CN101668859A - Has plant of enhanced yield correlated character and preparation method thereof - Google Patents

Abstract

Relate generally to biology field of the present invention also relates to the method that is used for strengthening important yield correlated character on the plant diversified economy.More specifically, the present invention relates in plant, strengthen the method for output correlated character by the expression of nucleic acids of regulating coding Harpin binding factor G polypeptide (hereinafter being called " HpaG ") in the plant.The invention still further relates to the plant of being regulated expression of the nucleic acid with coding HpaG polypeptide, described plant has the enhanced yield correlated character with respect to control plant.The present invention also provides the construct that comprises the HpaG coding nucleic acid, and it can be used for implementing method of the present invention.The present invention also provides the method that strengthens the output correlated character by the expression of nucleic acids of regulating coding SWITCH 2/SUCROSENON-FERMENTING 2 (SWI2/SNF2) polypeptide in (preferred increasing) plant in plant with respect to control plant.The invention still further relates to the plant of being regulated expression of the nucleotide sequence with coding SWI2/SNF2 polypeptide, described plant has the enhanced yield correlated character with respect to control plant.The present invention also is provided for implementing the construct of the inventive method.

Description

Has plant of enhanced yield correlated character and preparation method thereof

Relate generally to biology field of the present invention also relates to the method that is used for strengthening important yield correlated character on the plant diversified economy.More specifically, the present invention relates in plant, strengthen the method for output correlated character by the expression of nucleic acids of regulating coding Harpin binding factor G polypeptide (hereinafter being called " HpaG ") in the plant.The invention still further relates to the plant of being regulated expression of the nucleic acid with coding HpaG polypeptide, described plant has the enhanced yield correlated character with respect to control plant.The present invention also provides the construct that comprises the HpaG coding nucleic acid, and it can be used for implementing method of the present invention.The present invention also provides by regulating in (preferred increasing) plant and has encoded SWITCH 2/ SUCROSE NON- FERMENTING 2(SWI2/SNF2) expression of nucleic acids of polypeptide strengthens the method for output correlated character with respect to control plant in plant.The invention still further relates to the plant of being regulated expression of the nucleotide sequence with coding SWI2/SNF2 polypeptide, described plant has the enhanced yield correlated character with respect to control plant.The present invention also is provided for implementing the construct of the inventive method.

The world population of sustainable growth is supplied the research that atrophy has stimulated relevant increase farm efficiency with agricultural with the arable land.The plant that conventional crop and the utilization of Horticulture improved means select breeding technique to have welcome characteristic with evaluation.Yet this type of selects breeding technique to have several defectives, and promptly these technology generally expend a lot of work and produce the plant that often contains the heterology hereditary component, and described heterology hereditary component may always not cause desired proterties to be transmitted from the parental generation plant.Recent advances in molecular biology has allowed the germplasm of human improvement animal and plant.The genetic engineering of plant makes and can separate and operate genetic material (generally being in DNA or rna form) and subsequently with in this genetic material importing plant.This type of technology has generation and possesses diversified economy, agronomy or the crop of Horticulture improvement proterties or the ability of plant.

Proterties with special economic meaning is the output that increases.But output is normally defined the measuring result from the economic worth of crop.This result can define with regard to quantity and/or quality aspect.Output directly depends on Several Factors, for example the number of organ and size, plant structure (for example number of branch), seed generation, leaf aging etc.The important factor that root development, nutrient intake, stress tolerance and early stage vigor (early vigor) also can be decision output.Optimize aforementioned factor thereby can contribution be arranged increasing crop yield.

Seed production is the proterties that is even more important, because the seed of many plants is extremely important for human and animal's nutrition.Crop such as cereal, rice, wheat, canola oil dish (canola) and soybean account for the over half of human total calorie of intake, no matter be direct consumption by seed itself, and the still consumption by the meat products of raising by the seed of processing.They also are the sources of the used carbohydrate of industrial processes, oils and multiclass metabolite.Seed contains embryo (the new bud and the source of root) and endosperm (nutrition source of embryonic development in sprouting and the seedling early growth process).The growth of seed relates to many genes, and needs metabolite to be transferred to the seed of growing from root, leaf and stem.Particularly endosperm absorbs carbohydrate, oils and proteinic metabolic precursor thereof, it is synthesized the storage polymer, so that the grains are plump.

Harvest index is a seed production and the ratio of over-ground part dry weight, under many envrionment conditionss, be metastable, therefore can obtain the sane dependency (for example, Rebetzke et al. (2002) Crop Science 42:739) of plant size and grain output usually.These methods are relative internal associations, because the Photosynthetic Productivity (Gardener et al. (1985) Physiology of Crop Plants.Iowa StateUniversity Press, pp 68-73) that grain biomass major part depends on the current of the leaf of plant and stem or stores.Therefore, select (even etap) in early days to be used as index (for example, Tittonell et al. (2005) AgricEcosys﹠amp of potential production in the future to the plant size; Environ 105:213).When test hereditary difference during to the influencing of stress tolerance, the ability of stdn soil property, temperature, water and nutrition operability and light intensity is the inherent advantage that greenhouse or plant-growth room environmental are compared with the field.Yet, because the pollination that do not exist wind or insect to cause weakens or the insufficient space of matured root or canopy growth etc. can limit the use of these controlled environments in the test volume variance to the artificial restriction of output.Therefore, the size of measuring plant in the early development under the normalization condition in growth room or greenhouse provides the standard practices of potential hereditary output advantage indication.

Especially another proterties that has importance economically is the enhanced yield correlated character of growing plants under the abiotic stress condition.Abiotic stress is the major cause of crop failure in the world, reduces the mean yields of most staple crop plants more than 50% (Wang et al., Planta (2003) 218:1-14).Abiotic stress can be caused by arid, salinity, temperature limitation, chemical toxicity and oxidative stress.The ability that strengthens the output correlated character under the abiotic stress condition in the growing plants has the great economy advantage in the world peasant and will allow at unfavourable condition and crop-planting otherwise with impossible area kind plant.

The ability that increases plant biomass will have many application in such as fields such as agriculturals, comprise production, arboriculture, gardening and the forest industry of ornamental plant.The algae that the output that increases also can be used for using at bio-reactor produces (be used for the biotechnology production such as materials such as medicine, antibody or vaccines, or be used for the bio-transformation of organic waste) and other these class fields.

Background

I.HARPIN

III type excretory system (TTSS) is the special output device of gram negative bacterium and is found in the plant and animal pathogenic agent, and in the endosymbiosis root nodule bacterium (Rhizobia).Infer TTSS with protein delivery in bacterium bonded host cell.In plant pathogenetic bacteria, TTSS is that one group of hypersensitivity is replied and Disease-causing gene, and it comprises about 20 kinds of gene: Hrp bunches.In these genes 9 kinds (Harpin conservative or hrc) guard in the plant and animal pathogenic agent, eight kinds of genes with the coding flagellum apparatus in them have homology (Bogdanove et al., Mol.Microbiol.20,681-683,1996), the 9th kind of hrcC and GSP adventitia secretin homology (Deng and Huang, J.Bacteriol.180,4523-4531,1999).Hpa (hrp-is correlated with) gene promotes pathogenic and induced hypersensitivity reaction (HR) in the nonhost plant, but interacts optional for the cause of disease of bacterium and plant.Infer that flagellum apparatus and TTSS are from common origin evolution (Gophna et al., Gene312,151-163,2003); TTSS is by the further diffusion (Nguyen et al., J.Mol.Microbiol.Biotechnol.2,125-144,2000) in the remote bacterial species on evolving of a plurality of horizontal transfer incidents.

Many Gram-negative plant malignant bacterias have two groups of genes, and it regulates the interaction of they and plant.Non-toxic gene has determined host specificity based on gene-gene interaction, and hrp (anaphylaxis and pathogenic) gene relates to pathogenic and the inducing of anaphylaxis (HR) in the nonhost plant.HR is highly circumscribed vegetable cell death, when the resistance cultivar of nonhost plant or host plant by phytopathogen or HR trigger molecule, HR takes place when infiltrating as Avr albumen and Harpin.Think that HR is the resistance reaction of plant to microbial pathogen.

Harpin is one group of HR trigger molecule, and it is by III type Secretory Pathway (TTSS) secretion and cause HR when penetrating into the apoplast of nonhost plant leaf.Unlike Avr albumen (it must be delivered in the cell to bring into play their function), Harpin can cause HR when being delivered to the iuntercellular space of vegetable cell.Since first kind of Harpin HrpN since separate starch Erwinia (Erwiniaamylovora) and identify, reported the many Harpin from many species, described species comprise Rhodopseudomonas (Pseudomonas), the logical Bordetella (Ralstonia) in Rolls and xanthomonas (Xanthomonas).Harpin is the heat stable protein that is rich in glycine, and it lacks halfcystine, infers to be present in all the plant malignant bacterias with TTSS (Alfano and Colmer, Annu.Rev.Phytopathol.42,385-414,2004).The chemism of Harpin initiation HR is still unclear in the nonhost plant.The cause a disease HrpZ of mutation (Pseudomonas syringae pv.Syringae) of pseudomonas syringae cloves combines with the cell walls rather than the cytolemma of vegetable cell, and this protein is not from the protoplastis that lacks cell walls (the Hoyoset al.Mol.Plant-Microbe Interact.9 that induces reaction, 608-616,1996).Yet the cause a disease HrpZ of mutation (P.syringae pv.Phaseolicola) of pseudomonas syringae Kidney bean is double-deck and form ionic conduction hole (Lee et al., Proc.Natl.Acad.Sci.USA 98,289-294,2001) in conjunction with lipid.Terminal 109 amino acid of the N-of HrpZ can cause the level of HR similar to total length HrpZ (Alfano et al., Mol.Microbiol.19,715-728,1996) with terminal 216 amino acid of C-.People such as people such as Kim and Charkowski show that separating the cause a disease HrpW Harpin of mutation (P.syringae pv.Tomato) of starch Erwinia (E.amylovora) and pseudomonas syringae tomato is made up of terminal Harpin structural domain of two structural domains-N-and terminal Pel (pectate lyase) structural domain of C-, and propose HrpW and act on cell walls (Charkowski et al., J.Bacteriol.180,5211-5217,1998; Kim and Beer, J.Bacteriol.180,5203-5210,1998).

Except Harpin, the TTSS in the bacterium bunch of gene that also comprises coding Harpin binding factor.The HpaG polypeptide is less than Harpin, and they have very little sequence homology.Infer that these and the difference of Harpin cause different (Kim et al., J.Bacteriol.186,6239-6247,2004) that cause the HR ability between HpaG polypeptide and the Harpin in plant.

Korean patent application KR20030068302 discloses xanthomonas HpaG albumen, and it gives disease resistance when being applied to plant or plant seed, especially the resistance that carpet straw colour Zymomonas mobilis (carpetweed Xanthomonas campestris) is infected.The Harpin correlation factor has been used to give resistance in plant; And because the result of this biology stress resistance, plant is compared with control plant under biological stress conditions has higher output.

Be surprisingly found out that now the expression of nucleic acids of regulating coding Harpin correlation factor G polypeptide (HpaG) in the plant produces the plant that has the enhanced yield correlated character with respect to control plant.In the plant that does not have exposure and coerce, these enhanced yield correlated character have been obtained.

II.SNF2

The present invention relates to by in plant, increasing coding SWITCH 2/ SUCROSE NON- FERMENTING 2The expression of the nucleotide sequence of polypeptide (SWI2/SNF2) strengthens the method for output correlated character with respect to control plant in plant.

The cell processes that many karyomit(e)s are relevant, as duplicate, transcribe, DNA repairs or reorganization, needs come-at-able DNA.In order to handle these incidents, cell has such activity, and it can use the ATP hydrolysis to reinvent chromatin in eukaryote or destroy other DNA in protokaryon and eukaryote: protein complex.A most detailed example of these active researchs is that the ATP enzyme by SWI2/SNF2 family carries out, and described family relates to a big histone matter of many different sample processes of reinventing.

The SWI2/SNF2 family protein is ubiquitous, because they all are found in bacterium, archeobacteria and eukaryote.To comprise 7 conservative sequence motifs (I, Ia, II, III, IV, V and VI) SWI2/SNF2 ATP enzymatic structure territory carry out the multisequencing comparison after, they have been categorized as 24 different subfamilies (Flaus et al. (2006) Nucleic Acids Res.2006 recently; 34 (10): 2887-2905).These subfamilies adopt prototype member's title usually.A subfamily is named as SSO1653, (Flaus et al., supra after unique SWI2/SNF2 family member in archeobacteria sulfolobus solfataricus (Sulfolobus solfataricus); Duur et al. (2005) Cell121 (3): 363-373), SSO1653 is archeobacteria and the eubacterium subfamily that is similar to the proteic uniqueness of eukaryote SWI2/SNF2 most.The SSO1653 subfamily carries all SWI2/SNF2 family's family sequence and structure tags.

U.S. Patent application US2003/233670 has described the protein of polynucleotide and described polynucleotide encoding.SEQ ID NO:125 is the polynucleotide sequence of coding from the SWI2/SNF2 polypeptide of the SSO1653 subfamily of cytoalgae (Synechocystis sp.) PCC 6803.U.S. Patent application US2005/108791 has described 24149 nucleic acid and peptide sequence, wherein the SWI2/SNF2 polypeptide from the SSO1653 subfamily of cytoalgae PCC 6803 of the nucleic acid sequence encoding of SEQ ID NO:57 representative such as SEQ ID NO:396 representative.

Now be surprisingly found out that the expression of nucleotide sequence in plant that increases coding SWI2/SNF2 polypeptide produced the plant that has the enhanced yield correlated character with respect to control plant.

Definition

Polypeptides

Term " polypeptide " and " protein " are used interchangeably in this article and refer to be in amino acid in the random length polymerized form.

Polynucleotide/nucleic acid/nucleotide sequence/nucleotide sequence

Term " polynucleotide ", " nucleotide sequence ", " nucleotide sequence " are used interchangeably in this article and refer to be in Nucleotide in the random length polymerized form, i.e. ribonucleotide or deoxyribonucleotide or the combination of these two.

Control plant

To select suitable control plant be the habitual part that is provided with of experiment and can comprise the corresponding wild-type plant or not have the corresponding plant of goal gene.Control plant generally is plant species or or even the identical mutation identical with plant to be assessed.Control plant also can be the inefficacy zygote of plant to be assessed." control plant " not only refers to whole strain plant as used in this article, also refers to plant part, comprises seed and plants subdivision.

Homologue

Proteinic " homologue " comprises such peptide, oligopeptides, polypeptide, protein and enzyme, and they have aminoacid replacement, disappearance and/or insertion and have similar biologic activity and functionally active to the non-modifying protein of described peptide, oligopeptides, polypeptide, protein and enzyme source with respect to the above-mentioned protein of non-modification.

Disappearance refers to remove one or more amino acid from protein.

Insertion refers to the introducing in the predetermined site in protein of one or more amino-acid residues.Insertion can comprise in the fusion of single or multiple amino acid whose N end and/or fusion of C end and the sequence inserts.Usually, merge than the N end or the little about 1-10 of a C end fusion residue rank in the insertion meeting of aminoacid sequence inside.The example of N end or C end fusion rotein or fusogenic peptide comprise as the binding domains of used transcriptional activator in the yeast two-hybrid system or activation structure territory, bacteriophage coat protein, (Histidine)-6-label, glutathione S-transferase-label, A albumen, maltose binding protein, Tetrahydrofolate dehydrogenase, Tag100 epi-position, c-myc epi-position,

-epi-position, lacZ, CMP (calmodulin binding peptide), HA epi-position, C albumen epi-position and VSV epi-position.

Replace other amino acid that refer to have similar characteristics (as the tendency of similar hydrophobicity, wetting ability, antigenicity, formation or destruction α-Luo Xuanjiegou or β-laminated structure) and replace proteinic amino acid.Aminoacid replacement generally is single residue, but can be a bunch collection property, and this depends on the functional constraint that places polypeptide; Inserting can be about 1-10 amino-acid residue rank usually.Aminoacid replacement preferably conservative amino acid replaces.Conservative property replacement table is (seeing for example Creighton (1984) Proteins.W.H.Freeman and Company and following table 1) well-known in the art.

Table 1: the example that conservative amino acid replaces

Residue	Conservative property replaces	Residue	Conservative property replaces
				????Ala	????Ser	??Leu	????Ile；Val
????Arg	????Lys	??Lys	????Arg；Gln
				????Asn	????Gln；His	??Met	????Leu；Ile
????Asp	????Glu	??Phe	????Met；Leu；Tyr
				????Gln	????Asn	??Ser	????Thr；Gly
????Cys	????Ser	??Thr	????Ser；Val
				????Glu	????Asp	??Trp	????Tyr
????Gly	????Pro	??Tyr	????Trp；Phe
				????His	????Asn；Gln	??Val	????Ile；Leu
????Ile	????Leu，Val

Aminoacid replacement, disappearance and/or insert and to use the peptide synthetic technology well-known in the art such as the solid phase method of peptide synthesis etc. or by the recombinant DNA operation and carry out easily.Being used to operate dna sequence dna is well-known in the art with the method that produces proteinic replacement, insertion or disappearance variant.For example, it is well-known and comprise M13 mutagenesis, T7-Gen vitro mutagenesis method (USB to be used for producing at the predetermined site place of DNA the technology that replaces sudden change and to be those skilled in the art, Clevelaand, OH), the site-directed mutagenesis (Stratagene of QuickChange, San Diego, CA), site-directed mutagenesis or other site-directed mutagenesiss of PCR-mediation.

Derivative

" derivative " comprises such peptide, oligopeptides, polypeptide, wherein compare with the aminoacid sequence of the protein of natural generation form (shown in SEQ ID NO:2), they comprise the interpolation of the amino-acid residue that the amino-acid residue that takes place with non-natural takes place amino acid whose replacement or non-natural.Protein " derivative " also comprises such peptide, oligopeptides, polypeptide; wherein compare with the aminoacid sequence of the natural generation form of polypeptide, they comprise change (glycosylation, acidylate, isoprenylation, phosphorylation, Semen Myristicae acidylate, sulphating etc.) amino-acid residue or non-natural change amino-acid residue of natural generation.Compare with the aminoacid sequence that derivative is originated, this derivative can also comprise and covalently or non-covalently one or more non-aminoacid replacement bases of bonded or the interpolation (for example reporter molecule or other parts) of described aminoacid sequence, as amino-acid residue for promote to detect this derivative bonded reporter molecule and take place with non-natural that the proteinic aminoacid sequence of natural generation compares.

Directly to homologue/collateral line homologue

Directly comprise the evolution notion that is used for describing the gene ancestral relationship to homologue and collateral line homologue.The collateral line homologue is the gene of same species endogenous origin in my late grandfather's gene replication, and is from the different biological genes that originate from species formation to homologue directly.

Structural domain

Term " structural domain " refers to along the sequence alignment result of evolution related protein and at one group of conservative amino acid of specific location.Although the amino acid in other positions can change between homologue, however the amino acid indication of the high conservative of specific location proteinic structure, stability or active aspect may be essential amino acid.Structural domain is because of being identified by the conservative degree of the height in the aligned sequences of protein homology thing family, and they can be as identifying that thing is to determine whether the polypeptide of being discussed belongs to the peptide family of before having identified arbitrarily.

Motif/consensus sequence/label

Term " motif " or " consensus sequence " or " label " refer to short conserved regions in the sequence of evolution related protein.Motif is the high conservative part of structural domain often, but also can only comprise the part of structural domain, maybe can be positioned at (if whole amino acid of motif are positioned at outside the structural domain of definition) outside the conserved domain.

Hybridization

Term as defined herein " hybridization " is the process of the mutual renaturation of homologous complementary nucleotide sequence basically wherein.Crossover process can be carried out in solution fully, and promptly two kinds of complementary nucleic acid all are in the solution.Crossover process also can take place under one of complementary nucleic acid is fixed to the situation of matrix such as magnetic bead, agarose (Sepharose) pearl or any other resin.Crossover process also can be fixed on solid support such as nitrocellulose filter or the nylon membrane or be fixed to by for example photolithography under the situation on the silicate glasses upholder (latter is called nucleic acid array or microarray or is called nucleic acid chip) for example at one of complementary nucleic acid carries out.For hybridization is taken place, usually with nucleic acid molecule thermally denature or chemical modification so that double-stranded unwinding become two strands and/or remove hair clip or other secondary structures from single-chain nucleic acid.

Term " severity " refer to the condition of hybridizing therein.The severity of hybridization is formed by condition such as temperature, salt concn, ionic strength and hybridization buffer to be influenced.Usually, low stringency is chosen as when ionic strength of determining and pH, is lower than particular sequence pyrolysis chain temperature (T _m) about 30 ℃.Medium stringency is that temperature is lower than T at this moment _mAbout 20 ℃ and high stringency be this moment temperature be lower than T _mAbout 10 ℃.High stringency hybridization condition generally is used to separate the hybridization sequences that has high sequence similarity with target nucleic acid sequence.Yet nucleic acid can depart from sequence and because of the degeneracy of the genetic codon substantially the same polypeptide of still encoding.Thereby sometimes may need medium stringency hybridization condition to identify this type of nucleic acid molecule.

T _mBe the temperature when ionic strength of determining and pH, 50% target sequence and the probe hybridization that mates fully under described temperature.T _mThe based composition and the length that depend on solution condition and probe.For example, long sequence is hybridized under comparatively high temps specifically.From being lower than T _mAbout 16 ℃ obtain maximum hybridization speed until 32 ℃.The existence of monovalent cation in hybridization solution reduced the Coulomb repulsion of two nucleic acid interchain, thereby promotes crossbred to form; This effect is tangible (for greater concn, this effect can be ignored) for the na concn up to 0.4M.Methane amide reduces the melting temperature(Tm) of DNA-DNA and DNA-RNA duplex, and every percentage ratio methane amide reduces 0.6-0.7 ℃, and adds 50% methane amide and allow to hybridize at 30-45 ℃, though hybridization speed can reduce.Base-pair mismatch has reduced the thermostability of hybridization speed and duplex.On average and for big probe, every % base mispairing T _mDescend about 1 ℃.The type that depends on crossbred, T _mCan use following equation to calculate:

1) DNA-DNA crossbred (Meinkoth and Wahl, Anal.Biochem., 138:267-284,1984):

T _m=81.5 ℃+16.6xlog ₁₀[Na ⁺] ^a+ 0.41x%[G/C ^b]-500x[L ^c] ^-1-0.61x% methane amide

2) DNA-RNA or RNA-RNA crossbred:

T _m＝79.8+18.5(log ₁₀[Na ⁺] ^a)+0.58(％G/Cb ^b)+11.8(％G/C ^b) ²-820/L ^c

3) few DNA or few RNA ^dCrossbred:

For＜20 Nucleotide: T _m=2 (ln)

For 20-35 Nucleotide: T _m=22+1.46 (ln)

^aOr for other monovalent cations, but only be accurate in the 0.01-0.4M scope.

^bBe accurate in the 30%-75% scope only for %GC.

^cThe length of L=duplex (in base pair).

^dOligo, oligonucleotide; Ln, the useful length of=primer=2 * (G/C number)+(A/T number).

Can for example handle to hybridization buffer and with the RNA enzyme with any control non-specific binding of numerous known technologies with proteinaceous solution closed film, interpolation heterology RNA, heterology DNA and SDS.For the non-homology probe, a series of hybridization can be undertaken by changing one of following condition: (i) reduce renaturation temperature (for example from 68 ℃ to 42 ℃) progressively or (ii) reduce methane amide concentration (for example from 50% to 0%) progressively.Those skilled in the art understand during the hybridization can change and will keep or change the multiple parameter of stringency.

Except that the hybridization condition, the hybridization specificity generally also depends on the function of post-hybridization washing.For removing because of the background due to the non-specific hybridization, sample is with the salts solution washing of dilution.The key factor of this type of washing comprises the ionic strength and the temperature of final washing soln: salt concn is low more and wash temperature is high more, and then Xi Di severity is high more.Wash conditions is generally on the hybridization severity or be lower than hybridization severity and carrying out.Positive hybridization produces the signal that doubles background signal at least.Usually, the suitable stringency that is used for nucleic acid hybridization analysis method or gene amplification detection method as mentioned above.Also can select stricter or more undemanding condition.The technician understands during the washing can change and will keep or change the multiple parameter of stringency.

For example, be used for length and be included in 65 ℃ greater than the typical high stringency hybridization condition of the DNA crossbred of 50 Nucleotide and in 1 * SSC and 50% methane amide, hybridize, wash in 0.3 * SSC at 65 ℃ subsequently in 1 * SSC or at 42 ℃.Be used for length and be included in 50 ℃ greater than the example of the medium stringency hybridization condition of the DNA crossbred of 50 Nucleotide and in 6 * SSC and 50% methane amide, hybridize, wash in 2 * SSC at 50 ℃ subsequently in 4 * SSC or at 40 ℃.The length of crossbred is the expection length of hybrid nucleic acid.When the known nucleic acid hybridization of sequence, can determine crossbred length herein by aligned sequences and the described conserved regions of evaluation.1 * SSC is 0.15M NaCl and 15mM Trisodium Citrate; Hybridization solution and washing soln can comprise 5 * Denhardt reagent, 0.5-1.0%SDS, the fragmentation salmon sperm DNA of 100 μ g/ml sex change, 0.5% trisodium phosphate extraly.

In order to define the purpose of severity level, can be with reference to (2001) MolecularCloning:a laboratory manual such as Sambrook, the third edition, Cold Spring Harbor LaboratoryPress, CSH, New York or with reference to Current Protocols in Molecular Biology, John Wiley﹠amp; Sons, N.Y. (1989 and annual upgrade version).

Gene reorganization/orthogenesis

Consisting of of gene reorganization or orthogenesis: DNA reorganization repeatedly, suitably screening and/or selection have the proteinic nucleic acid of improvement biologic activity or variant (Castle etc., (2004) Science 304 (5674): 1151-4 of its part to produce coding subsequently; United States Patent (USP) 5,811,238 and 6,395,547).

Regulatory element/control sequence/promotor

Term " regulatory element ", " control sequence " and " promotor " all are used interchangeably and mean in a broad sense the modulability nucleotide sequence that can realize that the sequence that is attached thereto is expressed in this article.Term " promotor " refer generally to be positioned at genetic transcription starting point upstream and participate in identification and in conjunction with RNA polymerase and other protein, thereby instruct the nucleic acid control sequence of the transcribed nucleic acid that effectively connects.Aforementioned term comprises from typical eukaryotic gene group gene and (comprising for the required TATA frame of accurate transcripting starting, have or do not have the CCAAT box sequence) in deutero-transcriptional regulatory sequences and replying grow stimulation and/or outside stimulus or with the tissue specificity mode change genetic expression the additional adjustment element (as, upstream activating sequence, enhanser and silencer).This term also comprises the transcriptional regulatory sequences of typical prokaryotic gene, and it can comprise-35 frame sequences and/or-10 frame transcriptional regulatory sequences in the case.Term " regulatory element " also comprises gives, activates or strengthen synthetic fusion molecule or the derivative that nucleic acid molecule is expressed in cell, tissue or organ.

" plant promoter " comprises the regulatory element that mediation encoding sequence section is expressed in vegetable cell.Therefore, plant promoter needs not be plant origin, but can be derived from virus or microorganism, for example from the virus of invasion and attack vegetable cell." plant promoter " also can plant-derived cell, for example comes to use by oneself to treat the nucleotide sequence institute plant transformed expressing and describe in this article in the inventive method.This also is applicable to other " plant " modulability signals, as " plant " terminator.The promotor that is used for the nucleotide sequence upstream of the inventive method can be replaced, be inserted and/or disappearance and being modified by one or more Nucleotide, but do not disturb promotor, open reading-frame (ORF) (ORF) or 3 ' regulatory region such as terminator or functional or active away from other 3 ' regulatory regions of ORF.The activity of promotor also might be because of the modification of this promotor, or thoroughly replaces this promotor and increase by more active promotor even from the promotor of allos biology.For expressing in plant, as mentioned above, nucleic acid molecule must effectively be connected to or comprise suitable promotor, and wherein said promotor is on orthochronous point and with needed space expression pattern expressing gene.

Effectively connect

Term as used in this article " effectively connect " refer to functionally be connected between promoter sequence and the goal gene, to such an extent as to can starting goal gene, promoter sequence transcribes.

Constitutive promoter

" constitutive promoter " refers in the major part of g and D but all during the stage and in the promotor that transcriptional activity is arranged at least one cell, tissue or organ under most of envrionment conditions.Following table 2a provides the example of constitutive promoter.

Table 2a: the example of constitutive promoter

Gene source	Reference
		Actin muscle	??McElroy?et?al，Plant?Cell，2：163-171，1990
??HMGP	??WO?2004/070039
		??CAMV?35S	??Odell?et?al，Nature，313：810-812，1985
??CaMV?19S	??Nilsson?et?al.，Physiol.Plant.100：456-462，1997
		??GOS2	??de?Pater?et?al，Plant?J?Nov；2(6)：837-44，1992，WO??2004/065596
Ubiquitin	??Christensen?et?al，Plant?Mol.Biol.?18：675-689，1992
		The rice cyclophilin	??Buchholz?et?al，Plant?Mol?Biol.25(5)：837-43，1994
Corn H3 histone	??Lepetit?et?al，Mol.Gen.Genet.231：276-285，1992
		Clover H3 histone	??Wu?et?al.?Plant?Mol.Biol.11：641-649，1988
Actin muscle 2	??An?et?al，Plant?J.10(1)；107-121，1996
		??34S?FMV	??Sanger?et?al.，Plant.Mol.Biol.，14，1990：433-443
The Rubisco small subunit	??US?4,962,028
		??OCS	??Leisner(1988)Proc?Natl?Acad?Sci?USA?85(5)：2553
??SAD1	??Jain?et?al.，Crop?Science，39(6)，1999：1696
		??SAD2	??Jain?et?al.，Crop?Science，39(6)，1999：1696
??Nos	??Shaw?et?al.(1984)Nucleic?Acids?Res.12(20)：7831-7846
		The V-ATP enzyme	??WO?01/14572
Super promotor	??WO?95/14098
		The G-box protein	??WO?94/12015

The omnipresence promotor

Institute is in a organized way or activity arranged in the cell basically at biology for the omnipresence promotor.

Grow the modulability promotor

Grow the modulability promotor and during certain growth period or in experience is grown the plant part that changes activity is being arranged.

Inducible promoter

(summary is seen Gatz 1997 to inducible promoter replying chemical, Annu.Rev.PlantPhysiol.Plant Mol.Biol., 48:89-108), the transcripting starting that has induced or increase when environmental stimulus or physical stimulation, maybe can be " stress-inducing ", promptly when being exposed to multiple stress conditions, plant activated, or " pathogen-inducible ", promptly when being exposed to multiple pathogenic agent, plant activated.

Organ specificity/tissue-specific promoter

Organ specificity or tissue-specific promoter can be preferentially start the promotor of transcribing in some organ or tissue such as leaf, root, seed tissue etc.For example, " root-specific promoter " is that advantage ground has the promotor of transcriptional activity in roots of plants, and essentially no activity in any other part of plant is although allow any leakage to express in these other parts of plant.Can only in some cell, start the promotor of transcribing and be called " cell-specific " in this article.

List among the example of the root-specific promoter table 2b below:

Table 2b: the example of root-specific promoter

Gene source	Reference
		??RCc3	??Plant?Mol?Biol.1995?Jan；27(2)：237-48
Arabidopis thaliana PHT1	??Kovama?et?al.，2005；??Mudge?et?al.(2002，Plant?J.31：341)
		Clover phosphoric acid translocator	??Xiao?et?al.，2006
Arabidopis thaliana Pyk10	??Nitz?et?al.(2001)Plant?Sci?161(2)：337-346
		Root expressivity gene	??Tingey?et?al.，EMBO?J.6：1，1987.
The plain inducible genes of tobacco growing	??Van?der?Zaal?et?al.，Plant?Mol.Biol.16，??983，1991.
		'beta '-tubulin	??Oppenheimer，et?al.，Gene?63：87，1988.
The tobacco root-specific gene	??Conkling，et?al.，Plant?Physiol.93：1203，??1990.

Colea G1-3b gene	??United?States?Patent?No.5，401，836
		??SbPRP1	??Suzuki?et?al.，Plant?Mol.Biol.21：109-119，??1993.
??LRX1	??Baumberger?et?al.2001，Genes&Dev.??15：1128
		BTG-26 colea (Brassica napus)	??US?20050044585
LeAMT1 (tomato)	??Lauter?et?al.(1996，PNAS?3：8139)
		The LeNRT1-1 (tomato)	??Lauter?et?al.(1996，PNAS?3：8139)
I class patatin gene (potato)	??Liu?et?al.，Plant?Mol.Biol.153：386-395，??1991.
		KDC1 (Radix Dauci Sativae)	??Downey?et?al.(2000，J.Biol.Chem.??275：39420)
The TobRB7 gene	??W?Song(1997)PhD?Thesis，North??Carolina?State?University，Raleigh，NC??USA
		OsRAB5a (rice)	??Wang?et?al.2002，Plant?Sci.163：273
ALF5 (Arabidopis thaliana)	??Diener?et?al.(2001，Plant?Cell?13：1625)
		NRT2; 1Np (Whiteflower Leadword Root leaf tobacco (N.plumbaginifolia))	??Quesada?et?al.(1997，Plant?Mol.Biol.??34：265)

Seed specific promoters mainly has transcriptional activity in seed tissue, but not necessarily only has transcriptional activity (under the situation of leakage expression) in seed tissue.Seed specific promoters can have activity during seed development and/or seed germination.Seed specific promoters can be endosperm and/or aleuron and/or embryo-specific.Show among below table 2c, d of the example of seed specific promoters (endosperm/aleuron/embryo-specific), e, the f.Other examples of seed specific promoters provide in QingQu and Takaiwa (Plant Biotechnol.J.2,113-125,2004), openly incorporate it into this paper as a reference, just look like complete providing equally.

Table 2c: the example of seed specific promoters

Gene source	Reference
		The seed-specific gene	??Simon?et?al.，Plant?Mol.Biol.5：191，1985；
	??Scofield?et?al.，J.Biol.Chem.262：12202，??1987.；
			??Baszczynski?et?al.，Plant?Mol.Biol.14：633，??1990.
Brazil's nut albumin	??Pearson?et?al.，Plant?Mol.Biol.18：235-245，??1992.
		Legumin	??Ellis?et?al.，Plant?Mol.Biol.10：203-214，??1988.
Gluten (rice)	??Takaiwa?et?al.，Mol.Gen.Genet.208：15-22，??1986；
			??Takaiwa?et?al.，FEBS?Letts.221：43-47，1987.
Zein	??Matzke?et?al?Plant?Mol?Biol，14(3)：323-32??1990
		?napA	??Stalberg?et?al，Planta?199：515-519，1996.
Wheat LMW and HMW glutenin-1	??Mol?Gen?Genet?216：81-90，1989；NAR??17：461-2，1989
		Wheat SPA	??Albani?et?al，Plant?Cell，9：171-184，1997
Wheat α, beta, gamma-gliadine	??EMBO?J.3：1409-15，1984
		Barley Itr1 promotor	??Diaz??et??al.(1995)Mol?Gen?Genet??248(5)：592-8
Barley B1, C, D hordein	??Theor?Appl?Gen?98：1253-62，1999；Plant?J??4：343-55，1993；Mol?Gen?Genet?250：750-60，??1996
		Barley DOF	??Mena?et?al，The?Plant?Journal，116(1)：53-62，??1998
?blz2	??EP99106056.7
		The synthetic promotor	??Vicente-Carbajosa?et?al.，Plant?J.13：??629-640，1998.
Paddy prolamine NRP33	??Wu?et?al，Plant?Cell?Physiology?39(8)

	??885-889，1998
		Rice a-Lysozyme lb-1	??Wu?et?al，Plant?Cell?Physiology?39(8)??885-889，1998
Rice OSH1	??Sato?et?al，Proc.Natl.Acad.Sci.USA，93：??8117-8122，1996
		Rice alpha-globulin REB/OHP-1	??Nakase?et?al.Plant?Mol.Biol.33：513-522，??1997
Rice ADP-glucose pyrophosphorylase	??Trans?Res?6：157-68，1997
		Corn ESR gene family	??Plant?J?12：235-46，1997
Chinese sorghum α-kafirin	??DeRose?et?al.，Plant?Mol.Biol?32：1029-35，??1996
		??KNOX	??Postma-Haarsma?et?al.?Plant?Mol.Biol.??39：257-71，1999
The rice oleosin	??Wu?et?al，J.Biochem.123：386，1998
		Sunflower Receptacle oil albumen	??Cummins?et?al.，Plant?Mol.Biol.19：873-876，??1992
PRO0117, the rice 40S ribosomal protein of inferring	??WO?2004/070039
		PRO0136, the rice alanine aminotransferase	Do not deliver
PRO0147, trypsin inhibitor ITR1 (barley)	Do not deliver
		PRO0151, rice WSI18	??WO?2004/070039
PRO0175, rice RAB21	??WO?2004/070039
		??PRO005	??WO?2004/070039
??PRO0095	??WO?2004/070039
		α-Dian Fenmei (Amy32b)	??Lanahan?et?al，Plant?Cell?4：203-211，1992；??Skriver?et?al，??Proc?Natl?Acad?Sci?USA?88：7266-7270，1991
Kethepsin β-sample gene	??Cejudo?et?al，Plant?Mol?Biol?20：849-856，

	??1992
		Barley Ltp2	??Kalla?et?al.，Plant?J.6：849-60，1994
??Chi26	??Leah?et?al.，Plant?J.4：579-89，1994
		Corn B-Peru	??Selinger?et?al.，Genetics?149；1125-38，1998

Table 2d: endosperm specificity promoter example

Gene source	Reference
		Gluten (rice)	??Takaiwa?et?al.?(1986)Mol?Gen?Genet??208：15-22；??Takaiwa?et?al.(1987)FEBS?Letts.221：43-47
Zein	??Matzke?et?al.，(1990)Plant?Mol?Biol?14(3)：??323-32
		Wheat LMW and HMW glutenin-1	??Colot?et?al.(1989)Mol?Gen?Genet?216：81-90，??Anderson?et?al.(1989)NAR?17：461-2
Wheat SPA	??Albani?et?al.(1997)Plant?Cell??9：171-184
		Wheat gliadin	??Rafalski?et?al.(1984)EMBO?3：1409-15
Barley Itr1 promotor	??Diaz?et?al.(1995)Mol?Gen?Genet??248(5)：592-8
		Barley B1, C, D, hordein	??Cho?et?al.(1999)Theor?Appl?Genet??98：1253-62；??Muller?et?al.(1993)Plant?J?4：343-55；??Sorenson?et?al.(1996)Mol?Gen?Genet??250：750-60
Barley DOF	??Mena?et?al，(1998)Plant?J?116(1)：53-62
		??blz2	??Onate?et?al.(1999)J?Biol?Chem??274(14)：9175-82
Synthetic promoter	??Vicente-Carbajosa?et?al.(1998)Plant?J??13：629-640
		Paddy prolamine NRP33	??Wu?et?al，(1998)Plant?Cell?Physiol?39(8)??885-889
Rice Lysozyme lb-1	??Wu?et?al.(1998)Plant?Cell?Physiol?39(8)

	??885-889
		Rice sphaeroprotein REB/OHP-1	??Nakase?et?al.(1997)Plant?Molec?Biol?33：??513-522
Rice ADP-glucose pyrophosphorylase	??Russell?et?al.(1997)Trans?Res?6：157-68
		Corn ESR gene family	??Opsahl-Ferstad?et?al.(1997)Plant?J??12：235-46
Kafirin	??DeRose?et?al.(1996)Plant?Mol?Biol??32：1029-35

Table 2e: endosperm specificity promoter example:

Gene source	Reference
		Rice OSH1	??Sato?et?al，Proc.Natl.Acad.Sci.USA，93：8117-8122，1996
??KNOX	??Postma-Haarsma?et?al，Plant?Mol.Biol.39：257-71，1999
		??PRO0151	??WO?2004/070039
??PRO0175	??WO?2004/070039
		??PRO005	??WO?2004/070039
??PRO0095	??WO?2004/070039

Table 2f: aleuron specificity promoter example:

Gene source	Reference
		α-Dian Fenmei (Amy32b)	??Lanahan?et?al，Plant?Cell?4：203-211，1992；??Skriver?et?al，Proc?Natl?Acad?Sci?USA??88：7266-7270，1991
Kethepsin β-sample gene	??Cejudo?et?al，Plant?Mol?Biol?20：849-856，1992
		Barley Ltp2	??Kalla?et?al.，Plant?J.6：849-60，1994
?Chi26	??Leah?et?al.，Plant?J.4：579-89，1994
		Corn B-Peru	??Selinger?et?al.，Genetics?149；1125-38，1998

Chlorenchyma specificity promoter as defined herein is mainly to have the promotor of transcriptional activity in chlorenchyma, gets rid of any other part of plant basically, and still allows any leakage expression in these other plant parts.

