CN101857644A - Alcohol-Alkali Extraction and Production Technology of Asparagine Polysaccharide - Google Patents

Abstract

The invention discloses an alcohol-alkali extraction method and a production process of asparagus polysaccharide. The extraction method is as follows: take asparagus decoction pieces, add an extraction solvent with an ethanol concentration of 5 to 50% according to the mass volume ratio of 1:15 to 1:6, adjust the pH to 5 to 13, soak for 0.5 to 2.5 hours, and place it in a water bath of 60 to 100 Extract at ℃ for 0.5-2.5 hours, filter, treat the residue at least once as above, and combine the filtrates to obtain the extract. The production process adopting this extraction method also includes the following steps: concentrating the extract, adding 95% ethanol for alcohol precipitation, discarding the supernatant; adding the same amount of water as the concentrated solution, heating and dissolving the precipitate, and centrifuging to collect the supernatant; Precipitate with 95% ethanol in the concentrated solution, centrifuge and discard the supernatant; add an appropriate amount of 95% ethanol to stir, centrifuge and then discard the supernatant; add an appropriate amount of absolute ethanol to stir, collect the precipitate after centrifugation, and freeze-dry. The invention obviously improves the extraction rate and total sugar content of the asparagus polysaccharide.

Description

Alcohol-Alkali Extraction and Production Technology of Asparagine Polysaccharide

technical field

The invention relates to an alcohol-alkali extraction method and a production process of aspartame polysaccharide.

Background technique

Asparagus Cochinchinensis polysaccharide, as a chicken vaccine immune enhancer, can significantly improve the protection rate and disease resistance of the vaccine, and has a broad application prospect. However, the current classical water extraction and alkaline water extraction methods for extracting asparagus polysaccharides have the problems of low extraction rate and low total sugar content, so that the production efficiency of asparagus polysaccharides is low and the supply is insufficient, making it difficult to meet market demand.

Contents of the invention

The technical problem to be solved by the present invention is to provide an alcohol-alkali extraction method and production process of aspartame polysaccharide with high extraction rate and high total sugar content.

In order to solve the above-mentioned technical problems, the alcohol-alkali extraction method of asparagus polysaccharide of the present invention adopts following technical scheme: get asparagus decoction pieces, add the extraction solvent that ethanol concentration is 5～50% by mass volume ratio 1: 15～1: 6, adjust pH to 5-13, soak for 0.5-2.5 hours, then extract in a water bath at 60-100°C for 0.5-2.5 hours, filter, treat the residue at least once as above, and combine the filtrates to obtain the extract.

The production technology of asparagus polysaccharide adopting the above-mentioned alcohol-alkali extraction method also includes the following steps: concentrating the extract to obtain a concentrated solution containing 0.5g/ml crude drug, adding 95% ethanol with a volume 5 times that of the concentrated solution for alcohol precipitation, discarding Supernatant; add the same amount of water as the concentrate, heat to dissolve the precipitate, centrifuge to collect the supernatant; then add 95% ethanol with a volume 5 times that of the concentrate for alcohol precipitation, centrifuge and discard the supernatant; add an appropriate amount of 95% ethanol to stir well, and centrifuge Then discard the supernatant; add an appropriate amount of absolute ethanol to stir evenly, collect the precipitate after centrifugation, and freeze-dry to obtain asparagine polysaccharide.

The extraction rate and total sugar content of the alcohol-alkali extraction method and the production process of the asparagine polysaccharide of the present invention are higher than the current classic water extraction method and alkaline water extraction method, and the application to the production of the asparagine polysaccharide can improve efficiency and meet market demand.

Detailed ways

Below is the embodiment of the alcohol-alkali extraction method of aspartame polysaccharide of the present invention:

Example 1

Take 100g of Asparagus decoction pieces dried to constant weight, add 1500ml of extraction solvent with ethanol concentration of 10%, adjust pH to 9, soak for 1.5 hours, extract in water bath at 80°C for 2.5 hours, filter with four layers of gauze, and treat the residue once as above , Combine the two filtrates to obtain the extract.

Example 2

Take 100g of Asparagus decoction pieces dried to constant weight, add 600ml of extraction solvent with ethanol concentration of 5%, adjust the pH to 13, soak for 2.5 hours, extract in water bath at 60°C for 1.5 hours, filter with four layers of gauze, and treat the residue once as above , Combine the two filtrates to obtain the extract.

Example 3

Take 100g of Asparagus decoction pieces dried to constant weight, add 1000ml of extraction solvent with ethanol concentration of 30%, adjust pH to 5, soak for 0.5 hours, extract in water bath at 100°C for 0.5 hours, filter with four layers of gauze, and treat the residue once as above , Combine the two filtrates to obtain the extract.

