CN101843685B - BC febrifugal refreshment paste for auxiliary treatment of fever and emergent physical hypothermia and preparation thereof - Google Patents
BC febrifugal refreshment paste for auxiliary treatment of fever and emergent physical hypothermia and preparation thereof Download PDFInfo
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Abstract
The invention relates to a BC febrifugal refreshment paste for auxiliary treatment of fever and emergent physical hypothermia and preparation thereof. The BC febrifugal refreshment paste comprises the components: a bacterial cellulose membrane and febrifugal refreshment medicinal reagent. The preparation comprises the following steps of: performing seed culture on BC production strains at the temperature of 30 DEG C, inoculating the BC production strains to a fermentation culture medium for culture to prepare a BC wet membrane, then immersing the wet membrane into NaOH solution, and performing water bath until the BC membrane is white and semitransparent and putting the BC membrane aside for later use; and then putting the BC membrane into heat-clearing refreshment medicinal liquid to process the BC membrane for 5 to 30 minutes to prepare the febrifugal refreshment paste. The bacteria cellulose-based febrifugal refreshment paste has the advantages of high moisture content, good biocompatibility, no conglutination and stimulation to skin, safety and environmental protection; and the preparation method has the advantages of simplicity, convenience, easy operation, low cost and friendliness to environment, and has the excellent application prospect.
Description
Technical field
The invention belongs to bring down a fever in the biological medicine material refreshment paste and preparation field thereof, particularly a kind of BC that is used to generate heat auxiliary treatment and emergent physical cooling bring down a fever refreshment paste and preparation thereof.
Background technology
Bacterial cellulose (Bacterial Cellulose; BC) be a kind of by the synthetic native cellulose of microorganism; See from molecular formula what difference BC and common plant cellulose do not have, but give its high mechanical strength and biocompatibility because of physics, the chemical constitution of uniqueness.The BC that is shaped is made up of the fiber of highly crystalline body and highly single guiding banded superfine fibre; Be intertwined complicatedly and form unique tridimensional network; The unique tridimensional network of BC inside has a lot " duct "; High ventilation, water permeability are arranged, can absorb 60～700 times of moisture content to its dry weight, these moisture are that the form with Free water exists.Use BC can take away the heat of skin as the substrate of the refreshment paste of bringing down a fever through the evaporation of the Free water that wherein is rich in.Simultaneously cellulose not can with the skin adhesion, do not have residual when peeling off yet.
The refreshment paste of bringing down a fever of report mainly is substrate with the non-woven fabrics at present, and coating one deck high molecular weight hydrophilic gel covers one deck isolation protection film more above.Wherein contain natural refrigerant fragrant volatile plant essence oil in the hydrophilic macromolecule gel layer, this quintessence oil has the effect of heat clearing away refreshment.But find the effect and not obvious of bringing down a fever of this refreshment paste of bringing down a fever through overtesting, the contained Free water of high-molecular gel layer wherein seldom can not be taken away the heat of skin through the evaporation of moisture, and only by refrigerant sensation that quintessence oil brought.At present, do not see both at home and abroad the research and development report of the bacteria cellulose-based febrifugal refreshment paste of be used to generate heat auxiliary treatment and emergent physical cooling as yet.
Therefore the bacteria cellulose-based febrifugal refreshment paste of auxiliary treatment and emergent physical cooling of being used to generate heat has very large application prospect in the bio-medical material industry.Simplify production technology simultaneously, reduce production costs BC the applying of refreshment paste of bringing down a fever also played very big impetus.
Summary of the invention
Technical problem to be solved by this invention provides a kind of BC that is used to generate heat auxiliary treatment and emergent physical cooling bring down a fever refreshment paste and preparation thereof; This bacteria cellulose-based febrifugal refreshment paste moisture content is high; Water evaporates can effectively be taken away the human heat; Good biocompatibility, adhesion be chafe not also, safety and environmental protection; And method for preparing is easy, and is easy to operate, with low cost, environmentally friendly, has a good application prospect.
A kind of BC that is used to generate heat auxiliary treatment and emergent physical cooling of the present invention refreshment paste of bringing down a fever, its component comprises: bacteria cellulose film with bring down a fever the refreshment medicinal reagent, the bring down a fever moisture content of refreshment paste of BC is 20%～99.7%.
The described refreshment medicinal reagent of bringing down a fever, its component comprises: 1wt%～50wt% bring down a fever refreshment quintessence oil, 15wt%～40wt% ethanol, all the other are water; Wherein, the refreshment quintessence oil of bringing down a fever is selected from one or more the mixture in Herba Menthae, Borneolum Syntheticum, Camphora, cinnamic aldehyde, methoxybenzene, eugenol, eucalyptus oil, oleum bergamottae, Radix Bupleuri volatile oil, Oleum Caryophylli, the Chenopodium Oil.
The refreshment medicinal reagent of preferably bringing down a fever, its component comprises: Herba Menthae 1wt%～20wt%, Borneolum Syntheticum 2wt%～30wt%, ethanol 15wt%～40wt%, all the other are water.
The bring down a fever preparation of refreshment paste of a kind of BC that is used to generate heat auxiliary treatment and emergent physical cooling of the present invention comprises:
(1) preparation of BC film and processing
In liquid seed culture medium, seed culture is transferred in the fermentation medium after 24 hours again under 30 ℃ of conditions with BC production inoculation; 20-30 ℃ leaves standstill cultivation 1-10 days, and results Bacterial cellulose BC wet film is soaked in the NaOH solution of 0.5～2wt% then; 70-100 ℃ of water bath processing 30-120min; It is translucent to make the BC wet film become white, is cut into diaphragm again, subsequent use;
(2) preparation of heat clearing away refreshment medicinal liquid: the component of medicinal liquid comprises, the above-mentioned refreshment quintessence oil of bringing down a fever of 1wt%～50wt%, 15wt%～30wt% ethanol, and all the other are water;
(3) the BC preparation of refreshment paste of bringing down a fever
The BC film of step (1) preparation is put into heat clearing away refreshment medicinal liquid, handle after 5～30 minutes, cooling drains and promptly gets the refreshment paste of bringing down a fever.
