CN101805766A - Process for extracting active polysaccharide - Google Patents
Process for extracting active polysaccharide Download PDFInfo
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- CN101805766A CN101805766A CN 201010166449 CN201010166449A CN101805766A CN 101805766 A CN101805766 A CN 101805766A CN 201010166449 CN201010166449 CN 201010166449 CN 201010166449 A CN201010166449 A CN 201010166449A CN 101805766 A CN101805766 A CN 101805766A
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- macroporous resin
- enzymolysis
- polysaccharide
- extraction process
- nanofiltration membrane
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a process for extracting active polysaccharide, which comprises the steps of carrying out enzymolysis by cellulose, separation by macroporous resin, film concentration, freezing and drying on blueberry fresh fruits as a raw material to obtain a finished product. The invention is used for producing the active polysaccharide, has the advantages of high yield, low energy consumption, low cost, high production capacity and high purity and simultaneously retains the flavor of polysaccharide.
Description
Technical field:
The present invention relates to a kind of technology of extracting active polysaccharide, especially a kind of method of from blueberry, extracting polysaccharide.
Background technology:
Since the eighties, scientists is to vegetable polysaccharides, particularly research has produced keen interest to the polysaccharide in the Chinese medicine, so far reported in succession that kind more than 100 has the herbal polysaccharide of immunomodulatory, multiple physiologically active such as antitumor, antiviral, anti-infective, hypoglycemic, have in clinical assisting therapy and the rehabilitation that is used for tumour, hepatitis, disease such as cardiovascular, wherein most important pharmacological action is when pushing away immunologic enhancement.Existing a large amount of pharmacology and clinical application show, the polysaccharide that these functions are definite, its crude drug in whole belong to help class Chinese medicine mostly.Recent pharmacological research and clinical application show that all it has significant immuno-potentiation, and its effective constituent is polysaccharide or glycoconjugate glycoconjugates, comprise that different its immunocompetent manifestation of glycopeptide such as glycopeptide protein-polysaccharide is also inequality, have to show as cellular immune function strong, have to show as humoral immune function strong, and have also have a significant lipoid peroxidization resistant, the structural analysis that suppresses these glycopeptide compounds of oxidation of LDL shows, their common feature is the polysaccharide content height, what have can have its general character up to 90% sugared structure, promptly all be terminal with Ara, but also each tool individual character, the main chain that has is that 1-6 links, and has plenty of 1-4 and links, have also in conjunction with pigment.Pharmacological experiments shows also in these glycopeptide compounds that not only overall molecule has immunocompetence, and the polysaccharide chain in the molecule also has immunocompetence, and the effect of the ratio overall molecule that has also is eager to excel.Antitumour drug is because people are dissatisfied to current cancer chemotherapeutic drug as antitumour drug with polysaccharide.Though chemists have synthesized the numerous compound that cancer cells is had quite strong lethal effect, but injuring normal cell too in kill cancer cell, show a lot of side effects clinically, particularly chemotherapeutics causes damage to patient's immunity system, so Polysaccharide Immunoregulators is very attractive on tumor treatment.
Blueberry (Blueberry) formal name used at school blueberry, another name have sincere this, pout have a liking for, all persimmon, pasture fruit etc., belong to Ericaceae genus vaccinium plant.It is blue that fruit is, and drape layer white fruit powder, the pulp exquisiteness, and fruity is sour-sweet, and unique flavor has salubrious pleasant fragrance, can eat raw, also can be processed into nectar and fruit wine.
Blueberry has abundant polysaccharide content, and the about 6g/100g fresh weight of oligomeric polysaccharide wherein, and the about 4.6g/100g fresh weight of content of cellulose can be used as an important source of vegetable polysaccharides.
Summary of the invention:
The objective of the invention is for a kind of product yield height is provided, energy consumption is few, and cost is low, and output is big, and purity of polysaccharide reaches more than 97%, keeps original local flavor, is easy to realize industrialized polysaccharide extracting process.
Preparation method proposed by the invention is as follows:
1) enzymolysis: get blueberry fresh fruit, add 0.5 times of amount of its weight deionized water, add cellulase and hydrochloric acid, intensification enzymolysis 10～12h makes its enzymolysis, filter enzymolysis solution;
2) macroporous resin adsorption: enzymolysis solution sees through the polar macroporous resin absorption of liquid through the non-polar macroporous resin removal of impurities, uses ethanol elution again;
3) membrane concentration: with the elutriant membrane concentration, must hold back concentrated solution with nanofiltration membrane;
4) drying: with the concentrated solution lyophilize, promptly.
Said extracted technology is characterized in that in the described enzymolysis process that the content of cellulase in deionized water is 0.5～3.0%, and regulating pH with hydrochloric acid is 4, and temperature is controlled at 40～45 ℃.
Said extracted technology is characterized in that described non-polar macroporous resin post can use D101, PSD, HZ802 macroporous resin.
Said extracted technology is characterized in that described polar macroporous resin can use LSA-5B, D4020, D201 macroporous resin.
Said extracted technology is characterized in that after the described polar macroporous resin absorption 50～60% ethanol elutions with 3～4 times of amount column volumes.
Said extracted technology is characterized in that described nanofiltration membrane adopts ESNA series nanofiltration membrane or HNF-4040 series nanofiltration membrane.
In sum, there is following advantage in the present invention: enzymolysis step makes extraction yield higher than general solvent extraction, has improved the yield of product and the output under the same raw materials cost; Macroporous resin and membrane sepn all make the treatment condition milder, the intact reservation of glycocalix, and regeneration is simultaneously saved a large amount of energy consumptions and cost easily; The lyophilize step has improved the purity of finished product.
