CN101861860A - Squalene plant fungicide and uses thereof - Google Patents
Squalene plant fungicide and uses thereof Download PDFInfo
- Publication number
- CN101861860A CN101861860A CN201010212511A CN201010212511A CN101861860A CN 101861860 A CN101861860 A CN 101861860A CN 201010212511 A CN201010212511 A CN 201010212511A CN 201010212511 A CN201010212511 A CN 201010212511A CN 101861860 A CN101861860 A CN 101861860A
- Authority
- CN
- China
- Prior art keywords
- squalene
- fungicide
- emulsifier
- weight portion
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a squalene plant fungicide, which is prepared by using squalene as a main raw material and then adding a solvent, a solubilizer and/or an emulsifier, is an environmental friendly harmonious pesticide which is safe for human and livestock, and has profound significance for long-term development of ecological environment, human health and comprehensive control of diseases. Compared with the traditional pesticide, the squalene plant fungicide has the following characteristics and advantages that: 1, squalene has high biological activity; 2, the squalene widely exists in plants and animals, has various sources and can become good development resources; 3, because of coming from the nature, the squalene is difficult to cause pesticide injury and toxic residues, and is safe for human and livestock and ecological enviroment; 4, the squalene is easily synthesized, has wide natural sources and provides convenient conditions for industrial production; and 5, the squalene is combined with other effective sterilization ingredients of preparative plants to be mixed to prepare the fungicide so as to further enhance the bactericidal effect on a farm orchard.
Description
Technical field
The present invention relates to the new purposes of a kind of squalene plant fungicide and squalene.
Background technology
Squalene has another name called shark alkene, squalene, dog-fish oil element or cod-liver oil terpene etc., chemical formula C
30H
50, chemical name 2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosa carbon six alkene.Be colourless oil liquid, have pleasant smell, oxygen uptake becomes sticky as flax oily.Relative density 0.8584, fusing point-75 ℃, the about 330 ℃/normal pressure of boiling point (decomposition), 280 ℃/2.27kPa, 285 ℃/3.3kPa, 240～242 ℃/533Pa, 110 ℃ of flash-points, index of refraction 1.4965 (20 ℃), viscosity 0.012Pa*s is dissolved in ether, benzinum, acetone and carbon tetrachloride, be slightly soluble in pure and mild glacial acetic acid, water insoluble.Squalene does not exist only in the animal body, the part such as root, leaf, skin that also is present in many plants, in Salvia japonica, tobacco leaf, Abies sibirica bark, pteridophyte, green algae and bryophyte, content is also than horn of plenty in olive oil and the palm oil.Because of it has stronger activity and oxygen carrying capacity, be widely used in aspects such as healthcare products and cosmetics at present.But still there be not the application report of squalene in the preparation botanical fungicide in the existing document; Still do not have at present a kind of good with environment compatibility, to the harmonious agricultural chemicals of mammalian safe.
Summary of the invention
The object of the invention is to disclose a kind of squalene plant fungicide, and order of the present invention also is the new purposes of open squalene in the preparation plant fungicide.
The present invention seeks to be achieved through the following technical solutions:
The application of squalene in the preparation plant fungicide.
Squalene is added conventional auxiliary material, according to common process, make various forms of fungicides: missible oil, suspending agent, pulvis or spray.
Wherein, auxiliary material is a solvent, can also add solubilizer and/or emulsifier.Solvent is acetone, chloroform, benzene and/or dimethylbenzene, preferred dimethylbenzene; Solubilizer, emulsifier are spans, Tweens, glycol ester class, propylene glycol ester class and/or polyethylene glycol type non-ionic surface active agent.
Wherein, the raw material of missible oil consists of in the squalene fungicide of the present invention:
Squalene 20-60 weight portion dimethylbenzene 30-70 weight portion emulsifier 5-15 weight portion.
The raw material of missible oil composition is preferably in the squalene fungicide of the present invention:
Squalene 50 weight portion dimethylbenzene 40 weight portion emulsifier 10 weight portions.
The raw material of missible oil composition is preferably in the squalene fungicide of the present invention:
Squalene 30 weight portion dimethylbenzene 60 weight portion emulsifier 10 weight portions.
The raw material of missible oil composition is preferably in the squalene fungicide of the present invention:
Squalene 40 weight portion dimethylbenzene 50 weight portion emulsifier 10 weight portions.
Wherein, emulsifier is that the HLB value is 15 Tween-80.
