CN101780271A - Medicinal composition of endotoxin and lectin and application thereof - Google Patents

Abstract

The invention relates to a medicinal composition of endotoxin and lectin and application thereof. The medicinal composition is mainly applied to the preparation of medicament for resisting cancer and infection and curing immunologic diseases. The endotoxin and the lectin are a high-efficiency fire-leading infuriating agent and biological tropin of a human immune system, wherein the endotoxin can activate a phagocytic function of a reticuloendothelial system, improve bactericidal action of macrophage and neutrocyte, release endogenous cytotoxic factors and stimulate a host to defense pathogen infection to cause tumor necrosis; the lectin can activate lymphocyte, resist virus and tumor and adjust the immune function of the organism; and the lectin has a synergistic action together with the endotoxin and retards the stress toxicity of the endotoxin so as to break immune tolerance, promote the recovery of bacterial infection, initiate and aggravate diseases, promote the disease inducement, shorten the course of the diseases and improve the therapeutic effect. Animal experiments show that injection containing the endotoxin and the lectin has a tumor inhibition rate of over 68 percent and anti-infection protection rate of over 80 percent and can be applied to resisting infection and tumor and curing immune system diseases.

Description

Pharmaceutical composition of endotoxin and agglutinin and uses thereof

[technical field] the present invention relates to a kind of immunomodulator, is endotoxin and agglutinin Pharmaceutical composition and uses thereof specifically, i.e. endotoxin and the agglutinin Pharmaceutical composition application in the medicine of anticancer infection of preparation human body and treatment disease of immune system.

The social progress of [background technology] mankind nowadays, civilization is flourishing, and the speed that the human immunity systemic-function is evolved lags far behind the progressive speed of society, and people rely on the therapeutical effect of medicine too much, cause a series of illness incidence rate significantly to increase, diseases such as all kinds of infected tumors happen occasionally, the chronic infection of intractable repeated relapsing is as urinary tract infection, oral ulcer, old-slow-bronchial tube, and immunologic function unbalance disease is as scleroderma, lupus erythematosus, psoriasis etc., all be the commonly encountered diseases frequently-occurring disease, still do not have a kind of ideal medicine that can cure so far.The main direction of medicine also is that more bleb is walked around body immune system and directly acted on pathogenic factors for ancient sacrificial utensil generally speaking, cures the symptoms, not the disease often.

Vicious cycle, the slave who makes more people become medicine crosses to spend to take for a long time to medicine and has caused a large amount of deaths.Caught a cold, the doctor says earlier and has a penicillin shot, and is intramuscular injection in the past, is drop now, penicillin is ineffective, cephalosporin has fortunately been arranged, and cephalosporin is ineffective, also has ampicillin, when ampicillin is invalid, can also use cephalosporin instead, when even the drug-fast methicillin-resistant gold of cephalosporin staphylococcus being lost efficacy, people also have last line of defense---vancomycin.But in 1992, the U.S. found vancomycin is produced chemical sproof MRSA first, almost the doctor was forced the available awkward condition of no medicine.

At any time can see the adaptive capacity that antibacterial is surprising in hospital, the intensive antibiotic of effect provides important alternative condition for forming the strong antibacterial of Drug resistance.Along with antibacterial during evolution, defeat a kind of another antibiotic, the arms race become a biological evolution is infected in treatment.This is a constantly circulation of upgrading, can catch the organism of last chance to win victory.Up to now, show that without any sign we will become the victor.Solution is the new strategy based on biological evolution.

There are several states in the human body: immunologic tolerance, the body drug resistance, the Drug resistance pathogenic bacteria, pathogenic bacteria carrier or the like, many difficult miscellaneous diseases and chronic disease promptly are like this, their function of immune system is in certain silence state, be difficult to restrain pathogenic factors, pathogenic factors also is difficult to rapid outburst, in this face-off process, two kinds of results are arranged: " in silence, do not break out, just in silence, be destroyed! " break this silence state by multiple means, impel immune system to play a role, restrain pathogenic factors, this is only right way approach, and we can not get over bleb for ancient sacrificial utensil, replace the human immune system and exercise pharmic function.

After the forties in 20th century because antibiotic and sulfonamides chemicals is evident in efficacy, to the development of this type of medicine and attention degree considerably beyond to the research of biological product.But the Drug resistance of antibacterial is more and more serious, and this makes the Therapist feel great puzzlement, and then seeks efficient immune stimulant.Up to the seventies in last century, French scientist finds that in zoopery levamisole has immune enhancing function, just makes immunotherapy be subjected to general attention, and people recognize the significant role of biological product to control and elimination infectious disease gradually.

