CN101845078B - Method for extracting protein from wastewater of potato starch - Google Patents
Method for extracting protein from wastewater of potato starch Download PDFInfo
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- CN101845078B CN101845078B CN2010101489943A CN201010148994A CN101845078B CN 101845078 B CN101845078 B CN 101845078B CN 2010101489943 A CN2010101489943 A CN 2010101489943A CN 201010148994 A CN201010148994 A CN 201010148994A CN 101845078 B CN101845078 B CN 101845078B
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Abstract
The invention provides a method for extracting protein from wastewater of potato starch, comprising the following steps: standing and precipitating potato starch wastewater, and centrifuging and filtering; adding an acidic reagent to adjust the pH value, centrifuging and filtering to obtain protein, washing, centrifuging and filtering again to obtain washed protein 1; adding an alkali reagent in supernate after centrifugation and extraction of the protein 1, adjusting the pH value to be 8.50, centrifuging and filtering to obtain protein, washing, centrifuging and filtering again to obtain washed protein 2; and mixing the washed protein 1 and washed protein 2, adding water with one time volume of the mixed solution, mixing evenly, adopting NaOH solution to adjust the pH value to be 7, preparing into neutral protein mixed solution, and drying the mixed solution under a vacuum condition to obtain the dried protein product. The invention does not use any coagulant or flocculant, can separate protein by adjusting the pH value, centrifugation speed and centrifugation time of the wastewater, solves the environment pollution problem caused by the wastewater in the process of producing the potato starch, and has obvious economic and social benefits.
Description
Technical field
The present invention relates to the proteins extraction technical field, particularly a kind of from yam starch waste water method for extracting proteins.
Background technology
According to estimates, the protein market in the whole world is up to 3,000,000,000 dollars, and what be widely used in food-processing has: milk-protein, animal proteinum, vegetable-protein, gelatin etc.Along with the progress and the growth in the living standard of society, people constantly increase proteinic absorption; Because vegetable-protein is comparatively safe, and has health-care effect and enjoy favor.Market based on this reason vegetable-protein is also increasing; It is extremely abundant to have the Methionin that lacks in multiple the isostatic amino acid composition, particularly cereal as the Rhizoma Solani tuber osi protein of one of vegetable-protein, can compare favourably with soybean protein, and high nutritive value is arranged.
There is yam starch manufacturer of the thousands of family of large, medium and small type in China, the potato amount of annual processing is about 4,000,000 tons, the waste water of discharging in the yam starch course of processing is about 700,000 tons, and contains materials such as water-soluble protein 0.1～0.2%, starch 0.2～0.3%, Mierocrystalline cellulose 0.1～0.2%, total reducing sugar 0.05～0.15% in these waste water.If these starch wastewaters are directly discharged, not only can very fast souring rot, make environment water anoxic, hydrobiont death by suffocation, and wasted the resource of many preciousnesses.Therefore, many companies are developing protein that is obtained by yam starch waste water and products thereof; For example, utilize the albumen in the membrane separation technique recovery yam starch waste water, the process operation expense is low, simple to operate, protein extracting ratio is high, but the equipment one-time investment is big, and it is fast that film is changed speed, applies difficulty.Method such as coagulation, precipitation, process operation expense height, protein extracting ratio is low and of poor quality, also is difficult to apply.
Summary of the invention
Technical problem to be solved by this invention is to realize the comprehensive utilization of yam starch waste water resource, solve the waste water that produces in the starch processing to pollution that environment caused, make refuse can access recycling simultaneously, increase value-added content of product, can obtain method for extracting protein in the yam starch waste water of highly purified protein extract by yam starch waste water thereby provide a kind of.
Technical problem of the present invention solves by following technical proposals:
Method for extracting protein in a kind of yam starch waste water may further comprise the steps:
(1) precipitation and filtration
Earlier yam starch waste water is staticly settled in settling tank, filter by centrifugal screen again;
(2) acid for adjusting pH value extracts protein from yam starch waste water
Adding acid reagent adjusting pH value in the waste water after (1) precipitation, filtration is 4.75, and centrifuging obtains protein, and with 2～3 times water washing, recentrifuge filters, and obtains washed protein 1;
(3) alkali is regulated the pH value, extracts protein from yam starch waste water
Centrifugal and extract and to add alkaline reagents in the supernatant liquor behind the protein 1 to regulate pH value be 8.50 through above-mentioned (2), centrifuging obtains protein, and with 2～3 times water washing, the recentrifuge filtration obtains washed protein;
(4) mixing of protein extract is with dry
After washed protein 1 that step (2), (3) are obtained and washed protein 2 are mixed, the water that adds 1 times of amount of mixed solution mixes, and regulating the pH value with NaOH solution is 7, makes neutral protein matter mixed solution, mixed solution carries out drying under vacuum condition, obtain the exsiccant proteinaceous product.
