CN101701186A - Automatic fecal cast-off cell extracting device - Google Patents
Automatic fecal cast-off cell extracting device Download PDFInfo
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- CN101701186A CN101701186A CN 200910225360 CN200910225360A CN101701186A CN 101701186 A CN101701186 A CN 101701186A CN 200910225360 CN200910225360 CN 200910225360 CN 200910225360 A CN200910225360 A CN 200910225360A CN 101701186 A CN101701186 A CN 101701186A
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Abstract
The invention discloses an automatic fecal cast-off cell extracting device, and is characterized in that the device comprises a sample collector, a sample processing device, a cell enriching device and an auxiliary automated mechanical device thereof; the sample collector comprises a sample collecting tube sealing cover and a sample collecting tube; the inside wall of the sample collecting tube sealing cover is provided with a sampling spoon; the sampling collecting tube sealing cover is installed at the upper end of the sample collecting tube; the lower end of the sample collecting tube is connected with the upper end of the sample processing device; the lower end pipe orifice of the sample processing device is connected into the cell enriching device through pipelines; the auxiliary automated mechanical device is divided into an inversion and reset power system of the sample processing device, an up-and-down vibration power system of the sample processing device, a fluid flow power system and an auxiliary system of the cell enriching device. The device improves the tolerance of inspectors for fecal samples, has the characteristics of automated completion by instruments in the whole processing process, no pollution, high accuracy, good specificity, and the like, and has wide clinical application range.
Description
Present patent application is applied for invention and utility model patent simultaneously.
Technical field
The present invention relates to biological technical field, particularly relate to a kind of antigen antibody reaction and self-reacting device thereof.
Background technology
Large bowel cancer is meant the cancer at positions such as rectum, sigmoid colon, caecum, colon, wherein much more to see with the rectum cancer and colorectal carcinoma, and be one of modal malignant tumor of digestive tract, its incidence and mortality is ascendant trend year by year.Large bowel cancer occupies second at American-European developed country sickness rate, and lethality rate is higher by 10% than other cancers, is the highest malignant tumour of sickness rate in the non-smoking crowd.In recent years, along with the raising of Chinese economic development and living standards of the people, diet formula is towards the diet composition transition of, high protein higher fatty acid with meat, egg, milk etc. and low VITAMIN, and the sickness rate of large bowel cancer has the trend that increases year by year.
Large bowel cancer has the grade malignancy height, shifts characteristics such as morning, so the key of large bowel cancer treatment is early diagnosis.Since oneth century, rectum cancer pathological staging method is numerous, but basic according to mainly being made of three big key elements: promptly the primary tumor scope, the regional lymph node metastasis and the distant metastasis situation of denying arranged.There is data to show that large bowel cancer postoperative 5 annual survival rates are respectively Duke ' the s A phase: 81%～85%; Duke ' the sB phase: 64%～78%; Duke ' the s C phase: 27%～33%; Duke ' the s D phase: 5%～14%, illustrate after making a definite diagnosis large bowel cancer in early days helps performing the operation and cure.
Table 1 large bowel cancer Duke ' s is system by stages
Because the morbidity organ of large bowel cancer is easy to detect, its generation process and happening part are regular governed, should set up effective preventative intervention mechanism at the large bowel cancer generating process.Being applied to clinical large bowel cancer detection technique at present mainly contains: (1) ight soil occult blood detects; (2) splanchnoscopy.
The existing clinical detection technique relative merits of table 2 relatively
Compare with other detection methods, utilizing ight soil to carry out the large bowel cancer detection has many important advantages.Ight soil detection right and wrong are invasive and do not need to detect preceding special enteron aisle preparation, and this greatly reduces patient physiological and psychological discomfort.Although it is clinical that present large bowel cancer detection technique has been widely used in, people still need exploitation to have tumour-specific more, and the detection method of Noninvasive is monitored the variation of gut epithelium tissue.Immunomagnetic beads enrichment fecal cast-off cell detection method is exactly a kind of novel large bowel cancer detection technique that the applications well prospect is arranged.
