CN101843642A - Supercritical extraction technology for mushroom bacteria - Google Patents
Supercritical extraction technology for mushroom bacteria Download PDFInfo
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- CN101843642A CN101843642A CN200910119321A CN200910119321A CN101843642A CN 101843642 A CN101843642 A CN 101843642A CN 200910119321 A CN200910119321 A CN 200910119321A CN 200910119321 A CN200910119321 A CN 200910119321A CN 101843642 A CN101843642 A CN 101843642A
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- supercritical extraction
- mushroom
- thalline
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- antrodia camphorata
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Abstract
The invention relates to a supercritical extraction technology for mushroom bacteria. The mushroom bacteria are prepared by the following steps of: obtaining Extraction liquid containing a great quantity of non-polar substances by supercritical extraction; growing selected mushroom bacteria in a fermentation growth mode to reach an optimal time for generating the non-polar substances; then, taking down the mushroom bacteria from a culture medium and drying bacterium bodies to supercritically extract and obtain extraction liquid containing more non-polar substances.
Description
Technical field
The present invention mainly grows to the mushroom bacterium that apolar substance generates Best Times with postcritical extraction mode with fermentation relevant for a kind of supercritical extraction technology for mushroom bacteria, extracts to contain comparatively a large amount of apolar substance extracts.
Background technology
The extraction thing (decoction) of natural goods Chinese herbal medicine is because of having the advantage fast, that curative effect is rapid that absorbs for oral administration, so the most common in clinical practice.Since the progress of modern science, the Application and Development of some new techniques, new method, New Set composition, novel form etc., and decoction is gradually replaced by the science Chinese medicine preparation.And the science Chinese medicine preparation, in the pharmaceutical factory of traditional Chinese medicine of TaiWan, China, mostly be that the medical mushroom bacterium is added water (also adding a small amount of wine sometimes), and with behind the steam hot digestion (extraction), juice has removed slag, absorb by excipient again after concentrating, and other procedure of processing, to make the dosage form that is convenient for carrying and takes.But according to 1976, the Japan scholar extracts experiment in BANXIA HOUPU TANG and shows, oiliness component content in actual decoct has only 3.5%, and still there is oiliness composition 49.8% in the residue, all the other have 46.7% to vapor away in decocting process, so how to extract composition contained in the medical mushroom bacterium effectively, also be that of natural goods Chinese herbal medicine processing procedure research and development is big crucial.
Summary of the invention
Main purpose of the present invention is to provide a kind of supercritical extraction technology for mushroom bacteria, grows to apolar substance for the mushroom bacterium in fermentation and generates Best Times, can obtain by supercritical extraction to contain comparatively a large amount of apolar substance extracts.
Be the purpose of taking off before reaching, supercritical extraction technology for mushroom bacteria of the present invention mainly allows the mushroom bacterium grow to apolar substance in fermentation and generates Best Times, and can obtain containing more apolar substance extract by the action of supercritical extraction, will be via the mode of the selected mushroom bacterium of bacterial screening by the fermentation growth, allow mushroom cultivating under 20 ℃～35 ℃ temperature, reach apolar substance through 72 hours～144 hours skies and generate Best Times, again the mushroom bacterium is taken off on culture medium, again the mushroom bacterium is carried out the thalline drying and use and obtain the apolar substance extract for supercritical extraction.
Supercritical extraction technology for mushroom bacteria of the present invention, be particularly suitable for the Antrodia camphorata thalline or the domestication after the Antrodia camphorata sporophore.
Supercritical extraction technology for mushroom bacteria of the present invention, the mode that mainly allows the mushroom bacterium grow by fermentation grows to the apolar substance optimum extraction time, via supercritical extraction technique, can extract more apolar substance extract.
Description of drawings
Fig. 1: the present invention's schematic flow sheet;
Fig. 2: the present invention's Antrodia camphorata polysaccharides is grown up and is contained scale.
The specific embodiment
Present invention is directed to a kind of supercritical extraction technology for mushroom bacteria [seeing also Fig. 1] is example with the Antrodia camphorata thalline, and its mushroom bacterium extraction is in proper order:
Thalline drying 3 with growing to the mushroom bacterium that apolar substance generates Best Times, is cultivated and was taken off on culture medium in about 72 hours～144 hours, and carry out cold drying between 25 ℃～35 ℃.
Apolar substance extract 5 contains the mushroom bacterium liquid of a large amount of apolar substances via the extraction mode gained of supercritical extraction 5.
Use when implementing supercritical extraction technology for mushroom bacteria, [seeing also Fig. 1] at first carries out thalline screening 1 with the mushroom bacterium of desire extraction, and will be arranged on the growth 2 of fermenting of mushroom bacterium on the culture medium, allow the mushroom bacterium cultivate down and reached the best rise time in about 72 hours～144 hours in 20 ℃～35 ℃, allow the apolar substance of mushroom bacterium reach nonpolar generation Best Times 21, again the mushroom bacterium is taken off on culture medium, and the mushroom bacterium that takes off is done the action of thalline drying 3, then the temperature of thalline drying 3 is 25 ℃～35 ℃, to surpass 74atm through mushroom bacterium after the thalline drying 3 and pressurization and carry out supercritical extraction 4 with the carbon dioxide abstraction agent that is lower than 31 ℃, and contain a large amount of apolar substance extracts 5 after can obtaining supercritical extraction 4, and apolar substance extract 5 can be carried out further purification or extract required material.
