CN101732257B - Chitosan nanoparticle and preparation method and application thereof - Google Patents
Chitosan nanoparticle and preparation method and application thereof Download PDFInfo
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- CN101732257B CN101732257B CN2009102143140A CN200910214314A CN101732257B CN 101732257 B CN101732257 B CN 101732257B CN 2009102143140 A CN2009102143140 A CN 2009102143140A CN 200910214314 A CN200910214314 A CN 200910214314A CN 101732257 B CN101732257 B CN 101732257B
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Abstract
The invention discloses chitosan nanoparticle and a preparation method and application thereof. Chitosan and sodium tripolyphosphate are used to react and obtain the nanoparticle through rotary evaporation, concentration and spray drying. The invention adopts the simple and feasible method for the chitosan nanocrystallization, thus significantly increasing the activity of chitosan, solving the problem that chitosan is difficult to absorb and dissolve, greatly reducing the side effect and fundamentally resolving the current technical problems and technical bottleneck for losing weight and reducing blood fat of chitosan. The method of the invention is simple and feasible, has low cost and is easy to promote.
Description
Technical field
The invention belongs to medicine and functional food technical field, be specifically related to a kind of chitosan nano and its production and application.
Background technology
Chitosan (chitosan) is the N-deacetylation product of chitin, chemistry poly-(1,4)-2-amino by name-2-deoxidation
-D-glucosan.Extensively be present in unicellular lower eukaryote mushroom, alga cells, joint props up in the shell of animal shrimp, Eriocheir sinensis, insecticide, is to be only second to cellulosic second largest biological polyoses on the earth.Chitosan is a kind of natural, nontoxic, biodegradable chemical compound, and good biocompatibility, antibiotic property and adsorptivity are arranged, and fat and cholesterol are had the good adsorption performance.
Contain a plurality of amino in the chitosan molecule structure, as polycation, can with cholic acid, the cholesterol formation complex of combining closely of band anionic group in the animal body, such coordination compound is difficult for by the gastric acid hydrolysis, be difficult for being digested by digestive system, stop mammal to the digesting and assimilating of this class material, impelled it to excrete.Chitosan also can stop the absorption of digestive system to cholesterol, triglyceride simultaneously, promotes its catabolism, thereby produces effect for reducing blood fat, reduces cholesterol level and fat level in serum, the liver organization.
These good physiologically actives make chitosan begin to be applied to become a kind of novel blood fat reducing slimming medicine and functional food in the prevention and treatment of obesity and complication thereof.
But chitosan is applied to reducing weight and blood fat and also exists a lot of problems.Using chitosan its dosage in the reducing weight and blood fat experiment of human body generally is 2～3g/d, and fat-reducing effect is not obvious under this dosage, if but increased dosage amount is easy to generate constipation and the nauseating ill symptoms that waits, must increase the financial burden of user simultaneously.
In addition, the chitosan crystallinity is stronger, and solubility property is very poor, and the chemical property torpescence only can be dissolved in rare acid solution, has limited the application of chitosan in each field greatly.
Summary of the invention
The present invention seeks to deficiency, a kind of chitosan nano is provided, comprise chitosan nano and water-soluble chitosan nanoparticle at existing chitosan.Specifically the dosage that need use at existing chitosan cause untoward reaction greatly and easily, defective such as the overlong time that dissolves in the stomach, a kind of nanometer grade chitosan is provided, improve the activity of chitosan, solve problems such as absorption and dissolubility, simultaneously also its side effect be can reduce greatly, technical barrier and bottleneck that chitosan is applied to every field, especially medicine and the existence of functional food technical field at present fundamentally solved.
Another object of the present invention provides the preparation method of described nanoparticle.
A further object of the invention provides the application of described nanoparticle.
Purpose of the present invention is achieved by the following technical programs:
A kind of chitosan nano is provided, adopts the chitosan and sodium tripolyphosphate (TPP) the reaction spray drying after rotary evaporation concentrates that are dissolved in acetic acid solution to obtain.
Perhaps, adopt water-soluble chitosan and sodium tripolyphosphate (TPP) reaction back rotary evaporation concentrated spray drying to obtain.
