CN101745531B - Application of photosynthetic bacteria fermentation broth in pesticide and metal combined contamination soil - Google Patents
Application of photosynthetic bacteria fermentation broth in pesticide and metal combined contamination soil Download PDFInfo
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- CN101745531B CN101745531B CN2010101024467A CN201010102446A CN101745531B CN 101745531 B CN101745531 B CN 101745531B CN 2010101024467 A CN2010101024467 A CN 2010101024467A CN 201010102446 A CN201010102446 A CN 201010102446A CN 101745531 B CN101745531 B CN 101745531B
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Abstract
The invention provides application of photosynthetic bacteria fermentation broth in pesticide and metal combined contamination soil. In the application, the photosynthetic bacteria fermentation broth with the number effective viable bacteria to be between 108 and 1,010 per millimeter is diluted to 10 to 150 times with water; the fermentation broth is uniformly sprayed to degrading bacteria treated soil in dosage of between 10 and 50kg per mu; the earth surface is turned over for about 20 cm after the spraying is completed to ensure that the broth and the soil are sufficiently mixed and the photosynthetic bacteria can degrade residues of carbamic acid ester pesticide in the soil in high efficiency; and simultaneously, the bioavailability of the metal can be reduced to repair the carbamic acid ester pesticide and metal combined contamination soil. The photosynthetic bacteria has wide raw material sources, low price and easily controlled growing condition, can use low molecular organic substances as photosynthetic electron receptor to perform photo heterotrophic growth under anaerobic illuminating condition, can use organic substances as breathing substrate to perform aerobic or heterotrophic growth under dark and aerobic conditions, can carry out the photosynthesis without releasing oxygen, and is suitable for large-scale industrial implementation.
Description
Technical field
The present invention relates to a kind of microorganism application technology, specifically relate to the application of a kind of photosynthetic bacteria zymotic fluid in carbamate chemicals for agriculture and metal combined contamination soil reparation.
Background technology
Soil is the material base that the mankind depend on for existence, is the important component part of ecological environment.In recent years, the pollution problem of soil agricultural chemicals and heavy metal more and more comes into one's own, and bioremediation technology also becomes the focus of soil pollution reparation research.Relevant heavy metal-polluted soil, microorganism Decompounding of Pesticide research existing more bibliographical information (Wang C L, et al.Appl Environ Microbiol, 2000,66; Bang W, et al.Biotechnol Lett, 2000,22; Liu Xianhua etc., Nankai University's journal (natural science edition), 2003,36 (4); Zhang De chants etc., life science, 2005,9 (3); Wu Jun etc., ACTA Scientiae Circumstantiae, 2004,24 (2); Jiang Jiandong etc., bioengineering journal, 2005,21 (6); Zhang Liping etc., University Of Shanxi's journal (natural science edition), 2005,28 (1); Open star etc., soil journal, 2006,43 (4); Wu Jun etc., spectroscopy and spectrum analysis, 2006,26 (9); Feng Xiubin, Agricultural University Of Hebei's Master's thesis, 2008; Peng Xiang, Shandong Agricultural University's Master's thesis, 2008; Li Baoqing, Agricultural University Of Hebei's Master's thesis, 2008; Master's Ouyang, Agricultural University Of South China's journal, 2008,29 (2); Goswami S, et al.Biodegradation, 2009,20).Research through decades, screened the microbe groups that is separated to many degradable agricultural chemicals or transforms heavy metal, and develop some degradable agricultural chemicals or transform microbial degradation microbial inoculum or biodegradation fertilizer, the microbial compound preparation etc. of heavy metal, wherein outstanding with the research of Agricultural University Of Nanjing, this school enrichment screening is separated to the bacterial strain of the BHC of degrading, the red agricultural chemicals of furans, and the residues of pesticides microbial degradation technical research and application project of finishing obtains national science and technology progress second prize in 2005.At present just in the ascendant to the research of heavy metal, degradation of pesticide microorganism, but most of work still can not make degradation bacteria move towards practical application from the laboratory fully.
Photosynthetic bacteria be a kind of be the energy with light, with CO
2Or organic matter is the microorganism that carbon source is nourished and generated, and it is wide to have raw material sources of cultivation, low price, growth conditions is controlled easily, and thalline can be recycling etc. advantage, on agricultural, obtained in recent years to use comparatively widely.The research of relevant photosynthetic bacteria degrading pesticide residues or removal heavy metal lead, cadmium also has some bibliographical informations (Li Le etc., agricultural environment science journal, 2006,25 (B09); Luoyuan China etc., Tianjin agricultural sciences, 2009,2; A kind of bacterium for degradating residual agricultural chemical and production method thereof, ZL 01108446.4; The application of the red ferment product of class ball in chemical residual degradation, ZL200410036024.9; Bai Hongjuan etc., ACTA Scientiae Circumstantiae, 2006,26 (11); Bai Hongjuan etc., Chinese safe and scientific journal, 2007,17 (1)), but it is studied still based on laboratory research, and is that agricultural chemicals separates research with metal, and the application technology of photosynthetic bacteria zymotic fluid biological restoration carbamate pesticide and metal combined contamination is not appeared in the newspapers.