BC film in the said step (1) is obtained through fermenting process of preparing by the microorganism of producing BC; The chemical composition of BC film comprise cellulose (as glucosan etc.) or also comprise other compositions (like cellulose derivatives such as chitosan, starch, xanthan gum, gelatin, alginate, carboxymethyl cellulose, dextrin, agar etc.), inside is nanofiber network structure.
It is acetic acid Pseudomonas (Acetobacter sp.) that BC in the said step (1) produces bacterial strain; Bacterium gluconicum belongs to (Gluconobacter sp.); Gluconic acid Acetobacter sp. (Gluconacetobacter sp.); Rhizobium (Rhizobium sp.); Sarcina (Sarcina sp.); Rhodopseudomonas (Pseudomounas sp.); Achromobacter (Achromobactersp.); Alcaligenes (Alcaligenes sp.); Aerobacter (Aerobacter sp.); Azotobacter (Azotobacter sp.); Agrobacterium (Agrobacterium sp.); Pseudomonas cepacia (Seudomonas cepacia); Campylobacter jejuni (Campylobacter jejuni) or red tea fungus (kombucha);
Wherein, the strain except that red tea fungus inserts liquid seed culture medium by the inoculum concentration of 2～3 inoculating loops, and is transferred to fermentation medium by 3～15% inoculum concentration; Red tea fungus inserts liquid seed culture medium by the inoculum concentration that inserts 1-3 sheet diameter 1cm disk Mycoderma, and is transferred to fermentation medium by the inoculum concentration of 1-3 sheet diameter 1cm disk Mycoderma.
Described except red tea fungus seed culture medium or fermentation medium; Its component is: in every 100ml water; Mannitol, glucose, sucrose or fructose 2-12g, peptone or tryptone 0.3g, yeast extract 0.5g, pH3.3-7.5,121 ℃ of sterilization 20min; Perhaps be: in every 100ml water, mannitol, glucose, sucrose or fructose 2-12g, yeast extract 0.5g, peptone or tryptone 0.5g, citric acid 0.115g, Na
2HPO
40.27g, pH3.3-7.5.
Described red tea fungus seed culture medium or fermentation medium; Its composition is: in every 100ml water, and green tea or black tea 0.5g, glucose, sucrose or fructose 1～12g, peptone or tryptone 0.3g, yeast extract 0.5g; The pH nature, pasteurization 30min; Perhaps glucose, sucrose or fructose, green tea or black tea and water are made into culture medium, wherein the mass ratio of sugar, tea, water is 5: 0.1-0.4: 100-200, pH nature, pasteurization 30min.
Processing in the said step (3) comprises adopts the method for dipping after rotary evaporation, direct impregnation, the squeezing or dry back dipping to handle.
The condition of described rotary evaporation is 40-90 ℃, vacuum-0.1MPa.
Described squeezing back dipping is meant that diaphragm puts into the medicinal liquid dipping after squeezing squeezes out part moisture;
Drying meanss such as described dry back dipping is meant that diaphragm puts into the medicinal liquid dipping after drying, and drying means comprises lyophilizing, dry, dry;
The dipping of more than mentioning comprises merceration stain or hot dipping stain, and the hot dipping stain is put into the diaphragm dipping after being meant medicinal liquid being boiled, and the merceration stain is meant that diaphragm is directly put into the medicinal liquid dipping under room temperature get final product.
The BC that the present invention the obtains refreshment paste of bringing down a fever has the light soft characteristic of quality; Can be according in the actual application; Use the situation and the characteristics at position to be cut into different shape arbitrarily; Heat clearing away refreshment reagent, medicine that those are conventional, or their compositions also may be used in the refreshment paste of bringing down a fever of the present invention.
Beneficial effect
(1) bacteria cellulose-based febrifugal refreshment paste moisture content of the present invention is high; Water evaporates can effectively be taken away the human heat; The medicine that wherein contains the heat clearing away refreshment can be alleviated incompatibility symptoms such as the headache that caused by heating, dizziness; This defervescence plaster used quality softness is light simultaneously, can use the shape at position and area to be cut into different shape according to reality;
(2) this defervescence plaster used good biocompatibility, adhesion be chafe not also, and safety and environmental protection abandons the back and in environment, can degrade rapidly, can be used as a kind of functional medical material of environmental protection;
(3) this defervescence plaster used method for preparing is easy, easy to operate, with low cost, environmentally friendly, has a good application prospect.
