CN101700059A - Tremellodon gelatinosum mycelium preservative and preparation method thereof - Google Patents
Tremellodon gelatinosum mycelium preservative and preparation method thereof Download PDFInfo
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- CN101700059A CN101700059A CN200910095191A CN200910095191A CN101700059A CN 101700059 A CN101700059 A CN 101700059A CN 200910095191 A CN200910095191 A CN 200910095191A CN 200910095191 A CN200910095191 A CN 200910095191A CN 101700059 A CN101700059 A CN 101700059A
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- 230000002335 preservative effect Effects 0.000 title claims abstract description 9
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- Storage Of Fruits Or Vegetables (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention provides a tremellodon gelatinosum mycelium preservative and a preparation method thereof. The production technology procedure is as follows: drying the tremellodon gelatinosum mycelium, smashing, ultrasonically processing, processing by means of enzymolysis and filtering to obtain the extracting solution of the tremellodon gelatinosum mycelium; and allocating with agar, carboxymethyl chitosan, citric acid and sodium citrate to obtain the tremellodon gelatinosum mycelium preservative. The tremellodon gelatinosum mycelium preservative prepared by the method is a natural food preservative which is mainly used for the coating preservation of high-quality wild edible mushrooms such as tricholoma matsutake, toadstool and termite mushroom, etc. The invention can prolong the preservation time at 6-9 days, thereby being capable of reducing the deterioration degree when storing and transferring the high-quality wild edible mushrooms and improving the commodity value.
Description
Technical field
The present invention relates to the food preservative technology field, specifically a kind of Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium anti-staling agent and preparation method thereof.
Background technology
Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb (Pseudohydnum gelatinosum) is the soft colloid of basidiocarps, is famous and precious wild edible fungus.Measure Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb according to one's analysis and contain abundant polysaccharide, protein, fat, carbohydrate, crude fibre, phosphorus, iron, calcium, riboflavin, carrotene, 17 seed amino acids and trace element, the ergosterol and the more complete vitamin of kind that also contain general vegetables and lacked.The flat flavor of Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb is sweet, wind-evil dispelling and cold-evil expelling is arranged, reduce the effect of cholesterol, and the effect of relaxing the muscles and stimulate the blood circulation is arranged, beneficial gas tonifying Qi, control the broken blood of wind and stomach defaecation, hypotensive, separate the poison of food, control effect such as headache and dizziness.Interrelated data shows: the nutrition of edible mushroom mycelium and the content of bioactive ingredients are close with its fructification, have in addition be higher than its fructification.In addition, Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract has good bacteriostasis, and particularly the inhibition effect to mould is more remarkable.
High-grade wild edible fungus water content height such as matsutake, hickory chick, chicken fir are organized very delicacy, storage at normal temperatures after adopting, and the moisture in the mushroom body will evaporate lost in a large number, makes its atrophy, brown stain even rotten, and commodity value descends even loses., bring enormous economic loss will for the mushroom farming in case thereby mushroom causes quality decline not reach outlet and processing request because of causing after adopting to overstock.Therefore, resolving the post-harvest fresh-keeping problem of bright mushroom, prolong its transportation and shelf-life, is the particularly high-grade extremely urgent major issue of mushroom industrialized development of eating raw of pestering.
But because many antistaling agents are chemical synthetic drugs, not only contaminated environment but also can produce potential hazard to consumer's health, long-term use continuously also can make and cause rotten pathogen and develop immunity to drugs, therefore exploring the anticorrosion new technology that can replace existing chemical bactericide just becomes the current new problem that faces of postharvest fruit and vegetable processing researcher, and people more and more tend to select natural materials as food preservative freshness retaining agent.Therefore, adopt harmless and material such as Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium, carry out fresh-keeping, significant high-grade wild edible fungus such as matsutake, hickory chick, chicken firs as antistaling agent with nutritive value.The report that does not have Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium anti-staling agent and preparation method thereof now in the technology.
Summary of the invention
The objective of the invention is in order further to excavate the value of this wild edible fungus resource, providing a kind of is the antistaling agent of primary raw material with the Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium; Another object of the present invention provides the preparation method of this antistaling agent.
The objective of the invention is to be achieved through the following technical solutions:
Raw materials used and the consumption (percentage by weight) of Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium anti-staling agent:
Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract 20%～40%; Agar 0.4%～0.8%; CMC 0.5%～3%; Citric acid 0.5%～1.2%; Natrium citricum 0.08%～0.2%; All the other are water.
