CN101893578A - Method for automatically detecting urinary sediment and microscopic detection device - Google Patents
Method for automatically detecting urinary sediment and microscopic detection device Download PDFInfo
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- CN101893578A CN101893578A CN 201010216805 CN201010216805A CN101893578A CN 101893578 A CN101893578 A CN 101893578A CN 201010216805 CN201010216805 CN 201010216805 CN 201010216805 A CN201010216805 A CN 201010216805A CN 101893578 A CN101893578 A CN 101893578A
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Abstract
The invention provides a method for automatically detecting urinary sediment. In the method, a great number of 40X picture are only continuously acquired and analyzed in the automatic detection of the urinary sediment in a microscopy mode. In the analysis process, the pictures are all screened and a small number of pictures are subjected to classification identification so as to ensure the analysis speed. Meanwhile, a microscopic device used for the detection is a microscopic device which has the advantages of capacities of greatly simplifying the structure and removing the high-low power mirror changing function and Z-direction automatic focusing function, along with convenient design and small and simple structure. The invention also provides a microscopic detection device, which is only provided with a 40X objective lens and does not have the Z-direction automatic focusing function. An XY-direction motion platform substrate and a microscopic device substrate are fixed together for reducing the influence of platform shake on picture acquisition during scanning and acquiring the pictures. Initial focusing can be finished by manually rotating the objective lens. The principal difference from the conventional microscope or microscopic device is that: a counting chamber of the device is not arranged on an XY-direction motion mechanism but on a counting chamber sliding panel which is integrated with the platform into a whole; the XY-direction motion mechanism makes the counting chamber slide on the counting chamber sliding panel through an elastic connecting piece; and the horizontal height of the counting chamber is consistent in the moving process so as to ensure the definition of the acquired 40X pictures.
Description
Technical field
The invention belongs to the micro measurement technical field, be specifically related to the automatic testing method and the micro measurement device of arena.
Background technology
In the automatic urine sediments analyzer of existing employing microscopy mode, the method that the automatic detection of arena is adopted is as follows:
1, urine sample sucks counting chamber or tally and quiescent settling 30-70 about second.
2, completely adopt specialized range (general corresponding 0.3-1 microlitre left and right sides urine sample) arena 10X figure.
3, the whole 10X images of computer system analysis, analysis results such as the distribution of arena, quantity among the figure when obtaining.
4,, determine whether to move on to the relevant position and adopt figure with the 40X object lens according to 10X graphical analysis result.
5, analyze the automatic testing result that 40X figure obtains arena.
The micro measurement apparatus function of method is as follows above satisfying:
1, have automatic X, Y to the motion scan function, and running fix precision and flatness requirement height.
2, have automatic 10X, 40X tilting mirror function.
3,, need automatic adjusting of light intensity and Z to burnt function because of 10X, the required light intensity difference of 40X mirror and focal length variations.
By above-mentioned analysis as seen, the automatic testing method of the arena above adopting needs to adopt and has multi-functional and high-precision micro measurement device (low as if micro measurement device precision, can make testing result deviation big).In order to develop the automatic urine sediments analyzer of low cost, high reliability, improve detection method, the special-purpose micro measurement device that the reliable and satisfied detection of simplicity of design requires is just necessary.
Summary of the invention
The present invention proposes a kind of automatic testing method of arena, simplify requirement, and further propose a kind of simple and reliable micro measurement device that arena detects requirement automatically that satisfies the micro measurement apparatus function.
Technical scheme of the present invention is as follows:
A kind of automatic testing method of arena, described method is as follows:
(1) urine sample is sucked counting chamber or tally and quiescent settling, usually at 30-70 about second;
(2) completely adopt specialized range (general corresponding 0.3-1 microlitre left and right sides urine sample) arena 40X figure (this adopts the figure number is 16 times of 10X figure quantity in the existing method of tradition, and they can both represent the urine sample of certain volume);
(3) adopt the computer system whole 40X images of discriminance analysis that sieve, abandon blank or have only the image of impurity, only stay the image of sediment;
(4) analyze the 40X image that stays, obtain the automatic testing result of arena.Analytical approach is the same with prior art.
