CN101755754B - Bactericidal composite taking fluazinam as main ingredient - Google Patents

Abstract

The invention discloses a bactericide composite, which consists of active ingredients of fluazinam (A) and bromothalonil (B), and the mass ratio of A to B is 1:100 to 100:1. The composite has obvious synergy and high prevention effect, and is suitable for controlling diseases on fruits and vegetables, particularly for controlling anthracnose.

Description

A kind of bactericidal composition take fluazinam as main component

Technical field

The present invention relates to a kind of bactericidal composition, relate in particular to a kind of bactericidal composition take fluazinam and bromothalonil as main component.

Background technology

Anthracnose be generally occur in a kind of world wide, the heavier disease that causes harm, can cause that fallen leaves, branch are withered, fruit rot and shedding.Occur to rot during fruit is everlasting and is stored and transported in spite of illness, thus be again a kind of important postharvest disease, such as citrus anthracnose, mango anthracnose etc.Anthrax bacteria is survived the winter in the residual body of host or soil with mycelium, conidium or aceravlus.Conidium is by wind and rain, the propagation such as water, and can infect from wound etc.High humidity, sick section be moistening, water droplet or moisture film are arranged is that pathogen produces a large amount of conidial essential condition, and cloudy weather for several days running morbidity in season is heavier.Citrus anthracnose outburst in 2008 causes a large amount of citrus orchards in ground such as North Guangdong, west of Guangdong Province Yuexi to ruin the garden, causes heavy losses for local orchard worker.

Fluazinam belongs to 2,6-dinitroanilines, and the broad spectrum protection bactericide is very effective to Alternaria, Botrytis, Phytophthora, Plasmopara, Sclerotinia and Venturia germ.English common name: Fluazinam chemical name: 3-chloro-N-(3-chloro-5-trifluoromethyl-2-pyridine radicals)-α, α, α-three fluoro-2,6-dinitro para-totuidine molecular formula: C ₁₃H ₄Cl ₂F ₆N ₄O ₄The concrete disease that fluazinam can be prevented and treated has gray mold of cucumber, downy mildew, anthracnose, powdery mildew, tomato late blight, apple scab, leaf spot, Pear black spot, rust, rice blast, banded sclerotial blight, crown hull sugar, grape grey mould, downy mildew, citrus scab, gray mold, potato late blight, lawn spot disease etc.Anthrax pathogen to anti-benzimidazole type and triazole bactericidal agent also has good result, and the fluazinam resistance of rainwater washing against, and the lasting period is long.

But the production that fluazinam is present and use cost are all higher, and long-term separately use also has very large resistance risk.

The combination of the pesticide active ingredient of fluazinam and other structure being carried out composite, is a kind of effective mode that solves cost and resistance problem in its single dose application process.The pesticide active ingredient of different structure shows three kinds of type of action, i.e. summation action, synergistic effect and antagonism after mixing usually.Compound synergic is well filled a prescription, and can improve actual control efficiency, reduces the usage amount of agricultural chemicals, also helps to delay the drug-fast generation of germ, is the important means of integrated control.

Summary of the invention

The purpose of this invention is to provide a kind of synergistic effect obvious, to the bactericidal composition that the anthracnose control efficiency is good, cost is low, resistance risk is low.

Another object of the present invention provides the application of this bactericidal composition.

For overcoming the deficiencies in the prior art, technical scheme of the present invention is to solve like this: a kind of bactericidal composition, contain active ingredient fluazinam (A) and bromothalonil (B), A and B mass ratio are 1: 100～100: 1, and preferred proportion is 1: 40～40: 1.

Bromothalonil (English common name: bromothalonil, chemical molecular formula C ₆H ₆Br ₂N ₂Have another name called tektamer), it is the bactericide of a kind of wide spectrum, efficient, low toxicity, can suppress and root out the growth of fungi, bacterium, algae, the crops multiple diseases there is preferably control efficiency, especially anthracnose there is special efficacy, to crop safety, the various crop such as fruit tree, grape, vegetables, cotton, peanut, watermelon, tobacco, tea tree, flowers be can be widely used in, multiple fungoid, the bacterial diseases such as anthracnose, scab, shot hole, powdery mildew, rust, damping off, damping off, rhizome maize ear rot, canker, bacterial wilt, angular leaf spot prevented and treated.Have unique protection, interior suction treatment and shoveling function, effect is remarkable, and the lasting period is long.This compound can be absorbed by somatic cells rapidly, and conducts in somatic cells, and disturbs the normal development of somatic cells, thereby reaches antibacterial, bactericidal action.And can stimulate the activity of making plurality of enzymes in the object, and increase photosynthesis, improve crop yield.Application mode is flexible, and foliar spray, seed treatment and soil are filled with root, all show preferably preventive effect.Also be applicable to the algae removal that lake environment is processed, improve fishing pool water quality; Textile, leather etc. are anticorrosion, gas defence etc.