Description of drawings
Fig. 1 prepared identical refreshment paste and commercially available defervescence plaster used of bringing down a fever of area of BC film that different incubation times obtain of serving as reasons is measured different defervescence plaster used weight change situation at 0～6 hour under 38 ℃ of constant temperature;
Fig. 2 prepared identical refreshment paste and commercially available defervescence plaster used of bringing down a fever of area of BC film that different incubation times obtain of serving as reasons is measured different defervescence plaster used weight change situation at 0～6 hour under 39 ℃ of constant temperature;
Fig. 3 prepared identical refreshment paste and commercially available defervescence plaster used of bringing down a fever of area of BC film that different incubation times obtain of serving as reasons, at 39 ℃ of constant temperature, the relative humidity that controls environment is being measured different defervescence plaster used weight change situation at 0～5 hour for 60%～70% time;
Fig. 4 prepared identical refreshment paste and commercially available defervescence plaster used of bringing down a fever of area of BC film that different incubation times obtain of serving as reasons is measured the different defervescence plaster used thermometeies that wrap up 0～5 hour registration situation of change under 38 ℃ of constant temperature;
Fig. 5 prepared identical refreshment paste and commercially available defervescence plaster used of bringing down a fever of area of BC film that different incubation times obtain of serving as reasons is measured the different defervescence plaster used thermometeies that wrap up 0～5 hour registration situation of change under 39 ℃ of constant temperature.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
1, the preparation of BC film and processing
Acetobacter xylinum (Acetobacter xylinum) by the inoculum concentration of 2～3 inoculating loops after 30 ℃ of seed culture mediums spread cultivation 24 hours; Being transferred in the fermentation medium 30 ℃ by 6% inoculum concentration again leaves standstill respectively and cultivated results Bacterial cellulose (BC) wet film 4,7,10 days; The BC film for preparing is immersed in 1% the NaOH solution 80 ℃ of water-baths translucent until the BC film color that bleaches; With the BC film that makes and commercially availablely defervescence plaster usedly be cut into after the rectangle that diameter is 2cm * 4.5cm subsequent usely respectively, wherein the thickness of BC_4d is that the thickness of 1.61mm, BC_7d is that the thickness of 2.78mm, BC_10d is that 3.44mm, commercially available defervescence plaster used thickness are 2.50mm then;
Wherein, seed culture medium and fermentation medium are: mannitol, glucose, sucrose or fructose 2.5g, peptone or tryptone 0.3g, yeast extract 0.5g, water 100ml, pH5.0,121 ℃ of sterilization 20min;
2, the preparation of heat clearing away refreshment medicinal liquid: Herba Menthae 1wt%, Borneolum Syntheticum 2wt%, ethanol 15wt%, all the other are water;
3, the BC preparation of refreshment paste of bringing down a fever
Put into the BC diaphragm for preparing after heat clearing away refreshment medicinal liquid boiled, adopt the method preparation (being about to put into the diaphragm dipping after medicinal liquid will boil) of direct heat dipping, after 20 minutes, cooling drains and promptly gets the refreshment paste of bringing down a fever, and wherein, is 10mg/cm to the drug loading of Herba Menthae
3, be 20mg/cm to the drug loading of Borneolum Syntheticum
3
4, the bring down a fever effect of refreshment paste of the BC for preparing adopts following method to estimate
The cultivation that area is identical the prepared refreshment paste and commercially available defervescence plaster used of bringing down a fever of BC film that obtained in 4,7,10 days, (ambient humidity about 20%) measured different defervescence plaster used weight change situation at 0～6 hour under 38 ℃ of constant temperature.
Experimental result is seen Fig. 1; The result is illustrated under 38 ℃; The various defervescence plaster used moisture loss rates of 2h are all bigger before the experiment; Wherein commercially available defervescence plaster used moisture loss rate is 0.31g/h, and incubation time is that 4,7 and 10 days defervescence plaster used scatter ratio, scatter and disappear ratios of prepared BC then are respectively 0.41,0.37 and 0.61g/h, all is higher than commercially available defervescence plaster used.In 6h; The average scatter ratio, scatter and disappear ratio of commercially available defervescence plaster used moisture then is 0.17g/h; Incubation time is that 4,7 and 10 days defervescence plaster used average scatter ratio, scatter and disappear ratios of moisture of prepared BC then are respectively 0.18,0.35,0.46g/h, is commercially available defervescence plaster used 1.06,2.06 and 2.71 times.
Conclusion: in the time of 38 ℃, the BC refreshment paste of bringing down a fever causes the loss in weight defervescence plaster used bigger than commercially available because of water evaporates, and the heat that more water evaporates is taken away skin is posted in this explanation BC refreshment of bringing down a fever, and plays the effect of better bringing down a fever; And the defervescence plaster used moisture loss rate of BC reduces along with the increase of the incubation time of BC film, just along with the increase of the thickness of BC film and reduce.
1, the preparation of BC film and processing
Gluconic acid acetobacter (Gluconacetobacter xylinus) by the inoculum concentration of 2～3 inoculating loops after 30 ℃ of seed culture mediums spread cultivation 24 hours; Being transferred in the fermentation medium 30 ℃ by 6% inoculum concentration again leaves standstill respectively and cultivated results Bacterial cellulose (BC) wet film 4,7,10 days; The BC film for preparing is immersed in 2% the NaOH solution 90 ℃ of water-baths translucent until the BC film color that bleaches; With the BC film that makes and commercially availablely defervescence plaster usedly be cut into after the rectangle that diameter is 2cm * 4.5cm subsequent usely, wherein the thickness of BC_4d is that the thickness of 1.62mm, BC_7d is that the thickness of 2.74mm, BC_10d is that 3.47mm, commercially available defervescence plaster used thickness are 2.50mm then;
Wherein, seed culture medium and fermentation medium are: mannitol, glucose, sucrose or fructose 10g, yeast extract 0.5g, peptone 0.5g, citric acid 0.115g, Na
2HPO
40.27g, water 100ml, pH3.3,121 ℃ of sterilization 20min;
2, the preparation of heat clearing away refreshment medicinal liquid: Herba Menthae 4wt%, Borneolum Syntheticum 8wt%, ethanol 15wt%, all the other are water;
3, the BC preparation of refreshment paste of bringing down a fever
Put into the BC diaphragm for preparing after heat clearing away refreshment medicinal liquid boiled; Adopt the method preparation (being about to put into medicinal liquid merceration stain diaphragm squeezes out 10～20% moisture through squeezing after) of squeezing back dipping, after 30 minutes, cooling drains and promptly gets the refreshment paste of bringing down a fever; Wherein, the drug loading to Herba Menthae is 40mg/cm
3, be 80mg/cm to the drug loading of Borneolum Syntheticum
3
4, the bring down a fever effect of refreshment paste of the BC for preparing adopts following method to estimate
The cultivation that area is identical the prepared refreshment paste and commercially available defervescence plaster used of bringing down a fever of BC film that obtained in 4,7,10 days, (ambient humidity about 20%) measured different defervescence plaster used weight change situation at 0～6 hour under 39 ℃ of constant temperature.
