CN101691348B - Method for extracting astaxanthin from haematococcus pluvialis - Google Patents
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- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 title claims abstract description 50
- 235000013793 astaxanthin Nutrition 0.000 title claims abstract description 50
- 239000001168 astaxanthin Substances 0.000 title claims abstract description 50
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 title claims abstract description 50
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 2
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- 235000006708 antioxidants Nutrition 0.000 description 2
- RASZIXQTZOARSV-BDPUVYQTSA-N astacin Chemical compound CC=1C(=O)C(=O)CC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)C(=O)CC1(C)C RASZIXQTZOARSV-BDPUVYQTSA-N 0.000 description 2
- 235000013734 beta-carotene Nutrition 0.000 description 2
- 239000011648 beta-carotene Substances 0.000 description 2
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 2
- 229960002747 betacarotene Drugs 0.000 description 2
- FDSDTBUPSURDBL-LOFNIBRQSA-N canthaxanthin Chemical compound CC=1C(=O)CCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)CCC1(C)C FDSDTBUPSURDBL-LOFNIBRQSA-N 0.000 description 2
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- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 2
- 102000034498 Astacin Human genes 0.000 description 1
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- 241000894006 Bacteria Species 0.000 description 1
- FMKGDHLSXFDSOU-BDPUVYQTSA-N Dienon-Astacin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)C(=O)C(=CC1(C)C)O)C=CC=C(/C)C=CC2=C(C)C(=O)C(=CC2(C)C)O FMKGDHLSXFDSOU-BDPUVYQTSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
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- 108090000790 Enzymes Proteins 0.000 description 1
- 241000168525 Haematococcus Species 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- OOUTWVMJGMVRQF-DOYZGLONSA-N Phoenicoxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)C(=O)C(O)CC1(C)C)C=CC=C(/C)C=CC2=C(C)C(=O)CCC2(C)C OOUTWVMJGMVRQF-DOYZGLONSA-N 0.000 description 1
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
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- 238000009825 accumulation Methods 0.000 description 1
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- 150000001514 astaxanthins Chemical class 0.000 description 1
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The invention provides a method for extracting astaxanthin from haematococcus pluvialis. Astaxanthin is extracted by adopting above-critical CO2 extracting process. The temperature of an extracting kettle is ranged from 30 to 50 DEG C and the pressure is ranged from 20 to 30 MPa. Flowing speed of CO2 fluid is 20 to 40 L/h and the extracting time is 2 to 4 h. The astaxanthin has strongest inoxidizability and is easy to be oxidized. The extracting method of the invention is simple, rapid and convenient to operate. CO2 is used as the extraction solvent in extraction process, so the production process is environment-friendly and the extraction has no organic solvent residue. The temperature during extraction process is low for efficiently avoiding active substance to decompose at high temperature. The acquired astaxanthin extraction is bolarious pasty substance and can be used as materials for developing health-care food or officinal component.
Description
Technical field
The invention belongs to extraction and the working method of natural product, relate generally to a kind of from Haematocoocus Pluvialls the method for supercritical extraction astaxanthin.
Background technology
Astaxanthin (being called again astacin) is a kind of haematochrome, and its chemical structure is similar to β-carotene.Astaxanthin is a kind of of carotenoids group, is also the synthetic highest level product of class Hu Luosu.β-carotene, xenthophylls, canthaxanthin, Lyeopene etc. are only all the intermediate products of carotenogenesis, and therefore at nature, astaxanthin has the strongest oxidation-resistance.Research finds, astaxanthin has important physiological function, and its resistance of oxidation is stronger, is 550 times of vitamin-E, is 10 times of β-carotenoid etc., can the peroxidation of more effective prevention unsaturated fatty acids.In addition, astaxanthin also has very strong inhibition tumor growth, strengthens immunologic function, resists the multiple physiological functions such as uv damage, thereby is widely used in the fields such as food, medicine, healthcare products and makeup.
Due to its powerful oxidation-resistance, the makeup brand of the similar line in the whole world has all been added the composition of natural astaxanthin as its superpower antioxidant, especially the brand Kosé of Japan, virtue special astaxanthin series moisturizer, eye cream, facial mask, the lipstick etc. released such as triumphant.The Health Products Enterprises in the whole world has released also that about astaxanthin of money more than 200 is soft, the protective foods of hard capsule, oral liquid.In Japan, over nearly 3 years, astaxanthin becomes one of the most fiery heath food.
