CN101842491B - Ethanol plant by-products is used for the purposes of yeast growth - Google Patents
Ethanol plant by-products is used for the purposes of yeast growth Download PDFInfo
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- CN101842491B CN101842491B CN200880114348.6A CN200880114348A CN101842491B CN 101842491 B CN101842491 B CN 101842491B CN 200880114348 A CN200880114348 A CN 200880114348A CN 101842491 B CN101842491 B CN 101842491B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
Embodiment of the present invention relate to, and such as, reduce the method for the heap(ed) capacity of organic acid and the glycerine be recycled in the water of zymotechnique.The organic acid produced during ethanol fermentation and glycerine are used as the surrogate of the carbohydrate of yeast growth.Yeast can be used as feed product to sell or is applied to follow-up fermentation.
Description
The cross reference of related application
This application claims the rights and interests of the U.S. Patent Application Serial Number 61/001,714 that on November 2nd, 2007 submits to.U.S. Patent Application Serial Number 61/001,714 are incorporated to by reference, as rewritten completely in herein.
The statement of federal funding research
Nothing.
Invention field
This instruction relates to but is not limited to following field: ethanol fermentation, yeast production and the refuse amount reduced from those techniques.
Background of invention
Hereafter comprise the information that can be used for understanding this instruction.This not admits, for current described or required theme, any one or material in information provided in this article are prior art, also not admit, by concrete or imply that any publication of quoting or document are prior art.
The background of prior art
Fig. 1 has set forth the typical process of substratum (media), inoculum, product and the byproduct stream introduced in the management of ethanol fermentation factory.In anaerobic fermentation tank 10, prepare ethanol by yeast (being generally the bacterial strain of yeast saccharomyces cerevisiae (Saccharomycescerevisiae)) anaerobically fermenting in the medium, described substratum comprises the dextrose (or other sugar or polysaccharide of C5 or larger) as primary carbon source.Before fermentation, yeast is bred by inoculating strain, and wherein inoculum is cultivated in the air blow tank of a succession of increase volume.Consisting of from the different water that may originate of those fermentor tanks, and corn mash and enzyme.
After ethanol fermentation completes, liquid stream is supplied in still kettle 30, in still kettle 30 by ethanol and described flow point from.Then, by from fermentation biomass and other particulate matter in separator 20 by gravity, centrifugal or filter and separation of fermentative broth.By comprise the drying of biomass, the solids-enriched that is separated, finally dry, and be commonly referred to distiller's dried grain (distillersdriedgrain, DDG).DDG sells usually used as animal-feed or animal feed components.
Residuum from the aqueous phase solution of fermention medium is commonly called " backflow (backset) ".Major carbonization compound in backflow is organic acid and the glycerine (" OAG ") of dilution.A part (usually about 35%) for backflow is reclaimed and turns back in initial anaerobic fermentation tank 10, with supplementary during fermentation must by the amount of water (or fresh water or process water) used.During anaerobically fermenting, organic acid and glycerine can not be used as carbon source by yeast; These compounds are formed by dextrose actually by yeast and contaminative organism (contaminatingorganism).Backflow is recycled in anaerobic fermentation tank 10 continuously and can causes suppressing gathering of the organic acid of alcohol production and glycerine.Therefore, in a continuous process, as shown in " other treatment step " in FIG, most backflow is directed in waste water or vapo(u)rization system.
In " other treatment step ", vapo(u)rization system is from a part for backflow recycle-water, and these water and initial backflow combine to be sent in anaerobic fermentation tank with other water source.Therefore, the residuum of aqueous phase is the concentrated solution of OAG, and this concentrated solution is passable, such as, by mixing with DDG also dry thus being used as animal-feed.OAG also may need to process as refuse.Usually, OAG is " negative value " product, and it can not increase the financial income (financialyield) of fermentation.
Invention summary
Embodiment relates to, and such as, reduces the method for the heap(ed) capacity (load) of organic acid (acetic acid and lactic acid) and the glycerine be recycled in the water of zymotechnique.Embodiment also relates to, and such as, ethanol fermentation by product is as the purposes of yeast production raw material.Additionally provide the method making yeast fermentation He prepare yeast-inoculated thing.
