CN101759270B - Method for removing heavy metal cadmium from water solution - Google Patents
Method for removing heavy metal cadmium from water solution Download PDFInfo
- Publication number
- CN101759270B CN101759270B CN 200810185075 CN200810185075A CN101759270B CN 101759270 B CN101759270 B CN 101759270B CN 200810185075 CN200810185075 CN 200810185075 CN 200810185075 A CN200810185075 A CN 200810185075A CN 101759270 B CN101759270 B CN 101759270B
- Authority
- CN
- China
- Prior art keywords
- cadmium
- dry powder
- shi
- heavy metal
- shi yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 229910052793 cadmium Inorganic materials 0.000 title claims abstract description 41
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 20
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 25
- WLZRMCYVCSSEQC-UHFFFAOYSA-N cadmium(2+) Chemical compound [Cd+2] WLZRMCYVCSSEQC-UHFFFAOYSA-N 0.000 claims abstract description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 39
- 239000007788 liquid Substances 0.000 claims description 24
- 239000007864 aqueous solution Substances 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 10
- 238000001179 sorption measurement Methods 0.000 claims description 6
- 230000003068 static effect Effects 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 5
- 230000010355 oscillation Effects 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 3
- 239000003651 drinking water Substances 0.000 abstract description 2
- 235000020188 drinking water Nutrition 0.000 abstract description 2
- 241000235033 Zygosaccharomyces rouxii Species 0.000 abstract 4
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000010865 sewage Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 10
- 238000004321 preservation Methods 0.000 description 7
- 230000004913 activation Effects 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000009514 concussion Effects 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- 240000006063 Averrhoa carambola Species 0.000 description 2
- 235000010082 Averrhoa carambola Nutrition 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000010842 industrial wastewater Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 235000015067 sauces Nutrition 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 235000013555 soy sauce Nutrition 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001149258 Sporobolus alterniflorus Species 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012716 precipitator Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 231100000701 toxic element Toxicity 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Landscapes
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method capable of removing heavy metal cadmium from a water solution, which comprises the following steps: preparing zygosaccharomyces rouxii dry powder by zygosaccharomyces rouxii; then, putting the zygosaccharomyces rouxii dry powder into a cadmiferous solution; controlling the proper conditions; and removing the cadmium from the water solution through removing the zygosaccharomyces rouxii absorbed with cadmium ions. The invention has the advantages of simple process, high removal efficiency, low cost, high safety, no secondary pollution and the like. The invention has great significance on the removal of the cadmium ions from the water, and has wide application prospects in the fields of sewage treatment, drinking water purification, food production and biochemical industry.
Description
Technical field
The present invention relates to a kind of method that removes heavy metal cadmium in the aqueous solution, belong to technical field of food biotechnology.
Background technology
Cadmium is the toxic elements in human body element.Environmental pollution causes water pollution, cause some food raw material especially in the fishery products cadmium content exceed standard, threatening human health.The present cadmium method of taking off mainly contains the precipitator method etc.Advantages such as mikrobe Yin Qiyi cultivates, breeding is fast and become research and the application direction that removes heavy metal.
The Lu Shi yeast is prone to cultivate, and is the main starter on the fermentative prodn such as soy sauce and sauce.The present invention utilizes the Lu Shi yeast to the favourable absorption property of cadmium and the sedimentation effect of itself, from contain the cadmium aqueous solution, removes cadmium ion.The Lu Shi yeast cell prepares through microbial fermentation technology and Freeze Drying Technique, obtains exsiccant, anti-transportation, is prone to the solid of preservation.Put into the solution that contains the finite concentration cadmium ion, the CONTROL PROCESS condition removes heavy metal cadmium, for containing the cadmium WWT a kind of method is provided.
The patented process of handling industrial waste water containing Cd at present has: CN200810011100.9 and CN200610017040.2 are and utilize beer waste yeast, and CN200610057596.4 adopts a kind of bacillus cereus that takes off cadmium as biological adsorption agent.CN200710030649.8 utilizes xylophyta carambola (Averrhoa carambola L.), activation and absorb contaminated soil or water body in cadmium.CN200810124261.9 spartina alterniflora loisel gac also can be administered cadmium wastewater.CN200510031203.8 method of utilizing mushroom culture medium waste to remove industrial waste water containing Cd.CN94106775.0, CN99103774.X and CN00131216.2 all belong to and relate to the method that adds the heavy metals such as cadmium in the chemical reagent deposition trade effluent.CN03125232.X relates to a kind of method of utilizing rock pit wood material to remove arsenic and cadmium in the waste water.