The source of astaxanthin comprises two kinds of chemosynthesis and biological extraction.The synthetic technology of astaxanthin is very ripe, has reached the degree of suitability for industrialized production.But the synthetic astaxanthin is not only expensive, and with natural astaxanthin at aspect significant differences such as structure, function, application and securities.The nature astaxanthin is by algae, bacterium and plant plankton deposits yields.And in the biogenetic derivation of natural astaxanthin, the content astaxanthin of Haematocoocus Pluvialls is the highest, be acknowledged as occurring in nature and produce the best biology of natural astaxanthin, therefore, utilize this little algae to extract astaxanthin and have undoubtedly vast potential for future development, become in recent years the study hotspot of natural astaxanthin production in the world.
At present, the technology that the overseas utilization Haematocoocus Pluvialls extracts natural astaxanthin has obtained tremendous development, and company as existing in Sweden adopts the bioreactor of totally enclosed (artificial source's irradiation) to produce astaxanthin; The Aquasearch company of the U.S. carries out Haematocoocus Pluvialls and cultivates in computer-controlled outdoor closed photobioreactor (AGM); The little algae of U.S. Cyanotech company adopts " platform " formula bioreactor of sealing to carry out the cultivation of Haematocoocus Pluvialls.In recent years, domesticly also making some progress aspect astaxanthin research, patent CN1392244A has studied a kind of method of cultivating haematococcus pluvialis to produce astaxanthin, this method adopts one-step method production process, improve the shortcomings such as the production cycle length that two-step approach production brings, complex process, greatly improved output and the quality of astaxanthin.Patent CN1966660A has invented a kind of large scale culturing Haematocoocus Pluvialls and has transformed the devices and methods therefor of astaxanthin, has solved Haematocoocus Pluvialls and has been limited to the shortcoming that short run is tested, and has greatly advanced the process of commercially producing.Patent CN1392250A discloses a kind of method that photoreactor is regulated haematococcus pulvialis population density and chemical activators and accumulation.Yet, mainly concentrate on about the research of haematococcus pluvialis to produce astaxanthin and cultivate the production aspect, and about the research of the extraction of astaxanthin and processing technology aspect seldom.Wang Lingzhao has compared the effect (food research and development, the 7th phase in 2008) of microwave method, cryogrinding extraction process and alkaline extraction extraction Determination of Astaxanthin in Haematococcus Pluvialis.From its result of study, the step of these extracting method is more, although extract has anti-oxidant ability with removing hydroxy radical qiao preferably, the concentration of astaxanthin is lower.Guo Wenjing etc. have studied the superelevation platen press and extracted the technique of astaxanthin from Haematocoocus Pluvialls, and the Determination of Astaxanthin in Haematococcus Pluvialis rate of transform can reach 98%, but 300MPa ultra-high voltage is difficult to reach the condition (agricultural mechanical journal, 5 phases in 2008) of industrial applications.
The traditional extraction process of astaxanthin has solvent-extraction process, microwave extraction method, enzyme extraction method, ultrahigh-pressure extraction method etc., yet these methods exist the shortcomings such as dissolvent residual, contaminate environment, complex process are arranged in extract.
Summary of the invention
The objective of the invention is to utilize supercritical extraction technique, provide a kind of from Haematocoocus Pluvialls the method for supercritical extraction astaxanthin, realize by following scheme:
The Haematocoocus Pluvialls powder of learning from else's experience and pulverizing, in the extraction kettle in the supercritical extraction unit of packing into, the temperature of setting extraction kettle is 30-50 ℃, pressure is 20-30MPa, and the temperature and pressure of separation reactor I is respectively 30-40 ℃, 8-10MPa, the temperature of separation reactor I I is 30-40 ℃, CO
2The flow velocity of fluid is 20-40L/h, and extraction time is 2-4h, and extract is emitted from separating still aperiodically, collects and is stored in container.
In extract, the content analysis of astaxanthin adopts the measuring method of U.S. Cyanotech company.
Supercritical liquid extraction technique is a kind of new bio separation and purification technology of just rising late 1970s.It does not have the separated from solvent problem of traditional solvent, thereby makes the extraction process of natural product that revolutionary improvement occur.Astaxanthin extraction thing by the inventive method obtains for the thick material of garnet, has kept active substance preferably, can be used for health food development.
Meaning of the present invention:
(1) extracting method is simple, quick, easy to operate;
(2) in extraction process, extraction solvent used is CO
2, the production process environmental protection, the extract organic solvent-free is residual;
(3) temperature during extraction process is lower, can effectively avoid the active substance pyrolytic decomposition;
(4) as shown in the content analysis result of astaxanthin, novel process can improve output and the quality of astaxanthin.
Embodiment
Below in conjunction with example, the present invention is described in further detail.
