CN101859022A - Slide and forming method thereof - Google Patents
Slide and forming method thereof Download PDFInfo
- Publication number
- CN101859022A CN101859022A CN200910049072A CN200910049072A CN101859022A CN 101859022 A CN101859022 A CN 101859022A CN 200910049072 A CN200910049072 A CN 200910049072A CN 200910049072 A CN200910049072 A CN 200910049072A CN 101859022 A CN101859022 A CN 101859022A
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- slide
- substrate
- metal
- metal pattern
- formation method
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Abstract
The invention relates to a slide which comprises a substrate and a metal pattern arranged on the substrate. The invention can obtain the advantages of high yield and low cost and can reduce the distance between an object to be tested and a grid line to ensure that the object to be tested and the grid line simultaneously fall into a field depth range. The invention also discloses a forming method of the slide.
Description
Technical field
The present invention is relevant for a kind of slide, particularly a kind of slide that comprises metal pattern.
Background technology
Please refer to Fig. 1, Fig. 1 is for showing the sectional view of known biological counting slide group, known biological counting slide group is to utilize high precision to grind the mould mode to form, groove 61 degree of depth of the microslide 6 of wherein biological counting slide group can reach about 10 microns precision, and the weight of cover glass 7 utilizations own is depressed, unnecessary biological specimen is expelled to outside the groove 61, to avoid bulking effect.The height in microslide 6 and cover glass 7 formed detection spaces is 5 to 10 microns, so that the determinand in the biological specimen (for example, sperm, blood cell, ovum cell) still can move about after compression to reach the purpose of accurate Calculation.Above-mentioned biological counting slide group because to the slide group to grind the mould accuracy requirement very high, therefore prices are rather stiff for biological counting slide group.
In addition, when microslide 6 and cover glass 7 formed detection spaces are big, because determinand is for being suspended in the biological specimen, therefore determinand is far away with the mesh lines distance that is arranged at cover glass 7, thereby can't fall into simultaneously in the microscopical field depth, both can't be simultaneously be situation clearly to cause mesh lines or determinand.
Therefore, as how reducing the cost of manufacture of biological counting slide group, and reducing the distance of mesh lines and determinand among the biological counting slide group, is the target of needing effort at present badly.
Summary of the invention
At the problems referred to above, one of the object of the invention provides a kind of slide and forming method thereof, it can reach high yield and have advantage cheaply, and can reduce between determinand and the mesh lines distance so that determinand and mesh lines fall into simultaneously in the microscopical field depth.
For achieving the above object, the slide of one embodiment of the invention comprises a kind of slide, comprises that a substrate and is arranged at the metal pattern of substrate.
For achieving the above object, the slide formation method of another embodiment of the present invention comprises a kind of slide formation method, comprises a substrate is provided; And a metal pattern is set in substrate, wherein metal pattern system forms by the combination of vacuum evaporation, sputter, plating or aforesaid way.
Below illustrate in detail by the specific embodiment conjunction with figs., when the effect that is easier to understand purpose of the present invention, technology contents, characteristics and is reached.
Description of drawings
Fig. 1 is for showing the sectional view of known biological counting slide group.
Fig. 2 is the side view according to the slide group of an embodiment of the present invention.
Fig. 3 is the vertical view according to first slide of an embodiment of the present invention.
Fig. 4 is the vertical view according to second slide of an embodiment of the present invention.
The primary clustering symbol description
1 first slide
11 first substrates
12 metal grid lines
13 corpse or other object for laboratory examination and chemical testing calculate the district
14 location circumference
2 second slides
21 second substrates
22 metal barricades
23 openings
6 microslides
61 grooves
7 cover glasses
Embodiment
Please refer to Fig. 2 and Fig. 3, wherein Fig. 2 is the side view according to the slide group of an embodiment of the present invention; Fig. 3 is the vertical view according to first slide 1 of an embodiment of the present invention.The slide of an embodiment of the present invention comprises that first substrate 11 and many strip metals mesh lines 12 are arranged at first substrate 11 to form first slide 1, and wherein metal grid lines 12 defines a plurality of corpse or other object for laboratory examination and chemical testing and calculates district 13.
It should be noted that metal grid lines 12 can form by the combination of vacuum evaporation, sputter, plating or aforesaid way.In detail, vacuum evaporation is in making the metallic atom evaporation among the vacuum state and form film on first substrate 11; Sputter can utilize argon ion bombardment target (target material), and the target atom that is hit changes into gas phase and analyses and is plated on first substrate 11; And plating can make metal separate out to be plated on first substrate 11 in negative electrode.