The preparation method of missible oil is in the squalene fungicide of the present invention: with the squalene xylene soluble, add emulsifier therein again, dissolving promptly can be used for sterilization.
Wherein, described fungi can be a Monilinia fructicola.
The invention provides a kind ofly, the comprehensive regulation of ecotope long term growth, human beings'health and disease is all had profound significance person poultry safety, environmentally friendly harmonious agricultural chemicals.Squalene plant fungicide of the present invention is compared with traditional agricultural chemicals, have following characteristics and advantage: 1, squalene has higher biologically active, through the spore germination determination experiment, can be when concentration be 2000 μ g/ml, the spore of the brown germ of peach is suppressed by squalene fully.Squalene with acetone diluted after, through suppressing the mycelial growth test, can be when concentration be 2000g/ml, squalene can reach 92.64% to the bacteriostasis rate of the brown germ of peach, visible squalene has stronger pesticide developing and is worth.2, squalene extensively is formed in the bodies of aminal and plant, and wide material sources can become well and exploit natural resources; 3, squalene derives from nature because of it, is difficult for causing pesticide injury, toxic residue, to people and animals, ecological environment security; 4, it is extensive to carry out synthetic and natural source easily, for carrying out the condition that suitability for industrialized production is provided convenience; 5, in conjunction with other effective sterilization components in the preparation type plant, pharmaceutical mixture can further strengthen the bactericidal action on the orchard, farmland; 6, missible oil is solvent with dimethylbenzene in the squalene plant fungicide of the present invention, can increase the solvability of squalene in water, and has the effect that promotes agricultural chemicals infiltration plant epidermis concurrently.
Description of drawings
Fig. 1: squalene is to the influence of peach brown rot fungus spore germination;
Fig. 2: the Monilinia fructicola that squalene is handled under the ESEM and the spore and the mycelia form of contrast;
Fig. 3: squalene is handled spore structure and the contrast of Monilinia fructicola under the transmission electron microscope.
Following experimental example and embodiment are used for further specifying but are not limited to the present invention.
The screening experiment of experimental example 1 solvent of the present invention.
This experiment is screened the solvent of squalene, adopt respectively acetone, chloroform, benzene, dimethylbenzene as the solvent of squalene, adopt growth rate method, Monilinia fructicola is carried out BA, concentration is made as 1500 μ g/ml, add a Tween-80 and make emulsifying agent, the results are shown in Table 1, optimize and select dimethylbenzene as optimum solvent.
Squalene is to the inhibitory action of Monilinia fructicola in table 1 different solvents
Experimental example 2 squalene plant fungicides of the present invention are to the inhibition experiment of Monilinia fructicola.
Prepare the antifungal missible oil of squalene plant of the present invention according to the embodiment of the invention 1 described raw material and method, this missible oil suppresses experiment through water dilution (extension rate sees Table 2) to Monilinia fructicola. Experimental result sees Table 3:
The table 2 concentration dilution multiple table of comparisons
The antifungal missible oil of table 3 squalene plant of the present invention suppresses the result to the biologically active of Monilinia fructicola
Annotate: lowercase alphabet shows that the F value less than 0.05, has significant difference among the figure.
Experimental example 3 squalenes are to the fungus spore germination determination experiment
With acetone squalene is diluted to variable concentrations, measures it to the sprouting situation of peach brown rot fungus spore, result of the test is seen Fig. 1, and when concentration was 2000 μ g/ml, the spore of the brown germ of peach was suppressed by squalene fully.
Continuation has been carried out further research to the antifungal mechanism of squalene, processing the peach brown rot fungus with squalene is processed group, process the peach brown rot fungus take the end through squalene and be control group, ESEM is also observed, and it is coarse and uneven to found that the Monilinia fructicola spore surface becomes; Mycelium also be distorted, rupture (such as accompanying drawing 2). Transmission electron microscope observing finds that cavity appears in the organelle degraded of peach brown rot bacterial cell, and kytoplasm aggegation, appearance are run off, and eucaryotic cell structure is destroyed, and causes cell death (such as accompanying drawing 3). Electron microscopic observation result further checking squalene of the present invention has significant inhibitory action to Monilinia fructicola.
Following embodiment all can realize the described effect of above-mentioned experimental example.
Embodiment
Embodiment 1: squalene plant antifungal missible oil of the present invention
Squalene 50kg dimethylbenzene 40kg HLB value is 15 Tween-80 10kg.