Research confirms that already gut epithelium is organized always and all upgraded at the very fast cell that carries out, and with 1% Velocity Updating per hour, has nearly 10 every day approximately
10Individual normal epithelium cell comes off, and eugonic tumour epithelial cell renewal speed is faster.Cast-off cells can provide multiple different detection approach for abnormal conditions such as the tumour that detects enteron aisle, polyp, ulcer, and for example detection in Gene Mutation, gene expression amount detect and enzyme activity detection etc.Early detection to cast-off cells is the key factor that malignant tumour takes place and in time treats prophylaxis of tumours.
3/4 is water in the composition of ight soil, and 1/4 is solid; 30% is dead bacterium in the solid, and 10%～20% is fat, and 2%～3% is protein, and 10%～20% is inorganic salt, and 30% is the epithelial cell of solids component as coming off in indigested remaining food and the Digestive system.The key of extracting cast-off cells is that cast-off cells and other compositions of ight soil are separated.
Epithelial cell adhesion molecule EpCAM (Epithelial Cell Adhesion Molecule) is a kind of epithelial cell specific antigens, belong to a type transmembrane glycoprotein, it can be expressed in the cell surface of basolateral membrane in most of epithelium, but is not expressed in for example epithelial cell of liver cell and stomach of sophisticated flat epithelial cell and epithelial cell that some is special.Report that EpCAM expression amount in tumour cell increases, and has new expression in hypogenetic flat epithelial cell.EpCAM has become a kind of important tumour cell marker now.
Monoclonal antibody at EpCAM is Ber-EP4, is a kind of mouse resource monoclonal antibody, obtains with human breast cancer cell line mcf-7 immune balb/c mice.The principle of immunomagnetic beads enrichment large bowel cancer cast-off cells is to utilize the antibody of the goat anti-mouse igg of biotin modification, with finishing the magnetic bead of streptavidin affine the combination taken place, make two anti-bags by in magnetic bead surfaces, reach the fixed purpose.Antigen antibody reaction can take place with Ber-EP4 in the antibody of the goat anti-mouse igg of biotin modification, and Ber-EP4 can combine with the EpCAM of cell surface, thereby cast-off cells are linked to each other with magnetic bead.
Immunomagnetic beads enrichment fecal cast-off cell detection method has pollution-free, accuracy height, characteristics such as specificity is good.But still by operation manually, there is the skilled operation otherness in this mode, and is subjected to the influence of operating speed intensity in the extraction of cast-off cells at present, is not suitable for carrying out the clinical analysis detection of high-throughput, large sample.In addition, because sample comes from ight soil, operation can be subjected to the influence of reviewer's conformability.
Summary of the invention
Purpose of the present invention then is at present above-mentioned the deficiencies in the prior art, a kind of automatization cast-off cells extraction apparatus is provided, realize handling fecal sample automatically and utilize large bowel cancer cast-off cells in the immunomagnetic beads enrichment fecal sample, overcome the skilled operation difference that manual operation exists, defectives such as intensity is not high, and flux is little, and sample size is little, operator need not directly to contact fecal sample, have improved operator's conformability; The false positive rate, the false negative rate that have overcome ight soil occult blood examination existence are all higher, the defective of poor specificity; Overcome the defective of splanchnoscopy experimenter poor compliance.
Technical scheme of the present invention is as follows:
A kind of automatic fecal cast-off cell extracting device, it is characterized in that it includes the sample collecting device, sample processing device, cell enrichment device and assisted automated mechanism thereof, described sample collecting device is made of sampling pipe sealing cover and sampling pipe, on the sampling pipe sealing cover inner side-wall sample scoop is installed, the sampling pipe sealing cover is installed on the sampling pipe upper end, the lower end of sampling pipe links to each other with the sample processing device upper end, the sample processing device lower end mouth of pipe is connected to the cell enrichment device by pipeline, described assisted automated mechanism is divided into sample processing device and is inverted and the power system that resets, sample processing device shakes power system up and down, liquid-flow power system and cell enrichment device subsystem.
