CN101683362A - Method for extracting ganoderma oil by supercritical carbon dioxide - Google Patents
Method for extracting ganoderma oil by supercritical carbon dioxide Download PDFInfo
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- CN101683362A CN101683362A CN200910180842A CN200910180842A CN101683362A CN 101683362 A CN101683362 A CN 101683362A CN 200910180842 A CN200910180842 A CN 200910180842A CN 200910180842 A CN200910180842 A CN 200910180842A CN 101683362 A CN101683362 A CN 101683362A
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- ganoderma
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- oil
- supercritical carbon
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- 241000222336 Ganoderma Species 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 40
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 229910002092 carbon dioxide Inorganic materials 0.000 title claims abstract description 17
- 239000001569 carbon dioxide Substances 0.000 title claims abstract description 17
- 238000003815 supercritical carbon dioxide extraction Methods 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims description 14
- 239000002994 raw material Substances 0.000 claims description 12
- 238000012856 packing Methods 0.000 claims description 7
- 210000000582 semen Anatomy 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 5
- 238000000465 moulding Methods 0.000 claims description 3
- 238000011282 treatment Methods 0.000 claims description 3
- 239000011343 solid material Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 22
- 239000002777 nucleoside Substances 0.000 abstract description 13
- 150000003833 nucleoside derivatives Chemical class 0.000 abstract description 13
- 238000004519 manufacturing process Methods 0.000 abstract description 9
- 150000003648 triterpenes Chemical class 0.000 abstract description 9
- 229930182558 Sterol Natural products 0.000 abstract description 8
- 229930013930 alkaloid Natural products 0.000 abstract description 8
- 150000003797 alkaloid derivatives Chemical class 0.000 abstract description 8
- 150000003432 sterols Chemical class 0.000 abstract description 8
- 235000003702 sterols Nutrition 0.000 abstract description 8
- 238000012545 processing Methods 0.000 abstract description 6
- 239000002904 solvent Substances 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 241000233866 Fungi Species 0.000 abstract description 3
- 240000008397 Ganoderma lucidum Species 0.000 abstract description 3
- 235000001637 Ganoderma lucidum Nutrition 0.000 abstract description 3
- 150000001413 amino acids Chemical class 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 150000004676 glycans Chemical class 0.000 abstract description 2
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 2
- 229920001282 polysaccharide Polymers 0.000 abstract description 2
- 239000005017 polysaccharide Substances 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract 2
- 150000002500 ions Chemical class 0.000 abstract 1
- 239000000203 mixture Substances 0.000 description 26
- 239000003921 oil Substances 0.000 description 17
- 239000007788 liquid Substances 0.000 description 12
- 239000000470 constituent Substances 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 239000004615 ingredient Substances 0.000 description 5
- 239000007901 soft capsule Substances 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- 241000050051 Chelone glabra Species 0.000 description 1
- 241000283986 Lepus Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000007230 Sorghum bicolor Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000199 molecular distillation Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for extracting ganoderma oil by supercritical carbon dioxide, belonging to the field of deep processing of edible and medicinal fungi, in particular to a processing technology of ganoderma. The technology of the invention has the following advantages: adopts a modern technology to extract natural active ingredients of the lucid ganoderma, and the supercritical carbon dioxide extraction technology has a low-temperature inert environment, does not influence the activity of the active ingredients, is nonflammable, safe to use, does not pollute the environment, and has no solvent residue and no heavy metal ions. Therefore, the new technology for processing the ganoderma lucidum by adopting the supercritical technology has practical value. Can be used to obtain Ganoderma oil, Ganoderma triterpene component, Ganoderma nucleoside component, Ganoderma sterol, and alkaloid, and can be combined with other methods to obtain many products such as protein, amino acids, and Ganoderma polysaccharide. The method for producing the effective components of the ganoderma lucidum by adopting the supercritical technology has great economic value and social value.
Description
The application is an application number 200610000663.9 on January 11 2006 applying date
Denomination of invention: the dividing an application of a kind of method of producing effective component of glossy ganoderma by using supercritical technology.
Technical field
The invention belongs to the process technology in the deep processing field, particularly Ganoderma of edible medicinal fungi.
Background technology
Production Ganoderma in all parts of the country was 60478 tons in 2004, and through years of development, Ganoderma has been developed into the situation of multiple converted products from unidirectional outlet, and the output value of series of products is above 3,000,000,000 yuan.
