CN101832982A - Method and device for testing oxygen permeation of cornea repair material - Google Patents
Method and device for testing oxygen permeation of cornea repair material Download PDFInfo
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- CN101832982A CN101832982A CN201010161752A CN201010161752A CN101832982A CN 101832982 A CN101832982 A CN 101832982A CN 201010161752 A CN201010161752 A CN 201010161752A CN 201010161752 A CN201010161752 A CN 201010161752A CN 101832982 A CN101832982 A CN 101832982A
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Abstract
The invention relates to a method and a device for testing the oxygen permeation of a cornea repair material. A sample is placed in a sample clamp; oxygen and carrier gas are introduced to two sides of the sample; and the oxygen passes through the sample and is taken to a detection device by the carrier gas for detecting the concentration of the oxygen. The test device comprises the sample clamp which consists of an oxygen cavity and a carrier gas cavity; an inlet end of the oxygen cavity is communicated with the oxygen through an inlet pipeline of the oxygen cavity; an outlet end of the oxygen cavity is evacuated through an outlet pipeline of the oxygen cavity; and an inlet end of the carrier gas cavity is communicated with the carrier gas through an inlet pipeline of the carrier gas cavity, and an outlet end of the carrier gas cavity is communicated with the detection device through an outlet pipeline of the carrier gas cavity. Compared with a coulomb sensor, a gas chromatograph used by the invention has lower price, lower repair and maintenance frequency and longer service cycle, and the operation cost is obviously reduced.
Description
Technical field
The present invention relates to a kind of oxygen permeation of cornea repair material method of testing and device, especially can realize the oxygen permeating amount of same device to test contact lens,soft, contact lens,hard, artificial cornea and other cornea repair materials.
Background technology
Oxygen permeability is the important indicator of cornea repair material performance, and it is the important step that cornea repair material characterizes that oxygen permeability detects.
Corresponding to dissimilar cornea repair materials, the detection of oxygen permeating amount is also different: generally use polarography for contact lens,soft and artificial cornea, sample is invested on the electrode, oxygen is by sample and after separating, on the two poles of the earth of electrode electrochemical reaction taking place, can obtain oxygen permeating amount by detecting the electric current that produces and calculating; Generally use a coulomb method for contact lens,hard, contact lense is placed sample clamp, a side passes to oxygen, and opposite side passes to carrier gas, and the oxygen infiltration utilizes enclosed pasture sensor concentration of oxygen by being taken away by carrier gas behind the contact lense.
Polarography can only be measured the oxygen permeability coefficient of contact lens,soft and artificial cornea, uses extremely limited.The enclosed pasture method generally is used for the mensuration of the oxygen permeability coefficient of contact lens,hard, also can be used for the mensuration of the oxygen permeability coefficient of contact lens,soft, but the enclosed pasture sensor price height in the enclosed pasture method, and it is the consumption-type equipment, the maintenance maintenance is frequent, and life cycle is short, and running cost is higher.
Summary of the invention
Purpose of the present invention is exactly in order to solve the single problem of present oxygen permeation of cornea repair material proving installation tested object, provide a kind of simple in structure, can realize the oxygen permeation of cornea repair material method of testing and the device thereof of the oxygen permeating amount of same device to test contact lens,soft, contact lens,hard, artificial cornea and other cornea repair materials.