At the beginning of the eighties in last century, 0ldham has proposed the theory of biological response modifier, has established the theoretical basis of malignant tumor Biotherapeutics, has just begun the 4th kind of treatment tumor the pattern---biotherapy after operation, radiotherapy, chemotherapy.

At present, the clinical experiment report of diseases such as existing therapeutic vaccine treatment leprosy, leishmaniasis, brucellosis, acquired immune deficiency syndrome (AIDS), chronic viral hepatitis B, but therapeutic effect is very unstable and desirable, also need constantly carry out the optimization of therapeutic dose and immune time, improve vaccine component, the research of renewal additional factor etc.

In numerous treatment vaccines; people more pay attention to man-to-man immunity protection function; and often ignored human inherent, infection antineoplastic instinct function widely; and this function right and wrong are special; all infection and tumor are all produced effect, and are the human self-protection functions that formed from the evolutionary process that protozoacide begins in 1 years.And this nonspecific immunity function, because of evolving with the microorganism struggle and existing, also because of developing with the microorganism struggle.Endotoxin and agglutinin are as mitogen (mitogen), be human immune system's angry agent of kindling and high efficiency and biological opsonin, promote lymphocytic proliferation and differentiation, strengthen the phagocytic function of reticuloendothelial system, wake the geneogenous infection anti-tumor function of human body up, break the immunity of organism tolerance status, the human activin functions of immune system is engulfed the cell (comprising cancerous cell) that kills and wounds elimination and remove xenobiotic and old and feeble ruined change to pathogen comprehensively.And efficiently regulate the physiological function of human body, and keep the stable of organismic internal environment, promote the self-healing ability of human body, thereby reach the purpose of curing the disease.

Endotoxic biologic activity is very complicated, cause immunoreation when low dose of, heavy dose then causes pathological reaction, the pathogenic effects of endotoxin LPS discharges the excessive endogenous cell factor by the activated mononuclear macrophage system and realizes that clinical manifestation has lethal, pyrogen, disseminated inravascular coagulation etc.

Endotoxin enhancing body non-specific immunity and adjuvant effect, a small amount of endotoxin injects body, can produce nonspecific immunity power to a certain degree, promptly stimulates the immunity that produces that multiple microorganism is all had effect by a kind of gram negative bacteria endotoxin.It is enhancing body nonspecific defense function and bring into play anti-infectious function indirectly.

Agglutinin (lectin) is non-immunogenic, can with the single-minded bonded class glycoprotein of saccharide, having the character that makes cell agglutination and polysaccharide precipitation. agglutinin the earliest is to find in plant in 1888, so called after phytohemagglutinin (Phytohaemagglutinin). so far, separated hundreds of kind agglutinin, as ConA ConA, peanut agglatinin PNA, the plain WGA of Fructus Hordei Germinatus, soybean agglutinin SBA.Find that it does not exist only in the plant, also be present in animal and the microorganism, almost see the organism of various lives, be not limited to special organ and tissue.Because its difficult purification, and cost is high, thus all the time only in laboratory as stimulating lymphopoietic reagent.

Agglutinin is a broad-spectrum antiviral drug, can stimulate T lymphopoiesis differentiation to produce a large amount of effector T cells and cytotoxic T cell, and the effector T cell secretion produces a large amount of cytokines (as interferon etc.) and kills and wounds virus, but cytotoxic T cell direct killing virus.Can stimulate the B cell transformation simultaneously is that proliferation and differentiation is a plasma cell behind the plasmablast, and plasma cell produces a large amount of non-specific antibodies virus that neutralizes.Improve the bone marrow hematogenesis function, promote body leukocyte and multinuclear leucocyte quantity obviously to increase; Improve the body cell inducement interferon, enhancing human body immunity power, enhancing antibody forms, and enhancing body is to the phagocytosis of pathogenic microorganism.

Agglutinin and astragalus polysaccharides, levamisole, vidarabine compatibility have obvious synergistic effect; Has summation action with compatibilities such as part antibiotic.About 5-15 minute blood drug level reaches the peak after the agglutinin intramuscular injection, and blood drug level reaches the peak after oral about 1 hour, and metabolite is mainly discharged from urine.