Experimental result is seen Fig. 2; The result is illustrated under 39 ℃; The various defervescence plaster used moisture loss rates of 2h are all bigger before the experiment, and wherein commercially available defervescence plaster used moisture loss rate is 0.42g/h, and incubation time is that 4,7 and 10 days defervescence plaster used scatter ratio, scatter and disappear ratios of prepared BC then are respectively 0.44,0.53,0.57g/h.In 6h, the average scatter ratio, scatter and disappear ratio of commercially available defervescence plaster used moisture then is 0.20g/h, and incubation time is that 4,7 and 10 days defervescence plaster used average scatter ratio, scatter and disappear ratios of moisture of prepared BC then are respectively 0.20,0.31,0.43g/h, is commercially available defervescence plaster used 1,1.55 and 2.15 times.
Conclusion: in the time of 39 ℃, the BC refreshment paste of bringing down a fever causes the loss in weight defervescence plaster used bigger than commercially available because of water evaporates, and the heat that more water evaporates is taken away skin is posted in this explanation BC refreshment of bringing down a fever, and plays the effect of better bringing down a fever; And the defervescence plaster used moisture loss rate of BC reduces along with the increase of the incubation time of BC film, just along with the increase of the thickness of BC film and reduce.
1, the preparation of BC film and processing
Bacterium gluconicum (Gluconobacter xylinum) by the inoculum concentration of 2～3 inoculating loops after 30 ℃ of seed culture mediums spread cultivation 24 hours; Being transferred in the fermentation medium 30 ℃ by 6% inoculum concentration again leaves standstill respectively and cultivated results Bacterial cellulose (BC) wet film 4,7,10 days; The BC film for preparing is immersed in 0.5% the NaOH solution 100 ℃ of water-baths translucent until the BC film color that bleaches; With the BC film that makes and commercially availablely defervescence plaster usedly be cut into after the rectangle that diameter is 2cm * 4.5cm subsequent usely, wherein the thickness of BC_4d is that the thickness of 1.64mm, BC_7d is that the thickness of 2.68mm, BC_10d is that 3.38mm, commercially available defervescence plaster used thickness are 2.50mm then;
Wherein, seed culture medium and fermentation medium are: mannitol, glucose, sucrose or fructose 2g, yeast extract 0.5g, peptone 0.5g, citric acid 0.115g, Na
2HPO
40.27g, water 100ml, pH7.5,121 ℃ of sterilization 20min;
2, the preparation of heat clearing away refreshment medicinal liquid: Herba Menthae 6wt%, Borneolum Syntheticum 12wt%, ethanol 30wt%, all the other are water;
3, the BC preparation of refreshment paste of bringing down a fever
With BC film for preparing and heat clearing away refreshment medicinal liquid, put into flask, connect Rotary Evaporators, rotary evaporation is after 15 minutes under the condition of 40-90 ℃ and vacuum-0.1MPa, and cooling drains and promptly gets the refreshment paste of bringing down a fever, and wherein, is 60mg/cm to the drug loading of Herba Menthae
3, be 120mg/cm to the drug loading of Borneolum Syntheticum
3
4, the bring down a fever effect of refreshment paste of the BC for preparing adopts following method to estimate
The cultivation that area is identical the prepared refreshment paste and commercially available defervescence plaster used of bringing down a fever of BC film that obtained in 4,7,10 days, at 39 ℃ of constant temperature, envionmental humidity is controlled at the different defervescence plaster used weight change situation at 0～5 hour of measuring for 60%～70% time.
Experimental result is seen Fig. 3.It is 39 ℃ that the result is illustrated in temperature, envionmental humidity 60%～70% time, and various defervescence plaster used moisture loss rates are all smaller, but the defervescence plaster used moisture loss rate of BC is still defervescence plaster used big than commercially available.Wherein commercially available defervescence plaster used moisture loss rate is 0.05g/h, and incubation time is that 4,7 and 10 days defervescence plaster used scatter ratio, scatter and disappear ratios of prepared BC then are respectively 0.12,0.12 and 0.15g/h, is commercially available defervescence plaster used 2.4,2.4 and 3 times.
Conclusion: at 39 ℃; When envionmental humidity is higher; These several kinds defervescence plaster used moisture loss situation are not very big, and the moisture loss rate of refreshment paste is all defervescence plaster used big than commercially available but the BC of different size brings down a fever, and this explains under the condition of high ambient humidity; The Free water that BC brings down a fever in the refreshment paste is evaporated the heat of taking away skin more easily, and plays the effect of better bringing down a fever; And the defervescence plaster used moisture loss rate of BC reduces along with the increase of the incubation time of BC film, just along with the increase of the thickness of BC film and reduce.
