CN101744649A - System for transferring anti-AIDS vaccine - Google Patents
System for transferring anti-AIDS vaccine Download PDFInfo
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- CN101744649A CN101744649A CN200810204637A CN200810204637A CN101744649A CN 101744649 A CN101744649 A CN 101744649A CN 200810204637 A CN200810204637 A CN 200810204637A CN 200810204637 A CN200810204637 A CN 200810204637A CN 101744649 A CN101744649 A CN 101744649A
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Abstract
The invention discloses a system for transferring an anti-AIDS vaccine, which can make the anti-AIDS DNA vaccine enter cells so as to improve the efficiency of transfer of the vaccine to the human body and enhance the treating effect. The invention adopts a technical scheme that: the system comprises an injection device and an electroporation transfer device, wherein the injection device stores the anti-AIDS vaccine; and the electroporation transfer device comprises a pulse setting module, a power supply module, a micro controller, a pulse generating module and a load; the pulse setting module receives an electric pulse parameter inputted by a user; the power supply module generates the DC electric signal; the micro controller receives the electric pulse parameters of the pulse setting module and outputs the control signal according to the electric pulse parameters; the pulse generating module receives the control signal outputted by the micro controller, processes the DC electric signal sent by the power supply module into the electric pulse signal and outputs the electric pulse signal, the waveform of the outputted electric pulse is the symmetrical triggering unilaterally continuous pulse wave, the pulse frequency is 1 to 50Hz, the pulse width is 9.5 to 50ms, the number of pulses is 1 to 20, and the pulse interval is 1 to 100ms; and the load receives the electric pulse signal outputted by the pulse generating module. The system can be applied in the vaccine transferring field.
Description
Technical field
The present invention relates to a kind of vaccine import system, relate in particular to a kind of system that utilizes electroporation technology anti-AIDS vaccine to be imported target tissue.
Background technology
As far back as 1981, just found the first in the world example aids patient, virus constantly makes a variation, gene has multiformity, vaccine development is with high costs causes the vaccine development of acquired immune deficiency syndrome (AIDS) to be affected with application.Improve effective percentage, reducing cost becomes main focus.Vaccine divides vaccine and treatment vaccine, the effect of vaccine is the virus of protecting from infection, and the effect of treatment vaccine then is to lure that the human immune system brings into play the damage of opposing virus to human body into, perhaps the enhance immunity system is to the resistivity of virus, at be the crowd of infective virus.From 1985 before and after the nineteen ninety, the quantity of AIDSinfected patient is in build phase at a slow speed, to the later stage nineties 20th century, the quantity of AIDSinfected patient enters the stage of quick growth.At present, the quantity of HIV (human immunodeficiency virus) infection has presented rapid growth, logarithmic growth period.Not only to prevent, and will be in the face of more HIV the infecteds' treatment.The topmost problem of HIV sufferers is the forfeiture of immunologic function, as long as find a kind of method that can effectively trigger and recover himself immunocompetence so, the state of an illness should be controlled.
But the importing of anti-AIDS vaccine is low at present, has objectively influenced the performance of its effect.So will give full play to the function of dna vaccination, also must improve it and import efficient to reach the effect that improves its immune performance.
Summary of the invention
The objective of the invention is to address the above problem, a kind of system that imports anti-AIDS vaccine is provided, can make the dna vaccination of anti-AIDS enter cell, import intravital efficient, strengthen therapeutic effect thereby improve it.
Technical scheme of the present invention is: the present invention has disclosed a kind of system that imports anti-AIDS vaccine, and anti-AIDS vaccine is imported target tissue by low-frequency electric pulse, and this system comprises:
Injection device contains anti-AIDS vaccine;
Electroporation leading-in apparatus comprises:
Pulse is provided with module, receives the electric pulse parameter of user's input;
Power module produces a direct current signal of telecommunication;
Microcontroller receives the electric pulse parameter that this pulse is provided with module, exports a control signal according to this electric pulse parameter;
The pulse generation module, receive the control signal of this microcontroller output, the dc signal that this power module is supplied with is processed into electric impulse signal and output, the waveform of the electric pulse of output is that symmetry triggers unidirectional continuous impulse ripple, pulse frequency is 1～50Hz, pulse width is 9.5ms～50ms, and pulse number is 1～20, and the pulse spacing is 1～100ms;
Load receives the electric impulse signal that this pulse generation module is exported.