The present invention has also further provided the micro measurement device of realizing said method, and this device is made up of counting chamber horizontal scanning device, micro measurement device matrix, video camera, micro-40X object lens and micro-condenser system.
Wherein the counting chamber horizontal scanning device is made up of to motion, flexible connection piece, counting chamber sliding plate, counting chamber, counting chamber web joint to motion, Y to platform substrate, X XY.Y is installed in X on motion to motion, moves to motion with X.X is installed in XY on platform substrate to motion, is X to motion on platform substrate at XY.The counting chamber sliding plate is pasted to connect and is fixed on XY on platform substrate.The counting chamber web joint is placed on the counting chamber sliding plate, one side the linking together of the right and horizontally disposed flexible connection piece, the another side of flexible connection piece is connected Y on motion.Counting chamber is installed in the counting chamber web joint, and its bottom is attached on the counting chamber sliding plate.
Make XY when mobile when X drives flexible connection piece to motion and Y to motion, counting chamber web joint and counting chamber are made XY to slip on the counting chamber sliding plate under flexible connection piece drives;
XY is fixed in the micro measurement device matrix to platform substrate, above micro measurement device matrix video camera is installed, and over against the top position of counting chamber micro-40X object lens is installed in the centre, and micro-condenser system is installed below micro measurement device matrix.
From said apparatus as can be seen, it only adorns the 40X object lens, there is not Z to automatic focusing function, XY is fixed on one to motion platform substrate and microscope equipment matrix, platform rocks adopting the influence of figure when having reduced scanning and adopting figure, initial focusing can manually be rotated object lens and be finished, be not both with common microscope or microscope equipment maximum, the counting chamber of this device is not installed in XY on motion, but be placed on the smooth plates that combines as a whole with platform, XY slides counting chamber to motion by flexible connection piece on flat board, because flexible connection piece is very thin, easy elastic deformation up and down, horizontal distortion is little, when XY is doing XY scanning and adopts figure and move to motion, the deviation of its flatness just can not cause the variation of counting chamber focusing aspect, counting chamber remains and is fitted in that XY is to slip on the counting chamber sliding plate, and the level height in the counting chamber moving process is consistent, and has guaranteed the 40X picture clarity of gathering.
Adopt the figure effect for reaching good scanning, make things convenient for the automatic detection of arena, adopting figure with traditional microscope equipment needs its XY very high to the flatness of motion integral body.And adopt this microscope equipment, counting chamber remains and is fitted on the counting chamber sliding plate XY to slip, influence is adopted the factor of figure effect and has just been had only the flatness of counting chamber itself and counting chamber sliding plate, the flatness raising of single counting chamber sliding plate easily realizes manyly than XY to the flatness raising of motion integral body, and its XY can be not high to the flatness of motion.
As seen this method detects employed microscope equipment and has bigger simplification, has removed high low power and has changed mirror function, Z to automatic focusing function, and is small and exquisite simple.
Description of drawings
Fig. 1 is a kind of to satisfy the stereographic map of counting chamber horizontal scanning device that arena detects the micro measurement device of requirement automatically.
Fig. 2 is a kind of to satisfy the stereographic map that arena detects the micro measurement device of requirement automatically.
Embodiment
Describe the specific implementation that the inventive method is gathered arena 40X image in detail below in conjunction with the drawings and specific embodiments:
Realize method of the present invention wherein scanning collection 40X image can adopt micro measurement device illustrated in figures 1 and 2, this device includes counting chamber horizontal scanning device, micro measurement device matrix 8, video camera 9, micro-40X object lens 10 and micro-condenser system 11.