For realizing purpose of the present invention, the present invention adopts following technical scheme:
A kind of method of testing of oxygen permeation of cornea repair material may further comprise the steps:
(1) in gas chromatograph, advances volume V
1Oxygen, from spectrogram, obtain peak area S
1
(2) logical respectively oxygen in the both sides of testing sample and carrier gas, oxygen is penetrated into carrier gas one side through the zone that sample is exposed to Oxygen Flow, with volume V
2Oxygen and the mixed gas of carrier gas send into gas chromatograph, detect the amount of oxygen in the mixed gas, from spectrogram, obtain peak area S
2
(3) the oxygen permeability coefficient Dk of sample, oxygen permeating amount Dk/t can calculate by following formula:
Dk＝(V
1×S
2×t×Q)/(S
1×A×p×V
2)
Dk/t＝(V
1×S
2×Q)/(S
1×A×p×V
2)
Wherein, Dk is an oxygen permeability coefficient, and unit is mlcmcm
-2S
-1Pa
-1Dk/t is an oxygen permeating amount, and unit is mlcmcm
-3S
-1Pa
-1V
1Be the volume of purity oxygen standard specimen, unit is ml; S
1It is the gas chromatogram peak area of purity oxygen standard specimen; T is the harmonic average thickness that sample is exposed to the zone in the Oxygen Flow, and unit is cm; Q is the flow of carrier gas, and unit is mls
-1A is the area that sample is exposed to the zone of Oxygen Flow, and unit is cm
2P is that sample both sides oxygen pressure is poor, and unit is Pa, and its value equals atmospheric pressure; V
2Be the volume of the mixed gas of carrier gas and oxygen, unit is ml; S
2It is the peak area of sending into the oxygen in the mixed gas of gas chromatograph.
Described carrier gas comprises nitrogen, hydrogen or helium.
The device of realizing a kind of method of testing of oxygen permeation of cornea repair material mainly comprises sample clamp 20, oxygen admission gear, carrier gas admission gear and pick-up unit, and described sample clamp 20 is made up of oxygen chamber 21 and carrier gas cavity 24; Oxygen chamber 21 is connected with the oxygen admission gear by oxygen chamber admission line 7, and the oxygen chamber also is provided with oxygen chamber outlet pipe 8; Carrier gas cavity 24 is connected with the carrier gas admission gear by carrier gas cavity admission line 15, and links to each other with pick-up unit by carrier gas cavity outlet pipe 16; Described pick-up unit comprises quantitative sampling device 17 and gas chromatograph 19.
Described oxygen admission gear comprises oxygen flow meter 6, oxygen channel III5, oxygen flow variable valve 4, oxygen channel I 1 and the welding bottle that connects successively.
Described carrier gas admission gear comprises carrier gas flux meter 14, carrier gas channel III13, carrier gas flux variable valve 12, carrier gas channel I 9 and the carrier gas bottle that connects successively.
Described oxygen chamber 21 is connected with screw with the chamber wall joint of carrier gas cavity 24.
The sample that is positioned at the chamber wall joint of described oxygen chamber 21 and carrier gas cavity 24 is provided with O-ring seal 23 or applies vacuum silica gel.
Outlet side, described oxygen chamber communicates with the external world by oxygen chamber outlet pipe 8.
Described quantitative sampling device 17 can adopt the quantitative sampling device of prior art, and as six-way valve and quantitative ring that employing is connected, six-way valve is connected with the injection port of gas chromatograph.
Described oxygen chamber admission line 7, oxygen chamber outlet pipe 8, carrier gas cavity outlet pipe 16, pick-up unit pipeline 18, oxygen channel III5, oxygen channel I 1, carrier gas channel III13 and carrier gas channel I 9 are tracheae.
The present invention with respect to advantage and the beneficial effect that prior art has is:
(1), same device can be realized the oxygen permeating amount test of contact lens,soft, contact lens,hard, artificial cornea and other cornea repair materials.
(2), the gas chromatograph price is far below the enclosed pasture sensor, and maintenance maintenance frequency the enclosed pasture sensor is low, life cycle has significantly reduced running cost far above the enclosed pasture sensor.
Description of drawings
Fig. 1 is the structural drawing of measurement mechanism of the present invention;
Fig. 2 is the structural drawing of the sample clamp of mensuration contact lense;
Fig. 3 is the structural drawing of the sample clamp of other cornea repair materials of mensuration except that contact lense.
Wherein, 1. oxygen channel I, 4. oxygen flow variable valve, 5. oxygen channel III, 6. oxygen flow meter, 7. oxygen chamber admission line, 8. oxygen chamber outlet pipe, 9. carrier gas channel I, 12. the carrier gas flux variable valve, 13. carrier gas channel III, 14. carrier gas flux meters, 15. carrier gas cavity admission lines, 16. the carrier gas cavity outlet pipe, 17. quantitative sampling devices, 18. pick-up unit pipelines, 19. gas chromatograph, 20. sample clamps, 21. oxygen chambeies, 22. sample, 23. O-ring seals, 24. carrier gas cavity.
