CN101787354A - Nutritious bacillus subtilis capable of inhibiting diseases and degrading organic phosphorus pesticide - Google Patents
Nutritious bacillus subtilis capable of inhibiting diseases and degrading organic phosphorus pesticide Download PDFInfo
- Publication number
- CN101787354A CN101787354A CN 201010109343 CN201010109343A CN101787354A CN 101787354 A CN101787354 A CN 101787354A CN 201010109343 CN201010109343 CN 201010109343 CN 201010109343 A CN201010109343 A CN 201010109343A CN 101787354 A CN101787354 A CN 101787354A
- Authority
- CN
- China
- Prior art keywords
- subtilis
- bacillus subtilis
- sbm
- degrading organic
- nutrition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention provides a nutritious bacillus subtilis capable of inhibiting diseases and degrading an organic phosphorus pesticide, and relates to a bacillus subtilis. The invention solves the problems that the degrading bacteria of the existing organic phosphorus pesticide have low degradation rate, can not provide nutrients and can not achieve the bacteriostatic effect on crops. The bacillus subtilis SbM-10 of the invention was collected by China General Microbiological Culture Collection Center on January 18th, 2010 with the collection number of 3583. The bacillus subtilis is a bacillus with the length of 2.0-3.0 micrometers and the width of 0.7-0.8 micrometer, is arranged in a chain-like conformation, and has spores but no capsules; and the bacillus subtilis presents with coarseness, opacity, no flashing, overspreading and grey-white color on the beef extract peptone medium. The bacillus subtilis of the invention can provide nutrients and achieve the disease inhibiting effect on the crops, and can well degrade the organic phosphorus pesticide, thereby having wide application range.
Description
Technical field
The present invention relates to a kind of subtilis.
Background technology
Organophosphorus pesticide is the Pesticidal products of domestic and international widespread production and use, is one of important measures that guarantee at present the agricultural year stable yields.At present, acephatemet, parathion-methyl, Rogor, the Malathion, Trichlorphon, SD-1750, omethoate, thiophos, organophosphorus pesticides such as Chlorpyrifos 94, though occupy the leading market of China's agricultural chemicals. country has put into effect related measure restriction acephatemet, thiophos, parathion-methyl, monocrotophos, the production and sales of five kinds of high malicious organophosphorus pesticides such as phosphamidon, but because the long-term excess of organophosphorus pesticide is used, cause the pollution of present organophosphorus pesticide to become one of source of pollution the most serious in the agriculture production environment, studies show that, organophosphorus pesticide has mutagenicity and causes birth defect, mammiferous nerve is relevant with organophosphorus pesticide mostly with disease of immune system, and these diseases comprise mad cow disease, the complexities of the Gulf War, parkinsonism etc.Accumulation migration takes place in organophosphorus pesticide in soil, cause that pesticide residue exceed standard in the agricultural-food, causes food-safety problem, has also restricted the outlet of agricultural products in China.How to eliminate organophosphorus pesticide to environment and human harm, become problem concerning the human health and the national economic development.Therefore press for and set up a kind of effective ways and be used to the pesticide residue of curbing environmental pollution and removing agricultural-food.With conventional chemical (highly basic hydrolysis etc.), physics (photodegradation etc.) degradation method inefficiency, its hydrolysate also is that the pollutent of environment is compared, the microbiological deterioration organophosphorus pesticide has that expense is low, environmental influence is little, can reduce Pollutant levels to greatest extent, degraded thoroughly, can be used for the advantages such as place that other technologies are difficult to use.Microorganism Decompounding of Pesticide is meant the structure of agricultural chemicals is changed, and causes the chemistry of agricultural chemicals and the process that physical properties changes, and by agricultural chemicals is degraded to micromolecular compound from macromolecular cpd, becomes H at last
2O and CO
2, realize innoxious degraded to environment.In actual production, utilize the remains of pesticide in the organophosphorus pesticide degradation bacterium degraded ecotope and the pesticidal contamination on agricultural-food surface, the social concern that can obviously reduce the unnecessary financial loss of peasant and be caused; Utilize biodegradation technique can acceleration environment in the degraded of xenobiotic pollutants, also be simultaneously a kind of economy, efficient and to the cleaning means of ecological environment security.Now become the focus of environment pesticidal contamination improvement work.But existing organophosphorus pesticide degradation bacterium only is about 60% to the degradation rate of organophosphorus pesticide Rogor, to crop alimentary, press down sick effect and study, and function singleness, the practical application effect shakiness has limited the use of organophosphorus pesticide degradation bacterium greatly.And the subtilyne that produces in the thalli growth process of existing bacillus subtilis strain, polymyxin, nystatin, linear gramicidins isoreactivity material, these active substances have the obvious suppression effect to the conditioned pathogen of pathogenic bacterium or autogenous infection.But the bacillus subtilis bacterial strain that has the machine phosphorus insecticide degradation effect does not at present appear in the newspapers.
