CN101874751A - Multi-layer porous scaffold and preparation method thereof - Google Patents
Multi-layer porous scaffold and preparation method thereof Download PDFInfo
- Publication number
- CN101874751A CN101874751A CN2009100504996A CN200910050499A CN101874751A CN 101874751 A CN101874751 A CN 101874751A CN 2009100504996 A CN2009100504996 A CN 2009100504996A CN 200910050499 A CN200910050499 A CN 200910050499A CN 101874751 A CN101874751 A CN 101874751A
- Authority
- CN
- China
- Prior art keywords
- layer
- solvent
- particle
- support
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention belongs to the technical field of biological materials and tissue repair and particularly relates to a multi-layer porous scaffold and a preparation method thereof. A suitable porous material is selected, after being clipped according to set size and requirements, the material is bonded by pore-forming adhesive which contains mixture of components of polymer/pore-forming particles/solvent, and pore-forming agent is removed after solidification and bonding, thus preparing the porous scaffold which has multi-layer structures communicated with each other. The scaffold has multi-layer structures, porous transition layers are among the layers of the scaffold, and the pores in an upper layer and a lower layer of the scaffold are communicated with each other; and the scaffold is suitable for transition of different cells and fusion of tissues in a process of tissue remodeling, and weakened separation between layers of the remolding tissue is avoided. The scaffold is suitable for a bionic three-dimensional cell scaffold which repairs a multi-layer tissue and other application fields.
Description
Technical field
The invention belongs to biomaterial and tissue repair technical field, be specifically related to a kind of multiwalled porous support and preparation method thereof.
Background technology
Porous support can be widely used in fields such as tissue repair, cell culture vector, wound dressing, medicine controlled release carrier.Especially the polyalcohol stephanoporate three-dimensional rack that has high porosity has developed into the important component part of organizational project and original position tissue regeneration.
The ultimate principle that prior art discloses organizational project is: extract certain normal cell from tissue, after cultivating propagation, make up the cell material complex at external and degradable multiporous support, again with this damaged place of species complex implanting tissue, along with the continuous secretion of material degradation and extracellular matrix and the continuous formation of tissue, rebuild new tissue and the organ that adapts with self form and function, reach the purpose of tissue repair.Therefore porous support has considerable status in the tissue reconstruction process.
In addition, directly implant porous support, utilize intravital cell voluntarily the inductive technology (or claim original position tissue regeneration) of organizing of repair tissue also depend on suitable porous support materials.
About tissue engineering bracket or organize the making and the forming technique of induction rack, relatively abundanter approach and means have been arranged at present.For example, fibrous woven technology, particle remove technology, gas foaming technology, phase detachment technique, microsphere sintering technology etc.The tissue engineering bracket that different biomaterials and preparation means obtain, important indicators such as its pore morphology, porosity, specific surface area and degradation rate are all variant.For example, the nonwoven fabric construct that electrostatic spinning obtains is suitable for the structure of thin layer tissue such as skin heart; Particle is removed the three-dimensional rack that obtains high porosity, can be applicable to bone and cartilage tissue engineered; Utilize the agglomerating method of microsphere, can obtain high-intensity support, but voidage is not high, be suitable for the reparation of osseous tissue.Therefore and in the tissue reconstruction process, the construction features of support can have influence on the 26S Proteasome Structure and Function of cambium, the porous support that needs pore structure to adapt with it at the reconstruction of different tissues.
The human organ more complicated is not by single organizational composition, and needs to adopt the focus of organizational project reparation clinically, often includes the serious defect of multilayer tissue.The form of different tissues, structure, mechanical property have very big difference, and different classes of growth and proliferation of cell and tissue reconstruction speed are also variant.Therefore just need be according to the characteristics of these defective tissues, the timbering material that design different porosities, pore structure and degradation rate combine.In addition, in the organizational project practice process, even if to histioid reparation, also need to consider its with surrounding tissue between cooperate.For example, in the clinical example of repair of cartilage, except realizing the reparation of cartilaginous tissue, the problem that is connected to become key of the cartilage of reconstruction and lower floor's osseous tissue.Otherwise cartilage regeneration is connected the weak link that will become tissue repair with interface between the bone matrix.In addition, in the reparation of tissues such as skin, long dried bone, all need to make up the complicated bionical tissue of multiple form.And described single support pore moulding process before relying on generally can only prepare single support of planting material, single pore structure.In the face of the clinical demand of the structure of complex organization the time, it is independent adjustable and the demand support that still is interconnected on the whole is bigger to have a multiple structure similar to defective tissue, each layer performance.
In order to obtain to make up the multiple structure that adapts with complex organization, consider from the angle of tissue repair, need to have good connectedness between the multiwalled porous support sometimes.Be communicated with if two previously prepared porous supports connect to be easy to cause lack between the support simply.
Summary of the invention
The object of the present invention is to provide a kind of multi-layer porous scaffold and preparation method thereof.Be specifically related to a kind of by the multilamellar support bonding and layer with layer between have the multi-layer porous scaffold of good intercommunicating pore structure.
The invention provides a kind of connective multiwalled porous support that possesses.The present invention adopts a kind of binding agent that contains the pore particle, and it is independent adjustable to prepare between a kind of layer and the layer structure, and layer with layer between the timbering material that is communicated with of hole maintenance.Its core is the porous material that select to be fit to, after directly using or cutting out on request, but bonding by the binding agent of pore, remove porogen after the curing, make porous support be interconnected multiple structure and complex appearance.