The example table 2g below that can be used for implementing the chlorenchyma specificity promoter of the inventive method shows.

Table 2g: chlorenchyma specificity promoter example

Gene	Express	Reference
			The corn orthophosphate dikinase	The leaf specificity	??Fukavama?et?al.，2001
The corn Phosphoenolpyruvate carboxylase	The leaf specificity	??Kausch?et?al.，2001
			The rice Phosphoenolpyruvate carboxylase	The leaf specificity	??Liu?et?al.，2003
Rice small subunit Rubisco	The leaf specificity	??Nomura?et?al.，2000
			Rice β expansion albumen EXBP9	Root-specific	??WO?2004/070039
Pigeonpea small subunit Rubisco	The leaf specificity	??Panguluri?et?al.，2005
			Pea RBCS3A	The leaf specificity

Another example of tissue-specific promoter is the meristematic tissue specificity promoter, and it mainly has transcriptional activity in meristematic tissue, gets rid of any other tissue of plant basically, and still allows any leakage expression in these other plant parts.The example table 2h below that can be used for implementing the green mitogenetic tissue-specific promoter of the inventive method shows.

Table 2h: meristematic tissue specificity promoter example

Gene source	Expression pattern	Reference
			Rice OSH1	The branch apical meristem, from the embryo globular stage to the seedling phase	??Sato?et?al.(1996)Proc.Natl.??Acad.Sci.USA，93：??8117-8122
The rice metallothionein(MT)	The meristematic tissue specificity	??BAD87835.1
			??WAK1&WAK?2	Branch and roots and tops meristematic tissue, and in leaf that stretches and sepal	??Wagner&Kohorn(2001)??Plant?Cell?13(2)：303-318

Terminator

Term " terminator " comprises such control sequence, and it is the dna sequence dna at transcription unit's end, sends primary transcript is carried out the signal that 3 ' processing and poly-adenosineization and termination are transcribed.Terminator can be derived from natural gene, from multiple other plant gene or from T-DNA.Terminator to be added can be from for example nopaline synthase or octopine synthase gene or alternatively from the other plant gene or more preferably from any other eukaryotic gene.

Selected marker (gene)/reporter gene

" selected marker ", " selected marker " or " reporter gene " comprise any gene from phenotype to cell that give, wherein at the described gene of described cell inner expression promote to identify and/or to select with nucleic acid construct institute's transfection of the present invention or cell transformed.These marker gene can be identified the successful transfer of nucleic acid molecule by a series of different principle.Suitable mark can be selected from the mark of giving antibiotic resistance or Herbicid resistant, the new metabolism proterties of introducing or allowing visual selection.Selected marker's example comprise the gene of giving antibiotic resistance (as make the nptII of Xin Meisu and kantlex phosphorylation or make the hpt of Totomycin phosphorylation or give to for example bleomycin, Streptomycin sulphate, tsiklomitsin, paraxin, penbritin, gentamicin, Geneticin (Geneticin) (G418), the gene of the resistance of spectinomycin or blasticidin), the gene of conferring herbicide resistance (for example provides

The bar of resistance; The aroA or the gox of glyphosate resistance be provided or give for example gene of the resistance of imidazolone, phosphinothricin or sulfourea) or provide the metabolism proterties gene (as allow plant use seminose as the manA of sole carbon source or utilize xylose isomerase or the anti-nutrition mark such as the 2-deoxyglucose resistance of wood sugar).The expression of visual marker gene causes forming color (for example β-glucuronidase, GUS or beta-galactosidase enzymes substrate coloured with it for example X-Gal), luminous (as luciferin/luciferase system) or fluorescence (green fluorescent protein GFP and derivative thereof).This list is only represented the possible mark of minority.Those skilled in the art are familiar with this type of mark.Depend on biology and system of selection, preferred different mark.

Genetically modified/transgenosis/reorganization

Be the object of the invention, " genetically modified ", " transgenosis " or " reorganization " mean expression cassette, gene construct or carrier that comprises this nucleotide sequence or the biology that transforms with nucleotide sequence of the present invention, expression cassette or carrier with regard to nucleotide sequence, these make up all and produce by recombination method, wherein

(a) coding is used for the nucleic acid sequences to proteins of the inventive method, or

(b) the Genetic Control sequence that effectively is connected with nucleotide sequence of the present invention, promotor for example, or

(c) a) and b).

Be not in its natural genotypic environment or modify by recombination method, be modified with may for example adopt replace, interpolation, disappearance, inversion or insert the form of one or more nucleotide residues.Natural genotypic environment is interpreted as and means natural gene group locus or chromogene seat in the plant of source or that exist in genomic library.Under the situation of genomic library, the natural genotypic environment of nucleotide sequence preferably obtains keeping, and is kept at least in part.This environment is distributed at least one side of nucleotide sequence and has 50bp at least, preferred 500bp at least, especially preferred 1000bp at least, the most preferably sequence length of 5000bp at least.The combination of the natural generation of the corresponding nucleotide sequence of used polypeptide in the natural promoter of the nucleotide sequence of the expression cassette of natural generation-for example and the code book inventive method, as hereinbefore defined-when being subjected to modifying, become transgene expression cassette by non-natural synthetic (" manually ") method (as mutagenic treatment) at this expression cassette.Appropriate method is for example at US 5,565,350 or WO00/15815 in describe.

Be the object of the invention, therefore transgenic plant are interpreted as above and mean the natural gene seat that nucleic acid used in the inventive method is not arranged in described Plant Genome (this nucleotide sequence) that described nucleic acid might homology or the expression of allos ground.Yet as mentioned, although transgenosis also means nucleic acid of the present invention or used in the methods of the invention nucleic acid is in the natural place of this nucleotide sequence in the Plant Genome, yet its sequence is modified for native sequences, and/or the adjusting sequence of described native sequences is modified.Transgenosis is interpreted as preferably to mean in the non-natural locus of nucleic acid of the present invention in genome and expresses that the homology that nucleic acid promptly takes place is expressed or preferred heterogenous expression.Preferred transgenic plant have been mentioned in this article.

Transform

Term " introducing " or " conversion " comprise that exogenous polynucleotide are transferred in the host cell as mentioned in this article, and what the method that no matter is used to transform is.Can follow-up clone's property propagation the plant tissue of (no matter take place or the embryo is taken place) by organ can transform and the whole strain plant that can therefrom regenerate with genetic constructs of the present invention.The concrete tissue of selecting will depend on clone's property proliferating system of the concrete species that can be used for and be suitable for just transforming most.The example organization target comprises leaf dish, pollen, embryo, cotyledon, hypocotyl, megagametophyte, corpus callosum tissue, existing meristematic tissue (for example apical meristem, axillalry bud and root meristematic tissue) and inductive meristematic tissue (for example cotyledon meristematic tissue and hypocotyl meristematic tissue).Polynucleotide can instantaneous or stably be introduced host cell and can keep to nonconformity, for example as plasmid.Alternatively, polynucleotide can be integrated in the host genome.The transformed plant cells that produces can be used for regenerating in the manner known to persons skilled in the art the conversion plant subsequently.

Alien gene is converted into and is called conversion in the Plant Genome.The conversion of plant species is quite conventional technology now.Advantageously, the arbitrary method in several method for transformation can be used for goal gene is introduced suitable ancester cell.Be used for to be used for instantaneous conversion or to be used for stable conversion from the method for plant tissue or the vegetable cell conversion and the plant that regenerates.Method for transformation comprises that the chemical, the dna direct that use liposome, electroporation, increase dissociative DNA to take in are injected to the conversion method and the micro-projective method (microprojection) of plant, particle gun blast technique, use virus or pollen.Method for transformation can be selected from calcium/polyoxyethylene glycol method (Krens, F.A. etc., (1982) Nature 296, the 72-74 that is used for protoplastis; (1987) Plant Mol Biol 8:363-373 such as Negrutiu I); The electroporation of protoplastis ((1985) Bio/Technol 3 such as Shillito R.D., 1099-1102); Micro-injection (Crossway A etc., (1986) Mol.Gen Genet 202:179-185) to vegetable material; The particle bombardment method (Klein TM etc., (1987) Nature 327:70) of DNA or RNA bag quilt, (nonconformity) virus infection method etc.Transgenic plant comprise the genetically modified crops plant, preferably produce by agriculture bacillus mediated conversion method.Favourable method for transformation is the conversion method of in plant (in planta).For this purpose, for example might make Agrobacterium act on the meristematic tissue that plant seed maybe might be inoculated plant with Agrobacterium.To act on complete plant or act on flower primordium at least be particularly advantageous to the verified Agrobacterium suspension that makes conversion according to the present invention.Plant continues subsequently to cultivate that (Clough and Bent, Plant J. (1998) 16,735-743) until the seed that obtains the plant of handling.The method that is used for agriculture bacillus mediated rice conversion comprises the known method that is used for the rice conversion, as those methods of in arbitrary following document, describing: European patent application EP 1198985A1, and Aldemita and Hodges (Planta 199:612-617,1996); Chan etc. (Plant Mol Biol 22 (3): 491-506,1993), Hiei etc. (Plant J 6 (2): 271-282,1994), its disclosure is incorporated herein by reference in this article, as providing fully.Under the situation that corn transforms, (Nat.Biotechnol 14 (6): 745-50 for preferable methods such as Ishida etc., 1996) or Frame etc. (Plant Physiol 129 (1): 13-22,2002) describe, its disclosure is incorporated herein by reference as fully in this article.Described method by way of example mode further by Jenes etc., Techniques for Gene Transfer,: Transgenic Plants, the 1st volume, Engineering and Utilization, editor S.D.Kung and R.Wu, Academic Press (1993) 128-143 and at Potrykus Annu.Rev.PlantPhysiol.Plant Molec.Biol.42 (1991) 205-225) in the description.Nucleic acid to be expressed or construct preferably are cloned into the carrier that is suitable for transforming agrobacterium tumefaciens (Agrobacterium tumefaciens), for example pBin19 (Bevan etc., Nucl.Acids Res.12 (1984) 8711).The Agrobacterium that is transformed by this carrier can be used to transform plant according to known way subsequently, the plant of using for example as model, (Arabidopsis is in scope of the present invention as Arabidopis thaliana, be not considered as crop plants) or crop plants, for example tobacco plant is also cultivated them subsequently by the leaf that soaks abrasive leaf or chopping in Agrobacterium solution in suitable medium.The conversion of plant by agrobacterium tumefaciens for example by

With Willmitzer at Nucl.Acid Res. (1988) 16, Vectors for Gene Transfer in Higher Plants is described in 9877 or especially from F.F.White; At Transgenic Plants, the 1st volume, Engineering and Utilization, editor S.D.Kung and R.Wu, AcademicPress is known in 1993, the 15-38 pages or leaves.

Except transformant cell (its necessary subsequently complete plant of regeneration), also might transform the merismatic cell of plant and special those cells that develop into gamete that transform.In this case, the gamete of conversion is followed natural development of plants process, produces transgenic plant.Therefore, for example the Arabidopis thaliana seed is handled with Agrobacterium and obtain seed from is grown plant, and wherein a certain proportion of described plant is transformed and is genetically modified [Feldman, KA and Marks MD (1987) Mol GenGenet 208:274-289 therefore; Feldmann K (1992).: editor C Koncz, N-H Chua and J Shell, Methods in Arabidopsis Research.Word Scientific, Singapore, 274-289 page or leaf].Alternative method based on remove inflorescence repeatedly and make in the rosette in the heart the excision position and the Agrobacterium incubation of conversion, thereby the seed that transforms can obtain at later time point equally, and (Chang (1994) Plant is J.5:551-558; Katavic (1994) Mol Gen Genet, 245:363-370).Yet especially effective means is the vacuum infiltration method of improvement, as " flower is contaminated " method.Under the situation of Arabidopis thaliana vacuum infiltration method, complete plant is under reduced pressure handled [Bechthold, N (1993) with the Agrobacterium suspension.C R Acad Sci Paris Life Sci, 316:1194-1199], and under the situation of " flower dip method ", of short duration incubation [the Clough of Agrobacterium suspension that the flower tissue and the tensio-active agent of growing handled, SJ und Bent, AF (1998) .The Plant J.16,735-743].Gathered in the crops a certain proportion of transgenic seed in both cases, and these seeds can be distinguished by under aforesaid selection condition, cultivating with the non-transgenic seed.In addition, the stable conversion of plastid is favourable because plastid in most of crop with the heredity of parent mode, reduce or eliminated transgenosis through the pollen flow risk.The conversion of chloroplast gene group generally by at Klaus etc., 2004[Nature Biotechnology 22 (2), 225-229] in the exemplary method realization of being showed.In brief, sequence to be transformed be cloned into together with the selected marker and chloroplast gene group homologous flanking sequence between.These homologous flanking sequences instruct locus specificity to be integrated in the plastom(e).Numerous different plant species having been described plastid transforms and summarizes and can come from Bock (2001) transgenosis plastid (Transgenicplastids in basic research and plant biotechnology) .J Mol Biol.2001 September 21 in fundamental research and Plant Biotechnology; 312 (3): 425-38 or Maliga, P (2003) plastid transformation technology commercialization progress (Progress towards commercialization of plastid transformationtechnology) .Trends Biotechnol.21,20-28.Further the biotechnology progress has been made report with the form of unmarked plastid transformant recently, described unmarked plastid transformant can produce (Klaus etc. by the instantaneous marker gene of integrating altogether, 2004, Nature Biotechnology 22 (2), 225-229).

TILLING

TILLING (genome interior orientation inductive local damage) means and is used for producing and/or identifying the nucleic acid induced-mutation technique, and wherein said nucleic acid encoding has to modify expresses and/or active protein.TILLING also allows to select to carry the plant of this type of mutation variants.These mutation variants may be displayed on the intensity aspect or aspect the position or in the expression (if for example sudden change influence promotor) of improvement aspect the time.These mutation variants can show than showed active higher activity by the gene that is in its natural form.TILLING is with high-density mutagenesis and high-throughput screening method combination.The general step of following in TILLING is: (Redei GP and Koncz C (1992) are at Methods inArabidopsis Research in (a) EMS mutagenesis, Koncz C, Chua NH, Schell J, Singapore writes, World Scientific Publishing Co, the 16-82 page or leaf; Feldmann etc., at Meyerowitz EM, Somerville CR writes (1994), Arabidopsis.Cold Spring HarborLaboratory Press, Cold Spring Harbor, NY, 137-172 page or leaf; Lightner J and Caspar T (1998) be at J Martinez-Zapater, J Salinas editor, Methods onMolecular Biology the 82nd volume .Humana Press, Totowa, NJ, 91-104 page or leaf); (b) DNA of individual prepares and compiles; (c) pcr amplification purpose district; (d) sex change and renaturation are to allow to form heteroduplex; (e) DHPLC, wherein with heteroduplex whether the existence in compiling thing detect and be an extra peak in the color atlas; (f) identify mutated individual; (g) to the order-checking of sudden change PCR product.The method that is used for TILLING is (McCallum etc., (2000) NatBiotechnol 18:455-457 well-known in the art; Summary is seen Stemple (2004) Nat Rev Genet 5 (2): 145-50).

Output

Term " output " but mean the measuring result of economic worth usually, general with specify crop, and area and relevant with the time period.Single plant part based on they number, size and/or weight and directly output is had contribution, or actual output is the every acre yield for certain crop and a year, and this determines divided by the acreage of plantation by ultimate production (comprise results with output assessment).

Increase/improvement/enhancing

Term " increase ", " improvement " or " enhancing " be interchangeable and should on the application's implication, refer to compare at least 5%, 6%, 7%, 8%, 9% or 10%, preferably at least 15% or 20%, more preferably 25%, 30%, 35% or 40% more output and/or growth with control plant as defined herein.

Seed production

The seed production itself that increases can show as following one or more indexs: a) seed biomass (seed gross weight) increases, and this can be based on single seed and/or every strain plant and/or per hectare or acre; B) every strain plant increases spends number; C) (full) seed number of Zeng Jiaing; D) the full rate of the seed of Zeng Jiaing (it is expressed as the ratio between full seed number and the seed sum); E) harvest index of Zeng Jiaing, it is expressed as the ratio that can gather in the crops part (as seed) output and total biomass; And f) thousand seed weight of Zeng Jiaing (TKW), this is from the full seed number and the gross weight extrapolation thereof of counting.The TKW that increases can be because of due to the seed size and/or seed weight that increase, and also can be because of due to the increase of embryo and/or endosperm size.

The increase of seed production also can show as the increase of seed size and/or seed volume.In addition, the increase of seed production itself can self-expression be the increase of seed area and/or seed length and/or seed width and/or seed girth also.The output that increases also can produce the structure of improvement, or can occur because of the structure of improvement.

Plant

Term as used in this article " plant " comprise ancestors and offspring and the plant part of whole strain plant, plant, comprise seed, branch, stem, leaf, root (comprising stem tuber), flower and tissue and organ, wherein every kind of mentioned object comprises goal gene/nucleic acid.Term " plant " also comprise vegetable cell, suspension culture, callus, embryo, meristem zone, gametophyte, sporophyte, pollen and sporule, same every kind of object of mentioning comprises goal gene/nucleic acid.

The plant that is used in particular in the inventive method comprises the whole plants that belong to vegitabilia (Viridiplantae) superfamily, especially monocotyledons and dicotyledons comprise being selected from following feeding or feed beans, ornamental plant, food crop, tree or shrub: maple species (Acer spp.), Actinidia species (Actinidia spp.), Abelmoschus species (Abelmoschus spp.), sisal hemp (Agavesisalana), Agropyron species (Agropyron spp.), the bent grass (Agrostis stolonifera) of crawling, allium species (Allium spp.), Amaranthus species (Amaranthus spp.), Europe beach grass (Ammophila arenaria), pineapple (Ananas comosus), Anona species (Annonaspp.), celery (Apium graveolens), Arachis species (Arachis spp.), Artocarpus Forst species (Artocarpus spp.), officinalis (Asparagus officinalis), Avena species (Avenaspp.) (oat (Avena sativa) for example, wild avena sativa (Avena fatua), than praising oat (Avenabyzantina), Avena fatua var.sativa, hybrid oat (Avena hybrida), carambola (Averrhoa carambola), Ce Sinobambusa (Bambusa sp.), wax gourd (Benincasa hispida), Brazil's chestnut (Bertholletia excelsea), beet (Beta vulgaris), Btassica species (Brassicaspp.) (colea (Brassica napus) for example, overgrown with weeds blue or green species (Brassica rapa ssp.) [canola oil dish, rape (oilseed rape), turnip (turnip rape)]), Cadaba farinosa, tea (Camellia sinensis), Canna generalis Bailey (Canna indica), hemp (Cannabis sativa), Capsicum species (Capsicum spp.), Carex elata, papaya (Carica papaya), carissa macrocarpa (Carissa macrocarpa), hickory species (Carya spp.), safflower (Carthamustinctorius), Castanea species (Castanea spp.), America kapok (Ceiba pentandra), hare's-lettuce (Cichorium endivia), Cinnamomum species (Cinnamomum spp.), watermelon (Citrulluslanatus), Citrus species (Citrus spp.), cocoanut species (Cocos spp.), Coffea species (Coffea spp.), taro (Colocasia esculenta), Africa Firmiana species (Cola spp.), Corchorus (Corchorus sp.), coriander (Coriandrum sativum), Corylus species (Corylus spp.), hawthorn species (Crataegus spp.), Stigma Croci (Crocus sativus), Cucurbita species (Cucurbita spp.), Cucumis species (Cucumis spp.), cynara scolymus belongs to (Cynara spp. species), Radix Dauci Sativae (Daucus carota), acutifoliate podocarpium herb species (Desmodium spp.), longan (Dimocarpus longan), Wild yam species (Dioscorea spp.), Diospyros species (Diospyrosspp.), Echinochloa species (Echinochloa spp.), oil palm belongs to (Elaeis) (oil palm (Elaeisguineensis) for example, America oil palm Elaeis (oleifera)) Finger-millet (Eleusine coracana), Plumegrass (Erianthus sp.), loquat (Eriobotrya japonica), eucalyptus belongs to (Eucalyptus sp.), red young fruit (Eugenia uniflora), Fagopyrum species (Fagopyrum spp.), Fagus species (Fagusspp.), alta fascue (Festuca arundinacea), Fructus Fici (Ficus carica), cumquat species (Fortunella spp.), Fragaria species (Fragaria spp.), ginkgo (Ginkgo biloba), Glycine (Glycine spp.) (soybean for example, soybean (Soja hispida) or soybean (Soja max)), upland cotton (Gossypium hirstum), Helianthus (Helianthus spp.) (for example Sunflower Receptacle (Helianthusannuus)), long tube tawny daylily (Hemerocallis fulva), hibiscus species (Hibiscus spp.), Hordeum (Hordeum spp.) (for example barley (Hordeum vulgare)), sweet potato (Ipomoeabatatas), Juglans species (Juglans spp.), lettuce (Lactuca sativa), Lathyrus species (Lathyrus spp.), Lens culinaris (Lens culinaris), flax (Linum usitatissimum), lichee (Litchi chinensis), Lotus species (Lotus spp.), patola (Luffa acutangula), lupinus species (Lupinus spp.), Luzula sylvatica, tomato belongs to (Lycopersicon spp.) (tomato (Lycopersicon esculentum for example, Lycopersicon lycopersicum, Lycopersicon pyriforme)), sclerderm Macroptilium species (Macrotyloma spp.), Malus species (Malus spp.), recessed edge Malpighia coccigera (Malpighia emarginata), shea (Mammeaamericana), mango (Mangifera indica), cassava species (Manihot spp.), sapota (Manilkara zapota), clover (Medicago sativa), Melilotus suaveolens Ledeb. species (Melilotus spp.), Mentha species (Mentha spp.), awns (Miscanthus sinensis), Momordica species (Momordica spp.), black mulberry (Morus nigra), Musa species (Musa spp.), Nicotiana species (Nicotiana spp.), Olea species (Olea spp.), Opuntia species (Opuntiaspp.), bird foot Macroptilium species (Ornithopus spp.), Oryza (Oryza spp.) (rice for example, broad-leaved rice (Oryza latifolia)), millet (Panicum miliaceum), switchgrass (Panicum virgatum), Purple Granadilla (Passiflora edulis), Selinum pastinaca (Pastinaca sativa), Pennisetum species (Pennisetum sp.), Persea species (Persea spp.), celery (Petroselinum crispum), Phalaris grass (Phalaris arundinacea), Phaseolus species (Phaseolus spp.), timothy grass (Phleumpratense), thorn certain herbaceous plants with big flowers species (Phoenix spp.), south reed (Phragmites australis), Physalis species (Physalis spp.), Pinus species (Pinus spp.), Pistacia vera (Pistacia vera), Pisum species (Pisum spp.), annual bluegrass species (Poa spp.), Populus species (Populusspp.), mesquite grass species (Prosopis spp.), Prunus species (Prunus spp.), Psidium species (Psidium spp.), pomegranate (Punica granatum), European pear (Pyrus commnnis), oak species (Quercus spp.), radish (Raphanus sativus), rheum rhabarbarum (Rheumrhabarbarum), currant species (Ribes spp.), castor-oil plant (Ricinus communis), rubus species (Rubus spp.), saccharum species (Saccharum spp.), Salix species (Salixsp.), Sambucus species (Sambucus spp.), rye (Secale cereale), flax species (Sesamum spp.), sinapsis alba species (Sinapis sp.), Solanum (Solanum spp.) (potato (Solanum tuberosum) for example, red eggplant (Solanum integrifolium) or tomato (Solanumlycopersicum)), dichromatism chinese sorghum (Sorghum bicolor), spinach species (Spinacia spp.), Syzygium species (Syzygium spp.), Tagetes species (Tagetes spp.), tamarind (Tamarindus indica), cocoa tree (Theobroma cacao), Clover species (Trifoliumspp.), Triticosecale rimpaui, Triticum (Triticum spp.) (common wheat (Triticum aestivum) for example, durum wheat (Triticum durum), cylinder wheat (Triticumturgidum), Triticum hybernum, Macha wheat (Triticum macha) (Triticum macha), common wheat (Triticum sativum) or common wheat (Triticum vulgare)), little Flower of Chinese Globeflower (Tropaeolumminus), Flower of Chinese Globeflower (Tropaeolum majus), genus vaccinium species (Vaccinium spp.), tare species (Vicia spp.), Vigna species (Vigna spp.), sweet violet (Viola odorata), Vitis species (Vitis spp.), Zea mays, Zizania palustris, zizyphus species (Ziziphus spp.) or the like.

Detailed Description Of The Invention

I.HARPIN

According to first embodiment, the invention provides the method that strengthens output correlated character in the plant, comprise the expression of nucleic acids of regulating coding Harpin correlation factor G (hereinafter being called " HpaG ") polypeptide in the plant.

The preferred method of regulating the expression of nucleic acids of (preferred increasing) coding HpaG polypeptide is by introduce and express the nucleic acid of coding HpaG polypeptide in plant.

Hereinafter refer to as defined herein HpaG polypeptide about any reference of " protein that is used for the inventive method ".Hereinafter about any reference of " nucleic acid that is used for the inventive method " refer to encode nucleic acid of this type of HpaG polypeptide.Therefore the nucleic acid for the treatment of introduced plant (and can be used for implementing the inventive method) be coding now with any nucleic acid of the protein type described, hereinafter be called " HpaG nucleic acid " or " HpaG gene ".

HpaG polypeptide as defined herein comprises any polypeptide with following feature:

(i) the HpaG peptide sequence of representing with the preferred sequence that increases progressively and SEQ ID NO:2 has at least 35%, 40%, and 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or higher sequence identity; With

(ii) such amino acid is formed, and wherein glycine content is about 13% to about 25%, and glutamine content is about 13% to about 20%, and cysteine content is about 0% to about 1%, and histidine content is about 0% to about 1%, and does not wherein have tryptophane.

Preferably, the length of HpaG polypeptide is about 121 to about 143 amino acid.

Preferably, HpaG albumen also comprises conservative motif 1 (SEQ ID NO:3)

G(G/E/D)(N/E)X(Q/R/P)Q(A/S)GX(N/D)G

Wherein the X on 4 can be arbitrary amino acid, preferred S, and N, P, one of R or Q, and wherein the X on 9 can be arbitrary amino acid, preferred Q, E, one of S or P;

And/or conservative motif 2 (SEQ ID NO:4)

(P/A/V)S(P/Q/A)(F/L/Y)TQ(M/A)LM(H/N/Q)IV(G/M)(E/D/Q)

Randomly, HpaG albumen also has conservative motif 3:

QGISEKQLDQLL

And/or conservative motif 4:

ILQAQN

In addition, HpaG polypeptide (at least in their natural form) in the environmental Cvi-0 of Arabidopis thaliana, cause allergic reaction (Kim et al., J.Bacteriol.185,3155-3166,2003).

Alternatively, the proteic homologue of HpaG has at least 25% by the amino acid that preferred sequence that increases progressively and SEQ ID NO:2 represent, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% overall sequence identity, condition are that this homologous sequence comprises as above-mentioned conservative motif.Overall sequence identity is used the overall comparison algorithm, and (GCG Wisconsin Package, Accelrys) the Needleman Wunsch algorithm in are preferably determined with default parameters as the GAP program.Compare with overall sequence identity, when only considering conserved domain or motif, sequence identity will be higher usually.

Term " structural domain " and " motif " " definition " part definition in the text.There is the specialized database that is used to identify structural domain, as SMART (Schultz etc. (1998) the Proc.Natl.Acad.Sci. U.S. 95,5857-5864; Letunic etc. (2002) Nucleic Acids Res 30,242-244), InterPro (Mulder etc., (2003) Nucl.Acids.Res.31,315-318), Prosite (Bucher and Bairoch (1994), A generalized profile syntax for biomolecular sequencesmotifs and its function in automatic sequence interpretation (be used for the broad sense collection of illustrative plates grammer of biomolecular sequence motif and in the function of automatization sequence interpretation), () ISMB-94; Second molecular biology intelligence system international conference collected works .Altman R., Brutlag D., Karp P., Lathrop R., Searls D. writes, the 53-61 page or leaf, AAAI prints, Menlo Park; Hulo etc., Nucl.Acids.Res.32:D134-D137, (2004)) or Pfam (Bateman etc., Nucleic Acids Research 30 (1): 276-280 (2002)).The one group of instrument that is used for analysing protein sequence on the computer chip can obtain on ExPASY proteomics server (by the trustship (Gasteiger etc. of Switzerland information biology institute, ExPASy:theproteomics server for in-depth protein knowledge and analysis (the protein science server that is used for deep understanding and analysing protein), Nucleic Acids Res.31:3784-3788 (2003)).Structural domain also can use routine techniques as identifying by sequence alignment.

It is well-known in the art being used for the method that aligned sequences is used for comparison, and these class methods comprise GAP, BESTFIT, BLAST, FASTA and TFASTA.GAP uses Needleman and Wunsch algorithm ((1970) J Mol Biol 48:443-453) to find (promptly covering complete sequence) the of overall importance comparison that makes the maximization of coupling number and make minimized two complete sequence of room number.BLAST algorithm (Altschul etc. (1990) J Mol Biol 215:403-10) sequence of calculation identity percentage ratio and execution are to the statistical study of similarity between two sequences.Being used to carry out software that BLAST analyzes and being the public, to pass through NCBI (NCBI) obtainable.Homologue can use for example ClustalW multiple sequence alignment algorithm (1.83 version) evaluation easily to give tacit consent to pairing comparison parameter and methods of marking (with percentage ratio).The overall percentage ratio of similarity and identity also can use one of obtainable method in the MatGAT software package and determine (Campanella etc., BMCBioinformatics.2003 July 10; 4:29.MatGAT: with protein or dna sequence dna and the application that produces similarity/identity matrix).Can carry out trickle craft and write to optimize the comparison between the conservative motif, apparent as those skilled in the art.Except using full length sequence, also can use the ad hoc structure territory in addition to identify homologue.Use program mentioned above, use default parameters, measured sequence identity value at whole nucleotide sequence or amino acid or at structural domain or the conservative motif selected.

The present invention is illustrated by the nucleotide sequence plant transformed of using the coded polypeptide sequence SEQ ID NO:2 that is represented by SEQ ID NO:1, yet enforcement of the present invention is not limited to these sequences.The inventive method can be used as any HpaG-coding nucleic acid that defines in the literary composition or HpaG sample polypeptide and advantageously carry out.

The example of the nucleic acid of coding HpaG polypeptide provides in the Table A of embodiment 1 in the text.This class nucleic acid can be used for implementing method of the present invention.The aminoacid sequence that provides in the Table A of embodiment 1 be the HpaG polypeptide shown in the SEQID NO:2 directly to the example of homologue and collateral line homologue, term " directly to homologue " and " collateral line homologue " such as in the literary composition definition.Other directly can easily be identified by carrying out so-called interactivity blast search to homologue and collateral line homologue.This is usually directed to a BLAST, and a wherein said BLAST comprises with search sequence (for example using the arbitrary sequence of listing in the Table A of embodiment 1) blast search arbitrary sequence database (as public's available ncbi database).When nucleotide sequence begins, generally use BLASTN or TBLASTX (using the standard default value), and, can use BLASTP or TBLASTN (use standard default value) when when protein sequence begins.Randomly can screen BLAST result.The full length sequence of submitting The selection result and non-The selection result subsequently to is with at carry out reverse BLAST (the 2nd BLAST) from the sequence of biology, wherein search sequence is from described biology (be under the situation of SEQ ID NO:1 or SEQ ID NO:2 in search sequence wherein, the 2nd BLAST thereby will be at xanthomonas (Xanthomonas) sequence).The result who compares first and second blast searches subsequently.If from the high-order position of a BLAST hit be derived from search sequence from identical species of deutero-species wherein, oppositely BLAST produces in the highest search sequence of hitting subsequently ideally, then identifies the collateral line homologue; If the high-order position among the blast hit be not derived from search sequence from identical species of deutero-species wherein, and preferably when reverse BLAST, produce the search sequence that belongs to the highest row that hit, then identify directly to homologue.

It is that with low E-value those hit that high-order position is hit.The E-value is low more, mark remarkable more (or in other words, this hit because of the chance odds low more).The calculating of E-value is well-known in the art.Except the E-value, comparative result is also kept the score by identity percentage ratio.Identity percentage ratio refer to two compare identical Nucleotide (or amino acid) number in the length-specific scope between nucleic acid (or polypeptide) sequence.Under the situation of large-scale family, can use ClustalW, use subsequently in abutting connection with the tree method so that help to show clustering and so that identifying of genes involved directly to homologue and collateral line homologue.

The nucleic acid variant also can be used to implement method of the present invention.The example of this type of nucleic acid variant comprises the homologue of the arbitrary aminoacid sequence that provides in the Table A of the embodiment 1 that encodes and the nucleic acid of derivative, term " homologue " and " derivative " such as in the literary composition definition.Also can be used in the inventive method is the straight to the homologue of homologue or collateral line homologue and the nucleic acid of derivative of arbitrary aminoacid sequence of providing in the Table A of coding embodiment 1.Be used for the homologue of the inventive method and derivative and they derived from the protein of unmodified have essentially identical biological activity and functionally active.

The nucleic acid variant of the coding HpaG polypeptide that other nucleic acid variants that can be used for implementing the inventive method comprise the part of the nucleic acid of coding HpaG polypeptide, obtain with the nucleic acid of the nucleic acid hybridization of coding HpaG polypeptide with by gene reorganization.Term hybridization sequences and gene reorganization are as described herein.

The nucleic acid of coding HpaG polypeptide needs not to be total length nucleic acid, does not use the total length nucleotide sequence because the enforcement of the inventive method relies on.According to the present invention, the method that strengthens output correlated character in the plant is provided, be included in the plant part of introducing and expressing any nucleotide sequence that provides in the Table A of embodiment 1, any aminoacid sequence that provides in the Table A of the embodiment 1 that perhaps encodes directly to the part of the nucleic acid of homologue, collateral line homologue or homologue.

The part of nucleic acid can for example prepare by this nucleic acid is produced one or more disappearances.Described part can be used or they can merge with other coding property (or non-coding) sequence with isolating form, so that for example produce several active protein of combination.When merging with other encoding sequences, it is bigger that the gained polypeptide that produces during translation can be compared the polypeptide that this protein portion predicts.

Be used for the HpaG polypeptide that defines in the part coding literary composition of the inventive method, it has the essentially identical biologic activity of aminoacid sequence that provides in the Table A with embodiment 1.Preferably, this part is any part in the nucleic acid that provides in the Table A of embodiment 1, or in the aminoacid sequence that provides in the Table A of embodiment 1 any directly to the part of the coding nucleic acid of homologue or collateral line homologue.The length of this part is preferably at least 70,90,110,130 continuous nucleotides (according to the preferred sequence that increases progressively), wherein said continuous nucleotide is any nucleotide sequence that provides in the Table A of embodiment 1, or any directly to the coding nucleic acid of homologue or collateral line homologue in the aminoacid sequence that provides in the Table A of embodiment 1.Most preferably, this part is the part of nucleic acid sequence SEQ ID NO:1.Preferably, this part encoding amino acid sequence, described peptide sequence comprise any or a plurality of structural domain or the motif that defines in the literary composition.Preferably, this part coded polypeptide sequence, it tends to and the HpaG polypeptide group that comprises the aminoacid sequence shown in the SEQ ID NO:2 when being used for constructing system tree (for example shown in Figure 2), rather than organizes cluster with any other.

Another nucleic acid variant that is used for the inventive method be can under the stringent condition that reduces, preferably under stringent condition with coding as literary composition in the nucleic acid hybridization of defined HpaG polypeptide, or with the nucleic acid of hybridizing as defined part in the literary composition.

The invention provides the method that is used for strengthening the output correlated character plant, its be included in the plant introduce and express can with the nucleic acid of any nucleic acid hybridization of providing in the Table A of embodiment 1, or be included in the plant and introduce and to express nucleic acid like this, its can with any nucleotide sequence of providing in the Table A of embodiment 1 directly to the nucleic acid of the nucleic acid sequence encoding hybridization of homologue, collateral line homologue or homologue.

Be used for the HpaG polypeptide that defines in the hybridization sequences coding literary composition of the inventive method, it has the essentially identical biologic activity of aminoacid sequence that provides in the Table A with embodiment 1.Preferably, hybridization sequences can with arbitrary nucleic acid array hybridizing of providing in the Table A of embodiment 1 or with the part hybridization of arbitrary these sequences, wherein said part as hereinbefore defined, perhaps wherein hybridization sequences can with arbitrary aminoacid sequence of providing in the Table A of embodiment 1 directly to the coding nucleic acid hybridization of homologue or collateral line homologue.Most preferably, hybridization sequences can with as the nucleic acid of SEQ ID NO:1 representative or with its part hybridization.

Preferably, hybridization sequences encoding amino acid sequence, this aminoacid sequence be when being used for tectonic system tree, during as shown in Figure 2 genealogical tree, tend to the HpaG polypeptide group that comprises the aminoacid sequence that SEQ ID NO:2 represents rather than with any other group cluster.

Gene reorganization or orthogenesis also can be used to produce the variant of the nucleic acid of the HpaG polypeptide that coding defines as mentioned; Term " gene reorganization " as defined herein.

According to the present invention, the method that strengthens the output correlated character in plant is provided, be included in the variant of introducing and expressing the arbitrary nucleotide sequence that provides in the Table A of embodiment 1 in the plant, perhaps be included in the straight nucleic acid variant to homologue, collateral line homologue or homologue of introducing in the plant and expressing the arbitrary aminoacid sequence that provides in the Table A of coding embodiment 1, described variant nucleic acid obtains by gene reorganization.

Preferably, the aminoacid sequence of reorganizing the variant nucleic acid encoding that obtains by gene is when being used for the constructing system tree, during as shown in Figure 2 genealogical tree, tend to and the HpaG polypeptide group that comprises the aminoacid sequence shown in the SEQ ID NO:2, rather than with any other group cluster.

In addition, also can obtain the nucleic acid variant by site-directed mutagenesis.Certain methods can be used to realize site-directed mutagenesis, the modal method (Current Protocols inMolecular Biology.Wiley Eds.) that is based on PCR.

The nucleic acid of coding HpaG polypeptide can be from any natural or artificial source.Nucleic acid can be modified from its natural form in composition and/or genome environment by the manual operation of having a mind to.Preferably, HpaG peptide coding nucleic acid is the prokaryotic organism sources, preferably from gram negative bacterium with TTSS, further preferred from plant pathogenetic bacteria with TTSS, more preferably from pseudomonas (Pseudomonaceae) section, further preferably from xanthomonas, most preferably, this nucleic acid is from carpetweed Xanthomonas campestris (Xanthomonas axonopodis).

The enforcement of the inventive method has obtained having the plant of enhanced yield correlated character.Particularly, the enforcement of the inventive method has obtained having with respect to control plant the output of increase, particularly the plant of the seed production of biomass of Zeng Jiaing and/or increase.Term " output " and " seed production " are in " definition " part more detailed description of this paper.