Organic acid and glycerine are the two kinds of by products formed during yeast anaerobically fermenting produces ethanol.I finds, and these by products of anaerobically fermenting are useful substrates of the yeast in aerobic fermentation.This has many beneficial effects.Such as, the carbon source carbon comprised in organic acid and glycerine being used as the aerobic cultivation of yeast is allowed.The yeast of this aerobic cultivation can be used as again the inoculum of anaerobically fermenting.
Embodiment of the present invention provide, such as, and the method for the OAG recirculation of producing during making the ethanol fermentation of yeast based on dextrose or other sugar.An embodiment provides the method being prepared ethanol by batch fermentation, and described method comprises: under the first culture condition (firstgrowthcondition), cultivate producing and ethanol microorganism in the first substratum, and described first substratum comprises carbon source.This carbon source comprise at least one organic acid (such as, acetic acid, lactic acid or both) and glycerine, they obtain with the by product by fermentation producing and ethanol in next life as culturing micro-organisms in the second substratum.Described second substratum comprises carbon source, such as has sugar or the carbohydrate of at least 5 carbon atoms; Microorganism is cultivated in the second substratum, produces ethanol and described by product.In a typical embodiment, producing and ethanol microorganism is yeast saccharomyces cerevisiae; And the first culture medium carbon source is substantially by water, micro-nutrients and form as the acetic acid of carbon source, lactic acid and glycerine, and described second substratum is made up of water, nutrient substance and the dextrose as carbon source substantially.In another embodiment, the first substratum comprises as the acetic acid of carbon source, lactic acid and glycerine, and the second substratum comprises the dextrose as carbon source.
Those of skill in the art will recognize that substratum of the present invention will comprise other nutrient substance required for microorganism culturing.The propagating culture medium of microorganism, except comprising at least one carbon source, comprises at least one nitrogenous source usually.Typical nitrogenous source is, such as, and ammonia, ammonium salt, amino acid, nitrate or nitrite.Outside denitrogenating, generally include other mineral substance.These comprise, and such as, potassium, magnesium, sodium, sulphur and phosphorus, it is included with the concentration between 0.05g/L to 2g/L usually.Other trace elements is included with the concentration level of mg/litre, such as, between 1mg/L and 5mg/L.Those elements can comprise, such as, and the iron of (ferric) of iron and/or (ferrous) form of ferrous iron, molybdenum, cobalt, calcium, zinc, manganese, iodine, copper and boron.Trace elements is present in substratum with their ionic species usually.
Other nutrient substance that can be present in substratum comprises VITAMIN, and described VITAMIN comprises vitamin B complexes VITAMIN.Nucleic acid precurser also can exist in the medium and utilize.As herein report in the productive culture base of the productive culture base of use, must component usually exist to be in excess in the form of requirement.It is also possible that may require in some applications to augment some in those components.
Further embodiment comprises the method by alcohol prepared by fermenting, described method comprises: in the first substratum, under aerobic conditions cultivate producing and ethanol microorganism to form inoculation meat soup, described first substratum comprises the carbon source containing organic acid and/or glycerine.To inoculate meat soup to mix with the second substratum, described second substratum comprises the carbon source containing the sugar and/or polysaccharide with at least 5 carbon atoms.Under anaerobic culturing micro-organisms is to produce ethanol and by product, and described by product comprises at least one composition of the group be made up of organic acid and glycerine.The source of described by product can also be the contaminative organism be usually present in ethanol fermentation.
In a typical embodiment, microorganism is yeast saccharomyces cerevisiae, and described first substratum forms by nutrient substance with as the lactic acid of carbon source, acetic acid, glycerine and water substantially; And described second substratum forms by nutrient substance with as the dextrose of carbon source and water substantially.In a further embodiment, the first substratum comprises as the lactic acid of carbon source, acetic acid and glycerine; And the second substratum comprises the dextrose as carbon source.In some embodiments, other nutrient substance needed for cultivation comprises in the liquid portion be recycled of OAG to be present in right amount.In some embodiments, supplement nitrogen may be needed to cultivate, and in a further embodiment, in the current be recycled, there is not appropriate nitrogen and other nutrient substance, also may need to augment some nutrient substances.