1, the preparation of BC film and processing
Acetobacter xylinum (Acetobacter xylinum) by the inoculum concentration of 2～3 inoculating loops after 30 ℃ of seed culture mediums spread cultivation 24 hours; Being transferred in the fermentation medium 30 ℃ by 6% inoculum concentration again leaves standstill respectively and cultivated results Bacterial cellulose (BC) wet film 4,7,10 days; The BC film for preparing is immersed in 0.5% the NaOH solution 100 ℃ of water-baths translucent until the BC film color that bleaches; Defervescence plaster used be sure to into rectangle that diameter be 1cm * 3cm after subsequent use with commercially available the BC film that makes then, wherein the thickness of BC_4d is that the thickness of 1.61mm, BC_7d is that the thickness of 2.78mm, BC_10d is that 3.44mm, commercially available defervescence plaster used thickness are 2.50mm;
Wherein, seed culture medium and fermentation medium are: mannitol, glucose, sucrose or fructose 6g, peptone or tryptone 0.3g, yeast extract 0.5g, water 100ml, pH5.5,121 ℃ of sterilization 20min;
2, the preparation of heat clearing away refreshment medicinal liquid: Herba Menthae 10wt%, Borneolum Syntheticum 20wt%, ethanol 30wt%, all the other are water;
3, the BC preparation of refreshment paste of bringing down a fever
With BC film for preparing and heat clearing away refreshment medicinal liquid, adopt the method (be about to diaphragm and after drying, put into medicinal liquid hot dipping stain) dry the back dipping, handles after 25 minutes, cool off to drain and promptly get the refreshment paste of bringing down a fever, wherein, be 100mg/cm to the drug loading of Herba Menthae
3, be 200mg/cm to the drug loading of Borneolum Syntheticum
3
4, the bring down a fever effect of refreshment paste of the BC for preparing adopts following method to estimate
The prepared refreshment paste and commercially available defervescence plaster used of bringing down a fever of BC film that obtains by different incubation times that area is identical; Be wrapped on the vacuole of mercurial thermometer; Be positioned in the calorstat then; At 38 ℃, measure the different defervescence plaster used thermometeies that wrap up under about 19% condition of ambient humidity 0～5 hour registration situation of change.
Experimental result is seen Fig. 4; The result is illustrated under 38 ℃; The commercially available defervescence plaster used thermometer registration rate of change that wraps up 2h before experiment changes greatly, and its rate of change is 8.75 ℃/h, and incubation time is that 4,7 and 10 days defervescence plaster used thermometer registration rates of change that wrap up of prepared BC are 4 ℃/h, 4.25 ℃/h and 2.5 ℃/h; Obviously slow than commercially available defervescence plaster used intensification, its rate of change is respectively 6.33 ℃/h, 6 ℃/h, 6 ℃/h in the 3h before experiment.
Conclusion: in the time of 38 ℃, be wrapped in BC and bring down a fever that to compare the commercially available defervescence plaster used rising of parcel slow for the thermometer registration of refreshment paste, this explanation BC refreshment of bringing down a fever is posted more water evaporates and is taken away heat, and plays the effect of better bringing down a fever; And be wrapped in by the thermometer registration of cultivating 10 days the made refreshment paste of bringing down a fever of BC film rise the slowest.
1, the preparation of BC film and processing
Acetobacter xylinum (Acetobacter xylinum) by the inoculum concentration of 2～3 inoculating loops after 30 ℃ of seed culture mediums spread cultivation 24 hours; Being transferred in the fermentation medium 30 ℃ by 6% inoculum concentration again leaves standstill respectively and cultivated results Bacterial cellulose (BC) wet film 4,7,10 days; The BC film for preparing is immersed in 0.5% the NaOH solution 100 ℃ of water-baths translucent until the BC film color that bleaches; With the BC film that makes and commercially availablely defervescence plaster usedly be cut into after the rectangle that diameter is 2cm * 4.5cm subsequent usely, wherein the thickness of BC_4d is that the thickness of 1.61mm, BC_7d is that the thickness of 2.78mm, BC_10d is that 3.44mm, commercially available defervescence plaster used thickness are 2.50mm then;
Wherein, seed culture medium and fermentation medium are: mannitol, glucose, sucrose or fructose 12g, peptone or tryptone 0.3g, yeast extract 0.5g, water 100ml, pH6.0,121 ℃ of sterilization 20min;
2, the preparation of heat clearing away refreshment medicinal liquid: Herba Menthae 20wt%, Borneolum Syntheticum 30wt%, ethanol 40wt%, all the other are water;
3, the BC preparation of refreshment paste of bringing down a fever
The BC film for preparing after the freeze drier lyophilizing, at room temperature being impregnated in the heat clearing away refreshment medicinal liquid, drain behind the 1-24h and promptly get the refreshment paste of bringing down a fever, wherein, is 200mg/cm to the drug loading of Herba Menthae
3, be 300mg/cm to the drug loading of Borneolum Syntheticum
3
4, the bring down a fever effect of refreshment paste of the BC for preparing adopts following method to estimate
The prepared refreshment paste and commercially available defervescence plaster used of bringing down a fever of BC film that is obtained by different incubation times that area is identical is wrapped on the vacuole of mercurial thermometer.Be positioned over then in the calorstat,, measure the different defervescence plaster used thermometeies that wrap up under about 20% condition of ambient humidity 0～5 hour registration situation of change at 39 ℃.
Experimental result is seen Fig. 5, and the result is illustrated under 39 ℃, and the various defervescence plaster used thermometer registration rates of change that wrap up 3h before experiment changes greatly.The commercially available defervescence plaster used thermometer registration rate of change that wraps up is 4.6 ℃/h in the 1h, and incubation time is that 4,7,10 days defervescence plaster used thermometer registration rates of change that wrap up of prepared BC then are respectively 4.7 ℃/h, 4 ℃/h and 3 ℃/h; The commercially available defervescence plaster used thermometer registration rate of change that wraps up is 7.25 ℃/h in the 2h, and incubation time is that 4,7,10 days defervescence plaster used thermometer registration rates of change that wrap up of prepared BC then are respectively 6.5 ℃/h, 6.5 ℃/h and 5.5 ℃/h.All explain of the present invention defervescence plaster used can be than the commercially available defervescence plaster used more frequent fever amount of taking away; In the 3h, the commercially available defervescence plaster used thermometer registration average rate of change of wrapping up is 5.5 ℃/h, and incubation time is that 4,7,10 days defervescence plaster used thermometer registration average rate of change of wrapping up of prepared BC then is respectively 6 ℃/h, 6.5 ℃/h, 6 ℃/h.This is after scattering and disappearing owing to the moisture in defervescence plaster used is a large amount of, can not walk heat through the evaporator strip of moisture again, thereby delay the rising of temperature, and this moment, defervescence plaster used internal and external environment temperature was almost consistent, no longer there are differences.