The described waste water time of staticly settling of step (1) is 2-3 hour, and the aperture of described centrifugal screen is 80 μ m.
Acid reagent is HCl described in the step (2), and reagent concentration is 1.0mol/L.
Alkaline reagents is NaOH described in the step (3), and reagent concentration is 1.0mol/L.
Reaction, the centrifugal condition of described step (2), (3) are: 20～25 ℃ of temperature, speed 3000r/min, time 15min～20min.
Mixing and drying conditions in the described step (4) are: pH of mixed value 7.0, and vacuum tightness-0.060～-0.075MPa, 20～25 ℃ of temperature.
The invention has the advantages that:
1. under the condition of not using any cohesion or flocculation agent, albumen is separated by pH value, centrifugal speed and the centrifugation time of regulating waste water;
2. realize separation from yam starch waste water, the industrialization of extracting potato protein, realize the utilization again of potato waste water resource, increase value-added content of product;
3. solve wastewater produced pollution on the environment in the yam starch process of manufacture, economical, societal benefits are remarkable.
Protein extract result according to the embodiment of the invention 1 acquisition from yam starch waste water, confirmed that method of the present invention can obtain highly purified protein extract by yam starch waste water, be lower than 60% and existing processes makes proteinic purity.
Table 1 is from the protein extract analytical results of yam starch waste water
Description of drawings
Fig. 1 is a process flow sheet of the present invention;
Fig. 2 is the best sour iso-electric point pI of protein;
Fig. 3 is the best alkali iso-electric point of protein pI.
Embodiment
Method for extracting protein in a kind of yam starch waste water comprises precipitation, filtration, regulates processes such as pH value, separation, washing, mixing, vacuum-drying, packing.Concrete steps are:
1, the precipitation of waste water and filtration
Contain a large amount of water soluble proteins, fiber and remaining starch etc. in the waste water after the yam starch processing, after earlier waste water being left standstill 2-3 hour in settling tank, selecting the aperture again for use is the centrifugal screen filtration of 80 μ m, wherein fiber and other solid nonprotein class impurity are removed, thereby guaranteed to extract proteinic purity.
2, regulate the acid ph value of waste water and extract protein
Protein is to be interconnected by peptide chain by different amino acid to form.The characteristics of solubleness minimum during according to isoelectric points of proteins are by adding acid, adding alkali and regulate the pH value of waste water and wherein protein is flocculated precipitate.Under 20～25 ℃ of conditions, in the yam starch waste water after step 1 is filtered, add the HCl of 1.0mol/L, the pH value is adjusted to 4.75, and keeps 10min so that it fully reacts.Under 3000r/min, carry out centrifugally, isolate sedimentary protein.With the water washing of the precipitating proteins that obtains, obtain washing and the protein 1 of purifying through recentrifuge with 2～3 times.
3, regulate the alkaline pH value of waste water and extract protein
To drip the NaOH of 1.0mol/L through the supernatant liquor behind above-mentioned steps 2 adjustment of acidity pH values and the extraction protein down again, being adjusted to the pH value is 8.50, and the protein soln of formation is placed 10min.Carry out centrifugally at 20～25 ℃ of condition 3000r/min, isolate sedimentary protein.With the water washing of the precipitating proteins that obtains, obtain washing and the protein 2 of purifying through recentrifuge with 2～3 times.
4. the mixing of protein extract is with dry
Above-mentioned protein that obtains 1 and protein 2 are merged the water that the back adds 1 times of amount, and make its uniform mixing.The NaOH that adds concentration then and be 1.0mol/L in the protein mixed solution is adjusted to neutrality.Protein mixed solution after the neutralization is-0.060 in vacuum tightness～-0.075MPa, temperature is to carry out vacuum-drying under 20～25 ℃ of conditions, obtains the exsiccant proteinaceous product.
