CN101926452A - Wild jujube leaf total flavonoids extract and preparation method thereof - Google Patents
Wild jujube leaf total flavonoids extract and preparation method thereof Download PDFInfo
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- CN101926452A CN101926452A CN2009102602103A CN200910260210A CN101926452A CN 101926452 A CN101926452 A CN 101926452A CN 2009102602103 A CN2009102602103 A CN 2009102602103A CN 200910260210 A CN200910260210 A CN 200910260210A CN 101926452 A CN101926452 A CN 101926452A
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Abstract
The invention relates to a wild jujube leaf total flavonoids extract and a preparation method thereof. The preparation method comprises the following steps of extracting a concentrated total flavonoids extract from wild jujube leaves by using an ethyl alcohol extracting method, adopting an ethanol solvent with a certain concentration in the extraction process, obtaining an optical extraction condition by optimizing factors such as the extraction time, the extraction temperature, the solid-liquid ratio and the like, preparing a wild jujube leaf flavonoids extract liquid, and filtering and concentrating the prepared extract liquid to finally obtain the wild jujube leaf total flavonoids extract. The yield of the crude extract is not less than 60 percent, and a paste is rich in various nutritional components and comprises the main components: total flavonoids of no less than 9 percent, protein of no less than 5 percent, total sugar of no less than 4 percent and 1 percent of tannin. The invention has the advantages of simple preparation technology and convenient operation and is suitable for scale industrial production.
Description
Technical field
The present invention relates to a kind of extraction process of utilizing the Wild jujube leaf to prepare flavones medicinal extract, belong to Food Science and technical field.
Background technology
Flavone compound is a class secondary metabolism class material that extensively exists in the higher plant, has very strong removing free radical ability, it is one of main active of medicinal plant, relevant pharmacology and clinical trial confirm, flavone compound has pharmacological function widely, as all containing flavone compound in effective Chinese herbal medicine of many treatment coronary heart disease or the blood-activating stasis-removing kind Chinese medicine.Because this compounds has diversified biologically active and pharmacological function, and toxicity is lower, enjoys people to pay attention to, become the emphasis that the functional food research and development utilizes.Flavonoid substances is present in the fruit, flower, blade of plant widely, and blade is big at the occurring in nature amount, wide material sources, therefore from plant leaf blade, extract Flavonoid substances and obtained development widely in recent years, such as publication number is CN 101224224A, application number 200810010012.7 " extracts the method for crude flavonoid and flavone glycoside " in the ginkgo leaf, be to be that raw material extracts Flavonoid substances with the ginkgo leaf; Publication number is CN 1597678A, and application number 03133957.3 " extracting method of haw thorn leaf total flavone " is to be that raw material extracts Flavonoid substances with the leaves of Hawthorn, does not still have the report that wild jujube leaf flavones extracts research.
Wild jujube has another name called the mountain jujube, is grown in the perennial shrub of the northern area of China.In northern China larger area Wild jujube woods is arranged, resource is very abundant.The flowers and fruits of wild jujube, branches and leaves and kind benevolence all have the pharmacology nutritive value, and is at present more abundant for the research of spina date seed flavones, and still belong to the blank stage for the development and use of jujube leaf.
The present invention adopts solvent that Wild jujube leaf total flavonoids is carried out extraction and determination; be intended to find the proper method of general flavone in the extraction wild jujube leaf; for the deep development utilization of wild jujube product provides scientific and effective, as to be convenient to a large-scale production preparation technology, also provide new approach simultaneously for the comprehensive development and utilization of hill resource.
Summary of the invention
The objective of the invention is to utilize the Wild jujube leaf, optimize extraction conditions, thereby obtain the Wild jujube leaf total flavonoids extract of high level, for more vast market prospect is opened up in the industrialization development of wild jujube Related product.
Process chart is seen Figure of description, and detailed process is as follows:
(1) wild jujube leaf drying and crushing
Get 105 ℃ of 15min that complete of Wild jujube leaf, 65 ℃ of aeration-dryings are pulverized to constant weight.
(2) degreasing wild jujube leaf powder preparation
With the wild jujube leaf powder of drying and crushing, with the benzinum backflow lixiviate 1.5h degreasing of 5 times of volumes of its quality, the wild jujube leaf powder after the degreasing is carried out desolventizing, obtain degreasing wild jujube leaf powder.
(2) wild jujube leaf CE preparation
Take by weighing a certain amount of degreasing wild jujube leaf powder, adopting volume ratio is 50-80% alcohol solvent lixiviate 1-3h, extraction temperature 50-80 ℃, and solid-liquid ratio 1: 30-1: 60.After extraction is finished, filter and to discard precipitation, merge extract, and vacuum decompression concentrates under 60 ℃ the condition in being lower than, soluble solid reaches at 40% o'clock in the thing to be extracted, obtains paste concentrate (also it can be carried out obtaining the thick product of powdery after the vacuum drying).In addition, also can make with extra care, obtain the higher wild jujube leaf flavones goods of purity with macroporous absorbent resin.
(3) product the key technical indexes
Product is pale brown look paste, and is slightly bitter.Wild jujube leaf total flavonoids extract yield 〉=60%, general flavone content 〉=9%, general flavone recovery rate 〉=78%, protein content 〉=5%, total sugar content 〉=4%, tannin content 1%.
(4) detection method
Adopt alkaline NaNO
2-Al (NO
3)
3Development process is that standard items are measured the sample light absorption value in wavelength 510nm place with the rutin, calculates Wild jujube leaf total flavonoids content according to calibration curve.