The active ingredient of said composition and auxiliary agent and filler can be processed into the upper any formulation that allows of agricultural, and formulation has missible oil, microemulsion, aqueous emulsion, suspending agent, wetting powder, water dispersible granules preferably.

In the bactericidal composition of the present invention, the mass accumulation percentage composition of A and B is 5%～80%.

The formulation of above-mentioned bactericidal composition also comprises auxiliary agent and other material that helps drug effect to bring into play except comprising effective constituents A and B.Missible oil comprises organic solvent, cosolvent and emulsifier; Microemulsion comprises organic solvent, emulsifier and water; Aqueous emulsion comprises organic solvent, dispersant, emulsifier and water; Suspending agent comprises water, dispersant, thickener, antifreezing agent, wetting agent, defoamer; Wetting powder comprises wetting agent, dispersant and filler; Water dispersible granules comprises dispersant, disintegrant, filler and wetting agent.Below all be various compositions commonly used in the pesticidal preparations or that permission is used, be not particularly limited, concrete composition and consumption require by simple experiment definite according to prescription.For example:

Organic solvent can be selected one or more in isopropyl alcohol, dimethylbenzene, DMF, ring ethyl ketone, toluene, dimethyl sulfoxide (DMSO), methyl alcohol, ethanol, trimethyl cyclic ethylene ketone, NOP, 1-METHYLPYRROLIDONE, propyl alcohol, butanols, ethylene glycol, diethylene glycol, ethylene glycol monomethyl ether, butyl ether, ethyl acetate, the vegetable oil.

Cosolvent can be selected one or more in 1-METHYLPYRROLIDONE, dimethyl formamide, ethanol, the third ethanol, the acetone.

Dispersant can be selected sodium lignin sulfonate, calcium lignosulfonate, pulls open powder, in the calcium dodecyl benzene sulfonate, polycarboxylate, alkyl benzene sulfonate calcium salt, mersolates, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, fatty acid glyceride APEO one or more.

Emulsifier can be selected agricultural emulsifier NO．600 (phenyl phenol polyoxy ethyl ether), No. 1601 (phenethyl phenol polyoxy ethyl polypropylene-base ether) of farming breast, No. 500 (alkyl benzene calcium sulfonate) of farming breast, OP series phosphate (polyoxyethylene nonylphenol ether phosphate), No. 600 phosphates (phenyl phenol polyoxy ethyl ether phosphate), styrene polyoxyethylene rice ammonium sulfate, alkyl biphenyl ether disulfonic acid magnesium salts, triethanolamine salt, No. 400 (benzyl xylenol polyoxy ethyl ether) of farming breast, Nongru-700 number (alkyl phenolic resin polyoxy ethyl ether), No. 36 (phenethyl fluosite polyoxy ethyl ether) of peaceful breast, No. 1600 (phenethyl phenol polyoxy ethyl polypropylene-base ether) of farming breast, ethylene oxide-propylene oxide block copolymer, OP series (polyoxyethylene nonylphenol ether), No. 33 (alkylaryl polyoxyethylene polyoxypropylene ether) of farming breast, No. 34 (alkylaryl polyoxyethylene poly-oxygen propylene aether) of farming breast, sapn series (sorbitan monostearate), tween series (sorbitan fatty acid ester APEO), in the AEO series (the pure APEO of fat) one or more.

Antifreezing agent can be selected one or more in glycerine, urea, ethylene glycol, the propane diols.

Wetting agent can select that methyl naphthalene sulfonate formaldehyde condensate, lauryl sodium sulfate, Negel, alkyl benzene calcium sulfonate, tea are withered, spaonin powder, silkworm excrement, soapberry powder, bay alcohol radical sodium sulphate, washing powder, pull open in the powder one or more.

Disintegrant can be selected one or more in ammonium sulfate, urea, bentonite, aluminium chloride, citric acid, succinic acid, the sodium bicarbonate.