Particularly, multi-layer porous scaffold provided by the invention is characterized in that, has multiple structure, and each interlayer that constitutes rack body contains porous transition zone, makes and be interconnected between the hole of levels.Each layer can be adopt particle stripping, fibrous woven, be separated, the porous support of any one or a few technology preparation in gas foaming or the microsphere sintering etc.One or more layers the aperture of forming this support is 10-900 μ m, porosity 20%-99%, pore structure pattern of each layer and porosity are independent adjustable in the compound rest, the aperture of different layers and porosity can identical, also can be inequality, and the binding agent that contains porogen by use between each layer of support connects.
Among the present invention, adopt the mixture of forming by polymer, solvent and pore particle as binding agent.Behind applying adhesive on the adhesive surface between layer and the layer, adhesive surface is harmonious, exert pressure.Solvent in the binding agent is partly dissolved fusion with support top layer hole wall by diffusion, treat solvent evaporates after, adhesive layer is fixed, the polymer of different layers just couples together by intermediary adhesive layer.The method that adopts particle to remove at last with the particle stripping of adhesive layer, promptly obtains the porous compound support frame that layer and interlayer are interconnected.
The multi-layer porous scaffold that the present invention proposes it is characterized in that adopting the transition zone with loose structure that separate porous support layer and layer are connected into integral body, and the hole between layer and the layer has connectedness.Articulamentum thickness is generally about 0.5mm.
The host material of forming each layer support comprises macromolecular material, inorganic material, bio-derived material or the complex between them, and the host material between the different layers can identical, also can be inequality.
Described host material is to have from cementability, resolvability, plastic natural or synthesized polymer material, has both comprised degradable macromolecule, also comprises nondegradable macromolecular material.The host material of each layer support also comprises the complex between copolymer between the macromolecular material or blend and macromolecular material and bio-derived material, inorganic material etc.
Among the present invention, the macromolecular material of forming the porous support main body comprises degradable polymers such as polylactic acid, polyglycolic acid, poly butyric ester, polycaprolactone, Merlon, poe, poly-anhydride, poly-dioxane, chitosan, alginate, hyaluronate, collagen, gelatin, fibroin albumen, and any by two or more copolymer formed among them or blend; Also can be non-degradable polymer such as polystyrene, polrvinyl chloride, polyacrylate, polymethacrylates, Merlon, nylon, polyurethane, polyformaldehyde, polyvinyl alcohol, polyvinyl acetate, polysiloxanes, and any by two or more copolymer formed among them or blend.
Binding agent of the present invention is characterized in that being made up of polymer, solvent and pore particle.Polymers compositions wherein can be degradable polylactic acid, polyglycolic acid, poly butyric ester, polycaprolactone, Merlon, poe, poly-anhydride, poly-dioxane, chitosan, alginate, hyaluronate, collagen, gelatin, fibroin albumen and the copolymer of two or more composition or blend any among them; Also can be nondegradable polystyrene, polrvinyl chloride, polyacrylate, polymethacrylates, Merlon, nylon, polyurethane, polyformaldehyde, polyvinyl alcohol, polyvinyl acetate, polysiloxanes, and any by two or more copolymer formed among them or blend.
Solvent composition in the binding agent (being solvent A), can be acetone, butanone, chloroform, dichloromethane, oxolane, benzene,toluene,xylene, ethylene glycol, Ketohexamethylene, dioxane, N, any or several mixture in dinethylformamide, formic acid, benzyl alcohol, the cyclohexane extraction.Described solvent orange 2 A has fine solubility to binder polymer component and rack body material, but can not change its structure and performance; The pore particle is not dissolved and do not change its structure and character; Have suitable volatility, under vacuum condition, can remove fully.
Among the present invention, solvent B is insoluble or insoluble substantially to polymer, the pore particle there is good dissolving ability, and has suitable volatility, under vacuum condition, can be removed fully, adopted any or several mixture in pentane, hexane, heptane, octane, cyclohexane extraction or the water usually.
Among the present invention, the pore particle in the binding agent is dissolved in solvent B, but is insoluble to solvent orange 2 A.Described pore particle comprises inorganic salt, polysaccharide, organic micromolecule compound or the mixture of being made up of them, and particle can be that the ball-type of rule also can be irregular polyhedron, and particle diameter is in the 10-900 mu m range.The shared volume fraction of solvent orange 2 A is generally 20%-70% in the binding agent, and preferable scope is 30%-50%; The amount ranges of pore particle is the 20wt%-99wt% of mixture, and preferable scope is 70wt%-95wt%.
The present invention adopts the method for adhesives to prepare described multi-layer porous scaffold, by following step,
1, the solvent (solvent orange 2 A) of polymer raw material, polymer and pore particle are mixed after, obtain the binding agent of multilamellar support;
2, each ingredient of the porous support that will make is in advance directly used or is suitably cut out to obtain neat clean adhesive surface according to the tissue defect form;
3, the support binding agent is coated on the adhesive surface of each layer support, forms articulamentum, will treat that then bonding another layer support is placed on above, and exert pressure, treat to finalize the design after the solvent evaporates;
4, after typing is finished after a while, remove pressure, add new shelf layer as required, can repeat previous action;
5, interconnective multilamellar support is implemented the particle removing process, with the particle stripping of solvent B with articulamentum; Behind the dry removal residual solvent, make MULTILAYER COMPOSITE porous support with the pore structure of being interconnected.
