CN101809170B - 用于检测核酸的双寡核苷酸方法 - Google Patents

用于检测核酸的双寡核苷酸方法 Download PDF

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Publication number
CN101809170B
CN101809170B CN200880109213.0A CN200880109213A CN101809170B CN 101809170 B CN101809170 B CN 101809170B CN 200880109213 A CN200880109213 A CN 200880109213A CN 101809170 B CN101809170 B CN 101809170B
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oligonucleotide
nucleotide sequence
oligonucleotides
nucleotide
nucleic acid
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CN200880109213.0A
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CN101809170A (zh
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耶西·D·米勒
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3M Innovative Properties Co
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3M Innovative Properties Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200880109213.0A 2007-09-28 2008-09-26 用于检测核酸的双寡核苷酸方法 Expired - Fee Related CN101809170B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US97626007P 2007-09-28 2007-09-28
US60/976,260 2007-09-28
PCT/US2008/077831 WO2009042851A1 (en) 2007-09-28 2008-09-26 Dual oligonucleotide method of nucleic acid detection

Publications (2)

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CN101809170A CN101809170A (zh) 2010-08-18
CN101809170B true CN101809170B (zh) 2014-08-20

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US (2) US8313931B2 (enExample)
EP (1) EP2209909B1 (enExample)
JP (1) JP5432154B2 (enExample)
CN (1) CN101809170B (enExample)
AT (1) ATE505562T1 (enExample)
BR (1) BRPI0816047A2 (enExample)
CA (1) CA2700532A1 (enExample)
DE (1) DE602008006254D1 (enExample)
WO (1) WO2009042851A1 (enExample)

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WO2011029034A2 (en) * 2009-09-04 2011-03-10 Intelligent Medical Devices, Inc. Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of vancomycin resistance genes and vancomycin resistant enterococci
WO2011116318A1 (en) * 2010-03-19 2011-09-22 The Translational Genomics Research Institute Methods, kits, and compositions useful in selecting an antibiotic to treat mrsa
US9074243B2 (en) * 2012-07-27 2015-07-07 New England Biolabs, Inc. Detection of amplification products
EP2948566B1 (en) 2013-01-24 2025-03-26 California Institute of Technology Chromophore-based characterization and detection methods
US10077475B2 (en) * 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
CN103045733B (zh) * 2013-02-05 2014-05-14 中国农业科学院油料作物研究所 无探针双引物标记交互荧光能量共振转移定量pcr方法
GB2512631A (en) * 2013-04-03 2014-10-08 Rupert Maxwell Gaut Quantitative detection of specific nucleic acid sequences
US12104205B2 (en) 2017-06-20 2024-10-01 Mgi Tech Co., Ltd. PCR primer pair and application thereof
CN110612355B (zh) * 2017-06-20 2024-01-12 深圳华大智造科技股份有限公司 用于定量pcr扩增的组合物及其应用
EP3643787A4 (en) 2017-06-20 2021-01-06 MGI Tech Co., Ltd. PCR PRIMER PAIR AND USE OF IT
EP4021474B1 (en) 2019-08-28 2024-12-25 Contamination Source Identification Inc Rapid isolation and collection of microbial rna from a biological specimen

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EP1726664A1 (en) * 2005-05-28 2006-11-29 KBiosciences Ltd. Detection system for PCR assay
CN1940087A (zh) * 2006-08-18 2007-04-04 上海科华生物工程股份有限公司 一种同步扩增检测肝炎及艾滋病毒核酸的方法及试剂盒
EP1780291A1 (en) * 2006-03-01 2007-05-02 Institut Biokhimii I Genetiki Ufimskogo Nauchnogo Tsentra Ran Method of detecting specific fragments of DNA or RNA with the aid of a polymerase chain reaction
CN101017141A (zh) * 2006-02-09 2007-08-15 港龙生物技术（深圳）有限公司 诊断人类乳头状病毒（hpv）感染的荧光聚合酶链式反应（pcr）的方法和试剂盒
WO2007060707A3 (en) * 2005-11-23 2007-10-18 Diasorin Spa Reagents and method for simultaneous nucleic acid amplification and detection

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US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5750666A (en) 1988-05-26 1998-05-12 Competitve Technologies, Inc. Polynucleotide phosphorodithioate compounds
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
WO1992005186A1 (en) 1990-09-20 1992-04-02 Gilead Sciences Modified internucleoside linkages
WO1993009128A1 (en) 1991-11-07 1993-05-13 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
EP1493826B1 (en) 1996-06-04 2008-03-26 University Of Utah Research Foundation Container for carrying out and monitoring biological processes
WO1997046714A1 (en) 1996-06-04 1997-12-11 University Of Utah Research Foundation Monitoring hybridization during pcr
JP3016759B2 (ja) * 1997-02-28 2000-03-06 スミスクライン・ビーチャム・コーポレイション 競争的ハイブリダイゼーションによる蛍光エネルギー転移
US6361942B1 (en) * 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
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US6627159B1 (en) 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
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US7192560B2 (en) 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
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CN1548545A (zh) * 2003-05-14 2004-11-24 宇 王 一种用于多靶序列pcr检测的公共引物及其对多靶核酸序列进行pcr扩增的方法
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EP1726664A1 (en) * 2005-05-28 2006-11-29 KBiosciences Ltd. Detection system for PCR assay
WO2007060707A3 (en) * 2005-11-23 2007-10-18 Diasorin Spa Reagents and method for simultaneous nucleic acid amplification and detection
CN101017141A (zh) * 2006-02-09 2007-08-15 港龙生物技术（深圳）有限公司 诊断人类乳头状病毒（hpv）感染的荧光聚合酶链式反应（pcr）的方法和试剂盒
EP1780291A1 (en) * 2006-03-01 2007-05-02 Institut Biokhimii I Genetiki Ufimskogo Nauchnogo Tsentra Ran Method of detecting specific fragments of DNA or RNA with the aid of a polymerase chain reaction
CN1940087A (zh) * 2006-08-18 2007-04-04 上海科华生物工程股份有限公司 一种同步扩增检测肝炎及艾滋病毒核酸的方法及试剂盒

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Also Published As

Publication number Publication date
EP2209909A1 (en) 2010-07-28
JP5432154B2 (ja) 2014-03-05
US20100203537A1 (en) 2010-08-12
CN101809170A (zh) 2010-08-18
US8652812B2 (en) 2014-02-18
US8313931B2 (en) 2012-11-20
US20130171635A1 (en) 2013-07-04
EP2209909B1 (en) 2011-04-13
CA2700532A1 (en) 2009-04-02
JP2010539940A (ja) 2010-12-24
BRPI0816047A2 (pt) 2018-06-05
DE602008006254D1 (de) 2011-05-26
WO2009042851A1 (en) 2009-04-02
ATE505562T1 (de) 2011-04-15

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