Embodiment
The invention will be further described below in conjunction with accompanying drawing and embodiment.
Method of testing implementation step of the present invention is:
(1) in gas chromatograph, advances volume V
1Oxygen, from spectrogram, obtain peak area S
1
(2) logical respectively oxygen in the both sides of testing sample and carrier gas, oxygen is penetrated into carrier gas one side through the zone that sample is exposed to Oxygen Flow, with volume V
2Oxygen and the mixed gas of carrier gas send into gas chromatograph, detect the amount of oxygen in the mixed gas, from spectrogram, obtain peak area S
2
(3) the oxygen permeability coefficient Dk of sample, oxygen permeating amount Dk/t can calculate by following formula:
Dk＝(V
1×S
2×t×Q)/(S
1×A×p×V
2)
Dk/t＝(V
1×S
2×Q)/(S
1×A×p×V
2)
Wherein, Dk is an oxygen permeability coefficient, and unit is mlcmcm
-2S
-1Pa
-1Dk/t is an oxygen permeating amount, and unit is mlcmcm
-3S
-1Pa
-1V
1Be the volume of purity oxygen standard specimen, unit is ml; S
1It is the gas chromatogram peak area of purity oxygen standard specimen; T is the harmonic average thickness that sample is exposed to the zone in the Oxygen Flow, and unit is cm; Q is the flow of carrier gas, and unit is mls
-1A is the area that sample is exposed to the zone of Oxygen Flow, and unit is cm
2P is that sample both sides oxygen pressure is poor, and unit is Pa, and its value equals atmospheric pressure; V
2Be the volume of the mixed gas of carrier gas and oxygen, unit is ml; S
2It is the peak area of sending into the oxygen in the mixed gas of gas chromatograph.
Carrier gas is a nitrogen.
Embodiment 2
As illustrated in fig. 1 and 2, oxygen chamber 21 is the cambered surface that matches with the chamber wall joint of carrier gas cavity 24, with the solid shape is the sample of cambered surface, contact lense oxygen permeating amount proving installation mainly comprises sample clamp 20, oxygen admission gear, carrier gas admission gear and pick-up unit among the present invention, and described sample clamp 20 is made up of oxygen chamber 21 and carrier gas cavity 24; Oxygen chamber 21 is connected with the oxygen admission gear by oxygen chamber admission line 7, and the oxygen chamber also is provided with oxygen chamber outlet pipe 8; Carrier gas cavity 24 is connected with the carrier gas admission gear by carrier gas cavity admission line 15, and links to each other with pick-up unit by carrier gas cavity outlet pipe 16; Described pick-up unit comprises quantitative sampling device 17 and gas chromatograph 19.
The oxygen admission gear comprises oxygen flow meter 6, oxygen channel III5, oxygen flow variable valve 4, oxygen channel I 1 and the welding bottle that connects successively.
The carrier gas admission gear comprises carrier gas flux meter 14, carrier gas channel III13, carrier gas flux variable valve 12, carrier gas channel I 9 and the carrier gas bottle that connects successively.
The sample that is positioned at the chamber wall joint of oxygen chamber 21 and carrier gas cavity 24 is provided with O-ring seal 23.
Outlet side, oxygen chamber communicates with the external world by oxygen chamber outlet pipe 8.
Six-way valve is when dress sample position, and the combination gas that carrier gas cavity outlet pipe 16 flows out enters quantitative ring by six-way valve, and after quantitatively ring was full of, unnecessary combination gas was discharged from the relieving device of six-way valve; When six-way valve turned to the sample introduction position, quantitative ring disconnection was connected with carrier gas cavity outlet pipe 16, and connects with the vapor-phase flow path of carrier gas, and quantitative ring is washed in carrier gas, and the combination gas during promotion quantitatively encircles enters gas chromatograph analysis.
Described oxygen chamber admission line 7, oxygen chamber outlet pipe 8, carrier gas cavity outlet pipe 16, pick-up unit pipeline 18, oxygen channel III5, oxygen channel I 1, carrier gas channel III13 and carrier gas channel I 9 are tracheae.