Conclusion: in the time of 39 ℃, be wrapped in BC and bring down a fever that to compare the commercially available defervescence plaster used rising of parcel slow for the thermometer registration of refreshment paste, this explanation BC refreshment of bringing down a fever is posted more water evaporates and is taken away heat, and plays the effect of better bringing down a fever; And be wrapped in by the thermometer registration of cultivating 10 days the made refreshment paste of bringing down a fever of BC film rise the slowest.But in the time of 39 ℃, this difference neither be very big.
1, the preparation of BC film and processing
Red tea fungus (kombucha) by the inoculum concentration that inserts 1-3 sheet diameter 1cm disk Mycoderma in leave standstill under 30 ℃ of the seed culture mediums spread cultivation 2-4 days after; Being transferred in the fermentation medium 30 ℃ by the inoculum concentration that inserts 1-3 sheet diameter 1cm disk Mycoderma again leaves standstill respectively and cultivated results Bacterial cellulose (BC) wet film 4,7,10 days; The BC film for preparing is immersed in 0.5% the NaOH solution 100 ℃ of water-baths translucent until the BC film color that bleaches; With the BC film that makes and commercially availablely defervescence plaster usedly be cut into after the rectangle that diameter is 2cm * 4.5cm subsequent usely, wherein the thickness of BC_4d is that the thickness of 1.95mm, BC_7d is that the thickness of 2.98mm, BC_10d is that 3.84mm, commercially available defervescence plaster used thickness are 2.50mm then;
Wherein, seed culture medium and fermentation medium are: water 100ml boils, and adds green tea 0.5g bubble and opens 20min; Filtered through gauze gets millet paste; Add glucose, sucrose or fructose 12g, peptone or tryptone 0.3g, yeast extract 0.5g, pH nature, pasteurization 30min;
Perhaps with water glucose, sucrose or fructose and black tea or green tea water are made into culture medium, the mass ratio of sugar, tea, water is 5:0.2:150, pH nature, pasteurization 30min;
2, the preparation of heat clearing away refreshment medicinal liquid: Herba Menthae 20wt%, Borneolum Syntheticum 30wt%, ethanol 40wt%, all the other are water;
3, the BC preparation of refreshment paste of bringing down a fever
The BC film for preparing after the freeze drier lyophilizing, at room temperature being impregnated in the heat clearing away refreshment medicinal liquid, drain behind the 1-24h and promptly get the refreshment paste of bringing down a fever, wherein, is 200mg/cm to the drug loading of Herba Menthae
3, be 300mg/cm to the drug loading of Borneolum Syntheticum
3
4, the bring down a fever effect of refreshment paste of the BC for preparing adopts following method to estimate
The prepared refreshment paste and commercially available defervescence plaster used of bringing down a fever of BC film that is obtained by different incubation times that area is identical is wrapped on the vacuole of mercurial thermometer.Be positioned over then in the calorstat,, measure the different defervescence plaster used thermometeies that wrap up under about 20% condition of ambient humidity 0～5 hour registration situation of change at 39 ℃.
Experimental result is seen Fig. 5, and the result is illustrated under 39 ℃, and the various defervescence plaster used thermometer registration rates of change that wrap up 3h before experiment changes greatly.The commercially available defervescence plaster used thermometer registration rate of change that wraps up is 4.6 ℃/h in the 1h, and incubation time is that 4,7,10 days defervescence plaster used thermometer registration rates of change that wrap up of prepared BC then are respectively 4.7 ℃/h, 4 ℃/h and 3 ℃/h; The commercially available defervescence plaster used thermometer registration rate of change that wraps up is 7.25 ℃/h in the 2h, and incubation time is that 4,7,10 days defervescence plaster used thermometer registration rates of change that wrap up of prepared BC then are respectively 6.5 ℃/h, 6.5 ℃/h and 5.5 ℃/h.All explain of the present invention defervescence plaster used can be than the commercially available defervescence plaster used more frequent fever amount of taking away; In the 3h, the commercially available defervescence plaster used thermometer registration average rate of change of wrapping up is 5.5 ℃/h, and incubation time is that 4,7,10 days defervescence plaster used thermometer registration average rate of change of wrapping up of prepared BC then is respectively 6 ℃/h, 6.5 ℃/h, 6 ℃/h.This is after scattering and disappearing owing to the moisture in defervescence plaster used is a large amount of, can not walk heat through the evaporator strip of moisture again, thereby delay the rising of temperature, and this moment, defervescence plaster used internal and external environment temperature was almost consistent, no longer there are differences.
Conclusion: in the time of 39 ℃, be wrapped in BC and bring down a fever that to compare the commercially available defervescence plaster used rising of parcel slow for the thermometer registration of refreshment paste, this explanation BC refreshment of bringing down a fever is posted more water evaporates and is taken away heat, and plays the effect of better bringing down a fever; And be wrapped in by the thermometer registration of cultivating 10 days the made refreshment paste of bringing down a fever of BC film rise the slowest.But in the time of 39 ℃, this difference neither be very big.