The present invention provides the preparation method of described chitosan nano simultaneously, may further comprise the steps:
(1) chitosan is dissolved in acetic acid solution and gets chitosan solution, place standby;
(2) add in step (1) chitosan solution TPP react nanometer suspension liquid;
(3) the nanometer suspension liquid of step (2) gained concentrates through rotary evaporation;
(4) concentrated solution of step (3) gained carries out spray drying.
It is 0.5～2% acetic acid solution that step (1) can adopt volume by volume concentration; The acetic acid solution consumption is the chitosan of 100ml acetic acid solution dissolving 0.5～5g.
Preferably, can adopt volume by volume concentration is 0.5～1% acetic acid solution; The acetic acid solution consumption is the chitosan of 100ml acetic acid solution dissolving 1～3g.
The described TPP concentration of step (2) is 0.5～2mg/ml, preferred 0.75～1mg/ml; Consumption is to determine in 5: 1～10: 1 according to the mass ratio of chitosan and TPP.
The operation that adds TPP in step (2) chitosan solution is to carry out under the mechanical agitation condition of room temperature, 400～1000r/min, and the described response time is 30～60 minutes, and system has tangible blue opalescence to generate.
The described rotary evaporation of step (3) concentrates with reference to existing conventional technology, preferably carries out under 60～80 ℃ of temperature control conditions, nanometer suspension liquid is evaporated to volume is reduced to original half and gets final product.
The described spray drying condition of step (4) is: 140～190 ℃ of inlet temperatures, 80～110 ℃ of outlet temperatures, sample introduction flow velocity 500～1000ml/h, pressure 5～15atm, wind speed 0.65～0.95m
3/ min.
Preferred condition is 150～160 ℃ of inlet temperatures, 85～95 ℃ of outlet temperatures, sample introduction flow velocity 500～700ml/h, pressure 10～15atm, wind speed 0.65～0.8m
3/ min.
The present invention provides the preparation method of water-soluble chitosan nanoparticle simultaneously, specifically may further comprise the steps:
(1) with the water-soluble water-soluble chitosan solution that gets of water-soluble chitosan, place standby;
(2) add in step (1) the water-soluble chitosan solution sodium tripolyphosphate react nanometer suspension liquid;
(3) the nanometer suspension liquid rotary evaporation of step (2) gained concentrates;
(4) concentrated solution of step (3) gained carries out spray drying.
Preferably, the mass volume ratio of described water-soluble chitosan of step (1) and water is: 0.5～5g water-soluble chitosan: 100ml water.
The described tripolyphosphate na concn of step (2) is 0.5～2mg/ml, preferred 0.75～1mg/ml; Consumption is to determine in 5: 1～10: 1 according to the mass ratio of chitosan and sodium tripolyphosphate; The described response time is 30～60 minutes;
The described rotary evaporation of step (3) concentrates with reference to existing conventional technology, preferably carries out under 60～80 ℃ of temperature control conditions, nanometer suspension liquid is evaporated to volume is reduced to original half and gets final product.
The described spray drying condition of step (4) is: inlet temperature is 140～190 ℃, and outlet temperature is 80～110 ℃, and the sample introduction flow velocity is 500～1000ml/h, and pressure is 5～15atm, and wind speed is 0.65～0.95m
3/ min.
Preferred spray drying condition is: inlet temperature is 150～160 ℃, and outlet temperature is 85～95 ℃, and the sample introduction flow velocity is 500～700ml/h, and pressure is 10～15atm, and wind speed is 0.65～0.8m
3/ min.
The particle diameter that the present invention prepares chitosan nano and water-soluble chitosan nanoparticle usually＜1000nm, productive rate is 40～100%.
Chitosan nano that the present invention prepares or water-soluble chitosan nanoparticle can be applicable to preparation prevention and treatment hyperlipidemia or slimming health food or medicine aspect, have the advantage that dosage is little, useful effect is remarkable, toxic and side effects is little.
The invention has the beneficial effects as follows:
The present invention is directed to the dosage Da Yi that existing chitosan need use and cause untoward reaction; the defective of the overlong time that dissolves in the stomach; the utilization modern medicinal agents section of learning to do; adopt gentle the moon; sun polyelectrolyte charge interaction forms nano-complex; form the chitosan nano suspension technique effect of nanoparticle and deacetylation formation by research different anions polyelectrolyte; summary obtains technical solution of the present invention; the chitosan nano suspension is rotated evaporation and concentration; spray drying makes the chitosan nano with reducing weight and blood fat performance; significantly improve the activity of chitosan; solve problems such as its absorption and dissolubility difficulty; simultaneously also can reduce its side effect greatly, fundamentally solve chitosan and be applied to technical barrier and the technical bottleneck that the reducing weight and blood fat aspect exists at present.