Summary of the invention
The objective of the invention is to possess nutrition in order to provide a kind of, press down sick effect, and organophosphorus pesticide is had the subtilis of high degradation rate.
The present invention has nutrition, presses down the subtilis SbM-10 (Bacillus subtilis SbM-10) of disease and degrading organic phosphor pesticides, in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCC No.3583, and preservation date is on 01 18th, 2010; Subtilis SbM-10 is shaft-like, and length is 2.0～3.0 microns, and wide is 0.7～0.8 micron, catenation, and no pod membrane has gemma; Coarse on beef-protein medium, opaque, do not glisten, spread, be dirty white.
The subtilis SbM-10 that the present invention has nutrition, press down disease and degrading organic phosphor pesticides is a gram-positive microorganism, and the starch hydrolysising experiment is positive, and the V.P. test is positive, and the liquefaction of gelatin puncturing surface is funnel-form.
The present invention has nutrition, abundant, coarse, opaque but the subtilis SbM-10 of disease and degrading organic phosphor pesticides grows, wax, cream color on gravy fine jade inclined-plane.
The present invention has nutrition, press down gemma among the subtilis SbM-10 of disease and degrading organic phosphor pesticides for circular to cylindrical, middle life is given birth to time end, wall is thin, forms in 30hr.
Respectively with Yelkin TTS and Ca
3(PO
4)
2As the organophosphorus source, inorganic phosphorous sources has nutrition to of the present invention, the subtilis SbM-10 that presses down disease and degrading organic phosphor pesticides carries out the mensuration (measuring method is referring to agricultural industry criteria NY412-2000) of phosphorus decomposing performance, measurement result is presented in the inorganic phosphorus fermented liquid, the rapid available phosphorus generation is about 0.45%, in the organophosphorus fermented liquid, the rapid available phosphorus generation is about 1.5%, subtilis SbM-10 of the present invention can not only be with organophosphorus or inorganic phosphorus degraded, also organophosphorus or inorganic phosphorus can be changed into rapid available phosphorus, for crop provides phosphorus plain nutrition, help absorbing of farm crop, the growths of farm crop is had good promoter action; By the degrading organic phosphor pesticides experiment as can be known: subtilis SbM-10 of the present invention (Bacillus subtilis SbM-10) has reached more than 70% the degradation rate of organophosphorus pesticide, compare with existing machine phosphorus insecticide degradation bacteria and other subtilises, the degradation rate height, subtilis of the present invention is effective to organophosphorus pesticide.By bacteriostatic experiment subtilis of the present invention as can be known (Bacillus subtilis) Fusarium oxysporum Fusarium oxysporum, Fusarium sporotrichioides Fusarium sporotrichiodes, Alternariaspp Alternaria, Botrytis cinerea genus Thielaviopsisbasicola, Corynespora Corynespora and colletotrichum Colletotrichum growth all there is the obvious suppression effect.
Subtilis SbM-10 of the present invention produces the bacterial strain from microbial fertilizer, screening obtains, when subtilis of the present invention has nutrition and presses down disease crop, also organophosphorus pesticide had good Degradation, the subtilis SbM-10 that of the present inventionly have nutrition, presses down disease and degrading organic phosphor pesticides has wide range of applications, and extensible microbial fertilizer is in the application in biological restoration field.
Have nutrition, press down the subtilis SbM-10 (Bacillussubtilis SbM-10) of disease and degrading organic phosphor pesticides, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date is on 01 18th, 2010, and preserving number is CGMCC No.3583.
Description of drawings
Fig. 1 is having nutrition, press down the sem photograph of thalline of the subtilis SbM-10 of disease and degrading organic phosphor pesticides in the embodiment one.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: present embodiment has nutrition, presses down the subtilis SbM-10 (Bacillus subtilis SbM-10) of disease and degrading organic phosphor pesticides, in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCC No.3583, and preservation date is on 01 18th, 2010; Subtilis SbM-10 is shaft-like, and length is 2.0～3.0 microns, and wide is 0.7～0.8 micron, catenation, and no pod membrane has gemma; Coarse on beef-protein medium, opaque, do not glisten, spread, be dirty white.