With the squalene xylene soluble, add the HLB value therein again and be 15 Tween-80, dissolving can be adopted spray pattern control Monilinia fructicola.
Embodiment 2: squalene plant antifungal missible oil of the present invention
Squalene 30kg dimethylbenzene 60kg HLB value is 15 Tween-80 10kg.
With the squalene xylene soluble, add emulsifier therein again, dissolving can be adopted spray pattern control Monilinia fructicola.
Embodiment 3: squalene plant antifungal missible oil of the present invention
Squalene 40kg dimethylbenzene 50kg HLB value is 15 Tween-80 10kg.
With the squalene xylene soluble, add emulsifier therein again, dissolving can be adopted spray pattern control Monilinia fructicola.
Embodiment 4: squalene plant antifungal suspending agent of the present invention
Squalene added in the solvent acetone dissolve, add Arlacel-20 again, make squalene plant antifungal suspending agent, adopt the spraying mode to prevent and treat plant epiphyte according to common process.
Embodiment 5: squalene plant antifungal pulvis of the present invention
According to common process, add conventional auxiliary material, squalene is made pulvis, adopt the spraying mode to prevent and treat plant epiphyte.
Embodiment 6: squalene plant antifungal spray of the present invention
Squalene added in the solvent chloroform dissolve, add ethylene glycol again, make squalene plant antifungal spray, adopt the spraying mode to prevent and treat plant epiphyte according to common process.
Claims (10)
1. squalene plant fungicide is characterized in that the raw material of this fungicide consists of:
Squalene 20-60 weight portion dimethylbenzene 30-70 weight portion emulsifier 5-15 weight portion.
2. fungicide as claimed in claim 1 is characterized in that the raw material of this fungicide consists of:
Squalene 50 weight portion dimethylbenzene 40 weight portion emulsifier 10 weight portions;
Or
Squalene 30 weight portion dimethylbenzene 60 weight portion emulsifier 10 weight portions;
Or
Squalene 40 weight portion dimethylbenzene 50 weight portion emulsifier 10 weight portions.
3. fungicide as claimed in claim 1 or 2 is characterized in that wherein said emulsifier is that the HLB value is 15 Tween-80.
4. as the preparation method of the arbitrary described fungicide of claim 1-3, it is characterized in that this method is:
With the squalene xylene soluble, add emulsifier therein again, dissolving promptly can be used for sterilization.
5. as the application of the arbitrary described fungicide of claim 1-3 in preparation control plant epiphyte medicine.
6. the application of squalene in the preparation plant fungicide.
7. as claim 5 or 6 described application, it is characterized in that wherein said plant epiphyte is a Monilinia fructicola.
8. as claim 6 or 7 described application, it is characterized in that when the preparation plant fungicide, squalene being added conventional auxiliary material, according to common process, make various forms of fungicides: missible oil, suspending agent, pulvis or spray.
9. application as claimed in claim 8 is characterized in that wherein said auxiliary material is a solvent: acetone, chloroform, benzene and/or dimethylbenzene.
10. application as claimed in claim 9 is characterized in that wherein said auxiliary material also adds solubilizer and/or emulsifier; Solvent is that solubilizer, emulsifier are spans, Tweens, glycol ester class, propylene glycol ester class and/or polyethylene glycol type non-ionic surface active agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010212511 CN101861860B (en) | 2010-06-22 | 2010-06-22 | Squalene plant fungicide and uses thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010212511 CN101861860B (en) | 2010-06-22 | 2010-06-22 | Squalene plant fungicide and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101861860A true CN101861860A (en) | 2010-10-20 |
| CN101861860B CN101861860B (en) | 2013-07-10 |
Family
ID=42953960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010212511 Expired - Fee Related CN101861860B (en) | 2010-06-22 | 2010-06-22 | Squalene plant fungicide and uses thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101861860B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869103A (en) * | 2010-06-22 | 2010-10-27 | 北京农学院 | Plant miticide of squalene and applications thereof |
| CN103798362A (en) * | 2014-03-11 | 2014-05-21 | 华中农业大学 | Application of terbinafine hydrochloride to preservation and fresh-keeping of picked citrus |