Below in conjunction with embodiment the present invention is described in further details:
Embodiment 1
1, the precipitation of waste water and filtration
After earlier waste water being left standstill 2 hours in settling tank, selecting the aperture again for use is the centrifugal screen filtration of 80 μ m, and wherein fiber and other solid nonprotein class impurity are removed.
2, regulate the acid ph value of waste water and extract protein
Under 20 ℃ of conditions, learn from else's experience above-mentioned 1 precipitation, filtering yam starch waste water 500mL, the HCl of adding 1.0mol/L regulates pH value to 4.75, and keeps 10min, and it is fully reacted.Under 3000r/min, carry out centrifugally, obtain precipitating proteins 12.4g,, obtain washing and the protein 1 of purifying through recentrifuge with the precipitating proteins that obtains 24.8g water washing.
3. regulate the alkaline pH value of waste water and extract protein
To drip the NaOH of 1.0mol/L under 20 ℃ of conditions through the supernatant liquor 500mL after step 2 is regulated the acid ph value of waste water and extracted protein again, and regulate pH value to 8.50, the protein soln of formation is placed 10min.Under 3000r/min, carry out centrifugally, obtain precipitating proteins 4.3g,, obtain washing and the protein 2 of purifying through recentrifuge with the precipitating proteins that obtains 8.6g water washing.
4. the mixing of protein extract is with dry
Above-mentioned 2,3 protein that obtain 1 and protein 2 are merged 16.7g altogether, add the water of 16.7g, and make its uniform mixing.The NaOH that slow adding concentration is 1.0mol/L in the protein mixed solution is adjusted to neutrality then.With the protein mixed solution after the neutralization is-0.060MPa that temperature is to carry out vacuum-drying under 20 ℃ of conditions, obtains Protein content and reaches 83.50%, extraction rate reached 83.0% in vacuum tightness.
Embodiment 2:
1, the precipitation of waste water and filtration
After earlier waste water being left standstill 3 hours in settling tank, selecting the aperture again for use is the centrifugal screen filtration of 80 μ m, wherein fiber and other solid nonprotein class impurity is removed, thereby guaranteed to extract proteinic purity.
2, regulate the acid ph value of waste water and extract protein
Under 25 ℃ of conditions, the yam starch waste water 500mL after learn from else's experience above-mentioned 1 precipitation, the filtration, the HCl of adding 1.0mol/L is adjusted to 4.75 with the pH value, and keeps 20min, and it is fully reacted.Under 3000r/min, carry out centrifugally, isolate precipitating proteins 12.2g.With the precipitating proteins that obtains 36.6g water washing, obtain washing the also protein 1 of purifying through recentrifuge.
3. regulate the alkaline pH value of waste water and extract protein
To be added dropwise to the NaOH of 1.0mol/L under 25 ℃ of conditions through the supernatant liquor 500mL behind above-mentioned steps 2 adjustment of acidity pH values and the extraction protein again, being adjusted to the pH value is 8.50, and the protein soln of formation is placed 10min.Under 3000r/min, carry out centrifugally, isolate precipitating proteins 4.1g.With the precipitating proteins that obtains 12.3g water washing, obtain washing the also protein 2 of purifying through recentrifuge.
4. the mixing of protein extract is with dry
Above-mentioned protein that obtains 1 and protein 2 are merged 16.3g altogether, add the water of 16.3g, and make its uniform mixing.The NaOH that slow adding concentration is 1.0mol/L in the protein mixed solution is adjusted to neutrality then.With the protein mixed solution after the neutralization is-0.075MPa that temperature is to carry out vacuum-drying under 25 ℃ of conditions, obtains the exsiccant proteinaceous product, obtains Protein content and reaches 83%, extraction rate reached 80.5% in vacuum tightness.
