CN101760433A - Method for culturing spiral seaweed by using waste water generated in production of purified water - Google Patents
Method for culturing spiral seaweed by using waste water generated in production of purified water Download PDFInfo
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- CN101760433A CN101760433A CN201010031347A CN201010031347A CN101760433A CN 101760433 A CN101760433 A CN 101760433A CN 201010031347 A CN201010031347 A CN 201010031347A CN 201010031347 A CN201010031347 A CN 201010031347A CN 101760433 A CN101760433 A CN 101760433A
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Abstract
The invention discloses a method for culturing spiral seaweed by using waste water from production of purified water, and aims to provide the method capable of reducing the culturing cost of the spiral seaweed, avoiding pollution of the waste water to a river and contributing to environmental protection. The method comprises the following steps: adjusting the pH value of the waste water from the production of the purified water to 7.5, after the micro boiling treatment, cooling the waste water to the room temperature; adding 0.5g of K2HPO4, 8g of NaHCO3 and 0.15ug of vitamin B12 in the waste water to perform the improved treatment, or adding 0.5g of K2HPO4, 8g of NaHCO3, 0.15mu g of vitamin B12, 1ml of A5 solution and 6ml of PIV metallic solution in the waste water per liter to perform the improved treatment; and adding the seaweed in an amount of 10 percent based on the volume of the improved waste water, and then culturing the spiral seaweed under the conditions of the illumination temperature of 24.8 DEG C, the illumination intensity of 40,001x and the light darkness period is 12h:12h. By using the method, various inorganic salts which are added into the fresh water are saved, and the culturing cost is greatly reduced.
Description
Technical field
The present invention relates to a kind of method of utilizing the waste water cultivation spirulina of producing the pure water generation.
Background technology
Spirulina is a protokaryon photosynthetic organism the most ancient on the earth, and the life cycle in existing so far 3,500,000,000 years is the abundantest, the isostatic natural green food of the nutrition found at present, and the title in " miniature green nourishing storehouse " is arranged.Have anti-cancer, press down cancer, radioprotective, regulating blood fat, plurality of health care functions such as reducing cholesterol, protection are cardiovascular, therefore, spirulina causes showing great attention to of national governments as food, medicine, healthcare products.Yet spirulina is a kind of algae, need a large amount of fresh water that it is cultivated, and the Freshwater resources of worsening shortages limits its industrial scale.
In recent years pure water output sharply rises, and accompanies it and the waste water of giving birth to also sharply increases.These waste water are poured the river into, not only cause waste, and change the salinity in river, influence environment.The waste water of producing the pure water generation contains a certain amount of inorganics (C, N, P source) and each metal ion species, and these inorganicss comprise that each metal ion species provides the basic nutrition condition for spirulina growth.Through suitably regulating, just can utilize the spirulina of these waste water production high values, thereby have great ecological significance and economic results in society, relevant patent and report are not also arranged at present.
Summary of the invention
The present invention is in order to overcome weak point of the prior art, a kind of method of utilizing the waste water cultivation spirulina of producing the pure water generation to be provided, to reduce the cultivation cost of spirulina, avoid waste water to the pollution that the river causes, helping protecting environment.
The present invention is achieved through the following technical solutions:
A kind of method of utilizing the waste water cultivation spirulina of producing the pure water generation is characterized in that, comprises the steps:
(1) will producing waste water that pure water produces, to adjust to pH be 7.5, through little processing of boiling, and cool to room temperature.
(2) in above-mentioned every liter of waste water, add 0.5g K
2HPO
4, 8g NaHCO
3, 0.15 μ g vitamins B
12Carry out improved treatment, perhaps in every liter of waste water, add 0.5gK
2HPO
4, 8g NaHCO
3, 0.15 μ g vitamins B
12, 1ml A
5Solution, 6ml PIV metallic solution carry out improved treatment, afterwards, are that 10% inoculum size inserts the algae kind according to the volume ratio of algae kind and improvement waste water, at illumination temperature is 24.8 ℃, intensity of illumination 4000lx, light dark period cultivate under the condition of 12h:12h, obtain spirulina.
Described spirulina is spirulina plalensis 869 algae strains.
The present invention has following technique effect:
Method of the present invention is by adjusting the waste water of producing the pure water generation, make it be applicable to the breed spirulina, saved like this and in fresh water, added plurality of inorganic salt, greatly reduce the cultivation cost of spirulina, and, avoid waste water to pour the river into and cause waste, for the cultivation of spirulina provides new approach.Simultaneously, can prevent pollution, protect environment, satisfy environmental requirement the river.
Description of drawings
Fig. 1 is optical density(OD) and dry cell weight relation curve.
Embodiment
Below in conjunction with specific embodiment to the detailed description of the invention.
Wherein, employed A
5The solution formula composition is as shown in table 1, and PIV metallic solution system component is as shown in table 2, and the AB culture medium prescription sees Table 3.
Table 1
Table 2
| Composition | Content (mg/L) |
| ??Na 2EDTA??FeCl 3·6H 2O??MnCl 2·4H 2O??ZnCl 2??CoCl 2·6H 2O??Na 2MoO 4·2H 2O | ??750??97??41??5??2??4 |
Table 3
| Composition | Content (g/L) |
| ??NaHCO 3??Na 2CO 3??K 2HPO 4??NaNO 3??K 2SO 4??NaCl??MgSO 4·7H 2O??CaCl 2·2H 2O PIV metallic solution A 5The solution vitamins B 12 | ??13.61g/L??4.03g/L??0.50g/L??2.50g/L??1.00g/L??1.00g/L??0.20g/L??0.04g/L??6ml/L??1ml/L??0.15μg/L |
Embodiment 1
Tianjin Beichen District pure water factory, the initial pH 7.5 of the waste water that the production pure water produces, specific conductivity 1107 μ s/cm, colourless, through little processing of boiling, cool to room temperature adds 0.5gK in every liter of waste water afterwards
2HPO
4, 8gNaHCO
3, 0.15 μ g vitamins B
12Carry out improved treatment.