In addition as mentioned above, metal grid lines 12 also can make up by the mode more than any two of vacuum evaporation, sputter, plating mode form.In detail, among an embodiment, metal grid lines 12 is to form a film by vacuum evaporation or sputtering way on first substrate 11, utilizes plating mode that film is increased to a predetermined altitude again.
As shown in Figure 3, among this embodiment, calculating district 13 by the defined corpse or other object for laboratory examination and chemical testing of metal grid lines 12 be rectangle, that is be vertically mutual between representative level and the vertical metal grid lines 12.
In one embodiment, above-mentioned metal grid lines 12 has a predetermined altitude, and its scope can be 0.3, and how rice is to 100 microns.Above-mentioned predetermined altitude can accurately be controlled, with the activity space of restriction determinand (for example, sperm, blood cell, ovum cell).
Slide of the present invention can comprise a setting circle week 14 again, and this location circumference 14 can be in order to assist the position of location determinand.Wherein, location circumference 14 can form by the combination of vacuum evaporation, sputter, plating or aforesaid way.
In one embodiment, first substrate 11 is the transparent use that is beneficial to optical microscope, and its material can be glass or acryl.
Please refer to Fig. 2 and Fig. 4, wherein Fig. 4 is the vertical view according to second slide 2 of an embodiment of the present invention.As shown in the figure, the slide of another embodiment of the present invention comprises second substrate 21; And a metal barricade 22 is set to define a recessed space on second substrate 21, therefore form second slide 2.
Wherein metal barricade 22 can being combined to form by vacuum evaporation, sputter, plating or aforesaid way.Recessed space can be in order to the carrying biological specimen carrying out subsequent analysis, and metal barricade 22 has at least one opening 23, with so that the unnecessary biological specimen that splashes in recessed space flow out.Wherein, the formation of metal barricade 22 is identical with above-mentioned metal grid lines 12 generation types, and the material of second substrate 21 also can be identical with first substrate 11, is not described in detail in this.
In one embodiment, above-mentioned metal barricade 22 has a predetermined altitude, and its scope can be 0.3, and how rice is to 100 microns.Above-mentioned predetermined altitude can accurately be controlled, with the activity space of restriction determinand.
Comprehensively above-mentioned, slide according to the present invention comprises that a substrate and is arranged at the metal pattern of substrate, and wherein metal pattern comprises above-mentioned metal grid lines, location circumference, reaches metal barricade etc.
In the use-pattern that discloses down by the invention process aspect.Please refer to Fig. 2 and Fig. 4, the recessed space that the metal barricade 22 of second slide 2 is defined can be in order to accept a biological corpse or other object for laboratory examination and chemical testing that contains determinand.Again first slide 1 is placed towards second slide 2 with the face with metal grid lines 12, so that a unnecessary biological corpse or other object for laboratory examination and chemical testing is discharged from opening 23.By first slide 1 and second slide, 2 formed slide groups, follow-up can the observation by optical microscope, and finish the counting of determinand.
Wherein, therefore metal grid lines 12 and metal barricade 22 have high-precision characteristic because be by being combined to form of vacuum evaporation, sputter, plating or aforesaid way, and can control height exactly; And compared to known high precision lapping mode, the higher and tool of the output of the mode of vacuum evaporation, sputter, plating is advantage cheaply.Moreover, compared to known biological counting slide group, the metal grid lines 12 of the available predetermined altitude of the formed slide group of the present invention is based in the recessed space of being defined by metal barricade 22, shortening the distance of determinand and metal grid lines 12 by this, therefore can effectively increase determinand and metal grid lines 12 and fall into probability within the microscopical field depth simultaneously.
Comprehensively above-mentioned, slide formation method of the present invention can reach high yield and have advantage cheaply, and can reduce between determinand and the mesh lines distance so that determinand and mesh lines fall into simultaneously in the microscopical field depth.
The above embodiment only is for technological thought of the present invention and characteristics are described, its purpose is familiar with technical field person and can be understood content of the present invention and implement according to this making, when can not with qualification claim of the present invention, be that all equalizations of doing according to disclosed spirit change or modification, must be encompassed in the claim of the present invention.
