CN101708198B - Aruncus sylvester Kostel. ex Maxim. root extract with antioxidant activity - Google Patents
Aruncus sylvester Kostel. ex Maxim. root extract with antioxidant activity Download PDFInfo
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- CN101708198B CN101708198B CN2009102180027A CN200910218002A CN101708198B CN 101708198 B CN101708198 B CN 101708198B CN 2009102180027 A CN2009102180027 A CN 2009102180027A CN 200910218002 A CN200910218002 A CN 200910218002A CN 101708198 B CN101708198 B CN 101708198B
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Abstract
The invention belongs to the technical field of research and development of medical products. The invention relates to an Aruncus sylvester Kostel. ex Maxim. root extract with antioxidant activity, which is prepared by using the Aruncus sylvester Kostel. ex Maxim. root as a raw material. The Aruncus sylvester Kostel. ex Maxim. root is picked up, leached and extracted in ethanol at the room temperature and undergoes ultrasonic extraction to give the Aruncus sylvester Kostel. ex Maxim. extract. The Aruncus sylvester Kostel. ex Maxim. root extract has a strong effect of removing DPPH, can be used as a complementary antioxidant material and helps the moving organisms to reduce the generation of free radicals or speed up the removal of the free radicals so as to resist the side effect of the free radicals, so that the Aruncus sylvester Kostel. ex Maxim. root extract is good for the health of ordinary people and athletes and can delay the happening of exercise-induced fatigue and quicken the recovery of physical fitness.
Description
Technical field
Medical product research and development technical field under the present invention.The present invention relates to Aruncus dioicus (Aruncus sylvesterKostel.ex Maxim.) root is a kind of aruncus dioicus extract with antioxidant activity of feedstock production.
Background technology
The present invention relates to Aruncus dioicus (Aruncus sylvester Kostel.ex Maxim.) root is a kind of aruncus dioicus extract with antioxidant activity of feedstock production.Get Radix seu Herba Astilbes rivularis, with after the lixiviate under the ethanol room temperature again supersound extraction get aruncus dioicus extract, this extract has very strong scavenging action to DPPH.Can be used for removing the various free radicals that human body constantly produces in the oxidative metabolism process of vital movement, protection human body cell, protection immune system are to avoid the generation of disease.Additional antioxidant helps the motion body to be reduced the generation of free radical or quickens its removing, with side effect to free radical resisting, thereby all useful to common people and athletic health, but the delayed motion fatigue takes place and the quickening physical efficiency recovery.The present invention is beneficial to suitability for industrialized production.
Summary of the invention
1. aruncus dioicus extract with antioxidant activity is characterized in that preparing this extract and comprises following process:
(1) get Radix seu Herba Astilbes rivularis, choose the roguing thing, washing is dried or is dried, and pulverizes, and doubly measures under the ethanol room temperature of 60%-70% ultrasonic 1-1.5h behind the lixiviate 4-6h with 8-10, and temperature is that 30 ℃ of power are 80W, filters respectively once with silk and filter paper then, obtains filtrate;
(2) filtering residue of Radix seu Herba Astilbes rivularis after handle (1) repeats (1) step respectively once with the ethanol of 6 times of amounts and 4 times of amount 60%-70% respectively again, obtains filtrate respectively, merges three times filtrate;
(3), get aruncus dioicus extract with above-mentioned (2) filtrate recycling ethanol.
2, the purposes of the described aruncus dioicus extract of claim 1 is characterized in that this extract is used to prepare the preparation that DPPH is had scavenging action.
According to the present invention, " % " among the present invention is percentage by weight.
The specific embodiment
The following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
(1) get Radix seu Herba Astilbes rivularis, choose the roguing thing, washing is dried or is dried, and pulverizes, and measures under 70% the ethanol room temperature ultrasonic 1.5h behind the lixiviate 6h with 10 times, and temperature is that 30 ℃ of power are 80W, filters respectively once with silk and filter paper then, obtains filtrate;
(2) filtering residue of Radix seu Herba Astilbes rivularis after handle (1) repeats (1) step respectively once with the ethanol of 6 times of amounts and 4 times of amounts 70% respectively again, obtains filtrate respectively, merges three times filtrate;
(3), get aruncus dioicus extract with above-mentioned (2) filtrate recycling ethanol.
Embodiment 2:
(1) get Radix seu Herba Astilbes rivularis, choose the roguing thing, washing is dried or is dried, and pulverizes, and measures under 60% the ethanol room temperature ultrasonic 1h behind the lixiviate 4h with 8 times, and temperature is that 30 ℃ of power are 80W, filters respectively once with silk and filter paper then, obtains filtrate;
(2) filtering residue of Radix seu Herba Astilbes rivularis after handle (1) repeats (1) step respectively once with the ethanol of 6 times of amounts and 4 times of amounts 60% respectively again, obtains filtrate respectively, merges three times filtrate;
(3), get aruncus dioicus extract with above-mentioned (2) filtrate recycling ethanol.