The system of above-mentioned importing anti-AIDS vaccine, wherein, the pulse number that once discharges of this electric pulse is 6, and the error rate of pulse frequency is ± 15%, and the error rate of pulse width is ± and 30%.
The system of above-mentioned importing anti-AIDS vaccine, wherein, this load is the acupuncture needle electrode, be formed by connecting by electrode tip and electrode handle, wherein the plug of this electrode tip is inserted in the groove of this electrode handle, and the maximum of this acupuncture needle electrode output crest voltage is 36V～180V, and error rate is ± 10%.
The system of above-mentioned importing anti-AIDS vaccine, wherein, this electroporation leading-in apparatus also comprises:
The detection module that comes off connects this pulse generation module, and the electric impulse signal in this load is sampled, and sampled signal is sent into this microcontroller judge.
The system of above-mentioned importing anti-AIDS vaccine, wherein, this electroporation leading-in apparatus also comprises:
The pulse display module receives the electric pulse parameter in this microcontroller, is shown to the user on a display screen.
The system of above-mentioned importing anti-AIDS vaccine, wherein, this power module of this electroporation leading-in apparatus is a dc source.
The system of above-mentioned importing anti-AIDS vaccine, wherein, this power module of this electroporation leading-in apparatus comprises:
Alternating current power supply;
Toroidal transformer, primary coil connect this alternating current power supply, and secondary coil has a plurality of taps and each tap all to be connected with a current rectifying and wave filtering circuit, and after the voltage process transformation with alternating current power supply, voltage stabilizing becomes DC voltage output again.
The system of above-mentioned importing anti-AIDS vaccine, wherein, this pulse generation module of this electroporation leading-in apparatus is the half-bridge amplifying circuit.
The system of above-mentioned importing anti-AIDS vaccine, wherein, this pulse generation module of this electroporation leading-in apparatus is Zhan Boqi.
The system of above-mentioned importing anti-AIDS vaccine, wherein, this of this electroporation leading-in apparatus testing circuit that comes off comprises:
The diode of two serial connections, first end connect this pulse generation module, the second end ground connection;
A transistor, base stage connect first end of the diode of these two serial connections, and colelctor electrode connects power end, grounded emitter and be connected by resistance with base stage.
The system of above-mentioned importing anti-AIDS vaccine, wherein, this microcontroller of this electroporation leading-in apparatus comprises:
Pulse is provided with judging unit, if do not receive the electric pulse parameter that sets, then need notify the user that electric pulse parameter is set;
Start judging unit, move the electric pulse generative process if receive the start-up control signal then start, otherwise keep current state constant;
The control signal generation unit produces the pwm signal that is used to generate corresponding electric pulse according to the electric pulse parameter that sets;
The detection judging unit that comes off judges whether load comes off according to the having or not of sampled signal of this detection module that comes off.
The present invention contrasts prior art, following beneficial effect is arranged: the present invention injects anti-AIDS vaccine by utilizing injection device earlier, send the target tissues such as muscle that low-frequency electric pulse imports stimulates human body by electroporation leading-in apparatus then, make in the dna vaccination molecule transfered cell of anti-AIDS, strengthened the effect of treatment.And can adjust the importing effect of anti-AIDS vaccine by the design of waveform parameter, reach the effect of efficient, low amount ground opposing acquired immune deficiency syndrome (AIDS).
Description of drawings
Fig. 1 is the schematic diagram of preferred embodiment of the system of importing anti-AIDS vaccine of the present invention.
Fig. 2 is the structural representation of the preferred embodiment of load of the present invention.
Fig. 3 A is the schematic diagram of a preferred embodiment of power module of the present invention.
