CN101797265A - Application of belamcanda chinensis total isoflavone or isoflavone compounds in preparing medicaments and food for preventing and treating women's disease - Google Patents
Application of belamcanda chinensis total isoflavone or isoflavone compounds in preparing medicaments and food for preventing and treating women's disease Download PDFInfo
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Abstract
The invention relates to the technical field of medicaments, in particular to application of 12 isoflavone compound monomers comprising belamcanda chinensis total isoflavone or tecoridin, wild tectoridin, tectorigenin, iridin and the like in preparing medicaments and food for preventing and treating lower women's estrogen level and retarding womb and ovarian failure or climacteric syndrome. Animal experiment results show that the total isoflavone or 12 isoflavone compound monomers in the belamcanda chinensis have remarkable pharmacological effects of improving the estrogen level, promoting the growth of follicle, promoting epitheliosis of womb and vagina, retarding womb and ovarian failure, improving the expression of an epithelial cell estrogen receptor of the vagina and the like, so the total isoflavone or 12 isoflavone compound monomers can be used for preparing the medicaments or food for preventing and treating lower women's estrogen level and retarding womb and ovarian failure or climacteric syndrome. The application provides a new source for seeking a plant estrogen medicament.
Description
Technical field
The present invention relates to medical technical field, be total isoflavone or 12 the isoflavonoid monomer iridins among Chinese medicine Rhizoma Belamcandae Belamcanda chinensis (L.) DC., the wild flag aglycon, tectorigenin, irisflorentin, iridin, nonirisflorentin, 5,6,7,3 '-tetrahydroxy-4 '-the methoxyl group isoflavone, 5,7-dihydroxy-6,3 ', 4 ', 5 '-the tetramethoxy isoflavone, 5,7,3 '-trihydroxy-8,4 ', 5 '-the trimethoxy isoflavone, 5,7,4 '-trihydroxy-6,3 ', 5 '-the trimethoxy isoflavone, iris aglycone-4 '-glucoside, 5,3 ', 5 '-trimethoxy-6,7-methylene-dioxy isoflavone-4 '-that O-β-D-glucose is used for preparation control women estrogen level is low, the purposes of slowing down the decline of uterus and ovary or preventing and treating the medicine or the food of climacteric syndrome.
Background technology
Rhizoma Belamcandae is the dry rhizome of Iridaceae Rhizoma Belamcandae platymiscium Rhizoma Belamcandae Belamcanda chinensis (L.) DC..The isoflavonoid that the Rhizoma Belamcandae of having reported contains is mainly iridin, the wild flag aglycon, tectorigenin, irisflorentin, iridin, nonirisflorentin, 5,6,7,3 '-tetrahydroxy-4 '-the methoxyl group isoflavone, 5,7-dihydroxy-6,3 ', 4 ', 5 '-the tetramethoxy isoflavone, 5,7,3 '-trihydroxy-8,4 ', 5 '-the trimethoxy isoflavone, 5,7,4 '-trihydroxy-6,3 ', 5 '-the trimethoxy isoflavone, iris aglycone-4 '-glucoside, 5,3 ', 5 '-trimethoxy-6,7-methylene-dioxy isoflavone-4 '-O-β-[Jin Li such as D-glucose, Chen Hai-Sheng et al.New flavone and isoflavone glycoside from Belamcandachinensis, Chinese Chemical Letters 2007,18:158-160; Jin Li, Chen Hai-sheng, Jin Yong-Sheng, Liang Shuang, Liu Jian-Guo Chemical constituents fromBelamcanda chinensis Journal of Asian Natural Products Research 2008,10 (1): 89-91; Hu Xiaolan, Xu's posthumous title, Huang Tianxia etc., the separation of chemical constituent and evaluation in the Rhizoma Belamcandae, bulletin of Chinese materia medica, 1982,7 (1): 29～30; Propitious essay is bright, Qin Minjian, Wang Zhengtao. the chemical constitution study of Rhizoma Belamcandae (I), China Medicine University's journal, 2001,32 (3): 197～199; Surplus subclass, Wang Conghui, Liu Dai etc., the research of lipophilic ingredients in the B.Chinensis rhizome, Acta Pharmaceutica Sinica, 1983,18 (12): 969～972; Yamaki M, et al., Planta Med., 1990,56 (3): 335; Hydeyuki Ito, Satomi Onoue, et al., Isoflavonoids from Belamcanda chinensis, Chem.Pharm.Bull, 2001,49 (9): 1229～1231; Zhou Lixin, Lin Mao, He Lanfeng, the chemical constitution study of Rhizoma Belamcandae (I), Chinese herbal medicine, 1996,27 (1): 8～10; Qin Minjian, propitious essay is bright, Wang Zhengtao, the chemical constitution study of Rhizoma Belamcandae (II), Chinese herbal medicine, 2004,35 (5): 487～489; Won Sick Woo, Eun Hee Woo, An Isoflavone Noririsflorentin from Belamcanda chinensis, Phytochemistry, 1993,33 (4): 939～940; Zhou Lixin etc., Chinese chemical wall bulletin, 1997,8 (2): 133].