About Lu Shi zymic patent has: the Lu Shi yeast that CN200610043899.0 and CN200510045226.4 relate to is all as one of soy sauce and sweet fermented flour sauce flavouring starter; The Lu Shi yeast that CN01116463.8 and CN99124706.X relate to is all as being microbial starter culture.
Do not see at present and adopt Lu Shi yeast dry powder to remove the patented process of the heavy metal cadmium in the aqueous solution.
Advantage of the present invention is, Lu Shi yeast dry powder is easy to preparation, and it is simple to take off cadmium technology, high adsorption capacity and fast; Yeast is prone to and water sepn, except that cadmium efficient is high, cost is low, security good, do not cause secondary pollution etc.Cadmium ion to removing in the aqueous solution is significant, as in WWT, purifying drinking water source, contain fields such as the protein enzyme solution of cadmium, the herbal medicine decocting liquid that contains cadmium and biochemical industry very wide application prospect arranged.
Summary of the invention
The objective of the invention is provides a kind of Lu Shi of application yeast dry powder to remove the method for the heavy metal cadmium in the aqueous solution for the defective that overcomes prior art.
This takes off the cadmium method: be inoculated in wort or the YEPD substratum commonly used 20～38 ℃ of constant temperature culture, collecting cell behind the Lu Shi activated yeast with preservation (commercial back preservation cryogenic refrigerator in); After the freeze-drying; Be prepared into yeast dry powder, dry powder put into contained in the heavy metal cadmium aqueous solution then, the control adsorption conditions adsorbs; Leave standstill, make the yeast natural subsidence.
Lu Shi yeast dry powder is put into and is contained finite concentration (in 0.01～200mg/L) the cadmium-ion solution; Make and add that cell concentration is 0.1～9.5g/L behind the Lu Shi yeast dry powder; 0～40 ℃ of adjustment solution temperature; Regulate the pH value at 4～7 scope, the Clothoid type oscillation frequency 0～100rpm of adsorption process, treatment time 5～60min; Static 60～180min, yeast sedimentation is separated and is removed the Lu Shi yeast that is adsorbed with cadmium ion, can reach to remove the cadmium more than 80% in the solution.
Embodiment
The Lu Shi barms of preservation after 28～30 ℃ of activation of wort or YEPD solid inclined-plane, is prepared into wort or YEPD liquid seeds commonly used, is inoculated in the YEPD fermention medium by inoculum size 2～10%; Liquid amount is 5～60%, 20～38 ℃ of constant temperature, rotating speed 100～300rpm; Cultivate 10～48h, with the centrifugal 10～30min of the centrifugal 1500～6000rpm of fermented liquid, collecting cell and with behind the distilled water wash; Freeze-drying grinds and screening, is prepared into Lu Shi yeast dry powder.Lu Shi yeast dry powder is put in the solution that contains heavy metal cadmium; Be processed liquid concentration of cadmium ions 0.01～200mg/L; Add that cell concentration is 0.1～9.5g/L behind the Lu Shi yeast dry powder, oscillation frequency is at 0～100rpm, 1～40 ℃ of temperature; The pH value that adjustment contains cadmium liquid is 4～7, adsorption time 1～60min; Remove the Lu Shi yeast that is adsorbed with cadmium behind static 60～180min.
Embodiment 1
The Lu Shi barms of preservation after 28 ℃ of activation of YEPD solid inclined-plane constant temperature, is prepared into liquid seeds commonly used, liquid seeds is inoculated in the YEPD fermention medium inoculum size 5%; Liquid amount is 50%, 30 ℃ of constant temperature, rotating speed 180rpm; Cultivate 12h, with the centrifugal 10min of fermented liquid 4200rpm, collecting cell is also used distilled water wash; After the lyophilize, grind and screening, be prepared into Lu Shi yeast dry powder.Reach the amount of 5g/L by adding cell concentration behind the Lu Shi yeast dry powder, Lu Shi yeast dry powder is put into contained in the 10mg/L cadmium solution, the pH value that adjustment contains cadmium liquid is 5.0, room temperature, and 30min is adsorbed in concussion once in a while; Behind the static 60min, separate and remove the Lu Shi yeast that is adsorbed with cadmium.