Claims (6)
1. method for extracting protein in the yam starch waste water is characterized in that being undertaken by following step:
(1) precipitation and filtration
Earlier yam starch waste water is staticly settled in settling tank, filter by centrifugal screen again;
(2) acid for adjusting pH value extracts protein from yam starch waste water
Adding acid reagent adjusting pH value in the waste water after step (1) precipitation, filtration is 4.75, places 10min, and centrifuging obtains protein, and with 2～3 times water washing, recentrifuge filters, and obtains washed protein 1;
(3) alkali is regulated the pH value, extracts protein from yam starch waste water
Centrifugal and extract and to add alkaline reagents in the supernatant liquor behind the protein 1 to regulate pH value be 8.50 through above-mentioned (2), place 10min, centrifuging obtains protein, and with 2～3 times water washing, the recentrifuge filtration obtains washed protein 2;
(4) mixing of protein extract is with dry
After washed protein 1 that step (2), (3) are obtained and washed protein 2 are mixed, the water that adds 1 times of amount of mixed solution mixes, and regulating the pH value with NaOH solution is 7, makes neutral protein matter mixed solution, mixed solution carries out drying under vacuum condition, obtain the exsiccant proteinaceous product.
2. method for extracting protein in the yam starch waste water according to claim 1 is characterized in that the described waste water time of staticly settling of step (1) is 2～3 hours, and the aperture of described centrifugal screen is 80 μ m.
3. method for extracting protein in the yam starch waste water according to claim 1 is characterized in that acid reagent is HCl described in the step (2), and reagent concentration is 1.0mol/L.
4. method for extracting protein in the yam starch waste water according to claim 1 is characterized in that alkaline reagents is NaOH described in the step (3), and reagent concentration is 1.0mol/L.
5. method for extracting protein in the yam starch waste water according to claim 1 is characterized in that the reaction of described step (2), (3), centrifugal condition are: 20～25 ℃ of temperature, speed 3000r/min, time 15min～20min.
6. method for extracting protein in the yam starch waste water according to claim 1 is characterized in that mixing and the drying conditions in the described step (4) is: pH of mixed value 7.0, and vacuum tightness-0.060～-0.075MPa, 20～25 ℃ of temperature.
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| CN2010101489943A CN101845078B (en) | 2010-04-14 | 2010-04-14 | Method for extracting protein from wastewater of potato starch |
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| CN2010101489943A CN101845078B (en) | 2010-04-14 | 2010-04-14 | Method for extracting protein from wastewater of potato starch |
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| CN101845078B true CN101845078B (en) | 2011-08-31 |
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102504011B (en) * | 2011-12-01 | 2014-05-28 | 中国食品发酵工业研究院 | Method for separating and preparing potato protein powder from potato juice |
| CN102690031A (en) * | 2012-06-27 | 2012-09-26 | 山东博洋环境资源有限公司 | Treatment method for recycling potato starch production factory wastewater |
| CN106819699B (en) * | 2017-01-16 | 2018-10-23 | 北京联合大学 | Potato Normal juice, juice beverage and preparation method thereof |
| WO2017181949A1 (en) * | 2016-04-20 | 2017-10-26 | 北京联合大学 | Potato products and processing method therefor |
| CN106916201A (en) * | 2017-02-28 | 2017-07-04 | 嵊州市派特普科技开发有限公司 | The method that albumen is extracted from potato starch processing-waste |
| CA3075624C (en) * | 2017-10-31 | 2021-11-30 | Cooperatie Avebe U.A. | Potato protein based fibrous structures and food items comprising the same |
| CN107651758B (en) * | 2017-11-15 | 2020-06-02 | 应城市新都化工有限责任公司 | Method for preparing organic fertilizer by using starch wastewater |
| CN107651759A (en) * | 2017-11-15 | 2018-02-02 | 广西南宁荣威德新能源科技有限公司 | A kind of method for handling starch wastewater |
| CN110627898B (en) * | 2019-11-08 | 2023-07-25 | 安徽天凯生物科技有限公司 | Extraction process of potato trypsin inhibitor |
| CN111909233A (en) * | 2020-05-27 | 2020-11-10 | 中国农业大学 | Method for recovering protein in byproduct of sweet potato starch production and sweet potato processing method |
| CN115504598B (en) * | 2022-09-21 | 2024-05-14 | 中国电建集团昆明勘测设计研究院有限公司 | Wastewater treatment and recycling process for gelatin production workshop |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1217890A (en) * | 1997-11-26 | 1999-06-02 | 中国科学院新疆化学研究所 | Method for recovering protein from waste water in production of starch from potatoes |
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| CN100391870C (en) * | 2005-04-22 | 2008-06-04 | 黄磊 | Circulation utilizing technology for waste water and water dreg from potato starch production process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1217890A (en) * | 1997-11-26 | 1999-06-02 | 中国科学院新疆化学研究所 | Method for recovering protein from waste water in production of starch from potatoes |
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