CN101899483B

CN101899483B - Method for reducing ammonia-N content in L-valine fermented waste fluid

Info

Publication number: CN101899483B
Application number: CN201010244448XA
Authority: CN
Inventors: 宁健飞; 蔡立明
Original assignee: WUXI JINGHAI AMINO ACID CO Ltd
Current assignee: WUXI JINGHAI AMINO ACID CO., LTD.
Priority date: 2010-07-28
Filing date: 2010-07-28
Publication date: 2011-07-27
Anticipated expiration: 2030-07-28
Also published as: CN101899483A

Abstract

The invention provides a method for reducing ammonia-N content in L-valine fermented waste fluid, in particular to a clean producing method for L-valine, belonging to the technical field of biochemical engineering. In the method, in the L-valine fermenting process, sodium hydroxide solution partially replaces fed-match ammonia to supply nitrogen source and control pH, while the yield of the L-valine is not reduced, and the dosage of the ammonia and the content of the ammonia-N in the fermented waste fluid are reduced. Compared with the prior art, the method has the advantages of greatly reducing the content of the ammonia-N in the fermented waste fluid to ensure that the content of the ammonia-N in the L-valine fermented waste fluid is reduced by over 80 percent and the dosage of the ammonia is reduced by over 50 percent.

Description

A kind of method that reduces ammonium nitrogen content in the L-Xie Ansuan fermented waste fluid

Technical field

A kind of method that reduces ammonium nitrogen content in the L-Xie Ansuan fermented waste fluid is specifically related to a kind of clean preparation method of L-Xie Ansuan, belongs to technical field of biochemical industry.

Background technology

The L-Xie Ansuan is one of eight kinds of indispensable amino acids of human body, is again one of three kinds of branched-chain amino acids simultaneously, has application and commercial value widely at medicine and food service industry, and the L-Xie Ansuan mainly adopts fermentative Production at present.In the fermenting process of L-Xie Ansuan, need intermittent flow to add ammoniacal liquor and be used to regulate pH and additional nitrogenous source.Adopt existing explained hereafter L-Xie Ansuan, ammonium nitrogen content is higher in the fermented waste fluid.The processing that contains ammonium nitrogen fermented waste fluid at present all is adopt " an end treatment technology ", reaches the technological standard of environmental sound in the hope of the discharging waste liquid.The treatment process of this fermented waste fluid is a passiveness, and the contamination governing method of curing the symptoms, not the disease particularly to a large amount of industry high-concentration waste water, is difficult to radical cure; Say from technical standpoint, the development difficulty of end treatment technology is not second to the exploitation of production technology, and bigger problem is economic problems, a small amount of output of end treatment technology, be difficult to offset the fund input of engineering construction and the working cost of daily costliness, big more being difficult to more of industrial scale bears.And cleaner production is emphasized at the source reduction refuse, production process is not produced or produces refuse to greatest extent less.Therefore in the fermentation production process of L-Xie Ansuan, make the content of ammonium nitrogen in the consumption of ammoniacal liquor and the fermented waste fluid that reduction in various degree all be arranged as adopting the production technique of cleaning comparatively, will bring remarkable economic efficiency and social benefit.

Summary of the invention

The objective of the invention is to: the method for ammonium nitrogen content in a kind of L-of reduction Xie Ansuan fermented waste fluid is provided, and this method can be able to make the content of ammonium nitrogen in the L-Xie Ansuan fermented waste fluid reduce more than 80%.

In the fermenting process of L-Xie Ansuan, need stream to add ammoniacal liquor to regulate pH and additional nitrogenous source.But because the ammoniacal liquor of fermentation middle and later periods only plays the effect of regulating pH, in time do not utilized, cause in the fermented waste fluid ammonium nitrogen content higher.The invention provides the method for ammonium nitrogen content in a kind of L-of reduction Xie Ansuan fermented waste fluid, its principle is in the fermentation middle and later periods, stops stream and adds ammoniacal liquor, changes stream hydro-oxidation sodium solution into and regulates pH, to reach the purpose that reduces ammonium nitrogen content in the fermented waste fluid.

The technical recipe and the processing parameter of the fermentation technique of existing L-Xie Ansuan are summarised as table 1.

Table 1 technical recipe and processing parameter

Fermented bacterium is brevibacterium flavum JHV 2-58, [the scientific and technological achievement assay certificate] that this bacterial strain has been called " gordian technique and the industrialization of branched-chain amino acid high yield bacterium process for cleanly preparing " in the name of Wuxi City science and technology office tissue characterization (2009-1-17) go up open.On the basis of above-mentioned technical recipe, cultivate, shake a bottle one-level kind receive seeding tank expand join after, insert and produce fermentor tank, in the culturing process of fermentor tank, by the suitable pH value of stream ammonification water management, the fermentation culture of assurance bacterial classification.The production process initial flow adds ammoniacal liquor for inoculating back 3-25 hour, and whole stream added the cycle at 30-65 hour, and after the fermentation ends, the remaining ammonia nitrogen of fermented liquid is at 3000-6000mg/L.