One also further embodiment comprise a kind of by the method for fermentation for principal product (primaryproduct), described method comprises: under the first fermentation condition in the first substratum, with cultured continuously mode culturing micro-organisms, produce the required inoculum that ferments under a second condition, described first substratum comprises the by product that same microorganism culture ferments under a second condition, and described second condition is selected as producing described principal product; Under the second fermentation condition in the second substratum culturing micro-organisms culture to produce principal product and to comprise the by product in the macronutrient source from step 1; The by product comprising macronutrient source is separated to obtain byproduct stream with the second substratum with principal product; And provide byproduct stream to come with the aerobic culturing micro-organisms of cultured continuously mode.
In a further embodiment, method described herein comprises: the further step concentrating byproduct stream in the first substratum before culturing micro-organisms.The byproduct stream of larger concentration creates the higher biomass obtained.The fermentation condition of embodiment of the present invention can be aerobic.If OAG is used as substrate, fermenting to be aerobic.
The culture cultivated in the first substratum can be used as batch culture and breeds as continuous culture.When cultivating culture in a continuous manner, this providing utilization and being far smaller than the ability of presently used production for the fermenter volume of the inoculum of subordinate phase.This provide the more good utilisation to fermentor tank and subsystem.Compared to current reached in industry, application is continuously fermented can also the fermentor tank of applied culture inoculum more.Utilization ratio is up to 16 times of utilization ratio of previous processes.
The current method of inoculum breeding depends on two kinds of techniques: enzyme catalysis starch-splitting or other carbon source can the sugar that utilize of cultured object to produce, and the cultivation of culture depends on the speed of starch degradation.Method provided in this article has the substrate being easy to obtain being easy to the amount quantized.The reproducibility of inoculum and reproducibility and the consistence of consistence tendency for being better than art methods.
Applying in some or all experiments carried out of instructing herein, for the OAG of each consumed 1g/L, obtain at least about 1 × 10
8the yeast productive rate of yeast cell/ml substratum.Obtain 1.5 × 10
9the yeast concn of cell/ml and 3 × 10
8cell/L/hr to 4 × 10
8the growth velocity (growthrate) of the cultured continuously of cell/L/hr.
Usually, use yeast strain to ferment, described yeast strain is generally the bacterial strain of yeast belong, and is more typically the bacterial strain of yeast saccharomyces cerevisiae.Microorganism can also be the bacterial strain of the bacterial strain of such as coryneform bacteria (Corynebacterium), the bacterial strain of staphylococcus (Staphylococcus) or listeria bacteria (Listeria).
Embodiment 1
The batch fermentation tank that embodiment 1 reports the yeast saccharomyces cerevisiae utilizing organic acid and glycerine is cultivated, and described organic acid and glycerine are by product as the evaporation of the backflow from dry grind ethanol production unit and obtain.Yeast growth has in batches been carried out in two 3.5L fermentor tanks.Between 5 to 10 times of OAG concentration initial for OAG simmer down to.
Sodium hydroxide is used to be 4.4 by the pH regulator of culture.Also with the form of ammonium salt, nitrogen is joined in mixture, until the concentration of nitrogen (in ammonia) is 2.5g/L.Inoculation ratio is 1%v/v.
Result is shown in Figure 4.Organic acid is the preferred meta-bolites of yeast.To glycerine is preferably secondary.Organic acid is exhausted and is indicated by the remarkable increase of dissolved oxygen concentration, and described dissolved oxygen concentration is not shown in figure.
Culture reached about 9 × 10 in 24 hours
8cell/ml and use the carbon source of 15-17g/L at this time durations.Lactic acid and acetic acid are eliminated to detection threshold, and the glycerine of about 40% has mainly been utilized when organic acid is depleted and between the end of run.Initial culture is cultivated by ammonia being used as nitrogenous source and being used for pH regulator to strengthen simultaneously.
Embodiment 2
Embodiment 2 reports the cultivation of the yeast saccharomyces cerevisiae utilizing organic acid and glycerine, and described organic acid and glycerine are by product as the evaporation of the backflow from dry grind ethanol production unit and obtain.Fermentation initially carrying out as batch process, then reach be considered to abundance more than 5 × 10
8yeast amount (yeastmass) after be moved to continuous fermentation tank.