Antrodia camphorata thalline apolar substance in its [seeing also Fig. 2] mushroom bacterium generates Best Times 21, apolar substance generates Best Times 21 and cultivates about 72 hours～144 hours of generation down for 20 ℃～35 ℃ in temperature, the contained Biomass of glucose of the Antrodia camphorata thalline in this time and the content of exopolysaccharide are higher, can make the Antrodia camphorata thalline obtain containing the extract of a large amount of glucoses and exopolysaccharide by supercritical extraction technique, it is also applicable at the Antrodia camphorata sporophore or on the Antrodia camphorata sporophore after the domestication that this apolar substance generates Best Times 21, about 180 days～480 days of its sporophore growth, and outward appearance is the gill fungus shape, the contained crude fat amount of Antrodia camphorata sporophore this moment is higher, and then can contain the extract of a large amount of crude fat by supercritical extraction technique.
Supercritical extraction technology for mushroom bacteria of the present invention, its advantage is, to use the mode of fermentation growth 2 through the mushroom bacterium of bacterial screening 1, allow the mushroom bacterium under 20 ℃～35 ℃ temperature, grow 72 hours～144 hours, the mushroom bacterium is reached contain a large amount of apolar substance generation Best Times 21, by the mode of supercritical extraction 4, can obtain and contain a large amount of apolar substance extracts 5, can extract the contained apolar substance of more mushroom bacterium.
Only the above only is one of the present invention preferred embodiment, when can not with qualification the present invention's scope.Promptly the equalization of being done according to the present patent application claim generally changes and modifies, and all should still belong in the scope that patent of the present invention contains.
Claims (7)
1. supercritical extraction technology for mushroom bacteria, its technology comprises;
Bacterial screening is selected the growth of fermenting of the mushroom bacterium of desire extraction;
The fermentation growth, to make its growth in the mushroom bacterium implantation culture medium, and under 20～35 ℃, cultivated about 72 hours～144 hours, the content that makes interior contained Biomass of glucose of mushroom bacterium and exopolysaccharide makes the apolar substance in the mushroom bacterium reach apolar substance generation Best Times for the highest;
The thalline drying with growing to the mushroom bacterium that apolar substance generates Best Times, is cultivated to take off on culture medium after about 72～144 hours and is carried out cold drying;
Supercritical extraction extracts action with the carbon dioxide abstraction agent of high pressure low temperature and through the dried mushroom bacterium of thalline;
Apolar substance extract, mushroom bacterium contain the liquid of a large amount of apolar substances via the extraction mode gained of supercritical extraction.
2. supercritical extraction technology for mushroom bacteria according to claim 1, bacterial screening wherein, the mushroom bacterium of selected desire extraction is the Antrodia camphorata sporophore that is suitable for after Antrodia camphorata thalline or the domestication.
3. supercritical extraction technology for mushroom bacteria according to claim 1, wherein fermentation growth can be liquid fermentation or solid fermentation, will mycelia or mushrooms implant in the liquid or solid culture medium and make its growth.
4. supercritical extraction technology for mushroom bacteria according to claim 1, wherein the exsiccant temperature of thalline is preferable with 25～35 ℃.
5. supercritical extraction technology for mushroom bacteria according to claim 1, the optimum condition of supercritical extraction wherein, to more than the 74atm, its temperature is lower than 31 ℃ and extracts action with the exert pressure of carbon dioxide abstraction liquid.
6. supercritical extraction technology for mushroom bacteria as claimed in claim 1 or 2, wherein the apolar substance of fermentation growth generates Best Times, on the Antrodia camphorata thalline, its Antrodia camphorata thalline was grown 72～144 hours under 30 ℃～35 ℃ temperature, and the content of the contained Biomass of glucose of Antrodia camphorata thalline and exopolysaccharide is higher at this moment.
7. supercritical extraction technology for mushroom bacteria according to claim 1, wherein the apolar substance of fermentation growth generates Best Times, also be useful on the Antrodia camphorata sporophore, about 180 days～480 days of its sporophore growth, and outward appearance is the gill fungus shape, the contained crude fat amount of Antrodia camphorata sporophore this moment is higher, and then can contain the extract of a large amount of crude fat by supercritical extraction technique.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910119321A CN101843642A (en) | 2009-03-24 | 2009-03-24 | Supercritical extraction technology for mushroom bacteria |
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| CN200910119321A CN101843642A (en) | 2009-03-24 | 2009-03-24 | Supercritical extraction technology for mushroom bacteria |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524752A (en) * | 2011-01-04 | 2012-07-04 | 北京电子科技职业学院 | Method for extracting volatile components from coprinus comatus |
| CN104970370A (en) * | 2015-08-13 | 2015-10-14 | 王跃进 | Method for preparing nutritional rice media microcapsule particles by using eighteen mushroom food materials |
| CN106176828A (en) * | 2015-03-06 | 2016-12-07 | 超微体生医科技股份有限公司 | Method for separating and purifying functional components in antrodia camphorata by supercritical fluid technology |
-
2009
- 2009-03-24 CN CN200910119321A patent/CN101843642A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524752A (en) * | 2011-01-04 | 2012-07-04 | 北京电子科技职业学院 | Method for extracting volatile components from coprinus comatus |
| CN106176828A (en) * | 2015-03-06 | 2016-12-07 | 超微体生医科技股份有限公司 | Method for separating and purifying functional components in antrodia camphorata by supercritical fluid technology |
| CN106176828B (en) * | 2015-03-06 | 2019-09-27 | 超微体生医科技股份有限公司 | Method for separating and purifying functional components in antrodia camphorata by supercritical fluid technology |
| CN104970370A (en) * | 2015-08-13 | 2015-10-14 | 王跃进 | Method for preparing nutritional rice media microcapsule particles by using eighteen mushroom food materials |
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Application publication date: 20100929 |