With reference to 2007 by Shen Ping writes, Higher Education Publishing House publishes " microbiology experiment " and 2004 by Yang Wenbo writes, Chemical Industry Press publishes " microbiology experiment " to present embodiment have nutrition, but the subtilis SbM-10 of disease and degrading organic phosphor pesticides carries out the physio-biochemical characteristics evaluation: subtilis is a gram-positive microorganism, the starch hydrolysising experiment is positive, V.P. test positively, gelatin puncturing surface liquefaction is funnel-form.
The optimum growth temperature that has nutrition, presses down the subtilis SbM-10 of disease and degrading organic phosphor pesticides of present embodiment is 28～30 ℃.
Present embodiment has nutrition, the screening method that presses down the subtilis SbM-10 of disease and degrading organic phosphor pesticides carries out in accordance with the following methods: picking 1～2 ring from the microbial fertilizer bacterial strain inclined-plane that activates, be inoculated in and contain in the minimal medium that mass concentration is 40% Rogor, in temperature is 30 ℃, rotating speed is shaking culture 48～72hr under the condition of 150r/min, increase the mass ratio of Rogor in the liquid minimal medium gradually, the mass ratio that Rogor accounts for minimal medium is followed successively by 4 ‰, 5 ‰, 7 ‰, 8 ‰, when cultivating, observe the muddy degree of each thalli growth situation and fermented liquid, choose fast growth, the bacterial strain of better tolerance, by mensuration, finally determine to have nutrition to Rogor residual quantity in the fermented liquid, the subtilis Bacillus subtilis that presses down disease and degrading organic phosphor pesticides; Wherein minimal medium is by 0.2gNaCl, 0.5g MgSO
4, 0.5g K
2SO
4, 0.2gCaCO
3, 0.001g FeSO
4And 1000mlH
2O forms.
Present embodiment have nutrition, press down disease and degrading organic phosphor pesticides subtilis SbM-10 thalline sem photograph as shown in Figure 1.
The subtilis SbM-10 that has nutrition, presses down disease and degrading organic phosphor pesticides of present embodiment is carried out the experiment of phosphorus decomposing effect and presses down the effect experiment of sick effect experiment, and concrete grammar is as follows:
One, phosphorus decomposing effect experiment
1, phosphorus decomposing effect experiment (trophism): respectively with Yelkin TTS as organophosphorus source, Ca
3(PO
4)
2Be inorganic phosphorous sources, carry out the mensuration (measuring method is referring to agricultural industry criteria NY412-2000) of bacterial strain phosphorus decomposing performance, subtilis (present embodiment have nutrition, press down the subtilis SbM-10 of disease and degrading organic phosphor pesticides) is inoculated in respectively in organophosphorus or the inorganic phosphorus liquid nutrient medium with 0.5% inoculum size, is that 30 ℃, rotating speed are shaking culture 72hr under the condition of 180r/min in temperature; Get fermented liquid 10ml and add an amount of without phosphorus gac 1g decolouring, 12000r/min low-temperature centrifugation 20min, get the 5ml supernatant liquor, adopt molybdenum antimony scandium colorimetry, Lambda 20 spectrophotometer 680nm carry out the mensuration of available phosphorus contents, measurement result is in the inorganic phosphorus fermented liquid, and available phosphorus contents is 0.432%; In the organophosphorus fermented liquid, available phosphorus contents is 1.481%; The subtilis SbM-10 of present embodiment can not only also can change into rapid available phosphorus with organophosphorus or inorganic phosphorus with the degraded of organophosphorus or inorganic phosphorus, for crop provides phosphorus plain nutrition, helps the absorption of farm crop, and the growth of farming is had good promoter action; The subtilis of present embodiment has tangible trophism to crop.
2, degrading organic phosphor pesticides Rogor
Adopt same experimental technique, carry out the experiment of degrading organic phosphor Rogor effect.
First group of experiment: test with inorganic salt liquid substratum and meat soup liquid nutrient medium respectively; Adding mass concentration by 2 ‰ mass percents in inorganic salt liquid substratum or meat soup liquid nutrient medium is 40% Rogor (sand swell reaches Zhengzhou agricultural chemicals company limited and produces), and wherein inorganic salt liquid is cultivated by 0.5g KH
2PO
4, 0.5g K
2HPO
4, 0.2MgSO
4, 0.1gCaCl
2, 0.2gNaCl, 0.0001gMnSO
4, 0.0001gFeCl
3, 1.0g (NH
4)
2SO
4And 1000mlH
2O forms, and the meat soup liquid nutrient medium is by 3.0g extractum carnis, 10.0g peptone, 5.0g NaCl and 1000ml H
2O forms.