Thickener can be selected in xanthans, CMC, methylcellulose, Magnesiumaluminumsilicate, the polyvinyl alcohol one or more.

Defoamer such as organic silicon defoamer.

Filler can be selected one or more in White Carbon black, mountain range soil, precipitated calcium carbonate, talcum, imvite or protruding Attapulgite, float stone, brickbat, sepiolite or bentonite and non-adsorptivity calcium soil or the sand.

Product described in the invention can the finished product preparation form provide, and namely each material mixes in the composition, and the composition of composition also can provide with the single dose form, directly directly mixes in bucket or tank before the use, then is diluted to required concentration.

Bactericidal composition of the present invention can be used for preventing and treating the multiple diseases on the crops such as cucumber, watermelon, pumpkin, muskmelon, strawberry, capsicum, Chinese cabbage, eggplant, potato, grape, control is particularly useful for preventing and treating anthracnose because of fungus-caused plant diseases such as oomycetes, imperfect fungus, sac fungi, basidiomycetes.Composition of the present invention can be used by common method, and such as cast, injection, spraying, dusting, scatter or be fuming, its amount of application is with weather condition or crop state variation.

The present invention compared with prior art, its advantage is: 1, the composition synergistic effect is obvious, preventive effect is compared with single dose and is significantly improved; 2, after drug effect improved, the effective ingredient consumption in field descended, and has reduced production and use cost, has reduced residue of pesticide and environmental pollution; 3, composition is comprised of the active ingredient of different mechanism of action, and action site increases, and is conducive to overcome and delays the drug-fast generation of germ.

Embodiment

The pesticide active ingredient of different types of structure being carried out composite, is a kind of effective means that solves at present the problem such as cost and resistance in the agricultural chemicals single dose application process.The pesticide active ingredient of different types of structure shows three kinds of type of action after mixing usually, and namely summation action, synergistic effect and antagonism act on but which kind of is specially, and be unpredictable, only has by lot of experiments and just can know.Compound synergic is well filled a prescription, and can improve actual control efficiency, reduces the usage amount of agricultural chemicals, helps the generation of delaying drug resistance, is the important means of scientific prevention and cure damage by disease and insect.

The present composition has obvious synergistic function to the plant anthrax bacteria, and is not only the simple addition of two kinds of pharmacy effects, and this can be clear that from the result of following toxicity test experiment very much.

Biologicall test example 1: composition is to the Toxicity Determination of citrus anthracnose bacterium

Subjects is citrus anthracnose bacterium (Colletotrichum gloesporioides Penz).

The mycelial growth rate method is adopted in test.Getting experiment liquid that 5ml prepares joins the hot medium of 45ml (PDA medium is housed, 45-50 ℃) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, each culture dish is poured band medicine medium 12ml into, each processes 4 repetitions, and level leaves standstill, the cooling after flat board.Cut bacterium cake from what cultivate 5d for examination bacterium edge with diameter 5mm card punch, will receive with the one side of mycelia and be with on the malicious medium with choosing pin, all operations all carries out sterile working at superclean bench.After the processing flat board is placed in 25 ℃ the constant temperature aseptic culture case and cultivates, adopt the right-angled intersection method to measure respectively the colony diameter that each is processed behind the 5d, calculate and respectively process the mean value of colony diameter, average net growth and the mycelial growth inhibition rate of colony diameter.

Net growth (mm)=measurement colony diameter-5

Mycelial growth inhibition rate (%)=[(control group net growth-processed group net growth)/control group net growth] * 100

Mycelial growth inhibition rate is converted into probability value (y), and liquor strength (μ g/ml) converts logarithm value (x) to, calculates concentration EC in virulence equation and the inhibition with method of least squares ₅₀, according to toxicity index and the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun medicament.

Actual measurement toxicity index (ATI)=(standard medicament EC ₅₀/ reagent agent EC ₅₀) * 100

The percentage composition of B in the percentage composition of A+B medicament toxicity index * mixture in theoretical toxicity index (TTI)=A medicament toxicity index * mixture

Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (the ATI)/theoretical toxicity index (TTI) of mixture] * 100

Evaluation criterion: CTC≤80 are antagonism, and 80＜CTC＜120 are summation action, and CTC 〉=120 are synergistic effect.

As can be seen from Table 1, fluazinam and bromothalonil were mixed in 1: 100 in mass ratio～100: 1, and the citrus anthracnose bacterium is all had significant synergistic effect, and when especially proportioning was between 1: 40～40: 1, synergy was more remarkable, and co-toxicity coefficient is all more than 200.

