CN101703562A - Eucommia bark ultra-micro powder with activity of resisting osteoporosis and preparation method thereof - Google Patents
Eucommia bark ultra-micro powder with activity of resisting osteoporosis and preparation method thereof Download PDFInfo
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- CN101703562A CN101703562A CN200910186209A CN200910186209A CN101703562A CN 101703562 A CN101703562 A CN 101703562A CN 200910186209 A CN200910186209 A CN 200910186209A CN 200910186209 A CN200910186209 A CN 200910186209A CN 101703562 A CN101703562 A CN 101703562A
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Abstract
The invention relates to eucommia bark ultra-micro powder with activity of resisting osteoporosis and a preparation method thereof. The preparation method is characterized by comprising the following steps of: evenly stirring an eucommia bark thread crude product with brine; soaking the mixture for 24 hours; drying the mixture in a drying oven at the temperature of 60 DEG C; baking the mixture continuously for 30 minutes at the temperature of 180 DEG C; and cooling the baked mixture, and grinding the mixture into powder with the particle size of 10 to 100 mu m by an ultra-micro grinder. The technical effect of the eucommia bark ultra-micro powder is that the obtained eucommia bark ultra-micro powder has a grey brown color and good dispersity, and has good curative effect, quick response and high medicament utilization ratio compared with the conventional powder.
Description
Technical field
The present invention relates to a kind of medicine for the treatment of osteoporosis, is the medicine of feedstock production with the Cortex Eucommiae specifically, the invention still further relates to the preparation method of this medicine, belongs to the field of Chinese medicines.
Background technology
Osteoporosis is impaired with the osseous tissue microstructure, bone ore deposit composition and bone matrix equal proportion ground constantly reduce, the sclerotin attenuation, bone trabecula quantity reduces, the disease of a kind of general metabolism obstacle that increase of bone fragility and risk of fractures degree raise, be a kind of commonly encountered diseases of postmenopausal women and old people, become one of human modal disease.China's traditional Chinese medical science thinks that osteoporotic basic reason is " suffering from a deficiency of the kidney ".The Cortex Eucommiae is the bark of Eucommiaceae plant Cortex Eucommiae Eucommiae ulmoides Oliv., it is traditional rare Chinese medicine of China, give birth to and use or saline stir-fry usefulness, its property is sweet, and temperature is returned liver, kidney channel, has invigorating the liver and kidney, bone and muscle strengthening, antiabortive effect clinically is used for diseases such as hypertension, lumbago due to renal deficiency, weakness of the waist and knees, osteoporosis.
Traditional Cortex Eucommiae application method is mainly decoction, and the Cortex Eucommiae single medicinal material preparation of selling in the market " full eucommia bark capsules " is to utilize 90% medicinal material extract extractum in addition, and 10% breaks into fine powder, and the two mixed pelletization incapsulates and makes.Above-mentioned using method is not owing to cause the plant cell wall breaking of the Cortex Eucommiae, and therefore, intracellular active chemical is difficult for stripping, and it is slow to take effect during treatment, and utilization ratio of drug is low.
Summary of the invention
The objective of the invention is to provides a kind of eucommia bark ultra-micro powder with activity of resisting osteoporosis at top described deficiency, good effect, instant effect when making it to the treatment osteoporosis.
Another object of the present invention is to provide a kind of preparation method with eucommia bark ultra-micro powder of activity of resisting osteoporosis.
The technical solution adopted in the present invention is:
A kind of eucommia bark ultra-micro powder with activity of resisting osteoporosis is characterized in that: diameter of particle is 10-100 μ m, and the order number is the 150-1500 order, and it is to be made by following steps,
(1) concocts: get Cortex Eucommiae silk and give birth to product, get the salt of 2% medical material weight again, use water dissolution, the weight ratio of salt and water is 1: 10, saline and Cortex Eucommiae silk stir evenly and soaked into 20-30 hour, put 50-70 ℃ of oven dry in the baking oven, under 160-200 ℃ of temperature, continue baking 25-35 minute again, with burnt brown or charred ashes color, fracture of wire degree of being.
(2) pulverize: step (1) gained step is broken into the powder body that particle diameter is 10-100 μ m through super micron mill.
In the described step (1), the diameter of Cortex Eucommiae silk is 2～3mm.
The breaking method of described step (2) can be that cut is pulverized, and the formula of hitting is pulverized, and the mill formula is pulverized (comprising ball milling, vibromill, stirring mill), comminution by gas stream etc.
A kind of preparation method with eucommia bark ultra-micro powder of activity of resisting osteoporosis is characterized in that: it is to be made by following steps,
(1) concocts: get Cortex Eucommiae silk and give birth to product, get the salt of 2% medical material weight again, use water dissolution, the weight ratio of salt and water is 1: 10, saline and Cortex Eucommiae silk stir evenly and soaked into 20-30 hour, put 50-70 ℃ of oven dry in the baking oven, under 160-200 ℃ of temperature, continue baking 25-35 minute again, with burnt brown or charred ashes color, fracture of wire degree of being.
(2) pulverize: step (1) gained step is broken into the powder body that particle diameter is 10-100 μ m through super micron mill.