The specific embodiment
The present invention is described in further detail by following examples, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
The wild jujube leaf of drying is ground into powder,, the wild jujube leaf powder after the degreasing is carried out desolventizing, obtain degreasing wild jujube leaf powder with the benzinum backflow lixiviate 1.5h degreasing of 5 times of volumes of its quality.Take by weighing the degreasing wild jujube leaf powder art of quantitative drying, add the ethanolic solution of 60% (volume ratio), extract 1.5h in 67 ℃ of water-baths, the ethanol consumption is solid-liquid ratio 1: 50.Extraction is filtered after finishing, and collects merging filtrate.With the centrifugal removal of impurities of above-mentioned wild jujube leaf extract, it is stand-by to collect supernatant.Supernatant is carried out vacuum decompression concentrate, reclaim ethanol.Thickening temperature is 60 ℃, vacuum-0.1～-0.06MPa, reach 40% until the concentrate soluble solid and end.(also the freeze drying of paste concentrate can be pulverized, makes Powdered wild jujube leaf flavonoids crude extract).Its yield is 61.5%, and Wild jujube leaf total flavonoids content is 9.1% in the paste, protein 5.5%, total reducing sugar 4.3%, tannin 1%.
Claims (2)
1. wild jujube leaf flavones extract making method is characterized in that comprising following preparation process:
(1) get the Wild jujube leaf and complete, aeration-drying is at a certain temperature pulverized to constant weight, and degreasing is carried out desolventizing with the wild jujube leaf powder after the degreasing, obtains degreasing wild jujube leaf powder;
(2) with certain density ethanol lixiviate step (1) wild jujube leaf powder, extract the pure soluble components in the wild jujube leaf, obtain extract, " above-mentioned extracting technology can be taked any method in percolation, single-stage infusion process, multistage infusion process, continuous flow upstream extraction and the circumfluence method ";
(3) extract that obtains of step (2) concentrates and reclaims ethanol and make wild jujube leaf flavones medicinal extract, wherein general flavone content 〉=9%.
2. according to the described wild jujube leaf of claim 1 flavones extract making method, it is characterized in that:
Step (1) condition of completing is temperature 80-120 ℃, and fixation time 10-30min, baking temperature are 65 ℃, and degreasing solvent is benzinum, these degreasing solvents commonly used of solvent naphtha;
The concentration of alcohol that adopts during step (2) lixiviate is 50-80% (volume ratio), and extraction time is 1-3h, and extraction temperature 50-80 ℃, solid-liquid ratio is 1: 30-1: 60.
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| CN2009102602103A CN101926452B (en) | 2009-12-25 | 2009-12-25 | Wild jujube leaf total flavonoids extract and preparation method thereof |
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| CN101926452A true CN101926452A (en) | 2010-12-29 |
| CN101926452B CN101926452B (en) | 2012-12-05 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104547104A (en) * | 2013-10-24 | 2015-04-29 | 沈阳药科大学 | Wild jujube stem and leaf extract, as well as preparation method and application thereof |
| CN105232669A (en) * | 2015-11-05 | 2016-01-13 | 山西大学 | Wild jujube leaf flavone extract, and preparation method and application thereof |
| CN105884842A (en) * | 2016-01-28 | 2016-08-24 | 塔里木大学 | Quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside prepared from red date leaves and application thereof |
| CN107951936A (en) * | 2017-11-16 | 2018-04-24 | 金华市飞凌生物科技有限公司 | There is the wild jujube extract for improving sleep |
| CN108419870A (en) * | 2018-03-15 | 2018-08-21 | 洋县鹮居花茶产业发展有限公司 | A kind of preparation method of chrysanthemum tea |
| CN110801014A (en) * | 2019-11-29 | 2020-02-18 | 恩施硒禾生物科技有限公司 | Method for preparing selenium-rich health food by using kudzu leaf extract |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1175758C (en) * | 2002-09-25 | 2004-11-17 | 河北农业大学 | Separating and extracting method for date cyclic nucleotide syrup, dietary fibre and date wax |
-
2009
- 2009-12-25 CN CN2009102602103A patent/CN101926452B/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104547104A (en) * | 2013-10-24 | 2015-04-29 | 沈阳药科大学 | Wild jujube stem and leaf extract, as well as preparation method and application thereof |
| CN104547104B (en) * | 2013-10-24 | 2017-09-08 | 沈阳药科大学 | Ramulus Ziziphi Spinosae leaf extract and its preparation method and application |
| CN105232669A (en) * | 2015-11-05 | 2016-01-13 | 山西大学 | Wild jujube leaf flavone extract, and preparation method and application thereof |
| CN105232669B (en) * | 2015-11-05 | 2019-05-17 | 山西大学 | A kind of wild jujube leaf flavone extract and its preparation method and application |
| CN105884842A (en) * | 2016-01-28 | 2016-08-24 | 塔里木大学 | Quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside prepared from red date leaves and application thereof |
| CN105884842B (en) * | 2016-01-28 | 2021-03-12 | 塔里木大学 | Preparation of quercetin-3-O-alpha-L-arabinose- (1 → 2) -alpha-L-rhamnoside from red date leaves and application thereof |
| CN107951936A (en) * | 2017-11-16 | 2018-04-24 | 金华市飞凌生物科技有限公司 | There is the wild jujube extract for improving sleep |
| CN108419870A (en) * | 2018-03-15 | 2018-08-21 | 洋县鹮居花茶产业发展有限公司 | A kind of preparation method of chrysanthemum tea |
| CN110801014A (en) * | 2019-11-29 | 2020-02-18 | 恩施硒禾生物科技有限公司 | Method for preparing selenium-rich health food by using kudzu leaf extract |
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| CN101926452B (en) | 2012-12-05 |
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