Table 1 fluazinam and bromothalonil are mixed to citrus anthracnose bacterium Toxicity Determination result

Process	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
					Fluazinam (A)	0.25	100	/	/
Bromothalonil (B)	0.82	30.49	/	/
					A∶B(100∶1)	0.17	147.06	99.31	148.08
A∶B(70∶1)	0.19	131.58	99.02	132.88
					A∶B(40∶1)	0.11	227.27	98.30	231.19
A∶B(15∶1)	0.13	192.31	95.66	201.04
					A∶B(1∶1)	0.18	138.89	65.24	212.88
A∶B(1∶15)	0.35	71.43	34.83	205.06
					A∶B(1∶40)	0.38	65.79	32.18	204.42
A∶B(1∶70)	0.54	46.29	31.47	147.13
					A∶B(1∶100)	0.65	38.46	31.18	123.37

In order to make purpose of the present invention, technical scheme and advantage clearer, the present invention describes with following concrete formulation examples, but the present invention is limited to absolutely not these examples.The following stated only is preferably embodiment of the present invention, only in order to explain the present invention, can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that all any modifications of making within the spirit and principles in the present invention, be equal to replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.

Missible oil embodiment

Pesticide activity component, emulsifier, partial solvent are mixed in proportion, and mix and blend in modulation kettle until completely dissolved, adds remaining solvent more lentamente, obtains uniform oil phase after fully stirring, and makes the missible oil product.

Example of formulations 1,20.2% fluazinam bromothalonil missible oil

Fluazinam 20%, bromothalonil 0.2%, farming breast No. 500 2% (emulsifier), dimethylbenzene (solvent) are supplemented to 100%.

Example of formulations 2,30.3% fluazinam bromothalonil missible oil

Fluazinam 0.3%, bromothalonil 30%, agricultural emulsifier NO．600 4% (emulsifier), vegetable oil (solvent) are supplemented to 100%.

Example of formulations 3,30% fluazinam bromothalonil missible oil

Fluazinam 15%, bromothalonil 15%, farming breast No. 500 3% (emulsifier), vegetable oil (solvent) are supplemented to 100%.

Microemulsion, aqueous emulsion embodiment

By each component proportion, solubilization of active ingredient in solvent and cosolvent, is added surfactant, mix and make oil phase.Water-soluble component and water mixing are made water, oil phase is added aqueous phase or water again and add in the oil phase, the limit edged stirs, and makes microemulsion or aqueous emulsion.

Example of formulations 4,5% fluazinam bromothalonil microemulsion

Fluazinam 0.5%, bromothalonil 4.5%, toluene 8% (solvent), farming breast No. 500 8% (emulsifier), agricultural emulsifier NO．600s 5% (emulsifier), farming breast No. 1,600 5% (emulsifier), sldium lauryl sulfate 2% (wetting agent), ethylene glycol 5% (antifreezing agent), water are supplemented to 100%

Example of formulations 5,10% fluazinam bromothalonil microemulsion

Fluazinam 9%, bromothalonil 1%, toluene 8% (solvent), farming breast No. 500 8% (emulsifier), agricultural emulsifier NO．600s 5% (emulsifier), farming breast No. 1,600 5% (emulsifier), sldium lauryl sulfate 2% (wetting agent), urea 5% (antifreezing agent), water are supplemented to 100%

Example of formulations 6,25% fluazinam bromothalonil aqueous emulsion

Fluazinam 5%, bromothalonil 20%, DMF (solvent) 5%, cyclohexanol (solvent) 10%, glycerine (antifreezing agent) 5%, phenethyl phenol polyethenoxy ether (emulsifier) 10%, calcium dodecyl benzene sulfonate (wetting agent) 5%, water are supplemented to 100%

Example of formulations 7,30% fluazinam bromothalonil aqueous emulsion

Fluazinam 20%, bromothalonil 10%, DMF (solvent) 5%, cyclohexanol (solvent) 10%, glycerine (antifreezing agent) 5%, phenethyl phenol polyethenoxy ether (emulsifier) 10%, withered (wetting agent) 5% of tea, water are supplemented to 100%

Suspending agent embodiment

The ratio of each component such as active component, dispersant, defoamer, thickener and water in prescription mixed, after sand milling or high speed shear, obtain semi-finished product, add water after the analysis and mix filtration and namely get suspending agent.