In the described step (1), the diameter of Cortex Eucommiae silk is 2～3mm.
The breaking method of described step (2) comprises the cut pulverizing, and the formula of hitting is pulverized, and the mill formula is pulverized (comprising ball milling, vibromill, stirring mill), comminution by gas stream etc.; Pulverize environment and comprise pulverize at low temperature and room temperature pulverizing.
Beneficial effect of the present invention: the resulting eucommia bark ultra-micro powder of the present invention is uniform taupe, good dispersion, can be directly as powder or incapsulate and take, because Cortex Eucommiae cell wall breaking rate after micronizing reaches 90%, help the stripping of validation effect composition in the cell, the more traditional medicated powder of its drug effect is remarkable, waiting dosage under the medicated strip spare only for 50%～60% of traditional medicated powder, can save medical material greatly.
The specific embodiment
Embodiment one:
Cut-off directly is that the Cortex Eucommiae silk of 2mm is given birth to product 1kg, and other gets 20g Sal and is dissolved in the 200mL water, slowly adds in the medical material saline and continuous stirring and evenly mixing, airtight infiltration 24 hours.Place 60 ℃ of oven dry of baking oven, continue baking 30 minutes again under 180 ℃ of temperature, put cold back and be crushed to the ultra-micro powder that particle diameter is 10-100 μ m with the micronizing instrument, grinding mode is that cut is pulverized, and pulverizing environment is that room temperature is pulverized.
Embodiment two:
Cut-off directly is that the Cortex Eucommiae silk of 3mm is given birth to product 1kg, and other gets 20g Sal and is dissolved in the 200mL water, slowly adds in the medical material saline and continuous stirring and evenly mixing, airtight infiltration 30 hours.Place 50 ℃ of oven dry of baking oven, continue baking 25 minutes again under 200 ℃ of temperature, put cold back and be crushed to the ultra-micro powder that particle diameter is 10-100 μ m with the micronizing instrument, grinding mode is pulverized for the mill formula, and the pulverizing environment is a pulverize at low temperature.
Embodiment three:
Cut-off directly is that the Cortex Eucommiae silk of 2.5mm is given birth to product 1kg, and other gets 20g Sal and is dissolved in the 200mL water, slowly adds in the medical material saline and continuous stirring and evenly mixing, airtight infiltration 20 hours.Place 70 ℃ of oven dry of baking oven, continue baking 35 minutes again under 160 ℃ of temperature, put cold back and be crushed to the ultra-micro powder that particle diameter is 10-100 μ m with the micronizing instrument, grinding mode is pulverized for hitting formula, and pulverizing environment is that room temperature is pulverized.
Embodiment four:
Cut-off directly is that the Cortex Eucommiae silk of 2mm is given birth to product 1kg, and other gets 20g Sal and is dissolved in the 200mL water, slowly adds in the medical material saline and continuous stirring and evenly mixing, airtight infiltration 25 hours.Place 66 ℃ of oven dry of baking oven, continue baking 25 minutes again under 190 ℃ of temperature, put cold back and be crushed to the ultra-micro powder that particle diameter is 10-100 μ m with the micronizing instrument, grinding mode is a comminution by gas stream, and the pulverizing environment is a pulverize at low temperature.
The present invention has carried out drug effect relatively with powder and following two reference examples of embodiment one gained, adopts old rats pastille serum to cultivate rabbit osteoblast method, and relatively it is to the osteoblastic proliferation function of rabbit by mtt assay, and method is as follows:
Reference examples one:
Cut-off directly is that the Cortex Eucommiae silk of 2mm is given birth to product 1kg, and other gets 20g Sal and is dissolved in the 200mL water, slowly adds in the medical material saline and continuous stirring and evenly mixing, airtight infiltration 24 hours.Place 60 ℃ of oven dry of baking oven, under 180 ℃ of temperature, continue baking 30 minutes again, put cold back and be ground into fine powder (particle diameter is 125-180 μ m, and the order number is the 80-120 order) with traditional grinding mode.
Reference examples two:
Cut-off directly is that the Cortex Eucommiae silk of 3mm is given birth to product 1kg, other gets 20g Sal and is dissolved in the 200mL water, slowly add in the medical material saline and continuous stirring and evenly mixing, airtight infiltration 24 hours. place 60 ℃ of oven dry of baking oven (about 8 hours), under 180 ℃ of temperature, continue baking 30 minutes again, put cold back and be ground into middle powder (particle diameter is 180-250 μ m, and the order number is the 65-80 order) with traditional grinding mode.
1. rat pastille serum preparation: above three kinds of different-grain diameter Eucommia Bark water furnishing pasty states, every day early respectively, evening, each once pressed the rat that dry powder dosage [3.0g/ (kgd)] amount filling stomach is given 18 monthly ages, continuous irrigation stomach 5 times, last filling stomach is anaesthetized after half an hour and is got blood, separation of serum, adopt the D8900 serum-free medium to add not commensurability administration rat blood serum and be made into the culture medium that contains different pastille serum respectively, by make the not D8900 culture medium of pastille serum of variable concentrations with quadrat method, above culture medium-40 ℃ preservation is standby with physiologic saline for substitute Cortex Eucommiae medicated powder for matched group.