| CN114395401A (en) * | 2021-12-24 | 2022-04-26 | 神究富硒农业发展(山东)有限公司 | Cell repairing liquid, preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5484599A (en) * | 1993-11-30 | 1996-01-16 | The President And Fellows Of Harvard College | Control of insect pests |
| JP2006008573A (en) * | 2004-06-25 | 2006-01-12 | Katayama Chem Works Co Ltd | Wood preservative and wood processing method using the same |
| US20080146444A1 (en) * | 2006-12-19 | 2008-06-19 | Polymer Ventures, Inc. | Method and compositions for improving plant growth |
| CN101869103A (en) * | 2010-06-22 | 2010-10-27 | 北京农学院 | Plant miticide of squalene and applications thereof |
-
2010
- 2010-06-22 CN CN 201010212511 patent/CN101861860B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5484599A (en) * | 1993-11-30 | 1996-01-16 | The President And Fellows Of Harvard College | Control of insect pests |
| JP2006008573A (en) * | 2004-06-25 | 2006-01-12 | Katayama Chem Works Co Ltd | Wood preservative and wood processing method using the same |
| US20080146444A1 (en) * | 2006-12-19 | 2008-06-19 | Polymer Ventures, Inc. | Method and compositions for improving plant growth |
| CN101869103A (en) * | 2010-06-22 | 2010-10-27 | 北京农学院 | Plant miticide of squalene and applications thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869103A (en) * | 2010-06-22 | 2010-10-27 | 北京农学院 | Plant miticide of squalene and applications thereof |
| CN101869103B (en) * | 2010-06-22 | 2013-07-10 | 北京农学院 | Plant miticide of squalene and applications thereof |
| CN103798362A (en) * | 2014-03-11 | 2014-05-21 | 华中农业大学 | Application of terbinafine hydrochloride to preservation and fresh-keeping of picked citrus |
| CN114395401A (en) * | 2021-12-24 | 2022-04-26 | 神究富硒农业发展(山东)有限公司 | Cell repairing liquid, preparation method and application thereof |
| CN114395401B (en) * | 2021-12-24 | 2023-11-24 | 神究富硒农业发展(山东)有限公司 | Cell repair liquid, preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101861860B (en) | 2013-07-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107410294A (en) | A kind of biological pesticide used additives | |
| CN110476963A (en) | A kind of tank-mix adjuvants and its preparation method and application | |
| CN104322572A (en) | Botanical insecticide | |
| CN105532735A (en) | Bactericidal and insecticidal composition containing Bacillus amyloliquefaciens and matrine | |
| CN105123699B (en) | A kind of insecticide and acaricide containing thymol and Nylar | |
| CN105145663B (en) | A kind of plant pesticide composition containing nimbin and eucalyptus oil | |
| CN101861860B (en) | Squalene plant fungicide and uses thereof | |
| CN103907612A (en) | Pyriminostrobin-containing insecticidal composition | |
| CN101869103B (en) | Plant miticide of squalene and applications thereof | |
| CN103688961A (en) | A pesticide composition containing fluoropyridine furanone | |
| CN103563963B (en) | Insecticidal composition containing propylene glycol alginate | |
| CN103651403B (en) | A kind of Efficient insecticidal composition containing Spiromesifen | |
| CN101176472B (en) | Biological source agricultural bactericide and uses thereof | |
| CN101869104A (en) | Methyl oleate plant acaricide and application thereof | |
| CN101669495A (en) | Bactericidal composition containing kresoxim-methyl phenylate | |
| CN103155951A (en) | Insectofungicide composition of agricultural mineral oil and copper naphthenate | |
| CN100464636C (en) | Aqueous emulsion of pesticides Bassa, carbosulfan, phosphor methylpyrimidine, and preparation method | |
| CN103651367A (en) | Efficient insecticidal composition containing spiromesifen | |
| CN103651363A (en) | Pesticidal composition containing spiromesifen and nereistoxin | |
| CN103651548B (en) | A kind of Pesticidal combination containing Spiromesifen | |
| KR101963576B1 (en) | Botanical insecticide composition containing essential oil from wood extract and pine resin, and the preparation method thereof | |
| CN103621548B (en) | A kind of Pesticidal combination containing Spiromesifen and pyrethroid | |
| KR20050058782A (en) | Environment-frendly agent having excellent fungicidal and insecticidal activity and method for preparing the same | |
| CN105123788A (en) | Bactericide, preparing method of bactericide, preparation and application | |
| CN109418284A (en) | A kind of Pesticidal combination containing Biphenthrin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130710 Termination date: 20160622 |