After being moved to pattern of continuously fermenting, fermenting reaches steady state conditions rapidly with the retention time of 5 to 6 hours.As shown in Figure 5, as passed through optical density(OD) (OD at 660nm) or maintaining stable in the time length of continuous print charging/drippage phase (dropphase) by the culture density that living yeast counting represents, this maintains 90 hours.Create about 16 fermenter volume in the meantime.Average yeast concn during external phase is 1.2 × 10
9cell/ml.
Fig. 6 shows the consistence of the fermentation described in the present embodiment.Less change in retention time is mainly the result of the difference assessment of the fermenter volume run.As embodiment 1, yeast growth in this embodiment requires the nitrogenous source of carbon source and interpolation.Also wish to be best cultivation level (about 4.0 to 5.5) by pH regulator.
Embodiment 3
Embodiment 3 shows and utilizes organic acid yeast culture according to another embodiment of the invention.Condition is identical with embodiment 2, but organic acid is not concentrated.The present embodiment the results are shown in Fig. 7.Cultivate and be subject to the restriction of low concentration of substrate, as when result compared to embodiment 2 indicated by low optical density(OD) and yeast concn.
That quote herein or mentioned patent, patent application, publication, technical paper, books and other document and material, from the date that each publication is written, illustrate the state of the art of the technical field of the invention technician, and all by reference to being incorporated to, as rewritten completely in herein.Comprising document is in this manual not admit that described document represents previous invention or for prior art because of any object.
The term adopted herein and expression be non-limiting term as exemplary term, and do not attempt this term and expression or its any part to be used for get rid of any coordinator that is known or that developed afterwards, no matter whether such coordinator is set forth in this article or is shown or is described, and also no matter whether such coordinator is regarded as predictable; But will be appreciated that, various amendments falls into the scope of the present invention for required protection, no matter change or correction are carried out or do not carried out to those claims issued whether with any reason.Therefore; should be appreciated that; although specifically disclose the present invention by preferred embodiment and optional feature; but those skilled in the art can adopt wherein specialize or modifications and variations of the present invention disclosed herein, and such modifications and variations are considered to fall into disclosed herein and scope that is the present invention for required protection.
Concrete grammar described herein and composition represent preferred embodiment, and are exemplary and do not expect for limiting the scope of the invention.Those skilled in the art can understand after this specification sheets of consideration other object, in and embodiment, and these other object, aspect and embodiments are comprised in the spirit of the present invention of the scope definition of claim.In the presented embodiments, described explanation should be interpreted as including but are not limited to those embodiments.
Those skilled in the art can easily obviously find out, the substitutions and modifications that can change invention disclosed herein, and do not depart from the scope of the present invention and spirit, and do not depart from explanation of the present invention, described explanation comprises those explanations of illustratively setting forth herein, clearly, the thought that various modifications and coordinator can be used in the present invention and do not depart from its scope.It will be recognized by those of ordinary skills, can in form and on specifically, make change and do not depart from the spirit and scope of the present invention.Described embodiment should be considered illustrative and nonrestrictive in all respects.Therefore, such as, other embodiment falls into scope of the present invention and falls into claim below.
Accompanying drawing is sketched
Fig. 1. Fig. 1 describes the typical process at ethanol fermentation factory management substratum and byproduct stream.
Fig. 2. Fig. 2 describes an embodiment of the improvement provided by this instruction.
Fig. 3. comparing of the improving technique that the typical process that Fig. 3 describes Fig. 1 provides with the disclosure.Exemplary process steps is shown by dotted line.
Fig. 4. Fig. 4 display as in embodiment 1 report, according to the result of the aerobic batch yeast growth of an embodiment.
Fig. 5. Fig. 5 shows the viable count (measure with cell number and pass through photo densitometry) continuously fermented reported in example 2.
Fig. 6. Fig. 6 shows the charging interpolation of continuously fermenting in embodiment 2 and retention time.
Fig. 7. Fig. 7 display as in embodiment 3 the viable count of the yeast culture utilizing unconcentrated OAG to carry out reported.
Detailed Description Of The Invention
This instruction describes some different characteristic sum aspects of the present invention according to various exemplary.But should be appreciated that and present invention encompasses many selectable embodiments, it is by thinking that with those of ordinary skill in the art any one combination in different characteristic sum aspect described herein of the mode of useful any combination has been come.