In this article the reference of enhanced yield correlated character is meant biomass (weight) increase of one or more parts of plant, described part can comprise (can gather in the crops) part and/or underground (can gather in the crops) part on the ground.Especially, this type of can gather in the crops part is seed, and the enforcement of the inventive method produces the plant that has the seed production of increase with respect to the seed production of suitable control plant.

With the corn is example, and the output increase can show as following one or more indexs: the increase of the increase of the increase of (establish) plant number of per hectare or acre growth, the increase of every strain plant spike number, line number, every row grain number, grain weight, thousand seed weight, mealie length/diameter, the full rate of seed (wherein the full rate of seed is that the full seed number is total and multiply by 100 divided by seed) and other.With the rice is example, and itself can show as the increase of following one or more indexs the output increase: the increase of per hectare or acre plant number, every strain plant panicle number, every panicle spikelet number, every panicle flower (Xiao Hua) number (it is expressed as the ratio of full seed number to former panicle number), the full rate of seed (wherein the full rate of seed be the full seed number divided by the seed sum and multiply by 100), the increase of thousand seed weight and other.

Method of the present invention has passed through to increase with respect to control plant the method for output, particularly biomass and/or seed production, and this method comprises adjustings, preferably increases the expression of nucleic acids of the HpaG polypeptide as defined herein of encoding in the plant.To notice that viewed output increase is not the result of the biological stress resistance of increase.

Because transgenic plant of the present invention have the output of increase, thereby with respect to the growth velocity of control plant, these plants might show on the corresponding stage in its life cycle increase growth velocity (its life cycle during the small part).Except the output ability that increases, the dietetic alimentation efficient of increase also promotes the increase of output.Observe plant of the present invention and aspect dietetic alimentation, show higher efficient.The dietetic alimentation efficient that increases allows plant to grow better.

The growth velocity that increases can be specific for one or more parts (comprising seed) of plant, or can spread all over whole strain plant basically.Plant with growth velocity of increase can possess short life cycle.The life cycle of plant can be considered as meaning from mature seed and grow to the needed time in stage that plant has produced the dry mature seed similar to parent material.This life cycle can be influenced by following factors, as early stage vigor, growth velocity, green degree index, flowering time and seed maturity speed.The increase of growth velocity can take place during life cycle on the one or more stages in life cycle or in whole plants basically plant.The growth velocity that increases during plant early stage in life cycle can reflect the enhanced vigor.The increase of growth velocity can change the harvest cycle of plant, allows the later sowing of plant and/or than early harvest, otherwise this can not (similar effect can obtain with flowering time early).If growth velocity increases fully, can allow further to sow the seed (for example sow and gather in the crops rice plant, sow and gather in the crops other rice plants subsequently, all processes is all in a conventional growth period) of identical plant species.Similarly, if growth velocity sufficiently increases, can allow further to sow the seed (for example sowing and harvesting corn plant are for example sowed and optional results soybean, potato or any other suitable plant subsequently) of different plant species.The results additional times also is possible in the situation of some crop plants from identical rhizome.The harvest cycle that changes plant can cause the increase of every acre year biomass yield (number of times (as in a year) that can grow and gather in the crops because of any specified plant increases).The increase of growth velocity also can allow cultivating transgenic plant in the geographic area widely than its wild type counterparts, because the region limits of cultivating crop is often determined by the plantation time (season early) or in the adverse environment condition of results period (season in evening).If shorten harvest cycle, then can avoid this class unfavourable condition.Growth velocity can determine that this type of parameter can be by obtain multiple parameter from growth curve: T-Mid (plant reaches the time that its 50% overall dimension spends) and T-90 (plant reaches the time that its 90% overall dimension spends) etc.

According to preferred feature of the present invention, the enforcement of the inventive method produces the plant that has the growth velocity of increase with respect to control plant.Thereby, providing the method that increases plant growth rate according to the present invention, described method comprises adjusting, the preferred expression of nucleic acid in plant that increases defined coding HpaG polypeptide herein.The increase that should be noted that viewed growth velocity is not the result of biological stress resistance.

Compare with control plant, no matter plant is under the non-stress conditions still is that plant is exposed under the multiple abiotic stress, and the increase of output and/or growth velocity all takes place.Plant is generally made and replying being exposed to abiotic stress by growing slowlyer.Under the condition of serious stress of soil condition, plant even can stop growing fully.On the other hand, slightly coerce and be defined as any coercing that plant exposes in this article, the wherein said ability that does not cause plant to stop growing fully and do not recover growth of coercing.Compare with the control plant under the non-stress conditions, slightly coerce and in meaning of the present invention, cause being coerced the plant-growth reduction less than 40%, 35% or 30%, preferably, be more preferably less than 14%, 13%, 12%, 11% or 10% or lower less than 25%, 20% or 15%.Because the progress on the agricultural practice (irrigation, fertilising, pesticide treatments) does not often run into condition of serious stress of soil in the raise crop plant.Therefore, by the agriculture often undesirable characteristic that goes up of the impaired growth of slight stress-inducing.It is that common abiotic (environment) that plant exposes coerced that term " is slightly coerced ".Abiotic stress can because of arid or too much water, anoxic be coerced, due to salt stress, chemical toxicity, oxidative stress and heat, the cold or ice-cold temperature.Abiotic stress can be to coerce (especially because arid), salt stress, oxidative stress or ion by water to coerce the osmotic stress that causes.

The term that defines in the literary composition " abiotic stress " be used in reference to water coerce (because arid or too much water cause), anoxic coerce that (anaerobic stress), salt stress, temperature are coerced (because hot, cold or ice-cold temperature cause), chemical toxicity is coerced with oxidative stress in any one or more coerce.According to an aspect of the present invention, abiotic stress is an osmotic stress, is selected from that water is coerced, salt stress, oxidative stress and ion coerce.Preferably, to coerce be drought stress to water.The term salt stress is not limited to common salt (NaCl), can also be NaCl, KCl, LiCl, MgCl ₂, CaCl ₂Deng in any one or more coercing arbitrarily of causing.

Another example that abiotic environment is coerced is the reduction of one or more required nutrient operabilities of the assimilation of plant-growth and growth.Because nutrientuse efficiency has remarkably influenced to plant biomass and quality product, therefore having toppled over a large amount of fertilizer in the field optimizes plant-growth and quality.The productivity of plant generally is subject to three kinds of main nutrients: phosphorus, potassium and nitrogen, in this three, nitrogen is the speed limit element of plant-growth normally.Therefore, the required main nutrient elements of plant-growth is nitrogen (N).This is the component that is found in the multiple important compound in the viable cell, and described compound comprises amino acid, protein (enzyme), nucleic acid and chlorophyll.About 16% of 1.5% to 2% and total plant protein of plant dry matter is nitrogen.Therefore, the operability of nitrogen is key constraints (Frink etc. (1999) the Proc Natl Acad Sci USA 96 (4): 1175-1180), and also protein accumulation and amino acid composition are had great effect of crop plants growth and production.The crop plants that has the output correlated character (for example seed production) of raising under the limited condition of nitrogen when therefore, cultivating is highly significant.

Biology is coerced normally pathogenic agent, and those that cause as bacterium, virus, fungi, nematode and insect are coerced.

Particularly, method of the present invention can be carried out the plant that has the output of increase with respect to control plant to produce at non-stress conditions or under drought condition.As report in (Planta (2003) 218:1-14) such as Wang, abiotic stress causes influencing unfriendly a series of morphological change of plant-growth and productivity, physiology to change, biological chemistry changes and molecule changes.Known arid, salinity, extreme temperature and oxidative stress are also can damaging and primary cellular defect by induced growth by similar mechanism of connecting each other.Rabbani etc. (Plant Physiol (2003) 133:1755-1767) have described " cross-talk " that drought stress and high salinity are coerced a very high degree.For example, arid and/or salinification mainly show as osmotic stress, cause the destruction of cell homeostasis and ion distribution.Often follow the oxidative stress of high temperature or low temperature, salinity or drought stress can cause functional protein and structural protein sex change.Therefore, these various environment-stress usually activate similar cell signal approach and cell response, as producing stress protein matter, raising antioxidant, accumulation compatible solute and growth-inhibiting.

Term used herein " non-coercing " condition is the envrionment conditions that allows the plant optimum growh.Those skilled in the art know that normal edaphic condition and weather condition for arbitrary given place.

The enforcement of the inventive method is with respect to the appropriate control plant that grows under suitable condition, gives under the non-stress conditions or the output that growing plants increases under the drought stress condition.Thereby according to the present invention, be provided in the method that increases output under the non-stress conditions or under drought condition in the growing plants, described method comprises that the nucleic acid that increases coding HpaG polypeptide expresses in plant.

In addition, the enforcement of the inventive method has obtained plant, and it compares output with increase with the control plant of growing in nutritive deficiency when especially growing under the nitrogen shortage condition under suitable condition.Therefore, according to the present invention, provide the method for output in the growing plants that is increased under the nutritive deficiency condition, this method comprises the expression of nucleic acids that increases coding HpaG polypeptide in the plant.

The enforcement of the inventive method has also obtained plant, it has the plant germination gesture of increase with respect to control plant, especially at development of plants early stage (3,4 weeks after sprouting usually under the situation of rice and corn, but these will be different between species and species), cause early stage vigor.Therefore, according to the present invention, provide the method that increases the early stage vigor of plant, this method comprises adjusting, preferably increases the expression of nucleic acids of coding HpaG polypeptide in the plant.Preferably, realize the increase of seedling vigor by the nucleic acid that is expressed in the coding HpaG under the control of branch specificity promoter.Also provide to produce to have the method for the plant of early stage vigor with respect to control plant, this method comprises adjusting, preferably increases the expression of nucleic acids of coding HpaG polypeptide in the plant.

The plant adaptability that increases for example, also can produce early stage vigor because plant is adapted to their environment (that is, the preferred energy uses and the distribution between branch and root) better.Plant with early stage vigor also demonstrates the seedling survival and the crop of increase and better sets up, it causes highly homogeneous field usually, and (crop grows in the mode of homogeneous, be that most plants arrive different developmental phases basically simultaneously), and cause better and higher output usually.Therefore, early stage vigor can determine by measuring multiple factor, described factor such as thousand seed weight, sprout per-cent, the per-cent that sprouts, growth of seedling, seedling height, root length, root and branch biomass or the like.

The present invention includes the plant or its part (comprising seed) that obtain by method of the present invention.Described plant or its part comprise the nucleic acid transgenosis of coding HpaG polypeptide as hereinbefore defined.

The present invention also provides genetic constructs and carrier to promote to introduce and/or express the nucleic acid of coding HpaG polypeptide in plant.This gene construct can insert the commercially available carrier that is suitable for being converted in the plant and is suitable for expressing goal gene in cell transformed.The present invention also provides as defined gene construct purposes in the methods of the invention in the literary composition.

More specifically, the invention provides construct, it comprises

(a) the coding nucleic acid of HpaG polypeptide of definition as mentioned;

(b) can drive one or more control sequences that the nucleotide sequence of (a) is expressed; Randomly

(c) transcription termination sequence.

Preferably, the HpaG coding nucleic acid is

(i) nucleic acid or its complementary sequence represented of SEQ ID NO:1,

The (ii) coding nucleic acid of HpaG polypeptide of definition as mentioned.

Term " control sequence " and " terminator sequence " are as hereinbefore defined.

Transform plant with the carrier that comprises one of above-mentioned nucleic acid.The known genetic elements that must be present on the carrier of technician is so that successfully transform, select and breed the host cell that contains aim sequence.Aim sequence effectively connects one or more control sequences (promotor at least).

Advantageously, the promotor of arbitrary type no matter be natural or synthetic, may be used to drive the expression of nucleotide sequence.Constitutive promoter or chlorenchyma specificity promoter especially can be used in the described method.See of the definition of " definition " chapters and sections of this paper about multiple promotor type.

Preferably, HpaG nucleic acid or its variant effectively connect to form the type promotor.Effectively constitutive promoter is an also omnipresence expression promoter basically.Further preferably, promotor is from plant, more preferably monocotyledons.Most preferably use GOS2 promotor (from rice) (SEQ ID NO:5).With clear and definite be the HpaG nucleic acid that applicability of the present invention is not limited to SEQ ID NO:1 representative, when by the GOS2 promoters driven, applicability of the present invention also is not limited to the HpaG expression of nucleic acids.Can be used for driving among the table 2a of example in the definitional part of this paper of other constitutive promoters of HpaG expression of nucleic acid and show.

Preferably, constitutive promoter is medium tenacity and has the activity more weak than CaMV 35S promoter.

Alternatively, HpaG nucleic acid or its variant effectively connect the chlorenchyma specificity promoter.Chlorenchyma specificity promoter as defined herein is mainly to have the promotor of transcriptional activity in chlorenchyma, gets rid of any other part of plant basically, and still allows any leakage expression in these other plant parts.The chlorenchyma specificity promoter is protochlorophyllide reductase enzyme promotor preferably, more preferably be substantially similar to the represented protochlorophyllide reductase enzyme promotor of nucleotide sequence of SEQ ID NO:6, most preferably, this promotor such as SEQ ID NO:6 represent.Be noted that application of the present invention is not limited to the represented HpaG coding nucleic acid of SEQ ID NO:1, application of the present invention also is not limited to by the expression of this HpaG coding nucleic acid of protochlorophyllide reductase enzyme promoters driven.The example that also can be used for carrying out other chlorenchyma specificity promoters of the inventive method shows at the definitional part of this paper.

For identifying the promotor of functional equivalent, can analyze the promotor intensity and/or the expression pattern of candidate's promotor, for example promotor effectively is connected in reporter gene, and measures expression level and the pattern of reporter gene in the multiple tissue of plant.Suitable well-known reporter gene comprises for example β-glucuronidase or beta-galactosidase enzymes.Measure promoter activity by the enzymatic activity of measuring β-glucuronidase or beta-galactosidase enzymes.Can compare with promotor intensity and/or expression pattern and with reference to promotor (as promotor used in the inventive method) then.Alternatively, can utilize method well known in the art such as Northern trace in conjunction with the density quantitative analysis of autoradiogram(ARGM), quantitative PCR in real time or RT-PCR, by quantitative mRNA or by the mRNA level of used nucleic acid in the inventive method and the mRNA level of housekeeping gene such as 18S rRNA are compared, measure promotor length (Heid etc., 1996 Genome Methods 6:986-994).Usually, " weak promoter " refers to drive the promotor of encoding sequence low expression level." weak promoter " means about 1/10,000 transcript of each cell to about 1/100,000 transcript, to the level of about 1/500,0000 transcript.On the contrary, " strong promoter " drives the encoding sequence high level expression, and perhaps about 1/10 transcript of each cell is to about 1/100 transcript, to the level of about 1/1,000 transcript.

Randomly, one or more terminator sequences can be used in the construct of introduced plant.Extra regulatory element can comprise transcriptional enhancer and translational enhancer.One skilled in the art will recognize that the terminator sequence and the enhancer sequence that can be suitable in the embodiment of this invention.One skilled in the art will recognize that or can obtain this type of sequence easily.

Intron sequences also can be added on 5 ' non-translational region (UTR) or the encoding sequence, to be increased in the amount of the ripe information that accumulates in the endochylema.But verified montage intron being included in the transcription unit in expression of plants construct and animal expression construct increases genetic expression to reaching 1000 times of (Buchman and Berg Mol.Cell Biol.8:4395-4405 (1988) on mRNA level and the protein level; Callis etc., Gens Dev 1:1183-1200 (1987)).This type of intron enhancement of genetic expression is the strongest generally near being positioned at transcription unit 5 ' end the time.It is known in the art using corn intron A dh1- S introne 1,2 and 6, Bronze-1 intron.For general information, see: The Maiza Hand, the 116th chapter, Freeling and Walbot write, Springer, N.Y. (1994).

Other control sequences (except that promotor, enhanser, silencer, intron sequences, 3 ' UTR and/or 5 ' UTR district) can be protein and/or RNA stable element.One skilled in the art will recognize that or can obtain this type of sequence easily.In addition, the codon that is inserted into the encoding sequence on the construct is selected and can be optimized according to the host cell that will introduce construct.Although genetic code is a degeneracy, biological tendency is in amino acid being used specific cryptosystem rather than this same amino acid being used other codons.The preferred codon option table of multiple biology is known in the art.

Genetic constructs of the present invention can also be included in keeps and/or duplicates required replication orgin sequence in the particular cell types.An example is when needs are maintained additive type genetic elements (for example plasmid or clay molecule) with genetic constructs in bacterial cell.Preferred replication orgin includes, but are not limited to f1-ori and colE1.

For detecting as successful transfer of used nucleotide sequence in the methods of the invention and/or the transgenic plant that selection comprises these nucleic acid, applying marking gene (or reporter gene) is favourable.Thereby genetic constructs can randomly comprise the selected marker.The more detailed selected marker of having described of " definition " part in the literary composition.

When stable the or integration,temporal of known nucleic acid advanced vegetable cell, only a few cell was taken in foreign DNA, and is integrated into its genome (if desired), and this depends on used expression vector and used rotaring dyeing technology.For identifying and select these intasomies, but the gene of the selective marker of will encoding usually (as indicated above those) is introduced in the host cell with goal gene.For example, these marks can use in mutant, and original these genes do not have function in the described mutant, for example lack by ordinary method.In addition, but the used sequence of the nucleic acid of coding selective marker and code book invention polypeptide or the inventive method can be introduced host cell in same carrier, perhaps in independent carrier.By the nucleic acid stability cells transfected of being introduced can be for example by select (for example, but be integrated with the cell survival of selective marker and other cells die) identified.

In case successfully introduce nucleic acid, to no longer need or do not expect to exist in the genetically modified host cell marker gene, particularly microbiotic and herbicide resistance gene advantageously adopt the technology that can remove or excise these marker gene so introduce the method for nucleic acid according to the present invention.A kind of such method is the method that is called cotransformation.The cotransformation method adopts two carriers to transform simultaneously, and a carrier carries according to nucleic acid of the present invention, and second carrier carries marker gene.The transformant of larger proportion receive or for plant, contain (up to 40% or above transformant) two carriers.For Agrobacterium-mediated Transformation, transformant receives only the part of carrier usually, is the sequence of T-DNA institute side joint, and it is often referred to expression cassette.Can from transform plant, remove marker gene by hybridization subsequently.In another approach, utilize the marker gene be integrated into transposon to transform (being called the Ac/Ds technology) with the nucleic acid of expectation.Transformant can be hybridized with the transposase source, perhaps with giving the instantaneous or stable conversion transformant of nucleic acid construct that transposase is expressed.At (about 10%) in some cases, in case successfully transform, transposon is jumped out the host cell gene group and is lost.Under some other situation, transposon skips to different positions.In these cases, must eliminate marker gene by hybridization.In the microbiology field, researched and developed technology that might or be convenient to detect this type of incident.Another advantageous method depends on the method that is called recombination system, it is advantageous that to exempt the hybridization removal process.Foremost this type systematic is called the Cre/lox system.Cre1 is a recombinase, the sequence of its excision between the loxP sequence.If marker gene is incorporated between the loxP sequence, in case successfully transform, because the expression of this recombinase, it is excised.Other recombination systems have HIN/HIX, FLP/FRT and REP/STB system (Tribble etc., J.Biol.Chem., 275,2000:22255-22267; Velmurugan etc., J.CellBiol., 149,2000:553-566).Might be integrated into Plant Genome according to nucleotide sequence of the present invention locus specificity.These methods also can be applied to microorganism such as yeast, fungi or bacterium naturally.

The present invention also provides with respect to control plant, produces the method for the transgenic plant with enhanced yield correlated character, and it is included in any coding nucleic acid of introducing and expressing HpaG polypeptide as hereinbefore defined in the plant.

More specifically, the invention provides to be used for producing and have the enhanced yield correlated character, the method for the transgenic plant of biomass of Zeng Jiaing and/or seed production especially, described method comprises:

(i) coding nucleic acid of introducing and expression HpaG polypeptide in plant or vegetable cell; With

Cell (ii) cultivates plants under the condition that promotes plant-growth and growth.

(i) nucleic acid can be the arbitrary nucleic acid that can encode the HpaG polypeptide as described herein.

Nucleic acid is introduced plant cell or introduced plant itself (comprising any other part of introducing tissue, organ or plant) directly.According to preferred feature of the present invention, nucleic acid is preferably by transforming introduced plant.The more detailed term " conversion " of having described of " definition " part in the literary composition.

The vegetable cell of genetic modification can be regenerated by all methods that those skilled in the art are familiar with.Suitable method be found in S.D.Kung and R.Wu, Potrykus or

Above-mentioned publication with Willmitzer.

Usually after conversion, vegetable cell or the cell colony of selecting one or more marks to exist, wherein said mark become whole strain plant with the material regeneration that transforms subsequently by the effable genes encoding of the plant that moves with the goal gene corotation.In order to select plant transformed, the vegetable material that obtains in conversion is accepted selection condition in principle and is handled, to such an extent as to plant transformed can be distinguished with unconverted plant.For example, can sow with the seed that mode mentioned above obtains, after date when initial the cultivation carries out suitable selection by spraying.Another kind of possibility is included in the seed of growing on the agar plate that uses suitable selective agent (as required after sterilization), makes the seed that only transforms can grow into plant.Perhaps, plant transformed is by the existence screening of above-mentioned those selected markers.

After DNA shifted and regenerates, existence, copy number and/or genome structure that the conversion plant of supposition can for example use Southern to analyze goal gene were estimated.Alternative or extraly, the expression level of newly introducing DNA can use Northern and/or Western to analyze and monitor, and these two kinds of technology are that those skilled in the art are well-known.

The conversion plant that produces can be bred by several different methods, as passing through clonal expansion method or classical breeding technique.For example, the first-generation (or T1) transforms plant can carry out selfing, the s-generation (or T2) transformant that selection is isozygotied, and the T2 plant further breeds by classical breeding technique.

The inverting biological that produces can be taked various ways.For example, they can be the mosaics of transformant and non-transformed cell; Clone's transformant (for example being transformed) to contain whole cells of expression cassette; The transplant of transforming tissue and unconverted tissue (for example in plant) with the root stock of the conversion of unconverted grafting of tender branch.

The present invention extends to any vegetable cell or the plant that produces by any means described in the literary composition clearly, and extends to whole plant parts and propagulum thereof.The present invention extends further to and comprises the former generation conversion that produces by any preceding method or the offspring of transfectional cell, tissue, organ or whole strain plant, unique requirement be the offspring show with by identical one or more yielding characteristicses and/or the phenotypic characteristic of those offsprings that parental generation produced in the inventive method.

The present invention also comprises host cell, and it contains the isolating nucleic acid of coding HpaG polypeptide as hereinbefore defined.The preferred host cell of the present invention is a vegetable cell.Host plant advantageously can synthesize whole plants of used polypeptide in the inventive method in principle for used nucleic acid or carrier, expression cassette or construct or carrier in the inventive method.

The inventive method advantageously is applicable to any plant.

The plant that is used in particular in the inventive method comprises whole plants, especially monocotyledons and the dicotyledons that belongs to vegitabilia's superfamily, comprises feeding or feed beans, ornamental plant, food crop, tree or shrub.According to the preferred embodiment of the invention, plant is a crop plants.The example of crop plants comprises soybean, Sunflower Receptacle, canola oil dish, clover, rape, cotton, tomato, potato and tobacco.Also preferably, plant is a monocotyledons.Monocotyledonous example comprises sugarcane.More preferably, plant is a cereal.The example of cereal comprises rice, corn, wheat, barley, grain, triticale, rye, Chinese sorghum and oat.

The present invention also extend to plant the part gathered in the crops as, but be not limited to seed, leaf, fruit, flower, stem, root stock, stem tuber and bulb.The invention further relates to from oneself, preferably directly from the product in the part gathered in the crops of this type of plant, as dried particles or powder, oil, fat and lipid acid, starch or protein.

The preferable feature according to the present invention, modulated expression are the expression that increases.In this area write up be used to increase the method for nucleic acid or gene or gene product expression and they for example comprise, by the overexpression of suitable promoters driven, use transcriptional enhancer or translational enhancer.Can in the suitable location (generally being the upstream) of the polynucleotide of non-allos form, introduce isolating nucleic acid, so that up-regulated expression as promotor or enhancer element.For example, the endogenous promotor can and/or replace and changes in vivo and (see Kmiec, United States Patent (USP) the 5th, 565, No. 350 by sudden change, disappearance; Zarling etc., PCT/US93/03868), maybe can be with isolating promotor with respect to the correct direction of gene of the present invention and apart from the introduced plant cell, so that controlling gene is expressed.

If need expression of polypeptides, wish usually to comprise poly-adenosine district at the 3 ' end in polynucleotide encoding district.Poly-adenosine district can be from natural gene, from multiple other plant gene or from T-DNA.3 ' terminal sequence to be added can be from for example nopaline synthase or octopine synthase gene or alternatively from another plant gene or more not preferably from any other eukaryotic gene.

The present invention also comprises the nucleic acid of coding HpaG polypeptide as described herein and the purposes that these HpaG polypeptide are used for strengthening plant any above-mentioned output correlated character.

Method of the present invention causes having as described above the plant of enhanced yield correlated character.These proterties also can make up with other favourable economically proterties, and described proterties such as other output strengthen proterties, to other abiotic and biological tolerances of coercing, change the proterties of multiple constitutional features and/or biological chemistry and/or physiological characteristic.

II.SNF2

According to first embodiment, the invention provides the method that in plant, strengthens the output correlated character with respect to control plant, be included in the expression of the nucleotide sequence that increases coding SWI2/SNF2 polypeptide in the plant.

The preferred method of the expression of the nucleotide sequence of increase coding SWI2/SNF2 polypeptide is by introduce and express the nucleotide sequence of coding SWI2/SNF2 polypeptide in plant.

Hereinafter refer to as defined herein SWI2/SNF2 polypeptide about any reference of " protein that is used for the inventive method ".Hereinafter about any reference of " nucleotide sequence that is used for the inventive method " refer to encode nucleotide sequence of this type of SWI2/SNF2 polypeptide.The nucleotide sequence that is inserted into plant (therefore can be used for implementing the inventive method) is the present described type nucleic acid sequences to proteins of coding, and it also is known as " SWI2/SNF2 nucleotide sequence " or " SWI2/SNF2 gene ".

" SWI2/SNF2 polypeptide " as defined herein refers to comprise any polypeptide in ATP enzymatic structure territory, and described structural domain comprises at least 5, preferred 6, more preferably 7, the motif below 8 most preferably from the N-end to the C-end:

(i) the motif I LADDMGLGK (T/S) that represents as SEQ ID NO:103 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif I;

(ii) (V/I/L) (V/I/L) XNW of (A/C) P (T/M/V) S (V/I/L) of the motif IaL (L/V/I) that represents as SEQ ID NO:104 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif Ia;

(iii) (V/I/L) KN of the motif II DEAQ (N/A/H) that represents as SEQ ID NO:105 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif II;

(iv) motif III A (L/M) TGTPXEN that represents as SEQ ID NO:106 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif III;

(v) motif IV (L/I) XF (T/S) Q (F/Y) that represents as SEQ ID NO:107 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif IV;

(vi) (N/T) LTXA (N/S/T) HV of motif VS (L/V) KAGG (V/T/L) G (L/I) that represents as SEQ ID NO:108 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif V;

(the vii) motif Va DRWWNPAVE that represents as SEQ ID NO:109 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif Va; With

(viii) (F/Y) R (I/L) GQ or have at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity of motif VIQA (T/S) DR (A/T/V) that represents as SEQ ID NO:110 with the sequence of the preferred sequence that increases progressively and motif VI

Wherein the X among motif Ia, motif III, motif IV and the motif V is arbitrary amino acid.

Alternatively or extraly, as defined herein " SWI2/SNF2 polypeptide " refers to any peptide sequence, it is when being used for the constructing system tree, (the Flaus et al. (2006) of genealogical tree as shown in Figure 7, mentioned above) time, tend to comprise the peptide sequence shown in the SEQ ID NO:30 the SWI2/SNF2 polypeptide the SSO1653 clade rather than with any other SWI2/SNF2 clade cluster.

Alternatively or extraly, " SWI2/SNF2 polypeptide " as defined herein refers to comprise any peptide sequence in ATP enzymatic structure territory, and described structural domain has at least 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or higher sequence identity with the represented ATP enzymatic structure territory of the SEQ ID NO:111 that comprises among the preferred sequence that increases progressively and the SEQ ID NO:30.

Alternatively or extraly, as defined herein " SWI2/SNF2 polypeptide " refers to any polypeptide, and the arbitrary peptide sequence that provides among SWI2/SNF2 polypeptide that it is represented with the preferred sequence that increases progressively and SEQ ID NO:30 or the table E of this paper has at least 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or higher sequence identity.

Term " structural domain " and " motif " definition in " definition " part of this paper.There is the expert database that is used to identify structural domain, as SMART (Schultz et al. (1998) Proc.Natl.Acad.Sci.USA 95,5857-5864; Letunic et al. (2002) Nucleic Acids Res 30,242-244), InterPro (Mulder et al., (2003) Nucl.Acids.Res.31,315-318), Prosite (Bucher and Bairoch (1994), A generalized profile syntax for biomolecularsequences motifs and its function in automatic sequence interpretation. (In) ISMB-94; Proceedings 2nd International Conference on IntelligentSystems for Molecular Biology.Altman R., Brutlag D., Karp P., LathropR., Searls D., Eds., pp53-61, AAAI Press, Menlo Park; Hulo et al., (2004) Nucl.Acids.Res.32:D134-D137), or Pfam (Bateman et al., (2002) NucleicAcids Research 30 (1): 276-280).The one group of instrument that is used for the Computer Analysis protein sequence can obtain (Swiss Institute of Bioinformatics trustship (Gasteiger et al. on ExPASY protein group server, (2003) ExPASy:the proteomics server for in-depthprotein knowledge and analysis, Nucleic Acids Res 31:3784-3788).Also can use routine techniques, as identifying structural domain by sequence alignment.Provided the analysis of the peptide sequence of SEQ ID NO:30 among the embodiment 9 and 11 below.

It is well-known in the art being used for the method that aligned sequences is used for comparison, and these class methods comprise GAP, BESTFIT, BLAST, FASTA and TFASTA.GAP uses Needleman and Wunsch algorithm ((1970) J Mol Biol 48:443-453) to find (promptly covering complete sequence) the of overall importance comparison that makes the maximization of coupling number and make minimized two complete sequence of room number.BLAST algorithm (Altschul etc. (1990) J Mol Biol 215:403-10) sequence of calculation identity percentage ratio and execution are to the statistical study of similarity between two sequences.Being used to carry out software that BLAST analyzes and being the public, to pass through NCBI (NCBI) obtainable.Homologue can use for example ClustalW multiple sequence alignment algorithm (1.83 version) evaluation easily to give tacit consent to pairing comparison parameter and methods of marking (with percentage ratio).The overall percentage ratio of similarity and identity also can use one of obtainable method in the MatGAT software package and determine (Campanella etc., BMCBioinformatics.2003 July 10; 4:29.MatGAT: with protein or dna sequence dna and the application that produces similarity/identity matrix).Can carry out trickle craft and write to optimize the comparison between the conservative motif, apparent as those skilled in the art.Except using full length sequence, also can use the ad hoc structure territory in addition to identify homologue.Use program mentioned above, use default parameters, at whole nucleotide sequence or peptide sequence (the table F in the literary composition) and/or at the structural domain of selecting (the ATP enzymatic structure territory shown in the SEQ ID NO:111 for example, be included among the SEQ ID NO:30, table F1 in the literary composition) or conservative motif measured sequence identity value, this value provides (in per-cent) in embodiment 3.

The present invention is illustrated by the nucleotide sequence plant transformed of using the coded polypeptide sequence SEQ ID NO:30 that is represented by SEQ ID NO:29, yet enforcement of the present invention is not limited to these sequences.The inventive method can use any SWI2/SNF2-nucleic acid sequence encoding or the SWI2/SNF2 polypeptide as defining in the literary composition advantageously to carry out.

The example of the nucleotide sequence of coded plant SWI2/SNF2 polypeptide provides among the table E of embodiment 8 in the text.This class nucleic acid can be used for implementing method of the present invention.The peptide sequence that provides among the table E of embodiment 8 be the SWI2/SNF2 polypeptide shown in the SEQ ID NO:30 directly to the example of homologue and collateral line homologue, term " directly to homologue " and " collateral line homologue " such as in the literary composition definition.Other directly can easily be identified by carrying out so-called interactivity blast search to homologue and collateral line homologue.This is usually directed to a BLAST, and a wherein said BLAST comprises with search sequence (for example using the arbitrary sequence of listing among the table E of embodiment 8) blast search arbitrary sequence database (as public's available ncbi database).When nucleotide sequence begins, generally use BLASTN or TBLASTX (using the standard default value), and, can use BLASTP or TBLASTN (use standard default value) when when protein sequence begins.Randomly can screen BLAST result.The full length sequence of submitting The selection result and non-The selection result subsequently to is with at carry out reverse BLAST (the 2nd BLAST) from the sequence of biology, wherein search sequence is from described biology (being under the situation of SEQ ID NO:29 or SEQ ID NO:30 in search sequence wherein), perhaps the 2nd BLAST thereby will belong to (Synechocystis) sequence at collection born of the same parents cyanobacteria).The result who compares first and second blast searches subsequently.If from the high-order position of a BLAST hit be derived from search sequence from identical species of deutero-species wherein, oppositely BLAST produces in the highest search sequence of hitting subsequently ideally, then identifies the collateral line homologue; If the high-order position among the blast hit be not derived from search sequence from identical species of deutero-species wherein, and preferably when reverse BLAST, produce the search sequence that belongs to the highest row that hit, then identify directly to homologue.

It is that with low E-value those hit that high-order position is hit.The E-value is low more, mark remarkable more (or in other words, this hit because of the chance odds low more).The calculating of E-value is well-known in the art.Except the E-value, comparative result is also kept the score by identity percentage ratio.Identity percentage ratio refer to two compare identical Nucleotide (or amino acid) number in the length-specific scope between nucleic acid (or polypeptide) sequence.Under the situation of large-scale family, can use ClustalW, use subsequently in abutting connection with the tree method so that help to show clustering and so that identifying of genes involved directly to homologue and collateral line homologue (referring to Fig. 7).

The nucleic acid variant also can be used to implement method of the present invention.The example of this type of variant comprises the homologue of the arbitrary peptide sequence that provides among the table E of the embodiment 8 that encodes and the nucleotide sequence of derivative, and term " homologue " and " derivative " define in this article.Also can be used in the inventive method is the straight to the homologue of homologue or collateral line homologue and the nucleotide sequence of derivative of arbitrary peptide sequence of providing among the table E of coding embodiment 8.Be used for the homologue of the inventive method and derivative and they derived from the protein of unmodified have essentially identical biological activity and functionally active.

Other nucleic acid variants that are used to implement the inventive method comprise the part of the nucleotide sequence of coding SWI2/SNF2 polypeptide, with the allelic variant of the nucleotide sequence of the splice variant of the nucleotide sequence of the nucleotide sequence of the nucleic acid array hybridizing of coding SWI2/SNF2 polypeptide, coding SWI2/SNF2 polypeptide, coding SWI2/SNF2 polypeptide, and the variant of the nucleotide sequence of the coding SWI2/SNF2 polypeptide that obtains by gene reorganization.Term hybridization sequences, splice variant, allelic variant and gene reorganization are as described herein.

The nucleotide sequence of coding SWI2/SNF2 polypeptide needs not be the total length nucleotide sequence, because the enforcement of the inventive method does not rely on the use of using the total length nucleotide sequence.The invention provides the method that in plant, strengthens the seed correlated character, it is included in the plant part of introducing and expressing in the nucleotide sequence that provides among the table E of embodiment 8 any, or any peptide sequence that provides among the table E of embodiment 8 directly to the part of the nucleic acid sequence encoding of homologue, collateral line homologue or homologue.

The part of nucleotide sequence can for example prepare by this nucleotide sequence is produced one or more disappearances.Described part can be used or they can merge with other coding property (or non-coding) sequence with isolating form, so that for example produce several active protein of combination.When merging with other encoding sequences, it is bigger that the gained polypeptide that produces during translation can be compared the polypeptide that this protein portion predicts.

Be used for the SWI2/SNF2 polypeptide that defines in the part coding literary composition of the inventive method, it has the essentially identical biologic activity of peptide sequence that provides among the table E with embodiment 8 (that is, do strengthen the relevant proterties of output).Preferably, this part is any part in the nucleotide sequence that provides in the table E of embodiment 8, or in the peptide sequence that provides among the table E of embodiment 8 any directly to the part of the nucleic acid sequence encoding of homologue or collateral line homologue.The length of this part is preferably at least 1000,1100,1200,1300 or 1400 continuous nucleotides (according to the preferred sequence that increases progressively), wherein said continuous nucleotide is any nucleotide sequence that provides among the table E of embodiment 8, or any directly to the nucleic acid sequence encoding of homologue or collateral line homologue in the peptide sequence that provides among the table E of embodiment 8.Most preferably, this part is the part of nucleic acid sequence SEQ ID NO:29.Preferably, part coded polypeptide sequence, described peptide sequence comprises any or a plurality of structural domain or the motif that defines in the literary composition.Preferably, part coded polypeptide sequence, when it is used for constructing system tree (for example shown in Figure 7), tend to the SSO1653 clade of cluster, rather than cluster is in any other SWI2/SNF2 clade in the SWI2/SNF2 polypeptide that comprises the peptide sequence shown in the SEQ ID NO:30.

Another nucleic acid variant that is used for the inventive method be can under the stringent condition that reduces, preferably under stringent condition with coding as literary composition in the nucleic acid array hybridizing of defined SWI2/SNF2 polypeptide, or with the nucleotide sequence of hybridizing as defined part in the literary composition.

The invention provides the method that is used for strengthening the output correlated character plant, its be included in the plant introduce and express can with the nucleotide sequence of any nucleic acid array hybridizing of providing among the table E of embodiment 8, or be included in the plant and introduce and to express nucleotide sequence like this, its can with any nucleotide sequence of providing among the table E of embodiment 8 directly to the nucleotide sequence of the nucleic acid sequence encoding hybridization of homologue, collateral line homologue or homologue.

Be used for the SWI2/SNF2 polypeptide that defines in the hybridization sequences coding literary composition of the inventive method, it has the essentially identical biologic activity of peptide sequence (that is, strengthening the output correlated character) that provides among the table E with embodiment 8.Preferably, hybridization sequences can with arbitrary nucleic acid array hybridizing of providing among the table E of embodiment 8 or with the part hybridization of arbitrary these sequences, wherein said part as hereinbefore defined, perhaps wherein hybridization sequences can with arbitrary peptide sequence of providing among the table E of embodiment 8 directly to the nucleic acid sequence encoding hybridization of homologue or collateral line homologue.Most preferably, hybridization sequences can with as the nucleotide sequence of SEQ ID NO:29 representative or with its part hybridization.Preferably, hybridization sequences coded polypeptide sequence, described peptide sequence comprises any or a plurality of motif or the structural domain that defines in the literary composition.Preferably, hybridization sequences coded polypeptide sequence, when it is used for constructing system tree (for example shown in Figure 7), tend to the SSO1653 clade of cluster, rather than cluster is in any other SWI2/SNF2 clade in the SWI2/SNF2 polypeptide that comprises the peptide sequence shown in the SEQ ID NO:30.

The another kind of nucleic acid variant that is used for the inventive method is the splice variant of SWI2/SNF2 polypeptide as hereinbefore defined of encoding, splice variant in the literary composition definition.

The invention provides the method that is used for strengthening the output correlated character plant, it is included in the plant splice variant of introducing and expressing any nucleotide sequence that provides among the table E of embodiment 8, or any peptide sequence that provides among the table E of embodiment 8 directly to the splice variant of the nucleic acid sequence encoding of homologue, collateral line homologue or homologue.

