CN101779733A - Feed additive for improving collagen in livestock and poultry muscle and production method thereof and application thereof - Google Patents
Feed additive for improving collagen in livestock and poultry muscle and production method thereof and application thereof Download PDFInfo
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- CN101779733A CN101779733A CN 201010134182 CN201010134182A CN101779733A CN 101779733 A CN101779733 A CN 101779733A CN 201010134182 CN201010134182 CN 201010134182 CN 201010134182 A CN201010134182 A CN 201010134182A CN 101779733 A CN101779733 A CN 101779733A
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Abstract
The invention relates to the technical field of biological product, in particular to a feed additive for improving collagen in livestock and poultry muscle and a production method thereof and application thereof. The feed additive for improving the collagen in livestock and poultry muscle comprises the active ingredients in percentage by weight: 0.3 to 5 percent of glycine, 0.5 to 7 percent of hydroxylysine, 0.6 to 7 percent of praline, 0.2 to 3 percent of hydroxyproline and 85 to 92 percent of starch (all the contents of the ingredients are calculated at the dry product). The production process is simple, the operation is easy, the product conversion rate is high, the fermentation period is short, no chemical component is added during the production process, and the product is free from toxic and side effect and is environment-friendly, safe and reliable. The feed additive can effectively improve the content of the collagen in the livestock and poultry muscles.
Description
Technical field:
The present invention relates to biological product technical field, particularly a kind of feed addictive and production method and application that improves collagen in the livestock and poultry muscle.
Background technology:
Scientific research shows, is made up of collagen by 70% in the human body skin composition, and collagen makes cell become plentiful, full, keeps skin elasticity with moist, makes skin keep young.Collagen can also reduce triglycerides and the cholesterol in the human body, improves the puzzlement of female diseases, alleviates the various discomforts of climacteric women.In case the shortage collagen can have a strong impact on internal organs health, even cause various diseases, therefore fully replenishing collagen can be assisted the internal organs nutritional supplementation, effectively prevent disease.
Collagen mainly is present in pork skin, beef tendon, chicken wings, cock skin, pig's feet, fish-skin and the cartilage, content seldom in the livestock and poultry meat, but livestock and poultry meat and correlated product are requisite food on the mass consumption dining table, therefore, livestock and poultry meat and the correlated product of developing high collagen content become extremely urgent problem.
Summary of the invention:
The present invention is directed to the existing in prior technology problem, and provide a kind of technical scheme that improves calcium content of livestock and poultry bodies and promoting growth feed addictive and production technology and application, production technology is simple, easy to operate, the conversion rate of products height, fermentation period is short, do not add any chemical composition in the production process, product has no side effect, and environmental protection is safe and reliable.And the described feed addictive of this programme can effectively improve the content of collagen in the livestock and poultry muscle.
The present invention realizes by following technical measures:
A kind of feed addictive that improves collagen in the livestock and poultry muscle, it is characterized in that the active ingredient and the percentage by weight thereof that comprise are: glycine 0.3-5%, oxylysine 0.5-7%, proline 0.6-7%, hydroxyproline 0.2-3%, starch 85-92% (above content calculates with dry product).
The production technology of the feed addictive of collagen in the raising livestock and poultry muscle of the present invention may further comprise the steps:
(1), fermentation tank, seeding tank, slack tank sterilization and pipeline sterilization:
First water flushing pipe and fermentation tank before producing, rinse well, open each turnover valve of each pipeline and fermentation tank, seeding tank except that waterpipe then, feed the steam of 0.13-0.14 MPa then, allow steam and each pipeline valve UNICOM, discharged steam 20-25 minute, close each drain tap then, allow the steam pressure of system the inside remain on the 0.13-0.14 MPa, keep just can reaching in 50-55 minute the effect of thorough sterilization, it is standby by cooling to 28 ℃-30 ℃ inside the system naturally to close the steam main valve then.