Among the present invention, can earlier polymeric material be dissolved in the solvent orange 2 A when obtaining binding agent, form polymer solution, then the pore particle be joined in the macromolecular solution, mix.
Among the present invention, the moulding process of each layer support can adopt particle pore, non-woven fabrics braiding, foaming, is separated, in 3 D-printing and the particles sintering technology any one or a few.
When support of the present invention is bonding, need give certain pressure and the time chien shih adhesive layer solidify.Can implement on small-sized molding apparatus, prerequisite is to destroy the structure of each layer support, is generally a few minutes to a few hours hardening time not wait, and is decided by the volatilization ability of use solvent orange 2 A.
The present invention need make solvent orange 2 A remove after obtaining multilamellar support after bonding, can under the air at room temperature environment solvent orange 2 A partly be volatilized earlier, removes fully under the vacuum condition room temperature then, generally at 12-48 hour.Solvent B should be excessive greatly, is generally 50-1000 times of binding agent use amount.
Among the present invention, the above-mentioned compound rest that has removed particle is taken out, make most of solvent evaporates under the room temperature in the air, change over to then and remove residual solvent B under the vacuum condition.This process generally continues 12-48 hour, and vacuum drying oven is no more than the glass transition temperature of polymer, and general room temperature state gets final product.
The prepared multi-layer porous scaffold of the present invention is applicable to as organizational projects such as skin, bone/cartilage, blood vessel, esophagus, tracheas repairs employed cytoskeleton, is used for the artificial constructed of tissues such as skin, bone/cartilage, blood vessel, esophagus, trachea.
The present invention compares with existing compound rest technology of preparing, has following advantage and characteristics:
1, prepared multi-layer porous scaffold layer with the layer between be connected by the adhesive layer transition, different with common chemical adhesive, the adhesive layer that the present invention finally obtains also has loose structure, and and realize between each layer of support being interconnected, be suitable for the fusion between transition of different cells in the tissue reconstruction process and tissue, avoided rebuilding organized layer with layer between weakened separation.
2, prepared support, different layers can be different materials, different pore technology, also can be same moulding process different porosities support, and above combination arbitrarily, this flexible and changeable system all has practical significance on bionical two general orientation of 26S Proteasome Structure and Function.
3, the polymers compositions of the binding agent of the present invention's use is the material with excellent biological compatibility, can select and the rack body identical materials, also can select other suitable tissue engineering material,
4, the present invention can also pass through bonding technology, by the method that each several part splices and combines, makes the large-scale porous support of complex contour.
5, can avoid residues of harmful substances to greatest extent in the preparation method involved in the present invention, be fit to the manufacturing of medical embedded device.
For the ease of understanding, below will describe in detail of the present invention by concrete drawings and Examples.It needs to be noted, instantiation and accompanying drawing only are in order to illustrate, obviously those of ordinary skill in the art can illustrate according to this paper, within the scope of the invention the present invention is made various corrections and change, and these corrections and change are also included in the scope of the present invention.
Description of drawings
Fig. 1 is application principle (with bone/repair of cartilage the is example) sketch map of multilamellar support on organizational project, wherein the 1, the 3rd, and the polylactic acid of two-layer different degradation cycles (PLA) porous support, 2 is porous tack coat part.
Fig. 2 is aperture 350-455 μ m, the polylactic acid of porosity 90% and copolymer (PLGA) the porous support scanning electron microscope picture of polyglycolic acid by adopting the paraffin ball to obtain as the pore particle.
Fig. 3 adopts adhesive bonding method the bonding back of the PLGA porous support of two spherical pore structures to be formed the adhesive interface stereoscan photograph of compound rest, binding agent is made up of PLGA/ salt particle/dichloromethane, and the irregular porous zone about middle wide 400 μ m is an adhesive layer.Aperture 350-455 μ m, porosity 90%.
Fig. 4 is the outward appearance photo that bonding front and back porosity reaches 90% PLGA support, and a is two independent racks before bonding, and b is the two-layer compound support that bonding back forms.
The specific embodiment
Embodiment 1
At first prepare salt grain and spherical paraffin pore particle.The salt grain uses machinery to smash the back by the standard screen screening, collects the particle of different-grain diameter scope.The paraffin microsphere uses 1.0g gelatin, 20.0g paraffin, joins in the 400ml deionized water, is heated to 80 ℃ under the 400rpm mechanical agitation, adds 300ml frozen water quenching then, and the wax ball is solidified.Filter, with deionized water rinsing twice, screening is shone with standard in dry back, collects the particle of different-grain diameter scope.
Then prepare binding agent.With the 0.5g molecular weight is that 300,000 PLGA85/15 is dissolved in the 8.0ml dichloromethane, and the sodium chloride salt particle of 9.5g particle diameter 350-455 μ m is joined in the macromolecular solution mixing and stirring.
Get two column PLGA85/15 porous supports (high 5mm, diameter 10mm, porosity 90%, aperture 350-455 μ m) with the pore of paraffin microsphere, the end equating is whole.Binding agent is coated in equably on the bonding plane of a support wherein, the bonding plane with another piece support is buckled on the rack surface that has applied binding agent then.Put in the mold pressing utensil, apply the pressure of 40N, kept 6 hours.Pressure relief is taken out support, continues solvent flashing after 12 hours in air, changes vacuum under the room temperature over to greater than dry 24 hours of the vacuum drying oven of 755mmHg; Compound rest is put into the pore particle that the beaker that contains the 500ml deionized water leaches adhesive layer, changed water one time in per 4 hours, detect with silver nitrate solution after 48 hours, white precipitate does not appear in leachate, and it is clean to illustrate that particle has removed.Compound rest is taken out from water, the filter paper suction, air at room temperature changes the vacuum drying oven drying of vacuum greater than 755mmHg over to after dry 24 hours.Make by two PLGA porous supports and form the complex stephanoporate bracket that communicates with each other.Porosity 90%, aperture 350-455 μ m.