Their chemical structure of general formula is:
Wherein, R
1Be OH or OCH
3
R
2Be OCH
3, OH or H;
R
3Be OH or Oglu;
R
4Be OCH
3Or H;
R
5Be OCH
3, OH or H;
R
6Be OCH
3, OH or Oglu;
R
7Be OCH
3Or H;
The group collocation of each chemical compound sees Table 1.
The group collocation of table 112 isoflavonoid
So far do not see that belamcanda chinensis total isoflavone or above-mentioned 12 isoflavonoids have the report of the low or climacteric syndrome function of control women estrogen.
Summary of the invention
The objective of the invention is provides a kind of control women estrogen low or the medicine of climacteric syndrome or purposes of food of preparing to isoflavonoid such as iridin, wild flag aglycon, tectorigenin, irisflorentin, iridin and belamcanda chinensis total isoflavone.
Through zoopery, 12 isoflavonoids such as belamcanda chinensis total isoflavone or iridin, wild flag aglycon, tectorigenin, iridin can improve estrogen level to female naturally-aged rat of 12～15 monthly age SD or castrated rats, promote follicle to generate, promote uterus, vagina epithelium hypertrophy, slow down the decline of uterus and ovary, also can improve the expression of vaginal epithelial cell estrogen receptor, therefore, can be used for preparing the medicine or the food of the low or climacteric syndrome of control women estrogen level.
The specific embodiment
Now in conjunction with the embodiments, the present invention is further described.
Embodiment 1. preparation belamcanda chinensis total isoflavones
The dry medical material 10Kg of Rhizoma Belamcandae extracts three times with 10 times of amount 80% ethanol water heat routinely, and each extraction time is 100 minutes, filter, merging filtrate, decompression recycling ethanol is behind thin up to the 10 times volume, go up the AB-8 macroporous resin column routinely, with 5 bed volumes of water elution, with 5 bed volumes of 90% ethanol water eluting, collect this 90% ethanol elution more earlier, decompression recycling ethanol, concentrate drying gets belamcanda chinensis total isoflavone 198g, the dissolving of 30 times of amounts of total isoflavone reuse, 80% alcohol heating reflux, filter, filtrate decompression is concentrated into about 1/8th volumes, and concentrated solution is placed the precipitation of spending the night, filtering-depositing, drying gets the belamcanda chinensis total isoflavone product.Through assay, the content of osajin composition (in iridin, tectorigenin, wild flag aglycon, irisflorentin, five main constituents of iridin) is greater than 91%.
The preparation of 12 isoflavone compounds such as embodiment 2. iridins, wild flag aglycon, tectorigenin, iridin (sees Jin Li (Jin Li) for details, Chen Hai-sheng (Chen Haisheng), et al.Chemicalconstituents from Belamcanda chinensis Journal of Asian Natural ProductsResearch 2008,10 (1): 89-91)
Chinese medicine Rhizoma Belamcandae dry rhizome 4Kg, pulverize the back with 80% ethanol water heating and refluxing extraction 3 times, decompression and solvent recovery, get the about 200g of fluid extract, it is scattered among the distilled water 800ml, extract (each 500ml) respectively 3 times with petroleum ether, chloroform and ethyl acetate successively, after reclaiming solvent respectively, obtain petroleum ether extract 22g, chloroform extract 65g, acetic acid ethyl ester extract 28g.
