CN101954372A - Application of jerusalem artichokes in repairing cadmium (Cd) polluted environment - Google Patents
Application of jerusalem artichokes in repairing cadmium (Cd) polluted environment Download PDFInfo
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- CN101954372A CN101954372A CN 201010281297 CN201010281297A CN101954372A CN 101954372 A CN101954372 A CN 101954372A CN 201010281297 CN201010281297 CN 201010281297 CN 201010281297 A CN201010281297 A CN 201010281297A CN 101954372 A CN101954372 A CN 101954372A
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Abstract
The invention relates to application of jerusalem artichokes in repairing a cadmium (Cd) polluted environment. The repairing method comprises the following steps of: planting the jerusalem artichokes on Cd polluted soil, wherein during seeding, a potassium sulfate compound fertilizer is applied; and when the jerusalem artichokes grow until the end of a flowering phase, integrally moving the roots, stems and leaves of the jerusalem artichoke from the polluted environment to remove Cd in the soil. The jerusalem artichokes can be circularly planted in the polluted soil, wherein under the Cd polluted environment, the concentration of Cd content in overground parts and the root parts of the jerusalem artichokes far exceeds 100mg kg<-1>, which is an established critical content standard required to be reached by hyperaccumulators; under the condition of treatment of 200mg of L<-1>Cd, the Cd content of the overground parts reaches higher than 700mg kg<-1>, and the Cd content of the root parts even reaches higher than 25,000 mg kg<-1>; and when the jerusalem artichokes are planted on soil of which the Cd content is 78mg/kg, the Cd concentration in the roots is 12,285.00mg/kg, the Cd concentration in the stems is 344.53mg/kg, the Cd accumulation quantity in the roots is 1044mg, the Cd accumulation quantity in the stems is 60mg, and the Cd accumulation quantity per mu is 2,568.8g.
Description
One, technical field
The invention belongs to the plant is the pollutant biologic treating technique field of feature, relates in particular to the phytoremediation of environment heavy metal chromium pollution control, and specifically ultraproduct is tired out/application of super enriching plant jerusalem artichoke in restoring cadmium pollution environment.
Two, background technology
In recent years, along with developing rapidly of industrial and agricultural production, heavy metal pollution is serious day by day in the agricultural land soil.China is subjected to nearly 2,000 ten thousand hm of the cultivated area of heavy metal pollution
2, account for 1/5 of cultivated area, wherein cadmium pollution is ploughed and is accounted for 1,133 ten thousand hm
2Large-area contaminated soil like this adopts traditional physics and chemical method all because of reasons such as economy or technology are difficult to carry out, and is in the state of reparation at present substantially.Utilize the excess enrichment of hyperaccumulative plant to have simple to operate with the plant extract recovery technique of removing the heavy metal that exceeds standard in the contaminated soil, economically with technical can large tracts of land enforcement etc. advantage, it is a very promising recovery technique, so the screening of hyperaccumulative plant is the basic research of phytoremediation technology, has very important realistic meaning simultaneously again.
Although utilize hyperaccumulative plant or have a superpower accumulation ability plant a lot as the research of Cd contaminated environment reparation examination material, some super enriching plants have been worked out, but major part is crops, herbaceous plant and water plant, because the biomass of these plants is limited, therefore the absorption and accumulation ability to heavy metal also has certain restriction, and is used for the plant major part of rehabilitating soil and is used for burning to environment and causes certain pollution, so the example that utilizes phytoremediation Cd contaminated soil few report also.