Example 4

Take 100g of Asparagus decoction pieces dried to constant weight, add 800ml of extraction solvent with ethanol concentration of 50%, adjust pH to 7, soak for 2.0 hours, extract in water bath at 90°C for 1.0 hour, filter with four layers of gauze, and treat the residue once as above , Combine the two filtrates to obtain the extract.

The following is a comparison example of the classic water extraction method and alkaline water extraction method of aspartame polysaccharide:

The classic water extraction method of control 1 asparagine polysaccharide

Take 100 g of Radix Asparagus decoction pieces dried to constant weight, add 1500 ml of distilled water, decoct and extract for 2.5 hours, filter through four layers of gauze, treat the residue once as above, and combine the two filtrates to obtain the extract.

Alkaline Water Extraction Method of Control 2 Asparagine Polysaccharide

Take 100g of Asparagus decoction pieces dried to constant weight, add 1500ml of distilled water, adjust the pH to 9, soak for 1.5 hours, then extract in a water bath at 80°C for 2.5 hours, filter through four layers of gauze, treat the residue once as above, and combine the two filtrates to obtain the extraction liquid.

The production technology of embodiment 5 asparagine polysaccharides

Concentrate the extracts of the above-mentioned Examples 1 to 4 and Controls 1 and 2 respectively to contain 0.5 g/ml of crude drug to obtain 200 ml of concentrated solution, add 95% ethanol with a volume 5 times that of the concentrated solution for alcohol precipitation, add slowly, and stir while adding , stand overnight at room temperature, then discard the supernatant; add 200mL of distilled water to heat to dissolve the precipitate, centrifuge at 3000r/min for 10min, and collect the supernatant; then add 95% ethanol with a volume 5 times that of the concentrated solution for alcohol precipitation, place at room temperature overnight, 3000r Centrifuge for 10 min at 1/min, discard the supernatant; add an appropriate amount of 95% ethanol and stir evenly, centrifuge as before, then discard the supernatant; add appropriate amount of absolute ethanol, stir evenly, then centrifuge, finally collect the precipitate, freeze-dry to obtain the asparagine polysaccharide.

Determination and Results of Asparagine Polysaccharide

Adopt the phenol-sulfuric acid method to measure the Asparagus polysaccharide content obtained in above-mentioned embodiment, wherein, crude polysaccharide extraction rate is calculated according to the following formula:

Extraction rate of crude polysaccharide = mass of crude polysaccharide / mass of asparagus decoction pieces × 100%.

The test results are listed as follows:

Examples or comparative examples 1 2 3 4 Control 1 Control 2 Crude polysaccharide extraction rate (%) 18.64 13.95 15.74 14.98 2.99 9.01 Total sugar content (%) 89.80 89.34 91.55 92.60 41.13 81.95

It can be seen that the extraction and production of asparagus polysaccharides by alcohol-alkali method has significantly improved the extraction rate of crude polysaccharides and the total sugar content, which provides a good process route for the large-scale production of asparagus polysaccharides in the future, and can effectively ensure the immune enhancement of chicken vaccines market supply of agents.

Claims (2)

1. the pure alkali extraction method of a Radix Asparagi polysaccharide, it is characterized in that this extraction method comprises the steps: to get the Radix Asparagi medicine materical crude slice, adding alcohol concn by mass volume ratio 1: 15～1: 6 is 5～50% extraction solvent, regulate pH to 5～13, soak after 0.5～2.5 hour and extracted 0.5～2.5 hour for 60～100 ℃ in water-bath, filter, the same processing of residue at least once merges each time filtrate and gets extracting solution.

2. according to the production technique of the pure alkali extraction method of the described Radix Asparagi polysaccharide of claim 1, it is characterized in that this technology also comprises the steps: to concentrate described extracting solution and gets concentrated solution to containing crude drug 0.5g/ml, add 5 times of 95% ethanol of volume and carry out alcohol precipitation, discard supernatant to concentrated solution; Add with the water heating for dissolving precipitation that concentrates liquid measure, centrifugal collection supernatant; Add 5 times of 95% ethanol of volume again and carry out alcohol precipitation, the centrifugal supernatant that discards to concentrated solution; Add an amount of 95% ethanol and stir evenly, the centrifugal supernatant of abandoning again; Add an amount of dehydrated alcohol and stir evenly, centrifugal back collecting precipitation, lyophilize promptly gets the Radix Asparagi polysaccharide.