The inventive method is simple, and cost is lower, is easy to promote.
Description of drawings
Fig. 1 chitosan nano particle size distribution figure
Fig. 2 chitosan nano transmission electron microscope picture
Fig. 3 water-soluble chitosan nanoparticle particle size distribution figure
Fig. 4 water-soluble chitosan nanoparticle transmission electron microscope picture
The specific embodiment
Further describe the present invention below in conjunction with specific embodiment, help those of ordinary skill in the art further to understand the present invention, but do not limit the present invention in any form.
The preparation of embodiment 1 chitosan nano
(1) the 25g chitosan (is made by oneself or commercial product according to existing method, the reference molecule amount is 30～500,000, but the realization of molecular weight and the technology of the present invention does not have direct relation, down together) be dissolved in the acetic acid solution of 1000ml0.5%, stirring is dissolved it fully, places standby;
(2) under the mechanical agitation condition of room temperature, 800r/min, in step (1) chitosan solution, add 0.75mg/ml TPP 100ml, reaction 45min, system has tangible blue opalescence to generate, and gets nanometer suspension liquid;
(3) the nanometer suspension liquid of step (2) gained is rotated evaporation and concentration under 70 ℃ of temperature control conditions, and volume is evaporated to long-pending half of nanometer suspension liquid and gets final product;
(4) concentrated solution of step (3) gained carries out spray drying, and it is 160 ℃ that inlet temperature is set, and outlet temperature is 85 ℃, and the sample introduction flow velocity is 600ml/h, and pressure is 13atm, and wind speed is 0.75m
3/ min.The spray drying gained is chitosan nano.
The preparation of embodiment 2 chitosan nanos
(1) the 25g chitosan being dissolved in the 1000ml volume by volume concentration is in 1% the acetic acid solution, stirs it is dissolved fully, places standby;
(2) under the mechanical agitation condition of room temperature, 600r/min, in above-mentioned chitosan solution, add 0.85mg/ml TPP 100ml, reaction 60min, system has tangible blue opalescence to generate, and gets nanometer suspension liquid;
(3) the nanometer suspension liquid of step (2) gained is rotated evaporation and concentration under 60 ℃ of temperature control conditions, and volume is evaporated to long-pending half of nanometer suspension liquid and gets final product;
(4) concentrated solution of step (3) gained carries out spray drying, and it is 160 ℃ that inlet temperature is set, and outlet temperature is 85 ℃, and the sample introduction flow velocity is 600ml/h, and pressure is 13atm, and wind speed is 0.75m
3/ min.The spray drying gained is chitosan nano.
The preparation of embodiment 3 chitosan nanos
(1) the 25g chitosan being dissolved in the 1000ml volume by volume concentration is in 2% the acetic acid solution, stirs it is dissolved fully, places standby;
(2) under the mechanical agitation condition of room temperature, 600r/min, in above-mentioned chitosan solution, add 2mg/ml TPP 120ml, reaction 60min, system has tangible blue opalescence to generate, and gets nanometer suspension liquid;
(3) the nanometer suspension liquid of step (2) gained is rotated evaporation and concentration under 75 ℃ of temperature control conditions, and volume is evaporated to long-pending half of nanometer suspension liquid and gets final product;
(4) concentrated solution of step (3) gained carries out spray drying, and it is 140 ℃ that inlet temperature is set, and outlet temperature is 110 ℃, and sample introduction flow velocity 1000ml/h, pressure are 5atm, and wind speed is 0.65m
3/ min.The spray drying gained is chitosan nano.