Get chloroform extract 65g, carry out silica gel column chromatography gradient solvent system: CHCl
3→ CHCl
3/ CH
3OH: H
2O (20: 1) obtains stream part C1, C2 and C3 respectively.The C1 part is carried out the SephadexLH20 column chromatography again, use CHCl
3/ CH
3OH (1: 1) eluting is collected respective streams part, obtains compd B C-2 respectively, BC-4, BC-5, BC-6 and BC-10.The C2 part is carried out Sephadex LH20 column chromatography again, use CHCl
3/ CH
3OH (1: 1) eluting is collected respective streams part, obtains compd B C-3 respectively, BC-8, BC-9.
Get acetic acid ethyl ester extract 28g through silica gel column chromatography gradient solvent system: CHCl
3/ CH
3OH (20: 1 → 1: 1) obtains stream part E1, E2 and E3.The E3 part is carried out Sephadex LH20 column chromatography again, use CHCl
3/ CH
3OH (1: 1) eluting is collected respective streams part, obtains compd B C-1, BC-7, BC-11 and BC-12
Embodiment 3. chemical compounds are tested naturally-aged rat estrogen-like effects
One, experiment material
(1) laboratory animal
100 of 12～15 monthly age SD female rats are clean animal, and the animal quality certification: SCXK (army) 2007-011 is provided by INM zoopery center.Test preposition animal and conform a week in indoor, the feeding environment temperature is 18～22 ℃, and relative humidity is (50 ± 20) %, freely drinks water.
(2) be subjected to the reagent thing
1, belamcanda chinensis total isoflavone and 4 isoflavonoids: by embodiment 1 preparation
(1) belamcanda chinensis total isoflavone (BC), total isoflavone content 〉=90%.
(2) iridin (BC-1)
(3) wild flag aglycon (BC-2)
(4) tectorigenin (BC-3)
(5) iridin (BC-7
2, positive control medicine:
(1) soybean isoflavone, lot number: 090118, Handan billowing wheat bio tech ltd.
(2) GENGNIANAN PIAN, lot number: 080701, Tianshikang Chinese Medicines Co., Ltd., Jiangxi.
3, the pellet of animal: press rat body weight 100g/kg every day for food, will be subjected to the reagent thing to be processed into pellet by dosage adding rat feed respectively before using, put in 50 ℃ the baking oven and dry.The every 100g medicament contg of different pharmaceutical pellet is respectively:
1) belamcanda chinensis total isoflavone (BC): three dosage of 400mg, 600mg and 800mg correspond respectively to basic, normal, high three dosage groups;
2) iridin (BC-1): 600mg;
3) wild flag aglycon (BC-2): 600mg;
4) tectorigenin (BC-3): 600mg;
5) iridin (BC-7): 600mg;
6) soybean isoflavone: 600mg;
7) GENGNIANAN PIAN: 600mg.
(3) main agents
Serum estradiol (E
2), follicule-stimulating hormone (FSH) (FSH), lutropin (LH) radioimmunoassay kit, Tianjin Jiuding Medical Biological Engineering Co., Ltd provides;
Serum corticosterone integrated enzyme reaction test kit, Diagnostic Systems Laboratories Inc company
Provide; Estrogen receptor (ER) test kit, Shanghai Chang-dao Bioengineering Co., Ltd. provides.
(4) experimental apparatus
The full-automatic two probe radioimmunity γ registers of SN-697, Shanghai nuclear institute day ring photoelectric instrument is limited
Company; 80-2 type centrifuge, Shanghai Precision Scientific Apparatus Co., Ltd;
The FA-N/FC electronic analytical balance, Shanghai Precision Scientific Apparatus Co., Ltd.
Two, experimental technique
(1) grouping: 100 12～15 monthly age SD female rats are divided into 10 groups at random, 10 every group, divide two cages to raise 5 in every cage:.