Embodiment 2
Support adopts two different porosities to get the column PLGA85/15 porous support of paraffin microsphere pore, porosity 95% wherein, another piece 85% (high 5mm, diameter 10mm, aperture 350-455 μ m), remaining condition is identical with embodiment 1, makes a double-layer porous support of being made up of the different porosities material.
Embodiment 3
Support adopts two column PLGA85/15 porous support (high 5mm that different pore technologies obtain, diameter 10mm, porosity 90%, aperture 350-455 μ m), wherein one by the pore of sodium chloride salt grain, and one by the pore of paraffin microsphere, and all the other conditions are identical with embodiment 1, make one deck regular spherical hole, the complex stephanoporate bracket in the irregular hole of another layer.
Embodiment 4
Prepare three column PLGA85/15 porous support (high 5mm with the pore of paraffin microsphere, diameter 10mm, porosity 90%, aperture 350-455 μ m), after first and second bonding,, the 3rd is bondingly got on again at second upper surface application of adhesive, all the other methods are identical with embodiment 1, make three layers of complex stephanoporate bracket.
Embodiment 5
Binder particles is changed the paraffin microsphere of making 350-455 μ m, column PLGA85/15 porous support (high 5mm with the pore of two paraffin microspheres, diameter 10mm, porosity 90%, aperture 350-455 μ m) press embodiment 1 mode bonding after, place in the apparatus,Soxhlet's, be solvent extraction 48 hours with pentane, took out the support air at room temperature dry 8 hours, vacuum drying oven vacuum was greater than 755mmHg drying at room temperature 48 hours.Obtaining adhesive layer is the two-layer compound porous support of spherical pore.
Use 0.3g molecular weight is 50,000 polycaprolactone (PCL), is dissolved in the 10ml acetone, and the salt particle 2.7g of 350-455 μ m particle diameter, mix homogeneously is made binding agent, and all the other methods are identical with embodiment 1, make the PLGA85/15 complex stephanoporate bracket that is connected by PCL.
Claims (18)
1. a multi-layer porous scaffold is characterized in that, described support has multiple structure, and each interlayer of support contains porous transition zone, is interconnected between the hole of support levels; Described transition zone makes by following method: adopt the mixture of being made up of polymer, solvent and pore particle as binding agent, remove particle after the curing, form the transition layer structure that is interconnected.
2. multi-layer porous scaffold according to claim 1 is characterized in that, one or more layers in the described multi-layer porous scaffold includes the loose structure of aperture 10-900 μ m, porosity 20%-99%, and the aperture of different layers is identical with porosity or inequality.
3. multi-layer porous scaffold according to claim 1 is characterized in that, the host material of forming each layer support comprises macromolecular material, inorganic material, bio-derived material or their complex; The host material of forming different layers is identical or inequality.
4. multi-layer porous scaffold according to claim 3 is characterized in that described host material is to have from cementability, resolvability or plastic natural or synthesized polymer material.
5. multi-layer porous scaffold according to claim 3 is characterized in that described macromolecular material is selected from the complex of degradable macromolecule or nondegradable macromolecular material or macromolecular material and bio-derived material or inorganic material.
6. multi-layer porous scaffold according to claim 5 is characterized in that described degradable macromolecular material is selected from the copolymer or the blend of polylactic acid, polyglycolic acid, poly butyric ester, polycaprolactone, Merlon, poe, poly-anhydride, poly-dioxane, chitosan, alginate, hyaluronate, collagen, gelatin or fibroin albumen or two or more composition in them.
7. multi-layer porous scaffold according to claim 5 is characterized in that described nondegradable macromolecular material is selected from polystyrene, polrvinyl chloride, polyacrylate, polymethacrylates, Merlon, nylon, polyurethane, polyformaldehyde, polyvinyl alcohol, polyvinyl acetate or polysiloxanes or by two or more copolymer formed or blend in them.
8. according to the described arbitrary multi-layer porous scaffold of claim 1-7, any one or a few preparation in particle hole forming technology, non-woven fabrics technology, foaming technology or phase detachment technique and the 3 D-printing technology is adopted in the molding that it is characterized in that described each layer of support, and wherein the thickness of each layer support is 0.1-100mm.
9. the preparation method of a multi-layer porous scaffold is characterized in that comprising the steps:
(1) will make the solvent of polymer raw material, polymer of support and pore particle and stir into the binding agent that obtains the multilamellar support behind the mixture, described solvent is a solvent orange 2 A;
(2) porous support that will make in advance respectively forms the direct use of shelf layer or cuts out the acquisition adhesive surface by required form;
(3) binding agent of step 1) is coated on the adhesive surface, behind the formation articulamentum, will treat placed on it of bonding another layer support, and exerts pressure, and treats to finalize the design after the solvent evaporates;
(4) after typing is finished, remove pressure, also need add shelf layer, repeat previous action;
(5) above-mentioned interconnective multilamellar support is implemented particle and remove, use the particle stripping of the solvent of particle with articulamentum, behind the dry removal residual solvent, the MULTILAYER COMPOSITE porous support that must have the pore structure of being interconnected, the solvent of described particle are solvent B.