The preparation of embodiment 4 chitosan nanos
(1) the 50g chitosan being dissolved in the 1000ml volume by volume concentration is in 2% the acetic acid solution, stirs it is dissolved fully, places standby;
(2) under the mechanical agitation condition of room temperature, 600r/min, in above-mentioned chitosan solution, add 0.5mg/ml TPP 200ml, reaction 60min, system has tangible blue opalescence to generate, and gets nanometer suspension liquid;
(3) the nanometer suspension liquid of step (2) gained is rotated evaporation and concentration under 80 ℃ of temperature control conditions, and volume is evaporated to long-pending half of nanometer suspension liquid and gets final product;
(4) concentrated solution of step (3) gained carries out spray drying, and it is 190 ℃ that inlet temperature is set, and outlet temperature is 80 ℃, and sample introduction flow velocity 500ml/h, pressure are 15atm, and wind speed is 0.95m
3/ min.The spray drying gained is chitosan nano.
The preparation of embodiment 5 chitosan nanos
(1) the 25g chitosan is dissolved in the acetic acid solution of 1000ml 0.8%, stirring is dissolved it fully, places standby;
(2) under the mechanical agitation condition of room temperature, 700r/min, in above-mentioned chitosan solution, add 1mg/ml TPP 100ml, reaction 30min, system has tangible blue opalescence to generate, and gets nanometer suspension liquid;
(3) the nanometer suspension liquid of step (2) gained is rotated evaporation and concentration under 65 ℃ of temperature control conditions, and volume is evaporated to long-pending half of nanometer suspension liquid and gets final product;
(4) concentrated solution of step (3) gained carries out spray drying, and it is 160 ℃ that inlet temperature is set, and outlet temperature is 85 ℃, and the sample introduction flow velocity is 600ml/h, and pressure is 13atm, and wind speed is 0.75m
3/ min.The spray drying gained is chitosan nano.
Chitosan nano particle size distribution figure and chitosan nano transmission electron microscope picture are seen accompanying drawing 1 and accompanying drawing 2 respectively.By accompanying drawing 1 and accompanying drawing 2 as seen, chitosan nano is spherical, and smooth surface, centralized particle diameter.
The preparation of embodiment 6 water-soluble chitosan nanoparticles
(1) ((according to existing method self-control or commercial product, molecular weight is 5～100,000, but the realization of molecular weight and the technology of the present invention does not have direct relation) is dissolved in the 2500ml water, stirs it is dissolved fully, places standby with the 25g water-soluble chitosan;
(2) under the mechanical agitation condition of room temperature, 700r/min, in above-mentioned chitosan solution, add 1mg/ml TPP 100ml, reaction 30min, system has tangible blue opalescence to generate, and gets nanometer suspension liquid;
(3) the nanometer suspension liquid of step (2) gained is rotated evaporation and concentration under 65 ℃ of temperature control conditions, and volume is evaporated to long-pending half of nanometer suspension liquid and gets final product;
(4) concentrated solution of step (3) gained carries out spray drying, and it is 160 ℃ that inlet temperature is set, and outlet temperature is 85 ℃, and the sample introduction flow velocity is 600ml/h, and pressure is 13atm, and wind speed is 0.75m
3/ min.The spray drying gained is the water-soluble chitosan nanoparticle.
The preparation of embodiment 7 water-soluble chitosan nanoparticles
(1) the 25g water-soluble chitosan is dissolved in the 1250ml water, stirring is dissolved it fully, places standby;
(2) under the mechanical agitation condition of room temperature, 600r/min, in above-mentioned chitosan solution, add 0.85mg/ml TPP 100ml, reaction 60min, system has tangible blue opalescence to generate;
(3) the nanometer suspension liquid of step (2) gained is rotated evaporation and concentration under 60 ℃ of temperature control conditions, and volume is evaporated to long-pending half of nanometer suspension liquid and gets final product;
(4) concentrated solution of step (3) gained carries out spray drying, and it is 160 ℃ that inlet temperature is set, and outlet temperature is 85 ℃, and the sample introduction flow velocity is 600ml/h, and pressure is 13atm, and wind speed is 0.75m
3/ min.The spray drying gained is the water-soluble chitosan nanoparticle.
Water-soluble chitosan nanoparticle particle size distribution figure and water-soluble chitosan nanoparticle transmission electron microscope picture are seen accompanying drawing 3 and accompanying drawing 4 respectively.By accompanying drawing 3 and accompanying drawing 4 as seen, the water-soluble chitosan nanoparticle is for for spherical, centralized particle diameter.