Oxygen flow is read by 4 controls of oxygen flow variable valve and by oxygen flow meter 6.Carrier gas flux is read by 12 controls of carrier gas flux variable valve and by carrier gas flux meter 14.Part oxygen can permeate under the effect of sample both sides oxygen concentration difference and enter carrier gas cavity 24 by sample 22, and remaining oxygen is then by outlet pipe 8 emptyings of oxygen chamber.Carrier gas enters carrier gas cavity 24 by carrier gas channel I 9, carrier gas flux variable valve 12, carrier gas channel III13, carrier gas flux meter 14, carrier gas cavity admission line 15, and the oxygen of infiltration by sample is carried in carrier gas becomes sample gas, and quantitative sampling device is with volume V
2Carrier gas and the mixed gas of oxygen send into gas chromatograph, detect amount of oxygen in the mixed gas by gas chromatograph.
Embodiment 3
In the present embodiment, the sample of test is an artificial cornea, uses the sample clamp as Fig. 3, and oxygen chamber 21 is the plane with the chamber wall joint of carrier gas cavity 24.All the other are identical with embodiment 2, repeat no more.
Claims (8)
1. the method for testing of an oxygen permeation of cornea repair material is characterized in that, may further comprise the steps:
(1) in gas chromatograph, advances volume V
1Oxygen, from spectrogram, obtain peak area S
1
(2) logical respectively oxygen in the both sides of testing sample and carrier gas, oxygen is penetrated into carrier gas one side through the zone that sample is exposed to Oxygen Flow, with volume V
2Oxygen and the mixed gas of carrier gas send into gas chromatograph, detect the amount of oxygen in the mixed gas, from spectrogram, obtain peak area S
2
(3) the oxygen permeability coefficient Dk of sample, oxygen permeating amount Dk/t can calculate by following formula:
Dk＝(V
1×S
2×t×Q)/(S
1×A×p×V
2)
Dk/t＝(V
1×S
2×Q)/(S
1×A×p×V
2)
Wherein, Dk is an oxygen permeability coefficient, and unit is mlcmcm
-2S
-1Pa
-1Dk/t is an oxygen permeating amount, and unit is mlcmcm
-3S
-1Pa
-1V
1Be the volume of purity oxygen standard specimen, unit is ml; S
1It is the gas chromatogram peak area of purity oxygen standard specimen; T is the harmonic average thickness that sample is exposed to the zone in the Oxygen Flow, and unit is cm; Q is the flow of carrier gas, and unit is mls
-1A is the area that sample is exposed to the zone of Oxygen Flow, and unit is cm
2P is that sample both sides oxygen pressure is poor, and unit is Pa, and its value equals atmospheric pressure; V
2Be the volume of the mixed gas of carrier gas and oxygen, unit is ml; S
2It is the peak area of sending into the oxygen in the mixed gas of gas chromatograph.
2. method of testing according to claim 1 is characterized in that described carrier gas comprises nitrogen, hydrogen or helium.
3. realize the device of the method for testing of the described a kind of oxygen permeation of cornea repair material of claim 1, it is characterized in that, mainly comprise sample clamp (20), oxygen admission gear, carrier gas admission gear and pick-up unit, it is characterized in that described sample clamp (20) is made up of oxygen chamber (21) and carrier gas cavity (24); Oxygen chamber (21) is connected with the oxygen admission gear by oxygen chamber admission line (7), and the oxygen chamber also is provided with oxygen chamber outlet pipe (8); Carrier gas cavity (24) is connected with the carrier gas admission gear by carrier gas cavity admission line (15), and links to each other with pick-up unit by carrier gas cavity outlet pipe (16); Described pick-up unit comprises quantitative sampling device (17) and gas chromatograph (19).
4. device according to claim 3 is characterized in that, described oxygen admission gear comprises oxygen flow meter (6), oxygen channel III (5), oxygen flow variable valve (4), oxygen channel I (1) and the welding bottle that connects successively.
