CN101263859A - Method for extracting fish protein from wastewater for processing aquatic product - Google Patents
Method for extracting fish protein from wastewater for processing aquatic product Download PDFInfo
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- CN101263859A CN101263859A CNA2007100677036A CN200710067703A CN101263859A CN 101263859 A CN101263859 A CN 101263859A CN A2007100677036 A CNA2007100677036 A CN A2007100677036A CN 200710067703 A CN200710067703 A CN 200710067703A CN 101263859 A CN101263859 A CN 101263859A
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- albumen
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- waste water
- wastewater
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- 238000000034 method Methods 0.000 title claims abstract description 56
- 239000002351 wastewater Substances 0.000 title claims abstract description 43
- 108010028690 Fish Proteins Proteins 0.000 title abstract description 9
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 48
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 48
- 241000251468 Actinopterygii Species 0.000 claims abstract description 41
- 239000002245 particle Substances 0.000 claims abstract description 26
- 239000006228 supernatant Substances 0.000 claims abstract description 11
- 150000002632 lipids Chemical class 0.000 claims abstract description 9
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 230000001105 regulatory effect Effects 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 22
- 239000000047 product Substances 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000001914 filtration Methods 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 230000008021 deposition Effects 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 239000007791 liquid phase Substances 0.000 claims 1
- 235000013372 meat Nutrition 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract 4
- 239000012460 protein solution Substances 0.000 abstract 2
- 238000009877 rendering Methods 0.000 abstract 1
- 235000019688 fish Nutrition 0.000 description 36
- 235000018102 proteins Nutrition 0.000 description 35
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 11
- 238000005119 centrifugation Methods 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 5
- 238000004062 sedimentation Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 210000003205 muscle Anatomy 0.000 description 4
- 239000008394 flocculating agent Substances 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 230000006920 protein precipitation Effects 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 239000004365 Protease Substances 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000003531 protein hydrolysate Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 235000015170 shellfish Nutrition 0.000 description 2
- 239000004071 soot Substances 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- WXHLLJAMBQLULT-UHFFFAOYSA-N 2-[[6-[4-(2-hydroxyethyl)piperazin-1-yl]-2-methylpyrimidin-4-yl]amino]-n-(2-methyl-6-sulfanylphenyl)-1,3-thiazole-5-carboxamide;hydrate Chemical compound O.C=1C(N2CCN(CCO)CC2)=NC(C)=NC=1NC(S1)=NC=C1C(=O)NC1=C(C)C=CC=C1S WXHLLJAMBQLULT-UHFFFAOYSA-N 0.000 description 1
- 241000273930 Brevoortia tyrannus Species 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241001417494 Sciaenidae Species 0.000 description 1
- 241000269821 Scombridae Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241001125843 Trichiuridae Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- XTEGARKTQYYJKE-UHFFFAOYSA-N chloric acid Chemical compound OCl(=O)=O XTEGARKTQYYJKE-UHFFFAOYSA-N 0.000 description 1
- 229940005991 chloric acid Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 235000020640 mackerel Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
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Abstract
The invention provides a method for extracting fish proteins from fishery manufacturing wastewater, comprising steps as follows: removing lipid floating on the wastewater first; conducting an isoelectric point determination for wastewater with particles of fish meat removed; regulating the pH values of the wastewater until to the isoelectric point, which enables the proteins to be settled; removing supernatant to acquire protein solutions; finally gaining wet proteins from the protein solutions through a centrifugal method. The method for extracting fish proteins from fishery manufacturing wastewater is characterized in that: the isoelectric point determination is carried out first, then a separation method through isoelectric points is applied for the protein, rendering the proteins to be settled, and finally a centrifugal separation method is used to acquire the proteins. The method for extracting fish proteins from fishery manufacturing wastewater has the advantages of effectively extracting fish proteins from the fishery manufacturing wastewater, small investment, simple operation, low cost, high extraction rate and waste reuse.
Description
Technical field
What the present invention relates to is the extracting method of the useless water fish albumen of a kind of aquatic products processing.