The splice variant that is used for the inventive method has the NO:30 with SWI2/SNF2 polypeptide SEQ ID, and the essentially identical biologic activity of any peptide sequence (promptly strengthening the output correlated character) shown in the table E of embodiment 8.Preferably, splice variant coded polypeptide sequence, described peptide sequence comprises any or a plurality of motif or the structural domain that defines in the literary composition.Preferably, splice variant coded polypeptide sequence, when described peptide sequence is used for constructing system tree (for example shown in Figure 7), tend to the SSO1653 clade of cluster, rather than cluster is in any other SWI2/SNF2 clade in the SWI2/SNF2 polypeptide that comprises the peptide sequence shown in the SEQ ID NO:30.

The another kind of nucleic acid variant that is used to implement the inventive method is the allelic variant of nucleotide sequence of SWI2/SNF2 polypeptide as hereinbefore defined of encoding, allelic variant in the literary composition definition.

The invention provides the method that is used for strengthening the output correlated character plant, it is included in the plant allelic variant of introducing and expressing any nucleotide sequence that provides among the table E of embodiment 8, or be included in introduce in the plant and express any peptide sequence of providing among the table E of embodiment 8 directly to the allelic variant of the nucleic acid sequence encoding of homologue, collateral line homologue or homologue.

The allelic variant that is used for the inventive method has the NO:30 with SWI2/SNF2 polypeptide SEQ ID, and the essentially identical biologic activity of any peptide sequence (promptly strengthening the output correlated character) shown in the table E of embodiment 8.The natural existence of allelic variant, and comprise in the methods of the invention be these natural allelic purposes.Preferably, allelic variant is the allelic variant of SEQ ID NO:29, or coding SEQ ID NO:30 directly to the allelic variant of the nucleotide sequence of homologue or collateral line homologue.Preferably, allelic variant coded polypeptide sequence, described peptide sequence comprises any or a plurality of motif or the structural domain that defines in the literary composition.Preferably, allelic variant coded polypeptide sequence, when described peptide sequence is used for constructing system tree (for example shown in Figure 7), tend to the SSO1653 clade of cluster, rather than cluster is in any other SWI2/SNF2 clade in the SWI2/SNF2 polypeptide that comprises the peptide sequence shown in the SEQ ID NO:30.

Gene reorganization or orthogenesis also can be used for producing the variant of the nucleotide sequence of the SWI2/SNF2 polypeptide that coding defines as mentioned; Term " gene reorganization " such as in the literary composition definition.

The invention provides the method that is used for strengthening the output correlated character plant, it is included in the variant of introducing and expressing any nucleotide sequence that provides among the table E of embodiment 8 in the plant, or be included in the plant variant of introducing and expressing nucleotide sequence like this, the any peptide sequence that provides among the table E of wherein said nucleic acid sequence encoding embodiment 8 directly to homologue, collateral line homologue or homologue, described variant nucleic acid sequences obtains by gene reorganization.

The variant nucleic acid sequences of passing through gene reorganization acquisition that is used for the inventive method has the NO:30 with SWI2/SNF2 polypeptide SEQ ID, and the essentially identical biologic activity of any peptide sequence shown in the table E of embodiment 8.Preferably, by the variant nucleic acid sequences coded polypeptide sequence that gene reorganization obtains, described peptide sequence comprises any or a plurality of motif or the structural domain that defines in the literary composition.Preferably, variant nucleic acid sequences coded polypeptide sequence by gene reorganization acquisition, when described peptide sequence is used for constructing system tree (for example shown in Figure 7), tend to the SSO1653 clade of cluster, rather than cluster is in any other SWI2/SNF2 clade in the SWI2/SNF2 polypeptide that comprises the peptide sequence shown in the SEQ IDNO:30.

In addition, the nucleic acid variant also can be by site-directed mutagenic obtained.Several method can be used for realizing site-directed mutagenesis, the method for the modal PCR of being based on (Current Protocols inMolecular Biology.Wiley writes).

The nucleotide sequence of coding SWI2/SNF2 polypeptide can be from any natural or artificial source.Nucleotide sequence can be modified from its natural form on its composition and/or genotypic environment by manual operation.Preferably, the nucleotide sequence of coding SWI2/SNF2 polypeptide is from microbial genome, further preferably from archeobacteria (as from following door: have a liking for the ancient bacterium door (Crenarcheaota) of spring, the ancient bacterium door (Euryarchaeota) of wide area (comprises halobacteriaceae (Halobacteria), methanobacteria family (Methanobacteria), methane coccus guiding principle (Methanococci), methane fire Gammaproteobacteria (Methanopyri), ancient spherical Gammaproteobacteria (Archaeoglobi), pyrogen body guiding principle (Thermoplasmata) and pyrogen body guiding principle (Thermococci)), silicon archeobacteria circle (Korarchaeota) or the ancient Mycota (Nanoarchaeota) of receiving) or bacterium (as from following door: actinomycetes (Actinobacteria), produce water bacterium door (Aquificae), Bacteroidetes (Bacteroidetes)/green bacterium door (Chlorobi), Chlamydiaceae (Chlamydiae), green curved bacterium door (Chloroflexi), pan bacterium door (Chrysiogenetes), cyanobacteria (Cyanobacteria), deferrization bacillus door (Deferribacteres), abnormal cocci belongs to (Deinococcus)-Thermus (Thermus), net group bacterium door (Dictyoglomi), cellulomonas door (Fibrobacteres)/acidfast bacilli door (Acidobacteria), Firmacutes (Firmicutes), fusiform bacilarmature (Fusobacteria), bud Zymomonas mobilis door (Gemmatimonadetes), Lentisphaerae, Nitrospirae, floating mould door (Planctomycetes), protein fungus (Proteobacteria), spirochete (Spirochaetes), thermally desulfurizing bacillus door (Thermodesulfobacteria), hot germ door (Thermomicrobia), thermobacillus door (Thermotogae), wart germ door (Verrucomicrobia), more preferably from cyanobacteria, as synechocystis (Synechocystis sp.), Nostoc (Nostoc sp.), synechococcus belongs to (Synechococcus sp.), proto green algae belongs to (Prochlorococcus sp.), Anaebena sp., Gloeobacter sp., or Thermosynechococcus sp., more preferably from synechocystis, most preferably from cytoalgae PCC6803.

The enforcement of the inventive method has obtained having with respect to control plant the plant of enhanced yield correlated character.

In this article the reference of enhanced yield correlated character is meant biomass (weight) increase of one or more parts of plant, described part can comprise (can gather in the crops) part and/or underground (can gather in the crops) part on the ground.Especially, this type of can gather in the crops part is seed, and the enforcement of the inventive method produces the plant that has the seed production of increase with respect to control plant.

With the corn is example, and the output increase can show as following one or more indexs: the increase of the increase of the increase of (establish) plant number of per hectare or acre growth, the increase of every strain plant spike number, line number, every row grain number, grain weight, thousand seed weight, mealie length/diameter, the full rate of seed (wherein the full rate of seed is that the full seed number is total and multiply by 100 divided by seed) and other.With the rice is example, and itself can show as the increase of following one or more indexs the output increase: the increase of per hectare or acre plant number, every strain plant panicle number, every panicle spikelet number, every panicle flower (Xiao Hua) number (it is expressed as the ratio of full seed number to former panicle number), the full rate of seed (wherein the full rate of seed be the full seed number divided by the seed sum and multiply by 100), the increase of thousand seed weight and other.

The invention provides the method that strengthens the output correlated character of plant with respect to control plant, this method comprises the expression that increases the nucleotide sequence of coding SWI2/SNF2 polypeptide as defined herein in the plant.Preferably, the enhanced yield correlated character be following one or more: (i) the colored number that increases of each panicle; Total seed weight that (ii) every strain plant increases; (iii) (full) seed number of Zeng Jiaing; Or the harvest index that (iv) increases.

Because transgenic plant of the present invention have the enhanced yield correlated character, so these plants may demonstrate the growth velocity (at least at their part of life cycle) of raising with respect to control plant in the growth velocity in the corresponding stage of their life cycles.Except the output ability that increases, the dietetic alimentation efficient of raising also can promote the raising of output.Observe plant of the present invention and in dietetic alimentation, demonstrate higher efficient.The dietetic alimentation efficient that improves allows better plant-growth, no matter plant-growth also is under the non-stress conditions coercing.

The growth velocity that increases can be specific for one or more parts (comprising seed) of plant, or can spread all over whole strain plant basically.Plant with growth velocity of increase can possess short life cycle.The life cycle of plant can be considered as meaning from dry mature seed and grow to the needed time in stage that plant has produced the dry mature seed similar to parent material.This life cycle can be influenced by following factors, as early stage vigor, growth velocity, green degree index, flowering time and seed maturity speed.The increase of growth velocity can take place during life cycle on the one or more stages in life cycle or in whole plants basically plant.The growth velocity that increases during plant early stage in life cycle can reflect the enhanced vigor.The increase of growth velocity can change the harvest cycle of plant, allows the later sowing of plant and/or than early harvest, otherwise this can not (similar effect can obtain with flowering time early).If growth velocity increases fully, can allow further to sow the seed (for example sow and gather in the crops rice plant, sow and gather in the crops other rice plants subsequently, all processes is all in a conventional growth period) of identical plant species.Similarly, if growth velocity sufficiently increases, can allow further to sow the seed (for example sowing and harvesting corn plant are for example sowed and optional results soybean, potato or any other suitable plant subsequently) of different plant species.The results additional times also is possible in the situation of some crop plants from identical rhizome.The harvest cycle that changes plant can cause the increase of every acre year biomass yield (number of times (as in a year) that can grow and gather in the crops because of any specified plant increases).The increase of growth velocity also can allow cultivating transgenic plant in the geographic area widely than its wild type counterparts, because the region limits of cultivating crop is often determined by the plantation time (season early) or in the adverse environment condition of results period (season in evening).If shorten harvest cycle, then can avoid this class unfavourable condition.Growth velocity can determine that this type of parameter can be by obtain multiple parameter from growth curve: T-Mid (plant reaches the time that its 50% overall dimension spends) and T-90 (plant reaches the time that its 90% overall dimension spends) etc.

According to preferred feature of the present invention, the enforcement of the inventive method produces the plant that has the growth velocity of increase with respect to control plant.Thereby, providing the method that increases plant growth rate according to the present invention, described method comprises the expression of nucleotide sequence in plant of the defined coding of increase SWI2/SNF2 polypeptide herein.

Compare with control plant, no matter plant is under the non-stress conditions still is that plant is exposed to multiple coercing down, and the increase of output and/or growth all takes place.Plant is generally replied being exposed to coerce to make by growing slowlyer.Under the condition of serious stress of soil condition, plant even can stop growing fully.On the other hand, slightly coerce and be defined as any coercing that plant exposes in this article, the wherein said ability that does not cause plant to stop growing fully and do not recover growth of coercing.Compare with the control plant that non-stress conditions is grown down, slightly coerce and in meaning of the present invention, cause being coerced the plant-growth reduction less than 40%, 35% or 30%, preferably, be more preferably less than 14%, 13%, 12%, 11% or 10% or lower less than 25%, 20% or 15%.Because the progress on the agricultural practice (irrigation, fertilising, pesticide treatments) does not often run into condition of serious stress of soil in the raise crop plant.Therefore, by the agriculture often undesirable characteristic that goes up of the impaired growth of slight stress-inducing.Slightly coerce is that common biology that plant exposes is coerced and/or abiotic (environment) coerces.Abiotic stress can because of arid or too much water, anoxic be coerced, due to salt stress, chemical toxicity, oxidative stress and heat, the cold or ice-cold temperature.Abiotic stress can be to coerce (especially because arid), salt stress, oxidative stress or ion by water to coerce the osmotic stress that causes.It generally is that those that caused by pathogenic agent such as bacterium, virus, fungi, nematode and insect are coerced that biology is coerced.Term used herein " non-coercing " condition optimization is significantly not exceed the weather of the every day that plant can run into and the envrionment conditions of other abiotic conditions, most preferably, is the envrionment conditions that allows the plant optimum growh.Those skilled in the art know that normal edaphic condition and weather condition for given place.

The enforcement of the inventive method is with respect to the control plant of growing under suitable stress conditions, has enhanced seed correlated character growing plants under the non-stress conditions or under slight drought condition.Thereby according to the present invention, be provided in the method for enhanced seed correlated character in the growing plants under the non-stress conditions or under slight drought condition, described method comprises that increasing the nucleotide sequence of SWI2/SNF2 polypeptide of encoding as defined above expresses in plant.

The enforcement of the inventive method is with respect to the control plant of growing under suitable condition, and growing plants has enhanced seed correlated character under the abiotic stress condition.As report in (Planta (2003) 218:1-14) such as Wang, abiotic stress causes influencing unfriendly a series of morphological change of plant-growth and productivity, physiology to change, biological chemistry changes and molecule changes.Known arid, salinity, extreme temperature and oxidative stress are also can damaging and primary cellular defect by induced growth by similar mechanism of connecting each other.For example, arid and/or salinification mainly show as osmotic stress, cause the destruction of cell homeostasis and ion distribution.Often follow the oxidative stress of high temperature or low temperature, salinity or drought stress can cause functional protein and structural protein sex change.Therefore, these various environment-stress usually activate similar cell signal approach and cell response, as producing stress protein matter, incremental adjustments antioxidant, accumulation compatible solute and growth-inhibiting.Because different environment-stress activates identical path, so the exemplary drought stress of the present invention should not be considered as being confined to drought stress, and should be considered as indicating with respect under suitable stress conditions, general control plant of under the abiotic stress condition, growing, the screening of the degree of participation (involvement) of SWI2/SNF2 polypeptide defined above in enhanced seed correlated character.

Reported between drought stress and high-salt stress especially " cross-talk " of high level (Rabbani etal. (2003) Plant Physiol 133:1755-1767).Therefore, it is evident that, the SWI2/SNF2 polypeptide can be used for strengthening the output correlated character with respect to the control plant of growing under the drought stress condition except them, also can be used for strengthening the output correlated character of plant with respect to the control plant of growing under multiple other abiotic stress conditions.

The term that defines in the literary composition " abiotic stress " is used in reference to that water coerces that (because arid or too much water cause), anoxic coerce that (anaerobic stress) coerces, salt stress, temperature are coerced (because hot, cold or ice-cold temperature cause), chemical toxicity is coerced with oxidative stress in any one or more coerce.According to an aspect of the present invention, abiotic stress is an osmotic stress, is selected from that water is coerced, salt stress, oxidative stress and ion coerce.Preferably, to coerce be drought stress to water.The term salt stress is not limited to common salt (NaCl), can also be NaCl, KCl, LiCl, MgCl ₂, CaCl ₂Deng in any one or more.

Particularly, with respect to the control plant of under suitable stress conditions, growing, one or more below the enhanced yield correlated character can comprise in the growing plants under abiotic stress condition (preferably in the drought stress condition): (i) the over-ground part area of Zeng Jiaing; (ii) total root biomass of Zeng Jiaing; The (iii) thick root biomass of Zeng Jiaing; The (iv) radicula biomass of Zeng Jiaing; (v) each panicle increases spends number; (the vi) full rate of the seed of Zeng Jiaing; (total seed weight that vii) every strain plant increases; (viii) (full) seed number of Zeng Jiaing; Or the harvest index that (ix) increases.

The enforcement of the inventive method has produced the control plant of growing with respect under suitable stress conditions, has the plant of enhanced yield correlated character under the abiotic stress condition.Therefore, according to the present invention, provide the method that strengthens the output correlated character under the abiotic stress condition in the growing plants, this method comprises the expression that increases the nucleotide sequence of coding SWI2/SNF2 polypeptide in the plant.According to an aspect of the present invention, abiotic stress is an osmotic stress, is selected from following one or more: water is coerced, salt stress, oxidative stress and ion are coerced.Preferably, to coerce be drought stress to water.

Another example that abiotic environment is coerced is the reduction of one or more required nutrient operabilities of the assimilation of plant-growth and growth.Because nutrientuse efficiency has remarkably influenced to plant biomass and quality product, therefore having toppled over a large amount of fertilizer in the field optimizes plant-growth and quality.The productivity of plant generally is subject to three kinds of main nutrients: phosphorus, potassium and nitrogen, in this three, nitrogen is the speed limit element of plant-growth normally.Therefore, the required main nutrient elements of plant-growth is nitrogen (N).This is the component that is found in the multiple important compound in the viable cell, and described compound comprises amino acid, protein (enzyme), nucleic acid and chlorophyll.About 16% of 1.5% to 2% and total plant protein of plant dry matter is nitrogen.Therefore, the operability of nitrogen is key constraints (Frink etc. (1999) the Proc Natl Acad Sci USA 96 (4): 1175-1180), and also protein accumulation and amino acid composition are had great effect of crop plants growth and production.The crop plants that has the output of raising under the limited condition of nitrogen when therefore, cultivating is highly significant.

The present invention includes the plant or its part (comprising seed) or the vegetable cell that obtain by method of the present invention.Described plant, plant part or vegetable cell comprise the isolating nucleic acid transgenosis of coding SWI2/SNF2 polypeptide as hereinbefore defined.

The present invention also provides genetic constructs and carrier to promote to introduce and/or express the nucleotide sequence of coding SWI2/SNF2 polypeptide in plant.This gene construct can insert the commercially available carrier that is suitable for being converted in the plant and is suitable for expressing goal gene in cell transformed.The present invention also provides as defined gene construct purposes in the methods of the invention in the literary composition.

More particularly, the invention provides construct, it comprises:

(a) the coding nucleotide sequence of SWI2/SNF2 polypeptide of definition as mentioned;

(b) can drive one or more control sequences that the nucleotide sequence of (a) is expressed; Randomly

(c) transcription termination sequence.

Term " control sequence " and " terminator sequence " are as defined herein.

In one embodiment, one of control sequence of construct is a tissue-specific promoter, is preferred for expanding in early days expression promoter in the tissue.The example that is used for expanding in early days the tissue-specific promoter that tissue expresses is β-expansion protein promoter, for example, and rice β-expansion protein promoter of representing as SEQ ID NO:112.

Plant transforms with the carrier that comprises aforesaid any nucleotide sequence.Those skilled in the art are appreciated that successfully and transform, select and breed the host cell that contains aim sequence and the genetic elements that must exist very much on carrier.Aim sequence effectively is connected with one or more control sequences (at least with promotor).

Advantageously, the promotor of arbitrary type can be used to drive the expression of nucleotide sequence.Promotor can be a constitutive promoter, and it refers at least a cell, tissue or organ at the majority of its g and D but not necessarily in all stages, and have the promotor of transcriptional activity under most envrionment conditionss.Alternatively, promotor can be an inducible promoter, promptly reply chemistry (summary is seen Gatz 1997, Annu.Rev.Plant Physiol.Plant Mol.Biol., 48:89-108), environment or physical stimulation have the transcription initiation of being induced or increasing.Another example of inducible promoter is a stress induced promoter, the promotor that promptly is activated when plant is exposed to multiple stress conditions, perhaps pathogen-inducible promoter.

Additionally or alternatively, promotor can be organ specificity or tissue-specific promoter, promptly can be in some organ or tissues, as preferential initial promotor of transcribing in leaf, root, seed tissue or the like, perhaps promotor can be the omnipresence promotor, in the institute of biology in a organized way or activity is arranged in the cell, perhaps promotor is regulated on can growing basically for it, thereby in some etap or in experience is grown the part of plant of variation activity is arranged.Can in some organ or tissue, initial promotor of transcribing be known as " organ is special " or " tissue-specific " in this article respectively, similarly, can be only in some cell initial promotor of transcribing be known as " cell-specific " in this article.

In one embodiment, coding is the nucleotide sequence of the SWI2/SNF2 polypeptide of definition as mentioned, as the represented nucleotide sequence of SEQ ID NO:29, effectively be connected to tissue-specific promoter, preferably can be preferentially in early days expansion tissue, perhaps in apical meristem, express the promotor of described nucleotide sequence.Preferably, can preferentially expand the express spectra that promotor has Yu β-the expansion protein promoter is suitable of the described nucleotide sequence of tissue expression in early days.More particularly, the promotor that can preferentially expand the described nucleotide sequence of tissue expression in early days is to drive expression promoter in the cell expansion regions of branch or root.More preferably, the promotor that can preferentially expand express nucleic acid sequence in the tissue in early days is β-expansion protein promoter, for example, and the rice β-expansion protein promoter shown in SEQ ID NO:112.

For identifying the promotor of functional equivalent, can analyze the promotor intensity and/or the expression pattern of candidate's promotor, for example promotor effectively is connected in reporter gene, and measures expression level and the pattern of reporter gene in the multiple tissue of plant.Suitable well-known reporter gene comprises for example β-glucuronidase or beta-galactosidase enzymes.Measure promoter activity by the enzymatic activity of measuring β-glucuronidase or beta-galactosidase enzymes.Can compare with promotor intensity and/or expression pattern and with reference to promotor (as promotor used in the inventive method) then.Alternatively, can utilize method well known in the art such as Northern trace in conjunction with the density quantitative analysis of autoradiogram(ARGM), quantitative PCR in real time or RT-PCR, by quantitative mRNA or by the mRNA level of used nucleotide sequence in the inventive method and the mRNA level of housekeeping gene such as 18S rRNA are compared, measure promotor length (Heid etc., 1996 Genome Methods 6:986-994).Usually, " weak promoter " means the promotor that drives the encoding sequence low expression level." weak promoter " means about 1/10,000 transcript of each cell to about 1/100,000 transcript, to the level of about 1/500,0000 transcript.On the contrary, " strong promoter " drives the encoding sequence high level expression, and perhaps about 1/10 transcript of each cell is to about 1/100 transcript, to the level of about 1/1,000 transcript.

Randomly, one or more terminator sequences can be used in the construct of introduced plant.Extra regulatory element can comprise transcriptional enhancer and translational enhancer.One skilled in the art will recognize that the terminator sequence and the enhancer sequence that can be suitable in the embodiment of this invention.One skilled in the art will recognize that or can obtain this type of sequence easily.

Intron sequences also can be added on 5 ' non-translational region (UTR) or the encoding sequence, to be increased in the amount of the ripe information that accumulates in the endochylema.But verified montage intron being included in the transcription unit in expression of plants construct and animal expression construct increases genetic expression to reaching 1000 times of (Buchman and Berg Mol.Cell Biol.8:4395-4405 (1988) on mRNA level and the protein level; Callis etc., Gens Dev 1:1183-1200 (1987)).This type of intron enhancement of genetic expression is the strongest generally near being positioned at transcription unit 5 ' end the time.It is known in the art using corn intron A dh1- S introne 1,2 and 6, Bronze-1 intron.For general information, see: The Maiza Hand, the 116th chapter, Freeling and Walbot write, Springer, N.Y. (1994).

Other control sequences (except that promotor, enhanser, silencer, intron sequences, 3 ' UTR and/or 5 ' UTR district) can be protein and/or RNA stable element.One skilled in the art will recognize that or can obtain this type of sequence easily.

Genetic constructs of the present invention can also be included in keeps and/or duplicates required replication orgin sequence in the particular cell types.An example is when needs are maintained additive type genetic elements (for example plasmid or clay molecule) with genetic constructs in bacterial cell.Preferred replication orgin includes, but are not limited to f1-ori and colE1.

For detecting as successful transfer of used nucleotide sequence in the methods of the invention and/or the transgenic plant that selection comprises these nucleotide sequences, applying marking gene (or reporter gene) is favourable.Thereby genetic constructs can randomly comprise the selected marker.The more detailed selected marker of having described of " definition " part in the literary composition.

When stable the or integration,temporal of known nucleic acid sequence advanced vegetable cell, only a few cell was taken in foreign DNA, and is integrated into its genome (if desired), and this depends on used expression vector and used rotaring dyeing technology.For identifying and select these intasomies, but the gene of the selective marker of will encoding usually (as indicated above those) is introduced in the host cell with goal gene.For example, these marks can use in mutant, and original these genes do not have function in the described mutant, for example lack by ordinary method.In addition, but the used sequence of the nucleotide sequence of coding selective marker and code book invention polypeptide or the inventive method can be introduced host cell in same carrier, perhaps in independent carrier.By the cell of the nucleotide sequence stable transfection of being introduced can be for example by select (for example, but be integrated with the cell survival of selective marker and other cells die) identified.

In case successfully introduce nucleotide sequence, to no longer need or do not expect to exist in the genetically modified host cell marker gene, particularly microbiotic and herbicide resistance gene advantageously adopt the technology that can remove or excise these marker gene so introduce the method for nucleotide sequence according to the present invention.A kind of such method is the method that is called cotransformation.The cotransformation method adopts two carriers to transform simultaneously, and a carrier carries according to nucleotide sequence of the present invention, and second carrier carries marker gene.The transformant of larger proportion receive or for plant, contain (up to 40% or above transformant) two carriers.For Agrobacterium-mediated Transformation, transformant receives only the part of carrier usually, is the sequence of T-DNA institute side joint, and it is often referred to expression cassette.Can from transform plant, remove marker gene by hybridization subsequently.In another approach, utilize the marker gene be integrated into transposon to transform (being called the Ac/Ds technology) with the nucleotide sequence of expectation.Transformant can be hybridized with the transposase source, perhaps with giving the instantaneous or stable conversion transformant of nucleic acid construct that transposase is expressed.At (about 10%) in some cases, in case successfully transform, transposon is jumped out the host cell gene group and is lost.Under some other situation, transposon skips to different positions.In these cases, must eliminate marker gene by hybridization.In the microbiology field, researched and developed technology that might or be convenient to detect this type of incident.Another advantageous method depends on the method that is called recombination system, it is advantageous that to exempt the hybridization removal process.Foremost this type systematic is called the Cre/lox system.Cre1 is a recombinase, the sequence of its excision between the loxP sequence.If marker gene is incorporated between the loxP sequence, in case successfully transform, because the expression of this recombinase, it is excised.Other recombination systems have HIN/HIX, FLP/FRT and REP/STB system (Tribble etc., J.Biol.Chem., 275,2000:22255-22267; Velmurugan etc., J.Cell Biol., 149,2000:553-566).Might be integrated into Plant Genome according to nucleotide sequence of the present invention locus specificity.These methods also can be applied to microorganism such as yeast, fungi or bacterium naturally.

The present invention also provides with respect to control plant, produces the method for the transgenic plant with enhanced yield correlated character, and it is included in any nucleic acid sequence encoding of introducing and expressing SWI2/SNF2 polypeptide as hereinbefore defined in the plant.

More specifically, the invention provides and be used to produce the method that has the transgenic plant of enhanced yield correlated character with respect to control plant, described method comprises:

(i) nucleic acid sequence encoding of introducing and expression SWI2/SNF2 polypeptide in plant or vegetable cell; With

Cell (ii) cultivates plants under the condition that promotes plant-growth and growth.

Nucleotide sequence is introduced plant cell or introduced plant itself (comprising any other part of introducing tissue, organ or plant) directly.According to preferred feature of the present invention, nucleotide sequence is preferably by transforming introduced plant.The more detailed term " conversion " of having described of " definition " part in the literary composition.

The vegetable cell of genetic modification can be regenerated by all methods that those skilled in the art are familiar with.Suitable method be found in S.D.Kung and R.Wu, Potrykus or

Above-mentioned publication with Willmitzer.

Usually after conversion, vegetable cell or the cell colony of selecting one or more marks to exist, wherein said mark become whole strain plant with the material regeneration that transforms subsequently by the effable genes encoding of the plant that moves with the goal gene corotation.In order to select plant transformed, the vegetable material that obtains in conversion is accepted selection condition in principle and is handled, to such an extent as to plant transformed can be distinguished with unconverted plant.For example, can sow with the seed that mode mentioned above obtains, after date when initial the cultivation carries out suitable selection by spraying.Another kind of possibility is included in the seed of growing on the agar plate that uses suitable selective agent (as required after sterilization), makes the seed that only transforms can grow into plant.Perhaps, plant transformed is by the existence screening of above-mentioned those selected markers.

After DNA shifted and regenerates, the conversion plant of supposition can for example use Southern to analyze or quantitative PCR is estimated existence, copy number and/or the genome structure of goal gene.Alternative or extraly, the expression level of newly introducing DNA can use Northern and/or Western to analyze and monitor, and these two kinds of technology are that those skilled in the art are well-known.

The conversion plant that produces can be bred by several different methods, as passing through clonal expansion method or classical breeding technique.For example, the first-generation (or T1) transforms plant can carry out selfing, the s-generation (or T2) transformant that selection is isozygotied, and the T2 plant further breeds by classical breeding technique.

The inverting biological that produces can be taked various ways.For example, they can be the mosaics of transformant and non-transformed cell; Clone's transformant (for example being transformed) to contain whole cells of expression cassette; The transplant of transforming tissue and unconverted tissue (for example in plant) with the root stock of the conversion of unconverted grafting of tender branch.

The present invention extends to any vegetable cell or the plant that produces by any means described in the literary composition clearly, and extends to whole plant parts and propagulum thereof.The present invention extends further to and comprises the former generation conversion that produces by any preceding method or the offspring of transfectional cell, tissue, organ or whole strain plant, unique requirement be the offspring show with by identical one or more yielding characteristicses and/or the phenotypic characteristic of those offsprings that parental generation produced in the inventive method.

The present invention also comprises host cell, and it contains the isolated nucleic acid sequences of coding SWI2/SNF2 polypeptide as hereinbefore defined.The preferred host cell of the present invention is a vegetable cell.Host plant advantageously can synthesize whole plants of used polypeptide in the inventive method in principle for used nucleotide sequence or carrier, expression cassette or construct or carrier in the inventive method.

The inventive method advantageously is applicable to any plant.

The plant that is used in particular in the inventive method comprises whole plants, especially monocotyledons and the dicotyledons that belongs to vegitabilia's superfamily, comprises feeding or feed beans, ornamental plant, food crop, tree or shrub.According to the preferred embodiment of the invention, plant is a crop plants.The example of crop plants comprises soybean, Sunflower Receptacle, canola oil dish, clover, rape, cotton, tomato, potato and tobacco.Also preferably, plant is a monocotyledons.Monocotyledonous example comprises sugarcane.More preferably, plant is a cereal.The example of cereal comprises rice, corn, wheat, barley, grain, rye, triticale, Chinese sorghum and oat.

The present invention also extend to plant the part gathered in the crops as, but be not limited to seed, leaf, fruit, flower, stem, root stock, stem tuber and bulb.The invention further relates to from oneself, preferably directly from the product in the part gathered in the crops of this type of plant, as dried particles or powder, oil, fat and lipid acid, starch or protein.

In this area write up be used to increase the method for nucleotide sequence or gene or gene product expression and they for example comprise, by the overexpression of suitable promoters driven, use transcriptional enhancer or translational enhancer.Can in the suitable location (generally being the upstream) of the polynucleotide of non-allos form, introduce separated nucleic acid sequence, so that up-regulated expression as promotor or enhancer element.For example, the endogenous promotor can and/or replace and changes in vivo and (see Kmiec, United States Patent (USP) the 5th, 565, No. 350 by sudden change, disappearance; Zarling etc., PCT/US93/03868), maybe can be with isolating promotor with respect to the correct direction of gene of the present invention and apart from the introduced plant cell, so that controlling gene is expressed.

If need expression of polypeptides, wish usually to comprise poly-adenosine district at the 3 ' end in polynucleotide encoding district.Poly-adenosine district can be from natural gene, from multiple other plant gene or from T-DNA.3 ' terminal sequence to be added can be from for example nopaline synthase or octopine synthase gene or alternatively from another plant gene or more not preferably from any other eukaryotic gene.

As indicated above, the preferred method of expression that is used for increasing the nucleic acid sequence encoding of SWI2/SNF2 polypeptide is the nucleic acid sequence encoding of introducing and expressing the SWI2/SNF2 polypeptide plant; Yet the effect of realization present method promptly strengthens the output correlated character and also can use other known technologies to realize.It below will be the description of some these type of technology.

A kind of this type of technology is that T-DNA activates mark (Hayashi etc., Science (1992) 1350-1353), it relates in the genome area of goal gene or gene coding region upstream or downstream 10kb sentence structure like this and insert T-DNA (containing promotor (also can be translational enhancer or intron) usually), makes promotor instruct and is decided expression of gene by target.Usually, the promotor control that the natural promoter of deciding gene by target regulating effect that described target is decided genetic expression is destroyed and this gene is in new introducing down.Promotor generally is embedded among the T-DNA.This T-DNA inserts Plant Genome randomly, for example by agroinfection, and causes near the improvement of the gene insertion T-DNA to be expressed.Cause is expressed near the improvement of the gene of the promotor of introducing, the transgenic plant performance dominant phenotype of generation.

Effect of the present invention also can use TILLING (genome interior orientation inductive local damage) technology to produce, and the description of this technology is referring to " definition " part.

Effect of the present invention also can use homologous recombination to produce, and the description of this technology is referring to " definition " part.

The present invention comprises that also the nucleotide sequence of the SWI2/SNF2 polypeptide that coding is as described herein and these SWI2/SNF2 polypeptide are strengthening the purposes in the output correlated character in the plant with respect to control plant.Preferably, the enhanced yield correlated character be following one or more: (i) the colored number that increases of each panicle; Total seed weight that (ii) every strain plant increases; (iii) (full) seed number of Zeng Jiaing; Or the harvest index that (iv) increases.

The present invention also comprises the nucleotide sequence of the SWI2/SNF2 polypeptide that coding is as described herein and these SWI2/SNF2 polypeptide strengthen the output correlated character with respect to the control plant of growing under suitable stress conditions in the growing plants under abiotic stress condition (preferably under the drought stress condition) purposes.Preferably, the enhanced yield correlated character be following one or more: (i) the over-ground part area of Zeng Jiaing; (ii) total root biomass of Zeng Jiaing; The (iii) thick root biomass of Zeng Jiaing; The (iv) radicula biomass of Zeng Jiaing; (v) each panicle increases spends number; (the vi) full rate of the seed of Zeng Jiaing; (total seed weight that vii) every strain plant increases; (viii) (full) seed number of Zeng Jiaing; Or the harvest index that (ix) increases.

The nucleotide sequence or the SWI2/SNF2 polypeptide self of SWI2/SNF2 polypeptide as described herein of encoding can be used for the procedure of breeding, identification of dna mark wherein, and it can be chain with the gene genetic of coding SWI2/SNF2 polypeptide.Described gene/nucleotide sequence or SWI2/SNF2 polypeptide self can be used to define molecule marker.This DNA or protein labeling can be used for the procedure of breeding in order to select to have the plant of the enhanced yield correlated character of definition as mentioned in the methods of the invention.

The allele variant of the gene/nucleotide sequence of coding SWI2/SNF2 polypeptide also can be used for the auxiliary procedure of breeding of mark.This type of procedure of breeding needs to use for example EMS mutagenesis to introduce allelic variation by the mutagenic treatment of plant sometimes; Alternatively, program can begin with the allele variant in one group of non-so-called " natural " source of having a mind to cause.For example begin the evaluation of allele variant then by PCR.Then be select to have the step of super allele variant of the sequence of discussing and the output correlated character that is enhanced.Generally the growth performance that contains the plant of the different allelic variants that sequence is discussed to some extent by monitoring is implemented to select.Can be in the greenhouse or field monitoring growth performance.Other optional step comprise and will identify the plant and the another kind of plant hybridization of excellent allelic variant.This can be used for for example producing target phenotype combination of features.

The nucleotide sequence of coding SWI2/SNF2 polypeptide also can be as probe so that carry out genetic mapping or physical mapping to gene, and described probe reaches the mark of the proterties related with these genes as the part of described gene.This type of information can be used for plant breeding, so that exploitation has the strain system that wants phenotype.The nucleotide sequence that this purposes of the nucleotide sequence of coding SWI2/SNF2 polypeptide only needs to have at least 15 length of nucleotides.The nucleotide sequence of coding SWI2/SNF2 polypeptide can be used as restriction fragment length polymorphism (RFLP) mark.The Southern trace of the plant genome DNA of restrictive diges-tion (Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning, ALaboratory Manual) can be surveyed with the nucleic acid sequence encoding of SWI2/SNF2 polypeptide.What produce carries out genetic analysis to make up genetic map in conjunction with graphic can use a computer subsequently program such as MapMaker (Lander etc. (1987) Genomics 1:174-181).In addition, this nucleotide sequence can be used for surveying the Southern trace of the genomic dna that contains one group of individuality handling through restriction endonuclease, and wherein said one group of individual representative has the parental generation and the offspring of definite genetic cross.The separation of dna polymorphism is marked and is used for the position (Botstein etc. (1980) Am.J.Hum.Genet.32:314-331) of nucleotide sequence in using the previous genetic map that obtains of this colony of calculation code SWI2/SNF2 polypeptide.

Generation and its purposes in genetic mapping of plant gene deutero-probe have been described in Bernatzky and Tanksley (1986) Plant Mol.Biol.Reporter 4:37-41.Numerous publications have been described the genetic mapping that uses methodology mentioned above or its modification method that specific cDNA is cloned.For example, to hand over group, the group that backcrosses, panmictic population, contiguous isozygotying mutually be can be used for mapping with other population of individuals to F2.This type of methodology is that those skilled in the art are well-known.

It (is the arrangement of sequence on physical map that described nucleic acid probe also can be used for physical mapping; See that Hoheisel etc. exists: Non-mammalian Genomic Analyasis:A Practical Guide, Academic press 1996, the 319-346 pages or leaves and the reference of wherein quoting).

In another embodiment, nucleic acid probe can directly use in fluorescence in situ hybridization (FISH) graphing method (Trask (1991) Trends Genet.7:149-154).(several kb are to a hundreds of kb although large-scale clone is used in current FISH graphing method support; See (1995) Genome Res.5:13-20 such as Laan), however the improvement of sensitivity can allow to use shorter probe to carry out the FISH mapping.

The multiple method based on nucleic acid amplification that is used for genetic mapping and physical mapping can be used described nucleotide sequence and implement.Example is found in " definition " part in the literary composition.Example comprises the polymorphism (CAPS of allele specific amplification (Kazazian (1989) J.Lab.Clin.Med 11:95-96), pcr amplified fragment; Sheffield etc., (1993) Genomics 16:325-332), allele-specific connects (Landegren etc., (1988) Science 241:1077-1080), Nucleotide extension (Sokolov (1990) Nucleic Acid Res.18:3671), radiation hybridization mapping (Walter etc., Nat.Genet.7:22-28) and Happy mapping (Dear and Cook, (1989) Nucleic Acid Res.17:6795-6807) (1997).For implementing these methods, it is right to use the nucleotide sequence design and produce the primer that is used for amplified reaction or primer extension reaction.This class primer design is that those skilled in the art are well-known.Use the method for the genetic mapping of PCR-based, may need to identify the difference of leap corresponding to dna sequence dna between the parent of nucleotide sequence of the present invention zone mapping.Yet this is dispensable usually to drawing method.

The inventive method produces to be had as previously described, with respect to control plant, and the plant of enhanced yield correlated character.This proterties also can make up with other favourable economically proterties, increase proterties (normal and coerce under the growth conditions) as other output, to other abiotic stress and biological tolerance of coercing, the proterties of regulating multiple constructivity feature and/or biochemical characteristics and/or physiologic character.

Accompanying drawing is described

The present invention refers now to the following drawings and describes, wherein:

Fig. 1 has shown the comparison of HpaG polypeptide, and the motif 1 of SEQ ID NO:2 and 2 is pointed out with underlining with runic.

Fig. 2 has shown genealogical tree, and it has from other bacteriums and the HpaG polypeptide group of describing from vegetable-protein (different sequences are by the GenBank searching number and/or point out for gi number).

Fig. 3 has shown binary vector, and it is used in the HpaG encoding histone expression of nucleic acids of rice (Oryza sativa) increase from xanthomonas, and described nucleic acid is in rice GOS2 promotor (pGOS2) control down.

Fig. 4 has described the example of the Harpin sequence that can be used for implementing the inventive method in detail.

Fig. 5 has shown the structure iron of the SWI2/SNF2 polypeptide that can be used for implementing the inventive method.The SWI2/SNF2 polypeptide that can be used for implementing the inventive method comprises N-end structure territory and ATP enzymatic structure territory, all is labeled as hollow frame.Typical 8 motif I, Ia, II, III, IV, V, Va and the VI that comprise in the ATP enzymatic structure territory of the SWI2/SNF2 polypeptide that is used for implementing the inventive method are labeled as the black vertical line.

