CN101838647A - Promoter BgIosP587, and preparation method and application thereof - Google Patents
Promoter BgIosP587, and preparation method and application thereof Download PDFInfo
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- CN101838647A CN101838647A CN200910238992A CN200910238992A CN101838647A CN 101838647 A CN101838647 A CN 101838647A CN 200910238992 A CN200910238992 A CN 200910238992A CN 200910238992 A CN200910238992 A CN 200910238992A CN 101838647 A CN101838647 A CN 101838647A
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- anhui rice
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Abstract
Description
Claims (10)
- One kind have nucleotide sequence shown in the SEQ ID NO:1 or with the promotor of its complementary nucleotide sequence, or it has the following variant of being selected from of promoter function:1) under high stringent condition with the nucleotide sequence of the nucleotide sequence hybridization shown in the SEQ ID NO:1,2) to the nucleotide sequence shown in the SEQ ID NO:1 carry out one or more bases replacement, disappearance, interpolation modified nucleotide sequences and3) has the nucleotide sequence of at least 90% sequence identity with the nucleotide sequence shown in the SEQ ID NO:1.
- 2. a recombinant vectors is characterized in that, described recombinant vectors contains the described promotor of claim 1, and preferably, described recombinant vectors is the p8+P587 recombinant vectors.
- 3. reconstitution cell that contains the described recombinant vectors of claim 2.
- 4. the described reconstitution cell of claim 3, it is reorganization Agrobacterium EHA105-P587.
- 5. monocotyledons callus, it is characterized in that, described callus transforms the requirement 1 described promotor of having the right, preferably, described callus is the rice callus tissue, more preferably, described paddy rice is that Japan is fine, in spend 9, in spend 10, in spend 11, the Taibei 309, No. 8, Danjiang River, cloud rice No. 2, Shanyou 63, Shan excellent 608, Feng You 22, Guizhou Province excellent 88, II excellent 416, II excellent 107, II excellent 128, II excellent 718, accurate two excellent 527, No. 1, river farming, assorted 0152, Anhui rice 88, Anhui rice 90, Anhui rice 92, Anhui rice 94, Anhui rice 96, Anhui rice 185, Anhui rice 187, Anhui rice 189, Anhui rice 191, Anhui rice 193, Anhui rice 195, Anhui rice 197, Anhui rice 199, Anhui rice 201, Anhui rice 203, Anhui rice 205, Anhui rice 207, and Tianjin former 101, particularly preferably, described paddy rice is that Japan is fine.
- 6. a method for preparing the promotor described in the claim 1 comprises the steps:1) according to the nucleotide sequence shown in the SEQ ID NO:1, design pcr amplification primer is right, and particularly, described pcr amplification primer is to being SEQ ID NO:2 and SEQ ID NO:3;2) be template with the fine genomic dna of paddy rice Japan, use pcr amplification primer designed in the step 1) carrying out pcr amplification.
- 7. method of regulating and control genetic expression in the monocotyledons, described method comprises that preferably, described callus is the rice callus tissue with the step of the callus of the described promotor transforming monocots of claim 1, particularly preferably, described paddy rice is that Japan is fine.
- 8. method according to claim 7 has been utilized the described reorganization of claim 4 Agrobacterium EHA105-P587 in the conversion process of wherein said monocotyledons callus.
- 9. the described promotor of claim 1 purposes of destination gene expression in the regulation and control monocotyledons, wherein said goal gene is GUS, and described monocotyledons is a paddy rice, and preferably, described paddy rice be Japanese fine.
- 10. the purposes of the described promotor of claim 1 in rice breeding, preferably, described paddy rice is that Japan is fine, in spend 9, in spend 10, in spend 11, the Taibei 309, No. 8, Danjiang River, cloud rice No. 2, Shanyou 63, Shan excellent 608, Feng You 22, Guizhou Province excellent 88, II excellent 416, II excellent 107, II excellent 128, II excellent 718, accurate two excellent 527, No. 1, river farming, assorted 0152, Anhui rice 88, Anhui rice 90, Anhui rice 92, Anhui rice 94, Anhui rice 96, Anhui rice 185, Anhui rice 187, Anhui rice 189, Anhui rice 191, Anhui rice 193, Anhui rice 195, Anhui rice 197, Anhui rice 199, Anhui rice 201, Anhui rice 203, Anhui rice 205, Anhui rice 207, and Tianjin former 101, particularly preferably, described paddy rice is that Japan is fine.
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