The percentage by weight of used culture medium material of described Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium and consumption thereof is: maltose 3%～8%, corn flour 1%～3%, wheat bran 2%～5%, MgSO
40.05%～0.3%, KH
2PO
40.06%～0.2%, ZnSO
40.6%～1.4%, VB
10.01%～0.1%, NAA (NAA) 1～10mg/L, right-chlorophenoxyacetic acid (4-CPA) 1～10mg/L, surplus are water, in above-mentioned culture medium, insert the female kind of Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb, cultivated 7～14 days at 23 ℃ of following shaking tables, getting mycelium, dry the back pulverizing in 60 ℃ of baking ovens standby.
Described Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract is with drying, Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium after the pulverizing steeps with the water logging of equivalent weight, moisturizing to feed liquid weight ratio is 1: 20～1: 40 after ultrasonic wave is handled 15～60 minutes, heat temperature raising to 40～60 ℃, adjust pH to 4～6, the cellulase that adds leaching liquor weight 0.5～2%, enzymatic reaction 30～60min, the papain that adds leaching liquor weight 0.5～2% again, behind enzymatic reaction 30～60min, be warming up to 80～100 ℃ of enzymes and be incubated lixiviate 0.5～2 hour of going out, filter the back decompression and be concentrated to 1/4～1/6 of original volume, promptly get Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract.
This Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium anti-staling agent preparation method may further comprise the steps successively:
A. select materials: select the female kind of vigorous, full, the free of contamination Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb of growth for use;
B. cultivate: the percentage by weight of culture medium material and consumption thereof is: maltose 3%～8%, corn flour 1%～3%, wheat bran 2%～5%, MgSO
40.05%～0.3%, KH
2PO
40.06%～0.2%, ZnSO
40.6%～1.4%, VB
10.01%～0.1%, NAA (NAA) 1～10mg/L, right-chlorophenoxyacetic acid (4-CPA) 1～10mg/L, surplus are water, in above-mentioned culture medium, insert the female kind of Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb, cultivated 7～14 days at 23 ℃ of following shaking tables, getting mycelium, dry the back pulverizing in 60 ℃ of baking ovens standby;
C. lixiviate: the Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium is steeped with the water logging of equivalent weight earlier, ultrasonic wave with 100～420w carries out pre-treatment again, 15～60 minutes processing times, Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium moisturizing to the feed liquid weight ratio that to handle again is to put into the lixiviate jar after 1: 20～1: 40, heat temperature raising to 40～60 ℃, adjust pH to 4～6, the cellulase that adds leaching liquor weight 0.5～2%, enzymatic reaction 30～60min, the papain that adds leaching liquor weight 0.5～2% again, behind enzymatic reaction 30～60min, be warming up to 80～100 ℃ and be incubated lixiviate 0.5～2 hour;
D. filtering and concentrating: cross leaching juice, its filter residue adds the water of 10～30 times of quality, with above-mentioned similarity condition lixiviate, crosses leaching juice afterwards, twice filtrate is merged the back decompression be concentrated to 1/4～1/6 of original volume, is Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract;
E. allotment: the percentage by weight of allotment material and consumption thereof is: Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract 20%～40%, agar 0.4%～0.8%, CMC 0.5%～3%, citric acid 0.5%～1.2%, natrium citricum 0.08%～0.2%, all the other are water; Above-mentioned material is carried out mixing preparation, promptly get the antistaling agent finished product;
F. coating-film fresh-keeping: will treat that fresh-keeping mushroom immerses in the prepared preservative, and after 30 seconds, take out approximately that air-dry with cold wind, it is fresh-keeping to treat can carry out after the tunicle drying on surface room temperature storage.
The invention provides a kind of is that primary raw material prepares antistaling agent and preparation method thereof with the Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium.Because of this product is mainly used in the back coating-film fresh-keeping of adopting of high-grade wild edible fungus such as matsutake, hickory chick, chicken fir, its freshness date can be extended to 6～9 days, thereby can reduce the degree that these high-grade wild edible fungus go bad greatly during storing and transporting, improve its commodity value.
Specific implementation method
Further set forth content of the present invention below in conjunction with embodiment.But be not limitation of the invention.