10. according to the preparation method of claim 9, it is characterized in that the amount ranges of pore particle in the described binding agent accounts for the 20wt%-99wt% of mixture, the volume fraction of solvent is 20%-70%, and the thickness of adhesive layer is 0.01-5mm.
11., it is characterized in that the polymer in the described binding agent is selected from degradable polylactic acid, polyglycolic acid, poly butyric ester, polycaprolactone, Merlon, poe, poly-anhydride, poly-dioxane, chitosan, alginate, hyaluronate, collagen, gelatin, fibroin albumen according to the preparation method of claim 9; Or nondegradable polystyrene, polrvinyl chloride, polyacrylate, polymethacrylates, Merlon, nylon, polyurethane, polyformaldehyde, polyvinyl alcohol, polyvinyl acetate, polysiloxanes; Or a kind of by two or more copolymer formed in them or blend.
12. preparation method according to claim 9, it is characterized in that the solvent orange 2 A in the described binding agent is selected from acetone, butanone, chloroform, dichloromethane, oxolane, benzene,toluene,xylene, ethylene glycol, Ketohexamethylene, dioxane, N, dinethylformamide, formic acid, benzyl alcohol or cyclohexane extraction or one or more mixture wherein.
13., it is characterized in that the mixture that the pore particle in the described binding agent is selected from inorganic salt, polysaccharide, organic micromolecule compound or is made up of them according to the preparation method of claim 9; Described particle is the ball-type or the irregular polyhedron of rule, and particle size range is 10-900 μ m.
14., it is characterized in that described solvent B is selected from one or more the mixture in pentane, hexane, heptane, octane, cyclohexane extraction or the water according to the preparation method of claim 9.
15., it is characterized in that the amount ranges of pore particle in the described binding agent accounts for the 70wt%-95wt% of mixture according to the preparation method of claim 9.
16., it is characterized in that the shared volume fraction of solvent is 30%-50% in the described binding agent according to the preparation method of claim 9.
17. according to the preparation method of claim 9, it is characterized in that solvent has dissolubility to binder polymer component or backing substrate material in the described binding agent, do not change its structure and performance; The pore particle is not dissolved and do not change its structure and character; Under vacuum condition, remove fully.
18. the purposes of the multi-layer porous scaffold of claim 1 in the cytoskeleton of preparation reparation skin, bone or cartilage, blood vessel, esophagus or tracheal tissue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910050499 CN101874751B (en) | 2009-04-30 | 2009-04-30 | Multi-layer porous scaffold and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910050499 CN101874751B (en) | 2009-04-30 | 2009-04-30 | Multi-layer porous scaffold and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101874751A true CN101874751A (en) | 2010-11-03 |
| CN101874751B CN101874751B (en) | 2013-07-10 |
Family
ID=43017502
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910050499 Expired - Fee Related CN101874751B (en) | 2009-04-30 | 2009-04-30 | Multi-layer porous scaffold and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101874751B (en) |
Cited By (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102018993A (en) * | 2010-12-07 | 2011-04-20 | 天津大学 | Porous bracket with graded aperture distribution and manufacture method thereof |
| CN102321352A (en) * | 2011-03-18 | 2012-01-18 | 华东理工大学 | Polycaprolactone in mesoporous structure and preparation method and application thereof |
| CN102743796A (en) * | 2011-04-18 | 2012-10-24 | 北京汇亨创管理咨询有限公司 | Silk fibroin porous support made from polyvinyl alcohol, and preparation method and application thereof |
| CN103191470A (en) * | 2013-04-03 | 2013-07-10 | 中国科学院上海硅酸盐研究所 | Organic/inorganic composite three-dimensional porous scaffold with drug sustained release function, and preparation method thereof |
| CN103980683A (en) * | 2014-04-30 | 2014-08-13 | 中国科学院化学研究所 | Biodegradable polylactic acid material for 3D printing and preparation method thereof |
| CN104068945A (en) * | 2014-06-27 | 2014-10-01 | 深圳齐康医疗器械有限公司 | Artificial skin and preparation method thereof |
| CN105611952A (en) * | 2013-07-16 | 2016-05-25 | 岭南大学校产学协力团 | Method for preparing bilayer scaffold through single process and method for regenerating tissue using bilayer scaffold obtained by preparing method |
| CN105748179A (en) * | 2016-04-27 | 2016-07-13 | 常州大学 | Personalized airway stent manufacturing technology |
| CN106163581A (en) * | 2013-11-05 | 2016-11-23 | 哈佛学院院长及董事 | The method printing the tissue construct of the vascular system with embedding |
| CN106890359A (en) * | 2015-12-17 | 2017-06-27 | 四川大学 | The three-dimensional porous polyurethane support and preparation method repaired for central nervous system injury |