Fig. 6 has shown the al. as Flaus et, the sequence mark in the ATP enzymatic structure territory of 149 SWI2/SNF2SSO1653 subfamily members in (2006).Shown in SEQ ID NO:111 and the ATP enzymatic structure territory that is included among the SEQ ID NO:30 be according to this sequence mark.

Fig. 7 has shown Flaus et al., and the unrooted from the SWI2/SNF2 polypeptide of many SWI2/SNF2 subfamilies of (2006) structure is radial in abutting connection with tree (comprising 149 SWI2/SNF2SSO1653 subfamily members).The polypeptide of representing as SEQ ID NO:30 and all archeobacterias and bacterium (always being called microorganism) SWI2/SNF2 polypeptide are included in the SSO1653 cluster (the figure centre circle is lived).

Fig. 8 shown and Used Defaults, from the CLUSTAL W (1 of the SWI2/SNF2 polypeptide of multiple microorganism; 83) multisequencing comparison.The SWI2/SNF2 polypeptide has sequence conservation among motif I, Ia, II, III, IV, V, Va and the VI in being included in APT enzymatic structure territory basically.These are similarly added frame and evaluation.The another feature that is highlighted is the ATP structural domain, shown in SEQ IDNO:111 and be included among the SEQ ID NO:30.ATP enzymatic structure territory is included between last amino-acid residue of first amino-acid residue of motif 1 of SWI2/SNF2 polypeptide and C-end (from N to the C-end).The beginning in ATP enzymatic structure territory and end are marked, and the black block sign of ATP enzymatic structure territory self through comparison polypeptide top.

Fig. 9 has shown binary vector, and it is used for increasing rice the expression of the cytoalgae PCC6803 nucleotide sequence that is in the coding SWI2/SNF2 polypeptide under β-expansion protein promoter control.

Figure 10 has described the example of the SNF2 sequence that is used to implement the inventive method in detail.

Embodiment

The present invention is described with reference to as an illustration the following example only.The following example is not intended to the thoroughly definition or the restriction scope of the invention.

Embodiment 1: identify the HpaG sequence

Use the database sequence research tool, as basic local comparison instrument (BLAST) (Altschul etc. (1990) J.Mol.Biol.215:403-410; With (1997) Nucleic Acids Res.25:3389-3402 such as Altschul) identify (full-length cDNA, EST or genome) sequence relevant and/or the protein sequence relevant in those sequences of in the Entrez Nucleotide database of NCBI (NCBI), being safeguarded with SEQ ID NO:2 with SEQ ID NO:1.This program is used for relatively and by the significance,statistical that calculates coupling finding the zone that has local similar between sequence by nucleotide sequence or peptide sequence and sequence library.SEQ ID NO:1 encoded polypeptide is used the TBLASTN algorithm, adopt default setting and filtration to offset to ignore the low-complexity sequence.The result who analyzes relatively shows by pairing property, and according to probability scoring (E-value) ordering, wherein is somebody's turn to do the specific comparison result of scoring reflection because of the accidental probability (the E-value is low more, and the significance of hitting is high more) that takes place.Except the E-value, more also keep the score by identity percentage ratio.Identity percentage ratio refer to two compare identical Nucleotide (or amino acid) number in the length-specific scope between nucleic acid (or polypeptide) sequence.In some cases, can adjust default parameters to regulate the severity of search procedure.

Table A provides the nucleic acid relevant with the protein sequence shown in nucleotide sequence shown in the SEQ ID NO:1 and the SEQ ID NO:2 and the tabulation of protein sequence.

Table A: the HpaG nucleic acid sequence encoding and the HpaG polypeptide that are used for the inventive method.

Title	The source is biological	Nucleic acid SEQ ID NO:	Polypeptide SEQ ID NO:	State
					??HpaG	The carpetweed Xanthomonas campestris	??1	??2	Total length
??HpaG_T44C	Synthetic construct	??7	??8	Total length
					??HpaG-T	Synthetic construct	??9	??10	Total length
??Hpa1	Carpetweed Xanthomonas campestris pv.citri str.306	??11	??12	Total length
					??HpaG-N	Synthetic construct	??13	??14	Total length
??HpaG_G	The carpetweed Xanthomonas campestris	??15	??16	Total length
					??Hrp	??Xanthomonas?smithii?subsp.??smithii	??17	??18	Total length
Anaphylaxis functional factor A	Rice Xanthomonas paddy rice mutation (Xanthomonas oryzae pv. Oryzae) the bacterial strain JXOIII that causes a disease	??19	??20	Total length
					??Hpa1	The mutation of causing a disease of rice Xanthomonas paddy rice	??21	??22	Total length
??Hpa1	The mutation of causing a disease of rice Xanthomonas paddy rice	??23	??24	Total length
					??hpaGXooc	The rice Xanthomonas rice mutation (Xanthomonas oryzae pv. Oryzicola) of causing a disease of dwelling	??25	??26	Total length
??Hpa1	Cabbage black rot bacterium wild cabbage mutation (Xanthomonas campestris pv. Campestris) the strains A TCC 33913 that causes a disease	??27	??28	Total length

The comparison of embodiment 2:HpaG peptide sequence

(it is based on clustering algorithm (Clustalalgorithm) (Thompson etc., (1997) Nucleic Acids Res 25:4876-4882 of the progressive comparison of generally using to use the ClustalW program; Chenna etc., (2003) Nucleic Acids Res 31:3497-3500)) carry out the comparison (Fig. 1) of peptide sequence.The default value of the open point penalty in room is 10, and the default value that point penalty is extended in the room is 0.1, and the weight matrix of selection is Blosum 62 (if comparison polypeptide)).

The genealogical tree (Fig. 2) that provides in the AlignX program of use from Vector NTI (Invitrogen) in abutting connection with clustering algorithm structure HpaG polypeptide.

Embodiment 3: be used to implement the calculating of overall identity per-cent (global percentage identity) between the peptide sequence of the inventive method

Being used to implement overall similarity between the full-length polypeptide sequence of the inventive method and identity per-cent utilizes MatGAT (matrix overall comparison instrument) software ((Campanella et al., BMCBioinformatics.20034:29.MatGAT:an application that generatessimilarity/identity matrices using protein or DNA sequences.) determines.MatGAT software need not data are compared in advance, can produce the similarity/identity matrix of DNA or protein sequence.This program is utilized Myers and Miller overall comparison algorithm, and (the open point penalty in room is 12, and to extend point penalty be 2 in the room) carry out a series of comparison in twos, utilize for example Blosum 62 (for polypeptide) calculating similarity and identity, then the result is arranged in distance matrix.Sequence similarity is shown in the diagonal lines Lower Half, and sequence identity is shown in the diagonal lines first half.

More used parameter has:

Matrix: Blosum 62 keeps the score

First room: 12

Extend the room: 2

The overall similarity of peptide sequence length range (the part of polypeptide sequence is foreclosed) and the software analysis of identity the results are shown in table B.The diagonal lines top provides identity per-cent (runic), and the diagonal lines below provides similarity per-cent (normal font).

The identity per-cent that SEQ ID NO:9 and being used to implements between the HpaG peptide sequence of the inventive method can be low to moderate 37% amino acid identity.

Table B: the overall similarity of full-length polypeptide sequence scope and the MatGAT result of identity.

	??1	??2	??3	??4	??5	??6	??7	??8	??9	??10	??11	??12
													??1.SEQ?ID?NO：2		??99.2	??94.0	??91.2	??91.0	??90.2	??85.4	??66.7	??66.7	??66.7	??59.6	??37.7
??2.ABK51589	??99.2		??93.2	??90.5	??90.2	??89.5	??84.7	??67.4	??67.4	??67.4	??60.3	??37.7
													??3.ABK51587	??94.0	??93.2		??85.4	??85.0	??92.0	??79.6	??60.3	??60.3	??60.3	??56.4	??33.3
??4.AAM35307	??92.0	??91.2	??86.1		??82.5	??81.8	??89.8	??70.9	??70.9	??70.9	??61.4	??36.6
													??5.ABK51590	??91.0	??90.2	??90.4	??83.2		??81.2	??76.6	??57.4	??57.4	??57.4	??50.7	??32.8
??6.ABK51588	??90.2	??89.5	??92.0	??82.5	??89.3		??75.2	??58.2	??58.2	??58.2	??56.4	??33.8
													??7.AB636696	??89.5	??88.7	??83.5	??92.7	??80.5	??79.7		??70.7	??70.7	??70.7	??58.8	??37.0
??8.ABJ97680	??77.0	??77.7	??70.5	??80.6	??67.6	??68.3	??81.3		??100.0	??100.0	??64.5	??35.0
													??9.AAC95121	??77.0	??77.7	??70.5	??80.6	??67.6	??68.3	??81.3	??100.0		??100.0	??64.5	??35.0
??10.BAD29979	??77.0	??77.7	??70.5	??80.6	??67.6	??68.3	??81.3	??100.0	??100.0		??64.5	??35.0
													??11.ABB72197	??72.9	??73.7	??72.8	??73.7	??68.0	??72.8	??72.9	??72.7	??72.7	??72.7		??34.6
??12.AAM40538	??51.9	??51.9	??48.0	??49.6	??46.3	??50.4	??50.4	??45.3	??45.3	??45.3	??53.6

Embodiment 4: clone and vector construction

Unless stated otherwise, recombinant DNA technology is according to (Sambrook (2001) MolecularCloning:a laboratory manual, third edition Cold Spring Harbor LaboratoryPress, CSH, New York) in or Ausubel etc. (1994), Current Protocols inMolecular Biology, the standard method of describing in Current Protocols the 1st and 2 volumes is carried out.The standard material and the method that are used for plant molecular work are described at the Plant Molecular Biology Labfax (1993) of the R.D.D.Croy that is published by BIOS Scientific PublicationsLtd (UK) and Blackwell Scientific Publications (UK).

By PCR from carpetweed Xanthomonas campestris DNA amplified library xanthomonas HpaG encoding sequence.Use standard technique, the PCR fragment of purifying expection length also is cloned in subsequently

In the carrier.The clone that enters who comprises SEQ ID NO:1 is used for the LR reaction then, is used for the purpose carrier that rice transforms.This carrier contains as functional element on the T-DNA border: plant selectable marker; The selection markers expression cassette; Be used for expection and be cloned in the purpose nucleotide sequence that enters the clone and carry out the Gateway box of recombinating in the LR body.The rice GOS2 promotor (SEQ ID NO:5) that is used for constitutive expression is positioned at the upstream of this Gateway box.Alternatively, the chlorenchyma specificity promoter also is same useful as protochlorophyllide reductase enzyme promotor (SEQ ID NO:6).

After the LR reconstitution steps, the expression vector pGOS2::HpaG with gained is transformed among the agrobacterium strains LBA4044 according to method well known in the art.

Embodiment 5: Plant Transformation

Rice transforms

The Agrobacterium that contains expression vector is used for transforming rice plant.Ripe dry seed shelling with the Japanese Cultivar Nipponbare of rice.By incubation in 70% ethanol one minute, in 2,%Hg,Cl2 30 minutes subsequently, subsequently with sterile distilled water washing 6 times 15 minutes and implement sterilization.The disinfectant seed is containing 2 subsequently, and the substratum of 4-D (callus inducing medium) is gone up and sprouted.Incubation is after 4 weeks in the dark, scultellum deutero-embryogenic callus is downcut and breeds on a kind of substratum.After 2 weeks, callus by breeding or breed uploading with a kind of substratum to be commissioned to train to support in other 2 weeks.The embryogenic callus sheet is uploaded to be commissioned to train at fresh culture and was supported 3, cultivates (to strengthen the cell fission activity) afterwards altogether.

The agrobacterium strains LBA4404 that contains expression vector is used for common cultivation.Agrobacterium is seeded in to contain on the suitable antibiotic AB substratum and at 28 ℃ and cultivated 3.Subsequently bacterium is collected and is resuspended in liquid and cultivate altogether in the substratum to density (OD600) about 1.Suspension is transferred to culture dish subsequently and callus was soaked 15 minutes in this suspension.Callus is organized subsequently and to be cultivated on the substratum altogether and in the dark in 25 ℃ of incubations 3 days blotting and be transferred to solidified on the filter paper.Altogether the callus of cultivating in the dark in 28 ℃ in the presence of selective agent in containing 2,4 weeks of cultivation on the substratum of 4-D.During the section, form mushroom resistant calli island at this moment.To regeneration culture medium and behind incubation under the light, release of embryo generation potentiality and seedling are in 4-5 week growth subsequently in this material transfer.Seedling is downcut from callus and, wherein seedling is transferred to soil from described substratum containing incubation 2-3 week on the substratum of plant hormone.The hardened seedling is cultivated in the greenhouse under high humidity and short day.

A construct produces about 35 independent T0 rice transformant.With former generation transformant be transferred to the greenhouse from incubator for tissue culture.Behind the copy number of quantitative PCR analysis with checking T-DNA inset, the single copy transgenic plant that only keep performance selective agent tolerance are used to gather in the crops the T1 seed.Seed is gathered in the crops after transplanting subsequently the 3-5 month.Present method produces term single gene seat transformant (Aldemita and Hodges1996, Chan etc. 1993, Hiei etc. 1994) to surpass 50% ratio.

Corn transforms

The conversion of corn is carried out according to the modification method to (1996.Nature Biotech 14745-50) described methods such as Ishida.Conversion in corn be that genotype relies on and only the special genes type can operate and be used for transforming and regeneration.Inbred lines A188 (University of Minnesota) or be the good source of the donor material that is used to transform with A188 as parent's hybrid, but other genotype also can successfully be used.Mealie from maize plant after pollination about 11 days (DAP) results, this moment, the length of immature embryos was about 1 to 1.2mm.Immature embryos is cultivated altogether with the agrobacterium tumefaciens that contains expression vector and transgenic plant take place to recover by organ.The embryo that downcuts is on the callus inducing medium, cultivate on the corn regeneration culture medium subsequently, and wherein said regeneration culture medium contains selective agent (for example imidazolone, but can use the multiple choices mark).Culture plate is cultivated 2-3 week under illumination at 25 ℃, or grows until seedling.Green seedling is transferred to the maize rooting substratum and cultivates 2-3 week at 25 ℃ from each embryo, until root development.The seedling that to take root migrates in the soil in greenhouse.From the plant of performance selective agent T-DNA inset tolerance and that contain single copy, produce the T1 seed.

Wheat transforms

The conversion of wheat is carried out with the method that (1996) Nature Biotech 14 (6): 745-50 such as Ishida such as Ishida describe.Usually in conversion, use (can obtain) Cultivar Bobwhite from Mexico CIMMYT.Immature embryos is cultivated altogether with the agrobacterium tumefaciens that contains expression vector and transgenic plant take place to recover by organ.With the Agrobacterium incubation after, embryo on the callus inducing medium, external cultivation on regeneration culture medium subsequently, wherein said regeneration culture medium contains selective agent (for example imidazolone, but can use the multiple choices mark).Culture plate is cultivated 2-3 week under illumination at 25 ℃, or grows until seedling.Green seedling is transferred to root media and cultivates 2-3 week at 25 ℃ from each embryo, until root development.The seedling that to take root migrates in the soil in greenhouse.From the plant of performance selective agent T-DNA inset tolerance and that contain single copy, produce the T1 seed.

Soybean transforms

According to Texas A﹠amp; M United States Patent (USP) 5,164, the modification method soybean transformation of method described in 310.Several commercial soybean varieties are feasible for conversion by this method.Cultivar Jack (can be able to obtain from Illinois seed money) is generally used for transforming.Soybean seeds is sterilized so that external sowing.From 7 age in days seedling, downcut hypocotyl, radicle and a slice cotyledon.The cotyledon of further cultivating epicotyl and remainder is to grow the armpit tight knot.These armpit tight knots are downcut and with the agrobacterium tumefaciens incubation that contains expression vector.After cultivating processing altogether, explant is washed and is transferred to the selection substratum.The regenerated seedling is downcut and places on the seedling elongation medium.The seedling that length is no more than 1cm places on the root media until root development.The seedling that to take root migrates in the soil in greenhouse.From the plant tolerance of performance selective agent and that contain single copy T-DNA inset, produce the T1 seed.

Rape/canola oil dish transforms

Cotyledon petiole of use 5-6 age in days seedling and hypocotyl are as being used for the explant of tissue culture and transforming according to (1998, Plant Cell Rep 17:183-188) such as Babic.Commercial Cultivar Westar (Agriculture Canada) is the standard variety that is used to transform, but also can use other kinds.Canola oil colza is done the surface sterilization so that external sowing.From external seedling, downcut and have the cotyledon petiole explant that adheres to cotyledon, and immerse bacterial suspension with the cut ends of (containing expression vector) Agrobacterium by petiole explant and inoculate.Explant subsequently on the MSBAP-3 substratum that contains 3mg/l BAP, 3% sucrose, 0.7% plant agar (Phytagar) at 23 ℃, illumination in 16 hours was cultivated 2 days down.After cultivating 2 altogether with Agrobacterium, petiole explant is transferred on the MSBAP-3 substratum of 3mg/l BAP, cefotaxime, Pyocianil or the Ticarcillin/Clavulanate Acid (300mg/l) that contain and continues 7, and cultivate containing on the MSBAP-3 substratum of cefotaxime, Pyocianil or Ticarcillin/Clavulanate Acid and selective agent subsequently, regenerate until seedling.When seedling has 5-10mm length, seedling is downcut and is transferred to seedling elongation medium (MSBAP-0.5 that contains 0.5mg/lBAP).The seedling of the about 2cm of length is transferred to the root media (MS0) that is used for root induction.The seedling that to take root migrates in the soil in greenhouse.Produce the T1 seed the plant that singly copies the T-DNA inset from showing the selective agent tolerance and containing.

Clover transforms

The reproducibility clone of clover uses the method for (McKersie etc., 1999 Plant Physiol 119:839-847) to be transformed.Regeneration of clover and conversion are that genotype is dependent and thereby need aftergrowth.The method that obtains the reproducibility plant has been described.For example, these reproducibility plants any other commercial alfalfa variety that can be selected from Cultivar Rangelander (Agriculture Canada) or describe as Brown DCW and AAtanassov (1985.Plant Cell Tissue Culture 4:111-112).Alternatively, RA3 kind (University of Wisconsin) has been selected for (Walker etc., 1978Am J Bot 65:654-659) in the tissue culture.Petiole explant and the agrobacterium tumefaciens C58C1 pMP90 (McKersie etc., 1999 Plant Physiol 119:839-847) or the overnight culture of LBA4404 that contain expression vector are cultivated altogether.Explant is containing 288mg/L Pro, 53mg/L Thioproline, 4.35g/L K in the dark ₂SO ₄With cultivated altogether 3 days on the SH inducing culture of 100 μ m Syringylethanones. explant half concentrate in the Murashige-Skoog substratum (Murashige and Skoog, 1962) washing and plating contain not containing Syringylethanone suitable selective agent and suitable microbiotic with the identical SH inducing culture of restraining the Agrobacterium growth on.After several weeks, somatic embryo is transferred to do not contain growth regulator, do not contain microbiotic and the BOi2Y that contains 50g/L sucrose grows in the substratum.Somatic embryo concentrates on the Murashige-Skoog substratum half subsequently to be sprouted.The sprigging that to take root is cultivated to flowerpot and in the greenhouse.Produce the T1 seed the plant that singly copies the T-DNA inset from showing the selective agent tolerance and containing.

Cotton transforms

According to US 5,159, the agrobacterium tumefaciens converting cotton of the method described in 135.Cotton seeds places 3% chlorine bleach liquor to carry out surface sterilization in 20 minutes and uses the distilled water wash that contains 500 μ g/ml cefotaximes.Seed is transferred in the SH substratum with 50 μ g/ml F-1991s then and sprouted.Take out the hypocotyl of 4 to 6 age in days seedling, be cut into the 0.5cm fritter and place on 0.8% agar.(every ml about 10 for agrobacterium suspension ⁸Individual cell is from the overnight culture dilution that transforms with goal gene and suitable selective marker) be used to inoculate the hypocotyl explant.After following 3 days of room temperature and the illumination, tissue is transferred in the solid medium (1.6g/l Gelrite), this substratum has Murashige and Skoog salt and B5 VITAMIN (Gamborg et al., Exp.Cell Res.50:151-158 (1968)), 0.1mg/l 2,4-D, 0.1mg/l 6-furfuryl group aminopurine and 750 μ g/ml MgCl ₂, with 50 to 100 μ g/ml cefotaximes and 400-500 μ g/ml carbenicillin to kill residual bacterium.Separate each clone (cultivation of going down to posterity in per 4 to 6 weeks) after 2 to 3 months and further on the selection substratum, cultivate and be used for tissue amplification (30 ℃, the 16hr photoperiod).Organizing of transforming further cultivated 2 to 3 months subsequently to obtain somatic embryo on non-selection substratum.The embryo of the appearance health that 4mm at least is long is transferred to the pipe with the SH substratum that places tiny vermiculite, replenishes the 0.1mg/l indolylacetic acid, 6 furfuryl group aminopurine and gibberic acid.Embryo was cultivated with 16 hour photoperiod under 30 ℃, and the plantlet in 2 to 3 leaf stages transferred to have in vermiculite and the nutraceutical flowerpot.Plant becomes hale and hearty and transfers to the greenhouse subsequently and is used for further cultivation.

Embodiment 6: the phenotype appraisal procedure

6.1 assessment is provided with

Produce about 35 T0 rice transformant independently.With former generation transformant transfer to from tissue culture room and be used for growth and results T1 seed the greenhouse.Keep 6 incidents, wherein the T1 offspring separates with 3: 1 for genetically modified existence/disappearance.For each these incident, express to select about 10 to contain genetically modified T1 seedling (heterozygote and homozygote) and about 10 and lack this genetically modified T1 seedling (inefficacy zygote) by the monitoring witness marking.Transgenic plant and corresponding inefficacy zygote growth side by side on position at random.Greenhouse experiment is short day (illumination in 12 hours), in the illumination 28 ℃, and 22 ℃ and 70% relative humidity in the dark.

4 T1 incidents are further assessed according to the appraisal procedure identical with T1 generation in generation at T2, but each incident has more a plurality of bodies.From the sowing stage to the stage of maturity, plant by the digital imagery case several times.At each time point, every strain plant is taken digital picture (2048x1536 pixel, 1,600 ten thousand number of colors) from least 6 different angles.

The arid screening

In flowerpot soil, cultivate plant under normal operation, up to entering heading stage from six incidents (T2 seed).Then it is transferred to " drying " zone, stop to irrigate.In the flowerpot of selecting at random, insert the humidity detection instrument, with monitoring soil water content (SWC).When SWC is lower than certain threshold value, continue moisturizing from the trend plant, up to reaching normal level once more.Then plant is transferred under the normal condition once more again.Remaining cultivation (plant maturation, seed results) is identical with the plant of not cultivating under the abiotic stress condition.Growth of cultivating under the detail record normal condition and output parameter.

The screening of nitrogen service efficiency

From the rice plant of T2 seed under the normal condition except nutrient solution, grow with potted plant soil in.Flowerpot is ripe with the pouring of specific nutrition solution from being transplanted to, and this solution contains nitrogen (N) content of minimizing, hangs down 7 to 8 times usually.The remainder of cultivating (plant maturation, seed results) with not under the abiotic stress condition growing plants identical.As record growth and output parameter to growth detail under the normal condition.

The salt stress screening

Culturing plants on the matrix of making by coconut fiber and argex (3 to 1 ratios).Sprigging first two weeks to the greenhouse is being used normal nutritive medium.After first two weeks, in nutritive medium, add the salt (NaCl) of 25mM, till the results plant.Measure the relevant parameter of seed then.

6.2 statistical analysis: F-check

With the statistical models of double factor ANOVA (variance analysis) as the net assessment of plant phenotype feature.All parameters with all plant measurements of all incidents of gene transformation of the present invention are carried out the F-check.Carry out F-check with check gene in all transformation events effect and verify the general effect of this gene, be also referred to as the overall potency of gene.The significance threshold value of the true overall potency of gene of F check is made as 5% probability level.Significantly the F-test value points to the potency of gene, and expression not merely is that existence or its position of this gene causes phenotypic difference.

Because carried out two experiments, carried out combined analysis with the eclipsed incident.This for check two experimentally the consistence of effect be useful, and if situation be such, accumulation from the evidence of two experiments so that in conclusion, improve degree of confidence.Used method is a method with mixed model, and it has considered the multilevel hierarchy (i.e. experiment-incident-segregant) of data.Obtain the P-value by comparing likelihood ratio test with the distribution of card side.

6.3 the parameter of measuring

The parameter measurement that biomass is relevant

From the sowing stage to the stage of maturity, plant by the digital imagery case several times.At each time point, every strain plant is taken digital picture (2048x1536 pixel, 1,600 ten thousand number of colors) from least 6 different angles.By the sum of all pixels that is different from background on the digital picture from the ground plant part being counted to determine plant shoot divides area (perhaps leaf biomass).This value is converted into the physical surface value to the image averaging of taking from different perspectives at identical time point and by correction, and it is represented with a square mm.Experiment shows that the over-ground part plant area of measuring by this way is relevant with the biomass of ground plant part.The over-ground part area is the area that plant is measured when reaching the time point of its maximum leaf biomass.Early stage vigor is plant (seedling) over-ground part area of sprouting three weeks of back.The increase of root biomass is expressed as the increase (be measured as in plant life observed maximum biomass) of total root biomass; Perhaps be expressed as root/branch exponential and increase (being measured as the ratio between interim quality of the active growth of root and branch and branch quality).

By the sum of all pixels counting that is different from background from the ground plant part is determined early stage vigor.This value is converted into the physical surface value to the image averaging of taking from different perspectives at identical time point and by correction, represents with a square mm.Result described below sprouts the result of back three all plants.

The parameter measurement that seed is relevant

With sophisticated total panicle results, counting, bar code label use in pack, then 37 ℃ of dryings 3 days down in baking oven.Then with the panicle threshing and collect and count all seeds.Separate full shell and ghost with air-blast device.Abandon ghost and count rest parts once more.On analytical balance, full shell is weighed.Determine the full seed number by remaining full outer hull number behind the counting separating step.By measuring total seed production to weighing from all full shells of plant results.By to total seed number from the every strain plant of shell number count measurement of plant results.Full seed number and their gross weight extrapolation thousand seed weight (TKW) from counting.Harvest index among the present invention (HI) is defined as total seed production and over-ground part area (mm ²) between ratio, multiply by factor 10 again ⁶Each paniculiform ratio of always spending number to be defined as seed sum and ripe total panicle number in the present invention.The full rate of seed as defined herein is the ratio (being expressed as %) that the full seed number accounts in seed (or Xiao Hua) sum.

Embodiment 7: the phenotype assessment result of transgenic plant

Be given in the assessment result of expressing the transgenosis rice plant of HpaG nucleic acid under the non-stress conditions below.Observe the early stage vigor of over-ground part biomass (AreaMax), the vigor that sprouts (emergence vigour)), total seed production, full seed number, full rate, each paniculiform increase (seeing Table C) of spending number, harvest index and thousand seed weight.

Table C: under non-stress conditions, measure the result that output improves

Parameter	Total increase (%)	The p-value of F-check
			??AreaMax	??13	??0.0000
Early stage vigor	??25	??0.0041
			The seed gross weight	??30	??0.0000
The full seed number	??26	??0.0000
			Full rate	??9	??0.0000
Each paniculiform number of spending	??12	??0.0371
			Harvest index	??18	??0.0000
Thousand seed weight	??4	??0.0000

Be given in the assessment result of expressing the transgenosis rice plant of HpaG nucleic acid under the drought stress condition below.Observe the increase (table D) of seed gross weight, full seed number, full rate, harvest index and thousand seed weight.

Table D: output improves measuring result under the drought stress condition

Parameter	Total increase (%)	The p-value of F-check
			The seed gross weight	??40	??0.0000
The full seed number	??37	??0.0000
			Full rate	??30	??0.0000
Harvest index	??37	??0.0000
			Thousand seed weight	??3	??0.0001

Embodiment 8: the Sequence Identification relevant with SEQ ID NO:30 with SEQ ID NO:29

Use the database sequence research tool, as basic local comparison instrument (BLAST) (Altschul etal. (1990) J.Mol. Biol.215:403-410; With Altschul et al. (1997) Nucleic AcidsRes.25:3389-3402) identify sequence (full-length cDNA, ESTs or genomic) and/or with the SEQID NO:30 relevant protein sequence relevant in those sequences of in Entrez Nucleotide database, keeping with SEQ ID NO:29.This program is used to find local similar zone between the sequence by relatively nucleic acid or peptide sequence and sequence library and by calculating the significance,statistical that mates.SEQ ID NO:29 encoded polypeptides is used for the TBLASTN algorithm, uses default setting and filter to ignore the payment of low-complexity sequence.The output result who analyzes shows by paired comparison, and according to probability score (E-value) arrangement, wherein score has reflected the occurrent probability of specific comparison (the E value is low more, and this hits approximately significantly).Except the E value, also pass through identity percentage ratio to relatively marking.Identity percentage ratio refers to identical Nucleotide (or amino acid) number between the nucleic acid of two comparisons on the length-specific (or polypeptide) sequence.In some cases, can adjust default parameters to modify the severity of search.

Table E provides relevant nucleic acid and the peptide sequence of representing with represented nucleotide sequence of SEQ ID NO:29 and SEQ ID NO:30 of peptide sequence to tabulate.

Title	The source is biological	NCBI polypeptide searching number	??NA??SEQ??ID??NO	?AA?SEQ?ID?NO
					??Synecho_PCC6??803SNF2	Cytoalgae PCC 6803 BA000022	??NP_442847.??1	??29	?30
??Anava_SNF2	?Anaebena?variabilis?ATCC?29413	??YP_323780.??1	??31	?32
					??Archaeon??RC-I_SNF2	The product methane archaeon RC-I_SNF2 of Pei Yanging not	??CAJ35100.??1	??33	?34
??Bacce_ATCC10??987_SNF2	Bacillus cereus (Bacillus cereus) ATCC 10987	??AAS44264.??1	??35	?36
					??Crowa_SNF2	?Crocosphaera?watsonii?WH	??ZP_005166	??37	?38

	??8501?ctg336	??13.1
					??Glovi_SNF2	Purple Bacillus adhaerens (Gloeobacter violaceus) PCC 7421	??NP_925212	??39	??40
??Lyn_sp_SNF2	Sheath silk algae (Lyngbya sp.) PCC 8106	??ZP_016223??33.1	??41	??42
					??Metac_C2A_SN??F2	Bite acetate sarcina methanica (Methanosarcina acetivorans) C2A	??NP_615162.??1	??43	??44
??Methu_JF-1_S??NF2	??Methanospirillum?hungatei??JF-1	??ABD41401.??1	??45	??46
					??Metma_Go1_S??NF2	Ma Shi sarcina methanica (Methanosarcina mazei) Goe1	??NP_633503.??1	??47	??48
??Mycbo_SNF2	Mycobacterium bovis (Mycobacterium bovis) BCG Pasteur 1173P2	??CAL72108.??1	??49	??50
					??Myctu_SNF2	Mycobacterium tuberculosis (Mycobacterium tuberculosis) H37Rv	??BX842578.1	??51	??52
??Myxxa_DK_SN??F2	Yellow myxococcus (Myxococcus xanthus) DK 1622	??YP_635387.??1	??53	??54
					??Nocfa_IFM??10152_SNF2	Nocardia farcinica (Nocardia farcinica) IFM 10152	??BAD55876.??1	??55	??56
??Nodsp_SNF2	Produce foam joint ball cyanobacteria (Nodularia spumigena)	??ZP_016291??92.1	??57	??58
					??Nos_sp_PCC71??20_SNF2	Beads algae PCC7120	??BAB78256.??1	??59	??60
??Nos_sp_PCC71??20_SNF2?II	Beads algae PCC 7120	??ZP_001061??50.1	??61	??62
					??Nospu_PCC??73102_SNF2	Point shape beads algae (Nostoc punctiforme) PCC 73102	??NP_488438	??63	??64
??Pelph_BU-1_SN??F2	??Pelodictyon??phaeoclathratiforme?BU-1	??ZP_005894??05.1	??65	??66
					??Proma_CCMP1	??Prochlorococcus?marinus?str.	??NP_874441.	??67	??68

??375_SNF2	??CCMP1375	??1
					??Proma_MIT??9211_SNF2	??Prochlorococcus?marinus?str.??MIT?9211	??ZP_010062??55.1	??69	??70
??Proma_MIT??9303_SNF2	??Prochlorococcus?marinus?str.??MIT?9303	??YP_001018??833.1	??71	??72
					??Proma_MIT931??3_SNF2	??Prochlorococcus?marinus?str.??MIT?9313	??NP_895982.??1	??73	??74
??Rho_sp_RHA1_??SNF2	Rhodococcus (Rhodococcus sp.) RHA1	??ABG93371.??1	??75	??76
					??Saltr_CNB-440??_SNF2	??Salinispora?tropica?CNB-440	??ZP_014313??10	??77	??78
??Symth_IAM148??63_SNF2	??Symbiobacterium??thermophilum?IAM?14863	??BAD39642	??79	??80
					??Syn_sp_WH??5701_SNF2	Synechococcus (Synechococcus sp.) WH 5701	??ZP_010835??91.1	??81	??82
??Syn_sp_BL107_??SNF2	Synechococcus BL107	??ZP_014692??19.1	??83	??84
					??Syn_sp_CC931??1_SNF2	Synechococcus CC9311	??YP_731958.??1	??85	??86
??Syn_sp_CC960??5_SNF2	Synechococcus CC9605	??YP_382805.??1	??87	??88
					??Syn_sp_CC990??2_SNF2	Synechococcus CC9902	??YP_378176.??1	??89	??90
??Syn_sp_RS9916??_SNF2	Synechococcus RS9916	??ZP_014713??62	??91	??92
					??Syn_sp_WH??7805_SNF2	Synechococcus WH	7805	??ZP_011250??39.1	??93	??94
??Syn_sp_WH??8102_SNF2	Synechococcus WH	8102	??NP_898451.??1	??95							??96
					??Synel_PCC6301??_SNF2	Elongated synechococcus (Synechococcus elongatus) PCC 6301	??YP_171376	??97	??98

??Synel_PCC7942??SNF2	Elongated synechococcus PCC 7942	??YP_399891.??1	??99	??100
					??Theel_BP-1_SN??F2	??Thermosynechococcus??elongatus?BP-1	??NP_682403.??1	??101	??102

The supplementary table S1C that Flaus et al. (2006) provides can be seen in the extra source that can be used for implementing SWI2/SNF2 polypeptide of the present invention.The author has been scanned 24 complete archeobacterias and 269 bacterial genomes, and has identified 149 SWI2/SNF2 of SSO1653 subfamily type.

The comparison of embodiment 9:SWI2/SNF2 peptide sequence

Clustal algorithm (1.83) by gradual comparison, (Thompson et al. (1997) Nucleic Acids Res 25:4876-4882 Uses Defaults; Chenna et al. (2003) .Nucleic AcidsRes 31:3497-3500) carried out the comparison of peptide sequence.Result among Fig. 8 shows that the SWI2/SNF2 polypeptide has sequence conservation basically in motif I, Ia, II, III, IV, V, Va and VI (they are added frame), and described motif is expressed as follows:

(i) the motif I LADDMGLGK (T/S) that represents as SEQ ID NO:103 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity by the sequence that increases progressively preferred sequence and motif I;

(ii) (V/I/L) (V/I/L) XNW of (A/C) P (T/M/V) S (V/I/L) of the motif IaL (L/V/I) that represents as SEQ ID NO:104 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity by the sequence that increases progressively preferred sequence and motif Ia;

(iii) (V/I/L) KN of the motif II DEAQ (N/A/H) that represents as SEQ ID NO:105 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity by the sequence that increases progressively preferred sequence and motif II;

(iv) motif III A (L/M) TGTPXEN that represents as SEQ ID NO:106 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity by the sequence that increases progressively preferred sequence and motif III;

(v) motif IV (L/I) XF (T/S) Q (F/Y) that represents as SEQ ID NO:107 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity by the sequence that increases progressively preferred sequence and motif IV;

(vi) (N/T) LTXA (N/S/T) HV of motif VS (L/V) KAGG (V/T/L) G (L/I) that represents as SEQ ID NO:108 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity by the sequence that increases progressively preferred sequence and motif V;

(the vii) motif Va DRWWNPAVE that represents as SEQ ID NO:109 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity by the sequence that increases progressively preferred sequence and motif Va; With

(viii) (F/Y) R (I/L) GQ of motif VIQA (T/S) DR (A/T/V) that represents as SEQ ID NO:110, perhaps have at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity by the sequence that increases progressively preferred sequence and motif VI

Wherein the X among motif Ia, motif III, motif IV and the motif V is an arbitrary amino acid.

These 8 motifs are included in the ATP enzymatic structure territory.ATP enzymatic structure territory is included between terminal last amino acid of C-of first amino-acid residue of motif 1 and SWI2/SNF2 polypeptide (from the N-end to the C-end).Starting and end among Fig. 8 of ATP enzymatic structure territory marks, and the black patch sign of the polypeptide top of comparison is used in ATP enzymatic structure territory self.An example in ATP enzymatic structure territory is the ATP enzymatic structure territory of SEQ ID NO:30, is represented by SEQ ID NO:111.

The sequence mark in 149 SWI2/SNF2SSO1653 subfamily members' ATP enzymatic structure territory is at Flaus et al., provides in (2006) and shows in Fig. 6.Sequence mark is the diagram of amino acid or the comparison of nucleic acid multisequencing.Each mark is made up of several class symbols, each position one class symbol in the sequence.The total height of group is pointed out sequence conservation in this position, and the height of this group internal symbol is pointed out the relative frequency of each amino acid on this position or nucleic acid.Usually, sequence mark provides for example more detailed and more accurate description of ratio consensus sequence of binding site.Provide the algorithm (WebLogo) of this type of mark can be at University of California, the server of Berkeley obtains.Shown in SEQ ID NO:111 and the ATP enzymatic structure territory that is included among the SEQ ID NO:30 be according to the sequence mark as shown in Fig. 6.

From the unrooted radiation of the SWI2/SNF2 polypeptide of many SWI2/SNF2 subfamilies (comprising SSO1653) in abutting connection with tree by Flaus et al., (2006) make up, as shown in Figure 7.Polypeptide shown in SEQ ID NO:30 and all archeobacterias and bacterium (totally being called microorganism) SWI2/SNF2 polypeptide is included in SSO1653 bunch in (the figure centre circle is lived).

Embodiment 10: calculate the overall identity per-cent between the peptide sequence can be used for implementing the inventive method

Use one of this area available method MatGAT (Matrix Global Alignment Tool) software (BMC Bioinformatics.2003 4:29.MatGAT:an application thatgenerates similarity/identity matrices using protein or DNA sequences.Campanella JJ, Bitincka L, Smalley J; The software of Ledion Bitincka trustship) determine to can be used for to implement overall similarity and identity per-cent between the full-length polypeptide sequence of the inventive method.MatGAT software is that DNA or protein sequence have produced similarity/identity matrix and do not need comparison in advance to data.This program uses Myers and Miller overall comparison algorithm to carry out a series of paired comparisons (point penalty 12 is opened in the room, and point penalty 2 is extended in the room), uses for example Blosum 62 (for polypeptide) calculating similarity and identity, then the result is placed distance matrix.Sequence similarity shows in marginal Lower Half and sequence identity shows in the marginal first half in diagonal angle.

The parameter that is used for comparison is:

Rating matrix: Blosum62

First room: 12

Extend the room: 2

Shown the software analysis result (exclusive segment peptide sequence) of overall similarity and identity on the full-length polypeptide sequence among the F at table.Identity per-cent is providing above the diagonal lines and similarity per-cent provides below diagonal lines.