The end that described sample processing device links to each other with the sample collecting device is provided with the internal thread that matches with sample collecting device lower port place outer wall thread, this internal thread bottom is provided with a circle inner card cage, the lower port of sample collecting device seals by tinfoil, screw in the process at the sample collecting device, tinfoil will be scratched by inner card cage, makes sample enter sample processing device.Treatment unit top tube wall has inlet opening, links to each other with emulsion tube, can go into damping fluid by infusion.
Described sample processing device is divided into upper, middle, and lower part, by seal with screwed joint, has been placed with two granulated glass spherees of porphyrization in the sample processing device between three parts.
Described sample processing device middle part inwall is fixed with the double layer of metal filter screen, and the filter screen aperture is respectively 500 microns and 200 microns.
Described sample processing device lower base has fluid hole, and this fluid hole is connected with peristaltic pump by emulsion tube, can filtrate be extracted out by peristaltic pump.
Cell enrichment pipe pipe in the described cell enrichment device covers and is provided with three holes, connect three emulsion tubes respectively, wherein two insertion ends are liquid-inlet pipe than what lack, long insertion cell enrichment bottom of device is a drain pipe, is used for that sample filtrate is injected cell enrichment device liquid-inlet pipe and is connected to the sample processing device lower end; Drain pipe links to each other with the waste collection device, is used for waste liquid is extracted out; Liquid-inlet pipe links to each other with the damping fluid liquid storage bottle, is used for fresh buffer is injected the example enrichment device.
Described sample processing device is inverted and the power system that resets adopts the phase place motor, and behind the power connection, phase switch rotates with axle, contact with contact switches when spending when going to 180, the motor outage, axle stops operating, and the sample processing device that link to each other with axle this moment is inversion state; After oppositely connecting power supply, the phase switch backward rotation, after the opposite side contact switches contacts, the motor outage, axle stops operating, and this moment, sample processing device resetted.
Described sample processing device shakes up and down that motor shaft links to each other with cam in the power system, sample processing device is fixed on the Z-axis, this axle links to each other with above-mentioned cam, drives cam when motor rotates and realizes that Z-axis shakes up and down, thereby sample processing device is shaken up and down.
Described liquid-flow power system comprises the peristaltic pump of emulsion tube that connects each port and controlled liq flow direction, speed and the time that is connected with emulsion tube.
Described cell enrichment device subsystem is divided into thermostat and enrichment with magnetic bead device, described thermostat is made up of temperature sensor and electric heater unit, when temperature sensor detected temperature and is lower than set(ting)value, electric heater unit began heating, guaranteed homo(io)thermism.Described enrichment with magnetic bead device includes cell enrichment pipe, magnet sleeve and magnet, and magnet frees in and out in magnet sleeve, and magnet sleeve is positioned at the cell enrichment pipe.
Enrichment with magnetic bead device of the present invention has designed two schemes, and scheme one is that magnet passes through running gear, and after near the enrichment with magnetic bead tube wall when outside magnetic bead being adsorbed in the tube wall inboard, treating that washing is finished, magnet leaves tube wall, and magnetic bead is suspended from the liquid again.Scheme two is to be positioned over magnet sleeve at magnet, and magnet and magnet sleeve can move up and down respectively, and magnet and magnet sleeve stretch into below the enrichment with magnetic bead intraluminal fluid face jointly, magnetic bead is adsorbed in sleeve outer wall, after washing was finished, magnet left, and magnetic bead is suspended from the liquid again.
Beneficial effect of the present invention has:
Automatization cast-off cells extraction equipment will well remedy present defective.Design and the supporting sampler of extraction apparatus seal collected specimens after, deliver to inspection department after, directly send into extraction apparatus, all the other operations are all finished in the extraction apparatus that seals.The cleaning that the use of automatization cast-off cells extraction apparatus will keep inspection department, prevent the infection of pathogenic micro-organism etc., the entire operation process, the reviewer need not contact sample, improved the comfortableness of detecting operation, guarantee to carry out in a large number under the prerequisite of extraction efficiency, cell extraction, check fast.The design of automatization cast-off cells extraction apparatus, application will be accelerated large bowel cancer cast-off cells detection method greatly and be applied to clinical process.
Description of drawings
Fig. 1 is a sample collecting device synoptic diagram.
Fig. 2 is the sample collecting synoptic diagram.