| KR101757397B1 (en) * | 2014-10-22 | 2017-07-13 | 한남대학교 산학협력단 | Porous matrix for trachea regeneration and method for preparing thereof |
| CN107233613A (en) * | 2017-06-07 | 2017-10-10 | 中国海洋大学 | A kind of aquatic origin cross-linked collagen composite multi-layer medical dressing |
| CN107669381A (en) * | 2017-11-29 | 2018-02-09 | 成都创客之家科技有限公司 | A kind of medicament-release blood vessel stent |
| CN107669380A (en) * | 2017-11-29 | 2018-02-09 | 成都创客之家科技有限公司 | A kind of drug eluting vascular support |
| CN107822740A (en) * | 2017-10-25 | 2018-03-23 | 中国人民解放军总医院 | Artery medicine elution bracket of biological absorbable material and preparation method thereof |
| CN107822751A (en) * | 2017-10-25 | 2018-03-23 | 中国人民解放军总医院 | Artery medicine elution bracket based on 3D printing technique and preparation method thereof |
| CN108159493A (en) * | 2017-12-28 | 2018-06-15 | 山东省日照市人民医院 | A kind of preparation method of alginate-hydrogel nano fiber scaffold |
| CN108201634A (en) * | 2016-12-20 | 2018-06-26 | 重庆润泽医药有限公司 | A kind of joint repair stent |
| CN108201632A (en) * | 2016-12-20 | 2018-06-26 | 重庆润泽医药有限公司 | A kind of articular cartilage repaiies scaffold |
| CN108434528A (en) * | 2018-04-17 | 2018-08-24 | 武汉理工大学 | A kind of intensifying method of chitosan electrostatic spinning composite nerve conduit |
| CN108498843A (en) * | 2018-06-13 | 2018-09-07 | 暨南大学 | A kind of 3 D-printing anti-bacterial hydrogel dressing and preparation method thereof |
| CN108912628A (en) * | 2018-05-31 | 2018-11-30 | 苏州乔纳森新材料科技有限公司 | A kind of intubation preparation method of antimicrobial nano material |
| CN109091705A (en) * | 2018-10-23 | 2018-12-28 | 吕洋 | A kind of three-dimensional porous rack and its preparation method and application |
| CN109096501A (en) * | 2018-07-16 | 2018-12-28 | 武汉纺织大学 | A kind of fibroin three-dimensional porous rack and preparation method thereof |
| CN109562201A (en) * | 2016-07-25 | 2019-04-02 | 宇部兴产株式会社 | For treating the implantation material of bone injury site and the treatment method of kit and bone injury site |
| CN109589456A (en) * | 2017-09-30 | 2019-04-09 | 先健科技(深圳)有限公司 | Implantable device |
| CN110944685A (en) * | 2017-05-10 | 2020-03-31 | 阿肯色大学理事会 | Biocompatible structures for tissue regeneration and methods of making and using the same |
| CN111501204A (en) * | 2020-04-03 | 2020-08-07 | 陈美荷 | Comfortable protective gloves and preparation method thereof |
| EP3799892A1 (en) | 2016-10-28 | 2021-04-07 | Dialybrid S.r.l. | Hybrid scaffold suitable for regenerating animal tissues and process for producing the scaffold |
| CN112979779A (en) * | 2019-11-29 | 2021-06-18 | 苏州丝美特生物技术有限公司 | Method for processing silk fibroin by aromatic alcohol and application of silk fibroin-containing material |
| CN113144286A (en) * | 2021-04-21 | 2021-07-23 | 四川大学华西医院 | Degradable self-supporting artificial bile duct and preparation method thereof |
| CN113185793A (en) * | 2021-06-01 | 2021-07-30 | 阮媛媛 | Modified polyvinyl alcohol used as supporting material, preparation method and removal method |
| CN113349988A (en) * | 2021-05-31 | 2021-09-07 | 浙江大学 | Tissue engineering bone for repairing jaw cleft palate defect and preparation method thereof |
| CN113941030A (en) * | 2021-10-27 | 2022-01-18 | 西安交通大学 | Vascular tissue engineering scaffold with ultrasonic-assisted 3D printing and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1386478A (en) * | 2001-05-23 | 2002-12-25 | 中国科学院化学研究所 | Cell scaffold with composite structure for tissue engineering and its preparing process and application |
| CN1943801A (en) * | 2006-11-01 | 2007-04-11 | 华中科技大学 | A gradient laminated composite supporting frame material based on bionic structures and its preparation method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1319605C (en) * | 2005-12-07 | 2007-06-06 | 浙江大学 | Collagen-chitin and silicon rubber bilayer skin regeneration support and its preparation method |
| CN100558414C (en) * | 2007-05-25 | 2009-11-11 | 浙江大学 | A kind of cartilage tissue engineering rack and application thereof |
| CN101219069A (en) * | 2008-01-25 | 2008-07-16 | 中国人民武装警察部队医学院附属医院 | Double-layer composite bracket for renovating cartilage |
| CN101254313B (en) * | 2008-04-03 | 2011-05-11 | 厦门大学 | Double-layer collagen-chitosan sponges bracket and method of preparing the same |
-
2009
- 2009-04-30 CN CN 200910050499 patent/CN101874751B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1386478A (en) * | 2001-05-23 | 2002-12-25 | 中国科学院化学研究所 | Cell scaffold with composite structure for tissue engineering and its preparing process and application |
| CN1943801A (en) * | 2006-11-01 | 2007-04-11 | 华中科技大学 | A gradient laminated composite supporting frame material based on bionic structures and its preparation method |
Cited By (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102018993B (en) * | 2010-12-07 | 2013-08-14 | 天津大学 | Porous bracket with graded aperture distribution and manufacture method thereof |