Treatment process as described herein and product can provide the many advantages exceeding prior art.Certainly, scope of the present invention is defined by the claims, and whether embodiment falls into this scope should not be limited to described method and whether provide the one or more of these advantages.
The explanation of at least one embodiment of the present invention is by carrying out with reference to accompanying drawing.Fig. 2 describes an embodiment of the improvement provided by this instruction.Not by the major part guiding waste water of the condensed organic be recycled and the rudimentary animal-feed (such as DDG) that leads, but by concentrated OAG (" cOAG ") for providing main carbon source to the second fermentation in aerobic fermentation tank 5A.When the dextrose not having to add or other sugar, organic acid and glycerine are consumed by yeast during aerobic fermentation.
The concentration joining the OAG of the first aerobic fermentation can change with their source; Such as, they can change with original equipment (originatingfacility).Nitrogenous source is present in slight amount in the stream be recycled usually, but if carbon-nitrogen ratio may must be added in the first fermentation higher position very much.Find that other nutrient substance is present in tested recirculation flow with appropriate amount, but use the stream from other source may require to add one or more nutrient substances.
Water content changes usually, if expect to have highly filled recirculation flow, is then less than 80%; If or the recirculation flow that expectation has compared with low solid content, be then between 80% and 99%.The solid tolerance of system can be depending on many factors, and described factor comprises: the source of recirculation flow and in fermentor tank on the impact of osmotic pressure.
OAG fermentation is for aerobic.The utilization ratio of OAG can be limited to the oxygen transfer rate in OAG fermentation.This can be useful, such as, if expect slower fermentation, such as, to maintain the heat release from fermentation of lower level.
The biomass containing yeast obtained in aerobic fermentation tank 5A can be used as inoculum, come in anaerobic fermentation tank 10, cause anaerobically fermenting further and generate ethanol.It such as directly can also be used as the animal-feed of better quality with the form of DDG.
Do not require the cOAG of certain concentration.Usually, both are proportional, and lower cOAG concentration can cause lower yeast output.Organic acid in backflow and glycerine and be provided in table 1 as the organic acid of enriched material and typical case's composition of glycerine.Certainly, those numerical value can change with fermentation and concentrated character.
Table 1
| Quantity of reflux | Enriched material amount | |
| Acetic acid | 400-2500mg/L | 1500-7000mg/L |
| Lactic acid | 800-4000mg/L | 4000-15,000mg/L |
| Glycerine | 5-20g/L | 20-50g/L |
In typical practice continuously, correct the amount of the cOAG be fed in aerobic fermentation tank 5A, make the glycerine of all organic acid and about 25-75% substantially be consumed by yeast and be converted into biomass.With regard to " substantially whole ", its represents that the organic acid being greater than 95% be fed in fermentor tank is consumed in batch fermentation.Because first aerobic fermentation consume organic acid, so correct fermentation to be less than for consuming the amount that substantially whole organic acids can reduce the glycerine be consumed widely.In a preferred embodiment, substantially whole organic acids and the glycerine of about 15% to 45% are consumed.
A kind of method of the wear rate of adjustable OAG is the pH by regulating fermentation.In one typically fermentation, by adding ammonia or ammonium salt, pH is maintained at 4.2 to 4.8.In the fermentation of one embodiment of the invention, by adding alkali, pH being maintained between 5.0 and 6.5, being generally about 5.5.This alkali can be, such as, and ammonia or sodium hydroxide.Usual use ammonia, this is because it can also provide nitrogenous source for the cultivation of yeast.
Along with organic acid is consumed, the pH of the meat soup in fermentor tank can rise.Higher pH level is the result of this phenomenon.If needed, the ammonium salt as nitrogenous source can alleviate this problem.Along with nitrogen is utilized, pH can be lowered.
Although other additive needed for biomass breeding can be contained in anaerobically fermenting, when the anaerobically fermenting producing OAG stream, when dry grinding (dry-grind) ethanol production process a part of, does not usually need additive.When the product that OAG stream is wet-milling fermentation, adding of some micro-nutrientss can be useful or or even necessity.
The speed extracting inoculum or DDG from aerobic fermentation tank 5A can realize balancing with the feeding rate of cOAG and the wear rate forming biomass, make in fact, fermention medium in aerobic fermentation tank 5A contains the glycerine of steady-state level and very low-level organic acid, simultaneously continuous seepage biomass.If add the second reactor in a row, then can obtain low-level glycerine.In such reactor, glycerine will preferably be used as independent carbon source.