Agglutinin is easy to use, extensive, can use simultaneously with many medicines, has synergism or summation action.In powder, powder, add agglutinin, have the effect of obvious raising existing product curative effect, do not disturb the detection of original product simultaneously, and do not have species specificity, be applicable to various animals.

Agglutinin can use simultaneously with vaccine, can play the effect that remedies the blank phase of immunity, slows down vaccine stress, enhance immunity effect.Agglutinin is broken immunologic tolerance, promotes the recovery of bacterial infection, causes and aggravates disease, and helps it to send out thoroughly, shortens the course of disease, improves curative effect.

Agglutinin is the non-enzyme glycoprotein that is prevalent in the animals and plants.In more than ten years, a large amount of biochemistrys and the basic research of medical science have confirmed the generation of agglutinin and tumor and have shifted closely related in the past.Research to various agglutinin functions and pharmacy characteristic has produced many progress likely, and the agglutinin of individual species has entered clinical trial as anti-tumor medicine.Agglutinin and endotoxin combination provide a new method for resisting AIDS and cancer etc.

[summary of the invention] the purpose of this invention is to provide the Pharmaceutical composition that comprises endotoxin and agglutinin, is used for preparing the application of anticancer infection of human body and treatment disease of immune system medicine as immunomodulator.

First aspect present invention is with two kinds of different lymphocyte activator agent---endotoxin and agglutinin are combined into medicine, and combined effect is in the human immune system.Endotoxin and agglutinin are human immune system's angry agent of kindling and high efficiency and biological opsonin, (mitogen) promotes lymphocytic proliferation and differentiation as mitogen, strengthen the phagocytic function of reticuloendothelial system, wake the geneogenous infection anti-tumor function of human body up, break the immunity of organism tolerance status, the human activin functions of immune system is engulfed the cell (comprising cancerous cell) that kills and wounds elimination and remove xenobiotic and old and feeble ruined change to pathogen comprehensively.And efficiently regulate the physiological function of human body, and keep the stable of organismic internal environment, promote the self-healing ability of human body.

The process inventor studies for a long period of time and finds to contain the composition of medicine of endotoxin and agglutinin, can be widely used in treatment human body infection antitumor and treatment immune system disease.

Endotoxin of the present invention is the gram-negative bacteria cell wall constituent, and chemical constituent is mainly phospholipid-glycocalix, contains endotoxic thalline also at the present invention's row.Endotoxin energy human activin reticuloendothelial system cytophagy improves the bactericidal action of macrophage and neutrophilic leukocyte, and discharges the endogenous cell factor, excites the defence of host to pathogenic infection, and causes neoplasm necrosis.

Agglutinin of the present invention is for discerning and adhere to a class glycoprotein that derives from plant even microorganism or animal of specificity sugar structure, also comprise in the natural compositions such as the animals and plants that contain agglutinin and microorganism or the agglutinin of purification therefrom, the agglutinin of chemosynthesis, the agglutinin or derivatives thereof of modification.Agglutinin energy activated lymphocyte, the antiviral antitumor, and regulate body's immunity; Have synergistic function with endotoxin, slowing down endotoxin stress toxicity; Break immunologic tolerance, promote the recovery of bacterial infection, cause and aggravate disease, help it to send out thoroughly, shorten the course of disease, improve curative effect.

Second aspect present invention be will contain the medicine of endotoxin and agglutinin combination, preparation becomes the injection type that comprises freeze-dried powder, the next day, use once or use once every day, general 10 times is a course of treatment, can increase the course of treatment again according to the state of an illness.

Third aspect present invention provides the various clinical scheme, the described medicine that contains endotoxin and agglutinin combination, by clinical verification, be applicable to that infection comprises the infection of all gram negative bacteria, all fastbacteria and part gram-positive bacterium and part fungus, but and can kill the virus; The antitumor type comprises pulmonary carcinoma, malignant lymphoma, leukemia, breast carcinoma, nasopharyngeal carcinoma, hepatocarcinoma; Treatment immune system disease comprises that psoriasis, lupus erythematosus, diabetes, hyperthyroidism, allergic rhinitis, oral ulcer, rheumatoid arthritis, heavy breathing kick and myasthenia gravis.According to clinical case, this composition of medicine has shown better curative effect.

