CN101892244A - Oryza officinalis anti-Xanthomonas oryzae major gene Xa3/Xa26-2 and application for improving disease resistance of rice thereof - Google Patents

Abstract

The invention relates to the technical field of the plant genetic engineering, in particular to the isolation and cloning and functional verification of the DNA fragment of oryza officinalis anti-Xanthomonas oryzae major gene Xa3/Xa26-2. The Xa3/Xa26-2 gene is used to code the leucine-rich protein kinase protein. The Xa3/Xa26-2 gene ensures that rice can resist diseases caused by bacterial pathogen-Xanthomonas oryzae pv. oryzae. The DNA fragment and the regulatory sequence thereof are directly transferred in rice, and the resistance capability to Xanthomonas oryzae of the transgenic rice carrying Xa3/Xa26-2 is significantly enhanced.

Description

Oryza officinalis resisting bacterial leaf-blight major gene Xa3/Xa26-2 and its application in the improvement paddy disease-resistant

Technical field

The present invention relates to gene engineering technology field.Be specifically related to separating clone, functional verification and the application of a rice bacterial blight resistance major gene Xa3/Xa26-2.Described dna fragmentation can be given the paddy rice opposing by the microbial disease of bacterial leaf-blight.Directly change this fragment adjusting sequence endogenous with it over to plant materials, transgenic paddy rice can produce the defense response to bacterial leaf spot pathogenic bacteria by this gene mediated.

Technical background

Plant is subjected to the infringement of multiple pathogen in the process of growth.The phytopathy original of a great variety comprises virus, bacterium, fungi and nematode etc.The pathogen invaded plants causes two kinds of results: breeding in host plant of (1) pathogenic agent success causes relevant illness; (2) host plant produces disease resistance response, kills pathogen or stops its growth.Utilizing the disease resistance of resistant gene resource improvement plant, is the basic outlet that pre-disease prevention is protected environment simultaneously again.

The disease resistance response of plant is the complex process that polygene participates in regulation and control.The gene that participates in the plant disease-resistant reaction is divided into two classes: (1) disease-resistant (main effect) gene claims R (resistance) gene and (2) disease-resistant related gene again.

According to the understanding of present people's enantiopathy gene function, the product of disease-resistant gene mainly is as acceptor, and direct or indirect and cause of disease protein-interacting starts the intravital disease-resistant signal conducting path of plant (Chisholm etc., 2006).The disease resistance response strong resistance of disease-resistant gene mediation is good genetic resources.But the resource-constrained of disease-resistant gene in the arviculture kind.Excavating new disease-resistant gene from nearly edge species, to be used for the farm crop genetic improvement be the important goal that the investigator works always.

Paddy rice is an important crops in the world, but the influence of disease usually causes the decline of its yield and quality.Bacterial blight of rice causes by bacterial leaf spot pathogenic bacteria (Xanthomonas oryzae pv.oryzae), is in the world to one of the bacterial disease of rice hazard maximum (crossing Chong Jian, 1995).The resource of disease-resistant gene is very limited in the rice cropping kind (Oryza sativa L.).Have only about 30 (Chu Zhaohui and Wang Shi are flat, 2007) as the bacterial leaf spot resistant ospc gene of knowing at present.Because wild-rice is in wild state for a long time, has stood the natural selection of various poor environments and disaster, has formed extremely abundant genetic diversity.Containing in the wild-rice has good disease-resistant gene resource, is the valuable source storehouse that people excavate new excellent genes.Just there are a plurality of genes from wild-rice, to import to advance in the cultivated rice in known about 30 bacterial leaf spot resistant ospc genes.Be derived from the long medicine wild-rice in West Africa (Oryza longistaminata) as resisting bacterial leaf-blight dominant gene Xa21, high anti-to most of bacterial leaf-blight bacterial strain performances; But this gene is a strain-forming period resistance, promptly only becomes the strain phase just bacterial leaf spot pathogenic bacteria to be had complete resistance (Khush etc., 1990 in paddy rice; Wang etc., 1996).The separated clone of Xa21 gene (Song etc., 1995).One as yet not separated clone's resisting bacterial leaf-blight dominant gene Xa23 be derived from common wild-rice (Oryza rufipogon), it has resistance of wide spectrum (Zhang Qi etc., 2000).Another separated clone's bacterial leaf spot resistant ospc gene Xa27 come from tetraploid granule wild-rice (Orzyaminuta), these gene bacterial leaf spot resistant germ Philippines microspecies 2,3,5 and 6 (Amante-Bordeos etc., 1992; Gu etc., 2005).In addition, separated clone's bacterial leaf spot resistant ospc gene Xa29 (t) and Xa30 (t) are not (Tan Guangxuan etc., 2004 that identify from oryza officinalis (Orzya officinalis) and common wild-rice respectively as yet; Wang Chun company etc., 2004).

Summary of the invention

The objective of the invention is bacterial leaf spot resistant ospc gene Xa3/Xa26-2 that carries in the separating clone oryza officinalis (Oryza officinalis) and the dna fragmentation that comprises the promotor of regulating and control this gene, utilize this improvement of genes rice varieties or other plant to resist the ability of disease.

The present invention relates to separate and use the dna fragmentation that comprises the Xa3/Xa26-2 gene, its nucleotide sequence is shown in sequence table SEQ ID NO:1, and its encoding sequence is shown in 3284-6191 and 6295-6662 position among the sequence table SEQ ID NO:1.This gene (fragment) is given paddy rice to producing specific disease resistance response by the caused disease of bacterial leaf spot pathogenic bacteria (Xanthomonas oryzae pv.oryzae).This invention is applicable to all plants to this pathogenic bacteria sensitivity.These plants comprise monocotyledons and dicotyledons.Except that above-mentioned described dna fragmentation shown in SEQ ID NO:1, the defined gene of the present invention comprises also and is equivalent to the dna sequence dna shown in the SEQ IDNO:1 basically that perhaps its function is equivalent to the subfragment of sequence shown in the SEQID NO:1.Sequence shown in the sequence table SEQ ID NO:1 is carried out bioinformatic analysis to be shown, a kind of receptor protein kinase of this dna sequence encoding, it comprises extracellular rich leucine and repeats (leucine-rich repeat, LRR) structural domain and intracellular protein kinase structural domain.

Can adopt the Xa3/Xa26-2 gene of having cloned to make probe, from cDNA and genomic library, screen gene of the present invention or homologous gene.Equally, adopt PCR (polymerase chain reaction) technology, also can from genome, mRNA and cDNA, increase obtain Xa3/Xa26-2 gene of the present invention and any interested section of DNA or with its homologous section of DNA.Adopt above technology, can separate the sequence that obtains comprising the sequence of Xa3/Xa26-2 gene or comprise one section Xa3/Xa26-2 gene, this sequence is connected with suitable carriers, can change vegetable cell over to, and express the Xa3/Xa26-2 gene, produce disease-resistant transgenic plant.Adopting this transgenic technology to create disease-resistant plants is that traditional breeding technology institute is inaccessiable.

Change clone's disease-resistant gene over to susceptible plant, help to produce new disease-resistant plants.Particularly can be with genetic transformation technology a plurality of disease-resistant genes that in plant, add up, and can not produce do not follow in the traditional breeding technology appearance with the burdensome kind that needs improvement of introducing of genetic linkage.Simultaneously, the clone of disease-resistant gene can overcome traditional breeding method can not shift disease-resistant gene between plant species problem.Disease-resistant transfer-gen plant that the present invention can further provide or the above-mentioned dna fragmentation of applications exploiting obtains and corresponding seed can change gene of the present invention over to other plant with the mode of sexual hybridization.

The present invention provides a kind of new method for strengthening paddy rice to the resistance of bacterial leaf-blight.This method comprises that Xa3/Xa26-2 gene and its regulating DNA sequence that will derive from wild-rice are connected, change over to susceptible paddy rice with the genetic transformation carrier, and the improvement paddy rice is to the resistance of bacterial leaf-blight.

More detailed technical scheme sees that " embodiment " is described.

Description of drawings

Sequence table SEQ ID NO:1. is the dna sequence dna of the present invention's Xa3/Xa26-2 gene of cloning.

Fig. 1. the schema of evaluation of the present invention and separating clone rice bacterial blight resistance gene Xa3/Xa26-2 and checking Xa3/Xa26-2 gene function.

Fig. 2. the structure of Xa3/Xa26 gene and MRKa gene and PCR primer (arrow) position that is used to prepare dna probe in the rice variety bright extensive 63.

The dna probe of Fig. 3 .Xa3/Xa26 gene fragment and 9 BAC clones' results of hybridization.λ-ECoT14I is dna molecular size criteria marker (DNAladder; Available from Dalian TaKaRa company).

Fig. 4. Xa3/Xa26 gene family member's distribution and sequence homology in rice variety bright extensive 63 and the oryza officinalis.Each arrow is represented an Xa3/Xa26 family member and transcriptional orientation.Xa3/Xa26 and Xa3/Xa26-2 gene downstream have 1.4kb nucleotide sequence homology degree to reach 83% approximately.11.9kb section is to be used for the genetic transformation dna fragmentation.

Fig. 5. the structure of genetic transformation carrier pCAMBIA1301.

Fig. 6 .T ₁For the reporter gene GUS in the genetic transformation plant and disease-resistant phenotype be divided into from.Illustrate with the closely linked Xa3/Xa26-2 gene of gus gene and disease-resistant phenotype be divided into from.Contrast is paddy rice susceptible variety Mudanjiang 8 (acceptor of genetic transformation).Lesion area is the data of inoculation bacterial leaf spot pathogenic bacteria PXO61 after two weeks.

Fig. 7. compare with contrast Mudanjiang 8, the genetic transformation plant (D101OM43) of carrying the Xa3/Xa26-2 gene has all strengthened the resistance of different bacterial leaf spot pathogenic bacterias.