The test of pharmacological testing Radix seu Herba Astilbes rivularis antioxidation
1 material, reagent
1.1 material
Aruncus dioicus extract
1.2 reagent
DPPH (Sigma company), analysis water are ultra-pure water.
2 experimental techniques
2.1 test specimen: aruncus dioicus extract.
2.2 the preparation of sample solution: precision takes by weighing that aruncus dioicus extract 0.4g places 50ml tool plug volumetric flask in 2.1, adds an amount of distilled water, seals ultrasonic 30min, after treating its dissolving fully, add distilled water again and be settled to 50ml, obtain the sample mother solution, concentration is 8mg/ml, and is standby.In the sample mother solution, pipetting volume with the liquid-transfering gun precision is 0.0625,0.125,0.25,0.5,1,2,4, the sample mother solution of 8ml, obtaining concentration with the distilled water standardize solution in the 10ml volumetric flask is 0.05,0.1,0.2,0.4,0.8,1.6,3.2, the sample solution of 6.4mg/ml.
2.3DPPH the preparation of solution: DPPH sample 0.01g decided in accurate title, adds dehydrated alcohol and be settled in the 250ml volumetric flask, obtains DPPH solution, and concentration is 0.04mg/ml.
2.4 Radix seu Herba Astilbes rivularis solution is to the scavenging action of DPPH: the Radix seu Herba Astilbes rivularis solution that accurately pipettes variable concentrations is mixed in the different test tubes with DPPH solution and ethanol solution equal-volume respectively, shake up, place 30min in the dark, under 515nm, measure its absorbance and be respectively A
iAnd A
jOther gets distilled water and DPPH solution equal-volume is mixed in the test tube, and recording absorbance is A.Wherein be mixed into the blank test with distilled water and dehydrated alcohol.And by following formula calculating clearance rate.
Clearance rate=(A-A
i+ A
j)/A * 100%
2.5 Radix seu Herba Astilbes rivularis 50% (IC
50) calculating of inhibition concentration: utilize the SPSS16.0 software analysis, draw IC
50Value.
3 results
3.1 the Radix seu Herba Astilbes rivularis solution of variable concentrations is to the elimination factor of DPPH
Table 1 variable concentrations Radix seu Herba Astilbes rivularis is removed DPPH result
Table1?Syringa?reticulata?different?concentrations?of?DPPH?to?remove?the?results?of?Shannon
3.2 utilize the data in the SPSS16.0 statistical analysis 3.1
Table 2 aruncus dioicus extract 50% (IC
50) inhibition concentration
Table2?Syringa?reticulata?extract?Shannon?50％(IC50)inhibitory?concentration
a.A?heterogeneity?factor?is?used
Draw aruncus dioicus extract 50% (IC from table 2
50) inhibition concentration is 0.009mg/ml.
Claims (2)
1. aruncus dioicus extract with antioxidant activity is characterized in that preparing this extract and comprises following process:
(1) get Radix seu Herba Astilbes rivularis, choose the roguing thing, washing is dried or is dried, and pulverizes, and doubly measures under the ethanol room temperature of 60%-70% ultrasonic 1-1.5h behind the lixiviate 4-6h with 8-10, and temperature is that 30 ℃ of power are 80W, filters respectively once with silk and filter paper then, obtains filtrate;
(2) filtering residue of Radix seu Herba Astilbes rivularis after handle (1) repeats (1) step respectively once with the ethanol of 6 times of amounts and 4 times of amount 60%-70% respectively again, obtains filtrate respectively, merges three times filtrate;
(3), get aruncus dioicus extract with above-mentioned (2) filtrate recycling ethanol.
2. the purposes of the described aruncus dioicus extract of claim 1 is characterized in that this extract is used to prepare the preparation that DPPH is had scavenging action.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102180027A CN101708198B (en) | 2009-12-15 | 2009-12-15 | Aruncus sylvester Kostel. ex Maxim. root extract with antioxidant activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102180027A CN101708198B (en) | 2009-12-15 | 2009-12-15 | Aruncus sylvester Kostel. ex Maxim. root extract with antioxidant activity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101708198A CN101708198A (en) | 2010-05-19 |
| CN101708198B true CN101708198B (en) | 2011-12-14 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817828B (en) * | 2010-03-01 | 2011-11-30 | 山东大学威海分校 | Preparation of bergenin in aruneus dioicus |
| CN107602633B (en) * | 2017-09-11 | 2020-11-27 | 天津大学 | 1, 3, 6-tri-O- (E) -caffeoyl-beta-D-glucopyranose and extraction and separation method |
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