1. the blank group is pressed the pellet 100g/kg of not dosing of the disposable feeding of body weight every day;
2. belamcanda chinensis total isoflavone low dose group, every day, dosage was 400mg/kg by body weight feeding low dosage BC pellet 100g/kg;
3. dosage group in the belamcanda chinensis total isoflavone, every day, dosage was 600mg/kg by dosage BC pellet 100g/kg in the body weight feeding;
4. belamcanda chinensis total isoflavone high dose group, every day, dosage was 800mg/kg by body weight feeding high dose BC pellet 100g/kg;
5. iridin (BC-1) is organized, and every day, dosage was 600mg/kg by body weight feeding BC-1 pellet 100g/kg;
6. wild flag aglycon (BC-2) is organized, and every day, dosage was 600mg/kg by body weight feeding BC-2 pellet 100g/kg;
7. tectorigenin (BC-3) is organized, and every day, dosage was 600mg/kg by body weight feeding BC-3 pellet 100g/kg;
8. iridin (BC-7) is organized, and every day, dosage was 600mg/kg by body weight feeding BC-7 pellet 100g/kg;
9. soybean isoflavone group, every day, dosage was 600mg/kg by body weight feeding soybean isoflavone pellet 100g/kg;
10. GENGNIANAN PIAN group, every day, dosage was 600mg/kg by body weight feeding GENGNIANAN PIAN pellet 100g/kg.
(2) observe and detect
According to dosage for food, once a day, drinking-water is free, successive administration 30 days with above-mentioned each group.Feed after 30 days, broken end is got blood, and separation of serum is surveyed estradiol (E in the serum
2), follicule-stimulating hormone (FSH) (FSH), lutropin (LH), corticosterone content; Getting uterus, ovary, vagina, adrenal gland weighs, calculate organ coefficient, and be fixed, paraffin embedding, section, HE dyeing, the LeicaDMLB optical microscope carries out to section that tectology is observed, the expression of immunohistochemical staining vaginal epithelial cell estrogen receptor.
1, the mensuration of serum gonadal hormone
Broken end is got blood, separation of serum routinely, and-20 ℃ of preservations are exempted from estradiol (E in the method mensuration serum with putting
2), follicule-stimulating hormone (FSH) (FSH), lutropin (LH) content; Measure corticosterone content with enzyme-linked method; Strict with each medicine box description operation.
Experimental data mean ± standard deviation
Expression adopts paired t-test to make administration front and back statistical analysis, and P＜0.05 is judged as has significant significant difference.The results are shown in Table 1.
Table 1 is respectively organized rat blood serum E
2, FSH, LH, corticosterone level comparison
Annotate: compare * p＜0.05 * * p＜0.01 with the blank group
Usually, Woman climacteric serum E
2Level descends, and serum FSH level and serum Lh level raise, and the serum corticosterone level descends.By table 1 as seen, compare each administration group serum E with the blank group
2Level all has raising in various degree, serum FSH level and serum Lh level all have decline in various degree, the serum corticosterone level raises in various degree to some extent, some index near in addition be better than positive control drug soybean isoflavone or GENGNIANAN, illustrate that said belamcanda chinensis total isoflavone of the present invention or isoflavonoid can prevent and treat or improve the menopausal syndrome symptom.
2, organ coefficient is measured
Dissect and take out uterus, ovary, vagina, adrenal gland, reject the connective tissue and the fat of periphery as far as possible, accurately weigh, be calculated as follows organ coefficient.
Organ coefficient=organ weights (g)/body weight (g) * 1000
Each treated animal sexual organ organ coefficient measurement result sees Table 2.
Table 2 is respectively organized rat sexual organ organ coefficient measurement result relatively
Annotate: compare * p＜0.05 * * p＜0.01 with the blank group
Usually, Woman climacteric uterus, vagina, gonad cell decline, adrenal gland's function reduction.By table 2 as seen, compare with the blank group, each administration group uterus vagina coefficient, ovary coefficient and adrenal gland (left side) coefficient all have increase in various degree, approaching with positive controls, explanation can make gonad cell propagation, uterus quality increase, thereby can improve and prevent and treat the menopausal syndrome symptom.