Fig. 8 .Xa3/Xa26-2 gene structure.Arrow shows PCR primer location and the direction that is used for the gene structure analysis." ATG " and " TGA " is respectively translation initiation password and termination codon.Numeral is shown the Nucleotide number of each structure.

Embodiment

Early-stage Study result of the present invention shows that the resisting bacterial leaf-blight dominant gene Xa3/Xa26 that derives from long-grained nonglutinous rice (Oryza sativa L.ssp.indica) kind bright extensive 63 belongs to the multigene family member; The member of this family is positioned at paddy rice No. 11 chromosomal long-armed (Yang etc., 2003 with the form that is arranged in series; Sun etc., 2004,2006; Xiang etc. 2006).In bright extensive 63, Xa3/Xa26 family is made up of 4 members (Xa3/Xa26, MRKa, MRKc and MRKd), and the dna sequence dna homology between their coding region is 60% to 78% (Sun etc., 2004).Rich leucine of Xa3/Xa26 coding repeats (leucine-richrepeat, LRR) protein kinase proteinoid (Sun etc., 2004).Target gene in according to the present invention and Xa3/Xa26 gene order homology analysis and it in the Xa3/Xa26 family of oryza officinalis (Oryza officinalis) with other members' opposite position relation, determine that this gene is the allelotrope of Xa3/Xa26 gene, so called after Xa3/Xa26-2 gene (looking the Xa3/Xa26 gene is Xa3/Xa26-1).

Further definition the present invention in following examples.Fig. 1 has described the flow process of evaluation and separating clone Xa3/Xa26-2 gene and checking Xa3/Xa26-2 gene function.According to following description and these embodiment, those skilled in the art can determine essential characteristic of the present invention and under the situation that does not depart from spirit and scope of the invention, can make various changes and modification to the present invention, so that its suitable various uses and condition.

Embodiment 1: separating clone Xa3/Xa26-2 gene and gene structure analysis from oryza officinalis

1. identify and carry the large fragment DNA of Xa3/Xa26 dna homolog sequence

Researchist of the present invention at first uses the specific PCR primer RKa-11L (5 '-TTGGCTTGAACGGCTTAACT-3 ') of the specific PCR primer RKb-3 ' race2 (5 '-TGGTCAAATACCGGAAGGAG-3 ') of Xa3/Xa26 gene and RKb-2R (5 '-CAGTCCACCACATGGACAAG-3 ') and Xa3/Xa26 family member MRKa gene and RKa-1R (5 '-AAGATGAAATATGCTCGGTGGT-3 ') to amplify the dna fragmentation of Xa3/Xa26 gene and MRKa, each about 1kb (Fig. 2) of amplification length from bright extensive 63.These two sections pcr amplification products are mixed genome BAC (bacterialartificial chromosome) library (Ammiraju etc., 2006) of screening oryza officinalis (Oryza officinalis) as probe, identified 9 BAC clone.These 9 BAC clone (Ammiraju etc., 2006) are so kind as to give by Rod professor Wing of U.S. University of Arizona university.To screen to such an extent that BAC clones enlarged culturing, adopt standard base (SB) cracking process separation and Extraction BAC plasmid (Sambrook and Russell, 2001).With gained BAC plasmid with Hind III complete degestion, electrophoresis, be transferred to nylon membrane; Hybridize (Sambrook and Russell, 2001) with the nylon membrane that is loaded with the BAC plasmid respectively with above-mentioned Xa3/Xa26 gene fragment probe and MRKa gene fragment probe then.The results of hybridization of making probe with the Xa3/Xa26 gene fragment shows that BAC clone OOBa0120J21 has maximum hybrid belts (Fig. 3), infers that this BAC clone may cover Xa3/Xa26 gene family section.Decision is checked order to the OOBa0120J21 clone.

2.BAC the structure in clone's shotgun library

Researchist of the present invention has at first made up the OOBa0120J21 clone's who is used to check order shotgun library.Adopt supersonic method to make up shotgun library (Sambrook and Russell, 2001).Circular plasmids 1.6 seconds (Ultrasonic Cell Disruptor Soniprep 150 is a SANYO company product, and concrete operations are with reference to the working instructions of this instrument) with ultrasonication OOBa0120J21 clone.Separate the dna fragmentation of about 2.0-3.5kb by 1% agarose gel electrophoresis, reclaim test kit (giving birth to worker's biotechnology company limited) purify DNA from running gel available from Chinese Shanghai with UNIQ-10 pillar DNA glue.DNA T behind the purifying ₄Archaeal dna polymerase is mended flat terminal, be connected with dephosphorylized pUC19 carrier (available from the U.S. Amersham Bioscience company) flush end that the Restriction enzyme Sma I enzyme is cut, (electric conversion instrument is an eppendorf company product to electricity transformed into escherichia coli DH10B (available from American I nvitrogen company), the present embodiment applied voltage is 1800V, and concrete operations are with reference to the working instructions of this instrument).Choose the clone and detect the insertion clip size: extract the positive colony plasmid, and carry out electrophoresis with empty pUC19 plasmid and compare, reject false positive clone and cloning of small fragment, or with EcoRI and HindIII double digestion recombinant plasmid, detect and insert clip size, screening is inserted the suitable clone of clip size and is used for order-checking as the shotgun library.

3.Shotgun the order-checking in library

Adopt M13-F (5 '-GTAAAACGACGGCCAGT-3 ') and M13-R 5 '-CAGGAAACAGCTATGAC-3 ') sequencing kit (BigDye Kit) of universal primer (giving birth to worker's biotechnology company limited), U.S. Perkin Elmer company available from Chinese Shanghai, according to the test kit specification sheets with the terminal cessation method of dideoxy nucleotide respectively from the shotgun library clone two ends of random choose check order.

4. the splicing of original series

Use Squencer 4.5 softwares (U.S. Gene Codes Corporation) splicing sequence.Automatically remove relatively poor sequence of end sequencing and pUC19 carrier sequence with Squencer4.5 software; Software is not removed the then deletion by hand of clean sequence.The DNA of bacteria sequence of the chip sequence of BAC carrier and pollution is removed by the method (Altschul etc., 1997) that compares with the BAC sequence or BLAST analyzes.With Squencer 4.5 softwares to the parameter that two sequences splices be: overlap length (Mini Overlap) is greater than 20bp (base pair), and the consistence of overlap (Mini Match) is greater than 85%.Each base is established a capital really will be with reference to a plurality of shotgun fragment sequences that overlap this site.For the zone that is covered by shotgun fragment sequence sequence verification once more, to guarantee the accuracy of base.

5.DNA sequential analysis

At first use Blastn and Blastx method (Altschul etc., 1997) to analyze institute's calling sequence.Adopt Fgenesh (http://www.softberry.ru/berry.phtml) (Salamov and Solovyev then, 2000) and GeneMark.hmm (http://exon.gatech.edu/GeneMark/eukhmm.cgi) (Besemer and Borodovsky, 2005) software analysis predicted gene structure, analytical results further uses Blastp method (Altschul etc., 1997) to determine.Article two, or the comparative analysis of many Nucleotide or aminoacid sequence use BLAST2 sequence method (Tatusova etc., 1999) and ClustalW method (http://www.ebi.ac.uk).

BAC clone OOBa0120J21 order-checking and sequence assembly are formed three big sequence fragments, and its length is respectively 84.1kb, 25.8kb and 3.3kb.Sequential analysis finds to have 4 genes encoding LRR-protein kinase proteinoids on the fragment of 84.1kb, with the product of rice bacterial blight resistance gene Xa3/Xa26 coding in various degree homology is arranged, they are named as OoRKa, OoRKb1, OoRKb and OoRKf (Fig. 4).With Fgenesh, GeneMark.hmm and Blastx analysis revealed, OoRKa, OoRKb and OoRKf are 3 complete genes, and OoRKb1 is an imperfect gene (Fig. 4).The transcriptional orientation of OoRKa and OoRKb is identical, and the transcriptional orientation of OoRKf opposite with OoRKa and OoRKb (Fig. 4).

Xa3/Xa26 gene family section in this section and the paddy rice bright extensive 63 is compared analysis.The homology degree of finding the MRKa gene in the OORKa gene and bright extensive 63 in the oryza officinalis reaches 80%, the OoRKb gene in the oryza officinalis and and bright extensive 63 in Xa3/Xa26 (being called MRKb again) dna homolog degree up to 95%; And OoRKb and MRKb gene downstream have 1.4kb section homology degree to reach 83% approximately, so OoRKb is the allelotrope of Xa3/Xa26, we name this gene is Xa3/Xa26-2 (Fig. 4).

The functional verification of embodiment 2:Xa3/Xa26-2 gene

1. the structure of genetic transformation carrier

Used carrier of the present invention is pCAMBIA1301 (Fig. 5), and it is the rice genetic conversion carrier of using always (Sun etc., 2004).Go up enzyme with restriction enzyme EcoRV from BAC clone OOBa0120J21 and cut the fragment (Fig. 4) that back recovery 11.9kb comprises coding region, promotor and the afterbody sequence of Xa3/Xa26-2 gene.Simultaneously, cut genetic transformation carrier pCAMBIA1301 with the Restriction enzyme Sma I enzyme; Enzyme cuts complete, with SAP (shrimp alkaline phosphotase) dephosphorylation; Use chloroform: primary isoamyl alcohol (volume ratio 24: 1) extracting, purifying enzyme are cut product.Cut back to close fragment and the good carrier of purifying is done ligation with the enzyme that comprises the Xa3/Xa26-2 gene.Cut the checking positive colony by enzyme, the recombinant plasmid of acquisition is named as D101O.