Embodiment one
Take the Haematocoocus Pluvialls powder 100g through pulverizing, be placed in the extraction kettle of 1L supercritical extraction unit (HA220-50-06 type), the temperature of setting extraction kettle is 40 ℃, and pressure is 30MPa, extraction agent CO
2Flow be 20L/h, the temperature and pressure of separation reactor I is respectively 35 ℃ and 10MPa, the temperature of separation reactor I I is 40 ℃, CO
2The flow velocity of fluid is 20-40L/h, and the dynamic extraction time is 2.5h continuously, and extract is emitted from separating still aperiodically, collects and is stored in container, and extract is bolarious thick material.Weigh, be total to get extract 8.0g, percentage extraction is 8.0%.
Embodiment two
With reference to embodiment one, take Haematocoocus Pluvialls powder 100g, be placed in the 1L extraction kettle, set extraction parameters, the extraction kettle temperature is 50 ℃, pressure is 20MPa, CO
2Flow be 25L/h, the separation reactor I temperature is 35 ℃, pressure is 9MPa, separation reactor I I temperature is 40 ℃, continuous dynamic extraction 2.5h extracts to separate from separating still after finishing and obtaining bolarious thick material.Weigh, be total to get extract 6.8g, percentage extraction is 6.8%.
Embodiment three
With reference to embodiment one, take Haematocoocus Pluvialls powder 100g, be placed in the 1L extraction kettle, set extraction parameters, the extraction kettle temperature is 40 ℃, pressure is 25MPa, CO
2Flow be 20L/h, the separation reactor I temperature is 30-35 ℃, pressure is 8MPa, separation reactor I I temperature is 35-40 ℃, continuous dynamic extraction 3h extracts to separate from separating still after finishing and obtaining bolarious thick material.Weigh, be total to get extract 7.4g, percentage extraction is 7.4%.
Embodiment four
With reference to embodiment one, take Haematocoocus Pluvialls powder 100g, be placed in the 1L extraction kettle, adding simultaneously 100ml ethanol is cosolvent.Set extraction parameters, the extraction kettle temperature is 40-42 ℃, and pressure is 30MPa, CO
2Flow be 20L/h, the separation reactor I temperature is 30-35 ℃, pressure is 8-9MPa, separation reactor I I temperature is 35-40 ℃, continuous dynamic extraction 2h, garnet liquid is collected on not timing ground from separating still.Then extracting solution is removed cosolvent in rotatory evaporator, obtained bolarious thick material.Weigh, be total to get extract 7.5g, percentage extraction is 7.5%.
Embodiment five
With reference to embodiment one, take Haematocoocus Pluvialls powder 600g, be placed in the 5L extraction kettle, set extraction parameters, the extraction kettle temperature is 40-42 ℃, pressure is 30MPa, extraction agent CO
2Flow be 40L/h, the separation reactor I temperature is 30-35 ℃, pressure is 8-10MPa, separation reactor I I temperature is 35-40 ℃, continuous dynamic extraction 4h, bolarious thick extract is collected on leaching process not timing ground from separating still.Merging is weighed, and is total to get extract 42.6g, and percentage extraction is 7.1%.
Embodiment six
In extract, the content analysis of astaxanthin adopts the measuring method of U.S. Cyanotech company.Take 10mg algae powder extracts, put into 10m L centrifuge tube, add 3g quartz sand, add 5mLDMSO in centrifuge tube, 30min is placed in 45-50 ℃ of water-bath, during vibration 3 times.Then the centrifugal 5min of 3000rpm makes not tolerant precipitation, and supernatant liquor is changed in the 25mL volumetric flask.Add 5mL acetone again in centrifuge tube, vortex vibration 30s, the centrifugation precipitation changes supernatant liquor in the 25mL volumetric flask over to, and acetone extracting at least 3 times is until supernatant liquor substantially colourless (light absorption value is less than 0.05).
Be settled to 25mL with acetone, cover volumetric flask, slight vibration mixes, and draws 5-7mL and puts into centrifuge tube, and recentrifuge is to remove the particulate matter of bringing in preceding step.Measure maximum light absorption value under the 474nm wavelength, acetone is made blank.
The standard substance of astaxanthin (CAS No:472-61-7, molecular formula: C
40H
52O
4) provided by Sigma company.Accurately take 2.0mg astaxanthin pure sample product, with acetone solution and be settled to 50mL, accurately pipette out respectively 0.5,1.0,1.5,2.0,2.5mL, with acetone diluted and be settled to 50mL, survey light absorption value in the same wave strong point.
In extract, the content of astaxanthin sees Table 1.