Embodiment 1:
Select the female kind of vigorous, full, the free of contamination Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb of growth for use, the percentage by weight of culture medium material and consumption thereof is: maltose 8%, corn flour 2%, wheat bran 2%, MgSO
40.3%, KH
2PO
40.2%, ZnSO
41.0%, VB
10.1%, NAA (NAA) 1.5mg/L, right-chlorophenoxyacetic acid (4-CPA) 9.5mg/L, surplus are water, and in above-mentioned culture medium, the access Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb is female plants, and cultivates 14 days at 23 ℃ of following shaking tables, gets mycelium and dry the back pulverizing in 60 ℃ of baking ovens.After the Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium that obtains being used the water logging bubble of equivalent weight earlier, ultrasonic wave with 220w carries out pre-treatment, and in 20 minutes processing times, Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium moisturizing to the feed liquid weight ratio that will handle again is 1: 20, put into the lixiviate jar then, heat temperature raising to 60 ℃, adjust pH to 5 adds the cellulase of leaching liquor weight 0.5%, enzymatic reaction 30min, the papain that adds leaching liquor weight 2% again behind the enzymatic reaction 40min, is warming up to 80 ℃ and be incubated lixiviate 2 hours.Cross leaching juice, its filter residue adds the water of 30 times of quality, with above-mentioned similarity condition lixiviate, crosses leaching juice afterwards, twice filtrate is merged the back decompression be concentrated to 1/6 of original volume, is Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract.By weight percentage, with Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract 30%, agar 0.8%, CMC 3%, citric acid 1.1%, natrium citricum 0.2%, all the other are water, carry out mixing preparation, promptly get the antistaling agent finished product.To treat that fresh-keeping mushroom immerses in the prepared preservative, after 30 seconds, take out approximately that air-dry with cold wind, it is fresh-keeping to treat can carry out after the tunicle drying on surface room temperature storage.
Embodiment 2:
Select the female kind of vigorous, full, the free of contamination Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb of growth for use, the percentage by weight of culture medium material and consumption thereof is: maltose 6%, corn flour 1%, wheat bran 3%, MgSO
40.15%, KH
2PO
40.1%, ZnSO
41.4%, VB
10.05%, NAA (NAA) 9mg/L, right-chlorophenoxyacetic acid (4-CPA) 1.5mg/L, surplus are water, and in above-mentioned culture medium, the access Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb is female plants, and cultivates 8 days at 23 ℃ of following shaking tables, gets mycelium and dry the back pulverizing in 60 ℃ of baking ovens.After the Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium that obtains being used the water logging bubble of equivalent weight earlier, ultrasonic wave with 80w carries out pre-treatment, and in 40 minutes processing times, Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium moisturizing to the feed liquid weight ratio that will handle again is 1: 30, put into the lixiviate jar then, heat temperature raising to 60 ℃, adjust pH to 5.5 adds the cellulase of leaching liquor weight 1%, enzymatic reaction 40min, the papain that adds leaching liquor weight 1% again behind the enzymatic reaction 40min, is warming up to 100 ℃ and be incubated lixiviate 2 hours.Cross leaching juice, its filter residue adds the water of 20 times of quality, with above-mentioned similarity condition lixiviate, crosses leaching juice afterwards, twice filtrate is merged the back decompression be concentrated to 1/4 of original volume, is Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract.By weight percentage, with Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract 40%, agar 0.6%, CMC 2%, citric acid 0.6%, natrium citricum 0.1%, all the other are water, carry out mixing preparation, promptly get the antistaling agent finished product.To treat that fresh-keeping mushroom immerses in the prepared preservative, after 30 seconds, take out approximately that air-dry with cold wind, it is fresh-keeping to treat can carry out after the tunicle drying on surface room temperature storage.
Embodiment 3:
Select the female kind of vigorous, full, the free of contamination Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb of growth for use, the percentage by weight of culture medium material and consumption thereof is: maltose 3.5%, corn flour 3%, wheat bran 5%, MgSO
40.06%, KH
2PO
40.07%, ZnSO
40.7%, VB
10.02%, NAA (NAA) 5mg/L, right-chlorophenoxyacetic acid (4-CPA) 5.5mg/L, surplus are water, and in above-mentioned culture medium, the access Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb is female plants, and cultivates 11 days at 23 ℃ of following shaking tables, gets mycelium and dry the back pulverizing in 60 ℃ of baking ovens.After the Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium that obtains being used the water logging bubble of equivalent weight earlier, ultrasonic wave with 420w carries out pre-treatment, and in 30 minutes processing times, Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium moisturizing to the feed liquid weight ratio that will handle again is 1: 40, put into the lixiviate jar then, heat temperature raising to 45 ℃, adjust pH to 5.5 adds the cellulase of leaching liquor weight 2%, enzymatic reaction 60min, the papain that adds leaching liquor weight 0.5% again behind the enzymatic reaction 60min, is warming up to 100 ℃ and be incubated lixiviate 2 hours.Cross leaching juice, its filter residue adds the water of 10 times of quality, with above-mentioned similarity condition lixiviate, crosses leaching juice afterwards, twice filtrate is merged the back decompression be concentrated to 1/5 of original volume, is Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract.By weight percentage, with Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract 20%, agar 0.4%, CMC 0.8%, citric acid 1.2%, natrium citricum 0.15%, all the other are water, carry out mixing preparation, promptly get the antistaling agent finished product.To treat that fresh-keeping mushroom immerses in the prepared preservative, after 30 seconds, take out approximately that air-dry with cold wind, it is fresh-keeping to treat can carry out after the tunicle drying on surface room temperature storage.