Embodiment 8 chitosan nanos of the present invention and water-soluble chitosan nanoparticle antiobesity action and prevention and treatment hyperlipemia effect experiment
1. prevention effect on hyperlipemia experiment
(1) animal is selected and high fat prescription
Laboratory animal: 48 of the Kunming mouses (available from Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center) of choosing cleaning level, body weight is 30～50g, male and female half and half.
High lipoprotein emulsion prescription: get the 1g propylthiouracil and place the mortar porphyrize, device is deposited standby in addition.Get 20g Adeps Sus domestica, after heating (80 ℃) is melted, add the cholesterol of 10g porphyrize, the 1g propylthiouracil, fully stirring and dissolving adds the 5ml Tween 80 again, stirs, and adds 10% sodium deoxycholate liquid 20ml then, stirring and emulsifying, adding distil water promptly get high lipoprotein emulsion to 100ml.
(2) dosage grouping and administration time are determined
The Kunming mouse adaptability was fed 5 days, was divided into 8 groups at random, 6 every group.Chitosan nano of the present invention (CTS-NP) high and low dose group, water-soluble chitosan nanoparticle of the present invention (WSC-NP) high and low dose group are established in experiment.High dose group average appetite every day is decided to be 900mg/kg, and low dosage is 450mg/kg.
Other establishes normal control group, high fat matched group, chitosan (CTS) matched group and water-soluble chitosan (WSC) matched group, and wherein CTS matched group and WSC matched group dosage are with administration group high dose.Administration time is 15 days.The normal control group is irritated with normal saline, and high fat matched group is irritated with high lipoprotein emulsion, and the CTS matched group is irritated with common CTS liquid, and the WSC matched group is irritated with common WSC liquid.
(3) operating procedure
The blank group is fed with normal diet; Other group is irritated with high lipoprotein emulsion, gives given the test agent simultaneously, continuous 15 days; Experiment finishes posterior orbit and gets blood survey T-CHOL (TC), high density lipoprotein (HDL), low density lipoprotein, LDL (LDL) and triglyceride (TG), and the meansigma methods of the actual growth of each treated animal body weight is compared.
Above result's measurement data is handled through SPSS 16.0 statistical softwares, variance analysis, and the significance test of multisample mean comparison is checked with q.
(4) experimental result
A. the serum biochemistry experimental result is shown in Table 1.
As shown in Table 1, high fat matched group is compared with the normal control group, its TC, and TG, LDL all are higher than normal group, and difference has significance, the modeling success; CTS-NP and WSC-NP high and low dose group are compared the TG level and are all obviously reduced with the hyperlipidemia model group, and CTS-NP and WSC-NP low dose group and CTS matched group and WSC matched group do not have difference (P＞0.05).Illustrate and reduce CTS-NP and WSC-NP consumption, can reach the effect of prevention hyperlipemia equally.
Table 1CTS-NP and WSC-NP are to the influence of experimental mice serum biochemistry
B. body weight increment experimental result is shown in Table 2.
As shown in Table 2, CTS-NP compares the body weight increment with WSC-NP high and low dose group with the hyperlipidemia model group and all reduces, and the CTS-NP of low dose group and WSC-NP and CTS contrast and WSC contrast difference not obvious (P＞0.05).
Table 2CTS-NP and WSC-NP are to the influence of experimental mice body weight increment
2. treatment effect on hyperlipemia experiment
(1) animal is selected and high fat prescription
Laboratory animal is selected 64 of the SD rats (available from Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center) of SPF level, single sex (male), and body weight is between 180～220g.
High fat prescription is with 1..
(2) dosage grouping and administration time are determined
Adaptability was fed 5 days, was divided into 8 groups at random, 8 every group.
Experiment is established among the CTS-NP, low dose group, among the WSC-NP, small dose group.Dosage according to the common chitosan rat of bibliographical information is heavy for the 600mg/kg Mus, when preparing with this concentration, find that too thickness can't administration, being decreased to 150mg/kg Mus heavy prescription can, therefore selecting this concentration is middle dosage, and determines that low dose is heavy for the 75mg/kg Mus.
Other establishes normal control group, high fat matched group, CTS matched group and WSC matched group, and wherein CTS matched group and WSC matched group dosage are with dosage in the administration group.Administration time is 30 days.