(2), the rhodotorula mucilaginosa bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 10%, dipotassium hydrogen phosphate 0.25%, yeast extract 0.25%, raw soya bean powder 0.5%, water 89%;
More than mix and dissolve, pH value is 6-6.5;
Concrete operations:
Get 500 milliliters of triangular flasks, 150 milliliters of every bottled above nutrient solutions, the sealing of triangular flask bottleneck, putting into medical disinfecting sterilizes, connect power supply, when sterilizer inner vapor pressure reaches 0.05 MPa, open the cold air in the air bleeding valve discharge sterilizer on the sterilizer, when Pressure gauge points to zero, close air bleeding valve, continue heating, when sterilizer inner vapor pressure reaches the 0.14-0.15 MPa, keep can reaching in 35-40 minute the effect of thorough sterilization, stop heating, allow it lower the temperature naturally, when the Pressure gauge of sterilizer points to zero, open air bleeding valve, open the triangular flask that sterilizer takes out the bacterium of having gone out again, be put into and cool to 28 ℃-30 ℃ on the superclean bench naturally, under the germ-free condition, each triangular flask inserts the Rhodotorula mucilaginose 0.1-0.2 gram of buying, and seals bottleneck then, be put in the biochemical incubator of electric heating, got final product in 36-40 hour cultivating under the condition of 28 ℃-30 ℃ of temperature (more than be called the triangular flask liquid strain);
(3) the seeding tank bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.5%, yeast extract 2%, raw soya bean powder 3%, water 91.5%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the seeding tank of the bacterium of having gone out, start mixer, add cerelose more successively, dipotassium hydrogen phosphate, yeast extract, the raw soya bean powder, add the back stirring fully dissolved in 30-35 minute, mixer Revolution Per Minute 150 changes, close charging aperture then, the feeding steam is heated, when steam pressure in the seeding tank reaches the 0.14-0.15 MPa, keep just can reaching in 55-60 minute the effect of thorough sterilization, feed cooling water circulation then, when the culture medium in the seeding tank drops to 28 ℃-30 ℃, under aseptic condition, open the inoculation cap, insert the triangular flask liquid strain in seeding tank, each hundred kilograms of seed tank culture liquid inserts triangular flask liquid strain 1500-2000 milliliter, build the inoculation cap then, the beginning seed culture;
Condition of culture: the pH value nature, 28 ℃-30 ℃ of temperature, mixer Revolution Per Minute 120-140 changes, incubation time 30-35 hour;
(4) fermentation tank culture:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.2%, yeast extract 1%, raw soya bean powder 5%, corn flour 4%, wheat bran skin 8%, water 78.8%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the fermentation tank of slack tank sterilization, start mixer, Revolution Per Minute 150-180 changes, add the raw soya bean powder more successively, corn flour, yeast extract, the wheat bran skin, glucose, dipotassium hydrogen phosphate, add back continuation stirring and made it even in 30 minutes, the steam that feeds the 0.14-0.15 MPa then begins to heat, when steam pressure in the fermentation tank reaches the 0.14-0.15 MPa, keep just can reaching in 55-60 minute the effect of thorough sterilization, close steam then, feed the running water cooling by the jar interlayer, when temperature in the jar drops to 28 ℃-30 ℃, begin to connect the cultured Rhodotorula mucilaginose of seeding tank, at first improve seeding tank pressure when 0.1 MPa, fermentation tank tank pressure 0.05 MPa, open the inoculation pipeline valve, the seeding tank seed is transported in the fermentation tank goes, the seed consumption is between the 3%-5%;
Fermentation condition: pH value nature, 28 ℃-30 ℃ of temperature, mixer Revolution Per Minute 200-220 changes, cultivate and began to measure glycine content in 30 hours, measured once every two hours later on, when glycine content reaches 2.8%-3% in the zymotic fluid, (calculate) and just reach fermentation termination, can reach fermentation and finishing in general 40-45 hour with dry product;
(5) finished product extracts:
Zymotic fluid is warmed to 50 ℃-55 ℃, centrifugal with the yeast centrifuge, discard clear liquid and collect wet yeast, between 50 ℃-60 ℃, use the air blast drying machine drying, when moisture between 10%-12%, ultramicro grinding is finished product.