Although yeast is used as the Exemplary organisms that can utilize aerobic fermentation and anaerobically fermenting by the embodiment presented and explanation, five carbon and six carbon substrates can be used can to benefit from the present invention as other facultative organism of nutriment (fuel) herein.Embodiment of the present invention, based on the purposes of different nutriment for different types of fermentation, provide the method for carbonaceous by-products as the substrate of aerobic fermentation of use anaerobically fermenting.Except yeast, the organism of these methods can be utilized to comprise, such as facultative anaerobe, the member in such as proteus (Proteus), serratia (Serratia), Ou Wenshi vibrios (ErwiniaVibrio), Aeromonas (Aeromonas) and Photobacterium (Photobacterium).
This instruction provides the some advantages exceeding prior art processes.Certainly, these advantages should not be interpreted as prerequisite or restriction, unless they are contained in claim clearly.The first, its reduction must be treated to the OAG of aqueous waste and the amount of other component.Second, it is by carrying out further alcohol production with the form of the inoculum based on non-carbohydrate by other negative value organic acid and the glycerin by-products DDG being converted into high value or more the yeast of ethanol fermentation, and catches (capture) from other negative value organic acid and glycerin by-products and be worth.Finally, which reduce and too many OAG backflow is incorporated in the restraining effect of association in the second initial fermentation, catch simultaneously and re-use some of the water in system.In figure 3, embodiment of the present invention and prior art processes are compared.Usually, the part of guiding the water be recycled of inoculating strain into is less than the part of guiding the second fermentation into.
Claims (15)
1., by a method for alcohol prepared by fermenting, described method comprises:
A () cultivates producing and ethanol microorganism to form inoculation meat soup under the first culture condition in the first substratum, described first substratum comprises the carbon source containing at least one composition being selected from the group be made up of organic acid and glycerine;
B () inoculates the second substratum with the inoculation meat soup obtained from step (a), and in described second substratum, described microorganism is cultivated under the second condition of applicable production ethanol, described second substratum comprises the carbon source of at least one composition containing the group be made up of the sugar with at least 5 carbon atoms;
(c) distillation ethanol, and remove described microorganism to obtain backflow from described second substratum; And
D () uses the described backflow obtained from step (c) as the described first substratum repeating step (a) to (c) step (a).
2. method according to claim 1, wherein said sugar is polysaccharide.
3. method according to claim 1, wherein said producing and ethanol microorganism is yeast saccharomyces cerevisiae (Saccharomycescerevisiae), described first substratum is made up of water, acetic acid, lactic acid and glycerine substantially, and described second substratum is made up of water and dextrose substantially.
4., by a method for alcohol prepared by fermenting, described method comprises:
A () under aerobic conditions cultivates producing and ethanol microorganism to form inoculation meat soup in the first substratum, described first substratum comprises the carbon source containing at least one composition being selected from the group be made up of organic acid and glycerine;
B described inoculation meat soup mixes with the second substratum by (), described second substratum comprises the carbon source of at least one composition containing the group be made up of the sugar with at least 5 carbon atoms;
C () under anaerobic cultivates described microorganism to produce ethanol and by product, described by product comprises at least one composition of the group be made up of organic acid and glycerine;
D () distills described second substratum to remove ethanol, to form the backflow substratum comprising described by product; And
E () uses the described backflow substratum that obtains from step (d) as the described first substratum repeating step (a) to (d) of step (a).
5. method according to claim 4, described sugar is polysaccharide.
6. method according to claim 4, wherein said microorganism is yeast saccharomyces cerevisiae, and described first substratum is made up of lactic acid, acetic acid, G & W substantially, and described second substratum is made up of water and dextrose substantially.