Fig. 3 B is the circuit diagram of Fig. 3 A embodiment.
Fig. 4 is the schematic diagram of microprocessor of the present invention.
Fig. 5 is the circuit diagram of a preferred embodiment of half-bridge amplifying circuit of the present invention.
Fig. 6 is the circuit diagram of a preferred embodiment of the testing circuit that comes off of the present invention.
Fig. 7 is the oscillogram of electroporation leading-in apparatus output of the present invention.
The specific embodiment
The invention will be further described below in conjunction with drawings and Examples.
Fig. 1 shows the principle of a preferred embodiment of the system of importing anti-AIDS vaccine of the present invention, sees also Fig. 1.The system of present embodiment comprises electroporation leading-in apparatus 1 and injection device 2 two parts.Wherein injection device 2 can be a needle cylinder injection device.Electroporation leading-in apparatus 1 comprises: pulse is provided with module 10, power module 20, microcontroller 30, pulse display module 40, pulse generation module 50, load 60 and the detection module 70 that comes off.
Pulse is provided with the electric pulse parameter that module 10 receives user's input.The scope that electric pulse parameter should be provided with is: the waveform of electric pulse is for triggering unidirectional continuous impulse ripple to one-tenth, pulse frequency is 1～50Hz, and electric pulse is for once discharging 1～20 pulse, and preferable is 6, pulse width is 9.5ms～50ms, and the pulse spacing is 1～100ms.The error rate of above-mentioned pulse frequency is ± 15%, the error rate of pulse width is ± and 30%.
Power module 20 produces a direct current signal and exports pulse generation module 50 to as driving voltage, and power module 20 provides running voltage to microprocessor 30 simultaneously.Power module 20 can be a continuous-current plant, also can be an ac power supply apparatus.Please be simultaneously referring to Fig. 3 A and 3B, when power module 20 is an ac power supply apparatus, the alternating current power supply 21 of 220V is connected on the primary coil of toroidal transformer 22, the secondary coil of transformator has 3 taps, transformation through toroidal transformer 22, in the 1st tap, form the alternating voltage of 36V, in the 2nd tap, form the alternating voltage of 60V, in the 3rd tap, form the alternating voltage of 5V.Three taps connect a current rectifying and wave filtering circuit 23 respectively, respectively the alternating current of 36V are converted to the unidirectional current of 36V, and the alternating current of 60V is converted to the unidirectional current of 60V, the alternating current of 5V are converted to the unidirectional current of 5V.Wherein 36V unidirectional current and 60V unidirectional current are as the driving voltage of exporting to pulse generation module 50, and the 5V unidirectional current is exported to the running voltage of microcontroller 30 as microcontroller.
Pulse generation module 50 receives the control signal of microcontrollers 30 outputs, exports after the direct current signal that power module 20 is supplied with is processed into pulse signal.The specific implementation of pulse generation module 50 can be Zhan Boqi, also can be half-bridge amplifying circuit or full-bridge amplifying circuit.Please be simultaneously referring to Fig. 5, Fig. 5 shows the circuit structure of half-bridge amplifying circuit.The pwm control signal of microcontroller 30 outputs through the RC circuit filtering, amplifies pwm signal by transistor Q1, Q3, respectively driving transistors Q2, Q4.Transistor Q2 and Q4 constitute half-bridge circuit, and last pulse signal is exported from terminal A, B.The electric impulse signal that pulse generation module 50 produces is provided with module 10 by pulse and is provided with, the waveform of the electric pulse of being exported as shown in Figure 7, be that symmetry triggers unidirectional continuous impulse ripple, pulse frequency is 1～50Hz (error rate is ± 15%), pulse width is 9.5ms～50ms (error rate is ± 30%), electric pulse once discharges 1～20 pulse, and preferable is 6, and the pulse spacing is 1～100ms.