Embodiment 2
The Lu Shi barms of preservation after 30 ℃ of activation of wort solid inclined-plane constant temperature, is prepared into wort liquid seed, liquid seeds is inoculated in the YEPD fermention medium inoculum size 7%; Liquid amount is 40%, 28 ℃ of constant temperature, rotating speed 160rpm; Cultivate 22h, with the centrifugal 20min of fermented liquid 3000rpm, collecting cell is also used distilled water wash; After the lyophilize, grind and screening, be prepared into Lu Shi yeast dry powder.Reach the amount of 4g/L by adding cell concentration behind the Lu Shi yeast dry powder, Lu Shi yeast dry powder is put into contained in the 50mg/L cadmium solution, the pH value that adjustment contains cadmium liquid is 6.0,30 ℃ of temperature, and concussion frequency 100rpm adsorbs 20min; Behind the static 120min, separate and remove the Lu Shi yeast that is adsorbed with cadmium.
Embodiment 3
The Lu Shi barms of preservation after 29 ℃ of activation of wort solid inclined-plane constant temperature, is prepared into YEPD liquid seeds commonly used, liquid seeds is inoculated in the YEPD fermention medium inoculum size 6%; Liquid amount is 20%, 35 ℃ of constant temperature, 150rpm; Cultivate 24h, with the centrifugal 30min of fermented liquid 3500rpm, collecting cell is also used distilled water wash; After the lyophilize, grind and screening, be prepared into Lu Shi yeast dry powder; Reach the amount of 4.5g/L according to adding cell concentration behind the Lu Shi yeast dry powder, Lu Shi yeast dry powder is put into contained in the 100mg/L cadmium solution, it is 7.0 that adjustment contains the cadmium liquid pH value; 10 ℃ of temperature, concussion frequency 60rpm, absorption 20min; Behind the static 180min, separate and remove the Lu Shi yeast that is adsorbed with cadmium.
Claims (1)
1. method that removes heavy metal cadmium in the aqueous solution is characterized in that the preparation of Lu Shi yeast dry powder and removes the method for heavy metal cadmium in the aqueous solution, the steps include: the Lu Shi activated yeast that the inclined-plane is preserved and is prepared into seed liquor; Get an amount of seed liquor and be inoculated into and be equipped with in the yeast culture base of having sterilized, 20～38 ℃ of constant temperature are 100～300r/min down, liquid amount 5～60%; Cultivate 10～48h; Fermented liquid is collected thalline and washing through the centrifugal 5～30min of 1500～6000r/min, and lyophilize is pulverized; Screening is prepared into Lu Shi yeast dry powder; Utilize Lu Shi yeast dry powder to remove that the condition that removes of heavy metal cadmium is in the aqueous solution: solution temperature is 1～50 ℃; The pH value is in 4～7 scope; Oscillation frequency 0～150r/min in the adsorption process; Be processed liquid concentration of cadmium ions 0.01～200mg/L, cell concentration is 0.1～9.5g/L behind the adding Lu Shi yeast dry powder, treatment time 5～60min; Static then 60～180min removes the Lu Shi yeast that is adsorbed with cadmium ion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810185075 CN101759270B (en) | 2008-12-25 | 2008-12-25 | Method for removing heavy metal cadmium from water solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810185075 CN101759270B (en) | 2008-12-25 | 2008-12-25 | Method for removing heavy metal cadmium from water solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101759270A CN101759270A (en) | 2010-06-30 |
| CN101759270B true CN101759270B (en) | 2012-12-05 |
Family
ID=42490676
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200810185075 Expired - Fee Related CN101759270B (en) | 2008-12-25 | 2008-12-25 | Method for removing heavy metal cadmium from water solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101759270B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102070252B (en) * | 2010-11-29 | 2012-02-08 | 湖南大学 | Application of thioacetamide in aspect of promoting extracellular cadmium crystalline particles to aggregate and grow |
| CN102674494A (en) * | 2012-04-28 | 2012-09-19 | 华南农业大学 | Method for recovering heavy metal cadmium ions in water by using shells of apple snails |
| CN104489489B (en) * | 2015-01-12 | 2017-05-03 | 湖南农业大学 | Method for reducing heavy metal cadmium in rice by utilizing mixed fermentation of lactobacillus and saccharomycetes |