Background technology
Contain flesh of fish particulate and water-soluble fish protein in the aquatic products processing waste water.In the juice as fish gruel, fish meat sheet rinsing and boiling, general albumen total content is at 0.5%-3%.According to environmental protection requirement, the discharging of these waste water must just can be carried out through handling.The notion of the said flesh of fish of the present invention and fish albumen is meat and the albumen of whole aquatic livestocks.Current now aquatic products processing wastewater treatment way is, adopt methods such as anaerobic process, aerobic method, air supporting and biochemical treatment earlier the organic substances such as protein in the waste water to be settled into mud cake, to be dissolved in precipitations such as protein in the waste water with sedimentation agent again, upper strata cleaning waste water be reached enter after the discharge standard marine.Then resulting mud cake and sediment are removed most of moisture in the precipitation through filter press press filtration or dewaterer dehydration.At last the filter mud cake being transported to the soot handles.This processing method at first is the appropriate litigation fees height, and year sewage treatment equipment of disposal ability more than ten thousand tons not only need be invested nearly ten million yuan, and the annual operating cost of handling more reaches more than 300 ten thousand yuan, has increased entreprise cost.Though to be waste water reach discharge standard but protein is transported to the soot as solid waste for next, can pollute equally environment.Also wasted good fish protein resource at last.If therefore this part albumen is extracted from waste water, can not only obtain some high-quality fish albumen, can also reduce the wastewater treatment expense, and significantly reduce pollution environment.
For extract albumen from waste water, the method that adopts mainly contains by centrifugal separation, vacuum concentration method and flocking settling method at present.Wherein centrifugal separation is with solid protein particle in the centrifugal force sedimentation juice, but this method can not be extracted the water-solubility protein in the juice; The vacuum concentration method is to extract albumen with the process that makes liquid concentration after the vacuum evaporation, but this method energy consumption is very big; The flocculating agent sedimentation be with flocculating agent with protein deposition, essential add flocculating agent and the gained purity of protein not high.As seen the method for still not having at present albumen in a kind of extraction waste water of easy economy can be applicable to the extraction to the useless water fish albumen of aquatic products processing.
Summary of the invention
At above-mentioned deficiency, technical problem to be solved by this invention is to make up a kind ofly both the albumen in the aquatic products processing wastewater all to be reclaimed the extracting method of the useless water fish albumen of the aquatic products processing that technology is simple again, operating cost is low.
The extracting method of the useless water fish albumen of aquatic products processing provided by the invention, remove the lipid that floats on the waste water earlier, subsequently the waste water of removing flesh of fish particle is carried out isoelectric point determination, pH value to the isoelectric point of regulating waste water again makes protein deposition, remove supernatant then and get protein liquid, at last the gained protein liquid is obtained wet albumen with centrifugal method.
The extracting method of the useless water fish albumen of aquatic products processing provided by the invention after removing the lipid that floats on the waste water, will be oppressed particle separation with the method for filtering earlier and go out waste water.But flesh of fish particle is more tiny in the waste water, below 100 orders, though or flesh of fish particle is bigger measures when fewer, then needn't separate and directly carry out isoelectric point determination.
The extracting method of the useless water fish albumen of aquatic products processing provided by the invention, to filter with the method for filtering flesh of fish particle get albumen powder with the method for drying and crushing, when filter flesh of fish particle be the loose flesh of fish particle of muscle, can adopt the way that directly is dried pulverizing to obtain albumen powder.
The extracting method of the useless water fish albumen of aquatic products processing provided by the invention, to filter with the method for filtering flesh of fish particle be hydrolyzed with enzyme process, regulate hydrolysis then and finish the pH value of liquid and make protein deposition, remove supernatant and get protein liquid, at last the gained protein liquid is obtained wet albumen with centrifugal method.
The extracting method of the useless water fish albumen of aquatic products processing provided by the invention can add settling agent when protein deposition speed is slow, as: trichloroacetic acid, mistake chloric acid, sulfosalicylic acid etc. are obviously accelerated its settling velocity.If filtered fluid protein settling speed is not very slow, do not need with settling agent, the proper extension sedimentation time also can reach effect of settling.
The extracting method of the useless water fish albumen of aquatic products processing provided by the invention is with the dry albumen powder that gets of the wet albumen of gained.
Fish albumen is the very high albumen replenishers of a kind of nutritive value, easily digests and assimilates, and for the protein component that replenishes human body, the immunologic function that improves human body has bigger effect.Therefore can the food prepared therefrom nourishing additive agent, join in other food, also can make health food and eat.The extracting method of the useless water fish albumen of aquatic products processing provided by the invention is carried out isoelectric point determination with aquatic products processing waste water earlier, makes albumen precipitation with albumen isoelectric point partition method again, obtains wet albumen with centrifugal separation again.Be the extracting method of the useless water fish albumen of aquatic products processing provided by the invention, can extract the fish albumen in the waste water aquatic products processing effectively, drop into little, simple to operate, cost is low, recovery rate is high, turn waste into wealth.
Description of drawings
Accompanying drawing is the extracting method technology whole process figure of the useless water fish albumen of aquatic products processing provided by the present invention.
The technology whole process of the extracting method of this fish protein from wastewater for processing aquatic product provided by the present invention is:
1, lipid is removed: contain higher lipid in the wastewater from aquatic product process, utilize lipid proportion littler earlier with lipid from waste water In separate.