Jerusalem artichoke is the cultigen that can form underground stem tuber in composite family (Compositae) Helianthus, claim foreign ginger, Jerusalem artichoke, Jerusalem artichoke etc. again, formal name used at school Helianthus tuberosus L, in the torrid zone, temperate zone, frigid zone and the arid in the whole world, distribution and the cultivation that all there is jerusalem artichoke the semiarid zone, all can cultivate in 18 provinces and regions such as China northeast, Inner Mongol, Hebei, Henan, Zhejiang, Sichuan, Shandong, Jiangxi, Guangdong, Guangxi, Shaanxi.The jerusalem artichoke happiness is warm, but cold-resistant; Like moistening, but drought-enduring; Like fertile, but impoverishment tolerant, salt tolerant alkali.Stem tuber is emerged for 8～10 ℃ in the germination of sprouting of 6～7 ℃ of eyes, 1～2 ℃ of low temperature of seedling ability, and 18～22 ℃ and help stem tuber at 12 hours at sunshine and form, stem tuber can safe overwintering in-25～-40 ℃ frozen soil layer.Jerusalem artichoke biomass fresh weight reaches 4～8 tons/mu, and wherein aerial part output is 2～3 tons/mu, and underground stem tuber partly is 2～5 tons/mu.Jerusalem artichoke also is used to the anticorrosive protective plant hedge with unstable sand ground of terraced fields; be used to protect the massif soil erosion, fix the sand windproof; its widely purposes and drought-enduring, cold-resistant, anti-saline and alkaline, reproductive capacity strong, the biological characteristics of wide adaptability; make it become the pionner of cadmium pollution environment development and use; have vast potential for future development aspect the bare place resources such as China's cadmium pollution environment utilizing, can obtain bigger economy, society and ecological benefits.
Three, summary of the invention
Goal of the invention: the purpose of this invention is to provide the application process of a kind of jerusalem artichoke in restoring cadmium pollution environment, be adapted at popularizing planting under the cadmium pollution environment, cadmium pollution environment is repaired.Above-mentioned contaminated environment comprises soil pollution and water pollution.
Technical scheme: the application of jerusalem artichoke in restoring cadmium pollution environment.
The application of jerusalem artichoke in restoring cadmium pollution environment, the reparation step is: with the jerusalem artichoke is material, plants on cadmium pollution soil, execute potassium sulfate compound fertilizer during sowing, when jerusalem artichoke length finished to the florescence, jerusalem artichoke root, stem and leaf integral body are removed from contaminated environment, realize the removal of cadmium in the soil.Can in contaminated soil, circulate and plant jerusalem artichoke.
The application of jerusalem artichoke in restoring cadmium pollution environment, the reparation step is: with the jerusalem artichoke stem tuber with eye is material, after washing, carry out surface sterilizing through fungicide, the jerusalem artichoke stem tuber section of bacterium of going out is sowed in the Turnover Box, the Turnover Box bottom is porose and quartz sand is housed, there is 1/2Hoagland ' s (Gram suddenly) nutrient solution in the Turnover Box, nutrient solution is changed every other day, all abundant drip washing quartz sand during each the replacing, natural lighting is cultivated down, treat to use the pouring of 1/2Hoagland ' s nutrient solution after stem tuber is sprouted, carrying out seedling thinning when jerusalem artichoke grows to tri-leaf period transplants, every basin keeps one, treats that jerusalem artichoke grows into six leaves and transplants during the phase to the environment that contains cadmium contaminants and continue to cultivate, when jerusalem artichoke grows when finishing to the florescence, with the jerusalem artichoke root, stem and leaf integral body are removed from contaminated environment, and recirculation is transplanted into the removal of jerusalem artichoke cadmium in realizing environment of six leaf phases.
Beneficial effect: the method that a kind of cadmium pollution environment plant biological provided by the present invention is repaired compared with prior art has following advantage and good effect:
1) jerusalem artichoke is under cadmium pollution environment, and overground part and root cadmium content concentration are far super considerably beyond 100mg kg
-1The critical content standard that this hyperaccumulative plant of generally acknowledging should reach is at 200mg L
-1Cd handles down, and overground part Cd content reaches 700mgkg
-1More than, root Cd content even reach 25000mg kg
-1More than.
2) the jerusalem artichoke biomass is big, and jerusalem artichoke biomass fresh weight reaches 4～8 tons/mu, and wherein aerial part output is 2～3 tons/mu, and underground stem tuber partly is 2～5 tons/mu, and dry weight also can reach 500kg respectively and more than the 250kg.