The component of heat clearing away refreshment medicinal liquid is: Camphora 10wt%, eugenol 15wt%, oleum bergamottae 5wt%, Chenopodium Oil 20wt%, and ethanol 30wt%, all the other are water; Other conditions are identical with embodiment 1, are prepared into the refreshment paste of bringing down a fever.
The cultivation that area is identical the prepared refreshment paste and commercially available defervescence plaster used of bringing down a fever of BC film that obtained in 4,7,10 days, (ambient humidity about 20%) measured different defervescence plaster used weight change situation at 0～6 hour under 38 ℃ of constant temperature.Experimental result and Fig. 1 are similar.The result is illustrated under 38 ℃, and the BC refreshment paste of bringing down a fever causes the loss in weight all defervescence plaster used big than commercially available because of water evaporates, and the heat that more water evaporates is taken away skin is posted in this explanation BC refreshment of bringing down a fever, and plays the effect of better bringing down a fever.
Embodiment 8
The component of heat clearing away refreshment medicinal liquid is: cinnamic aldehyde 10wt%, methoxybenzene 10wt%, eucalyptus oil 10wt%, Radix Bupleuri volatile oil 10wt%, Oleum Caryophylli 10wt%, ethanol 20wt%, and all the other are water; Other conditions are identical with embodiment 1, are prepared into the refreshment paste of bringing down a fever.
The cultivation that area is identical the prepared refreshment paste and commercially available defervescence plaster used of bringing down a fever of BC film that obtained in 4,7,10 days, (ambient humidity about 20%) measured different defervescence plaster used weight change situation at 0～6 hour under 38 ℃ of constant temperature.Experimental result and Fig. 1 are similar.The result is illustrated under 38 ℃, and the BC refreshment paste of bringing down a fever causes the loss in weight all defervescence plaster used big than commercially available because of water evaporates, and the heat that more water evaporates is taken away skin is posted in this explanation BC refreshment of bringing down a fever, and plays the effect of better bringing down a fever; And the defervescence plaster used moisture loss rate of BC is to reduce along with the increase of BC film thickness.
Embodiment 9
The component of heat clearing away refreshment medicinal liquid is: cinnamic aldehyde 2wt%, eugenol 1wt%, eucalyptus oil 1wt%, Radix Bupleuri volatile oil 1wt%, and ethanol 30wt%, all the other are water; Other conditions are identical with embodiment 3, are prepared into the refreshment paste of bringing down a fever.
The cultivation that area is identical the prepared refreshment paste and commercially available defervescence plaster used of bringing down a fever of BC film that obtained in 4,7,10 days, at 39 ℃ of constant temperature, envionmental humidity is controlled at the different defervescence plaster used weight change situation at 0～5 hour of measuring for 60%～70% time.Experimental result and Fig. 3 are similar.It is 39 ℃ that the result is illustrated in temperature; Envionmental humidity 60%～70% time; Various defervescence plaster used moisture loss rates are all smaller, and the moisture loss rate of refreshment paste is all defervescence plaster used big than commercially available but the BC of different size brings down a fever, and this explains under the condition of high ambient humidity; The Free water that BC brings down a fever in the refreshment paste is evaporated the heat of taking away skin more easily, and plays the effect of better bringing down a fever.
The component of heat clearing away refreshment medicinal liquid is: Camphora 30wt%, and ethanol 30wt%, all the other are water; Other conditions are identical with embodiment 3, are prepared into the refreshment paste of bringing down a fever.
The cultivation that area is identical the prepared refreshment paste and commercially available defervescence plaster used of bringing down a fever of BC film that obtained in 4,7,10 days, at 39 ℃ of constant temperature, envionmental humidity is controlled at the different defervescence plaster used weight change situation at 0～5 hour of measuring for 60%～70% time.Experimental result and Fig. 3 are similar.It is 39 ℃ that the result is illustrated in temperature; Envionmental humidity 60%～70% time; Various defervescence plaster used moisture loss rates are all smaller, and the moisture loss rate of refreshment paste is all defervescence plaster used big than commercially available but the BC of different size brings down a fever, and this explains under the condition of high ambient humidity; The Free water that BC brings down a fever in the refreshment paste is evaporated the heat of taking away skin more easily, and plays the effect of better bringing down a fever; And the defervescence plaster used moisture loss rate of BC reduces along with the increase of the incubation time of BC film, just reduces along with the increase of BC film thickness.
Claims (8)
1. the BC of be used to generate heat auxiliary treatment and the emergent physical cooling refreshment paste of bringing down a fever, its component comprises: bacteria cellulose film with bring down a fever the refreshment medicinal reagent, the bring down a fever moisture content of refreshment paste of BC is 20%～99.7%; The described refreshment medicinal reagent of bringing down a fever, its component comprises: 1wt%～50wt% bring down a fever refreshment quintessence oil, 15wt%～40wt% ethanol, all the other are water; Wherein, the refreshment quintessence oil of bringing down a fever is selected from one or more the mixture in Herba Menthae, Borneolum Syntheticum, Camphora, cinnamic aldehyde, methoxybenzene, eugenol, eucalyptus oil, oleum bergamottae, Radix Bupleuri volatile oil, Oleum Caryophylli, the Chenopodium Oil;
The bring down a fever preparation of refreshment paste of the described BC that is used to generate heat auxiliary treatment and emergent physical cooling comprises:
(1) preparation of BC film and processing
In liquid seed culture medium, seed culture is transferred in the fermentation medium after 24 hours again under 30 ℃ of conditions with BC production inoculation; 20-30 ℃ leaves standstill cultivation 1-10 days, and results Bacterial cellulose BC wet film is soaked in the NaOH solution of 0.5～2wt% then; 70-100 ℃ of water bath processing 30-120min; It is translucent to make the BC wet film become white, is cut into diaphragm again, subsequent use;
(2) preparation of heat clearing away refreshment medicinal liquid: the component of medicinal liquid comprises, the above-mentioned refreshment quintessence oil of bringing down a fever of 1wt%～50wt%, 15wt%～40wt% ethanol, and all the other are water;
(3) the BC preparation of refreshment paste of bringing down a fever
The BC film of step (1) preparation is put into heat clearing away refreshment medicinal liquid, handle after 5～30 minutes, cooling drains and promptly gets the refreshment paste of bringing down a fever.