Consumption and the effect of this feed addictive in animal and fowl fodder is:
In livestock feed, add 0.5%-0.8%; In bird feed, add 0.6%-1%, can reach the effect that improves collagen in the livestock and poultry muscle.
Object lesson is as follows:
1, in pig feed, adds additive 0.5%-0.8% of the present invention by weight percentage, according to normal breed, can improve collagen content in the pork to 3%-4.8%, contain in the common pork between the about 0.5%-0.6% of collagen, compare with common pork and to have improved 6-8 doubly, do not influence growth (testing with pig variety is the Shanghai White pig) in the breeding process.
2, in the chicken feed by weight percentage add additive 0.6%-1% of the present invention, according to normal breed, can improve collagen content in the chicken to 0.75%-1.2%, contain in the common chicken between the about 0.15%-0.2% of collagen, compare with common chicken and to have improved 5-6 doubly, do not influence growth (testing with chicken breed is hypo sieve fryer) in the breeding process.
This feed addictive is a solid, powdery, directly adds to by consumption and mixes thoroughly in the feed and can feed.
Beneficial effect of the present invention can be learnt by foregoing description, this programme production technology is simple, easy to operate, the conversion rate of products height, fermentation period is short, do not add any chemical composition in the production process, employed bacterial classification is the food-grade barms that uses in the world, and properties of product are stablized, had no side effect, environmental protection, safe and reliable, can effectively improve the content of collagen in the livestock and poultry muscle.There are not waste water, toxic emission in the production.
The specific embodiment:
For further specifying the present invention, below specifically set forth by several embodiment.
Embodiment 1
Main production equipments:
Bacterial classification culture device: the biochemical incubator of electric heating, superclean bench, medical sterilization pot.
Zymolysis Equipment: stirred-tank fermenter, seeding tank.
Preliminary treatment and finished product equipment: yeast centrifuge, blast drier, micronizer.
Production technology:
(1), fermentation tank, seeding tank, slack tank sterilization and pipeline sterilization:
First water flushing pipe and fermentation tank before producing, rinse well, open each turnover valve of each pipeline and fermentation tank, seeding tank except that waterpipe then, feed the steam of 0.13 MPa then, allow steam and each pipeline valve UNICOM, discharged steam 20 minutes, close each drain tap then, allow the steam pressure of system the inside remain on 0.13 MPa, keep just can reaching in 50 minutes the effect of thorough sterilization, it is standby by cooling to 28 ℃ inside the system naturally to close the steam main valve then.
(2), the rhodotorula mucilaginosa bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 10%, dipotassium hydrogen phosphate 0.25%, yeast extract 0.25%, raw soya bean powder 0.5%, water 89%;
More than mix and dissolve, pH value is 6-6.5;
Concrete operations:
Get 500 milliliters of triangular flasks, 150 milliliters of every bottled above nutrient solutions, the sealing of triangular flask bottleneck, putting into medical disinfecting sterilizes, connect power supply, when sterilizer inner vapor pressure reaches 0.05 MPa, open the cold air in the air bleeding valve discharge sterilizer on the sterilizer, when Pressure gauge points to zero, close air bleeding valve, continue heating, when sterilizer inner vapor pressure reaches 0.14 MPa, keep can reaching in 35 minutes the effect of thorough sterilization, stop heating, allow it lower the temperature naturally, when the Pressure gauge of sterilizer points to zero, open air bleeding valve, open the triangular flask that sterilizer takes out the bacterium of having gone out again, be put into and cool to 28 ℃ on the superclean bench naturally, under the germ-free condition, each triangular flask inserts Rhodotorula mucilaginose 0.1 gram of buying, and seals bottleneck then, be put in the biochemical incubator of electric heating, got final product in 36 hours cultivating under the condition of 28 ℃ of temperature (more than be called the triangular flask liquid strain);