Summary of the invention
The objective of the invention is at above-mentioned the deficiencies in the prior art, the application of a kind of photosynthetic bacteria zymotic fluid in carbamate chemicals for agriculture and metal combined contamination soil reparation is provided.
The concrete grammar of the application of photosynthetic bacteria zymotic fluid provided by the invention in carbamate chemicals for agriculture and metal combined contamination soil reparation is: will contain effective viable count is 10
8-10
10The photosynthetic bacteria zymotic fluid of individual/ml is converted water dilution 10-150 doubly, use amount with every mu of 10-50kg evenly sprays in degradation bacteria processing soil, spray and turn over about the 20cm of the face of land after finishing, make the abundant mixing of bacterium liquid and soil, make degrade efficiently carbamate chemicals residual in the soil of photosynthetic bacteria, reduce the bioavailability of metal simultaneously, in order to repair carbamate chemicals for agriculture and metal combined contamination soil.
Usually, can carry out flood irrigation to handling soil spraying preceding 2 days of photosynthetic bacteria zymotic fluid, to improve the moisture of soil.
Wherein, described soil is the soil of pH value for 5.0-9.0, and described metallic pollution is lead, cadmium, mercury, copper, zinc, nickel contamination.
Described photosynthetic bacteria can be any strain bacterium or their any combination in the following photosynthetic bacteria: Rhodospirillum Rhodospirillium, as Rhodospirillum rubrum (Rhodospirillium rubrum); Rhodopseudomonas Rhodopseudomonas is as Rhodopseudomonas palustris (Rhodopseudomonas palustris), Green color rhodopseudomonas (Rhodopseudomonas viridis), rhodopseudononas sulfovirdis (Rhodopseudomonas sulfoviridis), red marine pseudomonas (Rhodopseudomonasmarina); Red bacterium belongs to Rodobacter, as Spherical red antibacterial (Rhodobacter sphaeroides) etc.
The document source of above-mentioned bacterial classification: Yao Zhuyun, Zhang Zhaoming. phenotypic characteristic of a few strain photosynthetic bacterias and DNA-DNA homology analysis thereof. use and the environmental organism journal 1996,2 (1): 84-89; Zhang Zhaoming, Yang Suping, Zhao Chungui. the separation identification research of Rhodopseudomonas palustris. University Of Shanxi's journal (natural science edition), 1992, (4): 379-385; Yang Suping, Zhang Zhaoming, Zhao Chungui. separating and evaluation of Green color rhodopseudomonas and rhodopseudononas sulfovirdis. microorganism journal, 1995,35 (2): 91-96; Zhang Zhaoming, Deng Songlu, Zhao Liangqi etc. the research of separation, evaluation and the physiological property of the research A. Rhodopseudomonas spheroides of Purple Non-sulfur photosynthetic bacteria. University Of Shanxi's journal (natural science edition), 1984, (4): 54-59.
These bacterial classifications all come from Life Science and Technology institute of Shanxi province Taiyuan city University Of Shanxi photosynthetic bacteria research department.
The photosynthetic bacteria zymotic fluid that the present invention uses is to adopt the medium culture of following suitable photosynthetic bacterium growth to obtain:
Sodium acetate 1000-2000mg CaCl
22H
2O 50-100mg
MgSO
4·7H
2O 100-300mg EDTA 10-30mg
Yeast extract 500-1500mg K
2HPO
4500-1500mg
(NH
4)
2SO
4 1000-2000mg KH
2PO
4 400-1000mg
FeSO
47H
2O 5-15mg trace element solution 1-5ml
Deionized water 1000-2000ml
Wherein, consisting of of trace element solution:
H
3BO
3 200-300mg Na
2MoO
4·2H
2O 50-100mg
CuSO
4 2-10mg MnSO
4·4H
2O 150-250mg
ZnSO
47H
2O 20-30mg deionized water 100-200ml
The pH value of above culture medium is 5-9.
The present invention utilizes photosynthetic bacteria (PSB) microbial fermentation solution that carbamate chemicals for agriculture and metal combined contamination soil are repaired, the carbamate chemicals residual in the soil of can degrading efficiently, and can reduce the bioavailability of metal.