Second group of experiment: test with inorganic salt liquid substratum and meat soup liquid nutrient medium respectively; Adding mass concentration by 4 ‰ mass percents in inorganic salt liquid substratum or meat soup liquid nutrient medium is 40% Rogor (sand swell reaches Zhengzhou agricultural chemicals company limited and produces), and wherein inorganic salt liquid is cultivated by 0.5g KH
2PO
4, 0.5g K
2HPO
4, 0.2MgSO
4, 0.1gCaCl
2, 0.2gNaCl, 0.0001gMnSO
4, 0.0001gFeCl
3, 1.0g (NH
4)
2SO
4And 1000mlH
2O forms, and the meat soup liquid nutrient medium is by 3.0g extractum carnis, 10.0g peptone, 5.0g NaCl and 1000ml H
2O forms.
The concrete operations of these two groups experiments are as follows: after substratum adds Rogor, the inoculum size of subtilis SbM-10 (Bacillus subtilis SbM-10) according to 1% of present embodiment inserted in the above-mentioned substratum, is that 30 ℃, rotating speed are shaking culture 72hr under the condition of 150r/min in temperature; Get fermented liquid 10ml then, add 20ml acetonitrile (gas-chromatography level), 2gNaCl fully shakes, static 30min draws the 10ml organic phase, and it is dried that 80 ℃ of water-baths are evaporated to, and adds 10ml acetone (gas-chromatography level) dissolving, is used for gas Chromatographic Determination.
Chromatographic condition: chromatographic instrument Agilent 6890N; Automatic sampler Agilent 768; Chromatographic column 50% polyphenyl methyl siloxane (DB-17), 30m * 0.53mm * 1.0 μ m; Injector temperature: 220 ℃; Detector temperature: 250 ℃; Column temperature: 150 ℃ (keeping 2min) → 250 ℃ (keeping 12min), 8 ℃/min of heat-up rate.Gas and flow carrier gas: nitrogen, purity 〉=99.999%, flow velocity are 10mL/min.Combustion gas: hydrogen, purity 〉=99.999%, flow velocity are 75mL/min; Combustion-supporting gas: air, flow velocity are 100mL/min.The degradation rate measuring method of Rogor is with reference to Chinese agriculture industry standard NY/T 761-2008.The measurement result of these two groups experiments is as shown in table 2.
Table 2 subtilis is to the degradation rate of Rogor
Data from table 2 as can be seen, first group is adopted experimentizing of inorganic salt liquid substratum, is 79.79% to the degradation rate of Rogor, adopts experimentizing of meat soup liquid nutrient medium, is 91.49% to the degradation rate of Rogor; Second group is adopted experimentizing of inorganic salt liquid substratum, is 73.59% to the degradation rate of Rogor, adopts experimentizing of meat soup liquid nutrient medium, is 84.97% to the degradation rate of Rogor.
By these two groups experiments as can be seen, for the Rogor of different concns, the subtilis of present embodiment all has good degradation effect.
Two, press down sick effect experiment
Press down sick effect: under the laboratory condition, picking has activated bacterial strain (present embodiment have nutrition, press down the subtilis SbM-10 of disease and degrading organic phosphor pesticides) inclined-plane 1～2 and has encircled in sterilized water, vibration evenly, draw the 0.05ml bacteria suspension on the PDA culture medium flat plate, after smoothening with glass slicker, inoculate each pathogenic bacteria respectively at dull and stereotyped center, simultaneously, pathogenic bacteria is inoculated on the blank flat board in contrast the about 5mm of bacterium piece diameter.Each handles 3 repetitions, 30 ℃ of constant temperature culture, and 5～7d observes upgrowth situation, measures each dull and stereotyped pathogenic bacteria colony diameter of going up, and calculates bacteriostasis rate:
(the treatment group pathogenic bacteria colony diameter of A-inoculation bacterium, cm, B-control group pathogenic bacteria colony diameter, cm, 0.5-inoculation point pathogenic bacteria colony diameter, cm) measurement result shows: subtilis (Bacillus subtilis), to Fusarium oxysporum Fusarium oxysporum, Fusarium sporotrichioides Fusarium sporotrichiodes, Alternariaspp Alternaria, Botrytis cinerea belongs to Thielaviopsisbasicola, Corynespora Corynespora and colletotrichum Colletotrichum growth all has the obvious suppression effect, is 44.2% to the inhibiting rate of Fusarium oxysporum Fusarium oxysporum, inhibiting rate to Fusarium sporotrichioides Fusarium sporotrichiodes is 81.0%, inhibiting rate to Alternariaspp Alternaria is 45.2%, the inhibiting rate that Botrytis cinerea is belonged to Thielaviopsis basicola is 52.1%, inhibiting rate to Corynespora Corynespora is 47.1%, inhibiting rate to colletotrichum Colletotrichum is 100%.The subtilis of present embodiment is as shown in table 1 to the influence of pathogenic bacteria growth.