Claims (3)
1. an Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium anti-staling agent is characterized in that this Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium anti-staling agent percentage by weight raw materials used and consumption is: Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract 20%～40%; Agar 0.4%～0.8%; CMC 0.5%～3%; Citric acid 0.5%～1.2%; Natrium citricum 0.08%～0.2%; All the other are water; Described Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract is with drying, Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium after the pulverizing steeps with the water logging of equivalent weight, moisturizing to feed liquid weight ratio is 1: 20～1: 40 after ultrasonic wave is handled 15～60 minutes, heat temperature raising to 40～60 ℃, adjust pH to 4～6, the cellulase that adds leaching liquor weight 0.5～2%, enzymatic reaction 30～60min, the papain that adds leaching liquor weight 0.5～2% again, behind enzymatic reaction 30～60min, be warming up to 80～100 ℃ of enzymes and be incubated lixiviate 0.5～2 hour of going out, filter the back decompression and be concentrated to 1/4～1/6 of original volume, promptly get Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract.
2. the preparation method of the described Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium anti-staling agent of claim 1 is characterized in that carrying out according to the following steps:
A. the mycelial cultivation of Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb: the percentage by weight of culture medium material and consumption thereof is: maltose 3%～8%, corn flour 1%～3%, wheat bran 2%～5%, MgSO
40.05%～0.3%, KH
2PO
40.06%～0.2%, ZnSO
40.6%～1.4%, VB
10.01%～0.1%, NAA (NAA) 1～10mg/L, right-chlorophenoxyacetic acid (4-CPA) 1～10mg/L, surplus are water, in above-mentioned culture medium, insert the female kind of Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb, cultivated 7～14 days at 23 ℃ of following shaking tables, getting mycelium, dry the back pulverizing in 60 ℃ of baking ovens standby;
B. lixiviate: the Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium is steeped with the water logging of equivalent weight earlier, ultrasonic wave with 100～420w carries out pre-treatment again, 15～60 minutes processing times, Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium moisturizing to the feed liquid weight ratio that to handle again is to put into the lixiviate jar after 1: 20～1: 40, heat temperature raising to 40～60 ℃, adjust pH to 4～6, the cellulase that adds leaching liquor weight 0.5～2%, enzymatic reaction 30～60min, the papain that adds leaching liquor weight 0.5～2% again, behind enzymatic reaction 30～60min, be warming up to 80～100 ℃ and be incubated lixiviate 0.5～2 hour;
C. filtering and concentrating: cross leaching juice, its filter residue adds the water of 10～30 times of weight, with above-mentioned similarity condition lixiviate, crosses leaching juice afterwards, twice filtrate is merged the back decompression be concentrated to 1/4～1/6 of original volume, is Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract;
D. allotment: the percentage by weight of allotment material and consumption thereof is: Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium extract 20%～40%, agar 0.4%～0.8%, CMC 0.5%～3%, citric acid 0.5%～1.2%, natrium citricum 0.08%～0.2%, all the other are water, above-mentioned material is carried out mixing preparation, promptly get the antistaling agent finished product.
3. the using method of the described Albatrellus dispansus (Lloyd) Canf. ﹠ Gilb mycelium anti-staling agent of claim 1 is characterized in that treating that fresh-keeping mushroom immerses in the prepared preservative, takes out after 30 seconds approximately, and is air-dry with cold wind, and it is fresh-keeping to treat can carry out after the tunicle drying on surface room temperature storage.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104855141A (en) * | 2015-06-11 | 2015-08-26 | 宁德师范学院 | Edible mushroom liquid strain preparation method based on ultrasonic crushing |
| CN105410167A (en) * | 2015-11-25 | 2016-03-23 | 云南云菌科技（集团）有限公司 | Edible mushroom fresh keeping agent and preparation method thereof |
| CN106578012A (en) * | 2016-11-15 | 2017-04-26 | 东莞东阳光药物研发有限公司 | Fresh keeping agent for morchella and application thereof |
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2009
- 2009-11-13 CN CN200910095191A patent/CN101700059A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104855141A (en) * | 2015-06-11 | 2015-08-26 | 宁德师范学院 | Edible mushroom liquid strain preparation method based on ultrasonic crushing |
| CN104855141B (en) * | 2015-06-11 | 2017-05-17 | 宁德师范学院 | Edible mushroom liquid strain preparation method based on ultrasonic crushing |
| CN105410167A (en) * | 2015-11-25 | 2016-03-23 | 云南云菌科技（集团）有限公司 | Edible mushroom fresh keeping agent and preparation method thereof |
| CN106578012A (en) * | 2016-11-15 | 2017-04-26 | 东莞东阳光药物研发有限公司 | Fresh keeping agent for morchella and application thereof |
| CN106578012B (en) * | 2016-11-15 | 2020-04-21 | 东莞东阳光保健品研发有限公司 | Morchella preservative and application thereof |
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