The spirulina plalensis nutrient solution of logarithmic phase will be cultured to, shake up, volume ratio according to algae kind and improvement waste water is that 10% inoculum size inserts in the improvement waste water, be placed on then in the HPG-280BX illumination box, illumination temperature is 24.8 ℃, and illumination progression is 4000lx, and the light dark period of SPIRULINA CULTIVATION is to cultivate under the condition of 12h:12h, sampling every day sees Table 4 at the absorbance that 560nm measures each test group.The result who cultivates the spirulina after 7 days is as shown in table 6.
Embodiment 2
Tianjin Beichen District pure water factory, the initial pH 7.5 of the waste water that the production pure water produces, specific conductivity 1107 μ s/cm, colourless, through little processing of boiling, cool to room temperature, every liter adds 0.5gK afterwards
2HPO
4, 8gNaHCO
3, 0.15 μ g vitamin B12,1ml A
5Solution, 6mlPIV metallic solution carry out improved treatment.
The spirulina plalensis nutrient solution of logarithmic phase will be cultured to, shake up, according to the volume ratio of algae kind and improvement waste water is in the waste water after 10% inoculum size inserts improvement, be placed on then in the HPG-280BX illumination box, illumination temperature is 24.8 ℃, and illumination progression is 4000lx, the light dark period of SPIRULINA CULTIVATION is to cultivate under the condition of 12h:12h, cultivated 7 days, sampling every day sees Table 5 at the absorbance that 560nm measures each test group.The result who cultivates the spirulina after 7 days is as shown in table 6.
Table 4
Table 5
Table 6
| The substratum composition | OD value after 7 days | Dry cell weight after 7 days (g/L) | Algae color after 7 days | Algae form after 7 days | |
| Embodiment 1 | ??0.5gK 2HPO 4，??8gNaHCO 3, the 1ml vitamins B 12, 1L waste water | ??1.713 | ??1.601 | Dark green | Thicker |
| Embodiment 2 | ??0.5gK 2HPO 4，??8gNaHCO 3, the 1ml vitamins B 12, 1ml As liquid, 6mlPIV metallic solution, 1L waste water | ??1.886 | ??1.766 | Dark green | Thicker |
| The AB medium controls | ??NaHCO 3?13.61g，??Na 2CO 3?4.03g，??K 2HPO 4?0.50g，??NaNO 3?2.50g，??K 2SO 4?1.00g，??MgSO 4·7H 2O?1.00g，??NaCl?0.20g，??CaCl 2·2H 2O 0.04g, PIV metallic solution 6ml, A 5Solution 1ml, vitamins B 120.15 μ g 993ml distilled water | ??1.802 | ??1.688 | Dark green | Thicker |
Under same experiment condition, promptly in the HPG-280BX illumination box, illumination temperature is 24.8 ℃, and illumination progression is 4000lx, and the light dark period of SPIRULINA CULTIVATION is to cultivate under the condition of 12h:12h, cultivated 7 days, and be the blank group with the AB substratum.Measure the relation between different incubation time frustule dry weights and the 560nm absorbance, obtain optical density(OD) and dry cell weight relation curve as shown in Figure 1, obtain equation of linear regression from figure: Y=10.664X+0.0024.Y-absorbance in the formula; X-dry cell weight value.Thereby the dry weight of trying to achieve embodiment 1 and embodiment 2 frustules is 1.601g/L and 1.766g/L, with the AB substratum be that the dry weight 1.688g/L of frustule of blank group is close, and saved 7-9 kind medium component, greatly reduce the cultivation cost of spirulina.The present invention utilizes and produces the waste water cultivation spirulina that pure water produces, and cultivates spirulina for mass-producing a kind of feasible method is provided, and has potential economic results in society and ecological significance.
Claims (2)
1. a method of utilizing the waste water cultivation spirulina of producing the pure water generation is characterized in that, comprises the steps:
(1) will producing waste water that pure water produces, to adjust to pH be 7.5, through little processing of boiling, and cool to room temperature;
(2) in above-mentioned every liter of waste water, add 0.5g K
2HPO
4, 8g NaHCO
3, 0.15 μ g vitamins B
12Carry out improved treatment, perhaps in every liter of waste water, add 0.5gK
2HPO
4, 8g NaHCO
3, 0.15 μ g vitamins B
12, 1ml A
5Solution, 6ml PIV metallic solution carry out improved treatment, afterwards, are that 10% inoculum size inserts the algae kind according to the volume ratio of algae kind and improvement waste water, at illumination temperature is 24.8 ℃, intensity of illumination 4000lx, light dark period cultivate under the condition of 12h: 12h, obtain spirulina.
2. the method for the waste water cultivation spirulina of pure water generation is produced in utilization according to claim 1, it is characterized in that described spirulina is spirulina plalensis 869 algae strains.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102940284A (en) * | 2012-09-01 | 2013-02-27 | 山东博然螺旋藻生物股份有限公司 | Preparation method of zinc-rich instant spirulina |
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2010
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102940284A (en) * | 2012-09-01 | 2013-02-27 | 山东博然螺旋藻生物股份有限公司 | Preparation method of zinc-rich instant spirulina |
| CN102940284B (en) * | 2012-09-01 | 2013-07-31 | 山东博然螺旋藻生物股份有限公司 | Preparation method of zinc-rich instant spirulina |
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Open date: 20100630 |