3) jerusalem artichoke adaptability is strong, the distribution region is wide, can all can cultivate in 18 provinces and regions such as China northeast, Inner Mongol, Hebei, Henan, Zhejiang, Sichuan, Shandong, Jiangxi, Guangdong, Guangxi, Shaanxi in the torrid zone, temperate zone, frigid zone and arid, the semiarid zone plantation in the whole world.
Four, the specific embodiment
Below in conjunction with instantiation, further set forth the present invention.Should be understood that these embodiment only are used for that the present invention will be described, do not constitute the restriction to the claim scope, other alternative means that it may occur to persons skilled in the art that are all in claim scope of the present invention.
1/2Hoagland ' s (Gram suddenly) nutrient solution prescription: 2.5mM Ca (NO
3)
2, 2.5mM KNO
3, 0.5mMKH
2PO
4, 0.5mM MgSO
4, 25 μ M H
3BO
3, 2.25 μ M MnCl
2, 1.9 μ M ZnSO
4, 0.15 μ M CuSO
4, 0.05mM (NH
4)
6Mo
7O
24, 5 μ M FeEDTA.
Used jerusalem artichoke is southern taro No. 1 among the embodiment, and nutrient solution is: 1/2Hoagland ' s (Gram suddenly) nutrient solution; Potassium sulfate compound fertilizer (15-15-15 type): the source fertile industry of Shandong Xu Yang Co., Ltd.
Embodiment 1:
With jerusalem artichoke (southern taro No. 1) is material, the jerusalem artichoke stem tuber with running water and distilled water flushing clean after, carry out surface sterilizing through 0.05% fungicide.The jerusalem artichoke stem tuber section with eye of bacterium of going out is sowed at and places Turnover Box that the porose plastic tub alms bowl of quartz sand and bottom is housed, Turnover Box has 1/2Hoagland ' s (Gram suddenly) nutrient solution of certain depth, change nutrient solution every other day, all abundant at every turn drip washing quartz sand, to guarantee the homogeneity of condition of culture, natural lighting.Treat to use 1/2Hoagland ' s nutrient solution (2.5mM Ca (NO after stem tuber is sprouted
3)
2, 2.5mM KNO
3, 0.5mM KH
2PO
4, 0.5mM MgSO
4, 25 μ MH
3BO
3, 2.25 μ M MnCl
2, 1.9 μ M ZnSO
4, 0.15 μ M CuSO
4, 0.05mM (NH
4)
6Mo
7O
24, 5 μ MFeEDTA, down together) pouring.Carry out seedling thinning during to tri-leaf period and transplant, every basin keeps one, and each handles three basins.Treat that six leaves handle during the phase, 5mg L is set
-1Cd concentration, during processing directly with the CdCl of solid forms
22.5H
2O is dissolved in 1/2Hoagland ' the s nutrient solution, and fully stirring and evenly mixing waters.Change nutrient solution during the processing every other day, should note during replacing not allowing nutrient solution be stained with above-ground plant parts, handle three all post-samplings and detect.The result is as follows: the long 8.13cm of leaf, the wide 3.27cm of leaf, plant height 22.27cm, the long 17.93cm of root, root dry weight 0.26g, ground dry weight 0.68g, complete stool dry weight 0.94g, Cd concentration 1089.90mg/kg in the root, Cd concentration 121.82mg/kg in the stem, Cd concentration 87.44mg/kg in the leaf, root Cd accumulation 0.28mg, stem Cd accumulation 0.08mg, leaf Cd accumulation 0.04mg.
Embodiment 2:
With jerusalem artichoke (southern taro No. 1) is material, and condition of culture is the same, treats that six leaves handle during the phase, and 25mg L is set
-1Cd concentration, during processing directly with the CdCl of solid forms
22.5H
2O is dissolved in 1/2Hoagland ' the s nutrient solution, and fully stirring and evenly mixing waters.Change 1/2Hoagland ' s (Gram suddenly) nutrient solution during the processing every other day, should note during replacing not allowing nutrient solution be stained with above-ground plant parts, handle three all post-samplings and detect.The result is as follows: the long 7.47cm of leaf, the wide 3.13cm of leaf, plant height 15.90cm, the long 12.37cm of root, root dry weight 0.18g, ground dry weight 0.51g, complete stool dry weight 0.69g, Cd concentration 1344.70mg/kg in the root, Cd concentration 174.79mg/kg in the stem, Cd concentration 141.90mg/kg in the leaf, root Cd accumulation 0.24mg, stem Cd accumulation 0.09mg, leaf Cd accumulation 0.04mg.