2. a kind of BC that is used to generate heat auxiliary treatment and emergent physical cooling according to claim 1 refreshment paste of bringing down a fever; It is characterized in that: the described refreshment medicinal reagent of bringing down a fever; Its component comprises: Herba Menthae 1wt%～20wt%; Borneolum Syntheticum 2wt%～30wt%, ethanol 15wt%～40wt%, all the other are water.
3. the BC of be used to generate heat auxiliary treatment and the emergent physical cooling preparation of refreshment paste of bringing down a fever comprises:
(1) preparation of BC film and processing
In liquid seed culture medium, seed culture is transferred in the fermentation medium after 24 hours again under 30 ℃ of conditions with BC production inoculation; 20-30 ℃ leaves standstill cultivation 1-10 days, and results Bacterial cellulose BC wet film is soaked in the NaOH solution of 0.5～2wt% then; 70-100 ℃ of water bath processing 30-120min; It is translucent to make the BC wet film become white, is cut into diaphragm again, subsequent use;
(2) preparation of heat clearing away refreshment medicinal liquid: the component of medicinal liquid comprises, the above-mentioned refreshment quintessence oil of bringing down a fever of 1wt%～50wt%, 15wt%～40wt% ethanol, and all the other are water;
(3) the BC preparation of refreshment paste of bringing down a fever
The BC film of step (1) preparation is put into heat clearing away refreshment medicinal liquid, handle after 5～30 minutes, cooling drains and promptly gets the refreshment paste of bringing down a fever.
4. the bring down a fever preparation of refreshment paste of a kind of BC that is used to generate heat auxiliary treatment and emergent physical cooling according to claim 3, it is characterized in that: it is acetic acid Pseudomonas, Bacterium gluconicum genus, gluconic acid Acetobacter sp., rhizobium, Sarcina, Rhodopseudomonas, achromobacter, Alcaligenes, Aerobacter, azotobacter, Agrobacterium, Pseudomonas cepacia, campylobacter jejuni or red tea fungus that described BC produces bacterial strain; Wherein, the strain except that red tea fungus inserts liquid seed culture medium by the inoculum concentration of 2～3 inoculating loops, and is transferred to fermentation medium by 3～15% inoculum concentration; Red tea fungus inserts liquid seed culture medium by the inoculum concentration that inserts 1-3 sheet diameter 1cm disk Mycoderma, and is transferred to fermentation medium by the inoculum concentration of 1-3 sheet diameter 1cm disk Mycoderma.
5. according to the bring down a fever preparation of refreshment paste of claim 3 or 4 described a kind of BC that are used to generate heat auxiliary treatment and emergent physical cooling; It is characterized in that: described except red tea fungus seed culture medium or fermentation medium; Its component is: in every 100ml water; Mannitol, glucose, sucrose or fructose 2-12g, peptone or tryptone 0.3g, yeast extract 0.5g, pH3.3-7.5,121 ℃ of sterilization 20min; Perhaps be: in every 100ml water, mannitol, glucose, sucrose or fructose 2-12g, yeast extract 0.5g, peptone or tryptone 0.5g, citric acid 0.115g, Na
2HPO
40.27g, pH3.3-7.5.
6. according to the bring down a fever preparation of refreshment paste of claim 3 or 4 described a kind of BC that are used to generate heat auxiliary treatment and emergent physical cooling; It is characterized in that: described red tea fungus seed culture medium or fermentation medium; Its composition is: in every 100ml water; Green tea or black tea 0.5g, glucose, sucrose or fructose 1～12g, peptone or tryptone 0.3g, yeast extract 0.5g, pasteurization 30min; Perhaps glucose, sucrose or fructose, green tea or black tea and water are made into culture medium, wherein the mass ratio of sugar, tea, water is 5: 0.1-0.4: 100-200, pasteurization 30min.
7. the bring down a fever preparation of refreshment paste of a kind of BC that is used to generate heat auxiliary treatment and emergent physical cooling according to claim 3 is characterized in that: the processing in the said step (3) comprises adopts the method for dipping after rotary evaporation, direct impregnation, the squeezing or dry back dipping to handle.
8. the bring down a fever preparation of refreshment paste of a kind of BC that is used to generate heat auxiliary treatment and emergent physical cooling according to claim 7, it is characterized in that: the condition of described rotary evaporation is 40-90 ℃, vacuum-0.1MPa.
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| CN104688880A (en) * | 2015-03-12 | 2015-06-10 | 施敬东 | Headache-relieving pad and preparation method thereof |
| CN104758350A (en) * | 2015-03-12 | 2015-07-08 | 施敬东 | Refreshing and cool patch and preparation method thereof |
| CN104688408B (en) * | 2015-03-12 | 2016-08-31 | 施敬东 | Medical defervescence plaster used and preparation method thereof |
| CN107698805A (en) * | 2016-08-09 | 2018-02-16 | 丁玉琴 | A kind of defervescence plaster used preparation method with compound water congealing glued membrane |
| CN106377739A (en) * | 2016-08-29 | 2017-02-08 | 魏晓兵 | Externally-applied traditional Chinese medicine for treating child high fever |
| CN110237122A (en) * | 2019-06-17 | 2019-09-17 | 雷春明 | A kind of external drug and its preparation method and application |
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