2. genetic transformation and T ₀Analyze for the genetic transformation plant

Adopt agriculture bacillus mediated genetic transforming method (Lin and Zhang, 2005) that D101O is imported paddy rice susceptible variety Mudanjiang 8 (Oryza sativa ssp.japonica).The genetic transformation plant that obtains is named as D101OM (previous section of this name is that D101O is the genetic transformation container name, and M represents rice varieties Mudanjiang 8).The present invention obtains independent transformed plant 33 strains altogether.To half transformed plant inoculation bacterial leaf-blight bacterial strain PXO61 wherein, second half transformed plant inoculation bacterial leaf-blight bacterial strain PXO341.The bacterial leaf spot pathogenic bacteria inoculation adopts leaf-cutting method that the paddy rice that becomes the strain phase is inoculated (Sun etc., 2004).Bacterial leaf-blight bacterial strain PXO61 and PXO341 are so kind as to give (Sun etc., 2004 by the Philippines International Rice Research Institute; Wu etc., 2008).The method of having published (Sun etc., 2004) is followed in the cultivation of bacterial leaf spot pathogenic bacteria.Inoculate 14 days " Invest, Then Investigate " lesion areas (scab length/sick leaf length * %).Compare with contrast Mudanjiang 8 and the negative plant of genetic transformation, the resistance of the positive genetic transformation plant of the overwhelming majority significantly strengthens (table 1).

Table 1.T ₀Phenotype for genetic transformation plant (D101OM) inoculation bacterial leaf-blight bacterial strain PXO61 and PXO341

⁽¹⁾Every strain genetic transformation gene plant inoculation 3-5 sheet leaf, 14 days " Invest, Then Investigate " scabs and sick leaf length, each data is from the mean+SD of a plurality of blades.

⁽²⁾Negative transformed plant, the positive transformed plant of other plant.

3. the coseparation analysis of genetic transformation plant

For the resistance against diseases of verifying the genetic transformation plant whether strengthen with the Xa3/Xa26-2 gene change over to relevant, at T ₀T for resistance enhanced 3 strain genetic transformation plant (D101OM1, D101OM14, D101OM43) ₁Carry out genotype and resistance coseparation analysis for family.Inoculation bacterial leaf spot pathogenic bacteria PXO61 inoculates 14 days " Invest, Then Investigate " lesion areas when boot stage.The DNA extraction of taking a sample simultaneously, the PCR primer Gus2F (5 '-CCAGGCAGTTTTAACGATCAGTTCGC-3 ') and Gus2R (5 '-GAGTGAAGATCCCTTTCTT GTTACCG-3 ') the augmentation detection positive genetic transformation plant of adopting the entrained reporter gene GUS (GRD beta-glucuronidase) of genetic transformation carrier.Analyzing resistance enhancing and the gus gene of finding the genetic transformation plant is divided into from (Fig. 6).The resistance enhancing that the genetic transformation plant is described is the existence because of the Xa3/Xa26-2 gene, and Xa3/Xa26-2 is a bacterial leaf spot resistant ospc gene.

4. the anti-spectrum analysis of genetic transformation plant

To T ₁Inoculate different bacterial leaf-blight bacterial strains in boot stage for genetic transformation family D101OM43 and contrast Mudanjiang 8, analyze the anti-spectrum of genetic transformation plant.Above-mentioned leaf-cutting method is adopted in inoculation.Philippines bacterial leaf-blight bacterial strain PXO61, PXO71, PXO112, PXO341 are so kind as to give (Sun etc., 2004 by the Philippines International Rice Research Institute; Wu etc., 2008).Chinese white leaf blight bacterial strain is bacterial strain (Sun etc., 2004 commonly used; Li etc., 2009).Japanese strain T7174 also is a bacterial leaf-blight bacterial strain (Cao etc., 2007) commonly used.Compare with genetic transformation acceptor rice varieties Mudanjiang 8, the genetic transformation plant of carrying the Xa3/Xa26-2 gene has significantly strengthened (P＜0.01) to the resistance (Fig. 7) of different bacterial leaf-blight bacterial strains.The lesion area of genetic transformation plant just contrasts 12% to 75% of Mudanjiang 8.These presentation of results Xa3/Xa26-2 gene pairs bacterial leaf spot pathogenic bacteria has resistance of wide spectrum.

5.Xa3/Xa26-2 gene structure and coded product analysis

Researchist of the present invention utilizes transfer-gen plant (D101OM) to analyze the structure of Xa3/Xa26-2 gene.TRIzol Reagent (American I nvitrogen company) is adopted in the extracting of total RNA of transfer-gen plant, and working method is undertaken by the specification sheets that company provides.

To be used for DNA enzyme I (the American I nvitrogen company) processing of total RNA of reverse transcription with no RNA enzymic activity earlier, room temperature was placed 15 minutes, removed the DNA that may exist among total RNA and polluted; Again total RNA was handled deactivation DNA enzyme I 10 minutes at 65 ℃.With SuperScript III ThermoScript II (American I nvitrogen company), carry out reverse transcription by its specification sheets that provides.Adopt PCR primer RKb3F and RKb2R (table 2) amplifying genom DNA and the RNA reverse transcription product of crossing over the prediction introne position, the RT-PCR product is littler than genome amplification product, illustrates that there is an intron (Fig. 8) really in this section.Adopt other primer (table 2) of Xa3/Xa26-2 constant gene segment C to carry out reverse transcription (RT)-pcr analysis, do not find the existence of other intron again.

The gene terminal sequence analysis adopts the Clontech SMARTer RACE cDNA Amplification Kit of company test kit, according to the test kit specification sheets, by RACE (rapid amplification of cDNA end) analytical procedure, determine 5 ' and 3 ' end sequence of Xa3/Xa26-2 gene.

Table 2. is used for the gene-specific primer of RT-PCR and RACE analysis

Adopt above-mentioned dna sequencing method, the RT-PCR product is checked order.The cDNA sequence and the genome sequence of comparative analysis Xa3/Xa26-2 gene determine that this gene is made up of 3600 Nucleotide, comprise two exons and an intron (Fig. 8).First exon is formed (the 3238-6191bp place that is positioned at sequence table SEQ ID NO:1) by 2954 Nucleotide, (untranslated region UTR) (is positioned at the 3238-3283bp place of sequence table SEQ ID NO:1) and the coding regions (being positioned at the 3284-6191bp place of sequence table SEQ ID NO:1) of 2908 Nucleotide compositions wherein to comprise 5 ' the end non-translational region be made up of 46 Nucleotide; Intron is formed (the 6192-6294bp place that is positioned at sequence table SEQ ID NO:1) by 103 Nucleotide; Second exon formed (the 6295-6837bp place that is positioned at sequence table SEQ ID NO:1) by 543 Nucleotide, wherein comprises coding region of being made up of 368 Nucleotide (being positioned at the 6295-6662bp place of sequence table SEQ ID NO:1) and 3 ' the end UTR (being positioned at the 6663-6837bp place of sequence table SEQ ID NO:1) that is made up of 175 Nucleotide.

LRR receptor kinase proteinoid of forming by 1092 amino acid of Xa3/Xa26-2 genes encoding.The proteic amino acid consistence of XA3/XA26-2 albumen and XA3/XA26 is 92%, and amino acid whose homology is 95%.

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Sun?X，Cao?Y，Wang?S(2006)Point?mutations?with?positive?selection?were?a?major?force?during?theevolution?ofa?receptor-kinase?resistance?gene?family?of?rice.Plant?Physiol.140：998-1008.

Tatusova?TA，Madden?TL(1999)BLAST2?Sequences，a?new?tool?for?comparing?protein?andnucleotide?sequences.FEMS?Microbiol.Lett.174：247-250.

Wang?GL，Song?WY，Ruan?DL，Sideris?S，Ronald?PC(1996)The?cloned?gene，Xa21，confersresistance?to?multiple?Xanthomonas?oryzae?pv.oryzae?isolates?in?transgenic?plants.Mol.Plant-Microbe?Interact.9：850-855.

Wu?X，Li?X，Xu?C，Wang?S(2008)Fine?genetic?mapping?of?xa24，a?recessive?gene?for?resistanceagainst?Xanthomonas?oryzae?pv.oryzae?in?rice.Theor.Appl.Genet.118：185-191.

Xiang?Y，Cao?Y，Xu?C，Li?X，Wang?S(2006)Xa3，conferring?resistance?for?rice?bacterial?blight?andencoding?a?receptor?kinase-like?protein，is?the?same?as?Xa26.Theor.Appl.Genet.113：1347-1355.

Yang?Z，Sun?X，Wang?S，Zhang?Q(2003)Genetic?and?physical?mapping?of?a?new?gene?for?bacterialblight?resistance?in?rice.Theor.Appl.Genet.106：1467-1472.

Sequence table

＜110〉Hua Zhong Agriculture University

＜120〉oryza officinalis resisting bacterial leaf-blight major gene Xa3/Xa26-2 and its application in the improvement paddy disease-resistant

<130>

<141>2010-03-17

<160>2

<170>PatentIn?version?3.3

<210>1

<211>11848

<212>DNA

<213>Oryza?sativa

<220>

<221>gene

<222>(3238)..(6837)

<220>

<221>5’UTR

<222>(3238)..(3283)

<220>

<221>CDS

<222>(3284)..(6191)

<220>

<221>Intron

<222>(6192)..(6294)

<220>

<221>CDS

<222>(6295)..(6662)

<220>

<221>3’UTR

<222>(6663)..(6837)