Look back the history of artificial culture Ganoderma, through document relatively: CN1247019 introduces the manufacture method of kernel culture.CN1075594 relates to the manufacture method of strain.CN206043 and CN 1317564 utilize rice, Semen setariae, Sorghum vulgare Pers. to be culture medium, make mycelial growth and are enclosed in around the corn generation rice-koji.CN297773, CN1233389b are different with technical purpose of the present invention, concrete grammar and meaning.CN1099217 is mycelial technological invention of producing in 96 hours.CN1484981 is the Ganoderma converted products.Through searching document, do not have and the eclipsed method of the present invention.The raw material that does not utilize CN1706238A to produce carries out deep processing, does not implement the method for producing effective component of glossy ganoderma by using supercritical technology.
Summary of the invention
For overcoming above-mentioned defective, patent purpose of the present invention is: overcome the shortcoming of traditional extraction technology, a kind of method that adopts extracting ganoderma oil by using supercritical carbon dioxide is provided, make it to become new product, new raw material in nourishing healthy and the field of medicaments.
For achieving the above object, the method of a kind of extracting ganoderma oil by using supercritical carbon dioxide of the present invention, it is characterized in that: the finished product of cultivating Ganderma lucidum with Semen Tritici aestivi is a raw material, adopts supercritical carbon dioxide extraction method to acquire Ganoderma oil, through preliminary treatment, molding, packing method.
Process route is:
Raw material with the Wheat Production Ganoderma----pulverize----supercritical carbon dioxide extraction----Ganoderma oil does not need entrainer----other compositions use different concentration ethanol to be entrainer----entering separating still presses----obtain effective ingredient----be condensed into paste or Powdered----under the GMP condition, pass through preliminary treatment, molding, packing method.
In the said method, the finished product of cultivating Ganderma lucidum with Semen Tritici aestivi is a raw material, and these raw materials are sporophore (comprising button), mycelium and Ganoderma spore powders of Ganoderma.The finished product of cultivating Ganderma lucidum with Semen Tritici aestivi is a raw material, is meant the raw material of the method production that utilizes CN1706238A, obtains sporophore (comprising button), mycelium and the Ganoderma spore powder of Ganoderma, is a kind of new method, good product quality, effective ingredient height.Particularly the mycelium of Semen Tritici aestivi cultivation partly has very big exploitation to be worth, and is one of important source material of the present invention.Pretreated feedstock should be selected cleaning, does not have impurity, exsiccant finished product.
In the said method, solid material is pulverized the back and used, and granularity is the 20-60 order, and is best about 40 orders.Through pulverizing the effect that can improve carbon dioxide abstraction.
In the said method, extraction kettle pressure 15-50Mpa be separating still pressure be 5-30Mpa promptly in same flow process, the former pressure is greater than latter's pressure.The pressure of extraction kettle is at 15-50Mpa, and adopting the flow of carbon dioxide is 15-25 liter/h, and according to being extracted into branch design temperature, the scope of application of extraction kettle is at 30-80 ℃, usually at 50-60 ℃.Effective ingredient promptly has than higher dissolubility during our said supercriticality under the pressure and temperature of needs, and Ganoderma oil, Ganoderma triterpenoids constituents, Ganoderma nucleoside composition, Ganoderma sterol and alkaloid all have these characteristics.Can obtain Ganoderma oil under certain condition.Changing pressure and temperature can make ganoderma lucidum triterpene composition and Ganoderma nucleoside composition have than higher dissolubility.Can not obtain very purified single component through first separation, be the mixture based on a composition.Change through super critical condition several times, obtaining Ganoderma oil respectively is main mixture, or the Ganoderma triterpenoids constituents is main mixture, or Ganoderma nucleoside composition is main mixture, or Ganoderma sterol and alkaloid are main mixture.In conjunction with other method, ion exchange, molecular distillation can further improve the purity of single component.Need whole part just can merge above the composition that obtains of super critical condition several times, through being processed into Ganoderma oral liquid, Ganoderma tablet, Ganoderma tea, LINGZHI JIAONANG, soft capsule of glossy ganoderma, Ganoderma spore fat capsule, Ganoderma oil soft capsule, Ganoderma granule.
In the said method, supercritical technology uses carbon dioxide to carry out the composition extraction as solvent.Become liquid because carbon dioxide is a solvent under condition of high voltage, effective ingredient can be dissolved in the carbon dioxide, reduces in the separating still internal pressure, and carbon dioxide becomes gas and reclaims, perhaps volatilization.So the CO 2 supercritical technology does not influence the effective ingredient activity, and is nonflammable, safe in utilization, do not pollute the environment no solvent residue.