| CN102018993A (en) * | 2010-12-07 | 2011-04-20 | 天津大学 | Porous bracket with graded aperture distribution and manufacture method thereof |
| CN102321352A (en) * | 2011-03-18 | 2012-01-18 | 华东理工大学 | Polycaprolactone in mesoporous structure and preparation method and application thereof |
| CN102743796A (en) * | 2011-04-18 | 2012-10-24 | 北京汇亨创管理咨询有限公司 | Silk fibroin porous support made from polyvinyl alcohol, and preparation method and application thereof |
| CN103191470A (en) * | 2013-04-03 | 2013-07-10 | 中国科学院上海硅酸盐研究所 | Organic/inorganic composite three-dimensional porous scaffold with drug sustained release function, and preparation method thereof |
| CN103191470B (en) * | 2013-04-03 | 2014-11-26 | 中国科学院上海硅酸盐研究所 | Organic/inorganic composite three-dimensional porous scaffold with drug sustained release function, and preparation method thereof |
| CN105611952A (en) * | 2013-07-16 | 2016-05-25 | 岭南大学校产学协力团 | Method for preparing bilayer scaffold through single process and method for regenerating tissue using bilayer scaffold obtained by preparing method |
| CN105611952B (en) * | 2013-07-16 | 2018-05-29 | 岭南大学校产学协力团 | The method that the method for double-layer scaffold is prepared by single step process and regeneration is carried out using the double-layer scaffold obtained by the preparation method |
| CN106163581B (en) * | 2013-11-05 | 2019-10-25 | 哈佛学院院长及董事 | Print the method with the tissue construct of vascular system of embedding |
| CN106163581A (en) * | 2013-11-05 | 2016-11-23 | 哈佛学院院长及董事 | The method printing the tissue construct of the vascular system with embedding |
| CN103980683A (en) * | 2014-04-30 | 2014-08-13 | 中国科学院化学研究所 | Biodegradable polylactic acid material for 3D printing and preparation method thereof |
| CN103980683B (en) * | 2014-04-30 | 2017-06-16 | 中国科学院化学研究所 | A kind of 3 D-printing biodegradable polylactic acid material and preparation method thereof |
| CN104068945A (en) * | 2014-06-27 | 2014-10-01 | 深圳齐康医疗器械有限公司 | Artificial skin and preparation method thereof |
| CN104068945B (en) * | 2014-06-27 | 2016-11-16 | 深圳齐康医疗器械有限公司 | A kind of artificial skin and preparation method thereof |
| KR101757397B1 (en) * | 2014-10-22 | 2017-07-13 | 한남대학교 산학협력단 | Porous matrix for trachea regeneration and method for preparing thereof |
| CN106890359A (en) * | 2015-12-17 | 2017-06-27 | 四川大学 | The three-dimensional porous polyurethane support and preparation method repaired for central nervous system injury |
| CN105748179A (en) * | 2016-04-27 | 2016-07-13 | 常州大学 | Personalized airway stent manufacturing technology |
| CN109562201A (en) * | 2016-07-25 | 2019-04-02 | 宇部兴产株式会社 | For treating the implantation material of bone injury site and the treatment method of kit and bone injury site |
| EP3799892A1 (en) | 2016-10-28 | 2021-04-07 | Dialybrid S.r.l. | Hybrid scaffold suitable for regenerating animal tissues and process for producing the scaffold |
| US11213612B2 (en) | 2016-10-28 | 2022-01-04 | Dialybrid S.r.l. | Hybrid scaffold suitable for regenerating animal tissues and process for producing the scaffold |
| CN108201634B (en) * | 2016-12-20 | 2020-09-29 | 重庆润泽医药有限公司 | Bracket for joint repair |
| CN108201634A (en) * | 2016-12-20 | 2018-06-26 | 重庆润泽医药有限公司 | A kind of joint repair stent |
| CN108201632A (en) * | 2016-12-20 | 2018-06-26 | 重庆润泽医药有限公司 | A kind of articular cartilage repaiies scaffold |
| CN110944685A (en) * | 2017-05-10 | 2020-03-31 | 阿肯色大学理事会 | Biocompatible structures for tissue regeneration and methods of making and using the same |
| CN107233613A (en) * | 2017-06-07 | 2017-10-10 | 中国海洋大学 | A kind of aquatic origin cross-linked collagen composite multi-layer medical dressing |
| CN109589456B (en) * | 2017-09-30 | 2024-03-19 | 元心科技(深圳)有限公司 | Implantable device |
| CN109589456A (en) * | 2017-09-30 | 2019-04-09 | 先健科技(深圳)有限公司 | Implantable device |
| CN107822751A (en) * | 2017-10-25 | 2018-03-23 | 中国人民解放军总医院 | Artery medicine elution bracket based on 3D printing technique and preparation method thereof |
| CN107822740A (en) * | 2017-10-25 | 2018-03-23 | 中国人民解放军总医院 | Artery medicine elution bracket of biological absorbable material and preparation method thereof |
| CN107669380A (en) * | 2017-11-29 | 2018-02-09 | 成都创客之家科技有限公司 | A kind of drug eluting vascular support |
| CN107669381A (en) * | 2017-11-29 | 2018-02-09 | 成都创客之家科技有限公司 | A kind of medicament-release blood vessel stent |
| CN108159493B (en) * | 2017-12-28 | 2020-11-20 | 山东省日照市人民医院 | Preparation method of alginate-hydrogel nanofiber scaffold |
| CN108159493A (en) * | 2017-12-28 | 2018-06-15 | 山东省日照市人民医院 | A kind of preparation method of alginate-hydrogel nano fiber scaffold |
| CN108434528B (en) * | 2018-04-17 | 2020-12-22 | 武汉理工大学 | Method for strengthening chitosan electrostatic spinning composite nerve conduit |
| CN108434528A (en) * | 2018-04-17 | 2018-08-24 | 武汉理工大学 | A kind of intensifying method of chitosan electrostatic spinning composite nerve conduit |
| CN108912628A (en) * | 2018-05-31 | 2018-11-30 | 苏州乔纳森新材料科技有限公司 | A kind of intubation preparation method of antimicrobial nano material |