Fig. 3 is sample collecting device and sample processing device synoptic diagram.
Fig. 4 is for sample processing device is inverted, vibration power system synoptic diagram.
Fig. 5 is a phase place motor synoptic diagram.
Fig. 6 is a sample preparation process synoptic diagram.
Fig. 7 is an automatization cast-off cells extraction element general illustration
Fig. 8 is cell enrichment system schematic (scheme one)
Fig. 9 is cell enrichment system schematic (scheme two)
In the accompanying drawing, 1, the sample collecting device; 2, sample collecting device sealing cover; 3, sample scoop; 4, sealing tinfoil; 5, sampling pipe; 6, fecal sample; 7, sample processing device; 8, sample processing device top; 9, sample processing device middle part; 10, sample processing device bottom; 11, inner card cage; 12, the feed liquor mouth of pipe; 13,500 micron screen; 14,200 micron screen; 15, the fluid mouth of pipe; 16, phase place motor; 17, motor; 18, cam; 19, power system is inverted, is vibrated to sample processing device; 20, transverse axis; 21, phase switch; 22, elastic sheet switch; 23, glass marbles; 24, cell enrichment system; 25, peristaltic pump; 26, cell enrichment pipe; 27, electrically heated thermostat; 28, magnet and running gear; 29, liquid-inlet pipe 1; 30, pumping pipe; 31, liquid-inlet pipe 2; 32, magnet sleeve; 33, magnet; 34, the cell enrichment pipe 1; 35, the cell enrichment pipe 2; 36, the cell enrichment pipe 3.
Embodiment
The invention will be further described below in conjunction with the drawings and specific embodiments:
The invention provides an automatic fecal cast-off cell extraction apparatus, be based on following discovery and breakthrough: 1, gut epithelium is organized always all and is upgraded at the very fast cell that carries out, and with 1% Velocity Updating per hour, has nearly 10 every day approximately
10Individual normal epithelium cell comes off, and eugonic tumour epithelial cell renewal speed is faster.2, epithelial cell adhesion molecule EpCAM (EpithelialCell Adhesion Molecule) is a kind of epithelial tumor cell mark antigen, can be combined by the specific antibody in magnetic bead surfaces with its bag.3, the magnetic microsphere realization enrichment that under the action of a magnetic field, can flock together.
After using sample collecting device of the present invention to collect faecal samples, the sample collecting device is inserted treater, through diluting, shake, pulverizing, ight soil is by mixing, and the filter screen of flowing through is removed macrobead and fiber, contains cast-off cells in the remaining liq.Collect filtrate and immunomagnetic beads temperature and bathe, magnetic bead is combined with cast-off cells, obtain purified cell-magnetic bead mixture by the enrichment with magnetic bead technology.
The specific design scheme is as follows:
1. the design of two screw threads:
As shown in Figure 1, sample collecting device 1 uses the structure design of the two screw threads in two ends, one end and sample collecting device sealing cover 2 screw and reach sealing, and the other end can screw with sample processing device sample processing device top 8 and reach sealing, and the lower end of sample collecting device 1 seals with sealing tinfoil 4.
2. the take a sample design of little spoon:
There is a sample scoop 3 center of sample collecting device sealing cover 2 inboards, can once or repeatedly gather fecal sample as required.
3. the screw thread of sample processing apparatus design:
As shown in Figure 3, the sealing of sample processing apparatus 7 realizes that by screw thread the connection of its three inter-modules all has the respective threads design.
4. the design of glass marbles:
Put into glass marbles on sample processing apparatus 8 tops, glass marbles can play the effect of smashing ight soil along with the instrument vertical tremor in the vibrations process.
5. the design of double-level-metal filter screen:
The middle portion at sample processing apparatus middle part 9 has the double layer of metal filter screen, its aperture be respectively 500um and 200um, be used for filtering the macrobead and the fiber of ight soil.
6. the circuit design of peristaltic pump feed liquor and drawing liquid:
Realize the process of feed liquor and drawing liquid by peristaltic pump.
In 10 lower ends, sample processing device bottom a pipeline is arranged, emulsion tube is connected in the bottom of sample product treater and links to each other with peristaltic pump, by drawing liquid filtrate is pumped in the cell enrichment device.