Table 1 subtilis is to the influence of pathogenic bacteria growth
Annotate: CK represents not inoculate the control group pathogenic bacteria colony diameter of subtilis, handles expression inoculation subtilis pathogenic bacteria colony diameter.
Claims (2)
1. subtilis that has nutrition, presses down disease and degrading organic phosphor pesticides, it is characterized in that having nutrition, press down the subtilis SbM-10 (Bacillus subtilisSbM-10) of disease and degrading organic phosphor pesticides, in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCC No.3583, and preservation date is on 01 18th, 2010; Subtilis SbM-10 is shaft-like, and length is 2.0～3.0 microns, and wide is 0.7～0.8 micron, catenation, and no pod membrane has gemma; Coarse on beef-protein medium, opaque, do not glisten, spread, be dirty white.
2. a kind of subtilis that has nutrition, presses down disease and degrading organic phosphor pesticides according to claim 1, the subtilis SbM-10 that it is characterized in that having nutrition, presses down disease and degrading organic phosphor pesticides is a gram-positive microorganism, the starch hydrolysising experiment is positive, V.P. test positively, gelatin puncturing surface liquefaction is funnel-form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101093433A CN101787354B (en) | 2010-02-11 | 2010-02-11 | Nutritious bacillus subtilis capable of inhibiting diseases and degrading organic phosphorus pesticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101093433A CN101787354B (en) | 2010-02-11 | 2010-02-11 | Nutritious bacillus subtilis capable of inhibiting diseases and degrading organic phosphorus pesticide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101787354A true CN101787354A (en) | 2010-07-28 |
| CN101787354B CN101787354B (en) | 2012-06-06 |
Family
ID=42530734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101093433A Expired - Fee Related CN101787354B (en) | 2010-02-11 | 2010-02-11 | Nutritious bacillus subtilis capable of inhibiting diseases and degrading organic phosphorus pesticide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101787354B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061273A (en) * | 2010-11-05 | 2011-05-18 | 南开大学 | Bacillus subtilis with phosphate-solubilizing effect and application thereof |
| CN102533590A (en) * | 2011-12-14 | 2012-07-04 | 建设部水处理新技术产业化基地(天津港保税区水处理新技术产业化基地) | Bacillus subtilis for removing phosphorus in sewage at low temperatures and isolation and culture method |
| CN102876608A (en) * | 2012-09-26 | 2013-01-16 | 中国医药研究开发中心有限公司 | Bacillus amyloliquefaciens and application thereof |
| CN104087544A (en) * | 2014-06-19 | 2014-10-08 | 徐州工程学院 | Engineering bacterium capable of degrading organophosphorus pesticides, and construction method and application thereof |
| CN105176885A (en) * | 2015-10-19 | 2015-12-23 | 山东宝来利来生物工程股份有限公司 | Bacillus subtilis and application of bacillus subtilis in degrading organophosphorus pesticide |
| CN114196601A (en) * | 2022-01-14 | 2022-03-18 | 甘肃省科学院生物研究所 | Microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticide and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101024819A (en) * | 2006-02-20 | 2007-08-29 | 中国科学院动物研究所 | DU-E4LP seudomonas putida and use in degradating parathionmethyl |
-
2010
- 2010-02-11 CN CN2010101093433A patent/CN101787354B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101024819A (en) * | 2006-02-20 | 2007-08-29 | 中国科学院动物研究所 | DU-E4LP seudomonas putida and use in degradating parathionmethyl |
Non-Patent Citations (2)
| Title |
|---|
| 《中国农学通报》 20080731 马丽娜，等 乐果降解菌的分离、筛选和鉴定 441－444 1－2 第24卷, 第7期 2 * |
| 《四川师范大学学报(自然科学版)》 20010731 杨小蓉，等 一株降解氧乐果的高效菌的分离和鉴定 392－394 1－2 第24卷, 第4期 2 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061273A (en) * | 2010-11-05 | 2011-05-18 | 南开大学 | Bacillus subtilis with phosphate-solubilizing effect and application thereof |
| WO2012058835A1 (en) * | 2010-11-05 | 2012-05-10 | 南开大学 | Bacillus subtilis strain with phosphorus-decomposing ability and application thereof |