Example of formulations 8,40% fluazinam bromothalonil suspending agent

Fluazinam 35%, prochloraz-manganese chloride complex 5%, polyoxyethylene nonylphenol ether 8% (emulsifier), propane diols 2% (cosolvent), xanthans 1% (thickener), organosilicon 2% (defoamer), water are supplemented to 100%

Example of formulations 9,35% fluazinam bromothalonil suspending agent

Fluazinam 5%, bromothalonil 30%, polyoxyethylene nonylphenol ether 8% (emulsifier), propane diols 2% (cosolvent), methylcellulose 1% (thickener), organosilicon 2% (defoamer), water are supplemented to 100%

Example of formulations 10,20% fluazinam bromothalonil suspending agent

Fluazinam 15%, bromothalonil 5%, polyoxyethylene nonylphenol ether 8% (emulsifier), propane diols 2% (cosolvent), CMC 1% (thickener), organosilicon 2% (defoamer), water are supplemented to 100%

Water dispersible granules embodiment

Pesticide activity component, auxiliary agent and filler are mixed in proportion, through air-flow crushing, then add the water of 15-20%, make the water dispersible granules product through kneading, granulation, drying, screening.Capital equipment: mixer, airslide disintegrating mill, kneader, Squeezinggranulator, drying equipment (drying room or fluid bed).

Example of formulations 11,80% fluazinam bromothalonil water dispersible granules

Fluazinam 50%, bromothalonil 30%, neopelex 4% (wetting agent), calcium lignosulfonate 10% (dispersant), ammonium sulfate 3% (disintegrant), kaolin (filler) are to 100%

Example of formulations 12,50% fluazinam bromothalonil water dispersible granules

Press fluazinam 25%, bromothalonil 25%, spaonin powder 4% (wetting agent), polycarboxylate 10% (dispersant), urea 3% (disintegrant), bentonite (filler) to 100%

Wetting powder embodiment

Pesticide activity component, auxiliary agent, filler mixed behind air-flow crushing to mix in proportion again make wetting powder.Capital equipment: mixer, airslide disintegrating mill.

Example of formulations 13,70% fluazinam bromothalonil wetting powder

Press fluazinam 20%, bromothalonil 50%, bay alcohol radical sodium sulphate 4% (wetting agent), mersolates 7% (dispersant), white carbon 8% (filler), kaolin (filler) to 100%

Example of formulations 14,50% fluazinam bromothalonil wetting powder

Fluazinam 40%, bromothalonil 10%, spaonin powder 4% (wetting agent), calcium lignosulfonate 7% (dispersant), white carbon 8% (filler), bentonite (filler) is to 100%,

Example of formulations 15,60% fluazinam bromothalonil wetting powder

Fluazinam 50%, bromothalonil 10%, pull open powder 4% (wetting agent), polycarboxylate 7% (dispersant), white carbon 8% (filler), precipitated calcium carbonate (filler) to 100%,

The field Application Example

Field Application Example 1 composition for preventing and controlling citrus anthracnose field trial

Test is processed and consumption sees Table 2.Repeat 4 residential quarters of every processing, and random district group is arranged 3 mandarin trees in every residential quarter.Every residential quarter medication 15L, medication was 1 time in per 7 days, shared medicine 2 times.

Investigation method: 7 days investigation disease situations before the dispenser, after the 2nd medication.2 strains are looked in every residential quarter during investigation, every strain by East, West, South, North, in 5 orientation get 3 branches, each branch is investigated whole blades, classification record occurring degree.Calculate disease index and relative control effect.

Grade scale is as follows: 0 grade, without scab; 1 grade, lesion area accounts for below 10% of whole leaf area; 3 grades, lesion area accounts for the 11%-25% of whole leaf area; 5 grades, lesion area accounts for the 26%-40% of whole leaf area; 7 grades, lesion area accounts for the 41%-65% of whole leaf area; 9 grades, lesion area accounts for more than 65% of whole leaf area.Disease refers to and the preventive effect computing formula:

As can be seen from Table 2: fluazinam and bromothalonil single dose are respectively 81.02%, 79.08% to the preventive effect of citrus anthracnose, and embodiment of the invention bactericidal composition all is significantly increased on preventive effect, and minimum preventive effect is 89.77%, has been up to 93.40%.The result of field trial fully shows, fluazinam and bromothalonil compounded have synergistic function after to citrus anthracnose, in the situation that effective ingredient consumption reduces, control efficiency significantly improves.Therefore, the present composition has the beneficial effect that reduces cost, delaying drug resistance, minimizing spraying times and residue of pesticide.