The detection module 70 that comes off connects pulse generation module 50, and the electric impulse signal in the load is sampled, and sampled signal is sent in the microcontroller 30 judged.Fig. 6 shows the physical circuit of the detection module that comes off, please be simultaneously referring to Fig. 5.Come off in the detection module 70, diode D3, D4 connect, and an end connects the colelctor electrode of transistor Q4 in the half-bridge circuit, other end ground connection.The power end of the colelctor electrode of transistor Q5 by resistance R 9 connections+5V, base stage connects the end of diode D3 by resistance R 7, grounded emitter and pass through resistance R 8 and link to each other with base stage.If load electrode does not come off, then diode D3, D4 sample to the pwm pulse signal in the load, and the amplifying circuit that constitutes by transistor Q5 is amplified sampled signal, and the input port of sending into microcontroller 30 is again handled and judged.If load electrode comes off, then come off between testing circuit and the load electrode to form and open circuit, diode D3, D4 detect less than pwm pulse signal, also just do not have sampled signal to send into the input port of microcontroller 30, and microcontroller 30 is judged as load and comes off at this moment.
An embodiment of load 60 is the acupuncture needle electrode as shown in Figure 2, be formed by connecting by electrode tip 601 and electrode handle 602, the plug of electrode tip 601 inserts in the groove of electrode handle 602, and the maximum of acupuncture needle electrode output crest voltage is 36V～180V, and error rate is ± 10%.The acupuncture needle electrode is inserted in the injection site of syringe before this, finishes the process of anti-AIDS vaccine transfered cell with above-mentioned electric pulse.
The foregoing description provides to those of ordinary skills and realizes or use of the present invention; those of ordinary skills can be under the situation that does not break away from invention thought of the present invention; the foregoing description is made various modifications or variation; thereby protection scope of the present invention do not limit by the foregoing description, and should be the maximum magnitude that meets the inventive features that claims mention.
Claims (11)
1. a system that imports anti-AIDS vaccine imports target tissue with anti-AIDS vaccine by low-frequency electric pulse, and this system comprises:
Injection device contains anti-AIDS vaccine;
Electroporation leading-in apparatus comprises:
Pulse is provided with module, receives the electric pulse parameter of user's input;
Power module produces a direct current signal of telecommunication;
Microcontroller receives the electric pulse parameter that this pulse is provided with module, exports a control signal according to this electric pulse parameter;
The pulse generation module, receive the control signal of this microcontroller output, the dc signal that this power module is supplied with is processed into electric impulse signal and output, the waveform of the electric pulse of output is that symmetry triggers unidirectional continuous impulse ripple, pulse frequency is 1～50Hz, pulse width is 9.5ms～50ms, and pulse number is 1～20, and the pulse spacing is 1～100ms;
Load receives the electric impulse signal that this pulse generation module is exported.
2. the system of importing anti-AIDS vaccine according to claim 1 is characterized in that, the pulse number that once discharges of this electric pulse is 6, and the error rate of pulse frequency is ± 15%, the error rate of pulse width is ± and 30%.
3. the system of importing anti-AIDS vaccine according to claim 2, it is characterized in that, this load is the acupuncture needle electrode, be formed by connecting by electrode tip and electrode handle, wherein the plug of this electrode tip is inserted in the groove of this electrode handle, the maximum output crest voltage of this acupuncture needle electrode is 36V～180V, and error rate is ± 10%.
4. according to the system of each described importing anti-AIDS vaccine in the claim 1～3, it is characterized in that this electroporation leading-in apparatus also comprises:
The detection module that comes off connects this pulse generation module, and the electric impulse signal in this load is sampled, and sampled signal is sent into this microcontroller judge.
5. according to the system of each described importing anti-AIDS vaccine in the claim 1～3, it is characterized in that this electroporation leading-in apparatus also comprises:
The pulse display module receives the electric pulse parameter in this microcontroller, is shown to the user on a display screen.
6. according to the system of each described importing anti-AIDS vaccine in the claim 1～3, it is characterized in that this power module of this electroporation leading-in apparatus is a dc source.