| CN102061273B (en) * | 2010-11-05 | 2012-09-12 | 南开大学 | Bacillus subtilis with phosphate-solubilizing effect and application thereof |
| CN102533590A (en) * | 2011-12-14 | 2012-07-04 | 建设部水处理新技术产业化基地(天津港保税区水处理新技术产业化基地) | Bacillus subtilis for removing phosphorus in sewage at low temperatures and isolation and culture method |
| CN102876608A (en) * | 2012-09-26 | 2013-01-16 | 中国医药研究开发中心有限公司 | Bacillus amyloliquefaciens and application thereof |
| CN102876608B (en) * | 2012-09-26 | 2014-01-29 | 中国医药研究开发中心有限公司 | Bacillus amyloliquefaciens and application thereof |
| CN104087544A (en) * | 2014-06-19 | 2014-10-08 | 徐州工程学院 | Engineering bacterium capable of degrading organophosphorus pesticides, and construction method and application thereof |
| CN105176885A (en) * | 2015-10-19 | 2015-12-23 | 山东宝来利来生物工程股份有限公司 | Bacillus subtilis and application of bacillus subtilis in degrading organophosphorus pesticide |
| CN114196601A (en) * | 2022-01-14 | 2022-03-18 | 甘肃省科学院生物研究所 | Microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticide and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101787354B (en) | 2012-06-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lehmann et al. | Biochar effects on soil biota–a review | |
| CN103571771B (en) | The Screening and Identification of one strain phthalic ester efficient degradation genus bacillus and application thereof | |
| CN101787354B (en) | Nutritious bacillus subtilis capable of inhibiting diseases and degrading organic phosphorus pesticide | |
| CN102250798B (en) | Pseudomonas and microbial agent as well as applications thereof | |
| CN109161506A (en) | One bacillus subtilis and its application | |
| CN102250794B (en) | Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof | |
| CN101560483B (en) | Lipopeptide-producing bacillus pumilus and application thereof | |
| CN102321554B (en) | Bacillus cereus and its application of being as plant growth-promoting rhizobacteria | |
| CN103555624A (en) | Nitrogen fixation bacillus licheniformis and application thereof | |
| CN106520604B (en) | One plant of nitrogen-fixing bacteria for producing acc deaminase and its application in soil ecology reparation | |
| CN104371954A (en) | Preparation method of Bacillus strain and microbial inoculant thereof | |
| Ray et al. | Brevibacillus | |
| CN103382449B (en) | Streptomyces sp. T2-10 with broad-spectrum insecticidal and antibacterial activity and its application | |
| CN104805033A (en) | Microbacterium sp. J-1 used for degrading plurality of phthalic acid esters | |
| CN102816720B (en) | ACC-deaminase-producing serratia marcescens LJL-11 and application thereof | |
| CN104805036A (en) | Applications of Microbacterium sp. J-1 in degradation of plurality of phthalic acid esters | |
| CN106754497A (en) | A kind of Ke Leishi pneumobacilluses and its microbial inoculum and preparation method and application | |
| CN102260641B (en) | High-efficiency organic pollutant carbendazim degrading bacteria and use thereof | |
| CN102286402B (en) | Soybean rhizosphere azotobacter for producing activated calcium carbonate (ACC) and realizing antagonistic effect on various pathogenic fungi and purpose thereof | |
| CN104312950B (en) | One strain solution ornithine Raoul bacterium and application thereof | |
| CN103451116A (en) | Pseudomonas fluorescens Y13, and preparation method and applications thereof | |
| CN102816721B (en) | ACC-deaminase-producing agrobacterium tumefaciens LJL-6 and application thereof | |
| CN104805018A (en) | Agromyces sp. MT-E used for simultaneous degradation of plurality of phthalic acid esters | |
| CN107354112B (en) | Thioanal degrading bacterium and application thereof | |
| Hozzein et al. | Antimicrobial activities of some alkaliphilic and alkaline-resistant microorganisms isolated from Wadi Araba, the eastern desert of Egypt |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120606 Termination date: 20150211 |
|
| EXPY | Termination of patent right or utility model |