<400>1

tatcttagat?atgatgagca?tgtgcaccat?tagacgtata?ataaagataa?agggcatttt 60

ataatataga?ttatatcatt?ctatatcatt?ttataaacat?acatagccaa?agtcatgtgt 120

gatttacttt?aaatctaact?aaaaaaatca?tgtctacaat?aataagaatc?atagaaattt 180

aaaaaacaga?aaaactcaag?agcatatacc?ctaatagtaa?ataaaaatat?caatggctaa 240

tccctaggac?atataaaatt?ataaaggttt?gttaaacgtc?ctatacaacc?ctcctacagt 300

gtctattaaa?gtctttaaca?aataagagaa?aactttaaaa?ttcttctata?atagacaaag 360

catccaacac?ttaatcctca?ttaatttaat?gctcccatta?attttagcca?ttggtcatgt 420

caatgggtat?gtgtatacac?acattattta?ataaaaaaac?catccattca?agcgtgtttt 480

gtaatagaaa?attatttgag?atgtcaggtg?aggttgtagt?gacctgttgc?aaggttcaca 540

gggttgtagc?aagaatgacc?agcatcaagt?tgcaagaggt?tgccagccat?ctaatgtatc 600

actaaaagag?cccataggag?atatttgtgt?tttgttgatt?agagccacaa?ccaaaagaag 660

gcaacaagag?aaccccaaca?caaataattt?ttatgaataa?accttctctc?aactcaagta 720

tctatgtaat?attatttaag?tcgcatcagc?ttgaaacatg?cataatttga?taacatggta 780

tatctagaag?gctatatgtt?agaacattca?attaacccac?cttagaaata?ataatactta 840

gcaagaactc?atgcctgtaa?caaatttcta?tctttaattt?tgatatttaa?ttttatcact 900

gaaaaggggg?agatagagta?tgtgaatatt?gtccaatttg?caagctaaat?gtttttttaa 960

atgtgatagt?tcagttggtt?actgattctc?aagtattatc?atactattca?agagtagagc?1020

aagcaataat?catatccaaa?acagatacaa?agaacttaca?tatcatagat?aattttaaca?1080

tttagaaaca?cgaagatgcc?tgcgcaaccg?cgtgggctac?cttcctagtt?aatataaata?1140

tgtaaaggta?taacttaaga?ttataattta?tttgatgata?aaacaagtat?ttatgtatat 1200

atatatatat?atatttaata?aaatgaatga?tcaaacgcta?tacgaaaaat?caacggcgtc 1260

gtgcattttg?aaatggagga?agtatttttt?ttcgaggtaa?tcgttatgtt?tgaggaaatg 1320

tggcatgagt?tcaccacttg?ccaacttgga?atataacaca?tcgtatcgtt?agtgttccat 1380

gcactaaaag?tctctttctt?tcccctttgg?aatgagaaca?atatagcttg?gtaatactgt 1440

tcatcttatt?cgcctttttc?ctcctttgga?aagggacagt?tgagcggtgg?tctggtggtt 1500

acatctaaaa?catgccatga?gaagctttgg?cactgtgtgt?cttgtgcttc?aaatggctcg 1560

acattacaac?ctaataaatc?tgaacacttt?gttccatctt?ggacttcaga?gcactggtac 1620

ttcagtacat?gcccaagggt?agcttagaag?cactctgcac?tcagaacaag?gaaagcaatt 1680

aggctttctc?cagaggttgg?ttattatgct?agatgtgtca?atggcaatgg?aatacctaca 1740

tcatgagcac?tatgaggtgg?tcttacacta?cgatctgaag?cctagcaacg?tactatttga 1800

cgatgatatg?atggcacatg?tgggagactt?tggcattgca?aggttattgt?taggtgatga 1860

caactccatg?atctcagcta?gcatgccagg?aacagttggg?tacatggcac?caggtactta 1920

gtactagttt?ttgttgtctt?gctcaaacat?tgcctgatat?tttattatta?tcaagtaggg 1980

tgcaactaat?ttttggttgt?ctatctttct?taagcagagt?atggggctct?tggaaaagca 2040

tcacagaaga?gcgacgtgtt?cagctacggg?atcatgctac?tcgaagtgtt?cactaggaag 2100

agatccacag?atgttatgtt?tgtaggagaa?ctgaacatca?ggcagtgggt?tcaccaggcg 2160

tttcctgcag?agcttgtcca?tttgatggac?tgccaacttc?tacaggatgg?ctcttcttct 2220

tcttccagta?acatgcatgg?cttccttgtg?ccagtgttcg?agctgggctt?gctctgctca 2280

gctgactccc?cggagcaaag?gatggcgatg?agcgatgtgg?tcgtgacact?gaagaagatt 2340

aggaaggact?atgtcaaatt?gatggcaacc?acagggagcg?ctgtgcagca?atgatccatc 2400

gctctctcgt?ggtatatgag?cgaataaaat?atatatcctt?tgcatccatt?tcttcttctg 2460

catcaggaat?agcatcagtg?catgcccagt?gatcgattac?cctatttgtg?tacggttgaa 2520

ttgaatatat?ctgtggtgct?tcaggttcag?caataattta?gttggtgtaa?aaatgtgatt 2580

gaactgttgg?tcaataaatt?tgcatgatga?aaatgggagt?agatgatgtg?ctgcttatgt 2640

tttcttattt?ctggccaaaa?taaataaaaa?aaggaatatt?ctgggcacag?catcacaact 2700

ccggctcaat?cagccttaaa?cagccacagt?taacagtcct?aagcagagaa?acttaacaag 2760

cttttcaggc?aaacaaaaca?tcaaaaggtc?cacaagacaa?cagggtcttc?agggagcaca 2820

tcttcaggct?gtgatgcaaa?aaggatctga?cagccgtatg?ataactactg?aacaggtcgt 2880

gtatattgat?aaggtccgcc?actaaccacc?caatcaacaa?aagagtaagg?ccgttgccgt 2940

caaattgttt?gacagaaaaa?aaaaatttca?gtgagtgtat?ggctgatgac?cgagcgacac 3000

agctatcata?tctagcgtgt?cgtgcacacc?gcgatctgct?gaatatatat?tttgtgatga 3060

ctttattttc?cagcgtttac?ttagtagtgc?tgccaaatat?ttatgactgg?aatttgactg 3120

gagggagtat?catttaagtt?tctttcactt?tctgagagca?acagtcaagg?tcgtccgaga 3180

tgttgaaagc?aagctagcac?tactgtgcta?aataaagctc?aacttgatcg?tcactgtgaa 3240

gtatgatgca?ctcttgttgc?caatgcatca?cacacaacca?gac?atg?gcg?ctt?gga 3295

Met?Ala?Leu?Gly

1

ttg?cca?gta?tgg?att?ttc?gtt?gcg?ttg?ttg?atc?gct?ttg?tcc?act?gtg 3343

Leu?Pro?Val?Trp?Ile?Phe?Val?Ala?Leu?Leu?Ile?Ala?Leu?Ser?Thr?Val

5 10 15 20

cct?tgt?gct?tcc?tct?cta?ggt?ccg?agc?aag?agt?aac?ggc?agt?gac?atc 3391

Pro?Cys?Ala?Ser?Ser?Leu?Gly?Pro?Ser?Lys?Ser?Asn?Gly?Ser?Asp?Ile

25 30 35

gac?ctc?gct?gca?ctt?ttg?gct?tta?aaa?tcg?cag?ttc?tct?gat?cct?gat 3439

Asp?Leu?Ala?Ala?Leu?Leu?Ala?Leu?Lys?Ser?Gln?Phe?Ser?Asp?Pro?Asp

40 45 50

aac?att?ctt?gcc?ggc?aac?tgg?acc?att?ggc?acg?cca?ttc?tgc?caa?tgg 3487

Asn?Ile?Leu?Ala?Gly?Asn?Trp?Thr?Ile?Gly?Thr?Pro?Phe?Cys?Gln?Trp

55 60 65

atg?ggt?gtc?tcg?tgc?agc?cac?cgc?cgg?cag?cgc?gtc?acc?gcc?ctg?aaa 3535

Met?Gly?Val?Ser?Cys?Ser?His?Arg?Arg?Gln?Arg?Val?Thr?Ala?Leu?Lys

70 75 80

ctg?cca?aac?gtt?cct?ctc?caa?gga?gag?ctc?agc?tct?cac?ctt?ggt?aac 3583

Leu?Pro?Asn?Val?Pro?Leu?Gln?Gly?Glu?Leu?Ser?Ser?His?Leu?Gly?Asn

85 90 95 100

att?tct?ttt?ctc?ttg?atc?ctc?aac?ctc?acc?aac?acc?ggc?ctc?aca?ggc 3631

Ile?Ser?Phe?Leu?Leu?Ile?Leu?Asn?Leu?Thr?Asn?Thr?Gly?Leu?Thr?Gly

105 110 115

ttg?gtg?ccg?gat?tat?ata?gga?agg?cta?cgt?cgc?ctt?gag?atc?ctt?gat 3679

Leu?Val?Pro?Asp?Tyr?Ile?Gly?Arg?Leu?Arg?Arg?Leu?Glu?Ile?Leu?Asp