Claims (16)
1. slide comprises:
One substrate; And
One metal pattern is arranged at this substrate.
2. slide as claimed in claim 1 is characterized in that this metal pattern comprises many strip metals mesh lines, and those metal grid lines define a plurality of corpse or other object for laboratory examination and chemical testing and calculate the district.
3. slide as claimed in claim 2 is characterized in that, this corpse or other object for laboratory examination and chemical testing calculates the district and is rectangle.
4. slide as claimed in claim 2 is characterized in that, this metal pattern also comprises a setting circle week.
5. slide as claimed in claim 1 is characterized in that, this metal pattern comprises a metal barricade, and this metal barricade has at least one opening.
6. slide as claimed in claim 1 is characterized in that, this metal pattern have 1 how rice to 100 microns predetermined altitude.
7. slide as claimed in claim 1 is characterized in that, the material of this substrate is glass or acryl.
8. slide as claimed in claim 1 is characterized in that, this substrate is transparent.
9. slide formation method comprises:
One substrate is provided; And
One metal pattern is set in this substrate, wherein this metal pattern forms by the combination of vacuum evaporation, sputter, plating or aforesaid way.
10. slide formation method as claimed in claim 9 is characterized in that this metal pattern comprises many strip metals mesh lines, and those metal grid lines define a plurality of corpse or other object for laboratory examination and chemical testing and calculate the district.
11. slide formation method as claimed in claim 10 is characterized in that, this corpse or other object for laboratory examination and chemical testing calculates the district and is rectangle.
12. slide formation method as claimed in claim 10 is characterized in that, this metal pattern also comprises a setting circle week.
13. slide formation method as claimed in claim 9 is characterized in that, this metal pattern comprises a metal barricade, and this metal barricade has at least one opening.
14. slide formation method as claimed in claim 9 is characterized in that, this metal pattern have 1 how rice to 100 microns predetermined altitude.
15. slide formation method as claimed in claim 9 is characterized in that, the material of this substrate is glass or acryl.
16. slide formation method as claimed in claim 9 is characterized in that, this substrate is transparent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100490724A CN101859022B (en) | 2009-04-09 | 2009-04-09 | Slide and forming method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100490724A CN101859022B (en) | 2009-04-09 | 2009-04-09 | Slide and forming method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101859022A true CN101859022A (en) | 2010-10-13 |
| CN101859022B CN101859022B (en) | 2012-07-25 |
Family
ID=42945021
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100490724A Active CN101859022B (en) | 2009-04-09 | 2009-04-09 | Slide and forming method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101859022B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109313175A (en) * | 2016-03-08 | 2019-02-05 | 瑞可利有限公司 | The simple detection kit of sperm, device and the method simply detected for implementing sperm |
| CN110312473A (en) * | 2016-12-21 | 2019-10-08 | 艾森利克斯公司 | For authenticating the device and method and its use of sample |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2355332Y (en) * | 1998-09-02 | 1999-12-22 | 黄文秋 | Track glass |
| CN2377532Y (en) * | 1999-06-10 | 2000-05-10 | 中国人民解放军第二军医大学 | Microfluorecyte counting plate |
| DE10003588C2 (en) * | 2000-01-25 | 2002-10-02 | Sl Microtest Wissenschaftliche | Method of isolating part of a layer of biological material |
| CN1500659A (en) * | 2002-11-19 | 2004-06-02 | 张秋墩 | Method for manufacturing shaped metal design on nonmetal article |
| CN200972447Y (en) * | 2006-11-14 | 2007-11-07 | 南京航空航天大学 | Microflow field sensor for micro real-time investigating particle in flow |
-
2009
- 2009-04-09 CN CN2009100490724A patent/CN101859022B/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109313175A (en) * | 2016-03-08 | 2019-02-05 | 瑞可利有限公司 | The simple detection kit of sperm, device and the method simply detected for implementing sperm |
| CN109313175B (en) * | 2016-03-08 | 2020-11-03 | 瑞可利有限公司 | Simple sperm detection kit, device and method for implementing simple sperm detection |
| CN110312473A (en) * | 2016-12-21 | 2019-10-08 | 艾森利克斯公司 | For authenticating the device and method and its use of sample |
| CN110312473B (en) * | 2016-12-21 | 2023-04-07 | 艾森利克斯公司 | Apparatus and method for authenticating a sample and use thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101859022B (en) | 2012-07-25 |
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