(3) the seeding tank bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.5%, yeast extract 2%, raw soya bean powder 3%, water 91.5%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the seeding tank of the bacterium of having gone out, start mixer, add cerelose more successively, dipotassium hydrogen phosphate, yeast extract, the raw soya bean powder, add the back stirring fully dissolved in 30 minutes, mixer Revolution Per Minute 150 changes, close charging aperture then, the feeding steam is heated, when steam pressure in the seeding tank reaches 0.14 MPa, keep just can reaching in 55 minutes the effect of thorough sterilization, feed cooling water circulation then, when the culture medium in the seeding tank drops to 28 ℃, under aseptic condition, open the inoculation cap, insert the triangular flask liquid strain in seeding tank, each hundred kilograms of seed tank culture liquid inserts 1500 milliliters of triangular flask liquid strains, build the inoculation cap then, the beginning seed culture;
Condition of culture: the pH value nature, 28 ℃ of temperature, mixer Revolution Per Minute 120 changes incubation time 30 hours;
(4) fermentation tank culture:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.2%, yeast extract 1%, raw soya bean powder 5%, corn flour 4%, wheat bran skin 8%, water 78.8%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the fermentation tank of slack tank sterilization, start mixer, Revolution Per Minute 150 changes, add the raw soya bean powder more successively, corn flour, yeast extract, the wheat bran skin, glucose, dipotassium hydrogen phosphate, add back continuation stirring and made it even in 30 minutes, the steam that feeds 0.14 MPa then begins to heat, when steam pressure in the fermentation tank reaches 0.14 MPa, keep just can reaching in 55 minutes the effect of thorough sterilization, close steam then, feed the running water cooling by the jar interlayer, when temperature in the jar drops to 28 ℃, begin to connect the cultured Rhodotorula mucilaginose of seeding tank, at first improve seeding tank pressure when 0.1 MPa, fermentation tank tank pressure 0.05 MPa, open the inoculation pipeline valve, the seeding tank seed is transported in the fermentation tank goes, the seed consumption is 3%;
Fermentation condition: pH value nature, 28 ℃ of temperature, mixer Revolution Per Minute 200 changes, cultivate and began to measure glycine content in 30 hours, measured once every two hours later on, when glycine content reaches 2.8%-3% in the zymotic fluid, (calculate) and just reach fermentation termination, can reach fermentation and finish in 40 hours with dry product;
(5) finished product extracts:
Zymotic fluid is warmed to 50 ℃, centrifugal with the yeast centrifuge, discard clear liquid and collect wet yeast, use the air blast drying machine drying at 50 ℃, when moisture 10% the time, ultramicro grinding is finished product.
Consumption and the effect of this feed addictive in animal and fowl fodder is:
In pig feed, add additive 0.5%-0.8% of the present invention by weight percentage, according to normal breed, can improve collagen content in the pork to 3%-4.8%, contain in the common pork between the about 0.5%-0.6% of collagen, compare with common pork and to have improved 6-8 doubly, do not influence growth (testing with pig variety is the Shanghai White pig) in the breeding process.
This feed addictive is a solid, powdery, directly adds to by consumption and mixes thoroughly in the feed and can feed.
Embodiment 2
Main production equipments:
Bacterial classification culture device: the biochemical incubator of electric heating, superclean bench, medical sterilization pot.
Zymolysis Equipment: stirred-tank fermenter, seeding tank.
Preliminary treatment and finished product equipment: yeast centrifuge, blast drier, micronizer.
Production technology:
(1), fermentation tank, seeding tank, slack tank sterilization and pipeline sterilization:
First water flushing pipe and fermentation tank before producing, rinse well, open each turnover valve of each pipeline and fermentation tank, seeding tank except that waterpipe then, feed the steam of 0.14 MPa then, allow steam and each pipeline valve UNICOM, discharged steam 25 minutes, close each drain tap then, allow the steam pressure of system the inside remain on 0.14 MPa, keep just can reaching in 55 minutes the effect of thorough sterilization, it is standby by cooling to 30 ℃ inside the system naturally to close the steam main valve then.