Photosynthetic bacteria can utilize low molecule organic matter to carry out the photoheterotrophy growth as photosynthetic electron acceptor under the anaerobism illumination condition; Under dark aerobic condition, can utilize organic matter to carry out aerobic or heterotrophic growth as respiratory substrate; Can not put the photosynthesis of oxygen.The cultivation raw material sources of photosynthetic bacteria are wide, low price, and growth conditions is controlled easily, suitability for mass industrialized enforcement, therefore, the technology of the present invention has broad application prospects.
The specific embodiment
Embodiment 1
Dispose culture medium by following component:
Sodium acetate 1500mg CaCl
22H
2O 80mg
MgSO
4·7H
2O 150mg EDTA 20mg
Yeast extract 1200mg K
2HPO
41000mg
(NH
4)
2SO
4 1500mg KH
2PO
4 800mg
FeSO
47H
2O 10mg trace element solution 3ml
Deionized water 1500ml
Consisting of of trace element solution: H wherein
3BO
3240mg; Na
2MoO
42H
2O 70mg; CuSO
47mg; MnSO
44H
2O 200mg; ZnSO
47H
2O 24mg; Deionized water 1650ml.
The medium pH value that is mixed with is 7.
At the Spherical red antibacterial that in the culture medium of sterilization, inserts 1/20 umber, under illumination 2500lux, room temperature, anaerobic condition, cultivated 20 days, obtain photosynthetic bacteria Spherical red antibacterial zymotic fluid.
At the one mu of red pollution concentration 15mg/kg of furans soil after testing, cadmium pollution concentration 25mg/kg soil in the soil of pH=5.0, carries out flood irrigation to handling soil spraying preceding 2 days of microbial inoculum, and containing effective viable count with 10kg is 3 * 10
9The Spherical red antibacterial zymotic fluid of individual/ml is converted 50 times water and is sprayed in handling soil, spraying the back that finishes and adopt machinery to turn over about the 20cm of the face of land, makes the abundant mixing of bacterium liquid and soil.Sampling in the 15th day detects, and the degradation rate of furans pellet is 90%, Cd
2+Bioavailability reduce by 60%.
Embodiment 2
Use embodiment 1 culture medium and cultural method to cultivate and obtain photosynthetic bacteria Rhodopseudomonas palustris zymotic fluid.
At one mu of Aldicarb pollution concentration 10mg/kg soil after testing, concentration of lead contamination 500mg/kg soil in the soil of pH=8.5, sprays preceding 2 days of microbial inoculum and carries out flood irrigation to handling soil, and containing effective viable count with 30kg is 5 * 10
8The Rhodopseudomonas palustris zymotic fluid of individual/ml is converted 80 times water and is sprayed in handling soil, spraying the back that finishes and adopt machinery to turn over about the 20cm of the face of land, makes the abundant mixing of bacterium liquid and soil.Sampling in the 15th day detects, and the degradation rate of Aldicarb is 94%, Pb in the soil
2+Bioavailability reduce by 70%.
Embodiment 3
Use embodiment 1 culture medium and cultural method to cultivate the mixed fermentation liquid that obtains photosynthetic bacteria Green color rhodopseudomonas and red marine pseudomonas.
At one mu of chlorpropham pollution concentration 30mg/kg soil after testing, copper pollution concentration 600mg/kg soil in the soil of pH=6.5, sprays preceding 2 days of microbial inoculum and carries out flood irrigation to handling soil, and containing effective viable count with 15kg is 8 * 10
9The mixed fermentation liquid of the Green color rhodopseudomonas of individual/ml and red marine pseudomonas is converted 100 times water and is sprayed in handling soil, spraying the back that finishes and adopt machinery to turn over about the 20cm of the face of land, makes the abundant mixing of bacterium liquid and soil.Sampling in the 15th day detects, and the degradation rate of chlorpropham is 93%, Cu in the soil
2+Bioavailability reduce by 75%.
Embodiment 4
Use embodiment 1 culture medium and cultural method to cultivate the mixed fermentation liquid that obtains photosynthetic bacteria Rhodospirillum rubrum and red pseudomonas.
At the one mu of red pollution concentration 40mg/kg of furans soil after testing, zinc and mercury pollution concentration are respectively 600mg/kg soil and 10mg/kg soil, in the soil of pH=7.0, spray preceding 2 days of microbial inoculum and carry out flood irrigation to handling soil, and containing total living bacteria count with 45kg is 6 * 10
8The mixed fermentation liquid of the Rhodospirillum rubrum of individual/ml and red pseudomonas is converted 150 times water and is sprayed in handling soil, spray finish after, adopt machinery to turn over about the 20cm of the face of land, make the abundant mixing of bacterium liquid and soil.Sampling in the 15th day detects, and the degradation rate of furans pellet is 84%, Zn in the soil
2+And Hg
2+Bioavailability reduce by 76% and 70% respectively.