| CN111925950A (en) * | 2020-03-17 | 2020-11-13 | 徐州工程学院 | High-cadmium-resistance strain and separation and screening method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003099730A1 (en) * | 2002-05-23 | 2003-12-04 | Minaki Advance Co., Ltd. | Water clarifying agent inducing catalytic reaction and method of clarifying water through activation of microorganism |
| CN1900271A (en) * | 2005-07-22 | 2007-01-24 | 国家海洋局第三海洋研究所 | Ocean salt monad and its using method in biological flocculation and heavy metal adsorption |
| CN101082035A (en) * | 2006-06-01 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of dotey bacterium and method for restoring shellfish culture environment |
-
2008
- 2008-12-25 CN CN 200810185075 patent/CN101759270B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003099730A1 (en) * | 2002-05-23 | 2003-12-04 | Minaki Advance Co., Ltd. | Water clarifying agent inducing catalytic reaction and method of clarifying water through activation of microorganism |
| CN1900271A (en) * | 2005-07-22 | 2007-01-24 | 国家海洋局第三海洋研究所 | Ocean salt monad and its using method in biological flocculation and heavy metal adsorption |
| CN101082035A (en) * | 2006-06-01 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of dotey bacterium and method for restoring shellfish culture environment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101759270A (en) | 2010-06-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104585563B (en) | It is a kind of to utilize the method for compounding heavy metal cadmium in lactobacillus-fermented removing rice | |
| CN102731176B (en) | Production method for seaweed bio-composite organic liquid fertilizer | |
| CN106145545B (en) | A kind of livestock breeding wastewater processing method | |
| CN101759270B (en) | Method for removing heavy metal cadmium from water solution | |
| CN105624200A (en) | Recycling method of hirsutella sinensis fermentation filtrate | |
| CN110195026B (en) | Preparation of DEHP degrading microbial inoculum and method for effectively reducing DEHP pollution in vegetable production | |
| CN104725136B (en) | One marine organisms fertilizer for growing tobacco special and its preparation method and application | |
| CN102086443B (en) | Microorganism combined with plant materials for removing cadmium in industrial wastewater and method for removing cadmium by using same | |
| CN102965412A (en) | Trehalose preparation method | |
| CN104694419B (en) | A kind of efficiently lead ion biological adsorption agent and preparation method and application | |
| Nagase et al. | Selective cadmium removal from hard water using NaOH-treated cells of the cyanobacterium Tolypothrix tenuis | |
| CN103468269A (en) | Preparation method of material for restoring soil polluted by heavy metal | |
| CN101337731B (en) | Method for removing cadmium in industrial wastewater by bio adsorbing agent | |
| CN105668805B (en) | Utilize the method for cadmium in biomass-microorganism collaboration removal waste water | |
| CN103695490B (en) | High-purity arginine production process | |
| CN102220296B (en) | Method for extracting garlic superoxide dismutase from garlic sheet processing waste water | |
| CN110026060A (en) | A kind of biomaterial and its making and use method of degradation of formaldehyde | |
| CN102070252B (en) | Application of thioacetamide in aspect of promoting extracellular cadmium crystalline particles to aggregate and grow | |
| CN101851588A (en) | Culture method for improving adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium | |
| CN107307337A (en) | A kind of chlorella tablet and preparation method thereof | |
| CN102174506B (en) | Method for quickly suppressing activity of acidic trehalose lytic enzyme in beer yeast cell | |
| CN108636373A (en) | A kind of fungi is modified the preparation method of biogas residue heavy metal absorbent | |
| CN109422414B (en) | Environment-friendly process for treating glucose industrial wastewater | |
| CN111194905A (en) | Process method for extracting health-care biological salt from sea asparagus | |
| CN103464106B (en) | Preparation method of biomass Congo red dye absorbing material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121205 |