(2), the rhodotorula mucilaginosa bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 10%, dipotassium hydrogen phosphate 0.25%, yeast extract 0.25%, raw soya bean powder 0.5%, water 89%;
More than mix and dissolve, pH value is 6-6.5;
Concrete operations:
Get 500 milliliters of triangular flasks, 150 milliliters of every bottled above nutrient solutions, the sealing of triangular flask bottleneck, putting into medical disinfecting sterilizes, connect power supply, when sterilizer inner vapor pressure reaches 0.05 MPa, open the cold air in the air bleeding valve discharge sterilizer on the sterilizer, when Pressure gauge points to zero, close air bleeding valve, continue heating, when sterilizer inner vapor pressure reaches 0.15 MPa, keep can reaching in 40 minutes the effect of thorough sterilization, stop heating, allow it lower the temperature naturally, when the Pressure gauge of sterilizer points to zero, open air bleeding valve, open the triangular flask that sterilizer takes out the bacterium of having gone out again, be put into and cool to 30 ℃ on the superclean bench naturally, under the germ-free condition, each triangular flask inserts Rhodotorula mucilaginose 0.2 gram of buying, and seals bottleneck then, be put in the biochemical incubator of electric heating, got final product in 40 hours cultivating under the condition of 30 ℃ of temperature (more than be called the triangular flask liquid strain);
(3) the seeding tank bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.5%, yeast extract 2%, raw soya bean powder 3%, water 91.5%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the seeding tank of the bacterium of having gone out, start mixer, add cerelose more successively, dipotassium hydrogen phosphate, yeast extract, the raw soya bean powder, add the back stirring fully dissolved in 35 minutes, mixer Revolution Per Minute 150 changes, close charging aperture then, the feeding steam is heated, when steam pressure in the seeding tank reaches 0.15 MPa, keep just can reaching in 60 minutes the effect of thorough sterilization, feed cooling water circulation then, when the culture medium in the seeding tank drops to 30 ℃, under aseptic condition, open the inoculation cap, insert the triangular flask liquid strain in seeding tank, each hundred kilograms of seed tank culture liquid inserts 2000 milliliters of triangular flask liquid strains, build the inoculation cap then, the beginning seed culture;
Condition of culture: the pH value nature, 30 ℃ of temperature, mixer Revolution Per Minute 140 changes incubation time 35 hours;
(4) fermentation tank culture:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.2%, yeast extract 1%, raw soya bean powder 5%, corn flour 4%, wheat bran skin 8%, water 78.8%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the fermentation tank of slack tank sterilization, start mixer, Revolution Per Minute 180 changes, add the raw soya bean powder more successively, corn flour, yeast extract, the wheat bran skin, glucose, dipotassium hydrogen phosphate, add back continuation stirring and made it even in 30 minutes, the steam that feeds 0.15 MPa then begins to heat, when steam pressure in the fermentation tank reaches 0.15 MPa, keep just can reaching in 60 minutes the effect of thorough sterilization, close steam then, feed the running water cooling by the jar interlayer, when temperature in the jar drops to 30 ℃, begin to connect the cultured Rhodotorula mucilaginose of seeding tank, at first improve seeding tank pressure when 0.1 MPa, fermentation tank tank pressure 0.05 MPa, open the inoculation pipeline valve, the seeding tank seed is transported in the fermentation tank goes, the seed consumption is 5%;
Fermentation condition: pH value nature, 30 ℃ of temperature, mixer Revolution Per Minute 220 changes, cultivate and began to measure glycine content in 30 hours, measured once every two hours later on, when glycine content reaches 2.8%-3% in the zymotic fluid, (calculate) and just reach fermentation termination, can reach fermentation and finish in 45 hours with dry product;
(5) finished product extracts:
Zymotic fluid is heated 55 ℃, centrifugal with the yeast centrifuge, discard clear liquid and collect wet yeast, in the time of 50 ℃, use the air blast drying machine drying, when moisture 12% the time, ultramicro grinding is finished product.