120 125 130

ctc?ggc?cac?aat?gcc?ttg?tca?ggt?ggc?gtc?cca?atc?gcc?ata?ggg?aac 3727

Leu?Gly?His?Asn?Ala?Leu?Ser?Gly?Gly?Val?Pro?Ile?Ala?Ile?Gly?Asn

135 140 145

ctc?acg?agg?ctt?cag?cta?ctt?aat?cta?cag?ttt?aac?cag?cta?tat?ggt 3775

Leu?Thr?Arg?Leu?Gln?Leu?Leu?Asn?Leu?Gln?Phe?Asn?Gln?Leu?Tyr?Gly

150 155 160

cca?atc?cca?gca?gag?ctg?cag?ggg?ctg?cac?agt?ctt?gac?agc?atg?aat 3823

Pro?Ile?Pro?Ala?Glu?Leu?Gln?Gly?Leu?His?Ser?Leu?Asp?Ser?Met?Asn

165 170 175 180

ctc?cgt?cac?aat?tac?ctc?act?gga?tca?att?ccg?gac?aat?ctg?ttc?aac 3871

Leu?Arg?His?Asn?Tyr?Leu?Thr?Gly?Ser?Ile?Pro?Asp?Asn?Leu?Phe?Asn

185 190 195

aac?aca?tct?ttg?cta?act?tat?ctc?aac?gtt?ggt?aac?aat?agc?ctg?tca 3919

Asn?Thr?Ser?Leu?Leu?Thr?Tyr?Leu?Asn?Val?Gly?Asn?Asn?Ser?Leu?Ser

200 205 210

gga?ccg?ata?ccg?ggt?tgc?atc?ggt?tcc?ttg?cca?atc?ctc?caa?tac?ctt 3967

Gly?Pro?Ile?Pro?Gly?CysIle?Gly?Ser?Leu?Pro?Ile?Leu?Gln?Tyr?Leu

215 220 225

aac?ttg?cag?gcc?aat?aac?tta?act?ggg?gcg?gtg?cca?cca?gcc?atc?ttc 4015

Asn?Leu?Gln?Ala?Asn?Asn?Leu?Thr?Gly?Ala?Val?Pro?Pro?Ala?Ile?Phe

230 235 240

aac?atg?tct?aaa?tta?agt?act?att?tct?ctt?ata?tcg?aat?ggt?tta?act 4063

Asn?Met?Ser?Lys?Leu?Ser?Thr?Ile?Ser?Leu?Ile?Ser?Asn?Gly?Leu?Thr

245 250 255 260

ggc?cct?atc?cct?ggt?aat?aca?agt?ttc?agc?ctc?cca?gtt?cta?caa?tgg 4111

Gly?Pro?Ile?Pro?Gly?Asn?Thr?Ser?Phe?Ser?Leu?Pro?Val?Leu?Gln?Trp

265 270 275

ttc?gcc?atc?agt?aaa?aac?aat?ttc?ttt?ggt?caa?att?cca?ctg?ggg?ctc 4159

Phe?Ala?Ile?Ser?Lys?Asn?Asn?Phe?Phe?Gly?Gln?Ile?Pro?Leu?Gly?Leu

280285290

gca?gcg?tgt?cca?tac?ctc?caa?gtt?att?gcc?ctg?cct?tat?aat?tta?ttc 4207

Ala?Ala?Cys?Pro?Tyr?Leu?Gln?Val?Ile?Ala?Leu?Pro?Tyr?Asn?Leu?Phe

295300305

gag?ggt?gtt?ttg?cca?cca?tgg?ctg?ggc?aag?ttg?acg?agt?ctt?aat?gcc 4255

Glu?Gly?Val?Leu?Pro?Pro?Trp?Leu?Gly?Lys?Leu?Thr?Ser?Leu?Asn?Ala

310315320

atc?tcc?ttg?ggt?tgg?aat?aac?ctt?gat?gct?ggc?ccg?atc?cct?act?gaa 4303

Ile?Ser?Leu?Gly?Trp?Asn?Asn?Leu?Asp?Ala?Gly?Pro?Ile?Pro?Thr?Glu

325 330 335 340

ctt?agc?aac?ctc?acc?atg?ctg?gca?gtc?tta?gat?ttg?tcg?acg?tgc?aac 4351

Leu?Ser?Asn?Leu?Thr?Met?Leu?Ala?Val?Leu?Asp?Leu?Ser?Thr?Cys?Asn

345 350 355

ctg?aca?gga?aac?atc?cct?gca?gat?att?ggg?cac?cta?ggc?caa?ctt?tca 4399

Leu?Thr?Gly?Asn?Ile?Pro?Ala?Asp?Ile?Gly?His?Leu?Gly?Gln?Leu?Ser

360 365 370

tgg?ttg?cat?ctt?gcg?agg?aat?caa?cta?aca?gga?cct?att?cct?gct?tct 4447

Trp?Leu?His?Leu?Ala?Arg?Asn?Gln?Leu?Thr?Gly?Pro?Ile?Pro?Ala?Ser

375 380 385

ctt?ggc?aac?ctt?tca?tcg?tta?gca?atc?ctg?cta?ttg?aaa?gga?aac?ttg 4495

Leu?Gly?Asn?Leu?Ser?Ser?Leu?Ala?Ile?Leu?Leu?Leu?Lys?Gly?Asn?Leu

390 395 400

ttg?gat?gga?tca?tta?cca?gcg?aca?gtt?gat?agc?atg?aac?tca?cta?act 4543

Leu?Asp?Gly?Ser?Leu?Pro?Ala?Thr?Val?Asp?Ser?Met?Asn?Ser?Leu?Thr

405 410 415 420

gca?gtt?gat?gtt?act?gaa?aac?aat?cta?cac?gga?gat?ctc?aac?ttc?ctt 4591

Ala?Val?Asp?Val?Thr?Glu?Asn?Asn?Leu?His?Gly?Asp?Leu?Asn?Phe?Leu

425 430 435

tct?act?gtt?tcc?aat?tgt?aga?aag?ctt?tct?acc?ctt?caa?atg?gac?ttt 4639

Ser?Thr?Val?Ser?Asn?Cys?Arg?Lys?Leu?Ser?Thr?Leu?Gln?Met?Asp?Phe

440 445 450

aat?tat?atc?acc?gga?agc?ctc?cca?gac?tat?gtt?ggg?aac?ctg?tcg?tca 4687

Asn?Tyr?Ile?Thr?Gly?Ser?Leu?Pro?Asp?Tyr?Val?Gly?Asn?Leu?Ser?Ser

455 460 465

cag?ctg?aaa?tgg?ttc?acg?tta?tct?aac?aac?aag?tta?act?ggc?acg?ctt 4735

Gln?Leu?Lys?Trp?Phe?Thr?Leu?Ser?Asn?Asn?Lys?Leu?Thr?Gly?Thr?Leu

470 475 480

cca?gct?acc?att?tca?aat?tta?act?ggt?ctt?gag?gtg?ata?gat?ctt?tcg 4783

Pro?Ala?Thr?Ile?Ser?Asn?Leu?Thr?Gly?Leu?Glu?Val?Ile?Asp?Leu?Ser

485 490 495 500

cat?aac?caa?ctg?cgc?aat?gca?att?cca?gaa?tca?atc?atg?acg?att?gag 4831

His?Asn?Gln?Leu?Arg?Asn?Ala?Ile?Pro?Glu?Ser?Ile?Met?Thr?Ile?Glu

505 510 515

aat?ctc?caa?tgg?ctt?gac?cta?agt?gga?aat?agc?ttg?tct?ggc?ttc?atc 4879

Asn?Leu?Gln?Trp?Leu?Asp?Leu?Ser?Gly?Asn?Ser?Leu?Ser?Gly?Phe?Ile

520 525 530

cca?tcg?aat?act?gca?ctt?cta?agg?aac?att?gta?aaa?cta?ttc?ctt?gaa 4927

Pro?Ser?Asn?Thr?Ala?Leu?Leu?Arg?Asn?Ile?Val?Lys?Leu?Phe?Leu?Glu

535 540 545

agc?aac?gaa?att?tct?ggc?tcc?ata?cca?aag?gac?atg?aga?aac?ctc?act 4975

Ser?Asn?Glu?Ile?Ser?Gly?Ser?Ile?Pro?Lys?Asp?Met?Arg?Asn?Leu?Thr

550 555 560

aat?cta?gag?cac?ctt?cta?ttg?tct?gat?aac?caa?tta?acg?tca?acc?gtg 5023

Asn?Leu?Glu?His?Leu?Leu?Leu?Ser?Asp?Asn?Gln?Leu?Thr?Ser?Thr?Val

565 570 575 580

cca?cca?agc?tta?ttt?cat?ctt?gat?aaa?atc?atc?agg?cta?gat?ctt?tct 5071

Pro?Pro?Ser?Leu?Phe?His?Leu?Asp?Lys?Ile?Ile?Arg?Leu?Asp?Leu?Ser

585 590 595

cga?aac?ttc?ttg?agt?ggt?gca?ctg?ccg?gtt?gat?gta?ggg?tac?ttg?aag 5119

Arg?Asn?Phe?Leu?Ser?Gly?Ala?Leu?Pro?Val?Asp?Val?Gly?Tyr?Leu?Lys

600 605 610

caa?att?acc?atc?ata?gat?ctc?tct?gac?aac?agc?ttt?tct?ggc?agc?atc 5167

Gln?Ile?Thr?Ile?Ile?Asp?Leu?Ser?Asp?Asn?Ser?Phe?Ser?Gly?Ser?Ile

615 620 625

cca?gat?tcg?ata?gga?gaa?ctt?cag?atg?tta?aca?cac?ctg?aat?cta?tca 5215

Pro?Asp?Ser?Ile?Gly?Glu?Leu?Gln?Met?Leu?Thr?His?Leu?Asn?Leu?Ser

630 635 640

gct?aac?gaa?ttc?tat?gat?tct?gtt?cca?gac?tct?ttt?ggt?aat?tta?act 5263

Ala?Asn?Glu?Phe?Tyr?Asp?Ser?Val?Pro?Asp?Ser?Phe?Gly?Asn?Leu?Thr

645 650 655 660

ggc?ttg?caa?act?ttg?gac?ata?tcc?cat?aac?aat?att?tct?ggt?acc?atc 5311

Gly?Leu?Gln?Thr?Leu?Asp?Ile?Ser?His?Asn?Asn?Ile?Ser?Gly?Thr?Ile

665 670 675

cca?aac?tac?ttg?gct?aat?ttt?acg?acc?ctt?gtt?agc?ttg?aac?cta?tct 5359