3, pathologic finding and immunohistochemical experiment
Uterus, ovary, vagina, adrenal gland are used 10% formalin fixed 72 hours respectively routinely, specimens paraffin embedding slices, about 4～5 μ m of slice thick, HE dyeing, the LeicaDMLB observation by light microscope is captured microphotograph under the Olympus DV.And carry out endometrial estrogen receptor immunohistochemical staining, statistics positive expression result is:
1) respectively organizes rat uterus, vagina, ovary, the histological examination of adrenal gland's pathology
From each result of internal organs histopathologic examination of rat, each is organized rat uterus, vagina, ovary, adrenal gland's internal organs and is organized in microscopically and there is no changes such as tangible dysplasia, atrophy, hemorrhage, necrosis and inflammation; The follicle that as seen different development degrees and quantity are arranged in the ovary.Compare the pathologic finding of each group, endometrium, the vagina epithelium of iridin (BC-1), wild flag aglycon (BC-2), Rhizoma Belamcandae isoflavone high dose group has relatively significantly neointimal hyperplasia phenomenon; Iridin (BC-1), wild flag aglycon (BC-2), tectorigenin (BC-3), iridin (BC-7) group follicle quantity relatively significantly increase, the growth and the quantity that can promote follicle in the ovary after the administration are described, make endometrium, vagina epithelium neointimal hyperplasia, can prevent and treat the clinical symptoms that menopausal syndrome causes because of sexual organ's decline.
2) SABC estrogen receptor
Estrogen receptor all has expression at the epithelial cell of each group rat uterus, estrogen receptor is expressed more obvious in iridin (BC-1), wild flag aglycon (BC-2), tectorigenin (BC-3) group, near with the soybean isoflavone winding, explanation can make the uterine estrogen receptor of rat raise, thereby improves the caused symptom of climacteric decrease in estrogen.
4. pairs of castrated rats estrogen-like effects tests of embodiment
One, experiment material
(1) laboratory animal: with embodiment 3
(2) be subjected to the reagent thing: with embodiment 3
1, positive control medicine:
(1) soybean isoflavone, lot number: 090118, Handan billowing wheat bio tech ltd.
(2) GENGNIANAN PIAN, lot number: 080701, Tianshikang Chinese Medicines Co., Ltd., Jiangxi.
(3) chloral hydrate lot number: 20080410 Chemical Reagent Co., Ltd., Sinopharm Group.
2, pellet of animal and medicament contg: with embodiment 3
(3) main agents is with embodiment 3
(4) experimental apparatus is with embodiment 3
Two, experimental technique
(1) rat castration: rat abdominal cavity gives 9% chloral hydrate (10ml/kg) anesthesia.Make the stringer skin otch of 1.0-1.5cm along the back posterior midline, bilateral is isolated ovary respectively, ligation, spay, check no oozing of blood, the recovery organ is sewed up complete, and the outer side injection penicillin (20,000 u/kg) of leg muscle is antibiotic, for three days on end, postoperative is noted insulation, freely drinks water, takes food, and a postoperative 1-6 hour rat all revives.Postoperative the 9th day is divided into 10 groups at random with rat, 10 every group, divides two cages to raise 5 in every cage.
(2) grouping and administration are with embodiment 3.
(3) observe and detect
Respectively by 100g/kg rat body weight administration every day, drinking-water is free, successive administration 30 days with ready-made medicine-containing particle feedstuff.Feed after 30 days, broken end is got blood, and separation of serum is surveyed estradiol (E in the serum
2), follicule-stimulating hormone (FSH) (FSH), lutropin (LH), corticosterone content; Getting uterus, vagina, adrenal gland weighs, calculate organ coefficient, and be fixed, paraffin embedding, section, HE dyeing, the LeicaDMLB optical microscope carries out to section that tectology is observed, the expression of immunohistochemical staining vaginal epithelial cell estrogen receptor.
1, the mensuration of serum gonadal hormone
Broken end is got blood, separation of serum routinely, and-20 ℃ of preservations are exempted from estradiol (E in the method mensuration serum with putting
2), follicule-stimulating hormone (FSH) (FSH), lutropin (LH) content; Measure corticosterone content with enzyme-linked method; Strict with each medicine box description operation.