In the said method, that utilizes supercritical extraction method at first obtains the material of Ganoderma oil for main component.Because Ganoderma oils just can obtain under condition relatively easily, so, at first carry out the production of effective component of glossy ganoderma.
The technology of the present invention has following advantage: adopt state-of-the-art technology to extract the Ganoderma active skull cap components, supercritical carbon dioxide extraction method possesses the low temperature inert environments, 1, do not influence the effective ingredient activity, 2, nonflammable, 3, safe in utilization, 4, do not pollute the environment, 5, no solvent residue, the heavy metal free ion.Therefore, adopt supercritical technology to create the new technology of Ganoderma processing, with practical value.Can obtain Ganoderma oil, Ganoderma triterpenoids constituents, Ganoderma nucleoside composition, Ganoderma sterol, alkaloid, can obtain many products of protein, aminoacid, ganoderan, develop the wide purposes of Ganoderma in conjunction with other method ion exchange, the sedimentation method.So the technology of the present invention is produced effective component of glossy ganoderma huge economic and social value.
Illustrate below by example: example one
4 kilograms of the sporophore of Ganoderma (comprising original hase), 8 kilograms of Ganoderma myceliums are pulverized the back and are used, and granularity is about 40 orders.Method is: extraction kettle pressure 25Mpa is that separating still pressure is 15Mpa, and adopting the flow of carbon dioxide is 15-25 liter/h, and the scope of application of extraction kettle is at 50 ℃.Collect 0.6 kilogram of liquid in 30 minutes later on, through centrifugal, obtain 0.2 kilogram of the Ganoderma oil on upper strata.Lower floor's liquid is Ganoderma triterpenoids constituents, Ganoderma nucleoside composition, Ganoderma sterol and alkaloidal mixture.Continue to use supercritical technology, extraction kettle pressure 30Mpa is that separating still pressure is 10Mpa, and adopting the flow of carbon dioxide is 20 liters/h, and the temperature of extraction kettle is at 45 ℃.Working concentration is an entrainer at 20% ethanol, flow 500ml/h.Collect 0.6 kilogram of liquid, what obtain is that Ganoderma nucleoside composition and alkaloid are main liquid.
Ganoderma oil is through dewatering, and after the filtration, packing becomes commodity.Ganoderma oil can be processed into cosmetics, health food, soft capsule.Lower floor's Ganoderma triterpenoids constituents, Ganoderma nucleoside composition, Ganoderma sterol and alkaloidal mixture and Ganoderma nucleoside composition and alkaloid are that main liquid merges, and the method for process vacuum drying or other physical drynesses becomes solid, and weight is 50 gram powder.Packing becomes commodity, can be processed into tablet, the raw material of soft capsule.
Example two,
18 kilograms of Ganoderma myceliums are pulverized the back and are used, and granularity is about 40 orders.Method is: extraction kettle pressure 20Mpa is that separating still pressure is 12Mpa, and adopting the flow of carbon dioxide is 20 liters/h, and the scope of application of extraction kettle is at 50 ℃.Collect 0.92 kilogram of liquid in 30 minutes later on, through centrifugal, obtain 0.28 kilogram of the Ganoderma oil on upper strata.Lower floor's liquid is Ganoderma triterpenoids constituents, Ganoderma nucleoside composition, Ganoderma sterol and alkaloidal mixture.Continue to use supercritical technology, extraction kettle pressure 30Mpa is that separating still pressure is 15Mpa, and adopting the flow of carbon dioxide is 20 liters/h, and the temperature of extraction kettle is at 45 ℃.Working concentration is an entrainer at 10% ethanol, flow 500ml/h.Collect 0.7 kilogram of liquid, what obtain is that Ganoderma nucleoside composition and alkaloid are main liquid.
Ganoderma oil is through dewatering, and after the filtration, packing becomes commodity.Lower floor's Ganoderma triterpenoids constituents, Ganoderma nucleoside composition, Ganoderma sterol and alkaloidal mixture and Ganoderma nucleoside composition and alkaloid are that main liquid merges, method through vacuum drying or other physical drynesses becomes solid, weight is 68 gram powder, and packing can become commodity.
Handle through supercritical technology, extracting later mycelium has 16.5 kilograms, wherein also contains a large amount of starch, polysaccharide, aminoacid, protein and vitamin.Also have edible exploitation to be worth in conjunction with the technology of having known.