Pro?Asn?Tyr?Leu?Ala?Asn?Phe?Thr?Thr?Leu?Val?Ser?Leu?Asn?Leu?Ser

680 685 690

ttc?aat?aaa?cta?cat?ggt?caa?ata?ccg?gaa?gga?ggt?atc?ttt?gca?aac 5407

Phe?Asn?Lys?Leu?His?Gly?Gln?Ile?Pro?Glu?Gly?Gly?Ile?Phe?Ala?Asn

695 700 705

atc?act?tta?caa?tac?ttg?gtg?ggg?aac?tca?ggg?cta?tgt?ggt?gct?gcc 5455

Ile?Thr?Leu?Gln?Tyr?Leu?Val?Gly?Asn?Ser?Gly?Leu?Cys?Gly?Ala?Ala

710 715 720

cgt?tta?gga?ttc?cca?cca?tgc?caa?acc?acc?tcc?ccc?aag?aga?aat?ggt 5503

Arg?Leu?Gly?Phe?Pro?Pro?Cys?Gln?Thr?Thr?Ser?Pro?Lys?Arg?Asn?Gly

725 730 735 740

cac?atg?cta?aaa?tac?ttg?cta?ccg?act?ata?atc?ata?gta?gtt?gga?gtt 5551

His?Met?Leu?Lys?Tyr?Leu?Leu?Pro?Thr?Ile?Ile?Ile?Val?Val?Gly?Val

745 750 755

gta?gct?tgt?tgc?ttg?tat?gta?atg?att?aga?aag?aaa?gct?aac?cat?caa 5599

Val?Ala?Cys?Cys?Leu?Tyr?Val?Met?Ile?Arg?Lys?Lys?Ala?Asn?His?Gln

760 765 770

aag?att?tct?gct?ggt?atg?gct?gac?ctt?atc?agc?cat?caa?ttt?ctg?tcc 5647

Lys?Ile?Ser?Ala?Gly?Met?Ala?Asp?Leu?Ile?Ser?His?Gln?Phe?Leu?Ser

775 780 785

tat?cat?gag?ctt?ctt?cgt?gca?acc?gat?gat?ttc?agt?gat?gat?aac?atg 5695

Tyr?His?Glu?Leu?Leu?Arg?Ala?Thr?Asp?Asp?Phe?Ser?Asp?Asp?Asn?Met

790 795 800

ttg?ggc?ttc?gga?agc?ttt?gga?aaa?gtt?ttt?aag?gga?cag?ttg?agc?aac 5743

Leu?Gly?Phe?Gly?Ser?Phe?Gly?Lys?Val?Phe?Lys?Gly?Gln?Leu?Ser?Asn

805 810 815 820

ggt?atg?gtg?gtt?gcc?ata?aaa?gta?ata?cac?cag?cat?ctg?gaa?cat?gcc 5791

Gly?Met?Val?Val?Ala?Ile?Lys?Val?Ile?His?Gln?His?Leu?Glu?His?Ala

825 830 835

atg?aga?agc?ttt?gac?acc?gag?tgt?cgt?gta?ctc?cga?att?gct?cga?cat 5839

Met?Arg?Ser?Phe?Asp?Thr?Glu?Cys?Arg?Val?Leu?Arg?Ile?Ala?Arg?His

840 845 850

cgc?aac?ctg?ata?aag?att?ctg?aac?act?tgt?tcc?aac?ctg?gac?ttc?aga 5887

Arg?Asn?Leu?Ile?Lys?Ile?Leu?Asn?Thr?Cys?Ser?Asn?Leu?Asp?Phe?Arg

855 860 865

gca?ctc?gta?ctt?cag?tac?atg?ccc?aag?ggt?agc?tta?gaa?gca?ctc?ctg 5935

Ala?Leu?Val?Leu?Gln?Tyr?Met?Pro?Lys?Gly?Ser?Leu?Glu?Ala?Leu?Leu

870 875 880

cat?tca?gaa?caa?gga?aag?caa?tta?ggc?ttt?ctc?gag?agg?ttg?gat?att 5983

His?Ser?Glu?Gln?Gly?Lys?Gln?Leu?Gly?Phe?Leu?Glu?Arg?Leu?Asp?Ile

885 890 895 900

atg?cta?gat?gtg?tca?atg?gca?atg?gaa?tac?ctg?cat?cat?gag?cac?tat 6031

Met?Leu?Asp?Val?Ser?Met?Ala?Met?Glu?Tyr?Leu?His?His?Glu?His?Tyr

905 910 915

gag?gtg?gtc?tta?cac?tgc?gat?ttg?aag?cct?agc?aac?gta?cta?ttt?gac 6079

Glu?Val?Val?Leu?His?Cys?Asp?Leu?Lys?Pro?Ser?Asn?Val?Leu?Phe?Asp

920 925 930

gat?gat?atg?acg?gca?cat?gtg?gca?gac?ttt?ggc?att?gca?agg?ttg?ttg 6127

Asp?Asp?Met?Thr?Ala?His?Val?Ala?Asp?Phe?Gly?Ile?Ala?Arg?Leu?Leu

935 940 945

tta?ggt?gat?gac?aac?tcc?atg?atc?tca?gct?agc?atg?cca?gga?aca?gtt 6175

Leu?Gly?Asp?Asp?Asn?Ser?Met?Ile?Ser?Ala?Ser?Met?Pro?Gly?Thr?Val

950 955 960

ggg?tac?atg?gca?cca?g?gtacttagta?ctagtttttg?ttgtcttgct?caagcattgc 6231

Gly?Tyr?Met?Ala?Pro

965

tgatctttta?ttattatcaa?gtagggtgcg?actaattttt?ggtgactaac?ttttcttgag 6291

cag?ag tat?ggg?gct?cta?gga?aag?gcg?tca?cgg?aag?agc?gat?gtg?ttc 6338

Glu?Tyr?Gly?Ala?Leu?Gly?Lys?Ala?Ser?Arg?Lys?Ser?Asp?Val?Phe

975 980

agt?tac?ggg?atc?atg?ttg?ttt?gaa?gtg?ttc?act?ggg?aag?aga?ccc?aca 6386

Ser?Tyr?Gly?Ile?Met?Leu?Phe?Glu?Val?Phe?Thr?Gly?Lys?Arg?Pro?Thr

985 990 995 1000

gat?gct?atg?ttt?gtg gga?gaa?ctg?aac?atc agg?cag?tgg?gtt?cac 6431

Asp?Ala?Met?Phe?Val Gly?Glu?Leu?Asn?Ile Arg?Gln?Trp?Val?His

1005 1010 1015

cag?gcg?ttt?cct?gca gag?ctt?gtc?cat?gtg gtg?gac?tgc?caa?ctt 6476

Gln?Ala?Phe?Pro?Ala Glu?Leu?Val?His?Val Val?Asp?Cys?Gln?Leu

1020 1025 1030

cta?cat?gat?ggc?tct tct?tcc?agt?aac?atg cat?ggc?ttc?ctt?gtg 6521

Leu?His?Asp?Gly?Ser Ser?Ser?Ser?Asn?Met His?Gly?Phe?Leu?Val

1035 1040 1045

cca?gtg?ttc?gag?ctg ggc?ttg?ctc?tgc?tca gct?gac?tcc?cca?gac 6566

Pro?Val?Phe?Glu?Leu Gly?Leu?Leu?Cys?Ser Ala?Asp?Ser?Pro?Asp

1050 1055 1060

caa?agg?atg?gcg?atg agc?gat?gtg?gtc?gtg aca?ctg?aag?aag?att 6611

Gln?Arg?Met?Ala?Met Ser?Asp?Val?Val?Val Thr?Leu?Lys?Lys?Ile

1065 1070 1075

agg?aag?gac?tat?gtc aaa?ttg?atg?gca?acc aca?gag?aac?gct?gtg 6656

Arg?Lys?Asp?Tyr?Val Lys?Leu?Met?Ala?Thr Thr?Glu?Asn?Ala?Val

1080 1085 1090

cag?cag?tgattcatca?ctttcttgtg?gtatatgagc?gaatgaaatg?tatatccttt 6712

Gln?Gln

gcatccattt?cttcttctgc?attaggaaca?gcatcagtgc?atgcccagtg?atcgaataac 6772

ccttttgctt?ctatttgtgt?atggttgaat?tgaatatatc?tacggtgctt?caggttcagc 6832

aacaatttag?ttggtgtaaa?aatgtgattg?aactgctggt?cgataaattt?gcatcatgaa 6892

aatgaaaatg?ggagtagatg?atgtgctgct?tatattttcc?tatttctggc?caaatatata 6952

taaaaaagga?tattctcact?tgaaaacaga?atgaggtttg?ctttgtagac?attgggcctt 7012

tgtcgtgggc?ttctctggta?ccctgacatg?ctttatagcc?catgggccta?tgtttgtaat 7072

gggcttttgt?ttctttcgaa?tgactcaagg?tatattaagg?cctgtttgaa?cacttcggaa 7132

tattttgcgg?tggtagaggt?ggacactaaa?cgacgacata?ggtaaaatga?tatatagaaa 7192

caaaattaat?tcaaacgata?tactcatgct?ataaatttga?gatatggtaa?ttaataaaac 7252

ttcacatagg?caacgaaccg?tatatataac?agttaatgca?taataatggc?agagatttga 7312

taatgaatcc?tccaagattt?actcttgtga?ctctatataa?acagctgcaa?aatgaagcta 7372

ggtagtcagc?atatgcagta?gcttccttct?aaaagaagat?ctctcatctc?atcatggagc 7432

aacttctcaa?caagaaggct?gcagtgttct?tgttcatagc?tcttatggtg?atggctaccg 7492

taaatttctc?atcctgtcat?actacacaag?gtatatacct?ctggaattaa?tttctacatc 7552

acatccatat?atattgaaaa?cagtttaact?tgttctcgtt?tgcaatggct?ccatcacatc 7612

aatgaagcta?gaataatctg?atatagcctg?acaagtatat?gtgcatttta?cattttctgt 7672