Compare with SEQ ID NO:30, can be used for implementing identity per-cent between the total length SWI2/SNF2 peptide sequence of SSO1653 subfamily of the inventive method and be between 33 to 52% the amino acid identity (table F).

Compare with the ATP enzymatic structure territory shown in SEQ ID NO:111 in being contained in SEQ ID NO:30, the identity per-cent that can be used for implementing between the ATP enzymatic structure territory of SWI2/SNF2 peptide sequence of SSO1653 subfamily of the inventive method is 45 to 70% amino acid identity (table F1).

Embodiment 11: be included in the evaluation of the structural domain of the peptide sequence that is used for implementing the inventive method

The integrated resource in protein families, structural domain and site (The Integrated Resource ofProtein Families, Domains and Sites) (InterPro) database is based on the integrated interface of conventional sign database of the search of text and sequence.The InterPro database has made up these databases, and it uses diverse ways and uses in various degree biological information to obtain protein labeling for the protein of abundant sign.The cooperation database comprises SWISS-PROT, PROSITE, TrEMBL, PRINTS, ProDom and Pfam, Smart and TIGRFAMs.Interpro is in European information biology institute (the European Bioinformatics Institute in the UnitedKingdom) trustship of Britain.

The correlated results of the InterPro scanning of the peptide sequence shown in SEQ ID NO:30 provides in table G.SWI2/SNF2 polypeptide (or rebuilding enzyme) has sequence similarity with helicase (especially SF2 helicase), and described helicase is to use the isolating enzyme of ATP hydrolyzation catalysis DNA chain.Sequence similarity is confined to the ATP enzymatic structure territory of two types of enzymes.

Table G: the InterPro scanning result of the peptide sequence shown in SEQ ID NO:2 (main searching number)

The InterPro searching number	InterPro describes	The original date storehouse	Initial retrieving number	The retrieval name
					??IPR000330	SNF2 is correlated with	??Pfam	??PF00176	??SNF2N
??IPR001650	Helicase, the C-end	??Pfam	??PF00271	??Helicase_C
							??SMART	??SM00490	??HELICc
		??Profile	??PS51194	??Helicase_CTER
					??IPR014001	DEAD-sample helicase, the N-end	??SMART	??SM00487	??DEXDc
??IPR014021	Helicase	??PROFILE	??PS51192	??Helicase_ATP_BIND_1

Superfamily a and 2TP combination

Embodiment 12: the clone of the nucleotide sequence shown in SEQ ID NO:29

Unless otherwise noted, according to (Sambrook (2001) Molecular Cloning:alaboratory manual, 3rd Edition Cold Spring Harbor Laboratory Press, CSH, New York) or Ausubel et al. (1994), Current Protocols in MolecularBiology, the volume 1 of Current Protocols and the standard scheme described in the volume 2 carry out recombinant DNA technology.The standard material of plant molecular work and method are described among the Plant Molecular Biology Labfax (1993) of the R.D.D.Croy that BIOS Scientific PublicationsLtd (UK) and Blackwell Scientific Publications (UK) publish.

Use cytoalgae PCC6803 genomic dna to pass through pcr amplification cytoalgae PCC6803 SWI2/SNF2 gene as template.Primer prm08774 (SEQ ID NO:113; Justice is arranged: 5 '-ggggacaagtttgtacaaaaaagcaggcttaaacaatggcgactatccacggtaat tgg-3 ') and prm08779 (SEQ ID NO:114; Antisense, complementation: 5 '-ggggaccactttgtacaagaaagctgggttcaatcggacgcttcggctt-3 ') (comprise the AttB site that is used for the Gateway reorganization) and be used for pcr amplification.Under standard conditions, use Hifi Taq archaeal dna polymerase to carry out PCR.Also use the PCR fragment of standard method amplification and purifying expection length (comprising the attB site).Carry out the first step of Gateway program: BP reaction then, between this reaction period, recombinate in PCR fragment and the pDONR201 plasmid generation body to produce " entering the clone " (according to Gateway nomenclature).Plasmid pDONR201 is available from Invitrogen, as

The part of technology.

Embodiment 13: use and carry out expression vector establishment as the represented nucleotide sequence of SEQ ID NO:29

The clone that enters who comprises SEQ ID NO:29 is used for the LR reaction with the purpose clone who is used for the rice conversion subsequently.This carrier contains as functional element on the T-DNA border: plant selectable marker; The selection markers expression cassette; Be used for expection and be cloned in the purpose nucleotide sequence that enters the clone Gateway box of recombinating in the LR body takes place.Be used for expanding in early days the upstream that rice β-expansion protein promoter (SEQ ID NO:112) that tissue expresses is positioned at this Gateway box.

After the LR reconstitution steps, the expression vector pExp::SWI2/SNF2 (Fig. 8) with gained is transformed among the agrobacterium strains LBA4044 according to method well known in the art.

Embodiment 14: Plant Transformation

See top embodiment 5 for the rice conversion.

Embodiment 15: the phenotype appraisal procedure

15.1 assessment is provided with

About 35 T0 rice transformant have independently been produced.With former generation transformant transfer to the greenhouse from tissue culture room and be used for growth and results T1 seed.Kept 6 incidents, wherein the T1 offspring separates with 3: 1 for genetically modified existence/disappearance.For these incidents each, express selection by the monitoring visual indicia and contain these genetically modified about 10 T1 seedling (heterozygote and homozygote) and lack genetically modified about 10 T1 seedling (inefficacy zygote).Transgenic plant and corresponding inefficacy zygote growth side by side on position at random.Greenhouse experiment is short day (illumination in 12 hours), in the illumination 28 ℃, and 22 ℃ and 70% relative humidity in the dark.

5 T1 incidents T2 in generation according to the identical appraisal procedure assessment of T1 generation, but each incident has more a plurality of bodies.From the sowing stage to the stage of maturity, plant by the digital imagery case several times.At each time point, every strain plant is taken digital picture (2048x1536 pixel, 1,600 ten thousand number of colors) from least 6 different angles.

The arid screening

In flowerpot soil, cultivate plant under normal operation, up to entering heading stage from 5 incidents (T2 seed).Then it is transferred to " drying " zone, stop to irrigate.In the flowerpot of selecting at random, insert the humidity detection instrument, with monitoring soil water content (SWC).When SWC is lower than certain threshold value, continue moisturizing from the trend plant, up to reaching normal level once more.Then plant is transferred under the normal condition once more again.Remaining cultivation (plant maturation, seed results) is identical with the plant of not cultivating under the abiotic stress condition.Growth of cultivating under the detail record normal condition and output parameter.

The salt stress screening

On the matrix of making by coconut fiber and argex (3 to 1 ratios), cultivate rice plant.Sprigging first two weeks to the greenhouse is being used normal nutritive medium.After first two weeks, add the salt (NaCl) of 25mM in nutritive medium, this nutritive medium comprises the component of listing below.

Concentration is 1kg/m ³The NPK nutritional blend, 20-20-20 Peters professional (Scotts, Marysville, OH, USA)

333.33ml/m ³Magnesium inner complex Chelal Mg (BMS, Bornem, Belgium)

21.67g/m ³Iron chelate Libfer (CIBA, Bradford, UK)

·NaCl?1.425kg/m3

Monitor salt concn weekly and add salt in case of necessity.Plant grows under these conditions and fills up to beginning grain.Then they are transferred in the different compartments in greenhouse, irrigate with fresh water their every days there and gather in the crops up to seed.The same record growth with growth under the normal growth condition and output parameter.

Nutrition (nitrogen) the operability screening that reduces

Except nutritive medium, rice plant is grown in the flowerpot soil under normal operation.Specific nutrition liquid with the nitrogen that contains reduction (N) content is irrigated flowerpot from being transplanted to maturation, is less than usually 7 to 8 times.Remaining cultivation (plant maturation, seed results) is identical with the plant under not growing in biological stress conditions.The same record growth with growth under the normal growth condition and output parameter.

15.2 statistical analysis: F-check

With the statistical models of double factor ANOVA (variance analysis) as the net assessment of plant phenotype feature.All parameters with all plant measurements of all incidents of gene transformation of the present invention are carried out the F-check.Carry out F-check with check gene in all transformation events effect and verify the general effect of this gene, be also referred to as the overall potency of gene.The significance threshold value of the true overall potency of gene of F check is made as 5% probability level.Significantly the F-test value points to the potency of gene, and expression not merely is that existence or its position of this gene caused phenotypic difference.

15.3 the parameter of measuring

The parameter measurement that biomass is relevant

From the sowing stage to the stage of maturity, plant passes the digital imagery case several times.At each time point, every strain plant is taken digital picture (2048x1536 pixel, 1,600 ten thousand number of colors) from least 6 different angles.By the sum of all pixels counting that is different from background on the digital picture from the ground plant part is determined that plant shoot divides area (perhaps leaf biomass).This value is converted into the physical surface value to the image averaging of taking from different perspectives at identical time point and by correction, and it is represented with a square mm.Experiment shows that the over-ground part plant area of measuring by this way is relevant with the biomass of ground plant part.The over-ground part area is the area that plant is measured when reaching the time point of its maximum leaf biomass.Early stage vigor is plant (seedling) over-ground part area of sprouting three weeks of back.

In order to measure the relevant parameter of root, plant-growth is observed root with permission in having the specially designed flowerpot of clear bottom.During plant-growth, pass through the image of flowerpot bottom with the digital camera record.The increase of root biomass is expressed as the increase (be measured as in plant life observed maximum biomass) of total root biomass; Perhaps be expressed as root/branch exponential and increase (being measured as the ratio between interim quality of the active growth of root and branch and branch quality).In addition, calculate the observed maximum biomass that is higher than the root (thick root) of a certain thickness threshold value during plant life, and the maximum biomass that is lower than the root (radicula) of a certain thickness threshold value.

The parameter measurement that seed is relevant

With sophisticated total panicle results, counting, bar code label use in pack, then 37 ℃ of dryings 3 days down in baking oven.Then with the panicle threshing and collect and count all seeds.Separate full shell and ghost with air-blast device.Abandon ghost and count rest parts once more.On analytical balance, full shell is weighed.Determine the full seed number by remaining full shell behind the counting separating step.By to total seed weight from the every strain plant of all full shell weighing measurements of strain plant results.By to total seed number from the every strain plant of shell number count measurement of plant results.Full seed number and their gross weight extrapolation thousand seed weight (TKW) from counting.Harvest index among the present invention (HI) is defined as total seed weight and the over-ground part area (mm of every strain plant ²) between ratio, multiply by factor 10 again ⁶Each paniculiform ratio of always spending number to be defined as seed sum and ripe total panicle number in the present invention.As the full rate of the seed that defines among the present invention is the ratio (being expressed as %) that the full seed number accounts in seed sum (or Xiao Hua).

Embodiment 16: the phenotype assessment result of the transgenosis rice plant of Sheng Chang expression SWI2/SNF2 nucleotide sequence under normal operation

Show in following table H in the assessment result of expressing the transgenosis rice plant of SWI2/SNF2 nucleotide sequence under the normal growth condition.

Compare with corresponding invalid zygote (contrast), total seed weight, seed sum, full seed number and the harvest index of the paniculiform colored number of each of transgenic plant, every strain plant have all increased.

Table H expresses the assessment result of the transgenosis rice plant of SWI2/SNF2 nucleotide sequence under the normal growth condition.

	The average increase % of the incident that T1 behaved oneself best in generation	The average increase % of the incident that T2 behaved oneself best in generation
			Each paniculiform colored number	??11％	??3％
Total seed weight of every strain plant	??13％	??28％
			The seed sum	??14％	??6％
The full seed number	??14％	??25％
			Harvest index	??10％	??25％

Embodiment 17: the phenotype assessment result of the transgenosis rice plant of growing under the drought stress condition

The assessment result of expressing the transgenosis rice plant of SWI2/SNF2 nucleotide sequence under the drought stress growth conditions provides in Table I.

Compare with corresponding invalid zygote (contrast), total seed weight, seed sum, full seed number and the harvest index of the over-ground part area of transgenic plant, total root biomass, each paniculiform colored number, the full rate of seed, every strain plant have all increased.

Table I is expressed the assessment result of the transgenosis rice plant of SWI2/SNF2 nucleotide sequence under the drought stress growth conditions.

	The average increase % of the incident that T2 behaved oneself best in generation
		The over-ground part area	??16％
Total root biomass	??13％
		Thick root biomass	??10％
The radicula biomass	??13％
		Each paniculiform colored number	??7％
The full rate of seed	??28％
		Total seed weight of every strain plant	??57％
The seed sum	??44％
		The full seed number	??54％
Harvest index	??31％

Embodiment 18: the conversion example of corn, clover, cotton, soybean, Semen Brassicae campestris/rape, wheat is seen top embodiment 5.

Sequence table

＜110〉Cropdesign NV

Plant function genome center

＜120〉has the plant and preparation method thereof of enhanced yield correlated character

<130>PF58891

<160>114

＜170〉PatentIn version 3 .3

<210>1

<211>402

<212>DNA

＜213〉carpetweed Xanthomonas campestris

<400>1

atgaattctt?tgaacacaca?gctcggcgcc?aactcgtcct?tctttcaggt?tgaccccggc?????60

cagaacacgc?aatctagtcc?gaaccagggc?aaccagggca?tctcggaaaa?gcaactggac????120

cagctgctga?cccagctcat?catggccctg?cttcagcaga?gcaacaatgc?cgagcagggt????180

cagggtcaag?gccagggtgg?tgactctggc?ggtcagggcg?gcaatccgcg?gcaggccggg????240

cagtccaacg?gctccccctc?gcaatacacc?caggcgctga?tgaatatcgt?cggagacatt????300

ctccaggcgc?agaatggtgg?cggcttcggc?ggcggctttg?gtggtggctt?cggtggcatc????360

ctcgtcacca?gccttgcgag?cgacaccgga?tcgatgcagt?aa???????????????????????402

<210>2

<211>133

<212>PRT

＜213〉carpetweed Xanthomonas campestris

<400>2

Met?Asn?Ser?Leu?Asn?Thr?Gln?Leu?Gly?Ala?Asn?Ser?Ser?Phe?Phe?Gln

1???????????????5???????????????????10??????????????????15

Val?Asp?Pro?Gly?Gln?Asn?Thr?Gln?Ser?Ser?Pro?Asn?Gln?Gly?Asn?Gln

20??????????????????25??????????????????30

Gly?Ile?Ser?Glu?Lys?Gln?Leu?Asp?Gln?Leu?Leu?Thr?Gln?Leu?Ile?Met

35???????????????40???????????????45

Ala?Leu?Leu?Gln?Gln?Ser?Asn?Asn?Ala?Glu?Gln?Gly?Gln?Gly?Gln?Gly

50??????????????????55??????????????????60

Gln?Gly?Gly?Asp?Ser?Gly?Gly?Gln?Gly?Gly?Asn?Pro?Arg?Gln?Ala?Gly

65??????????????????70??????????????????75???????????????80

Gln?Ser?Asn?Gly?Ser?Pro?Ser?Gln?Tyr?Thr?Gln?Ala?Leu?Met?Asn?Ile

85??????????????????90??????????????????95

Val?Gly?Asp?Ile?Leu?Gln?Ala?Gln?Asn?Gly?Gly?Gly?Phe?Gly?Gly?Gly

100?????????????????105?????????????????110

Phe?Gly?Gly?Gly?Phe?Gly?Gly?Ile?Leu?Val?Thr?Ser?Leu?Ala?Ser?Asp

115?????????????????120?????????????????125

Thr?Gly?Ser?Met?Gln

130

<210>3

<211>11

<212>PRT

＜213〉artificial sequence

<220>

＜223〉conservative motif 1

<220>

＜221〉variant

<222>(2)..(2)

＜223 〉/replace=" Glu "/replace=" Asp "

<220>

＜221〉variant

<222>(3)..(3)

＜223 〉/replace=" Glu "

<220>

＜221〉uncertain

<222>(4)..(4)

＜223〉Xaa can be arbitrary naturally occurring amino acid

<220>

＜221〉variant

<222>(5)..(5)

＜223 〉/replace=" Arg "/replace=" Pro "

<220>

＜221〉variant

<222>(7)..(7)

＜223 〉/replace=" Ser "

<220>

＜221〉uncertain

<222>(9)..(9)

＜223〉Xaa can be arbitrary naturally occurring amino acid

<220>

＜221〉variant

<222>(10)..(10)

＜223 〉/replace=" Asp "

<400>3

Gly?Gly?Asn?Xaa?Gln?Gln?Ala?Gly?Xaa?Asn?Gly

1???????????????5???????????????????10

<210>4

<211>14

<212>PRT

＜213〉artificial sequence

<220>

＜223〉conservative motif 2

<220>

＜221〉variant

<222>(1)..(1)

＜223 〉/replace=" Ala "/replace=" Val "

<220>

＜221〉variant

<222>(3)..(3)

＜223 〉/replace=" Gln "/replace=" Ala "

<220>

＜221〉variant

<222>(4)..(4)

＜223 〉/replace=" Leu "/replace=" Tyr "

<220>

＜221〉variant

<222>(7)..(7)

＜223 〉/replace=" Ala "

<220>

＜221〉variant

<222>(10)..(10)

＜223 〉/replace=" Asn "/replace=" Gln "

<220>

＜221〉variant

<222>(13)..(13)

＜223 〉/replace=" Met "

<220>

＜221〉variant

<222>(14)..(14)

＜223 〉/replace=" Asp "/replace=" Gln "

<400>4

Pro?Ser?Pro?Phe?Thr?Gln?Met?Leu?Met?His?Ile?Val?Gly?Glu

1???????????????5???????????????????10

<210>5

<211>2194

<212>DNA

＜213〉rice

<400>5

aatccgaaaa?gtttctgcac?cgttttcacc?ccctaactaa?caatataggg?aacgtgtgct??????60

aaatataaaa?tgagacctta?tatatgtagc?gctgataact?agaactatgc?aagaaaaact?????120

catccaccta?ctttagtggc?aatcgggcta?aataaaaaag?agtcgctaca?ctagtttcgt?????180

tttccttagt?aattaagtgg?gaaaatgaaa?tcattattgc?ttagaatata?cgttcacatc?????240

tctgtcatga?agttaaatta?ttcgaggtag?ccataattgt?catcaaactc?ttcttgaata?????300

aaaaaatctt?tctagctgaa?ctcaatgggt?aaagagagag?atttttttta?aaaaaataga?????360

atgaagatat?tctgaacgta?ttggcaaaga?tttaaacata?taattatata?attttatagt?????420

ttgtgcattc?gtcatatcgc?acatcattaa?ggacatgtct?tactccatcc?caatttttat?????480

ttagtaatta?aagacaattg?acttattttt?attatttatc?ttttttcgat?tagatgcaag?????540

gtacttacgc?acacactttg?tgctcatgtg?catgtgtgag?tgcacctcct?caatacacgt?????600

tcaactagca?acacatctct?aatatcactc?gcctatttaa?tacatttagg?tagcaatatc?????660

tgaattcaag?cactccacca?tcaccagacc?acttttaata?atatctaaaa?tacaaaaaat?????720

aattttacag?aatagcatga?aaagtatgaa?acgaactatt?taggtttttc?acatacaaaa?????780

aaaaaaagaa?ttttgctcgt?gcgcgagcgc?caatctccca?tattgggcac?acaggcaaca?????840

acagagtggc?tgcccacaga?acaacccaca?aaaaacgatg?atctaacgga?ggacagcaag?????900

tccgcaacaa?ccttttaaca?gcaggctttg?cggccaggag?agaggaggag?aggcaaagaa?????960

aaccaagcat?cctccttctc?ccatctataa?attcctcccc?ccttttcccc?tctctatata????1020

ggaggcatcc?aagccaagaa?gagggagagc?accaaggaca?cgcgactagc?agaagccgag????1080

cgaccgcctt?ctcgatccat?atcttccggt?cgagttcttg?gtcgatctct?tccctcctcc????1140

acctcctcct?cacagggtat?gtgcctccct?tcggttgttc?ttggatttat?tgttctaggt????1200

tgtgtagtac?gggcgttgat?gttaggaaag?gggatctgta?tctgtgatga?ttcctgttct????1260

tggatttggg?atagaggggt?tcttgatgtt?gcatgttatc?ggttcggttt?gattagtagt????1320

atggttttca?atcgtctgga?gagctctatg?gaaatgaaat?ggtttaggga?tcggaatctt????1380

gcgattttgt?gagtaccttt?tgtttgaggt?aaaatcagag?caccggtgat?tttgcttggt????1440

gtaataaagt?acggttgttt?ggtcctcgat?tctggtagtg?atgcttctcg?atttgacgaa????1500

gctatccttt?gtttattccc?tattgaacaa?aaataatcca?actttgaaga?cggtcccgtt????1560

gatgagattg?aatgattgat?tcttaagcct?gtccaaaatt?tcgcagctgg?cttgtttaga????1620

tacagtagtc?cccatcacga?aattcatgga?aacagttata?atcctcagga?acaggggatt????1680

ccctgttctt?ccgatttgct?ttagtcccag?aatttttttt?cccaaatatc?ttaaaaagtc????1740

actttctggt?tcagttcaat?gaattgattg?ctacaaataa?tgcttttata?gcgttatcct????1800

agctgtagtt?cagttaatag?gtaatacccc?tatagtttag?tcaggagaag?aacttatccg????1860

atttctgatc?tccattttta?attatatgaa?atgaactgta?gcataagcag?tattcatttg????1920

gattattttt?tttattagct?ctcacccctt?cattattctg?agctgaaagt?ctggcatgaa????1980

ctgtcctcaa?ttttgttttc?aaattcacat?cgattatcta?tgcattatcc?tcttgtatct????2040

acctgtagaa?gtttcttttt?ggttattcct?tgactgcttg?attacagaaa?gaaatttatg????2100

aagctgtaat?cgggatagtt?atactgcttg?ttcttatgat?tcatttcctt?tgtgcagttc????2160

ttggtgtagc?ttgccacttt?caccagcaaa?gttc????????????????????????????????2194

<210>6

<211>1179

<212>DNA

＜213〉rice

<400>6

ttgcagttgt?gaccaagtaa?gctgagcatg?cccttaactt?cacctagaaa?aaagtatact??????60

tggcttaact?gctagtaaga?catttcagaa?ctgagactgg?tgtacgcatt?tcatgcaagc?????120

cattaccact?ttacctgaca?ttttggacag?agattagaaa?tagtttcgta?ctacctgcaa?????180

gttgcaactt?gaaaagtgaa?atttgttcct?tgctaatata?ttggcgtgta?attcttttat?????240

gcgttagcgt?aaaaagttga?aatttgggtc?aagttactgg?tcagattaac?cagtaactgg?????300

ttaaagttga?aagatggtct?tttagtaatg?gagggagtac?tacactatcc?tcagctgatt?????360

taaatcttat?tccgtcggtg?gtgatttcgt?caatctccca?acttagtttt?tcaatatatt?????420

cataggatag?agtgtgcata?tgtgtgttta?tagggatgag?tctacgcgcc?ttatgaacac?????480

ctacttttgt?actgtatttg?tcaatgaaaa?gaaaatctta?ccaatgctgc?gatgctgaca?????540

ccaagaagag?gcgatgaaaa?gtgcaacgga?tatcgtgcca?cgtcggttgc?caagtcagca?????600

cagacccaat?gggcctttcc?tacgtgtctc?ggccacagcc?agtcgtttac?cgcacgttca?????660

catgggcacg?aactcgcgtc?atcttcccac?gcaaaacgac?agatctgccc?tatctggtcc?????720

cacccatcag?tggcccacac?ctcccatgct?gcattatttg?cgactcccat?cccgtcctcc?????780

acgcccaaac?accgcacacg?ggtcgcgata?gccacgaccc?aatcacacaa?cgccacgtca?????840

ccatatgtta?cgggcagcca?tgcgcagaag?atcccgcgac?gtcgctgtcc?cccgtgtcgg?????900

ttacgaaaaa?atatcccacc?acgtgtcgct?ttcacaggac?aatatctcga?aggaaaaaaa?????960

tcgtagcgga?aaatccgagg?cacgagctgc?gattggctgg?gaggcgtcca?gcgtggtggg????1020

gggcccaccc?ccttatcctt?agcccgtggc?gctcctcgct?cctcgggtcc?gtgtataaat????1080

accctccgga?actcactctt?gctggtcacc?aacacgaagc?aaaaggacac?cagaaacata????1140

gtacacttga?gctcactcca?aactcaaaca?ctcacacca???????????????????????????1179

<210>7

<211>402

<212>DNA

＜213〉artificial sequence

<220>

＜223〉anaphylactoid synthetic construct sudden change trigger molecule HpaG_T44C gene