Consumption and the effect of this feed addictive in animal and fowl fodder is:
Percentage adds additive 0.6%-1% of the present invention by weight in the chicken feed, according to normal breed, can improve collagen content in the chicken to 0.75%-1.2%, contain in the common chicken between the about 0.15%-0.2% of collagen, compare with common chicken and to have improved 5-6 doubly, do not influence growth (testing with chicken breed is hypo sieve fryer) in the breeding process.
This feed addictive is a solid, powdery, directly adds to by consumption and mixes thoroughly in the feed and can feed.
Claims (3)
1. feed addictive that improves collagen in the livestock and poultry muscle, it is characterized in that the active ingredient and the percentage by weight thereof that comprise are: glycine 0.3-5%, oxylysine 0.5-7%, proline 0.6-7%, hydroxyproline 0.2-3%, starch 85-92% (above content calculates with dry product).
2. the production method of the feed addictive of collagen in the raising livestock and poultry muscle as claimed in claim 1 is characterized in that may further comprise the steps:
(1), fermentation tank, seeding tank, slack tank sterilization and pipeline sterilization:
First water flushing pipe and fermentation tank before producing, rinse well, open each turnover valve of each pipeline and fermentation tank, seeding tank except that waterpipe then, feed the steam of 0.13-0.14 MPa then, allow steam and each pipeline valve UNICOM, discharged steam 20-25 minute, close each drain tap then, allow the steam pressure of system the inside remain on the 0.13-0.14 MPa, keep just can reaching in 50-55 minute the effect of thorough sterilization, it is standby by cooling to 28 ℃-30 ℃ inside the system naturally to close the steam main valve then.
(2), the rhodotorula mucilaginosa bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 10%, dipotassium hydrogen phosphate 0.25%, yeast extract 0.25%, raw soya bean powder 0.5%, water 89%;
More than mix and dissolve, pH value is 6-6.5;
Concrete operations:
Get 500 milliliters of triangular flasks, 150 milliliters of every bottled above nutrient solutions, the sealing of triangular flask bottleneck, putting into medical disinfecting sterilizes, connect power supply, when sterilizer inner vapor pressure reaches 0.05 MPa, open the cold air in the air bleeding valve discharge sterilizer on the sterilizer, when Pressure gauge points to zero, close air bleeding valve, continue heating, when sterilizer inner vapor pressure reaches the 0.14-0.15 MPa, keep can reaching in 35-40 minute the effect of thorough sterilization, stop heating, allow it lower the temperature naturally, when the Pressure gauge of sterilizer points to zero, open air bleeding valve, open the triangular flask that sterilizer takes out the bacterium of having gone out again, be put into and cool to 28 ℃-30 ℃ on the superclean bench naturally, under the germ-free condition, each triangular flask inserts the Rhodotorula mucilaginose 0.1-0.2 gram of buying, and seals bottleneck then, be put in the biochemical incubator of electric heating, got final product in 36-40 hour cultivating under the condition of 28 ℃-30 ℃ of temperature (more than be called the triangular flask liquid strain);
(3) the seeding tank bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.5%, yeast extract 2%, raw soya bean powder 3%, water 91.5%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the seeding tank of the bacterium of having gone out, start mixer, add cerelose more successively, dipotassium hydrogen phosphate, yeast extract, the raw soya bean powder, add the back stirring fully dissolved in 30-35 minute, mixer Revolution Per Minute 150 changes, close charging aperture then, the feeding steam is heated, when steam pressure in the seeding tank reaches the 0.14-0.15 MPa, keep just can reaching in 55-60 minute the effect of thorough sterilization, feed cooling water circulation then, when the culture medium in the seeding tank drops to 28 ℃-30 ℃, under aseptic condition, open the inoculation cap, insert the triangular flask liquid strain in seeding tank, each hundred kilograms of seed tank culture liquid inserts triangular flask liquid strain 1500-2000 milliliter, build the inoculation cap then, the beginning seed culture;