The medicine that contains endotoxin and agglutinin combination of the present invention, injection enters in the body, perhaps endotoxin is injected separately and entered in the body, cooperate the smelling of fish or mutton stimulating food diet that contains agglutinin again, can play the curative effect of getting twice the result with half the effort, smelling of fish or mutton stimulating food comprises cock, Cyprinus carpio, beef and mutton, seafood, Folium Allii tuberosi, Seem Lablab Album, Fructus Cucurbitae moschatae, Folium Perillae, Rhizoma Zingiberis Recens, medicated beer, sweet wine, multiple smelling of fish or mutton stimulating food alternately or mix and use, also comprise oral from animals and plants the stimulating food composition of purification be agglutinin.

Endotoxin and the agglutinin Pharmaceutical composition treatment clinical disease of containing of the present invention at lesions position or near the lesions position administration, infects the 10 centimetres of administrations in red and swollen position but will depart from, and enriches blood in conjunction with the Chinese medicine QI invigorating, can significantly improve curative effect.The medicine that contains endotoxin and agglutinin can't inject and directly enters blood in the blood vessel, can unite use with other drug; When the patient is in fatigue, hunger, flu and actute infection state, can not use; Red and swollen heating and lump appear in the injection site, are normal immunoreation, belong to normal condition, need not processing meeting Lock-out, and injection for the second time will depart from the redness scleroma position of last injection, not so can cause bigger heating redness even general reaction.

Beneficial effect of the present invention:

1. endotoxin and agglutinin combined effect have complementary potentiation synergism in immune system.

Taylor etc. studies show that: oral fimbriate vibrio cholera attenuation vaccine, antitoxin antibacterial antibody obviously raises in its serum, and clinical symptoms also alleviated manyly, has proved that pilin (agglutinin) is a kind of important protection immunogen.Wild pili thalline vaccine has been successfully used to stop the colibacillary infection of cultivated animals, and pili agglutinin and endotoxin obtain powerful immune protective efficiency by cultivated animals.

2. endotoxin and agglutinin combination, mutual attenuation, increase patient's compliance.

Endogenous toxin have toxicity such as lethal, pyrogen, disseminated inravascular coagulation, and agglutinin can promote the recovery of bacterial infection, increases the weight of sufferer, usually says that clinically " dieting " is exactly this reason.And after both combinations, toxicity subtracts mutually, and as the vibrio cholera attenuation vaccine of Taylor research, the pili agglutinin has been alleviated endotoxic toxicity; The defensive enginery of endotoxin enhancing body is strengthened the removing to pathogen, can offset the saturating toxicity of sending out of agglutinin.

3. endotoxin and agglutinin cooperation, the onset of treatment disease is faster, and shorten the course of treatment, and improve cure rate.

Agglutinin can bring out the obstinate disease chronic disease, adds disease again, breaks tolerance status, and breaking dormancy helps it to send out thoroughly, impels various pathogenic microorganisms to be active state.Endotoxin and agglutinin combined effect are in immune system simultaneously, produce a series of immune response, discharge multiple lymphokine, acting on mononuclear phagocyte system makes it to assemble to lesions position, pathogen is engulfed the cell of eliminating and removing xenobiotic and old and feeble ruined change, reach comprehensively the thoroughly purpose of radical cure.

The present invention is further described below in conjunction with specific embodiment:

[specific embodiment]:

The composite type of embodiment 1. endotoxins and agglutinin has three kinds of modes:

First kind is the combination of gram negative bacteria and pili, promptly contains the whole cell vaccine of pilin.

1. the screening of strain and cultivation: strain should screen expresses the high less-virulent strain of pili, and selects suitable culture medium and cultural method to cultivate, 1% formalin-inactivated thalline, and eccentric cleaning formaldehyde, the cold preservation of collection thalline is standby.

2. the preparation of pilin thalline Pharmaceutical composition: the sterile working, adopt the normal saline preparation of pH7.2 phosphate buffer, above-mentioned cold preservation thalline is diluted to certain density bacteria suspension, add 0.6% benzyl alcohol antiseptic, divide again to be filled in the peace bottle.

Second kind is gram negative bacteria and/or pili, adds the combination of agglutinin.Basic first kind manufacturing process together just adds an amount of agglutinin such as ConA ConA, PHA-P HA, the plain WGA of Fructus Hordei Germinatus etc. in the preparation later stage.

The third is the endotoxin of having purified and the combination of agglutinin.For example in Bacillus typhi endotoxin injection, add an amount of agglutinin, can slow down toxicity, increase curative effect.