Above embodiment is only for the present invention is further illustrated, and scope of the present invention is not subjected to the limitation of illustrated embodiment.
Claims (3)
1. the application of photosynthetic bacteria zymotic fluid in carbamate chemicals for agriculture and metal combined contamination soil reparation is characterized in that with containing effective viable count be 10
8-10
10The photosynthetic bacteria zymotic fluid of individual/ml and water are with 1: 10-1: 150 volume ratio is mixed with the aqueous solution, and evenly spray in the pH value according to every mu of 10～50kg and handle soil for the degradation bacteria of 5.0-9.0,
Wherein, described photosynthetic bacteria is any strain bacterium during following photosynthetic bacteria belongs to or their any hybrid bacterial strain: Rhodospirillum Rhodospirillium, and Rhodopseudomonas Rhodopseudomonas, red bacterium belongs to Rodobacter.
2. the application of photosynthetic bacteria zymotic fluid according to claim 1 is characterized in that carrying out flood irrigation to handling soil spraying preceding 2 days of photosynthetic bacteria zymotic fluid.
3. the application of photosynthetic bacteria zymotic fluid according to claim 1 is characterized in that described metallic pollution is lead, cadmium, mercury, copper, zinc, nickel contamination.
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| CN102224786B (en) * | 2011-04-28 | 2012-07-25 | 山西医科大学 | Method and application of photosynthetic bacteria culture solution in medicinal material planting |
| CN102240670B (en) * | 2011-05-12 | 2012-12-19 | 北京航空航天大学 | Method for repairing microorganisms in heavy metal contaminated soil |
| CN103114039A (en) * | 2013-02-22 | 2013-05-22 | 广州天壤生物科技有限公司 | Biological agent capable of repairing heavy metal contaminated soil and soil remediation method |
| CN103320350B (en) * | 2013-05-13 | 2015-03-04 | 中国科学院苏州纳米技术与纳米仿生研究所 | Tistrella mobilis bacterial strain, polycyclic aromatic hydrocarbon degradation reagent, soil remediation reagent, and the application of Tistrella mobilis bacterial strain |
| CN103691737B (en) * | 2014-01-09 | 2015-04-08 | 江苏麦可博生物环保工程技术有限公司 | Combined process for ex-situ remediation of degradation-resistant organic contaminated soil by microorganisms |
| CN104707864B (en) * | 2015-03-04 | 2017-05-24 | 北京聚益成广科技有限公司 | Compound photosynthetic bacteria preparation for enhancing phytoremediation for heavy metal pollution of soil and preparation method thereof |
| CN105123013B (en) * | 2015-09-29 | 2017-03-08 | 河南寰宇环保科技有限公司 | Gold tailing ponds surface cleaning and the method for restoration of the ecosystem |
| CN107475155A (en) * | 2017-09-15 | 2017-12-15 | 黑龙江宝基农业科技股份有限公司 | The soil remediation composite bacteria agent and its soil remediation method for soil fertilizer medicine residual of degrading |
| CN109127699B (en) * | 2018-08-31 | 2020-02-14 | 成都理工大学 | Soil remediation device utilizing solar energy and using method |
| CN109257993A (en) * | 2018-09-20 | 2019-01-25 | 郭锦雄 | The field cultivation method of microorganism efficient degradation soil pesticide residues and curing heavy metal |
| CN110355196A (en) * | 2019-07-12 | 2019-10-22 | 重庆丰衣谷农业科技有限公司 | A kind of microbe soil restoration agent and its rehabilitating soil method |
| CN111733116B (en) * | 2020-08-14 | 2023-02-03 | 中北大学 | Photosynthetic bacterium composite microorganism mixed culture microbial inoculum and method for repairing chromium-contaminated soil |
| CN112474781B (en) * | 2020-11-23 | 2021-09-17 | 纳琦绿能工程有限公司 | Composite material for repairing heavy metal contaminated soil and preparation method and application thereof |
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Application publication date: 20100623 Assignee: Guangzhou Tianrang Biological Science & Technology Co., Ltd. Assignor: Zhongbei Univ Contract record no.: 2014440000324 Denomination of invention: Application of photosynthetic bacteria fermentation broth in pesticide and metal combined contamination soil Granted publication date: 20110831 License type: Exclusive License Record date: 20140704 |
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