(3) operating procedure
The blank group is given normal diet, and all the other each groups are all irritated with high lipoprotein emulsion, after 15 days, gets blood and surveys TC, TG, HDL, LDL, and whether observe model successful.Afterwards, high fat matched group continues to irritate with high lipoprotein emulsion, and all the other each groups are fed with normal diet respectively, and give given the test agent.Successive administration is 30 days then, and regularly weighs in.
Finish fasting in experiment and get blood after 16 hours, survey the variation of TC, TG, HDL, LDL.Measure plasma viscosity simultaneously.
Dissect corpse, take out liver, claim its weight in wet base, add the every 100mg liver weight in wet base of extracting solution (1: 1 volume ratio of chloroform and methanol) and add 2ml, be ground into homogenate, add extracting solution again, mixing, centrifugal, survey its TC, TG.Get kidney week, all fat of testis simultaneously, weigh.
Above result's measurement data is handled through the SPSS16.0 statistical software, variance analysis, and the significance test of multisample mean comparison is checked with q
(4) experimental result
A. the serum biochemistry experimental result is shown in Table 3.
As shown in Table 3, hyperlipidemia model group and normal control group compare, and TC, LDL-C all obviously raise, and prove that the animal modeling is successful on the whole; Compare with model group, among CTS-NP and the WSC-NP, small dose group all makes TC and LDL obviously descend, and with the CTS matched group, the WSC matched group is compared, difference has significance (P＜0.05).
Table 3CTS-NP and WSC-NP are to the influence of experimental rat serum biochemistry
B. the plasma viscosity experimental result is shown in Table 4.
As shown in Table 4, among the CTS-NP, among small dose group and the WSC-NP, small dose group compares with the hyperlipidemia model group, its plasma viscosity all significantly descends.And with the CTS matched group, the WSC matched group is compared better effects if (P＜0.05).
Table 4CTS-NP and WSC-NP are to the influence of experimental rat plasma viscosity
C. body weight increment experimental result is shown in Table 5.
As shown in Table 5, among CTS-NP and the WSC-NP, small dose group compares with the normal control group, and the body weight increment is reduced.The CTS-NP of small dose group and WSC-NP and CTS matched group, the WSC matched group is compared, and difference does not have significance (P＞0.05).Show that CTS-NP compares with CTS, WSC matched group with WSC-NP, using dosage reduces half, can reach fat-reducing effect.
Table 5CTS-NP and WSC-NP are to the influence of experimental rat body weight
D. liver weight, liver index, body fat experimental result are shown in Table 6.
As shown in Table 6, CTS-NP compares with the normal control group with high fat matched group with the big low dose of WSC-NP, all no difference of science of statistics.
Table 6CTS-NP and WSC-NP are to the influence of experimental rat liver weight and body fat
E. the liver biochemistry experimental result is shown in Table 7.
As shown in Table 7, the TC of hyperlipidemia model group and LDL all are higher than normal group; Among CTS-NP and the WSC-NP, low dose all makes TC reduce, HDL raises.Illustrate that CTS-NP and WSC-NP all can reduce the deposition of TC in the liver.
Table 7CTS-NP and WSC-NP are to the influence of experimental rat liver biochemistry
F. serum and liver SOD experimental result are shown in Table 8.
As shown in Table 8, the dosage group is compared with model control group among the CTS-NP, and activity of SOD in serum obviously strengthens, and all the other each groups do not have significant difference.Liver SOD numerical value is less, does not have statistical significance.
Table 8CTS-NP and WSC-NP are to the influence of experimental rat serum regulating liver-QI SOD
Chitosan nano of the present invention (CTS-NP) and water-soluble chitosan nanoparticle (WSC-NP) can significantly reduce plasma viscosity, simultaneously TC, LDL are also had good reduction effect, illustrate that its lipid-lowering effect is better.CTS-NP and WSC-NP also alleviate to some extent to body weight, have solved the big problem of present chitosan taking dose.And the activity of SOD in serum value strengthens greatly, illustrates that CTS-NP and WSC-NP have very strong antioxidant activity.