Experimental data mean ± standard deviation
Expression adopts paired t-test to make statistical analysis before and after the administration, and P＜0.05 is judged as to have significant significant difference and the results are shown in Table 3
Table 3 is respectively organized rat blood serum E
2, FSH, LH, corticosterone level comparison
Annotate: compare * p＜0.05 * * p＜0.01 with the blank group
By table 3 as seen, each is organized castrated rats determination of serum result and compares with the blank group, each administration group E
2Level and positive controls are close to or higher than positive controls, particularly dosage group and iridin tuple in the belamcanda chinensis total isoflavone (BC); The FSH level descends to some extent, wherein dosage group descend significantly (p＜0.01) in the belamcanda chinensis total isoflavone (BC); The LH level all has decline in various degree, particularly iridin (BC-1) and tectorigenin (BC-3); The serum corticosterone level has obvious rising.The result shows that they can be used for improving the menopausal syndrome symptom.
2, organ coefficient is measured
Behind the sacrifice of animal, dissect and take out uterus, vagina, adrenal gland, reject the connective tissue and the fat of periphery as far as possible, accurately weigh, be calculated as follows organ coefficient.
Organ coefficient=organ weights (g)/body weight (g) * 1000
Each treated animal sexual organ organ coefficient measurement result is more as shown in table 4.
Table 4 is respectively organized rat sexual organ organ coefficient measurement result relatively
Annotate: compare * p＜0.05 with the blank group; * p＜0.01
By table 4 as seen, administration group castrated rats uterus vagina coefficient is compared with the blank group, dosage and high dose, iridin (BC-1), wild flag aglycon (BC-2), tectorigenin (BC-3), iridin (BC-7) group uterus vagina coefficient all have increase, particularly tectorigenin (BC-3) group in various degree in the belamcanda chinensis total isoflavone (BC); Adrenal gland (left side) index variation is little.Show the clinical symptoms that to improve and to prevent Woman climacteric to cause.
3, pathologic finding and SABC inspection
Get uterus, vagina, adrenal gland through 10% formalin fixed 72 hours, specimens paraffin embedding slices, about 4～5 μ m of slice thick, HE dyeing, the LeicaDMLB observation by light microscope is captured microphotograph under the Olympus DV.And carry out endometrial estrogen receptor immunohistochemical staining, add up positive expression.
1) respectively organizes castrated rats uterus, vagina, the histological examination of adrenal gland's pathology
From each result of internal organs histopathologic examination of castrated rats, each is organized castrated rats uterus, vagina, adrenal gland's internal organs and is organized in microscopically and there is no changes such as tangible dysplasia, atrophy, hemorrhage, necrosis and inflammation.Compare the pathologic finding of each group, iridin (BC-1), wild flag aglycon (BC-2), tectorigenin (BC-3), iridin (BC-7), belamcanda chinensis total isoflavone high dose group have relatively significantly endometrial hyperplasia phenomenon, the same with the soybean isoflavone group, make endometrium, vagina epithelium neointimal hyperplasia after administration is described, therefore can prevent and treat the clinical symptoms that climacteric syndrome causes because of sexual organ's decline.
2) SABC estrogen receptor
Estrogen receptor all has expression at the epithelial cell in each group castrated rats uterus, expression in estrogen receptor iris aglycone out of office (BC-2), tectorigenin (BC-3), iridin (BC-7), the belamcanda chinensis total isoflavone high dose group is more obvious, consistent with the soybean isoflavone group, illustrate that the administration group can make the uterine estrogen receptor of rat raise, and can prevent and treat climacteric syndrome because the clinical symptoms that decrease in estrogen causes.
Above-mentioned experimental result shows, isoflavonoids such as belamcanda chinensis total isoflavone or iridin, wild flag aglycon, tectorigenin, iridin have the estrogen level of significantly improving, promote follicle to generate, promote uterus, vagina epithelium hypertrophy, slow down the decline of uterus and ovary, and the expression that can improve the vaginal epithelial cell estrogen receptor, these pharmacological actions explanation belamcanda chinensis total isoflavones or isoflavonoid monomer can prevent and treat that the Woman climacteric estrogen level changes and a series of clinical symptoms of causing.Therefore, they can be used for preparing control women estrogen level low, slow down the medicine or the food of uterine cell and vaginal epithelial cell and ovary decline or climacteric syndrome.