Further specify the present invention below in conjunction with embodiment, but the scope of protection of present invention is not limited to following embodiment.
Embodiment:
Embodiment 1:
Get 100g blueberry fresh fruit (total sugar content 10.3%), be dissolved in the 50L deionized water, add the 2.5g cellulase, add hydrochloric acid and regulate pH to 4, after extracting 10h under 40 ℃ of temperature, enzymolysis solution is crossed the D-101 type macroporous resin column removal of impurities of column volume 5L, must see through liquid, then by LSA-5B type macroporous resin column absorption polysaccharide molecule, then with 15L 50% ethanol elution polysaccharide, with ESNA2 nanofiltration membrane concentrate eluant, concentrated solution lyophilize, finally obtain 8.06g content and be 98.9% polysaccharide finished product.
Embodiment 2:
Get 100g blueberry fresh fruit (total sugar content 9.9%), be dissolved in the 50L deionized water, add the 4.1g cellulase, add hydrochloric acid and regulate pH to 4, after extracting 11h under 42 ℃ of temperature, enzymolysis solution is crossed the PSD macroporous resin column of column volume 5L and is carried out removal of impurities, must see through liquid, adsorbs polysaccharide molecule by D4020 type macroporous resin column then, then with 15L 55% ethanol elution polysaccharide, with HNF40-4040 nanofiltration membrane concentrate eluant, concentrated solution lyophilize, finally obtain 8.25g content and be 99.1% polysaccharide finished product.
Embodiment 3:
Get 100g blueberry fresh fruit (total sugar content 10.4%), be dissolved in the 50L deionized water, add the 9.3g cellulase, add hydrochloric acid and regulate pH to 4, after extracting 1h under 45 ℃ of temperature, enzymolysis solution is crossed the HZ802 macroporous resin column of column volume 5L and is carried out removal of impurities, must see through liquid, adsorb polysaccharide molecule by D201 type macroporous resin column then, then with 15L 60% ethanol elution polysaccharide, with HNF70-4040 nanofiltration membrane concentrate eluant, concentrated solution lyophilize, finally obtain 8.27g content and be 98.6% polysaccharide finished product.
Claims (6)
1. technology of extracting active polysaccharide is characterized in that being made up of following steps:
1) enzymolysis: get blueberry fresh fruit, add 0.5 times of amount of its weight deionized water, add cellulase and hydrochloric acid, intensification enzymolysis 10～12h makes its enzymolysis, filter enzymolysis solution;
2) macroporous resin adsorption: enzymolysis solution sees through the polar macroporous resin absorption of liquid through the non-polar macroporous resin removal of impurities, uses ethanol elution again;
3) membrane concentration: with the elutriant membrane concentration, must hold back concentrated solution with nanofiltration membrane;
4) drying: with the concentrated solution lyophilize, promptly.
2. according to the described extraction process of claim 1, it is characterized in that in the described enzymolysis process that the content of cellulase in deionized water is 0.5～3.0%, regulating pH with hydrochloric acid is 4, and temperature is controlled at 40～45 ℃.
3. according to the described extraction process of claim 1, it is characterized in that described non-polar macroporous resin post can use HZ802, D101, PSD macroporous resin.
4. according to the described extraction process of claim 1, it is characterized in that described polar macroporous resin can use LSA-5B, D4020, D201 macroporous resin.
5. according to the described extraction process of claim 1, it is characterized in that after the described polar macroporous resin absorption 50～60% ethanol elutions with 3～4 times of amount column volumes.
6. according to the described extraction process of claim 1, it is characterized in that described nanofiltration membrane adopts ESNA series nanofiltration membrane or HNF-4040 series nanofiltration membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010166449 CN101805766A (en) | 2010-04-05 | 2010-04-05 | Process for extracting active polysaccharide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010166449 CN101805766A (en) | 2010-04-05 | 2010-04-05 | Process for extracting active polysaccharide |
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| CN101805766A true CN101805766A (en) | 2010-08-18 |
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| CN 201010166449 Pending CN101805766A (en) | 2010-04-05 | 2010-04-05 | Process for extracting active polysaccharide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337315A (en) * | 2011-10-08 | 2012-02-01 | 江苏世东野宝植物资源开发有限公司 | Method for preparing decolorized blueberry polysaccharide |
| CN108048504A (en) * | 2017-12-08 | 2018-05-18 | 福建省农业科学院果树研究所 | A kind of extracting method of Moringa polysaccharide |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101229316A (en) * | 2008-01-31 | 2008-07-30 | 广东药学院 | Rhizoma anemarrhenae extrac and applications as type 2 diabetes-curing medicine thereof |
-
2010
- 2010-04-05 CN CN 201010166449 patent/CN101805766A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101229316A (en) * | 2008-01-31 | 2008-07-30 | 广东药学院 | Rhizoma anemarrhenae extrac and applications as type 2 diabetes-curing medicine thereof |
Non-Patent Citations (2)
| Title |
|---|
| 《食品工业科技》 20091231 孟宪军 酶法优化蓝莓多糖的提取工艺 185-187 1-6 第30卷, 第3期 * |
| 《食品研究与开发》 20091130 朱金艳 蓝莓多糖的提取工艺 103-105 1-6 第30卷, 第11期 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337315A (en) * | 2011-10-08 | 2012-02-01 | 江苏世东野宝植物资源开发有限公司 | Method for preparing decolorized blueberry polysaccharide |
| CN108048504A (en) * | 2017-12-08 | 2018-05-18 | 福建省农业科学院果树研究所 | A kind of extracting method of Moringa polysaccharide |
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Application publication date: 20100818 |