Its structure and assembly relation are as follows:
Referring to Fig. 1, the counting chamber horizontal scanning device is made up of to motion 3, flexible connection piece 4, counting chamber sliding plate 5, counting chamber 6, counting chamber web joint 7 to motion 2, Y to platform substrate 1, X XY.
Y is installed in X on motion 2 to motion 3, and X is installed in XY on platform substrate 1 to motion; Counting chamber sliding plate 5 is pasted to connect and is fixed on XY on platform substrate 1; Counting chamber web joint 7 is placed on the counting chamber sliding plate 5, and one end and flexible connection piece 4 are connected one, and flexible connection piece 4 is horizontally disposed with, and its another side is connected Y on motion 3; Counting chamber 7 is installed in the counting chamber web joint 7, and the bottom is attached on the counting chamber sliding plate 5; Make XY when mobile when XY drives flexible connection piece 4 to motion 2 and 3 like this, counting chamber web joint 7 and counting chamber 6 just can be made XY to slip on counting chamber sliding plate 5 under flexible connection piece 4 drives.
Referring to Fig. 2, XY is fixed in the micro measurement device matrix 8 to platform substrate 1, video camera 9 is installed above micro measurement device matrix 8, the micro-40X object lens 10 of middle installation, micro-condenser system 11 is installed in the below, so just formed a simple and practical sediment micro measurement device, initial focusing can manually be rotated object lens and be finished.
Utilize this device can realize that the 40X of high definition adopts collection and the analysis of figure, method is as follows:
1, urine sample is sucked counting chamber or tally and quiescent settling about 50 seconds;
2, with the mobile all around counting chamber 6 of counting chamber horizontal scanning device, completely adopt specialized range (corresponding 0.5 microlitre left and right sides urine sample) arena 40X figure, adopting in the figure process counting chamber 6 remains and is fitted in that XY has guaranteed to adopt the sharpness of figure to slip on the counting chamber sliding plate 5;
3, adopt the computer system whole 40X images of discriminance analysis that sieve, abandon blank or have only the image of impurity, only stay the image of sediment;
4, analyze the 40X image that stays, obtain the automatic testing result of arena.
Adopt above-mentioned counting chamber horizontal scanning device that counting chamber is moved and adopt figure, because flexible connection piece 4 is very thin, easy up and down elastic deformation, horizontal distortion is little, when XY is doing XY scanning and adopts figure and move to motion 2 and 3, the deviation of its flatness just can not cause the variation of counting chamber 7 focusing aspects, and counting chamber 6 can keep being fitted on the counting chamber sliding plate 5 XY all the time to slip; XY is fixed on micro measurement device matrix 8 to platform substrate 1, has reduced platform and has rocked, and these all make the image definition of camera acquisition be guaranteed.
Claims (2)
1. the automatic testing method of an arena is characterized in that by following sequence detection analysis:
(1) urine sample is sucked counting chamber or tally and quiescent settling;
(2) the arena 40X that completely adopts 0.3-1 microlitre urine sample schemes;
(3) adopt the computer system whole 40X images of discriminance analysis that sieve fast, abandon blank or have only the image of impurity, only stay the image of sediment;
(4) analyze the 40X image that stays, obtain the automatic testing result of arena.