| CN108498843A (en) * | 2018-06-13 | 2018-09-07 | 暨南大学 | A kind of 3 D-printing anti-bacterial hydrogel dressing and preparation method thereof |
| CN108498843B (en) * | 2018-06-13 | 2021-06-11 | 暨南大学 | Three-dimensional printing antibacterial hydrogel dressing and preparation method thereof |
| CN109096501A (en) * | 2018-07-16 | 2018-12-28 | 武汉纺织大学 | A kind of fibroin three-dimensional porous rack and preparation method thereof |
| CN109091705A (en) * | 2018-10-23 | 2018-12-28 | 吕洋 | A kind of three-dimensional porous rack and its preparation method and application |
| CN109091705B (en) * | 2018-10-23 | 2019-08-06 | 吕洋 | A kind of three-dimensional porous rack and its preparation method and application |
| CN112979779B (en) * | 2019-11-29 | 2024-02-09 | 苏州丝美特生物技术有限公司 | Method for treating silk fibroin by aromatic alcohol and application of silk fibroin in preparation of silk fibroin-containing material |
| CN112979779A (en) * | 2019-11-29 | 2021-06-18 | 苏州丝美特生物技术有限公司 | Method for processing silk fibroin by aromatic alcohol and application of silk fibroin-containing material |
| CN111501204A (en) * | 2020-04-03 | 2020-08-07 | 陈美荷 | Comfortable protective gloves and preparation method thereof |
| CN113144286A (en) * | 2021-04-21 | 2021-07-23 | 四川大学华西医院 | Degradable self-supporting artificial bile duct and preparation method thereof |
| CN113349988A (en) * | 2021-05-31 | 2021-09-07 | 浙江大学 | Tissue engineering bone for repairing jaw cleft palate defect and preparation method thereof |
| CN113185793A (en) * | 2021-06-01 | 2021-07-30 | 阮媛媛 | Modified polyvinyl alcohol used as supporting material, preparation method and removal method |
| CN113185793B (en) * | 2021-06-01 | 2022-12-27 | 浙江柏明胜医疗科技有限公司 | Modified polyvinyl alcohol used as supporting material, preparation method and removal method |
| CN113941030A (en) * | 2021-10-27 | 2022-01-18 | 西安交通大学 | Vascular tissue engineering scaffold with ultrasonic-assisted 3D printing and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101874751B (en) | 2013-07-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101874751B (en) | Multi-layer porous scaffold and preparation method thereof | |
| Khandan et al. | Hydrogels: Types, structure, properties, and applications | |
| Pandey et al. | Chitosan: Application in tissue engineering and skin grafting | |
| Feng et al. | Structural and functional adaptive artificial bone: materials, fabrications, and properties | |
| Khorshidi et al. | A review of key challenges of electrospun scaffolds for tissue‐engineering applications | |
| Annabi et al. | Controlling the porosity and microarchitecture of hydrogels for tissue engineering | |
| Walker et al. | Processing and production of bioresorbable polymer scaffolds for tissue engineering | |
| JP5049119B2 (en) | Biocompatible bone implant composition and method for repairing bone defects | |
| Zhang et al. | Silk fibroin microfibers and chitosan modified poly (glycerol sebacate) composite scaffolds for skin tissue engineering | |
| Zhang et al. | A review of preparation methods of porous skin tissue engineering scaffolds | |
| Guarino et al. | Temperature-driven processing techniques for manufacturing fully interconnected porous scaffolds in bone tissue engineering | |
| KR100621569B1 (en) | Nano-microfibrous scaffold for enhanced tissue regeneration and method for preparing the same | |
| CN101920043B (en) | Porous bracket with micro grooves on pore walls and preparation method thereof | |
| CN102178980A (en) | Natural polymer composite porous fibrous scaffold and preparation method thereof | |
| CN101176799A (en) | Method for preparing polyalcohol stephanoporate bracket for tissue project by poragen agglutinating filtering off method | |
| Karande et al. | Function and requirement of synthetic scaffolds in tissue engineering | |
| Tamay et al. | Bioinks—materials used in printing cells in designed 3D forms | |
| CN109364288B (en) | Application of hole-hole composite micro-nano structure polysaccharide microspheres in preparation of hemostatic dressing | |
| Parisi et al. | Colonization versus encapsulation in cell-laden materials design: porosity and process biocompatibility determine cellularization pathways | |
| CN1238063C (en) | Porous rack with spherical pores and its molding prepn process | |
| CN102432911B (en) | Particle with surface topological topography, porous bracket and preparation method thereof | |
| CN109517225B (en) | Hole-hole composite micro-nano structure polysaccharide microsphere and preparation method thereof | |
| CN1438041A (en) | Biological active bone tissue inducing regeneration film and preparation method | |
| WO2017116355A1 (en) | Tissue scaffold with enhanced biocompatibility and mechanical properties and a method for producing it | |
| Pina et al. | Biomimetic strategies to engineer mineralised human tissues |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130710 Termination date: 20180430 |