Technical scheme of the present invention: a kind of method that reduces ammonium nitrogen content in the L-Xie Ansuan fermented waste fluid, in the fermenting process of L-Xie Ansuan, the ammoniacal liquor that partly replaces stream to add with sodium hydroxide solution is to reduce the content of ammonium nitrogen in the fermented waste fluid; Processing condition are:

Fermented liquid inoculation 3-25 hour, preferred after 4-15 hour, begin stream and add ammoniacal liquor to replenish nitrogenous source and control pH6.8-7.5, it is 15-40 hour that stream adds the cycle, preferred 20-40 hour, the ammoniacal liquor mass concentration that stream adds was 50%-100%, preferred 70%-100%.

From fermented liquid inoculation 20-45 hour, preferred after 25-45 hour, stop stream and add ammoniacal liquor, change stream hydro-oxidation sodium solution control pH6.8-7.5 into, it is 10-40 hour that stream adds the cycle, and preferred streams adds cycle 15-25 hour, the concentration of sodium hydroxide solution that stream adds is 0.1-5mol/L, preferred 0.5-2mol/L.

Beneficial effect of the present invention: the present invention compares with existing Technology, and the superiority of the inventive method is the ammonium nitrogen content that can reduce significantly in the fermented waste fluid, makes the content of ammonium nitrogen in the L-Xie Ansuan fermented waste fluid reduce more than 80%; Make ammonia volume reduce more than 50%.

Embodiment

Embodiment 1

The 3.0 liters of fermented liquids of packing in 5 liters the fermentor tank, inoculate 5% seed liquor, ferment and flow after 6 hours between the ammonification water management pH to 6.8-7.5, ferment and stopped stream in 38 hours and add ammoniacal liquor, change between the stream hydro-oxidation sodium solution control pH to 6.8-7.5, during to fermentation ends, the output of L-Xie Ansuan is 42.4g/L.Compare with existing Technology, ammonia volume reduces about 50%, and the content of ammonium nitrogen is reduced to about 700mg/L from about 4000mg/L in the fermented liquid.

Embodiment 2

The 300 liters of fermented liquids of packing in 500 liters the fermentor tank, inoculate 5% seed liquor, ferment and flow after 10 hours between the ammonification water management pH to 6.8-7.5, ferment and stopped stream in 40 hours and add ammoniacal liquor, change between the stream hydro-oxidation sodium control pH to 6.8-7.5, during to fermentation ends, the output of L-Xie Ansuan is 43.7g/L.Compare with existing Technology, ammonia volume reduces about 50%, and the content of ammonium nitrogen is reduced to about 500mg/L from 4000mg/L in the fermented liquid.

Embodiment 3

The 3000 liters of fermented liquids of packing in 5000 liters the fermentor tank, inoculate 5% seed liquor, ferment and flow after 5 hours between the ammonification water management pH to 6.8-7.5, ferment and stopped stream in 39 hours and add ammoniacal liquor, change between the stream hydro-oxidation sodium control pH to 6.8-7.5, during to fermentation ends, the output of L-Xie Ansuan is 42.7g/L.Compare with existing Technology, ammonia volume reduces about 50%, and the content of ammonium nitrogen is reduced to about 700mg/L from 4000mg/L in the fermented liquid.

Embodiment 4

The 30000 liters of fermented liquids of packing in 50000 liters the fermentor tank, inoculate 5% seed liquor, stream ammonification water management pH to 6.8-7.5 after 6 hours ferments, ferment and stopped stream in 38 hours and add ammoniacal liquor, change stream hydro-oxidation sodium solution control pH to 6.8-7.5 into, during to fermentation ends, the output of L-Xie Ansuan is 43.4g/L.Compare with existing Technology, ammonia volume reduces about 50%, and the content of ammonium nitrogen is reduced to about 600mg/L from 4000mg/L in the fermented liquid.

Claims

1. a method that reduces ammonium nitrogen content in the L-Xie Ansuan fermented waste fluid is characterized in that in the fermenting process of L-Xie Ansuan, and the ammoniacal liquor that partly replaces stream to add with sodium hydroxide solution is to reduce the content of ammonium nitrogen in the fermented waste fluid; Processing condition are:

The fermented liquid inoculation began stream and adds ammoniacal liquor to replenish nitrogenous source and control pH6.8-7.5 after 3-25 hour, and it is 15-40 hour that stream adds the cycle, and the ammoniacal liquor mass concentration that stream adds is 50%-100%;

, stop stream and add ammoniacal liquor after 20-45 hour from the fermented liquid inoculation, change stream hydro-oxidation sodium solution control pH6.8-7.5 into, it is 10-40 hour that stream adds the cycle, and the concentration of sodium hydroxide solution that stream adds is 0.1-5mol/L.

2. the method for ammonium nitrogen content is characterized in that in the fermenting process of L-Xie Ansuan in the reduction according to claim 1 L-Xie Ansuan fermented waste fluid, and the ammoniacal liquor that partly replaces stream to add with sodium hydroxide solution is to reduce the content of ammonium nitrogen in the fermented waste fluid; Processing condition are:

The fermented liquid inoculation began stream and adds ammoniacal liquor to replenish nitrogenous source and control pH6.8-7.5 after 4-15 hour, and it is 20-40 hour that stream adds the cycle, and the ammonia concn that stream adds is 70%-100%;

, stop stream and add ammoniacal liquor after 25-45 hour from the fermented liquid inoculation, change stream hydro-oxidation sodium solution control pH6.8-7.5 into, it is 15-25 hour that stream adds the cycle, and the concentration of sodium hydroxide solution that stream adds is 0.5-2mol/L.