2, measure and to oppress grain graininess in the waste water: sampling is measured flesh of fish particle size in the waste water, as less than setting The particle size is not then filtered, and (negligible amounts does not filter yet) is as then filtering greater than the particle size of setting.
3, measure isoelectric point: when not filtering, waste water is directly measured isoelectric point.
1. get 7 in the test tube of same specification, according to the form below accurately adds following reagent (mL);
2. fully shake even;
3. add successively processing juice 1mL to above each test tube then, Bian Jiabian shakes, and shakes to leave standstill 5min after even;
4. observe the turbidity of each pipe, in upper table with-,+, ++, +++wait the turbidity of each pipe of symbolic representation. According to mixed Turbidity is judged the isoelectric point of albumen, and the pH value of a most muddy pipe is the isoelectric point of this juice albumen.
4, regulate the pH value to isoelectric point: when utilizing isoelectric point protein with positive and negative charge equate that total electrical charge is zero principle that precipitate, at the pH value of original processing juice, with acid solution or alkali lye adjusting pH near isoelectric points of proteins.
5, protein deposition: produce the protein settling phenomenon during isoelectric points of proteins significantly.Through leaving standstill about 1h, protein is precipitated out from juice, and with the juice layering.Remove supernatant liquor, promptly get protein precipitation liquid 1.Cross slowly and can its settling velocity obviously be accelerated as sinking speed by adding settling agent.
6, centrifugation: protein liquid 1 is carried out centrifugation in centrifuge, regulate more than the centrifuge speed 4000 commentaries on classics/min, albumen is condensed in the Centrifuge Cup bottom behind the centrifugal certain hour, goes supernatant, promptly gets the albumen that wets.
7, drying: after the albumen that will wet freezes,, make albumen powder by freeze drying.
3 ', filter: what waste water need filter after measured then filters with stainless (steel) wire, and graininess meat and juice are separated, and filtrate is the same with the waste water of not doing to filter handles, and promptly enters for the 3rd step.Flesh of fish particle carries out different processing by the difference of meat kind.The flesh of fish particle loose for muscle then obtains albumen powder after drying, the pulverizing.
4 ', enzymolysis:, it is become protein hydrolyzate with protease hydrolytic for the loose flesh of fish particle of non-muscle.For the ease of making the albumen powder product, by the utilization zymolysis technique, with these flesh of fish particle breakdown.
5 ', separate: enzymolysis is finished the liquid branch residue of leaving away.
6 ', regulate the pH value: above-mentioned protein hydrolyzate is regulated the pH value to albumen precipitation occurring.
7 ', albumen precipitation: through leaving standstill about 1h, protein is precipitated out from juice, and with the juice layering.Remove supernatant liquor, promptly get protein precipitation liquid 2.Cross slowly and can its settling velocity obviously be accelerated as sinking speed by adding settling agent.Protein liquid 2 can carry out centrifugation with protein liquid 1, also can carry out centrifugation separately, the further dry albumen powder that gets of wet albumen.
The specific embodiment
1, the rotten poaching wastewater of fish contains protein 0.52% through measuring.Obtaining larger particles flesh of fish particle through filtration is 5.4%, adds papain 3000IU/g hydrolysis, regulates pH and makes protein precipitation, staticly settles the back abandoning supernatant and gets protein liquid 2; Filtered fluid is surveyed isoelectric point, and getting pH to 5.3～5.6 is isoelectric point, regulates pH to 5.3～5.6 and occurs deposited phenomenon significantly, and is slow excessively because of protein settling speed, adds an amount of settling agent, leaves standstill more than the 1hr, and abandoning supernatant gets protein liquid 1; Mixed protein liquid 1 carries out centrifugation with protein liquid 2, and centrifuge speed is 4200 commentaries on classics/min, and time 10min obtains wet albumen 2%, and drying gets dried albumen 0.50%.Protein recovery 96.15% in the waste water.
2, shellfish boiling waste water seldom has particle shellfish meat, surveys isoelectric point through the fat back of removing come-up, getting about pH to 5.3 is isoelectric point, regulate pH to 5.3 and occur deposited phenomenon significantly, slow excessively because of protein settling speed, add an amount of settling agent, leave standstill more than the 1hr, abandoning supernatant gets protein liquid and carries out centrifugation, and centrifuge speed is 4200 commentaries on classics/min, time 15min, obtain wet albumen, drying gets dried albumen.
3, processing waste waters such as hairtail, white Chinese croaker, mackerel, flesh of fish particle all is the loose fish protein of muscle, does not carry out enzymolysis, and protein body is filtered out, drying, pulverizing directly mix with the albumen powder that makes after sedimentation in the filtered fluid is centrifugal.