The study of pharmacy of the compositions of embodiment 2. endotoxins and agglutinin:

Many coli common pilis have hemagglutinative function, can stimulate bone-marrow-derived lymphocyte to make it differentiation (Ponniah etc., 1989).The pili agglutinin activates bone-marrow-derived lymphocyte can produce polyclone IgM, and reaches summit at the 3rd day.Pili agglutinins such as FinH have the mannose specificity, play a crucial role in activating bone-marrow-derived lymphocyte, and the pili agglutinin also mediates other pathophysiological roles (Ponniah etc., 1992) simultaneously.Endotoxin is present in the somatic cells wall, and the pili whole cell promptly is the compositions of endotoxin and agglutinin, is that example is carried out study of pharmacy at this with the Pseudomonas aeruginosa that contains PilinA mannose specific agglutination element:

1. toxicity test: go into body weight 18-20 gram mice to contain pilin 15,000,000,000 dead thalline lumbar injections, observed 7, none death, vivaciously as usual.Matched group does not contain pilin, same operation, and survival 70%, and 30% feed is not good, and only 40% is vivaciously as usual.

2. immunity and protecting test: select body weight 14-16 gram mice, test group subcutaneous injection 1,000,000,000 pilin thalline, three days are once; inject altogether three times, after 7 days, attack excessively with the toadstool of 3LD50; observed test group survival 80%, matched group (not having subcutaneous injection pilin thalline) 100% death three days.

3. inhibition test: mouse peritoneal inoculation S180 virus, get the mouse ascites oncocyte after 5 days, the groin that oncocyte is inoculated in test mice is subcutaneous, the dead thalline 400,000,000/Mus of treatment group injection pilin, the next day, once inject 8 times altogether, the normal saline of matched group injection equivalent is put to death mice, gets tumor and weighs, calculate tumour inhibiting rate, the tumour inhibiting rate of treatment group as a result can be up to 68%, if also in conjunction with oral agglutinin, effect can be better.

4. the coordinated trials of endotoxin and agglutinin: getting Turnover of Mouse Peritoneal Macrophages stimulates generation IL-1 at the external use endotoxin, stimulates thymocyte cell generation mitosis with the collaborative ConA ConA of IL-1 then, judges the activity of IL-1 with the intensity of division growth.Test itself has just illustrated the Synergistic relation of endotoxin and agglutinin ConA etc., and animal experiment and clinical showing: the Pseudomonas aeruginosa injection can directly transform lymphocyte, and can strengthen the conversion ratio of lymphocyte to ConA ConA.

The clinical practice of the compositions of embodiment 3. endotoxins and agglutinin is an example in this clinical practice with the Pseudomonas aeruginosa injection that contains the pili agglutinin:

1. the curative effect of treatment repeated relapsing urinary tract infection is good, treatment effective percentage 95%, cure rate 92.5%, patient's immunocompromised state obtains obviously to improve, and multiple pathogen is comprised that the urinary tract infection effect due to gram negative bacteria and L type and staphylococcus aureus and the L type thereof is all fine.

2. treat the routine disease of chronic hepatitis B: slowly, the man, 40 years old, people from Hunan, suffer from hepatitis B for many years, taking medicine has an injection has treated several years, fails to fully recover, and the inventor has used ten Pseudomonas aeruginosa injection, and cooperation contains the smelling of fish or mutton stimulating food diet of agglutinin, stimulating food increases the weight of the injection site redness, and immunoreation is strengthened, and the result has cured.

3. treat the case of bone tuberculosis: horse, the woman, 45 years old, people from Hunan, the unknown finger-joint of left hand is suffered from tuberculosis, with antituberculosis drugs treat March, illing skin melanism necrosis is not still felt any better, and the inventor is at her left hand back injection Pseudomonas aeruginosa injection, only used the commentaries on classics of promptly getting better of three pins, the result has used ten medicines to fully recover.

4. treat the case of tumor: once, the woman, 41 years old, people from Hubei, uterus left side attachment tumor, about 4 centimetres of diameter, the left side kidney has four calculus that diameter is about 0.2 centimetre, and right kidney has a microlith.The present patent application people is near position injection Pseudomonas aeruginosa injection tumor directly, the patient is when the 4th pin, eaten the cock stimulating food, cause position pain such as tumor calculus unbearably, remove examination in hospital quickly, the result is: diameter of tumor is 2.4 * 1.8 centimetres, become solid tumor (optimum) by former hollow tumor, calculus also has obviously loosening, continues medication five pins afterwards, and the tumor calculus all disappears.This patient lives the rural area, usually seldom has an injection and takes medicine, and function of immune system is strong.