As shown in Figure 8, three pipelines are arranged in the cell enrichment pipe 26 of cell enrichment device, wherein two is the feed liquor pipeline, and a drawing liquid pipeline, the emulsion tube of feed liquor pipeline had just been inserted cell enrichment pipe 26 lids, at the bottom of the direct tubular stinger of the emulsion tube of drawing liquid.Three pipelines all link to each other with peristaltic pump and realize the power of liquid-flow.
7. utilize the design of enrichment with magnetic bead cast-off cells:
By connect in magnetic bead surfaces can with cast-off cells bonded compound antibody, magnetic bead can specificly be combined with cast-off cells, by cleaning repeatedly after the combination, final enrichment obtains single magnetic bead antigen-antibody complex that is connected with cast-off cells.
8. the design of thermostat container:
Electricity cushioning constant temperature system 27 is made up of electric heater unit and temperature sensor, and when transmitter detected that temperature is lower than set(ting)value in the cell enrichment pipe 26, electric heater unit was started working, assurance temperature constant.
9. the design that moves horizontally of magnet:
When magnetic bead is finished the concussion incubation in cell enrichment pipe 26 after, moving horizontally magnet is advanced to it near tube wall of cell enrichment pipe 26, at this moment, the magnetic bead immune complex that combines cast-off cells is enriched in tube wall, extracts remaining liquid out by pumping pipe 30 then.
Before feed liquor next time, move horizontally magnet, make the tube wall of magnet away from cell enrichment pipe 26.At this moment, the effect that magnetic bead is no longer magnetic field force induced and accumulate in tube wall is opened oscillator it is slowly mixed.
10. the design that moves horizontally of the design of magnet VTOL (vertical take off and landing) and base:
When magnetic bead is finished the concussion incubation in the cell enrichment pipe after, the magnet and the sleeve pipe of cell enrichment pipe top are vertically moved down the insertion liquid internal, at this moment, the magnetic bead immune complex that combines cast-off cells is enriched in sleeve outer wall, extracts remaining liquid out by the drawing liquid mouth of pipe then.
Before feed liquor next time, pan-up magnet 33 and magnet sleeve 32.At this moment, magnetic bead still accumulates in the cover tube wall, and horizontal base moves forward, and takes advantage of the pipe of fresh washing lotion to move to the bottom of magnet another.
Operating process of the present invention is as follows:
1. as shown in Figure 2, get about the about 3g of fresh ight soil to be checked (can take a sample several times according to practical situation) with sample scoop 3, sampling pipe 5 is inserted in the sampling back, screws secluding air, and 4 ℃ of preservations, and haulage time is no more than 2 hours.
2. sample processing device top 8 places two granulated glass spherees in advance, has an end of tinfoil to insert sample processing device sample collecting device 1, screws, and the sealing tinfoil 4 that is enclosed in this side can be scratched.
3. sample processing device 7 automatic 180 degree are inverted (as shown in Figure 6).
4. inject damping fluid by liquid-inlet pipe automatically after inversion is finished, damping fluid produces diluting effect to ight soil in the sample processing device 7.
5. the automatic up-down vibration of sample processing device makes the glass marbles of putting in advance that ight soil is smashed and mixing.
6. after treating that concussion is pulverized, treater 180 degree once more is inverted, and returns back to original state.
7. blended liquid is pumped in the cell enrichment pipe 26 in the cell enrichment device (as shown in Figure 7) by pumping pipe through 500 microns and 200 micron screen successively.
Cast-off cells combine with immunomagnetic beads and washing design two schemes (shown in Fig. 8,9):
Scheme one (as shown in Figure 8):
8 (a). put into an amount of magnetic bead in the cell enrichment pipe 26 in advance, and the temperature of thermostat container is adjusted to 37 ℃, after the filtrate for the treatment of ight soil is injected collection device fully, mix concussion, incubation is 30 minutes under this temperature.
9 (a). the magnet automatic horizontal is advanced to the tube wall place of cell enrichment pipe 26, and this moment, magnetic bead was enriched in the tube wall inboard.