Claims (2)
1. isoflavonoid iridin, the wild flag aglycon, tectorigenin, iridin, irisflorentin, nonirisflorentin, 5,6,7,3 '-tetrahydroxy-4 '-the methoxyl group isoflavone, 5,7-dihydroxy-6,3 ', 4 ', 5 '-the tetramethoxy isoflavone, 5,7,3 '-trihydroxy-8,4 ', 5 '-the trimethoxy isoflavone, 5,7,4 '-trihydroxy-6,3 ', 5 '-the trimethoxy isoflavone, iris aglycone-4 '-glucoside or 5,3 ', 5 '-trimethoxy-6,7-methylene-dioxy isoflavone-4 '-O-β-D-glucose is low at preparation control women estrogen level, slow down the medicine or the Application in Food of decline of uterus and ovary or climacteric syndrome
Their chemical structure of general formula is as follows:
Wherein: R
1=OH or OCH
3R
2:=OCH
3, OH or H; R
3=OH or Oglu;
R
4=OCH
3Or H; R
5=OCH
3, OH or H; R
6=OCH
3, OH or Oglu; R
7=OCH
3Or H;
The group collocation of each chemical compound is as follows respectively:
2. belamcanda chinensis total isoflavone is prevented and treated medicine or the Application in Food that women's estrogen level is low, slow down uterus and ovary decline or climacteric syndrome in preparation, and the preparation method of said belamcanda chinensis total isoflavone is as follows:
Get the dry medical material of Rhizoma Belamcandae, extract three times with the 80% ethanol water heat of 10 times of volumes, each extraction time is 100 minutes, filter, decompression filtrate recycling ethanol, behind thin up to the 10 times volume, last AB-8 macroporous resin column, earlier with 5 bed volumes of water elution, with 5 bed volumes of 90% ethanol water eluting, collect this 90% ethanol elution, decompression recycling ethanol again, concentrate drying gets belamcanda chinensis total isoflavone.
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| CN113429442A (en) * | 2021-08-06 | 2021-09-24 | 康美华大基因技术有限公司 | Method for separating tectoridin and tectorigenin from rhizoma Belamcandae water extraction residues |
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| CN102127047A (en) * | 2011-01-10 | 2011-07-20 | 中国药科大学 | Method for separating 5-hydroxyl isoflavonoids monomeric compound in blackberry lily |
| CN102127047B (en) * | 2011-01-10 | 2012-06-27 | 中国药科大学 | Method for separating 5-hydroxyl isoflavonoids monomeric compound in blackberry lily |
| CN110381954A (en) * | 2017-03-07 | 2019-10-25 | 株式会社Lg生活健康 | Menopause anesis composition comprising tectorigenin -7-O- xylose glucose glycosides |
| CN110381954B (en) * | 2017-03-07 | 2023-08-08 | 株式会社Lg生活健康 | Composition for alleviating female climacteric symptoms comprising tectorigenin-7-O-xyloglucan |
| CN106995425A (en) * | 2017-04-20 | 2017-08-01 | 四川省中医药科学院 | The extraction process and its isolation and purification method of a kind of Rhizoma Belamcandae flavone aglycone |
| CN108653729A (en) * | 2018-08-27 | 2018-10-16 | 广州汇高生物科技有限公司 | A kind of vaginal foam agent and its application |
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| CN109010825B (en) * | 2018-08-27 | 2021-11-19 | 广州汇高生物科技有限公司 | Vagina in-situ gel preparation and preparation method and application thereof |
| CN108653729B (en) * | 2018-08-27 | 2021-11-19 | 广州汇高生物科技有限公司 | Vaginal foaming agent and application thereof |
| CN113429442A (en) * | 2021-08-06 | 2021-09-24 | 康美华大基因技术有限公司 | Method for separating tectoridin and tectorigenin from rhizoma Belamcandae water extraction residues |
| CN113730558A (en) * | 2021-09-09 | 2021-12-03 | 广东圆康再生医学科技开发有限公司 | Ovarian oocyte repair composition and preparation method thereof |
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