7., by a method for alcohol prepared by fermenting, described method comprises:
A () is cultivated the yeast culture producing described ethanol and is inoculated meat soup to be formed under the first fermentation condition in the first substratum, the by product of described first substratum identical yeast culture fermentation under being included in the fermentation condition being selected as producing described ethanol, described by product comprises the first nutrition sources;
B () inoculates the second fermention medium with the described inoculation meat soup obtained from step (a), and in described second substratum, described yeast culture is cultivated under described second fermentation condition, wherein said second substratum comprises dextrose, and described second fermentation condition is selected to produce ethanol and comprised the by product of the first nutrition sources;
C the described by product of described first nutrition sources with described yeast that comprise step (b) is separated to obtain with described second substratum the byproduct stream comprising described first nutrition sources with described ethanol by (); And
D (), by providing first substratum of described byproduct stream as step (a) of step (c), carrys out repeating step (a) to (c) to cultivate described yeast culture according to step (a) thus to form described inoculation meat soup.
8. method according to claim 7, before described method comprises the steps: to cultivate described yeast culture in described first substratum, concentrated described byproduct stream.
9. method according to claim 7, wherein said first fermentation condition is aerobic, and described second fermentation condition is anaerobism.
10. method according to claim 7, wherein said first substratum is made up of lactic acid, acetic acid, water and glycerine substantially.
11. methods according to claim 7, wherein said second substratum is made up of water and dextrose substantially.
12. methods according to claim 8, wherein said first fermentation condition is aerobic, described second fermentation condition is anaerobism, described first substratum is made up of lactic acid, acetic acid, water and glycerine substantially, described second substratum is made up of water and dextrose substantially, and described yeast culture is Cultures of S. cerevisiae.
13. methods according to claim 7, wherein said yeast culture is Cultures of S. cerevisiae.
14. methods according to claim 3, described method comprises: the culturing step carrying out step (a) in continuous fermentation tank, and wherein said yeast concn is at least 1.5 × 10
9cell/ml, and growth velocity is at least 3 × 10
8cell/L/hr.
15. methods according to claim 1, wherein said fermentation is selected from by the group of continuously fermenting and batch fermentation forms.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US171407P | 2007-11-02 | 2007-11-02 | |
| US61/001,714 | 2007-11-02 | ||
| PCT/US2008/081918 WO2009059084A1 (en) | 2007-11-02 | 2008-10-31 | Use of ethanol plant by-products for yeast propagation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101842491A CN101842491A (en) | 2010-09-22 |
| CN101842491B true CN101842491B (en) | 2015-11-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200880114348.6A Active CN101842491B (en) | 2007-11-02 | 2008-10-31 | Ethanol plant by-products is used for the purposes of yeast growth |
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| Country | Link |
|---|---|
| US (1) | US8183022B2 (en) |
| CN (1) | CN101842491B (en) |
| BR (1) | BRPI0817137B1 (en) |
| WO (1) | WO2009059084A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AR074261A1 (en) * | 2008-11-04 | 2011-01-05 | Highmark Renewables Res Ltd Partnership | INCREASED FERMENTATION OF ETHANOL USING BIODIGESTATE |
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| KR101011244B1 (en) * | 2010-08-23 | 2011-02-07 | 송백영 | Method for preparing bioethanol from watermelon seeds |
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| CN1006902B (en) * | 1984-12-07 | 1990-02-21 | 艾尔费拉瓦尔公司 | Process for the production of ethanol |
| CN1069768A (en) * | 1991-05-17 | 1993-03-10 | 索尔维酶有限公司 | Preparation alcoholic acid method |
| US20070184541A1 (en) * | 2004-06-25 | 2007-08-09 | Karl Daniel W | Corn fractionation method |
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- 2008-10-31 CN CN200880114348.6A patent/CN101842491B/en active Active
- 2008-10-31 US US12/262,315 patent/US8183022B2/en active Active
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1006902B (en) * | 1984-12-07 | 1990-02-21 | 艾尔费拉瓦尔公司 | Process for the production of ethanol |
| CN1069768A (en) * | 1991-05-17 | 1993-03-10 | 索尔维酶有限公司 | Preparation alcoholic acid method |
| US20070184541A1 (en) * | 2004-06-25 | 2007-08-09 | Karl Daniel W | Corn fractionation method |
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| WO2009059084A1 (en) | 2009-05-07 |
| CN101842491A (en) | 2010-09-22 |
| BRPI0817137B1 (en) | 2020-12-01 |
| BRPI0817137A2 (en) | 2020-03-10 |
| US8183022B2 (en) | 2012-05-22 |
| US20090117632A1 (en) | 2009-05-07 |
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