Claims (7)
1. the extracting method of the useless water fish albumen of an aquatic products processing, it is characterized in that removing earlier the lipid that floats on the waste water, subsequently the waste water of removing flesh of fish particle is carried out isoelectric point determination, pH value to the isoelectric point of regulating waste water again makes protein deposition, remove supernatant then and get protein liquid, at last the gained protein liquid is obtained wet albumen with centrifugal method.
2. extracting method as claimed in claim 1 is characterized in that after removing the lipid that floats on the waste water, will oppress particle separation with the method for filtering earlier and go out waste water.
3. extracting method as claimed in claim 1 or 2, it is characterized in that with the method for filtering filter the flesh of fish particle gained protein liquid is being added or convection drying when centrifugal.
4. extracting method as claimed in claim 2, it is characterized in that to filter with the method for filtering flesh of fish particle get albumen powder with the method for drying and crushing.
5. extracting method as claimed in claim 2, it is characterized in that to filter with the method for filtering flesh of fish particle be hydrolyzed with enzyme process, get liquid phase again and regulate the pH value and make protein deposition, remove supernatant then and get protein liquid, at last the gained protein liquid is obtained wet albumen with centrifugal method.
6. as claim 1 or 5 described extracting methods, it is characterized in that when protein deposition, adding settling agent.
7. as claim 1 or 5 described extracting methods, it is characterized in that the dry albumen powder that gets of the wet albumen of gained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100677036A CN101263859A (en) | 2007-03-16 | 2007-03-16 | Method for extracting fish protein from wastewater for processing aquatic product |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100677036A CN101263859A (en) | 2007-03-16 | 2007-03-16 | Method for extracting fish protein from wastewater for processing aquatic product |
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| CN101263859A true CN101263859A (en) | 2008-09-17 |
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| CNA2007100677036A Pending CN101263859A (en) | 2007-03-16 | 2007-03-16 | Method for extracting fish protein from wastewater for processing aquatic product |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101973675A (en) * | 2010-10-21 | 2011-02-16 | 徐文中 | Method for extracting protein from fish meal processing wastewater and making wastewater up to discharge standard |
| CN104543323A (en) * | 2015-01-13 | 2015-04-29 | 华南理工大学 | High-yield low-pollution discharge acid soluble method for extracting fish protein and minced fillet thereof |
| CN104799086A (en) * | 2015-04-24 | 2015-07-29 | 郭忠霖 | Marine product feed and processing technology thereof |
| CN107129068A (en) * | 2017-05-02 | 2017-09-05 | 上海纳德生物科技有限公司 | Recovery prepares fish oil, the method for fish protein peptide in a kind of processing waste water from minced fillet |
| CN109247424A (en) * | 2018-09-21 | 2019-01-22 | 天津农学院 | A kind of method and its application preparing modified protein by minced fish rinse water |
| CN109479772A (en) * | 2018-12-07 | 2019-03-19 | 浙江海洋大学 | Technical method of artificial propagation of rainbow trout |
| CN110003307A (en) * | 2019-04-29 | 2019-07-12 | 中国海洋大学 | A kind of method of Protein Recovery in proteinic wastewater |
-
2007
- 2007-03-16 CN CNA2007100677036A patent/CN101263859A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101973675A (en) * | 2010-10-21 | 2011-02-16 | 徐文中 | Method for extracting protein from fish meal processing wastewater and making wastewater up to discharge standard |
| CN104543323A (en) * | 2015-01-13 | 2015-04-29 | 华南理工大学 | High-yield low-pollution discharge acid soluble method for extracting fish protein and minced fillet thereof |
| CN104799086A (en) * | 2015-04-24 | 2015-07-29 | 郭忠霖 | Marine product feed and processing technology thereof |
| CN107129068A (en) * | 2017-05-02 | 2017-09-05 | 上海纳德生物科技有限公司 | Recovery prepares fish oil, the method for fish protein peptide in a kind of processing waste water from minced fillet |
| CN109247424A (en) * | 2018-09-21 | 2019-01-22 | 天津农学院 | A kind of method and its application preparing modified protein by minced fish rinse water |
| CN109479772A (en) * | 2018-12-07 | 2019-03-19 | 浙江海洋大学 | Technical method of artificial propagation of rainbow trout |
| CN109479772B (en) * | 2018-12-07 | 2021-05-11 | 浙江海洋大学 | Artificial propagation technical method for rainbow trout |
| CN110003307A (en) * | 2019-04-29 | 2019-07-12 | 中国海洋大学 | A kind of method of Protein Recovery in proteinic wastewater |
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Open date: 20080917 |