atgaactatt?ttagctttct?gtaaaagtcg?ggtcattaac?aatataatga?tcttttctct 7732

aaaaacatgc?attttacatg?tgtatatata?taggtggata?tggagaaatg?gattcgtgca 7792

tggtccttga?acgttgcgat?atgaacaagt?gcatgagtgc?ctgccaagtc?aacaagtaca 7852

acggaggtca?gtgcgacggc?gagttgaacg?accactgctg?ctgtactgat?gaggccccgc 7912

acaaataata?gatttctctt?gtccaaatca?acgacgacgg?ttgtataatc?aacggcaatc 7972

ttattattga?ctgatgcaga?gattccataa?taaacttgca?gttgatgctt?gtaccaattc 8032

actcagtatg?tcaattagta?aaaggagaat?cccacgcaaa?tgtgcgggca?acttatttat 8092

tctttattaa?aagaataaat?tttatttaaa?gactgtagat?tatcttatgt?cattaaaaaa 8152

gaaatatctc?tatcctgaaa?ttgatgatag?actttgatac?actaaactaa?cttataagag 8212

tatctctcat?attaaaaaaa?gatcactatg?tcttggttat?atataaactc?tcctatcaca 8272

actacttatt?attctctcga?aaaagctaag?tacgttttta?atagatagaa?ttgcttatta 8332

aaaaacaaac?ataatacatc?attaatgcaa?taaaaaaatc?atctctattt?cattccgaag 8392

tgcacatgtc?tcacttgtgt?ctgaatgtat?aaatatttta?aaatgccatt?tctgtcaatt 8452

aatgaaagaa?aaccattaaa?attagatata?aatacccatg?taatgtgtaa?agttggtcat 8512

agctgttaag?gctttattct?ctaatgcctt?gaaatatggg?tcctgcattg?agccatgccc 8572

ctcaagacta?cgtcgatcat?aatttttaac?acctagctca?ctctctacac?atttataaag 8632

aattgtatca?gtaagtaggc?tagttgagct?ctacctccaa?gagtgaaaga?agtcataggg 8692

gattcgatgg?actgatccta?tgtatgtgtg?tacaaacata?ataacatttt?agttttaaat 8752

ttgaggatta?ggagggaaag?attaatattt?tttcaattgt?acatgcatac?atatattttt 8812

tctagggttt?tggatgaaca?gtacttattt?ttctgtggta?gtggtactta?catgggagat 8872

taacagggaa?ggattacttt?aatacatcta?atcaaatatt?ttaaaataat?gggtatattg 8932

gtttaagtat?aaatcaatgg?tagatgtttt?gcttttttct?catatttttt?tatattttca 8992

ccaatttatt?agagcatcag?gttgttgcct?aaggagcgtt?tgtagagatt?aggtgggagt 9052

gattagttta?acagatctaa?tctaataatt?aaaaatattg?ggtcaaccca?tttaagtata 9112

atcaatcgct?agatgtttta?ctttttttta?gaatttcttg?gatttttcct?aatttattag 9172

agcaccatgt?gacggcttaa?gattgtttgt?agcagtgcca?catggtggct?tagaagcgtt 9232

tgtatgatgt?tcaatggact?tttagtatat?aataatattt?ttgtctcaat?gattggtgga 9292

tagcactgca?tatgtagaat?tgatacatga?gttcgagtct?gacataaaat?taacacataa 9352

ggtaagacat?gcatgagtcg?aatacattgg?atcttattta?ttatataccc?agattaactt 9412

aagtttaaac?tgcagagact?gaagaggaaa?agatcaacat?atatgcctcc?tgttactctt 9472

aattaagaca?tatcgatccg?gttcatttaa?tcatttattt?tctatagtcc?tttacaatat 9532

atctagattt?atattactgt?gataattgat?attttaaagc?gaagataata?ttttttaatg 9592

gtaatcacaa?ttagagtatt?ttagtttgga?acattgtaga?ttgctttaaa?tattaaagtt 9652

atgatgatgt?caatcatgaa?agatttaagt?aaagttattt?tgttttgtga?aaaataatat 9712

tgttttaaca?aataaatatt?ttagaacagt?ggtattattt?tttgggagtg?ctaatcacaa 9772

ctaattagaa?tattttattt?tgtgatatta?ttgagctaat?ctaattatgc?atgattgtga 9832

aatgatatat?gatgatgtga?tctacattat?ctattttctt?cacaaccttt?tttattgttg 9892

ggatgacatg?caaaaggttc?ggatatgatt?taaaatacta?gaaggaatat?tttttggaac 9952

gtcactcacg?atttaagtgt?ttttgttttt?tttcatatct?atcatattta?atttatttgt 10012

gcatgtttta?taaagtgata?tatgatcata?taatatacgt?tgtcattttt?ttatttaaca 10072

gcttcaattg?catgcttagg?ttaggatgtg?atgaaaatta?aatatttttt?tatgagttaa 10132

agaatgtagt?ggcttatatg?ggtatttgaa?tggttaatta?agcatcatga?atacaattat 10192

aaagaatgat?aaaaagattt?gtgtgtaatt?taaagattat?gattatttct?ctatacggaa 10252

attatttaaa?aatattattt?ttaattgtaa?caacttaaaa?gaaaaaaaga?gatgagaaga 10312

cttataggag?ggggagggat?acgtacatac?actatgggag?ggggaggggg?gtcggctggt 10372

ggggggagag?gggagggagg?tgtattgtgg?ggttttttta?aaaaaaataa?atctaataga 10432

taaatatagt?aggcctaccg?gtttattaga?gagtcatatg?acaacttaga?agcatttata 10492

gaagccccac?gtgacggctt?gagaatgttt?agtagaagtt?taatgaactt?ttagtatata 10552

atagataaat?taattaatga?tgatgaggtt?ggcctgcctt?ggcttctctc?atgttgattt 10612

actattgtgt?atggcccatg?tatatgaaaa?ggcaaaatgt?agcaatgagt?cggtttgact 10672

taaaacatgc?gggtttaaaa?tgacatgctc?tgtaaccgat?gagagtactc?agtttaagtt 10732

aaggtaacaa?atcaatatta?caagcagaaa?tacagatgca?agtggtacga?acaaacggtt 10792

ttttattcta?attattttca?aattcagttt?tcaaagtaat?agtgtttatt?tgatctggaa 10852

tttcataggg?atgagcaatc?ttaagccaaa?tatcataatc?ttgagccata?atgtaagatt 10912

acatcttgtc?acaccaataa?ggaaaatcga?caccatcgaa?aacatgtggc?ttggttgaaa 10972

acttgttagc?catgacttaa?aactttagat?cggtaaaaat?ccaaagaaga?aaacgaggct 11032

ctgataccac?ttgtaggatc?gaatcccaaa?taccaagcca?accagagggg?gtgaatgtat 11092

cctattgtcc?ctggcatgct?ggctgtaact?gcagagttgt?catcacctaa?cagtaatttt 11152

gcaatgccaa?agtccgccac?atgtgccgtg?atttcctcat?caaacagcac?attgctaggc 11212

ttgaggtcac?agtactgcaa?aacaacctca?gaatgatgat?agtgaaggtg?ctccattgcc 11272

attgatacgt?ccagcataat?gtcaagtctc?tttaggaatc?ctagtggagg?gtgcccttcc 11332

ttgtgcaggt?atgtctccaa?gctgccattg?ggcatgtact?gaagcagcag?tgctttgaag 11392

tctatgttgg?agcatatgtt?tagtatcctt?atcaagttgc?gatgtcgagc?catgcgtaga 11452

acctgacact?caacatcaaa?gcttctcata?gcttgttcct?cttgcatgtt?cagaacttta 11512

atcgcaacca?ccattccatc?atccaaatga?cccttgaaaa?ctttgccaaa?acttccagtt 11572

cccaacatat?tattctcatt?gaaactttct?gtggcacgaa?caatctcttc?atatgatact 11632

aacctataag?aagtaggtgt?agtaatatct?agtttctttt?taatcttctt?tctagtcatt 11692

tggtataaac?aaagagcaag?tgcgccaact?gctattgtga?tggcagggag?tataaacttc 11752

aggtagtggt?taccattagt?tgagtgtgac?ttgtttgggc?atggtgaaaa?tcctagacga 11812

ggaagaccac?acaaggcagc?attgcccatc?aaagat 11848

 

<210>2

<211>1092

<212>PRT

＜213〉paddy rice (Oryza sativa)

