseptic, sodium chloride and damping fluid;
C) preparation cysteine proteinase inhibitor C standard items: select the cysteine proteinase inhibitor C standard items, being diluted to concentration by damping fluid is 8mg/L.
5. the preparation method of the immunoturbidimetry kit of detection cysteine proteinase inhibitor C according to claim 4, it is characterized in that: in step a), the particle diameter of latex beads is 120nm ~ 600nm.
6. the preparation method of the immunoturbidimetry kit of detection cysteine proteinase inhibitor C according to claim 4, it is characterized in that: in the step a), the pH of described phosphate buffer is 7.2 ~ 9.2, and mass ratio is 20 ~ 200mmol/L.
7. the preparation method of the immunoturbidimetry kit of detection cysteine proteinase inhibitor C according to claim 4, it is characterized in that: in the step a), described cleaning storage liquid pH is 7.4 ~ 9.0, the component and the percentage by weight thereof that clean storage liquid are: surfactant 0.01% ~ 2%, antiseptic 0.01 ~ 1%, damping fluid 10 ~ 200mmol/L, surfactant is Triton-X100, Triton-X405, Tween-20, Tween-40 or Tween-80, antiseptic is Sodium azide, Proclin-300 or both potpourris, damping fluid are phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.
8. the preparation method of the immunoturbidimetry kit of detection cysteine proteinase inhibitor C according to claim 4, it is characterized in that: in the step a), it is that equal-volume mixes that described latex beads mixes with coated cysteine proteinase inhibitor C monoclonal antibody.
9. the preparation method of the immunoturbidimetry kit of detection cysteine proteinase inhibitor C according to claim 4, it is characterized in that: in the step b), described pH of buffer is 7.4 ~ 9.0, and damping fluid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.
10. the preparation method of the immunoturbidimetry kit of detection cysteine proteinase inhibitor C according to claim 4 is characterized in that: in the step c), described damping fluid is phosphate, carbonate, the amino aminomethane of trihydroxy or glycocoll.
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| CN103217525A (en) * | 2013-03-21 | 2013-07-24 | 上海执诚生物科技股份有限公司 | Composition for improving cystatin C latex coated antibody stability, stabilizer containing the same, preparation method and application thereof |
| CN103454431A (en) * | 2013-09-05 | 2013-12-18 | 苏州照康生物技术有限公司 | Immunoturbidimetric kit for detecting beta2-microglobulin and preparation method thereof |
| CN104049084A (en) * | 2013-03-11 | 2014-09-17 | 南京澳林生物科技有限公司 | Cystine protease inhibitor C detection kit |
| CN104819946A (en) * | 2015-05-07 | 2015-08-05 | 俸家富 | Cystatin C detection kit and detection method of same |
| CN105738617A (en) * | 2016-04-01 | 2016-07-06 | 武汉生之源生物科技股份有限公司 | Cystatin C latex-particle-enhanced turbidimetry detection reagent kit and application thereof |
| CN106645753A (en) * | 2016-12-30 | 2017-05-10 | 广州华弘生物科技有限公司 | Rapid detection kit of beta 2-microglobulin and application of rapid detection kit |
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Application publication date: 20130116 |