5. treat the case of allergic asthma: open, the woman, 50 years old, the Pekinese suffered from allergic asthma for many years, fail to respond to any medical treatment, the inventor provides 5 Pseudomonas aeruginosa injection to the patient, after the patient uses two Pseudomonas aeruginosa injection, and the commentaries on classics of promptly getting better, also only use 5 medicines just to fully recover, do not seen recurrence in nearly 2 years.

6. treat psoriasic case: thunder, the man, 35 years old, people from Guangzhou, psoriasis illness symptom moderate, the inventor provides 6 Pseudomonas aeruginosa injection to him, promptly got well a greater part of after using three, originally planning provides medicine to him again, can 6 uses the back disease and has also fully recovered, and does not see recurrence in nearly 1 year.

Claims (8)

1. the purposes of endotoxin and agglutinin Pharmaceutical composition is characterized in that: contain the Pharmaceutical composition of endotoxin and agglutinin, can use in preparation human body infection antitumor and treatment disease of immune system medicine.

2. according to the purposes of described endotoxin of claim 1 and agglutinin Pharmaceutical composition, it is characterized in that: described endotoxin is the gram-negative bacteria cell wall constituent, chemical constituent is mainly phospholipid-glycocalix, contains endotoxic thalline also at the present invention's row.

3. according to the purposes of described endotoxin of claim 1 and agglutinin Pharmaceutical composition, it is characterized in that: described agglutinin is for identification and adhere to the class glycoprotein that derives from plant even microorganism or animal of specificity sugar structure, also comprises in the natural goods of the animals and plants that contain agglutinin and microorganism or the therefrom agglutinin, the agglutinin of chemosynthesis, the agglutinin or derivatives thereof of modification of purification.

4. according to the purposes of described endotoxin of claim 1 and agglutinin Pharmaceutical composition, it is characterized in that: the described medicine that contains endotoxin and agglutinin combination, it is the injection type that comprises freeze-dried powder, the next day, use once or use once every day, general 10 times is a course of treatment, can increase the course of treatment again according to the state of an illness.

5. according to the purposes of described endotoxin of claim 1 and agglutinin Pharmaceutical composition, it is characterized in that: the described medicine that contains endotoxin and agglutinin combination, infection comprises the infection of all gram negative bacteria, all fastbacteria and part gram-positive bacterium and part fungus, but and can kill the virus; The antitumor type comprises pulmonary carcinoma, malignant lymphoma, leukemia, breast carcinoma, nasopharyngeal carcinoma, hepatocarcinoma; Treatment immune system disease comprises psoriasis, lupus erythematosus, diabetes, hyperthyroidism, allergic rhinitis, oral ulcer, rheumatoid arthritis, asthma and myasthenia gravis.

6. according to the purposes of described endotoxin of claim 1 and agglutinin Pharmaceutical composition, it is characterized in that: describedly contain the injection of endotoxin and agglutinin composition of medicine and enter in the body, perhaps endotoxin is injected separately and entered in the body, cooperate the smelling of fish or mutton stimulating food diet that contains agglutinin again, can play the curative effect of getting twice the result with half the effort, smelling of fish or mutton stimulating food comprises cock, Cyprinus carpio, beef and mutton, seafood, Folium Allii tuberosi, Seem Lablab Album, Fructus Cucurbitae moschatae, Folium Perillae, Rhizoma Zingiberis Recens, medicated beer, sweet wine, can be multiple smelling of fish or mutton stimulating food alternately or mix and use, also comprise oral from animals and plants the stimulating food composition of purification be agglutinin.

7. according to the purposes of described endotoxin of claim 1 and agglutinin Pharmaceutical composition, it is characterized in that: described endotoxin and the agglutinin Pharmaceutical composition treatment clinical disease of containing, at lesions position or near the lesions position administration, infect the 10 centimetres of administrations in red and swollen position but depart from, and enrich blood in conjunction with the Chinese medicine QI invigorating, can significantly improve curative effect.

8. according to the purposes of described endotoxin of claim 1 and agglutinin Pharmaceutical composition, it is characterized in that: the described medicine that contains endotoxin and agglutinin, can't inject and directly enter blood in the blood vessel, can unite use with other drug; When the patient is in fatigue, hunger, flu and actute infection state, can not use; Red and swollen heating and lump appear in the injection site, are normal immunoreation, belong to normal condition, need not processing meeting Lock-out, and injection for the second time will depart from the redness scleroma position of last injection, not so can cause bigger heating redness even general reaction.