Embodiment 3:
With jerusalem artichoke (southern taro No. 1) is material, and condition of culture is the same, treats that six leaves handle during the phase, and 50mg L is set
-1Cd concentration, during processing directly with the CdCl of solid forms
22.5H
2O is dissolved in 1/2Hoagland ' the s nutrient solution, and fully stirring and evenly mixing waters.Change 1/2Hoagland ' s (Gram suddenly) nutrient solution during the processing every other day, should note during replacing not allowing nutrient solution be stained with above-ground plant parts, handle three all post-samplings and detect.The result is as follows: the long 7.80cm of leaf, the wide 2.80cm of leaf, plant height 15.77cm, the long 11.93cm of root, root dry weight 0.22g, ground dry weight 0.64g, complete stool dry weight 0.86, Cd concentration 2167.90mg/kg in the root, Cd concentration 331.20mg/kg in the stem, Cd concentration 328.77mg/kg in the leaf, root Cd accumulation 0.48mg, stem Cd accumulation 0.21mg, leaf Cd accumulation 0.11mg.
Embodiment 4:
With jerusalem artichoke (southern taro No. 1) is material, and condition of culture is the same, treats that six leaves handle during the phase, and 100mg L is set
-1Cd concentration, during processing directly with the CdCl of solid forms
22.5H
2O is dissolved in 1/2Hoagland ' the s nutrient solution, and fully stirring and evenly mixing waters.Change 1/2Hoagland ' s (Gram suddenly) nutrient solution during the processing every other day, should note during replacing not allowing nutrient solution be stained with above-ground plant parts, handle three all post-samplings and detect.The result is as follows: the long 6.67cm of leaf, the wide 2.57cm of leaf, plant height 14.30cm, the long 11.17cm of root, root dry weight 0.15g, ground dry weight 0.53g, complete stool dry weight 0.68, Cd concentration 6090.80mg/kg in the root, Cd concentration 404.59mg/kg in the stem, Cd concentration 303.38mg/kg in the leaf, root Cd accumulation 0.91mg, stem Cd accumulation 0.22mg, leaf Cd accumulation 0.08mg.
Embodiment 5:
With jerusalem artichoke (southern taro No. 1) is material, and condition of culture is the same, treats that six leaves handle during the phase, and 200mg L is set
-1Cd concentration, during processing directly with the CdCl of solid forms
22.5H
2O is dissolved in 1/2Hoagland ' the s nutrient solution, and fully stirring and evenly mixing waters.Change 1/2Hoagland ' s (Gram suddenly) nutrient solution during the processing every other day, should note during replacing not allowing nutrient solution be stained with above-ground plant parts, handle three all post-samplings and detect.The result is as follows: the long 6.63cm of leaf, the wide 2.23cm of leaf, plant height 12.03cm, the long 9.87cm of root, root dry weight 0.10g, ground dry weight 0.34g, complete stool dry weight 0.44, Cd concentration 25105.00mg/kg in the root, Cd concentration 743.85mg/kg in the stem, Cd concentration 288.55mg/kg in the leaf, root Cd accumulation 2.51mg, stem Cd accumulation 0.25mg, leaf Cd accumulation 0.05mg.
Embodiment 6:
With jerusalem artichoke (southern taro No. 1) is material, and condition of culture is the same, treats that jerusalem artichoke grows into six leaves and transplants during the phase to the environment that contains cadmium contaminants, and soil cadmium content 78mg/kg executes potassium sulfate compound fertilizer (15-15-15 type) 600kg/hm during sowing
2, mu plantation 3780 strains, growing period does not refertilize, and only intertill and clean tillage when jerusalem artichoke length finished to the florescence, is removed jerusalem artichoke root, stem and leaf integral body from contaminated environment.The result is as follows: the average 194cm of plant height, the average thick 22.8mm of stem, the on average heavy 85g of individual plant root, the average dry weight 174g in ground, Cd concentration 12285.00mg/kg in the root, Cd concentration 344.53mg/kg in the stem, root Cd accumulation 1044mg, stem Cd accumulation 60mg, mu remove Cd amount 2568.8g.