List of references: 1, Lin Zhibin: the modern study of Ganoderma, publishing house of Beijing Medical University, March calendar year 2001 the 2nd edition 94,157,310
2, Lin Shuqian: Chinese medicinal fungus production and exploitation, Chinese agriculture publishing house, November in 2000 the 1st edition 211,257.
Claims (4)
1, a kind of method of extracting ganoderma oil by using supercritical carbon dioxide is characterized in that: the finished product of cultivating Ganderma lucidum with Semen Tritici aestivi is a raw material, adopts supercritical carbon dioxide extraction method to acquire Ganoderma oil, through the method for preliminary treatment, molding, packing.
2, method according to claim 1 is characterized in that: the finished product of cultivating Ganderma lucidum with Semen Tritici aestivi is a raw material, and these raw materials are sporophore (comprising button), mycelium and Ganoderma spore powders of Ganoderma.
3, method according to claim 1 is characterized in that: solid material is pulverized the back and used, and granularity is the 20-60 order, and is best about 40 orders.
4, method according to claim 1 is characterized in that: extraction kettle pressure 15-50Mpa be separating still pressure be 5-30Mpa promptly in same flow process, the former pressure is greater than latter's pressure.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910180842A CN101683362A (en) | 2006-01-11 | 2006-01-11 | Method for extracting ganoderma oil by supercritical carbon dioxide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910180842A CN101683362A (en) | 2006-01-11 | 2006-01-11 | Method for extracting ganoderma oil by supercritical carbon dioxide |
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| CN2006100006639A Division CN100998613B (en) | 2006-01-11 | 2006-01-11 | Method for producing effective component of glossy ganoderma by using supercritical technology |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101845361A (en) * | 2010-06-18 | 2010-09-29 | 福建仙芝楼生物科技有限公司 | Method of extraction and grading purification of ganoderma lucidum spore oil by supercritical CO2 |
| CN102524752A (en) * | 2011-01-04 | 2012-07-04 | 北京电子科技职业学院 | Method for extracting volatile components from coprinus comatus |
| CN103652836A (en) * | 2013-11-28 | 2014-03-26 | 仙芝科技(福建)股份有限公司 | Lucid ganoderma decoction piece processing method with capacity of increasing ganoderan dissolution rate |
| CN105232584A (en) * | 2015-09-30 | 2016-01-13 | 河南行知专利服务有限公司 | Decoction-free extraction process for precious Chinese herbal medicines |
| CN111662393A (en) * | 2020-07-01 | 2020-09-15 | 广东悦生生物科技有限公司 | Method for extracting total components of lucid ganoderma |
| CN114631598A (en) * | 2022-04-09 | 2022-06-17 | 吉林省爱康寿生物科技有限公司 | Non-waste preparation treatment process for industrialization of cut-log ganoderma lucidum |
-
2006
- 2006-01-11 CN CN200910180842A patent/CN101683362A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101845361A (en) * | 2010-06-18 | 2010-09-29 | 福建仙芝楼生物科技有限公司 | Method of extraction and grading purification of ganoderma lucidum spore oil by supercritical CO2 |
| CN101845361B (en) * | 2010-06-18 | 2012-07-04 | 福建仙芝楼生物科技有限公司 | Method of extraction and grading purification of ganoderma lucidum spore oil by supercritical CO2 |
| CN102524752A (en) * | 2011-01-04 | 2012-07-04 | 北京电子科技职业学院 | Method for extracting volatile components from coprinus comatus |
| CN103652836A (en) * | 2013-11-28 | 2014-03-26 | 仙芝科技(福建)股份有限公司 | Lucid ganoderma decoction piece processing method with capacity of increasing ganoderan dissolution rate |
| CN103652836B (en) * | 2013-11-28 | 2015-07-15 | 仙芝科技(福建)股份有限公司 | Lucid ganoderma decoction piece processing method with capacity of increasing ganoderan dissolution rate |
| CN105232584A (en) * | 2015-09-30 | 2016-01-13 | 河南行知专利服务有限公司 | Decoction-free extraction process for precious Chinese herbal medicines |
| CN111662393A (en) * | 2020-07-01 | 2020-09-15 | 广东悦生生物科技有限公司 | Method for extracting total components of lucid ganoderma |
| CN114631598A (en) * | 2022-04-09 | 2022-06-17 | 吉林省爱康寿生物科技有限公司 | Non-waste preparation treatment process for industrialization of cut-log ganoderma lucidum |
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Open date: 20100331 |