Table 2 fluazinam and bromothalonil compounded are to the field test results of citrus anthracnose

Reagent agent	Dosage (ga.i./ha)	Disease index before the medicine	7 days disease indexs behind the 2nd medicine	Preventive effect (%)
					500 grams per liter fluazinam suspending agents	250	4.2	14.5	81.02
25% bromothalonil wetting powder	375	4.6	17.5	79.08
					Example of formulations 1	190	3.9	5.7	91.96
Example of formulations 2	240	4.1	5.3	92.89
					Example of formulations 4	220	3.5	5.8	90.89
Example of formulations 7	200	3.3	5.2	91.34
					Example of formulations 9	230	4.3	6.8	91.31
Example of formulations 12	210	3.6	6.7	89.77
					Example of formulations 14	200	4.5	5.4	93.40
CK	-	3.7	67.3	-

Field Application Example 2 composition for preventing and controlling citrus anthracnose field trials

Test is processed and consumption sees Table 3.The test kind is Luzon awns, the age of tree 5 years, and test is chosen in the mango early flowering season and carries out.Rainy before the medicine, be in mango anthracnose and fall ill optimum period.Middle fertility experimental field, managerial skills are good.Arrange experimental plot at random district's group, and 4 strains of every residential quarter are repeated in 4 residential quarters of every processing.No. 16 knapsack hand sprayers sprayings of workers and peasants are adopted in test, turn the ripe phase respectively at early flowering season, the phase of bearing fruit, middle fruit phase and fruit and respectively spray medicine once.7 days investigation results behind the last spray medicine, investigation method: random searching 3 strains of every residential quarter, every strain press East, West, South, North, in 5 samplings, 30 young fruits of every some investigation, the total fruit of record number, sick really number at different levels.

Sick fruit stage division:

0 grade: anosis;

1 grade: needle-like brown spot is less than 5 on every young fruit;

3 grades: needle-like brown spot is 6～15 on every young fruit;

5 grades: needle-like brown spot is 16～25 on every young fruit;

7 grades: needle-like brown spot is more than 26 on every young fruit, and spot links to each other.

9 grades: whole fruit is met personally scab, has red point-like thing to occur.

The preventive effect computational methods:

As can be seen from Table 3: fluazinam and bromothalonil single dose are respectively 77.6%, 75.3% to the preventive effect of mango anthracnose, and embodiment of the invention bactericidal composition all is significantly increased on preventive effect, and minimum preventive effect is 86.8%, has been up to 91.2%.The result of field trial fully shows, fluazinam and bromothalonil compounded have synergistic function after to mango anthracnose, in the situation that effective ingredient consumption reduces, control efficiency significantly improves.Therefore, the present composition has the beneficial effect that reduces cost, delaying drug resistance, minimizing spraying times and residue of pesticide.

Table 3 fluazinam and bromothalonil compounded are to the field test results of mango anthracnose

Reagent agent	Dosage (ga.i./ha)	7 days disease indexs behind the last medicine	Preventive effect (%)
				500 grams per liter fluazinam suspending agents	250	13.15	77.6
25% bromothalonil missible oil	375	14.49	75.3
				Example of formulations 1	190	5.69	90.3
Example of formulations 2	240	6.75	88.5
				Example of formulations 4	220	7.75	86.8
Example of formulations 7	200	5.16	91.2
				Example of formulations 9	230	6.16	89.5
Example of formulations 12	210	7.40	87.4
				Example of formulations 14	200	6.87	88.3
CK	-	58.7	-

Claims (5)

1. bactericidal composition, it is characterized in that: contain active ingredient fluazinam (A) and bromothalonil (B), the mass ratio of A and B is 1: 100～100: 1.

2. bactericidal composition according to claim 1, it is characterized in that: A and B mass ratio are 1: 40～40: 1.

3. bactericidal composition according to claim 1, it is characterized in that: A and B mass accumulation percentage composition are 5～80% in the composition.

4. according to claim 1 or 3 described bactericidal compositions, it is characterized in that: formulation is missible oil, microemulsion, aqueous emulsion, suspending agent, wetting powder, water dispersible granules.

5. the application of bactericidal composition according to claim 1 is characterized in that: bactericidal composition is used for control gourd, fruit and vegetable anthracnose.