<400>7

atgaattctt?tgaacacaca?gctcggcgcc?aactcgtcct?tctttcaggt?tgaccccggc?????60

cagaacacgc?aatctagtcc?gaaccagggc?aaccagggca?tctcggaaaa?gcaactggac????120

cagctgctgt?gccagctcat?catggccctg?cttcagcaga?gcaacaatgc?cgagcagggt????180

cagggtcaag?gccagggtgg?tgactctggc?ggtcagggcg?gcaatccgcg?gcaggccggg????240

cagtccaacg?gctccccctc?gcaatacacc?caggcgctga?tgaatatcgt?cggagacatt????300

ctccaggcgc?agaatggtgg?cggcttcggc?ggcggctttg?gtggtggctt?cggtggcatc????360

ctcgtcacca?gccttgcgag?cgacaccgga?tcgatgcagt?aa???????????????????????402

<210>8

<211>133

<212>PRT

＜213〉artificial sequence

<220>

＜223〉anaphylactoid sudden change trigger molecule HpaG_T44C

<400>8

Met?Asn?Ser?Leu?Asn?Thr?Gln?Leu?Gly?Ala?Asn?Ser?Ser?Phe?Phe?Gln

1???????????????5???????????????????10??????????????????15

Val?Asp?Pro?Gly?Gln?Asn?Thr?Gln?Ser?Ser?Pro?Asn?Gln?Gly?Asn?Gln

20??????????????????25??????????????????30

Gly?Ile?Ser?Glu?Lys?Gln?Leu?Asp?Gln?Leu?Leu?Cys?Gln?Leu?Ile?Met

35??????????????????40??????????????????45

Ala?Leu?Leu?Gln?Gln?Ser?Asn?Asn?Ala?Glu?Gln?Gly?Gln?Gly?Gln?Gly

50??????????????????55??????????????????60

Gln?Gly?Gly?Asp?Ser?Gly?Gly?Gln?Gly?Gly?Asn?Pro?Arg?Gln?Ala?Gly

65??????????????????70??????????????????75??????????????????80

Gln?Ser?Asn?Gly?Ser?Pro?Ser?Gln?Tyr?Thr?Gln?Ala?Leu?Met?Asn?Ile

85??????????????????90??????????????????95

Val?Gly?Asp?Ile?Leu?Gln?Ala?Gln?Asn?Gly?Gly?Gly?Phe?Gly?Gly?Gly

100?????????????????105?????????????????110

Phe?Gly?Gly?Gly?Phe?Gly?Gly?Ile?Leu?Val?Thr?Ser?Leu?Ala?Ser?Asp

115?????????????????120?????????????????125

Thr?Gly?Ser?Met?Gln

130

<210>9

<211>378

<212>DNA

＜213〉artificial sequence

<220>

＜223〉anaphylactoid synthetic construct sudden change trigger molecule

The HpaG-T gene

<400>9

atgaattctt?tgaacacaca?gctcggcgcc?aactcgtcct?tctttcaggt?tgaccccggc?????60

cagaacacgc?aatctagtcc?gaaccagggc?aaccagggca?tctcggaaaa?gcaactggac????120

cagctgctga?cccagctcat?catggccctg?cttcagcaga?gcaacaatgc?cgagcagggt????180

cagggtcaag?gccagggtgg?tgactctggc?ggtcagggcg?gcaatccgcg?gcaggccggg????240

cagtccaacg?gctccccctc?gcaatacacc?caggcgctga?tgaatatcgt?cggagacggc????300

ttcggcggcg?gctttggtgg?tggcttcggt?ggcatcctcg?tcaccagcct?tgcgagcgac????360

accggatcga?tgcagtaa???????????????????????????????????????????378

<210>10

<211>125

<212>PRT

＜213〉artificial sequence

<220>

＜223〉anaphylactoid sudden change trigger molecule HpaG-T

<400>10

Met?Asn?Ser?Leu?Asn?Thr?Gln?Leu?Gly?Ala?Asn?Ser?Ser?Phe?Phe?Gln

1???????????????5???????????????????10??????????????????15

Val?Asp?Pro?Gly?Gln?Asn?Thr?Gln?Ser?Ser?Pro?Asn?Gln?Gly?Asn?Gln

20??????????????????25??????????????????30

Gly?Ile?Ser?Glu?Lys?Gln?Leu?Asp?Gln?Leu?Leu?Thr?Gln?Leu?Ile?Met

35??????????????????40??????????????????45

Ala?Leu?Leu?Gln?Gln?Ser?Asn?Asn?Ala?Glu?Gln?Gly?Gln?Gly?Gln?Gly

50??????????????????55??????????????????60

Gln?Gly?Gly?Asp?Ser?Gly?Gly?Gln?Gly?Gly?Asn?Pro?Arg?Gln?Ala?Gly

65??????????????????70??????????????????75??????????????????80

Gln?Ser?Asn?Gly?Ser?Pro?Ser?Gln?Tyr?Thr?Gln?Ala?Leu?Met?Asn?Ile

85??????????????????90??????????????????95

Val?Gly?Asp?Gly?Phe?Gly?Gly?Gly?Phe?Gly?Gly?Gly?Phe?Gly?Gly?Ile

100?????????????????105?????????????????110

Leu?Val?Thr?Ser?Leu?Ala?Ser?Asp?Thr?Gly?Ser?Met?Gln

115?????????????????120?????????????????125

<210>11

<211>414

<212>DNA

＜213〉carpetweed Xanthomonas campestris pv.citri

<400>11

ttactgcatc?gatccggtgt?cgctcgcaag?gctggtgccg?aggctggtgc?cgaggccgcc???60

gccgaagcca?ccaccaaagc?cgccgccgaa?gccaccacca?ttctgcgcct?ggagaatgtc??120

tccgacgata?ttcatcagca?tctgggtgta?ttgcgagggg?gagccgttgg?actgaccggc??180

ctgctgccga?ttgccgccct?gaccaccaga?gtcaccaccc?tggccttgac?cctgaccctg??240

ctcggcattg?ttgctctgct?gaagcagggc?catgatgagc?tgggtcagca?gctggtccag??300

ttgcttttcc?gagatgccct?ggttgccctg?gttcgaacca?gattgcgtgt?tctggctggg??360

gtcaacctga?aagaaggacg?agttggcgcc?gagctgtgtg?ttcaaagaat?tcat????????414

<210>12

<211>137

<212>PRT

＜213〉carpetweed Xanthomonas campestris pv.citri

<400>12

Met?Asn?Ser?Leu?Asn?Thr?Gln?Leu?Gly?Ala?Asn?Ser?Ser?Phe?Phe?Gln

1???????????????5???????????????????10??????????????????15

Val?Asp?Pro?Ser?Gln?Asn?Thr?Gln?Ser?Gly?Ser?Asn?Gln?Gly?Asn?Gln

20??????????????????25??????????????????30

Gly?Ile?Ser?Glu?Lys?Gln?Leu?Asp?Gln?Leu?Leu?Thr?Gln?Leu?Ile?Met

35??????????????????40??????????????????45

Ala?Leu?Leu?Gln?Gln?Ser?Asn?Asn?Ala?Glu?Gln?Gly?Gln?Gly?Gln?Gly

50??????????????????55??????????????????60

Gln?Gly?Gly?Asp?Ser?Gly?Gly?Gln?Gly?Gly?Asn?Arg?Gln?Gln?Ala?Gly

65??????????????????70??????????????????75??????????????????80

Gln?Ser?Asn?Gly?Ser?Pro?Ser?Gln?Tyr?Thr?Gln?Met?Leu?Met?Asn?Ile

85??????????????????90??????????????????95

Val?Gly?Asp?Ile?Leu?Gln?Ala?Gln?Asn?Gly?Gly?Gly?Phe?Gly?Gly?Gly

100?????????????????105?????????????????110

Phe?Gly?Gly?Gly?Phe?Gly?Gly?Gly?Leu?Gly?Thr?Ser?Leu?Gly?Thr?Ser

115?????????????????120?????????????????125

Leu?Ala?Ser?Asp?Thr?Gly?Ser?Met?Gln

130?????????????????135

<210>13

<211>366

<212>DNA

＜213〉artificial sequence

<220>

＜223〉anaphylactoid synthetic construct sudden change trigger molecule

The HpaG-N gene

<400>13

atgaattctt?tgaacacaca?gctcggcgcc?aactcgtcct?tctttcaggt?tgaccccggc?????60

cagaacacgc?aatctagtcc?gaaccagggc?aacacccagc?tcatcatggc?cctgcttcag????120

cagagcaaca?atgccgagca?gggtcagggt?caaggccagg?gtggtgactc?tggcggtcag????180

ggcggcaatc?cgcggcaggc?cgggcagtcc?aacggctccc?cctcgcaata?cacccaggcg????240

ctgatgaata?tcgtcggaga?cattctccag?gcgcagaatg?gtggcggctt?cggcggcggc????300

tttggtggtg?gcttcggtgg?catcctcgtc?accagccttg?cgagcgacac?cggatcgatg????360

cagtaa???????????????????????????????????????????????????????366

<210>14

<211>121

<212>PRT

＜213〉artificial sequence

<220>

＜223〉anaphylactoid sudden change trigger molecule HpaG-N

<400>14

Met?Asn?Ser?Leu?Asn?Thr?Gln?Leu?Gly?Ala?Asn?Ser?Ser?Phe?Phe?Gln

1???????????????5???????????????????10??????????????????15

Val?Asp?Pro?Gly?Gln?Asn?Thr?Gln?Ser?Ser?Pro?Asn?Gln?Gly?Asn?Thr

20??????????????????25??????????????????30

Gln?Leu?Ile?Met?Ala?Leu?Leu?Gln?Gln?Ser?Asn?Asn?Ala?Glu?Gln?Gly

35??????????????????40??????????????????45

Gln?Gly?Gln?Gly?Gln?Gly?Gly?Asp?Ser?Gly?Gly?Gln?Gly?Gly?Asn?Pro

50??????????????????55??????????????????60

Arg?Gln?Ala?Gly?Gln?Ser?Asn?Gly?Ser?Pro?Ser?Gln?Tyr?Thr?Gln?Ala

65??????????????????70??????????????????75??????????????????80

Leu?Met?Asn?Ile?Val?Gly?Asp?Ile?Leu?Gln?Ala?Gln?Asn?Gly?Gly?Gly

85??????????????????90??????????????????95

Phe?Gly?Gly?Gly?Phe?Gly?Gly?Gly?Phe?Gly?Gly?Ile?Leu?Val?Thr?Ser

100?????????????????105?????????????????110

Leu?Ala?Ser?Asp?Thr?Gly?Ser?Met?Gln

115?????????????????120

<210>15

<211>366

<212>DNA

＜213〉carpetweed Xanthomonas campestris

<400>15

atgaattctt?tgaacacaca?gctcggcgcc?aactcgtcct?tctttcaggt?tgaccccggc?????60

cagaacacgc?aatctagtcc?gaaccagggc?aaccagggca?tctcggaaaa?gcaactggac????120

cagctgctga?cccagctcat?catggccctg?cttcagcaga?gcaacaatgc?cgagcagggt????180

cagggtcaag?gccagggtgg?tgactctggc?ggtcagggcg?gcaatccgcg?gcaggccggg????240

cagtccaacg?gctccccctc?gcaatacacc?caggcgctga?tgaatatcgt?cggagacatt????300

ctccaggcgc?agaatggctt?tatcctcgtc?accagccttg?cgagcgacac?cggatcgatg????360

cagtaa???????????????????????????????????????????????????????????????366

<210>16

<211>121

<212>PRT

＜213〉carpetweed Xanthomonas campestris

<400>16

Met?Asn?Ser?Leu?Asn?Thr?Gln?Leu?Gly?Ala?Asn?Ser?Ser?Phe?Phe?Gln

1???????????????5???????????????????10??????????????????15

Val?Asp?Pro?Gly?Gln?Asn?Thr?Gln?Ser?Ser?Pro?Asn?Gln?Gly?Asn?Gln

20??????????????????25??????????????????30

Gly?Ile?Ser?Glu?Lys?Gln?Leu?Asp?Gln?Leu?Leu?Thr?Gln?Leu?Ile?Met

35??????????????????40??????????????????45

Ala?Leu?Leu?Gln?Gln?Ser?Asn?Asn?Ala?Glu?Gln?Gly?Gln?Gly?Gln?Gly

50??????????????????55??????????????????60

Gln?Gly?Gly?Asp?Ser?Gly?Gly?Gln?Gly?Gly?Asn?Pro?Arg?Gln?Ala?Gly

65??????????????????70??????????????????75??????????????????80

Gln?Ser?Asn?Gly?Ser?Pro?Ser?Gln?Tyr?Thr?Gln?Ala?Leu?Met?Asn?Ile

85??????????????????90??????????????????95

Val?Gly?Asp?Ile?Leu?Gln?Ala?Gln?Asn?Gly?Phe?Ile?Leu?Val?Thr?Ser

100?????????????????105?????????????????110

Leu?Ala?Ser?Asp?Thr?Gly?Ser?Met?Gln

115?????????????????120

<210>17

<211>402

<212>DNA

<213>Xanthomonas?smithii?subsp.smithii

<400>17

atgaattctt?tgaacacaca?gatcggcgcc?aactcgtcct?tcttgcaggt?cgacccgagc?????60

cagaacacgc?aattcggtcc?gaaccagggc?aatcaaggca?tctcggaaaa?gcagctggac????120

cagctgctga?cccagctcat?catggccctg?cttcagcaga?gcaacaatgc?cgaccagggt????180

cagggtggtg?actctggtgg?tcaaggcggc?aattcgcggc?aggccgggca?gcccaatggt????240

tccccctcgg?catacaccca?gatgctgatg?aatatcgtcg?gagacattct?ccaggcgcag????300

aatggtggtg?gcttcggcgg?cgggttcggc?ggtggctttg?gtggcgggct?cggcaccagc????360

ctcggcagca?gccttgcgag?cgacaccgga?tcgatgcagt?aa???????????????????????402

<210>18

<211>133

<212>PRT

<213>Xanthomonas?smithii?subsp.smithii

<400>18

Met?Asn?Ser?Leu?Asn?Thr?Gln?Ile?Gly?Ala?Asn?Ser?Ser?Phe?Leu?Gln

1???????????????5???????????????????10??????????????????15

Val?Asp?Pro?Ser?Gln?Asn?Thr?Gln?Phe?Gly?Pro?Asn?Gln?Gly?Asn?Gln

20??????????????????25??????????????????30

Gly?Ile?Ser?Glu?Lys?Gln?Leu?Asp?Gln?Leu?Leu?Thr?Gln?Leu?Ile?Met

35??????????????????40??????????????????45

Ala?Leu?Leu?Gln?Gln?Ser?Asn?Asn?Ala?Asp?Gln?Gly?Gln?Gly?Gly?Asp

50??????????????????55??????????????????60

Ser?Gly?Gly?Gln?Gly?Gly?Asn?Ser?Arg?Gln?Ala?Gly?Gln?Pro?Asn?Gly

65??????????????????70??????????????????75??????????????????80

Ser?Pro?Ser?Ala?Tyr?Thr?Gln?Met?Leu?Met?Asn?Ile?Val?Gly?Asp?Ile

85??????????????????90??????????????????95

Leu?Gln?Ala?Gln?Asn?Gly?Gly?Gly?Phe?Gly?Gly?Gly?Phe?Gly?Gly?Gly

100?????????????????105?????????????????110

Phe?Gly?Gly?Gly?Leu?Gly?Thr?Ser?Leu?Gly?Ser?Ser?Leu?Ala?Ser?Asp

115?????????????????120?????????????????125

Thr?Gly?Ser?Met?Gln

130

<210>19

<211>420

<212>DNA

＜213〉the pathogenic mutation of rice Xanthomonas paddy rice

<400>19

atgaactctt?tgaacacaca?attcggcggc?agcacgtcca?accttcaggt?tggcccaagc????60

caggacacaa?cgttcggttc?gaaccagggc?ggcaaccagg?gcatctcgga?aaagcaactg????120

gaccagttgc?tgtgccagct?catctcggcc?ctgcttcagt?cgagcaaaaa?tgctgaggag????180

ggtaagggtc?agggtggcga?taatggcggt?ggccagggcg?gcaattcgca?gcaggccggg????240

cagcagaatg?gcccctcgcc?attcacccag?atgctgatgc?atatcgtcgg?agagattctc????300

caggcgcaga?atggtggtgg?tgctggtggc?ggcggtttcg?gcggcgggtt?cggcggcgac????360

tttagtggcg?acctcggcct?cggcaccaac?ctctcgagcg?acagcgcatc?aatgcagtaa????420

<210>20

<211>139

<212>PRT

＜213〉the pathogenic mutation of rice Xanthomonas paddy rice

<400>20

Met?Asn?Ser?Leu?Asn?Thr?Gln?Phe?Gly?Gly?Ser?Thr?Ser?Asn?Leu?Gln

1???????????????5???????????????????10??????????????????15

Val?Gly?Pro?Ser?Gln?Asp?Thr?Thr?Phe?Gly?Ser?Asn?Gln?Gly?Gly?Asn

20??????????????????25??????????????????30

Gln?Gly?Ile?Ser?Glu?Lys?Gln?Leu?Asp?Gln?Leu?Leu?Cys?Gln?Leu?Ile

35??????????????????40??????????????????45

Ser?Ala?Leu?Leu?Gln?Ser?Ser?Lys?Asn?Ala?Glu?Glu?Gly?Lys?Gly?Gln

50??????????????????55??????????????????60

Gly?Gly?Asp?Asn?Gly?Gly?Gly?Gln?Gly?Gly?Asn?Ser?Gln?Gln?Ala?Gly

65??????????????????70??????????????????75??????????????????80

Gln?Gln?Asn?Gly?Pro?Ser?Pro?Phe?Thr?Gln?Met?Leu?Met?His?Ile?Val

85??????????????????90??????????????????95

Gly?Glu?Ile?Leu?Gln?Ala?Gln?Asn?Gly?Gly?Gly?Ala?Gly?Gly?Gly?Gly

100?????????????????105?????????????????110

Phe?Gly?Gly?Gly?Phe?Gly?Gly?Asp?Phe?Ser?Gly?Asp?Leu?Gly?Leu?Gly

115?????????????????120?????????????????125

Thr?Asn?Leu?Ser?Ser?Asp?Ser?Ala?Ser?Met?Gln

130?????????????????135

<210>21

<211>420

<212>DNA

＜213〉the pathogenic mutation of rice Xanthomonas paddy rice

<400>21

atgaattctt?tgaacacaca?attcggcggc?agcacgtcca?accttcaggt?tggcccaagc?????60

caggacacaa?cgttcggttc?gaaccagggc?ggcaaccagg?gcatctcgga?aaagcaactg????120

gaccagttgc?tgtgccagct?catctcggcc?ctgcttcagt?cgagcaaaaa?tgctgaggag????180

ggtaagggtc?agggtggcga?taatggcggt?ggccagggcg?gcaattcgca?gcaggctggg????240

cagcagaatg?gcccctcgcc?attcacccag?atgctgatgc?atatcgtcgg?agagattctc????300

caggcgcaga?atggtggtgg?tgctggtggc?ggcgggttcg?gcggcgggtt?cggcggtgac????360

tttagtggcg?acctcggcct?cggcaccaac?ctctcgagcg?acagcgcatc?gatgcagtaa????420

<210>22

<211>139

<212>PRT

＜213〉the pathogenic mutation of rice Xanthomonas paddy rice

<400>22

Met?Asn?Ser?Leu?Asn?Thr?Gln?Phe?Gly?Gly?Ser?Thr?Ser?Asn?Leu?Gln

1???????????????5???????????????????10??????????????????15

Val?Gly?Pro?Ser?Gln?Asp?Thr?Thr?Phe?Gly?Ser?Asn?Gln?Gly?Gly?Asn

20??????????????????25??????????????????30

Gln?Gly?Ile?Ser?Glu?Lys?Gln?Leu?Asp?Gln?Leu?Leu?Cys?Gln?Leu?Ile

35??????????????????40??????????????????45

Ser?Ala?Leu?Leu?Gln?Ser?Ser?Lys?Asn?Ala?Glu?Glu?Gly?Lys?Gly?Gln

50??????????????????55??????????????????60

Gly?Gly?Asp?Asn?Gly?Gly?Gly?Gln?Gly?Gly?Asn?Ser?Gln?Gln?Ala?Gly

65??????????????????70??????????????????75??????????????????80

Gln?Gln?Asn?Gly?Pro?Ser?Pro?Phe?Thr?Gln?Met?Leu?Met?His?Ile?Val

85??????????????????90??????????????????95

Gly?Glu?Ile?Leu?Gln?Ala?Gln?Asn?Gly?Gly?Gly?Ala?Gly?Gly?Gly?Gly

100?????????????????105?????????????????110

Phe?Gly?Gly?Gly?Phe?Gly?Gly?Asp?Phe?Ser?Gly?Asp?Leu?Gly?Leu?Gly

115?????????????????120?????????????????125

Thr?Asn?Leu?Ser?Ser?Asp?Ser?Ala?Ser?Met?Gln

130?????????????????135

<210>23

<211>420

<212>DNA

＜213〉the pathogenic mutation of rice Xanthomonas paddy rice

<400>23

atgaattctt?tgaacacaca?attcggcggc?agcacgtcca?accttcaggt?tggcccaagc?????60

caggacacaa?cgttcggttc?gaaccagggc?ggcaaccagg?gcatctcgga?aaagcaactg????120

gaccagttgc?tgtgccagct?catctcggcc?ctgcttcagt?cgagcaaaaa?tgctgaggag????180

ggtaagggtc?agggtggcga?taatggcggt?ggccagggcg?gcaattcgca?gcaggccggg????240

cagcagaatg?gcccctcgcc?attcacccag?atgctgatgc?atatcgtcgg?agagattctc????300

caggcgcaga?atggtggtgg?tgctggtggc?ggcgggttcg?gcggcgggtt?cggcggtgac????360

tttagtggcg?acctcggcct?cggcaccaac?ctctcgagcg?acagcgcatc?gatgcagtaa????420

<210>24

<211>139

<212>PRT

＜213〉the pathogenic mutation of rice Xanthomonas paddy rice

<400>24

Met?Asn?Ser?Leu?Asn?Thr?Gln?Phe?Gly?Gly?Ser?Thr?Ser?Asn?Leu?Gln

1???????????????5???????????????????10??????????????????15

Val?Gly?Pro?Ser?Gln?Asp?Thr?Thr?Phe?Gly?Ser?Asn?Gln?Gly?Gly?Asn

20??????????????????25??????????????????30

Gln?Gly?Ile?Ser?Glu?Lys?Gln?Leu?Asp?Gln?Leu?Leu?Cys?Gln?Leu?Ile

35??????????????????40??????????????????45

Ser?Ala?Leu?Leu?Gln?Ser?Ser?Lys?Asn?Ala?Glu?Glu?Gly?Lys?Gly?Gln

50??????????????????55??????????????????60

Gly?Gly?Asp?Asn?Gly?Gly?Gly?Gln?Gly?Gly?Asn?Ser?Gln?Gln?Ala?Gly

65??????????????????70??????????????????75??????????????????80

Gln?Gln?Asn?Gly?Pro?Ser?Pro?Phe?Thr?Gln?Met?Leu?Met?His?Ile?Val

85??????????????????90??????????????????95

Gly?Glu?Ile?Leu?Gln?Ala?Gln?Asn?Gly?Gly?Gly?Ala?Gly?Gly?Gly?Gly

100?????????????????105?????????????????110

Phe?Gly?Gly?Gly?Phe?Gly?Gly?Asp?Phe?Ser?Gly?Asp?Leu?Gly?Leu?Gly

115?????????????????120?????????????????125

Thr?Asn?Leu?Ser?Ser?Asp?Ser?Ala?Ser?Met?Gln

130?????????????????135

<210>25

<211>378

<212>DNA

＜213〉the rice Xanthomonas rice mutation of causing a disease of dwelling

<400>25

atgaattctt?tgaacacaca?attcggcggc?agcgcgtcca?acttccaggt?tgaccaaagc?????60

cagaacgcgc?aatccgattc?gagccagggc?agcaatggca?gccagggtat?ctcggaaaag????120

caactggacc?agttgctgtg?ccagctcatc?caggccctgc?ttcagccgaa?caaaaatgct????180

gaggaaggta?agggtcagca?gggtggcgag?aataatcagc?aggccgggaa?ggagaatggc????240

gcctcgccac?tcacccagat?gctgatgaat?atcgtcggag?agattctcca?ggcgcagaat????300

gccggcggca?gcagcggcgg?cgactttggt?ggcagtttcg?ccagcagctt?ctcgaacgac????360

agcggatcga?tgcagtaa??????????????????????????????????????????????????378

<210>26

<211>125

<212>PRT

＜213〉the rice Xanthomonas rice mutation of causing a disease of dwelling

<400>26

Met?Asn?Ser?Leu?Asn?Thr?Gln?Phe?Gly?Gly?Ser?Ala?Ser?Asn?Phe?Gln

1???????????????5???????????????????10??????????????????15

Val?Asp?Gln?Ser?Gln?Asn?Ala?Gln?Ser?Asp?Ser?Ser?Gln?Gly?Ser?Asn

20??????????????????25??????????????????30

Gly?Ser?Gln?Gly?Ile?Ser?Glu?Lys?Gln?Leu?Asp?Gln?Leu?Leu?Cys?Gln

35??????????????????40??????????????????45

Leu?Ile?Gln?Ala?Leu?Leu?Gln?Pro?Asn?Lys?Asn?Ala?Glu?Glu?Gly?Lys

50??????????????????55??????????????????60

Gly?Gln?Gln?Gly?Gly?Glu?Asn?Asn?Gln?Gln?Ala?Gly?Lys?Glu?Asn?Gly

65??????????????????70??????????????????75??????????????????80

Ala?Ser?Pro?Leu?Thr?Gln?Met?Leu?Met?Asn?Ile?Val?Gly?Glu?Ile?Leu

85??????????????????90??????????????????95

Gln?Ala?Gln?Asn?Ala?Gly?Gly?Ser?Ser?Gly?Gly?Asp?Phe?Gly?Gly?Ser

100??????????????????105??????????????????110

Phe?Ala?Ser?Ser?Phe?Ser?Asn?Asp?Ser?Gly?Ser?Met?Gln

115?????????????????120?????????????????125

<210>27

<211>366

<212>DNA

＜213〉the pathogenic mutation of cabbage black rot bacterium wild cabbage

<400>27

tcaggcttgg?ccggtgatgc?tcgacaggtt?ggcattgaag?ccgccaccca?agctggtgcc?????60

gcccatgccg?gcgccgcctt?ggttctgcat?cagctgcatc?acgatctgca?tcagcatctg????120

cgtcaacgga?ctcacaccgt?cctgttgacc?gctctgcggt?tgttcgtctc?cgcactcctg????180

atcggcatcg?ctgccctggc?tctgttggag?catcatcatg?atgaacatgg?cgagcagctg????240

atccagctgc?tgctcggagt?cagccgaagg?cgagcgctga?ctggagttct?gggtttgctg????300

gggcccgatg?cccatcgtct?gcaggttgat?gaagttggaa?aatttgtttc?cgatagatga????360

gtccat???????????????????????????????????????????????????????????????366

<210>28

<211>121

<212>PRT

＜213〉the pathogenic mutation of cabbage black rot bacterium wild cabbage

<400>28

Met?Asp?Ser?Ser?Ile?Gly?Asn?Lys?Phe?Ser?Asn?Phe?Ile?Asn?Leu?Gln

1???????????????5???????????????????10??????????????????15

Thr?Met?Gly?Ile?Gly?Pro?Gln?Gln?Thr?Gln?Asn?Ser?Ser?Gln?Arg?Ser

20??????????????????25??????????????????30

Pro?Ser?Ala?Asp?Ser?Glu?Gln?Gln?Leu?Asp?Gln?Leu?Leu?Ala?Met?Phe

35??????????????????40??????????????????45

Ile?Met?Met?Met?Leu?Gln?Gln?Ser?Gln?Gly?Ser?Asp?Ala?Asp?Gln?Glu

50??????????????????55??????????????????60

Cys?Gly?Asp?Glu?Gln?Pro?Gln?Ser?Gly?Gln?Gln?Asp?Gly?Val?Ser?Pro

65??????????????????70??????????????????75??????????????????80

Leu?Thr?Gln?Met?Leu?Met?Gln?Ile?Val?Met?Gln?Leu?Met?Gln?Asn?Gln

85??????????????????90??????????????????95

Gly?Gly?Ala?Gly?Met?Gly?Gly?Thr?Ser?Leu?Gly?Gly?Gly?Phe?Asn?Ala

100?????????????????105?????????????????110

Asn?Leu?Ser?Ser?Ile?Thr?Gly?Gln?Ala

115?????????????????120

<210>29

<211>3282

<212>DNA

＜213〉cytoalgae

<400>29

tgttcgttgc?acaaattgat?gagcaatgct?tttttataat?gccaactttg?tacaaaaaag??????60

caggcttaaa?caatggcgac?tatccacggt?aattggcaac?cctcccacgg?ggaaaacggc?????120

ggcaaactgt?ttctttgggc?ggatacctgg?ggtcatcctt?tgccagaaac?cattggcgat?????180

cgccatccct?ttgcgttgga?tctgccggat?ttgctacagg?cctggtcgaa?tttgcccctg?????240

gccttcccca?aggcggatgg?ggtgacagag?gcagccctta?ctctgcattt?acccagccat?????300

cgccagcaaa?aaattcccct?accctttgtc?acagggcaag?atccggtggc?catggatgcg?????360

aaatatctcc?actggcgatc?gtggcaggta?accggggtaa?atctgacccc?aagccaaacg?????420

ttaacgttgc?tccaatctat?tcccctgggg?ggccaagcct?tagctaactt?aggatcagag?????480

ttttactttt?acggtcaact?gcaccgctgg?tgtttagatt?tggtgctacg?gggtaaattt?????540

gtgccgggac?tggagcaaag?gggggaagac?ggtaattact?atgcccaatg?gattcctatc?????600

ctcgatagca?tccaagacca?aacccattta?gcccaattta?gccagagagt?acctgcctgc?????660

gccctggcca?acctgactga?ctcccaggag?ccccaaatgt?tggtggtgga?tttactacaa?????720

aaattattgc?aagcccaaat?tggtgccgtc?agtcccagcc?tagccaacgt?taaagaagtc?????780

tggttgaatg?attggctccg?gggattaacc?catggggggc?aaacctccct?cggcacaagc?????840

aaagctctac?aacgattagc?cacatcctta?gaccattggt?atttaccagt?ccagaattat?????900

ttgggccaaa?aaaataacca?agctttagcc?caacggcaat?ggcggggggc?tctgcggtta?????960

caacctccag?cggacgatgg?ggggggaacc?tggcaactgg?attatggttt?acaagccctg????1020

gatgacgggg?aattttggct?cccggcggct?tccctctggg?ccatggccgg?cgatcgcctg????1080

gtgtggcagg?gaaggagggt?tgaccagggg?gcggaaagtt?tactgcgggg?cttaggggta????1140

gctgcccaaa?tttacgaacc?cattgctgca?agtttgacgg?aaaggtgtcc?cacgggctgt????1200

gggctagatg?ccatccaagc?ctacgaattt?atcctggcaa?tcgcccatca?attgcgggat????1260

cgggggttag?gggtaatcct?cccgccgggg?ttagaacggg?gcggcaccgc?caaacggtta????1320

ggggtaaaag?tggtggggga?agtgcaacgg?caaaggggcc?agcggctaac?tctgcaaagt????1380

ttaattaatt?acgacttgca?actaatgatg?gggagcgggg?acaatgcccg?gttattgacg????1440

gccaaggact?ttgaagcgtt?actagcccaa?aaatctcccc?tggtggtgct?ggacggagaa????1500

tggattaccc?tgcaaccggc?ggacgtgcgg?gcggccaagg?tcattttaca?gcagcaacaa????1560

tctgccccgc?ccctcacagt?ggaggatgct?ctgcgcctca?gcattggtga?tttacaaacc????1620

gtctctaaac?tgccggtgac?ccagtttgct?gctcggggca?tattacagga?attgatcgac????1680

accctccgta?acccggaagg?agtgaaagcc?attgctgacc?caccgggctt?tcagggtact????1740

ttacggccct?accaagctcg?gggagtgggc?tggttagctt?ttctggaacg?gtgggggctg????1800

ggggcctgtt?tggcagacga?tatgggtttg?ggaaaaacac?cccagttgct?ggcttttctg????1860

ctccatttag?ccgcggagga?tatgttagtt?aagccggtgt?tgattgtttg?tcctacgtcg????1920

gtgctgagca?attggggtca?tgaaattaat?aagtttgcgc?cccaacttaa?aaccctattg????1980

caccatggcg?atcgccggaa?aaaagggcaa?ccgttggtta?aacaggtcaa?agaccagcaa????2040

attgtcctca?ccagttacgc?tttactgcaa?cgggatttta?gtagtttgaa?attggtggac????2100

tggcagggga?tcgtgctgga?cgaagcccaa?aatatcaaaa?atccccaagc?taaacagtcc????2160

caggcggccc?ggcaattgcc?agcgggtttt?cgcattgccc?tcacggggac?tccggtggaa????2220

aatcgcctga?cggaattgtg?gtcaatttta?gaatttttaa?atcccggttt?cctgggtaat????2280

cagagctttt?tccaacggcg?ctttgccaat?cccatcgaaa?aatttggcga?tcgccagtcg????2340

ttgttaattt?tgcggaattt?agtgcggccg?tttattttgc?ggcggttaaa?aaccgaccaa????2400

accattattc?aagatttacc?agaaaaacaa?gaaatgaccg?tcttctgtga?cctttcccaa????2460

gagcaagctg?gtttatatca?acaattggtg?gaggaatccc?tccaggcgat?cgccgacagc????2520

gaaggcattc?aaaggcacgg?tttagtttta?accctattaa?ccaaactcaa?acaggtttgt????2580

aaccatcccg?atctattgct?gaaaaagccc?gccatcaccc?acgggcacca?gtccggcaag??2640

ctaattcgtc?tggcggaaat?gctggaagaa?atcatcagcg?aaggcgatcg?ggtgttaatt??2700

ttcacccaat?ttgccagttg?gggtcattta?ctcaaaccct?atctggaaaa?atactttaac??2760

caagaggtgc?tctatctcca?cgggggcact?ccagcagagc?aacggcaagc?tctggtggaa??2820

cgattccaac?aggaccccaa?cagtccctat?ttatttatcc?tttctctcaa?ggctggcggc??2880

acagggttga?acctcacgag?ggctaaccat?gtgttccatg?tggaccggtg?gtggaatccg??2940

gcggtggaaa?atcaggctac?cgatcgtgct?tttcgcattg?gccaaactcg?caacgtccag??3000

gtgcacaaat?ttgtctgtac?aggcaccttg?gaagaaaaaa?ttaacgccat?gatggcggat??3060

aaacaacaat?tggcagaaca?aaccgtggat?gccggggaaa?attggctcac?ccgcctagac??3120

accgataaac?tccgtcagtt?gcttaccctc?tccgccaccc?cggtggatta?ccaagccgaa??3180

gcgtccgatt?gaacccagct?ttcttgtaca?aagttggcat?gataagaaag?cattgcttat??3240

caatttgttg?caacgaacag?gtcactatca?gtcaaaataa?at?????????????????????3282

<210>30

<211>1039

<212>PRT

＜213〉cytoalgae

<400>30

Met?Ala?Thr?Ile?His?Gly?Asn?Trp?Gln?Pro?Ser?His?Gly?Glu?Asn?Gly

1???????????????5???????????????????10??????????????????15

Gly?Lys?Leu?Phe?Leu?Trp?Ala?Asp?Thr?Trp?Gly?His?Pro?Leu?Pro?Glu

20??????????????????25??????????????????30

Thr?Ile?Gly?Asp?Arg?His?Pro?Phe?Ala?Leu?Asp?Leu?Pro?Asp?Leu?Leu

35??????????????????40??????????????????45

Gln?Ala?Trp?Ser?Asn?Leu?Pro?Leu?Ala?Phe?Pro?Lys?Ala?Asp?Gly?Val

50??????????????????55??????????????????60

Thr?Glu?Ala?Ala?Leu?Thr?Leu?Hi?s?Leu?Pro?Ser?His?Arg?Gln?Gln?Lys

65??????????????????70??????????????????75??????????????????80

Ile?Pro?Leu?Pro?Phe?Val?Thr?Gly?Gln?Asp?Pro?Val?Ala?Met?Asp?Ala

85??????????????????90??????????????????95

Lys?Tyr?Leu?His?Trp?Arg?Ser?Trp?Gln?Val?Thr?Gly?Val?Asn?Leu?Thr

100?????????????????105?????????????????110

Pro?Ser?Gln?Thr?Leu?Thr?Leu?Leu?Gln?Ser?Ile?Pro?Leu?Gly?Gly?Gln

115?????????????????120?????????????????125

Ala?Leu?Ala?Asn?Leu?Gly?Ser?Glu?Phe?Tyr?Phe?Tyr?Gly?Gln?Leu?His

130?????????????????135?????????????????140

Arg?Trp?Cys?Leu?Asp?Leu?Val?Leu?Arg?Gly?Lys?Phe?Val?Pro?Gly?Leu

145?????????????????150?????????????????155?????????????????160

Glu?Gln?Arg?Gly?Glu?Asp?Gly?Asn?Tyr?Tyr?Ala?Gln?Trp?Ile?Pro?Ile

165?????????????????170?????????????????175

Leu?Asp?Ser?Ile?Gln?Asp?Gln?Thr?His?Leu?Ala?Gln?Phe?Ser?Gln?Arg

180?????????????????185?????????????????190

Val?Pro?Ala?Cys?Ala?Leu?Ala?Asn?Leu?Thr?Asp?Ser?Gln?Glu?Pro?Gln

195?????????????????200?????????????????205

Met?Leu?Val?Val?Asp?Leu?Leu?Gln?Lys?Leu?Leu?Gln?Ala?Gln?Ile?Gly

210?????????????????215?????????????????220

Ala?Val?Ser?Pro?Ser?Leu?Ala?Asn?Val?Lys?Glu?Val?Trp?Leu?Asn?Asp

225?????????????????230?????????????????235?????????????????240

Trp?Leu?Arg?Gly?Leu?Thr?His?Gly?Gly?Gln?Thr?Ser?Leu?Gly?Thr?Ser

245?????????????????250?????????????????255

Lys?Ala?Leu?Gln?Arg?Leu?Ala?Thr?Ser?Leu?Asp?His?Trp?Tyr?Leu?Pro

260?????????????????265?????????????????270

Val?Gln?Asn?Tyr?Leu?Gly?Gln?Lys?Asn?Asn?Gln?Ala?Leu?Ala?Gln?Arg

275?????????????????280?????????????????285

Gln?Trp?Arg?Gly?Ala?Leu?Arg?Leu?Gln?Pro?Pro?Ala?Asp?Asp?Gly?Gly

290?????????????????295?????????????????300

Gly?Thr?Trp?Gln?Leu?Asp?Tyr?Gly?Leu?Gln?Ala?Leu?Asp?Asp?Gly?Glu

305?????????????????310?????????????????315?????????????????320

Phe?Trp?Leu?Pro?Ala?Ala?Ser?Leu?Trp?Ala?Met?Ala?Gly?Asp?Arg?Leu

325?????????????????330?????????????????335

Val?Trp?Gln?Gly?Arg?Arg?Val?Asp?Gln?Gly?Ala?Glu?Ser?Leu?Leu?Arg

340?????????????????345?????????????????350

Gly?Leu?Gly?Val?Ala?Ala?Gln?Ile?Tyr?Glu?Pro?Ile?Ala?Ala?Ser?Leu

355?????????????????360?????????????????365

Thr?Glu?Arg?Cys?Pro?Thr?Gly?Cys?Gly?Leu?Asp?Ala?Ile?Gln?Ala?Tyr

370?????????????????375?????????????????380

Glu?Phe?Ile?Leu?Ala?Ile?Ala?His?Gln?Leu?Arg?Asp?Arg?Gly?Leu?Gly

385?????????????????390?????????????????395?????????????????400

Val?Ile?Leu?Pro?Pro?Gly?Leu?Glu?Arg?Gly?Gly?Thr?Ala?Lys?Arg?Leu

405?????????????????410?????????????????415

Gly?Val?Lys?Val?Val?Gly?Glu?Val?Gln?Arg?Gln?Arg?Gly?Gln?Arg?Leu

420?????????????????425?????????????????430

Thr?Leu?Gln?Ser?Leu?Ile?Asn?Tyr?Asp?Leu?Gln?Leu?Met?Met?Gly?Ser

435?????????????????440?????????????????445

Gly?Asp?Asn?Ala?Arg?Leu?Leu?Thr?Ala?Lys?Asp?Phe?Glu?Ala?Leu?Leu

450?????????????????455?????????????????460

Ala?Gln?Lys?Ser?Pro?Leu?Val?Val?Leu?Asp?Gly?Glu?Trp?Ile?Thr?Leu

465?????????????????470?????????????????475?????????????????480

Gln?Pro?Ala?Asp?Val?Arg?Ala?Ala?Lys?Val?Ile?Leu?Gln?Gln?Gln?Gln

485?????????????????490?????????????????495

Ser?Ala?Pro?Pro?Leu?Thr?Val?Glu?Asp?Ala?Leu?Arg?Leu?Ser?Ile?Gly

500?????????????????505?????????????????510

Asp?Leu?Gln?Thr?Val?Ser?Lys?Leu?Pro?Val?Thr?Gln?Phe?Ala?Ala?Arg

515?????????????????520?????????????????525

Gly?Ile?Leu?Gln?Glu?Leu?Ile?Asp?Thr?Leu?Arg?Asn?Pro?Glu?Gly?Val

530?????????????????535?????????????????540

Lys?Ala?Ile?Ala?Asp?Pro?Pro?Gly?Phe?Gln?Gly?Thr?Leu?Arg?Pro?Tyr

545?????????????????550?????????????????555?????????????????560

Gln?Ala?Arg?Gly?Val?Gly?Trp?Leu?Ala?Phe?Leu?Glu?Arg?Trp?Gly?Leu

565?????????????????570?????????????????575

Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Pro?Gln?Leu

580?????????????????585?????????????????590

Leu?Ala?Phe?Leu?Leu?His?Leu?Ala?Ala?Glu?Asp?Met?Leu?Val?Lys?Pro

595?????????????????600?????????????????605

Val?Leu?Ile?Val?Cys?Pro?Thr?Ser?Val?Leu?Ser?Asn?Trp?Gly?His?Glu

610?????????????????615?????????????????620

Ile?Asn?Lys?Phe?Ala?Pro?Gln?Leu?Lys?Thr?Leu?Leu?His?His?Gly?Asp

625?????????????????630?????????????????635?????????????????640

Arg?Arg?Lys?Lys?Gly?Gln?Pro?Leu?Val?Lys?Gln?Val?Lys?Asp?Gln?Gln

645?????????????????650?????????????????655

Ile?Val?Leu?Thr?Ser?Tyr?Ala?Leu?Leu?Gln?Arg?Asp?Phe?Ser?Ser?Leu

660?????????????????665?????????????????670

Lys?Leu?Val?Asp?Trp?Gln?Gly?Ile?Val?Leu?Asp?Glu?Ala?Gln?Asn?Ile

675?????????????????680?????????????????685

Lys?Asn?Pro?Gln?Ala?Lys?Gln?Ser?Gln?Ala?Ala?Arg?Gln?Leu?Pro?Ala

690?????????????????695?????????????????700

Gly?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu?Thr

705?????????????????710?????????????????715?????????????????720

Glu?Leu?Trp?Ser?Ile?Leu?Glu?Phe?Leu?Asn?Pro?Gly?Phe?Leu?Gly?Asn

725?????????????????730?????????????????735

Gln?Ser?Phe?Phe?Gln?Arg?Arg?Phe?Ala?Asn?Pro?Ile?Glu?Lys?Phe?Gly

740?????????????????745?????????????????750

Asp?Arg?Gln?Ser?Leu?Leu?Ile?Leu?Arg?Asn?Leu?Val?Arg?Pro?Phe?Ile

755?????????????????760?????????????????765

Leu?Arg?Arg?Leu?Lys?Thr?Asp?Gln?Thr?IleIle?Gln?Asp?Leu?Pro?Glu

770?????????????????775?????????????????780

Lys?Gln?Glu?Met?Thr?Val?Phe?Cys?Asp?Leu?Ser?Gln?Glu?Gln?Ala?Gly

785?????????????????790?????????????????795?????????????????800

Leu?Tyr?Gln?Gln?Leu?Val?Glu?Glu?Ser?Leu?Gln?Ala?Ile?Ala?Asp?Ser

805?????????????????810?????????????????815

Glu?Gly?Ile?Gln?Arg?His?Gly?Leu?Val?Leu?Thr?Leu?Leu?Thr?Lys?Leu

820?????????????????825?????????????????830

Lys?Gln?Val?Cys?Asn?His?Pro?Asp?Leu?Leu?Leu?Lys?Lys?Pro?Ala?Ile

835?????????????????840?????????????????845

Thr?His?Gly?His?Gln?Ser?Gly?Lys?Leu?Ile?Arg?Leu?Ala?Glu?Met?Leu

850?????????????????855?????????????????860

Glu?Glu?Ile?Ile?Ser?Glu?Gly?Asp?Arg?Val?Leu?Ile?Phe?Thr?Gln?Phe

865?????????????????870?????????????????875?????????????????880

Ala?Ser?Trp?Gly?His?Leu?Leu?Lys?Pro?Tyr?Leu?Glu?Lys?Tyr?Phe?Asn

885?????????????????890?????????????????895

Gln?Glu?Val?Leu?Tyr?Leu?His?Gly?Gly?Thr?Pro?Ala?Glu?Gln?Arg?Gln

900?????????????????905?????????????????910

Ala?Leu?Val?Glu?Arg?Phe?Gln?Gln?Asp?Pro?Asn?Ser?Pro?Tyr?Leu?Phe

915?????????????????920?????????????????925

Ile?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Thr?Gly?Leu?Asn?Leu?Thr?Arg?Ala