7. according to the system of each described importing anti-AIDS vaccine in the claim 1～3, it is characterized in that this power module of this electroporation leading-in apparatus comprises:
Alternating current power supply;
Toroidal transformer, primary coil connect this alternating current power supply, and secondary coil has a plurality of taps and each tap all to be connected with a current rectifying and wave filtering circuit, and after the voltage process transformation with alternating current power supply, voltage stabilizing becomes DC voltage output again.
8. according to the system of each described importing anti-AIDS vaccine in the claim 1～3, it is characterized in that this pulse generation module of this electroporation leading-in apparatus is the half-bridge amplifying circuit.
9. according to the system of each described importing anti-AIDS vaccine in the claim 1～3, it is characterized in that this pulse generation module of this electroporation leading-in apparatus is Zhan Boqi.
10. according to the system of each described importing anti-AIDS vaccine in the claim 1～3, it is characterized in that this of this electroporation leading-in apparatus testing circuit that comes off comprises:
The diode of two serial connections, first end connect this pulse generation module, the second end ground connection;
A transistor, base stage connect first end of the diode of these two serial connections, and colelctor electrode connects power end, grounded emitter and be connected by resistance with base stage.
11. the system of importing anti-AIDS vaccine according to claim 4 is characterized in that, this microcontroller of this electroporation leading-in apparatus comprises:
Pulse is provided with judging unit, if do not receive the electric pulse parameter that sets, then need notify the user that electric pulse parameter is set;
Start judging unit, move the electric pulse generative process if receive the start-up control signal then start, otherwise keep current state constant;
The control signal generation unit produces the pwm signal that is used to generate corresponding electric pulse according to the electric pulse parameter that sets;
The detection judging unit that comes off judges whether load comes off according to the having or not of sampled signal of this detection module that comes off.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810204637A CN101744649A (en) | 2008-12-16 | 2008-12-16 | System for transferring anti-AIDS vaccine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810204637A CN101744649A (en) | 2008-12-16 | 2008-12-16 | System for transferring anti-AIDS vaccine |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102512756A (en) * | 2011-12-06 | 2012-06-27 | 何宗彦 | Method and equipment for generating high-voltage pulse induction currents in object |
| CN103340698A (en) * | 2013-07-23 | 2013-10-09 | 成都安铂奥金生物科技有限公司 | Inoculation method and inoculation device of gene vaccine |
| CN106924874A (en) * | 2015-12-31 | 2017-07-07 | 上海塔瑞莎生物技术有限公司 | The system that a kind of constant pressure electric pulse imports AIDS nucleic acid vaccine |
| CN113274116A (en) * | 2021-05-12 | 2021-08-20 | 香港普创生物科技有限公司 | Full-automatic electric pulse perforation syringe |
-
2008
- 2008-12-16 CN CN200810204637A patent/CN101744649A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102512756A (en) * | 2011-12-06 | 2012-06-27 | 何宗彦 | Method and equipment for generating high-voltage pulse induction currents in object |
| CN102512756B (en) * | 2011-12-06 | 2014-10-22 | 何宗彦 | Method and equipment for processing biological cell in object |
| CN103340698A (en) * | 2013-07-23 | 2013-10-09 | 成都安铂奥金生物科技有限公司 | Inoculation method and inoculation device of gene vaccine |
| CN103340698B (en) * | 2013-07-23 | 2016-09-07 | 成都安铂奥金生物科技有限公司 | The inoculation method of a kind of genetic immunization seedling and classification inoculation apparatus |
| CN106924874A (en) * | 2015-12-31 | 2017-07-07 | 上海塔瑞莎生物技术有限公司 | The system that a kind of constant pressure electric pulse imports AIDS nucleic acid vaccine |
| CN113274116A (en) * | 2021-05-12 | 2021-08-20 | 香港普创生物科技有限公司 | Full-automatic electric pulse perforation syringe |
| CN113274116B (en) * | 2021-05-12 | 2024-05-14 | 普亚生物科技有限公司 | Full-automatic electroporation syringe |
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Application publication date: 20100623 |