Table 1
| Embodiment | One | Two | Three | Four | Five |
| Extract yield, % | 8.0 | 6.8 | 7.4 | 7.5 | 7.1 |
| Content astaxanthin, % | 17.5 | 18.3 | 19.4 | 18.8 | 18.0 |
| The astaxanthin yield, % | 1.40 | 1.24 | 1.46 | 1.41 | 1.28 |
Claims (1)
1. the method for a supercritical extraction astaxanthin from Haematocoocus Pluvialls, realize by following scheme:
Take the Haematocoocus Pluvialls powder 100g through pulverizing, be placed in the extraction kettle of 1L supercritical extraction unit HA220-50-06 type, the temperature of setting extraction kettle is 40 ℃, and pressure is 30MPa, extraction agent CO
2Flow be 20L/h, the temperature and pressure of separating still I is respectively 35 ℃ and 10MPa, the temperature of separating still II is 40 ℃, CO
2The flow velocity of fluid is 20-40L/h, and the dynamic extraction time is 2.5h continuously, and extract is emitted from separating still aperiodically, collects and is stored in container, and extract is bolarious thick material, weighs, and is total to get extract 8.0g, and percentage extraction is 8.0%.
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| CN2009101022914A CN101691348B (en) | 2009-09-07 | 2009-09-07 | Method for extracting astaxanthin from haematococcus pluvialis |
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| CN2009101022914A CN101691348B (en) | 2009-09-07 | 2009-09-07 | Method for extracting astaxanthin from haematococcus pluvialis |
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Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102012363A (en) * | 2010-11-16 | 2011-04-13 | 云南绿A生物工程有限公司 | Method for measuring astaxanthin content in haematococcus pluvialis powder and extract |
| CN101991612B (en) * | 2010-11-16 | 2013-06-05 | 云南绿A生物工程有限公司 | Preparation method of haematococcus pluvialis extracts |
| CN102911095A (en) * | 2011-10-26 | 2013-02-06 | 江苏江大源生态生物科技有限公司 | Method for supercritical CO2 extraction of effective ingredients of Haematococcus pluvialis spore powder |
| CN102525837B (en) * | 2011-11-08 | 2013-09-11 | 海南光宇生物科技有限公司 | Astaxanthin-containing bacterial cellulose membrane product |
| CN103044304B (en) * | 2012-12-21 | 2014-12-03 | 宁波红龙生物科技有限公司 | Method for preparing astaxanthin extractive from haematococcus pluvialis powder |
| CN104326958A (en) * | 2013-12-14 | 2015-02-04 | 沈阳药科大学 | Method for extracting astaxanthin from haematococcus pluvialis |
| CN103880726B (en) * | 2014-03-14 | 2016-03-02 | 山东省农业科学院农产品研究所 | Enzyme process and polishing work in coordination with the method for organic solvent extraction Determination of Astaxanthin in Haematococcus Pluvialis |
| CN104502289A (en) * | 2014-12-18 | 2015-04-08 | 贵州航天乌江机电设备有限责任公司 | Measurement method for detecting astaxanthin content |
| CN104910057A (en) * | 2014-12-18 | 2015-09-16 | 贵州航天乌江机电设备有限责任公司 | Refining method for Haematoccus pluvialis astaxanthin |
| CN104945297A (en) * | 2015-05-18 | 2015-09-30 | 万庆家 | Method for extracting astaxanthin from haematococcus pluvialis |
| CN105503684B (en) * | 2016-01-27 | 2018-02-02 | 华南理工大学 | A kind of supercritical CO2The method for extracting Astaxanthin In Haematococcus Pluvialis |
| CN106748944A (en) * | 2016-12-01 | 2017-05-31 | 南京工业大学 | Method for extracting astaxanthin by haematococcus pluvialis cell rapid wall-breaking coupling |
| CN109481333A (en) * | 2018-12-13 | 2019-03-19 | 昆明白鸥微藻技术有限公司 | A kind of astaxanthin EGF stoste and preparation method thereof |
| CN110357799A (en) * | 2019-07-29 | 2019-10-22 | 云南钰腾生物科技有限公司 | A kind of extraction element and extraction process of astaxanthin |
| CN111214846B (en) * | 2019-11-29 | 2022-05-17 | 云南绿A生物产业园有限公司 | Haematococcus pluvialis extract and preparation method thereof |
| CN112979522A (en) * | 2021-03-15 | 2021-06-18 | 南京中源盛生物科技研究院有限公司 | Method for extracting astaxanthin from haematococcus pluvialis |
| CN113292469B (en) * | 2021-06-28 | 2022-11-25 | 浙江皇冠科技有限公司 | Method for extracting phaffia rhodozyma intracellular astaxanthin by using beta-glucanase and chitinase |
| WO2023236111A1 (en) * | 2022-06-08 | 2023-12-14 | 台湾中油股份有限公司 | Method for extracting sarcodia montagneana, sarcodia montagneana extract, and use thereof |
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