10 (a). open pumping pipe remaining liquid in the cell enrichment pipe 26 is extracted out.
11 (a) magnet, 33 levels oppositely move, and leave cell enrichment pipe 26 tube wall places.
12 (a) open liquid-inlet pipe, inject fresh damping fluid to cell enrichment pipe 26.
After 13 (a) treat that damping fluid flows into cell enrichment pipe 26 fully, mix concussion, magnetic bead is suspended from the damping fluid again.
14 (a). magnet automatic horizontal once more moves to the tube wall place of cell enrichment pipe 26, and this moment, magnetic bead was enriched in the tube wall inboard.
15 (a). open pumping pipe liquid in the cell enrichment pipe is extracted out.
16 (a). repeat 5-9 and go on foot twice.
17 (a). take out the cell enrichment pipe in the cell enrichment device, and add an amount of damping fluid to it, purified cast-off cells-magnetic bead mixture is suspended from the damping fluid.
Scheme two (as shown in Figure 9):
8 (b). put into an amount of magnetic bead in the cell enrichment pipe 26 in advance, and the temperature of thermostat container is adjusted to 37 ℃, after the filtrate for the treatment of ight soil is injected cell enrichment pipe 26 fully, mix concussion, incubation is 30 minutes under this temperature.
9 (b). magnet 33 and magnet sleeve 32 move down automatically, are inserted into liquid internal in the cell enrichment pipe 26, make enrichment with magnetic bead on magnet sleeve 32.
10 (b). magnet 33 and magnet sleeve 32 move up automatically, and base moves forward to the position of next enrichment pipe automatically.
11 (b). magnet 33 and magnet sleeve 32 move down automatically, are inserted into liquid internal, and this moment, enrichment with magnetic bead was on magnet sleeve 32.
12 (b). magnet 33 moves up automatically, and magnet sleeve 32 is still stayed in the enrichment pipe, mixes concussion, and magnetic bead is suspended from the damping fluid again.
13 (b). magnet is moved down in the sleeve pipe automatically, makes in the damping fluid enrichment with magnetic bead on magnet sleeve.
14 (b). magnet and magnet sleeve move up automatically, and base moves forward to the position of next enrichment pipe automatically.
15 (b). repeat the 4-6 step once.
16 (b). magnet and magnet sleeve move up automatically, and magnet and magnet sleeve are moved in the enrichment pipe that appropriate amount of buffer solution is housed, and remove magnet, and suitably concussion is suspended from the middle of the damping fluid purified cast-off cells-magnetic bead mixture.
Claims (10)
1. automatic fecal cast-off cell extracting device, it is characterized in that it includes the sample collecting device, sample processing device, cell enrichment device and assisted automated mechanism thereof, described sample collecting device is made of sampling pipe sealing cover and sampling pipe, on the sampling pipe sealing cover inner side-wall sample scoop is installed, the sampling pipe sealing cover is installed on the sampling pipe upper end, the lower end of sampling pipe links to each other with the sample processing device upper end, the sample processing device lower end mouth of pipe is connected to the cell enrichment device by pipeline, described assisted automated mechanism is divided into sample processing device and is inverted and the power system that resets, sample processing device shakes power system up and down, liquid-flow power system and cell enrichment device subsystem.
2. automatic fecal cast-off cell extracting device according to claim 1, it is characterized in that the end that described sample processing device links to each other with the sample collecting device is provided with the internal thread that matches with sample collecting device lower port place outer wall thread, this internal thread bottom is provided with a circle inner card cage, the lower port of sample collecting device seals by tinfoil, and sample processing device top tube wall has inlet opening.
3. automatic fecal cast-off cell extracting device according to claim 1 and 2, it is characterized in that described sample processing device is divided into upper, middle, and lower part, by seal with screwed joint, two granulated glass spherees of porphyrization have been placed with in the sample processing device between three parts.
4. automatic fecal cast-off cell extracting device according to claim 3 is characterized in that described sample processing device middle part inwall is fixed with the double layer of metal filter screen, and the filter screen aperture is respectively 500 microns and 200 microns.