2. micro measurement device that the arena of realizing above-mentioned detection method detects automatically, it is characterized in that: this device is made up of counting chamber horizontal scanning device, micro measurement device matrix (8), video camera (9), micro-40X object lens (10) and micro-condenser system (11);
Described counting chamber horizontal scanning device is made up of to motion (3), flexible connection piece (4), counting chamber sliding plate (5), counting chamber (6) and counting chamber web joint (7) to motion (2), Y to platform substrate (1), X XY;
Described Y is installed in X on motion (2) to motion (3), moves to motion (2) with X; Described X is installed in XY on platform substrate (1) to motion (2), is X to motion at XY on platform substrate (1);
Described counting chamber sliding plate (5) is pasted and fixed on XY on platform substrate (1); Counting chamber web joint (7) is placed on the counting chamber sliding plate (5), the right and horizontally disposed flexible connection piece (4) one side link together, the another side of flexible connection piece (4) is connected Y on motion (3);
Described counting chamber (6) is installed in the counting chamber web joint (7), and its bottom is attached on the counting chamber sliding plate (5);
Make XY when mobile when X drives flexible connection piece (4) to motion (2) and Y to motion (3), counting chamber web joint (7) and counting chamber (6) are made XY to slip on counting chamber sliding plate (5) under flexible connection piece (4) drive;
Described XY is fixed in the micro measurement device matrix (8) to platform substrate (1), in micro measurement device matrix (8) top video camera (9) is installed, over against the top position of counting chamber (6) micro-40X object lens (10) are installed in the centre, micro-condenser system (11) are installed in micro measurement device matrix (8) below.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102393705A (en) * | 2011-10-18 | 2012-03-28 | 长沙高新技术产业开发区爱威科技实业有限公司 | Sample formed component analyzer automatic detection control apparatus and control method thereof |
CN108760607A (en) * | 2018-07-22 | 2018-11-06 | 湖南乾康科技有限公司 | A kind of microscopic counting pond bracket plate positioning device |
CN111198262A (en) * | 2018-11-19 | 2020-05-26 | 苏州迈瑞科技有限公司 | Detection device and method for urine visible component analyzer |
CN111289511A (en) * | 2018-12-10 | 2020-06-16 | 苏州迈瑞科技有限公司 | Flatness adjusting method and system |
Citations (4)
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JPH10302067A (en) * | 1997-04-23 | 1998-11-13 | Hitachi Ltd | Pattern recognition device |
CN1292873A (en) * | 1998-03-07 | 2001-04-25 | 斯蒂芬·C·沃德罗 | Method and apparatus for performing chemical, qualitative, quantitative and semi-quantitative analyses of urine sample |
US20030133119A1 (en) * | 2002-01-17 | 2003-07-17 | Bachur Nicholas R. | Rapid imaging of particles in a large fluid volume through flow cell imaging |
CN1588063A (en) * | 2004-07-14 | 2005-03-02 | 沈一珊 | Full automatic urine sediment analytic system |
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2010
- 2010-07-02 CN CN2010102168051A patent/CN101893578B/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH10302067A (en) * | 1997-04-23 | 1998-11-13 | Hitachi Ltd | Pattern recognition device |
CN1292873A (en) * | 1998-03-07 | 2001-04-25 | 斯蒂芬·C·沃德罗 | Method and apparatus for performing chemical, qualitative, quantitative and semi-quantitative analyses of urine sample |
US20030133119A1 (en) * | 2002-01-17 | 2003-07-17 | Bachur Nicholas R. | Rapid imaging of particles in a large fluid volume through flow cell imaging |
CN1588063A (en) * | 2004-07-14 | 2005-03-02 | 沈一珊 | Full automatic urine sediment analytic system |
Non-Patent Citations (1)
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102393705A (en) * | 2011-10-18 | 2012-03-28 | 长沙高新技术产业开发区爱威科技实业有限公司 | Sample formed component analyzer automatic detection control apparatus and control method thereof |
CN108760607A (en) * | 2018-07-22 | 2018-11-06 | 湖南乾康科技有限公司 | A kind of microscopic counting pond bracket plate positioning device |
CN111198262A (en) * | 2018-11-19 | 2020-05-26 | 苏州迈瑞科技有限公司 | Detection device and method for urine visible component analyzer |
CN111198262B (en) * | 2018-11-19 | 2023-04-11 | 苏州迈瑞科技有限公司 | Detection device and method for urine visible component analyzer |
CN111289511A (en) * | 2018-12-10 | 2020-06-16 | 苏州迈瑞科技有限公司 | Flatness adjusting method and system |
CN111289511B (en) * | 2018-12-10 | 2024-04-09 | 深圳迈瑞生物医疗电子股份有限公司 | Flatness adjustment method and system |
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