Condition of culture: the pH value nature, 28 ℃-30 ℃ of temperature, mixer Revolution Per Minute 120-140 changes, incubation time 30-35 hour;
(4) fermentation tank culture:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.2%, yeast extract 1%, raw soya bean powder 5%, corn flour 4%, wheat bran skin 8%, water 78.8%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the fermentation tank of slack tank sterilization, start mixer, Revolution Per Minute 150-180 changes, add the raw soya bean powder more successively, corn flour, yeast extract, the wheat bran skin, glucose, dipotassium hydrogen phosphate, add back continuation stirring and made it even in 30 minutes, the steam that feeds the 0.14-0.15 MPa then begins to heat, when steam pressure in the fermentation tank reaches the 0.14-0.15 MPa, keep just can reaching in 55-60 minute the effect of thorough sterilization, close steam then, feed the running water cooling by the jar interlayer, when temperature in the jar drops to 28 ℃-30 ℃, begin to connect the cultured Rhodotorula mucilaginose of seeding tank, at first improve seeding tank pressure when 0.1 MPa, fermentation tank tank pressure 0.05 MPa, open the inoculation pipeline valve, the seeding tank seed is transported in the fermentation tank goes, the seed consumption is between the 3%-5%;
Fermentation condition: pH value nature, 28 ℃-30 ℃ of temperature, mixer Revolution Per Minute 200-220 changes, cultivate and began to measure glycine content in 30 hours, measured once every two hours later on, when glycine content reaches 2.8%-3% in the zymotic fluid, (calculate) and just reach fermentation termination, can reach fermentation and finishing in general 40-45 hour with dry product;
(5) finished product extracts:
Zymotic fluid is warmed to 50 ℃-55 ℃, centrifugal with the yeast centrifuge, discard clear liquid and collect wet yeast, between 50 ℃-60 ℃, use the air blast drying machine drying, when moisture between 10%-12%, ultramicro grinding is finished product.
3. a kind of application that improves the feed addictive of collagen in the livestock and poultry muscle as claimed in claim 1 is characterized in that: add 0.5%-0.8% in livestock feed; In bird feed, add 0.6%-1%.
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| CN 201010134182 CN101779733B (en) | 2010-03-29 | 2010-03-29 | Feed additive for improving collagen in livestock and poultry muscle and production method thereof and application thereof |
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| CN102919598A (en) * | 2012-11-08 | 2013-02-13 | 黑龙江省轻工科学研究院 | Preparation method for feed additives enabling laying ducks to produce red-yolk duck eggs |
| CN107048057A (en) * | 2017-01-26 | 2017-08-18 | 张伟 | A kind of ginseng and lucid ganoderma nutrient health feed addictive and preparation method and application |
| CN107223799A (en) * | 2017-07-28 | 2017-10-03 | 烟台市昊星晟信息技术有限公司 | A kind of preparation method of health care chicken feed |
| CN114874940A (en) * | 2022-05-10 | 2022-08-09 | 吉林省农业科学院 | Complex microbial inoculant, and fermentation product and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919598A (en) * | 2012-11-08 | 2013-02-13 | 黑龙江省轻工科学研究院 | Preparation method for feed additives enabling laying ducks to produce red-yolk duck eggs |
| CN107048057A (en) * | 2017-01-26 | 2017-08-18 | 张伟 | A kind of ginseng and lucid ganoderma nutrient health feed addictive and preparation method and application |
| CN107223799A (en) * | 2017-07-28 | 2017-10-03 | 烟台市昊星晟信息技术有限公司 | A kind of preparation method of health care chicken feed |
| CN114874940A (en) * | 2022-05-10 | 2022-08-09 | 吉林省农业科学院 | Complex microbial inoculant, and fermentation product and application thereof |
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| CN101779733B (en) | 2012-12-26 |
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