Claims (4)
1. the application of jerusalem artichoke in restoring cadmium pollution environment.
2. the application of jerusalem artichoke in restoring cadmium pollution environment, it is characterized in that repairing step is: be material with the jerusalem artichoke, on cadmium pollution soil, plant, execute potassium sulfate compound fertilizer during sowing, when jerusalem artichoke length finished to the florescence, jerusalem artichoke root, stem and leaf integral body are removed from contaminated environment, realized the removal of cadmium in the soil.
3. the application of jerusalem artichoke according to claim 2 in restoring cadmium pollution environment is characterized in that circulation plantation jerusalem artichoke in contaminated soil.
4. the application of jerusalem artichoke in restoring cadmium pollution environment, it is characterized in that repairing step is: with the jerusalem artichoke stem tuber with eye is material, after washing, carry out surface sterilizing through fungicide, the jerusalem artichoke stem tuber section of bacterium of going out is sowed in the Turnover Box, the Turnover Box bottom is porose and quartz sand is housed, there is 1/2Hoagland ' s nutrient solution in the Turnover Box, nutrient solution is changed every other day, all abundant drip washing quartz sand during each the replacing, natural lighting is cultivated down, treat to use the pouring of 1/2Hoagland ' s nutrient solution after stem tuber is sprouted, carrying out seedling thinning when jerusalem artichoke grows to tri-leaf period transplants, every basin keeps one, treats that jerusalem artichoke grows into six leaves and transplants during the phase to the environment that contains cadmium contaminants and continue to cultivate, when jerusalem artichoke grows when finishing to the florescence, with the jerusalem artichoke root, stem and leaf integral body are removed from contaminated environment, and recirculation is transplanted into the removal of jerusalem artichoke cadmium in realizing environment of six leaf phases.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102277502A (en) * | 2011-04-28 | 2011-12-14 | 彭克俭 | Method for enrichment extraction of cadmium in polluted soil through herbaceous plant Chinese lobelia herb |
| RU2590805C1 (en) * | 2015-04-28 | 2016-07-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Тверская государственная сельскохозяйственная академия" (ФГБОУ ВПО "Тверская государственная сельскохозяйственная академия") | Method for making girasol products for specific goals |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101386018A (en) * | 2008-10-22 | 2009-03-18 | 辽宁大学 | Cadmium pollution soil repair method using compositae |
| CN101693252A (en) * | 2009-10-30 | 2010-04-14 | 四川农业大学 | Application of siegesbeckia herb in remediation of soil contaminated with heavy metal cadmium |
-
2010
- 2010-09-14 CN CN 201010281297 patent/CN101954372A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101386018A (en) * | 2008-10-22 | 2009-03-18 | 辽宁大学 | Cadmium pollution soil repair method using compositae |
| CN101693252A (en) * | 2009-10-30 | 2010-04-14 | 四川农业大学 | Application of siegesbeckia herb in remediation of soil contaminated with heavy metal cadmium |
Non-Patent Citations (1)
| Title |
|---|
| 《植物生理与分子生物学》 20070430 陶毅明 镉诱导的菊芋幼苗膜脂过氧化和抗氧化酶活性及过氧化物酶同工酶的改变 第301页左栏第1行-第308页右栏最后1行 1-4 , 2 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102277502A (en) * | 2011-04-28 | 2011-12-14 | 彭克俭 | Method for enrichment extraction of cadmium in polluted soil through herbaceous plant Chinese lobelia herb |
| RU2590805C1 (en) * | 2015-04-28 | 2016-07-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Тверская государственная сельскохозяйственная академия" (ФГБОУ ВПО "Тверская государственная сельскохозяйственная академия") | Method for making girasol products for specific goals |
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Open date: 20110126 |