5. device according to claim 3 is characterized in that, described carrier gas admission gear comprises carrier gas flux meter (14), carrier gas channel III (13), carrier gas flux variable valve (12), carrier gas channel I (9) and the carrier gas bottle that connects successively.
6. device according to claim 3 is characterized in that, described oxygen chamber (21) is connected with screw with the chamber wall joint of carrier gas cavity (24).
7. device according to claim 6 is characterized in that, the sample that is positioned at the chamber wall joint of described oxygen chamber (21) and carrier gas cavity (24) is provided with O-ring seal (23) or applies vacuum silica gel.
8. device according to claim 3 is characterized in that, outlet side, described oxygen chamber communicates with the external world by oxygen chamber outlet pipe (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010161752A CN101832982A (en) | 2010-04-26 | 2010-04-26 | Method and device for testing oxygen permeation of cornea repair material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010161752A CN101832982A (en) | 2010-04-26 | 2010-04-26 | Method and device for testing oxygen permeation of cornea repair material |
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| Publication Number | Publication Date |
|---|---|
| CN101832982A true CN101832982A (en) | 2010-09-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010161752A Pending CN101832982A (en) | 2010-04-26 | 2010-04-26 | Method and device for testing oxygen permeation of cornea repair material |
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| Country | Link |
|---|---|
| CN (1) | CN101832982A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103674829A (en) * | 2013-11-25 | 2014-03-26 | 温州医科大学附属眼视光医院 | Measuring method and measuring platform for biomechanical performance parameters of in vitro corneas |
| CN105929078A (en) * | 2016-06-12 | 2016-09-07 | 广州市朴道联信生物科技有限公司 | Oxygen permeability test device and oxygen permeability test method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3494174A (en) * | 1968-01-30 | 1970-02-10 | Varian Associates | Gas chromatography apparatus |
| JPS63172942A (en) * | 1987-01-13 | 1988-07-16 | Ueno Hiroshi | Measuring instrument for gas permeability |
-
2010
- 2010-04-26 CN CN201010161752A patent/CN101832982A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3494174A (en) * | 1968-01-30 | 1970-02-10 | Varian Associates | Gas chromatography apparatus |
| JPS63172942A (en) * | 1987-01-13 | 1988-07-16 | Ueno Hiroshi | Measuring instrument for gas permeability |
Non-Patent Citations (7)
| Title |
|---|
| 《JOURNAL OF APPLIED POLYMER SCIENCE》 19761231 D.G.PYE等 Measurement of Gas Permeability of Polymers.II. Apparatus for Determination of Permeabilities of Mixed Gases and Vapors 文章第287页第2段-第293页倒数第2段、图1 1-8 第20卷, * |
| 《Journal of Membrane Science》 19861215 K.C. O"Brien等 A new technique for the measurement of multicomponent gas transport through polymeric films 229-238 1-8 第29卷, 第3期 2 * |
| 《中国塑料》 19910630 肖惠宁等 聚乙烯醇缩甲醛透氧性能与其结构关系的研究 文章摘要、实验部分第3节、图1 1-8 第5卷, 第2期 2 * |
| 《电子科技大学学报》 19901031 金懋昌 聚合物膜气体渗透率测量的新方法 509-512 1-8 第19卷, 第5期 2 * |
| 《电源技术（研究与设计）》 20080331 罗晓宽等 质子交换膜燃料电池模压石墨双极板研究 174-176 1-8 第32卷, 第3期 2 * |
| D.G.PYE等: "Measurement of Gas Permeability of Polymers.II. Apparatus for Determination of Permeabilities of Mixed Gases and Vapors", 《JOURNAL OF APPLIED POLYMER SCIENCE》 * |
| R.A.PASTERNAK等: "A Dynamic Approach to Diffusion and Permeation Measurements", 《JOURNAL OF POLYMER SCIENCE》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103674829A (en) * | 2013-11-25 | 2014-03-26 | 温州医科大学附属眼视光医院 | Measuring method and measuring platform for biomechanical performance parameters of in vitro corneas |
| CN105929078A (en) * | 2016-06-12 | 2016-09-07 | 广州市朴道联信生物科技有限公司 | Oxygen permeability test device and oxygen permeability test method |
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Application publication date: 20100915 |