2. the rabbit osteoblast is cultivated: press literature method and the above variable concentrations pastille in adherent back is cultivated in rabbit the 2nd generation osteoblast digestion dyeing reached the not D8900 culture medium of pastille serum, other establishes serum-free D8900 culture medium and sodium fluoride D8900 serum-free medium respectively as normal group and positive drug group.Cultivate by literature method.Cultivate the end back and measure each hole absorbance with microplate reader (490nm).The results are shown in following table.
The result shows: powder is suitable to the rabbit activity of osteoblast proliferation with fine powder group Mus pastille serum in the Cortex Eucommiae, omits from the data fine powder, but does not have statistical significance.Yet superfine powder group powder group P＜0.01 during activity obviously is better than under identical serum-concentration condition.And analyze from culture medium pastille serum-concentration, " D8900 adds 4% pastille Mus serum (superfine powder group) " activity is equivalent to " D8900 adds 8% pastille Mus serum (middle powder group) ", and " D8900 adds 8% pastille Mus serum (superfine powder group) " activity is equivalent to " D8900 adds 16% pastille Mus serum (middle powder group) ".This shows that under the Isodose medical material situation, effect is better than middle powder and fine powder group far away to superfine powder group rat pastille serum to the rabbit osteoblastic proliferation.Under the equal drug effect condition, superfine powder group rat pastille serum consumption only is 50%～60% of a middle powder group, saves medical material and can reach 40%～50%.
Cortex Eucommiae different-grain diameter powder to rabbit activity of osteoblast proliferation influence (min ± s, n=10)
Compare with the D8900 group: * P＜0.05, * * P＜0.01
Add the NaF group relatively with D8900: △ P＜0.05, △ △ P＜0.01
Add not pastille Mus serum group comparison of same concentrations with D8900: ▲ P＜0.05, ▲ ▲ P＜0.01
Add same concentrations pastille Mus serum (middle powder group) relatively with D8900: zero P＜0.05, zero zero P＜0.01.
Claims (4)
1. eucommia bark ultra-micro powder with activity of resisting osteoporosis, it is characterized in that: diameter of particle is 10-100 μ m, and the order number is the 150-1500 order, and it is to be made by following steps,
(1) concocts: get Cortex Eucommiae silk and give birth to product, get the salt of 2% medical material weight again, use water dissolution, the weight ratio of salt and water is 1: 10, saline and Cortex Eucommiae silk stir evenly and soaked into 20-30 hour, put 50-70 ℃ of oven dry in the baking oven, under 160-200 ℃ of temperature, continue baking 25-35 minute again, with burnt brown or charred ashes color, fracture of wire degree of being;
(2) pulverize: step (1) gained step is broken into the powder body that particle diameter is 10-100 μ m through super micron mill.
2. a kind of eucommia bark ultra-micro powder according to claim 1 with activity of resisting osteoporosis, it is characterized in that: the breaking method of described step (2) can be that cut is pulverized, the formula of hitting is pulverized, and the mill formula is pulverized (comprising ball milling, vibromill, stirring mill), comminution by gas stream etc.
3. preparation method with eucommia bark ultra-micro powder of activity of resisting osteoporosis, it is characterized in that: it is to be made by following steps,
(1) concocts: get Cortex Eucommiae silk and give birth to product, get the salt of 2% medical material weight again, use water dissolution, the weight ratio of salt and water is 1: 10, saline and Cortex Eucommiae silk stir evenly and soaked into 20-30 hour, put 50-70 ℃ of oven dry in the baking oven, under 160-200 ℃ of temperature, continue baking 25-35 minute again, with burnt brown or charred ashes color, fracture of wire degree of being;
(2) pulverize: step (1) gained step is broken into the powder body that particle diameter is 10-100 μ m through super micron mill.
4. a kind of preparation method according to claim 3 with eucommia bark ultra-micro powder of activity of resisting osteoporosis, it is characterized in that: the breaking method of described step (2) comprises the cut pulverizing, the formula of hitting is pulverized, and the mill formula is pulverized (comprising ball milling, vibromill, stirring mill), comminution by gas stream etc.; Pulverize environment and comprise pulverize at low temperature and room temperature pulverizing.
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| CN200910186209A CN101703562A (en) | 2009-10-13 | 2009-10-13 | Eucommia bark ultra-micro powder with activity of resisting osteoporosis and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104490984A (en) * | 2014-12-25 | 2015-04-08 | 井冈山大学 | Preparation method of eucommia bark superfine powder |
| CN107752017A (en) * | 2017-11-09 | 2018-03-06 | 王慧 | A kind of preparation method of ginseng submicron powder soft capsule |
-
2009
- 2009-10-13 CN CN200910186209A patent/CN101703562A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104490984A (en) * | 2014-12-25 | 2015-04-08 | 井冈山大学 | Preparation method of eucommia bark superfine powder |
| CN107752017A (en) * | 2017-11-09 | 2018-03-06 | 王慧 | A kind of preparation method of ginseng submicron powder soft capsule |
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Open date: 20100512 |