Claims (6)
1. a chitosan nano is characterized in that adopting the chitosan and the sodium tripolyphosphate reaction back rotary evaporation concentrated spray drying that are dissolved in acetic acid solution to obtain; Its preparation method may further comprise the steps:
(1) chitosan being dissolved in acetic acid solution, to get chitosan solution standby;
(2) add in step (1) chitosan solution sodium tripolyphosphate react nanometer suspension liquid; The operation that adds sodium tripolyphosphate in the chitosan solution is to carry out under the mechanical agitation condition of room temperature, 400～1000r/min;
(3) the nanometer suspension liquid rotary evaporation of step (2) gained concentrates;
(4) concentrated solution of step (3) gained carries out spray drying promptly;
Wherein, the described acetic acid solution volume by volume concentration of step (1) is 0.5～2%; The acetic acid solution consumption is every 100ml acetic acid solution dissolving 0.5～5g chitosan; The described tripolyphosphate na concn of step (2) is 0.5～2mg/ml, and consumption is to determine in 5: 1～10: 1 according to the volume ratio of chitosan solution and sodium tripolyphosphate; The described response time is 30～60 minutes; The described rotary evaporation thickening temperature of step (3) is 60～80 ℃; The described spray drying condition of step (4) is 140～190 ℃ of inlet temperatures, 80～110 ℃ of outlet temperatures, sample introduction flow velocity 500～1000ml/h, pressure 5～15atm, wind speed 0.65～0.95m
3/ min.
2. according to the preparation method of the described chitosan nano of claim 1, it is characterized in that step (1) acetic acid solution volume by volume concentration is 0.5～1%; The acetic acid solution consumption is every 100ml acetic acid solution dissolving 1～3g chitosan; The described tripolyphosphate na concn of step (2) is 0.5～1mg/ml; The described spray drying condition of step (4) is 150～160 ℃ of inlet temperatures, 85～95 ℃ of outlet temperatures, sample introduction flow velocity 500～700ml/h, pressure 10～15atm, wind speed 0.65～0.8m
3/ min.
3. the application of the described chitosan nano of claim 1 is characterized in that the health food or the medicine aspect that are applied to prepare prevention and treat hyperlipidemia or controlling body weight.
4. a water-soluble chitosan nanoparticle is characterized in that water-soluble chitosan dissolving back and sodium tripolyphosphate reaction rotary evaporation concentrated spray drying are obtained; Its preparation method may further comprise the steps:
(1) with the water-soluble water-soluble chitosan solution that gets of water-soluble chitosan;
(2) add in step (1) the water-soluble chitosan solution sodium tripolyphosphate react nanometer suspension liquid;
(3) the nanometer suspension liquid rotary evaporation of step (2) gained concentrates;
(4) concentrated solution of step (3) gained carries out spray drying promptly;
Wherein, the mass volume ratio of described water-soluble chitosan of step (1) and water is 0.5～5g/100ml; The described tripolyphosphate na concn of step (2) is 0.5～2mg/ml, and consumption is to determine in 5: 1～10: 1 according to the mass ratio of chitosan and sodium tripolyphosphate; The described response time is 30～60 minutes; The described rotary evaporation thickening temperature of step (3) is 60～80 ℃; The described spray drying condition of step (4) is 140～190 ℃ of inlet temperatures, 80～110 ℃ of outlet temperatures, sample introduction flow velocity 500～1000ml/h, pressure 5～15atm, wind speed 0.65～0.95m
3/ min.
5. according to the preparation method of the described chitosan nano of claim 4, the mass volume ratio that it is characterized in that described water-soluble chitosan of step (1) and water is 1～3g: 100ml; The described tripolyphosphate na concn of step (2) is 0.75～1mg/ml; The described spray drying condition of step (4) is 150～160 ℃ of inlet temperatures, 85～95 ℃ of outlet temperatures, sample introduction flow velocity 500～700ml/h, pressure 10～15atm, wind speed 0.65～0.8m
3/ min.
6. the application of the described water-soluble chitosan nanoparticle of claim 4 is characterized in that the health food or the medicine aspect that are applied to prepare prevention and treat hyperlipidemia or controlling body weight.
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| CN102010512B (en) * | 2010-10-12 | 2012-12-12 | 广东药学院 | Preparation method and application of water-soluble chitosan micro-particles |
| CN101978954B (en) * | 2010-10-12 | 2012-02-15 | 广东药学院 | Preparation and application of chitosan particles |
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| CN102389398A (en) * | 2011-08-26 | 2012-03-28 | 广东药学院 | Chitosan-loaded capsaicin microspheres, preparation method thereof, and application thereof |
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2009
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