<400>2

Met?Ala?Leu?Gly?Leu?Pro?Val?Trp?Ile?Phe?Val?Ala?Leu?Leu?Ile?Ala

1 5 10 15

Leu?Ser?Thr?Val?Pro?Cys?Ala?Ser?Ser?Leu?Gly?Pro?Ser?Lys?Ser?Asn

20 25 30

Gly?Ser?Asp?Ile?Asp?Leu?Ala?Ala?Leu?Leu?Ala?Leu?Lys?Ser?Gln?Phe

35 40 45

Ser?Asp?Pro?Asp?Asn?Ile?Leu?Ala?Gly?Asn?Trp?Thr?Ile?Gly?Thr?Pro

50 55 60

Phe?Cys?Gln?Trp?Met?Gly?Val?Ser?Cys?Ser?His?Arg?Arg?Gln?Arg?Val

65 70 75 80

Thr?Ala?Leu?Lys?Leu?Pro?Asn?Val?Pro?Leu?Gln?Gly?Glu?Leu?Ser?Ser

85 90 95

His?Leu?Gly?Asn?Ile?Ser?Phe?Leu?Leu?Ile?Leu?Asn?Leu?Thr?Asn?Thr

100 105 110

Gly?Leu?Thr?Gly?Leu?Val?Pro?Asp?Tyr?Ile?Gly?Arg?Leu?Arg?Arg?Leu

115 120 125

Glu?Ile?Leu?Asp?Leu?Gly?His?Asn?Ala?Leu?Ser?Gly?Gly?Val?Pro?Ile

130 135 140

Ala?Ile?Gly?Asn?Leu?Thr?Arg?Leu?Gln?Leu?Leu?Asn?Leu?Gln?Phe?Asn

145 150 155 160

Gln?Leu?Tyr?Gly?Pro?Ile?Pro?Ala?Glu?Leu?Gln?Gly?Leu?His?Ser?Leu

165 170 175

Asp?Ser?Met?Asn?Leu?Arg?His?Asn?Tyr?Leu?Thr?Gly?Ser?Ile?Pro?Asp

180 185 190

Asn?Leu?Phe?Asn?Asn?Thr?Ser?Leu?Leu?Thr?Tyr?Leu?Asn?Val?Gly?Asn

195 200 205

Asn?Ser?Leu?Ser?Gly?Pro?Ile?Pro?Gly?Cys?Ile?Gly?Ser?Leu?Pro?Ile

210 215 220

Leu?Gln?Tyr?Leu?Asn?Leu?Gln?Ala?Asn?Asn?Leu?Thr?Gly?Ala?Val?Pro

225 230 235 240

Pro?Ala?Ile?Phe?Asn?Met?Ser?Lys?Leu?Ser?Thr?Ile?Ser?Leu?Ile?Ser

245 250 255

Asn?Gly?Leu?Thr?Gly?Pro?Ile?Pro?Gly?Asn?Thr?Ser?Phe?Ser?Leu?Pro

260 265 270

Val?Leu?Gln?Trp?Phe?Ala?Ile?Ser?Lys?Asn?Asn?Phe?Phe?Gly?Gln?Ile

275 280 285

Pro?Leu?Gly?Leu?Ala?Ala?Cys?Pro?Tyr?Leu?Gln?Val?Ile?Ala?Leu?Pro

290 295 300

Tyr?Asn?Leu?Phe?Glu?Gly?Val?Leu?Pro?Pro?Trp?Leu?Gly?Lys?Leu?Thr

305 310 315 320

Ser?Leu?Asn?Ala?Ile?Ser?Leu?Gly?Trp?Asn?Asn?Leu?Asp?Ala?Gly?Pro

325 330 335

Ile?Pro?Thr?Glu?Leu?Ser?Asn?Leu?Thr?Met?Leu?Ala?Val?Leu?Asp?Leu

340 345 350

Ser?Thr?Cys?Asn?Leu?Thr?Gly?Asn?Ile?Pro?Ala?Asp?Ile?Gly?His?Leu

355 360 365

Gly?Gln?Leu?Ser?Trp?Leu?His?Leu?Ala?Arg?Asn?Gln?Leu?Thr?Gly?Pro

370 375 380

Ile?Pro?Ala?Ser?Leu?Gly?Asn?Leu?Ser?Ser?Leu?Ala?Ile?Leu?Leu?Leu

385 390 395 400

Lys?Gly?Asn?Leu?Leu?Asp?Gly?Ser?Leu?Pro?Ala?Thr?Val?Asp?Ser?Met

405 410 415

Asn?Ser?Leu?Thr?Ala?Val?Asp?Val?Thr?Glu?Asn?Asn?Leu?His?Gly?Asp

420 425 430

Leu?Asn?Phe?Leu?Ser?Thr?Val?Ser?Asn?Cys?Arg?Lys?Leu?Ser?Thr?Leu

435 440 445

Gln?Met?Asp?Phe?Asn?Tyr?Ile?Thr?Gly?Ser?Leu?Pro?Asp?Tyr?Val?Gly

450 455 460

Asn?Leu?Ser?Ser?Gln?Leu?Lys?Trp?Phe?Thr?Leu?Ser?Asn?Asn?Lys?Leu

465 470 475 480

Thr?Gly?Thr?Leu?Pro?Ala?Thr?Ile?Ser?Asn?Leu?Thr?Gly?Leu?Glu?Val

485 490 495

Ile?Asp?Leu?Ser?His?Asn?Gln?Leu?Arg?Asn?Ala?Ile?Pro?Glu?Ser?Ile

500 505 510

Met?Thr?Ile?Glu?Asn?Leu?Gln?Trp?Leu?Asp?Leu?Ser?Gly?Asn?Ser?Leu

515 520 525

Ser?Gly?Phe?Ile?Pro?Ser?Asn?Thr?Ala?Leu?Leu?Arg?Asn?Ile?Val?Lys

530 535 540

Leu?Phe?Leu?Glu?Ser?Asn?Glu?Ile?Ser?Gly?Ser?Ile?Pro?Lys?Asp?Met

545 550 555 560

Arg?Asn?Leu?Thr?Asn?Leu?Glu?His?Leu?Leu?Leu?Ser?Asp?Asn?Gln?Leu

565 570 575

Thr?Ser?Thr?Val?Pro?Pro?Ser?Leu?Phe?His?Leu?Asp?Lys?Ile?Ile?Arg

580 585 590

Leu?Asp?Leu?Ser?Arg?Asn?Phe?Leu?Ser?Gly?Ala?Leu?Pro?Val?Asp?Val

595 600 605

Gly?Tyr?Leu?Lys?Gln?Ile?Thr?Ile?Ile?Asp?Leu?Ser?Asp?Asn?Ser?Phe

610 615 620

Ser?Gly?Ser?Ile?Pro?Asp?Ser?Ile?Gly?Glu?Leu?Gln?Met?Leu?Thr?His

625 630 635 640

Leu?Asn?Leu?Ser?Ala?Asn?Glu?Phe?Tyr?Asp?Ser?Val?Pro?Asp?Ser?Phe

645 650 655

Gly?Asn?Leu?Thr?Gly?Leu?Gln?Thr?Leu?Asp?Ile?Ser?His?Asn?Asn?Ile

660 665 670

Ser?Gly?Thr?Ile?Pro?Asn?Tyr?Leu?Ala?Asn?Phe?Thr?Thr?Leu?Val?Ser

675 680 685

Leu?Asn?Leu?Ser?Phe?Asn?Lys?Leu?His?Gly?Gln?Ile?Pro?Glu?Gly?Gly

690 695 700

Ile?Phe?Ala?Asn?Ile?Thr?Leu?Gln?Tyr?Leu?Val?Gly?Asn?Ser?Gly?Leu

705 710 715 720

Cys?Gly?Ala?Ala?Arg?Leu?Gly?Phe?Pro?Pro?Cys?Gln?Thr?Thr?Ser?Pro

725 730 735

Lys?Arg?Asn?Gly?His?Met?Leu?Lys?Tyr?Leu?Leu?Pro?Thr?Ile?Ile?Ile

740 745 750

Val?Val?Gly?Val?Val?Ala?Cys?Cys?Leu?Tyr?Val?Met?Ile?Arg?Lys?Lys

755 760 765

Ala?Asn?His?Gln?Lys?Ile?Ser?Ala?Gly?Met?Ala?Asp?Leu?Ile?Ser?His

770 775 780

Gln?Phe?Leu?Ser?Tyr?His?Glu?Leu?Leu?Arg?Ala?Thr?Asp?Asp?Phe?Ser

785 790 795 800

Asp?Asp?Asn?Met?Leu?Gly?Phe?Gly?Ser?Phe?Gly?Lys?Val?Phe?Lys?Gly

805 810 815

Gln?Leu?Ser?Asn?Gly?Met?Val?Val?Ala?Ile?Lys?Val?Ile?His?Gln?His

820 825 830

Leu?Glu?His?Ala?Met?Arg?Ser?Phe?Asp?Thr?Glu?Cys?Arg?Val?Leu?Arg

835 840 845

Ile?Ala?Arg?His?Arg?Asn?Leu?Ile?Lys?Ile?Leu?Asn?Thr?Cys?Ser?Asn

850 855 860

Leu?Asp?Phe?Arg?Ala?Leu?Val?Leu?Gln?Tyr?Met?Pro?Lys?Gly?Ser?Leu

865 870 875 880

Glu?Ala?Leu?Leu?His?Ser?Glu?Gln?Gly?Lys?Gln?Leu?Gly?Phe?Leu?Glu

885 890 895

Arg?Leu?Asp?Ile?Met?Leu?Asp?Val?Ser?Met?Ala?Met?Glu?Tyr?Leu?His

900 905 910

His?Glu?His?Tyr?Glu?Val?Val?Leu?His?Cys?Asp?Leu?Lys?Pro?Ser?Asn

915 920 925

Val?Leu?Phe?Asp?Asp?Asp?Met?Thr?Ala?His?Val?Ala?Asp?Phe?Gly?Ile

930 935 940

Ala?Arg?Leu?Leu?Leu?Gly?Asp?Asp?Asn?Ser?Met?Ile?Ser?Ala?Ser?Met

945 950 955 960

Pro?Gly?Thr?Val?Gly?Tyr?Met?Ala?Pro?Glu?Tyr?Gly?Ala?Leu?Gly?Lys

965 970 975

Ala?Ser?Arg?Lys?Ser?Asp?Val?Phe?Ser?Tyr?Gly?Ile?Met?Leu?Phe?Glu

980 985 990

Val?Phe?Thr?Gly?Lys?Arg?Pro?Thr Asp?Ala?Met?Phe?Val Gly?Glu?Leu

995 1000 1005

Asn?Ile Arg?Gln?Trp?Val?His Gln?Ala?Phe?Pro?Ala Glu?Leu?Val

1010 1015 1020

His?Val Val?Asp?Cys?Gln?Leu Leu?His?Asp?Gly?Ser Ser?Ser?Ser

1025 1030 1035

Asn?Met His?Gly?Phe?Leu?Val Pro?Val?Phe?Glu?Leu Gly?Leu?Leu

1040 1045 1050

Cys?Ser Ala?Asp?Ser?Pro?Asp Gln?Arg?Met?Ala?Met Ser?Asp?Val

1055 1060 1065

Val?Val Thr?Leu?Lys?Lys?Ile Arg?Lys?Asp?Tyr?Val Lys?Leu?Met

1070 1075 1080

Ala?Thr Thr?Glu?Asn?Ala?Val Gln?Gln

1085 1090

Claims (3)

1. one kind isolatingly produces the gene Xa3/Xa26-2 of resistance to bacterial leaf-blight, and its nucleotide sequence is shown in sequence table SEQ IDNO:1.

2. one kind isolatingly produces the gene Xa3/Xa26-2 of resistance to bacterial leaf-blight, and its encoding sequence is shown in 3284-6191 and 6295-6662 position among the sequence table SEQ IDNO:1.

3. claim 1 or 2 described genes are increasing paddy rice to the application in the bacterial leaf spot resistance.

Images