5. automatic fecal cast-off cell extracting device according to claim 1 is characterized in that described sample processing device lower base has fluid hole, and this fluid hole is connected with peristaltic pump by emulsion tube.
6. automatic fecal cast-off cell extracting device according to claim 1, it is characterized in that cell enrichment pipe pipe in the described cell enrichment device covers is provided with three holes, connect three emulsion tubes respectively, wherein two insertion ends are liquid-inlet pipe than what lack, long insertion cell enrichment bottom of device is a drain pipe, liquid-inlet pipe is connected to the sample processing device lower end, and drain pipe links to each other with the waste collection device, and liquid-inlet pipe links to each other with the damping fluid liquid storage bottle.
7. automatic fecal cast-off cell extracting device according to claim 1 is characterized in that described sample processing device is inverted and the power system that resets adopts the phase place motor.
8. automatic fecal cast-off cell extracting device according to claim 1 is characterized in that described sample processing device shakes up and down that motor shaft links to each other with cam in the power system, and sample processing device is fixed on the Z-axis, and this axle links to each other with above-mentioned cam.
9. automatic fecal cast-off cell extracting device according to claim 1 is characterized in that described liquid-flow power system comprises the peristaltic pump of emulsion tube that connects each port and controlled liq flow direction, speed and the time that is connected with emulsion tube.
10. automatic fecal cast-off cell extracting device according to claim 1, it is characterized in that described cell enrichment device subsystem is divided into thermostat and enrichment with magnetic bead device, described thermostat is made up of temperature sensor and electric heater unit, described enrichment with magnetic bead device includes cell enrichment pipe, magnet sleeve and magnet, magnet frees in and out in magnet sleeve, and magnet sleeve is positioned at the cell enrichment pipe.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910225360 CN101701186B (en) | 2008-11-25 | 2009-11-17 | Automatic fecal cast-off cell extracting device |
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| CN200810236308.0 | 2008-11-25 | ||
| CN200810236308 | 2008-11-25 | ||
| CN 200910225360 CN101701186B (en) | 2008-11-25 | 2009-11-17 | Automatic fecal cast-off cell extracting device |
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| CN101701186A true CN101701186A (en) | 2010-05-05 |
| CN101701186B CN101701186B (en) | 2012-07-25 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107702947A (en) * | 2016-08-31 | 2018-02-16 | 孟素兰 | A kind of collecting dung structure |
| CN108844861A (en) * | 2018-07-10 | 2018-11-20 | 张素红 | A kind of asphalt cold repairing low temperature fluidity test device |
| CN109060494A (en) * | 2018-08-25 | 2018-12-21 | 王明芳 | Pretreatment unit for oncology immunomagnetic beads enrichment fecal cast-off cell |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6521190B1 (en) * | 2000-05-19 | 2003-02-18 | Digene Corporation | Cell collection apparatus |
| CN2799572Y (en) * | 2005-06-16 | 2006-07-26 | 姜世民 | Automatic separation and culturing device for biological sample |
| CN201072391Y (en) * | 2007-08-06 | 2008-06-11 | 浙江大学 | Automatic objective separation device based on immune magnetic beads |
| RU2010127266A (en) * | 2007-12-05 | 2012-01-10 | Зиомикс, Инк. (Us) | CELL ANALYSIS KIT AND METHOD |
| CN201567330U (en) * | 2008-11-25 | 2010-09-01 | 周国华 | Automated excrement cast-off cell extracting device |
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2009
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107702947A (en) * | 2016-08-31 | 2018-02-16 | 孟素兰 | A kind of collecting dung structure |
| CN108844861A (en) * | 2018-07-10 | 2018-11-20 | 张素红 | A kind of asphalt cold repairing low temperature fluidity test device |
| CN109060494A (en) * | 2018-08-25 | 2018-12-21 | 王明芳 | Pretreatment unit for oncology immunomagnetic beads enrichment fecal cast-off cell |
| CN109060494B (en) * | 2018-08-25 | 2021-03-16 | 王明芳 | A preprocessing device that is used for oncology immunomagnetic bead enrichment excrement and urine to drop cell |
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| CN101701186B (en) | 2012-07-25 |
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