930?????????????????935?????????????????940

Asn?His?Val?Phe?His?Val?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn

945?????????????????950?????????????????955?????????????????960

Gln?Ala?Thr?Asp?Arg?Ala?Phe?Arg?Ile?Gly?Gln?Thr?Arg?Asn?Val?Gln

965?????????????????970?????????????????975

Val?His?Lys?Phe?Val?Cys?Thr?Gly?Thr?Leu?Glu?Glu?Lys?Ile?Asn?Ala

980?????????????????985?????????????????990

Met?Met?Ala?Asp?Lys?Gln?Gln?Leu??Ala?Glu?Gln?Thr?Val??Asp?Ala?Gly

995?????????????????1000?????????????????1005

Glu?Asn??Trp?Leu?Thr?Arg?Leu??Asp?Thr?Asp?Lys?Leu??Arg?Gln?Leu

1010?????????????????1015?????????????????1020

Leu?Thr??Leu?Ser?Ala?Thr?Pro??Val?Asp?Tyr?Gln?Ala??Glu?Ala?Ser

1025?????????????????1030?????????????????1035

Asp

<210>31

<211>3237

<212>DNA

<213>Anaebena?variabilis

<400>31

atggcaattt?tacacggtag?ttggatatta?agtgagcagg?atagttattt?atttatttgg?????60

ggggaaactt?ggcgatcgcc?acaagtaaat?tttagttttg?aggaaatagc?cctcaatccc????120

ttggctctgt?ctgcatctga?attaagcgag?tggttgcagt?ctcaacatca?ggcgatcgct????180

cagattttac?cacaacagtt?ggcaaaaaaa?acctccaaag?cagcaagttc?cccaacaaca????240

aatttaccaa?ttcactcgca?aataattgtt?ctgccaacgg?aaatttctca?acctcgtaag????300

aaagaaacaa?ttttcatttc?tcctgtgcat?tctgccgctt?tagaatctga?tgcagactct????360

gaagtttatt?tacaaccttg?gcgtgtagaa?ggtttttgtc?ttcctcctag?tgcagcagtt????420

aaatttctaa?cttctttacc?tttaaatatc?actagcacag?agaatgcttt?tttaggtgga????480

gatttacgtt?tttggtcaca?aattgcccgt?tggagtttag?atttaatttc?taggtctaag????540

tttctcccaa?ttatccaacg?acaacctaat?aattctgtaa?gtgccaaatg?gcaagtactg????600

ttagatagtg?ctgtagatgg?aactcgttta?gaaaagttcg?ccgcgaagat?gcctttggtt????660

tgtcggactt?atcagagatt?agggaacgag?gaattatctc?catctcctat?atatatagat????720

tttcctagtc?agccgcagga?attaatattg?ggttttctca?atagtgcaat?agatacgcaa????780

ttacgggaaa?tggtggggaa?tcagcctgtg?gtggaaactc?gcttgatggc?atctttaccg????840

tcggcggtac?gacagtggct?gcaagggtta?agtggtgcat?ctaattcagt?tgatgcagat????900

gcagttggtt?tggaaaggct?ggaagcagcg?ctcaaggctt?ggacgatgcc?gctacaatat????960

caactagcaa?gtaaaaatca?atttcgcacc?tgttttgaat?tacgttctcc?agaaccagga???1020

gaaactgaat?ggacactagc?ctatttcctg?caagcagccg?ataatccaga?atttctagta???1080

gatgcgggca?ctatttggca?acatcctgtt?gaacagctaa?tttatcaaca?gcgatcgatt???1140

caagaacccc?aggaaacatt?tttacgaggt?ttggggttag?cttctcgatt?gtatccggtc???1200

attgccccca?ctttagatac?agaatcaccg?caattttgtc?atctcaaccc?catgcaggct???1260

tatgaattta?tcaaggctgt?ggcttggcga?tttgaagata?gcggtttagg?ggtgatttta???1320

cctcctagtt?tggcgaaccg?ggaaggctgg?gcaaaccgct?tgggattgaa?aatctccgcc???1380

gaaaccccaa?agaaaaagcc?aggacgcttg?ggattgcaga?gtttgcttaa?ttttcaatgg???1440

cacttagcaa?ttggtgggca?aactatttct?aaaggggaat?ttgacagact?agtagcttta???1500

aaaagcccat?tggtagaaat?aaatggcgaa?tgggtggagt?tgcgtcccca?agatatcaag????1560

acagccgaag?ccttttttgc?tgcacgtaaa?gaccaaatgg?ccttatcttt?agaagatgct????1620

ttacgtctga?gtagtgggga?tactcaagta?attgagaaat?taccagtagt?cagctttgaa????1680

gcctctggcg?cattacaaga?attaattggg?gcgctgacaa?ataatcaagc?agttgcacca????1740

ttacctacgc?caaagaactt?ccaaggaaag?ttgcgtcctt?atcaagaaag?gggtgcggct????1800

tggttggcat?tcctcgaacg?ctggggttta?ggtgcttgtc?tcgccgacga?catgggactg????1860

ggaaaaacga?tacagttcat?tgctttcctt?ctccatctta?aagaacagga?tgtattagaa????1920

aaaccaactt?tactagtgtg?tcctacttct?gttttaggta?actgggaacg?agaagtgaaa????1980

aaatttgcac?ctacacttaa?agttctccaa?tatcatggtg?ataaacgtcc?taaaggtaaa????2040

gcttttccag?aagcagtaaa?aaatcatgat?ttagttatca?ccagttactc?actaattcat????2100

agagacatca?aatcattgca?gggtctttct?tggcagataa?ttgttttaga?tgaagcccag????2160

aatgtgaaga?atgcggaagc?caaacaatca?caagcagtcc?gacaattaga?cacaaccttt????2220

cgcattgctt?taacggggac?accagtcgaa?aatagactac?aggaactttg?gtcaatttta????2280

gatttcctca?accctggtta?tttaggtaat?aagcaattct?tccaaagacg?ctttgccatg????2340

ccaattgaaa?agtatggtga?tgcagcatct?ttaaatcaat?tgcgtgcctt?agtacaacca????2400

tttattctgc?gtcgcctgaa?aacagaccgt?gatattattc?aagacttgcc?agataagcaa????2460

gaaatgacag?tattttgcgg?tttgactgga?gaacaagctg?cactttatca?aaaagtggta????2520

gaaacatctt?tagcagaaat?tgaatcggcc?gaaggattgc?aacgccgagg?gatgatttta????2580

gctttattaa?ttaaactcaa?acaaatctgc?aatcatccag?cccaatatct?gaaaacaaat????2640

accttagaac?aatacagttc?aggaaaactg?caacgattag?aagaaatgtt?agaagaggtg????2700

ttagcggaga?gtaatactta?tggtgttgct?ggtgcgggac?gtgctttaat?cttcacccag????2760

tttgcagaat?ggggtaagtt?actcaaacca?catttagaaa?aacaactagg?gcgggaagta????2820

tttttcttat?atggtagtac?cagtaaaaag?caacgtgaag?aaatgattga?ccgttttcaa????2880

cacgaccctc?aggggccacc?aattatgatt?ctctctctca?aagcaggtgg?tgtagggttg????2940

aacttaacca?gagcaaatca?tgtatttcac?tttgatagat?ggtggaatcc?agccgtagag????3000

aaccaagcca?cagaccgcgt?atttcgtatt?ggtcaaaccc?gcaatgtaca?ggtgcataaa????3060

tttgtttgca?atggtacctt?agaagaaaaa?atccacgaca?tgattgaaag?taaaaaacaa????3120

ctagcggaac?aggttgttgg?tgcaggcgaa?gagtggttaa?ctgaattaga?tacagatcaa????3180

ctccgcaact?tactgatact?tgatcgtagt?gcagtaattg?atgaagaagc?agagtaa???????3237

<210>32

<211>1078

<212>PRT

<213>Anaebena?variabilis

<400>32

Met?Ala?Ile?Leu?His?Gly?Ser?Trp?Ile?Leu?Ser?Glu?Gln?Asp?Ser?Tyr

1???????????????5???????????????????10??????????????????15

Leu?Phe?Ile?Trp?Gly?Glu?Thr?Trp?Arg?Ser?Pro?Gln?Val?Asn?Phe?Ser

20??????????????????25??????????????????30

Phe?Glu?Glu?Ile?Ala?Leu?Asn?Pro?Leu?Ala?Leu?Ser?Ala?Ser?Glu?Leu

35??????????????????40??????????????????45

Ser?Glu?Trp?Leu?Gln?Ser?Gln?His?Gln?Ala?Ile?Ala?Gln?Ile?Leu?Pro

50??????????????????55??????????????????60

Gln?Gln?Leu?Ala?Lys?Lys?Thr?Ser?Lys?Ala?Ala?Ser?Ser?Pro?Thr?Thr

65??????????????????70??????????????????75??????????????????80

Asn?Leu?Pro?Ile?His?Ser?Gln?Ile?Ile?Val?Leu?Pro?Thr?Glu?Ile?Ser

85??????????????????90??????????????????95

Gln?Pro?Arg?Lys?Lys?Glu?Thr?Ile?Phe?Ile?Ser?Pro?Val?His?Ser?Ala

100?????????????????105?????????????????110

Ala?Leu?Glu?Ser?Asp?Ala?Asp?Ser?Glu?Val?Tyr?Leu?Gln?Pro?Trp?Arg

115?????????????????120?????????????????125

Val?Glu?Gly?Phe?Cys?Leu?Pro?Pro?Ser?Ala?Ala?Val?Lys?Phe?Leu?Thr

130?????????????????135?????????????????140

Ser?Leu?Pro?Leu?Asn?Ile?Thr?Ser?Thr?Glu?Asn?Ala?Phe?Leu?Gly?Gly

145?????????????????150?????????????????155?????????????????160

Asp?Leu?Arg?Phe?Trp?Ser?Gln?Ile?Ala?Arg?Trp?Ser?Leu?Asp?Leu?Ile

165?????????????????170?????????????????175

Ser?Arg?Ser?Lys?Phe?Leu?Pro?Ile?Ile?Gln?Arg?Gln?Pro?Asn?Asn?Ser

180?????????????????185?????????????????190

Val?Ser?Ala?Lys?Trp?Gln?Val?Leu?Leu?Asp?Ser?Ala?Val?Asp?Gly?Thr

195?????????????????200?????????????????205

Arg?Leu?Glu?Lys?Phe?Ala?Ala?Lys?Met?Pro?Leu?Val?Cys?Arg?Thr?Tyr

210?????????????????215?????????????????220

Gln?Arg?Leu?Gly?Asn?Glu?Glu?Leu?Ser?Pro?Ser?Pro?Ile?Tyr?Ile?Asp

225?????????????????230?????????????????235?????????????????240

Phe?Pro?Ser?Gln?Pro?Gln?Glu?Leu?Ile?Leu?Gly?Phe?Leu?Asn?Ser?Ala

245?????????????????250?????????????????255

Ile?Asp?Thr?Gln?Leu?Arg?Glu?Met?Val?Gly?Asn?Gln?Pro?Val?Val?Glu

260?????????????????265?????????????????270

Thr?Arg?Leu?Met?Ala?Ser?Leu?Pro?Ser?Ala?Val?Arg?Gln?Trp?Leu?Gln

275?????????????????280?????????????????285

Gly?Leu?Ser?Gly?Ala?Ser?Asn?Ser?Val?Asp?Ala?Asp?Ala?Val?Gly?Leu

290?????????????????295?????????????????300

Glu?Arg?Leu?Glu?Ala?Ala?Leu?Lys?Ala?Trp?Thr?Met?Pro?Leu?Gln?Tyr

305?????????????????310?????????????????315?????????????????320

Gln?Leu?Ala?Ser?Lys?Asn?Gln?Phe?Arg?Thr?Cys?Phe?Glu?Leu?Arg?Ser

325?????????????????330?????????????????335

Pro?Glu?Pro?Gly?Glu?Thr?Glu?Trp?Thr?Leu?Ala?Tyr?Phe?Leu?Gln?Ala

340?????????????????345?????????????????350

Ala?Asp?Asn?Pro?Glu?Phe?Leu?Val?Asp?Ala?Gly?Thr?Ile?Trp?Gln?His

355?????????????????360?????????????????365

Pro?Val?Glu?Gln?Leu?Ile?Tyr?Gln?Gln?Arg?Ser?Ile?Gln?Glu?Pro?Gln

370?????????????????375?????????????????380

Glu?Thr?Phe?Leu?Arg?Gly?Leu?Gly?Leu?Ala?Ser?Arg?Leu?Tyr?Pro?Val

385?????????????????390?????????????????395?????????????????400

Ile?Ala?Pro?Thr?Leu?Asp?Thr?Glu?Ser?Pro?Gln?Phe?Cys?His?Leu?Asn

405?????????????????410?????????????????415

Pro?Met?Gln?Ala?Tyr?Glu?Phe?Ile?Lys?Ala?Val?Ala?Trp?Arg?Phe?Glu

420?????????????????425?????????????????430

Asp?Ser?Gly?Leu?Gly?Val?Ile?Leu?Pro?Pro?Ser?Leu?Ala?Asn?Arg?Glu

435?????????????????440?????????????????445

Gly?Trp?Ala?Asn?Arg?Leu?Gly?Leu?Lys?Ile?Ser?Ala?Glu?Thr?Pro?Lys

450?????????????????455?????????????????460

Lys?Lys?Pro?Gly?Arg?Leu?Gly?Leu?Gln?Ser?Leu?Leu?Asn?Phe?Gln?Trp

465?????????????????470?????????????????475?????????????????480

His?Leu?Ala?Ile?Gly?Gly?Gln?Thr?Ile?Ser?Lys?Gly?Glu?Phe?Asp?Arg

485?????????????????490?????????????????495

Leu?Val?Ala?Leu?Lys?Ser?Pro?Leu?Val?Glu?Ile?Asn?Gly?Glu?Trp?Val

500?????????????????505?????????????????510

Glu?Leu?Arg?Pro?Gln?Asp?Ile?Lys?Thr?Ala?Glu?Ala?Phe?Phe?Ala?Ala

515?????????????????520?????????????????525

Arg?Lys?Asp?Gln?Met?Ala?Leu?Ser?Leu?Glu?Asp?Ala?Leu?Arg?Leu?Ser

530?????????????????535?????????????????540

Ser?Gly?Asp?Thr?Gln?Val?Ile?Glu?Lys?Leu?Pro?Val?Val?Ser?Phe?Glu

545?????????????????550?????????????????555?????????????????560

Ala?Ser?Gly?Ala?Leu?Gln?Glu?Leu?Ile?Gly?Ala?Leu?Thr?Asn?Asn?Gln

565?????????????????570?????????????????575

Ala?Val?Ala?Pro?Leu?Pro?Thr?Pro?Lys?Asn?Phe?Gln?Gly?Lys?Leu?Arg

580?????????????????585?????????????????590

Pro?Tyr?Gln?Glu?Arg?Gly?Ala?Ala?Trp?Leu?Ala?Phe?Leu?Glu?Arg?Trp

595?????????????????600?????????????????605

Gly?Leu?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile

610?????????????????615?????????????????620

Gln?Phe?Ile?Ala?Phe?Leu?Leu?His?Leu?Lys?Glu?Gln?Asp?Val?Leu?Glu

625?????????????????630?????????????????635?????????????????640

Lys?Pro?Thr?Leu?Leu?Val?Cys?Pro?Thr?Ser?Val?Leu?Gly?Asn?Trp?Glu

645?????????????????650?????????????????655

Arg?Glu?Val?Lys?Lys?Phe?Ala?Pro?Thr?Leu?Lys?Val?Leu?Gln?Tyr?His

660?????????????????665?????????????????670

Gly?Asp?Lys?Arg?Pro?Lys?Gly?Lys?Ala?Phe?Pro?Glu?Ala?Val?Lys?Asn

675?????????????????680?????????????????685

His?Asp?Leu?Val?Ile?Thr?Ser?Tyr?Ser?Leu?Ile?His?Arg?Asp?Ile?Lys

690?????????????????695?????????????????700

Ser?Leu?Gln?Gly?Leu?Ser?Trp?Gln?Ile?Ile?Val?Leu?Asp?Glu?Ala?Gln

705?????????????????710?????????????????715?????????????????720

Asn?Val?Lys?Asn?Ala?Glu?Ala?Lys?Gln?Ser?Gln?Ala?Val?Arg?Gln?Leu

725?????????????????730?????????????????735

Asp?Thr?Thr?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg

740?????????????????745?????????????????750

Leu?Gln?Glu?Leu?Trp?Ser?Ile?Leu?Asp?Phe?Leu?Asn?Pro?Gly?Tyr?Leu

755?????????????????760?????????????????765

Gly?Asn?Lys?Gln?Phe?Phe?Gln?Arg?Arg?Phe?Ala?Met?Pro?Ile?Glu?Lys

770?????????????????775?????????????????780

Tyr?Gly?Asp?Ala?Ala?Ser?Leu?Asn?Gln?Leu?Arg?Ala?Leu?Val?Gln?Pro

785?????????????????790?????????????????795?????????????????800

Phe?Ile?Leu?Arg?Arg?Leu?Lys?Thr?Asp?Arg?Asp?Ile?Ile?Gln?Asp?Leu

805?????????????????810?????????????????815

Pro?Asp?Lys?Gln?Glu?Met?Thr?Val?Phe?Cys?Gly?Leu?Thr?Gly?Glu?Gln

820?????????????????825?????????????????830

Ala?Ala?Leu?Tyr?Gln?Lys?Val?Val?Glu?Thr?Ser?Leu?Ala?Glu?Ile?Glu

835?????????????????840?????????????????845

Ser?Ala?Glu?Gly?Leu?Gln?Arg?Arg?Gly?Met?Ile?Leu?Ala?Leu?Leu?Ile

850?????????????????855?????????????????860

Lys?Leu?Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Gln?Tyr?Leu?Lys?Thr?Asn

865?????????????????870?????????????????875?????????????????880

Thr?Leu?Glu?Gln?Tyr?Ser?Ser?Gly?Lys?Leu?Gln?Arg?Leu?Glu?Glu?Met

885?????????????????890?????????????????895

Leu?Glu?Glu?Val?Leu?Ala?Glu?Ser?Asn?Thr?Tyr?Gly?Val?Ala?Gly?Ala

900?????????????????905?????????????????910

Gly?Arg?Ala?Leu?Ile?Phe?Thr?Gln?Phe?Ala?Glu?Trp?Gly?Lys?Leu?Leu

915?????????????????920?????????????????925

Lys?Pro?His?Leu?Glu?Lys?Gln?Leu?Gly?Arg?Glu?Val?Phe?Phe?Leu?Tyr

930?????????????????935?????????????????940

Gly?Ser?Thr?Ser?Lys?Lys?Gln?Arg?Glu?Glu?Met?Ile?Asp?Arg?Phe?Gln

945?????????????????950?????????????????955?????????????????960

His?Asp?Pro?Gln?Gly?Pro?Pro?Ile?Met?Ile?Leu?Ser?Leu?Lys?Ala?Gly

965?????????????????970?????????????????975

Gly?Val?Gly?Leu?Asn?Leu?Thr?Arg?Ala?Asn?His?Val?Phe?His?Phe?Asp

980?????????????????985?????????????????990

Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu??Asn?Gln?Ala?Thr?Asp??Arg?Val?Phe

995?????????????????1000?????????????????1005

Arg?Ile??Gly?Gln?Thr?Arg?Asn??Val?Gln?Val?His?Lys??Phe?Val?Cys

1010?????????????????1015?????????????????1020

Asn?Gly??Thr?Leu?Glu?Glu?Lys??Ile?His?Asp?Met?Ile??Glu?Ser?Lys

1025?????????????????1030?????????????????1035

Lys?Gln??Leu?Ala?Glu?Gln?Val??Val?Gly?Ala?Gly?Glu??Glu?Trp?Leu

1040?????????????????1045?????????????????1050

Thr?Glu??Leu?Asp?Thr?Asp?Gln??Leu?Arg?Asn?Leu?Leu??Ile?Leu?Asp

1055?????????????????1060?????????????????1065

Arg?Ser??Ala?Val?Ile?Asp?Glu??Glu?Ala?Glu

1070?????????????????1075

<210>33

<211>3129

<212>DNA

＜213〉the product methane archaeon that does not cultivate

<400>33

atgattacac?ttcacggaac?ctggactact?gtcgatcccc?tgaatggcac?atttttcctc?????60

tggggagaga?gtgatccggc?cacgcagcat?aaaagaagag?gcaggcctcg?gaaaagtgca????120

ggggagaaac?agcacccgtt?tcacgccggc?atcaaagagc?tggaagctgg?agcgggggct????180

atcaattcat?cgtgtataag?acatatagca?gatgcgggag?cacgggcgga?gcaggtttta????240

attttgccgt?cagctacgga?caggcccctg?agatctgcga?gcccttcagc?actggagtca????300

ggtgaagaaa?ccaaccctga?cagcagttta?caatttcttc?cgtggacggt?gaccggcatc????360

aacattaagc?ccgggaatgc?tctggtactt?ctatcctcta?tagccgaatc?acaaaagcgg????420

atcggagata?tggcgatagg?cccagacctg?ctttactgga?gtaaggtagc?caagtttacg????480

cttaagctcc?tgataagcca?gcagttcagg?ccggaggttg?tcgaagtaat?gagcggaaaa????540

gcatatagcc?gttggagatt?tgcgctcacc?gatgaaactg?accggaaaca?ctatgcctcg????600

ctcgaaaact?ccatgccgct?ggcatgtatt?gcggtttcag?gaaaggctgg?catttataat????660

cgaaaagaag?ccttagattt?gttcattaat?accgcccttg?acacatttat?ccgggaccag????720

attgccctgc?ccgctgacag?caggatgacg?aacctgctat?cgcaagcatg?gctagattcg????780

ctcggcaccg?gagagagtat?ccgcctgtcg?gctcctgaga?tgaagaaact?caaagattcg????840

gcaggccgct?ggacatcccg?catgaaaaca?gagagcaaac?aagctttaaa?gacctgcttc????900

atcctggagc?cgccagcccc?ggatacagag?tatcctgaag?cgccgtggaa?cctacggtac????960

tgcttgcagg?catccgatga?ccccagtctg?gtaattccgg?ctgagactgt?gtggaaagag???1020

ttgaagaaga?cgctgaagta?cctgaataag?agatacgata?accctcagga?gcaattgtta???1080

caggatctcg?gaaaagcgat?gcagatgttt?cccgaaatcg?agcccagcct?caacacgtca???1140

aaacctctgt?ccgcaacgct?gagcaccagt?gaagcctaca?agttcctgac?agaagcggcg???1200

cctctgctgc?aggacagcgg?gtatagcatt?atcctaccgg?aatggtggcg?caacagcact???1260

ggcaggctca?agctcggcgc?caggcttcgc?ttcaagccga?aagccgaagg?taaagcgggt???1320

aaaagccagt?tcaccatgga?taccctcgtc?agctacgact?ggcgcctggc?gctgggcgat???1380

caggagatca?ccgaaacaga?gttcaggaag?ctggcagccc?tgaaagagcc?gcttctgcag???1440

ataggcggga?aatggtttgc?gctgaaaaag?gaagacatag?acagcatcat?gaaagcattc???1500

agggcgaaga?agactggaga?gatggcttta?tcggaggcac?tgcgcctcaa?cggcgggctg???1560

gaagacttca?acggcatccc?cgtcagcggc?atgaaatcgt?caggatggct?ggcagaactt????1620

ttcgacaggc?tggcagccgg?cgaaaaaata?acgagccttg?ccccgccgga?cggtttcaac????1680

ggggagctta?gagattacca?ggttaaaggc?tactcctggc?tggccttcat?gaaaaagtat????1740

ggcctgggct?ccattctggc?tgacgacatg?ggcctgggta?agacgataca?gctgctggcg????1800

ttgctcctga?aagagaagga?aagaggcact?aaaggcccta?ctctgttgat?ctgccccacc????1860

tcgattctcg?gaaactggca?gcgggaggcg?aagaaatttg?ccccggccct?gaaagtccac????1920

atacaccatg?gggcaggaag?ggctgataaa?gagcagttcg?gaaaaatcgt?caaggctcac????1980

gacctgatcc?tgagcactta?cgctcacgcc?taccgggacg?aggaactgct?taaagaggtg????2040

aactggaagc?tggtagtgct?cgacgaggct?cagaatatca?agaatcatca?tacccggcag????2100

gccagagcta?tccgggctct?taaggccgat?caccgaatag?ccatgacggg?aacgccgata????2160

gagaacagac?tctcggagct?gtggtcgatc?gtggacttcc?tgaaccccgg?ctacctgggc????2220

aaggcggaga?cattcaggaa?acaattcgcc?atacctatcg?agagatacga?tgacgctgcc????2280

cggtcggaaa?aattgaagca?ggccatcaag?cccctggtgc?tgcgcagagt?gaagacggat????2340

ccggccatca?tcaaagacct?gccggacaag?atcgagatca?aggagccctg?caacctcacc????2400

aaagaacagg?ccacgctcta?cgaggccatc?gtagagaaca?tgctgaaaag?tatagataag????2460

gccacggcaa?tgcagagacg?gggaatcgtc?ttagcgtccc?tgatgaagct?caaacaggtc????2520

tgcgatcacc?cgtcgctgta?catcaaaacg?ggcgctgtga?ccgacgataa?gacgctgatc????2580

aggtctggca?agctgaagcg?cctcacggag?ctgctcgaag?aagcgctggc?cgaaggcgac????2640

agcgtgctga?tcttcaccca?gttcgtggaa?atgggggaga?tgctgaaagc?ctacctgcag????2700

agcacgttcg?acgaagaagc?cctctttttg?cacggcggag?taccgcagaa?ggccagagac????2760

aagatggtcc?tccgtttcgg?ggaaaaggac?gggccacgga?tctttatcgt?ctcgctgaaa????2820

gccggcggcg?tcggcctcaa?cctgacgaag?gcaagccacg?tgttccactt?cgatcgctgg????2880

tggaacccgg?cggtcgagaa?ccaggcgaca?gatcgagctt?acaggatagg?ccagagcaaa??2940

aatgtactgg?tccataaatt?cgtctgcgcc?ggcacgctgg?aagaaaagat?cgacgagctg??3000

atcgagagca?aaaaggcgct?gtcggcgaac?atcctcggca?cgggagaaga?ctggatcacg??3060

gagttgtcga?ccgaacagct?gagggacatg?gtcatgctga?gatgggacga?ggtagccgat??3120

gatggctaa??????????????????????????????????????????????????????????3129

<210>34

<211>1042

<212>PRT

＜213〉the product methane archaeon that does not cultivate

<400>34

Met?Ile?Thr?Leu?His?Gly?Thr?Trp?Thr?Thr?Val?Asp?Pro?Leu?Asn?Gly

1???????????????5???????????????????10??????????????????15

Thr?Phe?Phe?Leu?Trp?Gly?Glu?Ser?Asp?Pro?Ala?Thr?Gln?His?Lys?Arg

20??????????????????25??????????????????30

Arg?Gly?Arg?Pro?Arg?Lys?Ser?Ala?Gly?Glu?Lys?Gln?His?Pro?Phe?His

35??????????????????40??????????????????45

Ala?Gly?Ile?Lys?Glu?Leu?Glu?Ala?Gly?Ala?Gly?Ala?Ile?Asn?Ser?Ser

50??????????????????55??????????????????60

Cys?Ile?Arg?His?Ile?Ala?Asp?Ala?Gly?Ala?Arg?Ala?Glu?Gln?Val?Leu

65??????????????????70??????????????????75??????????????????80

Ile?Leu?Pro?Ser?Ala?Thr?Asp?Arg?Pro?Leu?Arg?Ser?Ala?Ser?Pro?Ser

85??????????????????90??????????????????95

Ala?Leu?Glu?Ser?Gly?Glu?Glu?Thr?Asn?Pro?Asp?Ser?Ser?Leu?Gln?Phe

100?????????????????105?????????????????110

Leu?Pro?Trp?Thr?Val?Thr?Gly?Ile?Asn?Ile?Lys?Pro?Gly?Asn?Ala?Leu

115?????????????????120?????????????????125

Val?Leu?Leu?Ser?Ser?Ile?Ala?Glu?Ser?Gln?Lys?Arg?Ile?Gly?Asp?Met

130?????????????????135?????????????????140

Ala?Ile?Gly?Pro?Asp?Leu?Leu?Tyr?Trp?Ser?Lys?Val?Ala?Lys?Phe?Thr

145?????????????????150?????????????????155?????????????????160

Leu?Lys?Leu?Leu?Ile?Ser?Gln?Gln?Phe?Arg?Pro?Glu?Val?Val?Glu?Val

165?????????????????170?????????????????175

Met?Ser?Gly?Lys?Ala?Tyr?Ser?Arg?Trp?Arg?Phe?Ala?Leu?Thr?Asp?Glu

180?????????????????185?????????????????190

Thr?Asp?Arg?Lys?His?Tyr?Ala?Ser?Leu?Glu?Asn?Ser?Met?Pro?Leu?Ala

195?????????????????200?????????????????205

Cys?Ile?Ala?Val?Ser?Gly?Lys?Ala?Gly?Ile?Tyr?Asn?Arg?Lys?Glu?Ala

210?????????????????215?????????????????220

Leu?Asp?Leu?Phe?Ile?Asn?Thr?Ala?Leu?Asp?Thr?Phe?Ile?Arg?Asp?Gln

225?????????????????230?????????????????235?????????????????240

Ile?Ala?Leu?Pro?Ala?Asp?Ser?Arg?Met?Thr?Asn?Leu?Leu?Ser?Gln?Ala

245?????????????????250?????????????????255

Trp?Leu?Asp?Ser?Leu?Gly?Thr?Gly?Glu?Ser?Ile?Arg?Leu?Ser?Ala?Pro

260?????????????????265?????????????????270

Glu?Met?Lys?Lys?Leu?Lys?Asp?Ser?Ala?Gly?Arg?Trp?Thr?Ser?Arg?Met

275?????????????????280?????????????????285

Lys?Thr?Glu?Ser?Lys?Gln?Ala?Leu?Lys?Thr?Cys?Phe?Ile?Leu?Glu?Pro

290?????????????????295?????????????????300

Pro?Ala?Pro?Asp?Thr?Glu?Tyr?Pro?Glu?Ala?Pro?Trp?Asn?Leu?Arg?Tyr

305?????????????????310?????????????????315?????????????????320

Cys?Leu?Gln?Ala?Ser?Asp?Asp?Pro?Ser?Leu?Val?Ile?Pro?Ala?Glu?Thr

325?????????????????330?????????????????335

Val?Trp?Lys?Glu?Leu?Lys?Lys?Thr?Leu?Lys?Tyr?Leu?Asn?Lys?Arg?Tyr

340?????????????????345?????????????????350

Asp?Asn?Pro?Gln?Glu?Gln?Leu?Leu?Gln?Asp?Leu?Gly?Lys?Ala?Met?Gln

355?????????????????360?????????????????365

Met?Phe?Pro?Glu?Ile?Glu?Pro?Ser?Leu?Asn?Thr?Ser?Lys?Pro?Leu?Ser

370?????????????????375?????????????????380

Ala?Thr?Leu?Ser?Thr?Ser?Glu?Ala?Tyr?Lys?Phe?Leu?Thr?Glu?Ala?Ala

385?????????????????390?????????????????395?????????????????400

Pro?Leu?Leu?Gln?Asp?Ser?Gly?Tyr?Ser?Ile?Ile?Leu?Pro?Glu?Trp?Trp

405?????????????????410?????????????????415

Arg?Asn?Ser?Thr?Gly?Arg?Leu?Lys?Leu?Gly?Ala?Arg?Leu?Arg?Phe?Lys

420?????????????????425?????????????????430

Pro?Lys?Ala?Glu?Gly?Lys?Ala?Gly?Lys?Ser?Gln?Phe?Thr?Met?Asp?Thr

435?????????????????440?????????????????445

Leu?Val?Ser?Tyr?Asp?Trp?Arg?Leu?Ala?Leu?Gly?Asp?Gln?Glu?Ile?Thr

450?????????????????455?????????????????460

Glu?Thr?Glu?Phe?Arg?Lys?Leu?Ala?Ala?Leu?Lys?Glu?Pro?Leu?Leu?Gln

465?????????????????470?????????????????475?????????????????480

Ile?Gly?Gly?Lys?Trp?Phe?Ala?Leu?Lys?Lys?Glu?Asp?Ile?Asp?Ser?Ile

485?????????????????490?????????????????495

Met?Lys?Ala?Phe?Arg?Ala?Lys?Lys?Thr?Gly?Glu?Met?Ala?Leu?Ser?Glu

500?????????????????505?????????????????510

Ala?Leu?Arg?Leu?Asn?Gly?Gly?Leu?Glu?Asp?Phe?Asn?GlyIle?Pro?Val

515?????????????????520?????????????????525

Ser?Gly?Met?Lys?Ser?Ser?Gly?Trp?Leu?Ala?Glu?Leu?Phe?Asp?Arg?Leu

530?????????????????535?????????????????540

Ala?Ala?Gly?Glu?Lys?Ile?Thr?Ser?Leu?Ala?Pro?Pro?Asp?Gly?Phe?Asn

545?????????????????550?????????????????555?????????????????560

Gly?Glu?Leu?Arg?Asp?Tyr?Gln?Val?Lys?Gly?Tyr?Ser?Trp?Leu?Ala?Phe

565?????????????????570?????????????????575

Met?Lys?Lys?Tyr?Gly?Leu?Gly?Ser?Ile?Leu?Ala?Asp?Asp?Met?Gly?Leu

580?????????????????585?????????????????590

Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala?Leu?Leu?Leu?Lys?Glu?Lys?Glu?Arg

595?????????????????600?????????????????605

Gly?Thr?Lys?Gly?Pro?Thr?Leu?Leu?Ile?Cys?Pro?Thr?Ser?Ile?Leu?Gly

610?????????????????615?????????????????620

Asn?Trp?Gln?Arg?Glu?Ala?Lys?Lys?Phe?Ala?Pro?Ala?Leu?Lys?Val?His

625?????????????????630?????????????????635?????????????????640

Ile?His?His?Gly?Ala?Gly?Arg?Ala?Asp?Lys?Glu?Gln?Phe?Gly?Lys?Ile

645?????????????????650?????????????????655

Val?Lys?Ala?His?Asp?Leu?Ile?Leu?Ser?Thr?Tyr?Ala?His?Ala?Tyr?Arg

660?????????????????665?????????????????670

Asp?Glu?Glu?Leu?Leu?Lys?Glu?Val?Asn?Trp?Lys?Leu?Val?Val?Leu?Asp

675?????????????????680?????????????????685

Glu?Ala?Gln?Asn?Ile?Lys?Asn?His?His?Thr?Arg?Gln?Ala?Arg?Ala?Ile

690?????????????????695?????????????????700

Arg?Ala?Leu?Lys?Ala?Asp?His?Arg?Ile?Ala?Met?Thr?Gly?Thr?Pro?Ile

705?????????????????710?????????????????715?????????????????720

Glu?Asn?Arg?Leu?Ser?Glu?Leu?Trp?Ser?Ile?Val?Asp?Phe?Leu?Asn?Pro

725?????????????????730?????????????????735

Gly?Tyr?Leu?Gly?Lys?Ala?Glu?Thr?Phe?Arg?Lys?Gln?Phe?Ala?Ile?Pro

740?????????????????745?????????????????750

Ile?Glu?Arg?Tyr?Asp?Asp?Ala?Ala?Arg?Ser?Glu?Lys?Leu?Lys?Gln?Ala

755?????????????????760?????????????????765

Ile?Lys?Pro?Leu?Val?Leu?Arg?Arg?Val?Lys?Thr?Asp?Pro?Ala?Ile?Ile

770?????????????????775?????????????????780

Lys?Asp?Leu?Pro?Asp?Lys?Ile?Glu?Ile?Lys?Glu?Pro?Cys?Asn?Leu?Thr

785?????????????????790?????????????????795?????????????????800

Lys?Glu?Gln?Ala?Thr?Leu?Tyr?Glu?Ala?Ile?Val?Glu?Asn?Met?Leu?Lys

805?????????????????810?????????????????815

Ser?Ile?Asp?Lys?Ala?Thr?Ala?Met?Gln?Arg?Arg?Gly?Ile?Val?Leu?Ala

820?????????????????825?????????????????830

Ser?Leu?Met?Lys?Leu?Lys?Gln?Val?Cys?Asp?His?Pro?Ser?Leu?Tyr?Ile

835?????????????????840?????????????????845

Lys?Thr?Gly?Ala?Val?Thr?Asp?Asp?Lys?Thr?Leu?Ile?Arg?Ser?Gly?Lys

850?????????????????855?????????????????860

Leu?Lys?Arg?Leu?Thr?Glu?Leu?Leu?Glu?Glu?Ala?Leu?Ala?Glu?Gly?Asp

865?????????????????870?????????????????875?????????????????880

Ser?Val?Leu?Ile?Phe?Thr?Gln?Phe?Val?Glu?Met?Gly?Glu?Met?Leu?Lys

885?????????????????890?????????????????895

Ala?Tyr?Leu?Gln?Ser?Thr?Phe?Asp?Glu?Glu?Ala?Leu?Phe?Leu?His?Gly

900?????????????????905?????????????????910

Gly?Val?Pro?Gln?Lys?Ala?Arg?Asp?Lys?Met?Val?Leu?Arg?Phe?Gly?Glu

915?????????????????920?????????????????925

Lys?Asp?Gly?Pro?Arg?Ile?Phe?Ile?Val?Ser?Leu?Lys?Ala?Gly?Gly?Val

930?????????????????935?????????????????940

Gly?Leu?Asn?Leu?Thr?Lys?Ala?Ser?His?Val?Phe?His?Phe?Asp?Arg?Trp

945?????????????????950?????????????????955?????????????????960

Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp?Arg?Ala?Tyr?Arg?Ile

965?????????????????970?????????????????975

Gly?Gln?Ser?Lys?Asn?Val?Leu?Val?His?Lys?Phe?Val?Cys?Ala?Gly?Thr

980?????????????????985?????????????????990

Leu?Glu?Glu?Lys?Ile?Asp?Glu?Leu??Ile?Glu?Ser?Lys?Lys??Ala?Leu?Ser

995?????????????????1000?????????????????1005

Ala?Asn??Ile?Leu?Gly?Thr?Gly??Glu?Asp?Trp?Ile?Thr??Glu?Leu?Ser

1010?????????????????1015?????????????????1020

Thr?Glu??Gln?Leu?Arg?Asp?Met??Val?Met?Leu?Arg?Trp??Asp?Glu?Val

1025?????????????????1030?????????????????1035

Ala?Asp??Asp?Gly

1040

<210>35

<211>2757

<212>DNA

＜213〉bacillus cereus

<400>35

atgatcaatc?aaactgaagt?aacaattagg?ctccagcacg?ttagtcacgg?ttggttcctt???60

tggggagaag?atgatagcgg?tactccatta?tccgtaacaa?gttggaaacg?aaatgcattt??120

acatggcact?ccacttcctt?ctacggcacg?tttctaaaag?aagcaagctt?tgaaggaaga??180

caaggtgtta?tgctaacaaa?cgcacaagca?tttgaataca?tcgcgaataa?accgatgaac??240

tcctttgccc?gtattcaaat?gaacggccct?attacagcac?ttacggaaga?tgcgaacgaa??300

ttgtgggatg?ccttcacaag?cggtagcttc?gtacctgata?tggagcgttg?gcctaaacaa??360

ccatcttgga?aagttcaaaa?tactccaatc?gaagatgaaa?cattggcatc?tcttttctcg??420

gctgcagtaa?atgaaagcat?attacaagat?aaccgttcaa?atgacggatg?ggaagatgca??480

aagagacttt?atgaacatta?cgactttacg?aaaagacaat?tagacgcagc?actacatgaa??540

gaagattggc?ttcgaaaaat?tggttacatt?gaagatgacc?ttccctttac?aatcggacta??600

cgactacaag?agccgcaaga?agaatttgaa?atgtggaagc?ttgaaacaat?tgttacgcca?????660

aagcgcgggg?cacatcgcat?atatgtatat?gagagtatcg?attctttacc?aaaacgatgg?????720

cacgattatg?aagaacgtat?tctggaaaca?caagaaagct?tcagtaagct?cgtaccgtgg?????780

ctaaaagatg?gtgatacatt?ccgaagtgaa?ctctttgaaa?cagaagcgtg?gaacttctta?????840

acagaagcaa?gtaacgaatt?actcgccgca?ggtattacaa?tcttattacc?atcgtggtgg?????900

caaaatttaa?aagcgacaaa?accaaaatta?cgtgtgcaac?tgaagcaaaa?tgctacacaa?????960

acgcaatctt?tcttcggcat?gaatacactc?gttaattttg?actggcgcat?ttcaacgaac????1020

ggcattgatt?tatcagaaag?cgaatttttt?gaactcgttg?aacaaaacaa?gcggttattc????1080

aatataaatg?gtcaatggat?gcgactagat?ccagccttta?ttgaagaagt?acgaaagctc????1140

atgaatcgtg?ctgataagta?tggacttgaa?atgaaagatg?tcctgcagca?acatttatca????1200

aacacggctg?aaacagaaat?tgtagaagag?gatagtccgt?ttacagatat?tgaaattgaa????1260

ctagatggat?attatgaaga?cttattccaa?aaactattgc?acattggaga?tattccgaaa????1320

gtagatgtcc?cttcatcact?aaacgccaca?ctccgtccgt?atcaacaaca?tggcattgag????1380

tggttattat?atttaagaaa?gcttggattc?ggcgcattgt?tagctgacga?catgggactt????1440

ggaaagagta?ttcaaacgat?cacttactta?ctatatataa?aagaaaacaa?tctccaaaca????1500

ggtcctgctt?taatcgtggc?tccgacatct?gttcttggaa?attggcaaaa?agaatttgag????1560

cgtttcgcac?cgaatttacg?tgttcagtta?cattatggaa?gtaaccgagc?taaaggggaa????1620

ccctttaaag?atttccttca?atcagcagat?gttgtattaa?catcttatgc?attagctcag????1680

cttgatgagg?aagaacttag?tacgttatgc?tgggatgctg?ttattttgga?tgaagcacaa????1740

aatattaaaa?acccacatac?gaaacagtct?aaagcagtac?gaaacttaca?agcaaatcac????1800

aaaatcgcat?taactgggac?accgatggaa?aaccgccttg?ccgagctttg?gtctattttc????1860

gacttcatta?atcatggata?tcttggcagc?ttaggacaat?tccagcgccg?cttcgtctca????1920

ccaattgaaa?aggaccgtga?cgaaggaaaa?atccaacaag?ttcaacgttt?tatctcaccg??1980

tttttactgc?gtcgtacgaa?gaaagatcaa?acagtcgcat?taaacttacc?agataaacaa??2040

gaacagaaag?cttactgtcc?actaactggt?gaacaagctt?ccttatatga?acaacttgtt??2100

caagatacgt?tgcaaaatgt?agaaggatta?agcggaattg?aacgacgcgg?atttatatta??2160

ctcatgctga?acaaacttaa?acaaatttgt?aatcatcccg?ctctttattt?aaaagaaaca??2220

gaaccgaaag?acatcatcga?gcgttccatg?aaaacgagca?cgctcatgga?actcattgaa??2280

aatataaaag?atcaaaatga?aagttgctta?atcttcacgc?aatacatcgg?tatggggaac??2340

atgctaaaag?atgtgttaga?agaacatttc?ggtcagcgcg?tcctcttctt?aaacggtagt??2400

gtaccgaaga?aagaacgtga?caaaatgatc?gaacagttcc?aaaacggaac?gtatgacatc??2460

ttcattttat?cgttaaaagc?aggtggtaca?ggattaaact?taacagctgc?caaccatgtc??2520

attcactacg?atcgttggtg?gaatccagcg?gtagaaaacc?aagcaacaga?ccgtgcatat??2580

cgcattggtc?aaaagcgctt?cgttcacgtt?cataaactga?ttacaacggg?gacacttgaa??2640

gagaaaatcg?atgaaatgtt?agaaagaaaa?caatcattaa?acaacgccgt?cattacaagc??2700

gatagttgga?tgacagaact?atctacagat?gaactaaaag?aattacttgg?tgtataa?????2757

<210>36

<211>918

<212>PRT

＜213〉bacillus cereus

<400>36

Met?Ile?Asn?Gln?Thr?Glu?Val?Thr?Ile?Arg?Leu?Gln?His?Val?Ser?His

1???????????????5???????????????????10??????????????????15

Gly?Trp?Phe?Leu?Trp?Gly?Glu?Asp?Asp?Ser?Gly?Thr?Pro?Leu?Ser?Val

20??????????????????25??????????????????30

Thr?Ser?Trp?Lys?Arg?Asn?Ala?Phe?Thr?Trp?His?Ser?Thr?Ser?Phe?Tyr

35??????????????????40??????????????????45

Gly?Thr?Phe?Leu?Lys?Glu?Ala?Ser?Phe?Glu?Gly?Arg?Gln?Gly?Val?Met

50??????????????????55??????????????????60

Leu?Thr?Asn?Ala?Gln?Ala?Phe?Glu?Tyr?Ile?Ala?Asn?Lys?Pro?Met?Asn

65??????????????????70??????????????????75??????????????????80

Ser?Phe?Ala?Arg?Ile?Gln?Met?Asn?Gly?Pro?Ile?Thr?Ala?Leu?Thr?Glu

85??????????????????90??????????????????95

Asp?Ala?Asn?Glu?Leu?Trp?Asp?Ala?Phe?Thr?Ser?Gly?Ser?Phe?Val?Pro

100?????????????????105?????????????????110

Asp?Met?Glu?Arg?Trp?Pro?Lys?Gln?Pro?Ser?Trp?Lys?Val?Gln?Asn?Thr

115?????????????????120?????????????????125

Pro?Ile?Glu?Asp?Glu?Thr?Leu?Ala?Ser?Leu?Phe?Ser?Ala?Ala?Val?Asn

130?????????????????135?????????????????140

Glu?Ser?Ile?Leu?Gln?Asp?Asn?Arg?Ser?Asn?Asp?Gly?Trp?Glu?Asp?Ala

145?????????????????150?????????????????155?????????????????160

Lys?Arg?Leu?Tyr?Glu?His?Tyr?Asp?Phe?Thr?Lys?Arg?Gln?Leu?Asp?Ala

165?????????????????170?????????????????175

Ala?Leu?His?Glu?Glu?Asp?Trp?Leu?Arg?Lys?Ile?Gly?Tyr?Ile?Glu?Asp

180?????????????????185?????????????????190

Asp?Leu?Pro?Phe?Thr?Ile?Gly?Leu?Arg?Leu?Gln?Glu?Pro?Gln?Glu?Glu

195?????????????????200?????????????????205

Phe?Glu?Met?Trp?Lys?Leu?Glu?Thr?Ile?Val?Thr?Pro?Lys?Arg?Gly?Ala

210?????????????????215?????????????????220

His?Arg?Ile?Tyr?Val?Tyr?Glu?Ser?Ile?Asp?Ser?Leu?Pro?Lys?Arg?Trp

225?????????????????230?????????????????235?????????????????240

His?Asp?Tyr?Glu?Glu?Arg?Ile?Leu?Glu?Thr?Gln?Glu?Ser?Phe?Ser?Lys

245?????????????????250?????????????????255

Leu?Val?Pro?Trp?Leu?Lys?Asp?Gly?Asp?Thr?Phe?Arg?Ser?Glu?Leu?Phe

260?????????????????265?????????????????270

Glu?Thr?Glu?Ala?Trp?Asn?Phe?Leu?Thr?Glu?Ala?Ser?Asn?Glu?Leu?Leu

275?????????????????280?????????????????285

Ala?Ala?Gly?Ile?Thr?Ile?Leu?Leu?Pro?Ser?Trp?Trp?Gln?Asn?Leu?Lys

290?????????????????295?????????????????300

Ala?Thr?Lys?Pro?Lys?Leu?Arg?Val?Gln?Leu?Lys?Gln?Asn?Ala?Thr?Gln

305?????????????????310?????????????????315?????????????????320

Thr?Gln?Ser?Phe?Phe?Gly?Met?Asn?Thr?Leu?Val?Asn?Phe?Asp?Trp?Arg

325?????????????????330?????????????????335

Ile?Ser?Thr?Asn?Gly?Ile?Asp?Leu?Ser?Glu?Ser?Glu?Phe?Phe?Glu?Leu

340?????????????????345?????????????????350

Val?Glu?Gln?Asn?Lys?Arg?Leu?Phe?Asn?Ile?Asn?Gly?Gln?Trp?Met?Arg

355?????????????????360?????????????????365

Leu?Asp?Pro?Ala?Phe?Ile?Glu?Glu?Val?Arg?Lys?Leu?Met?Asn?Arg?Ala

370?????????????????375?????????????????380

Asp?Lys?Tyr?Gly?Leu?Glu?Met?Lys?Asp?Val?Leu?Gln?Gln?His?Leu?Ser

385?????????????????390?????????????????395?????????????????400

Asn?Thr?Ala?Glu?Thr?Glu?Ile?Val?Glu?Glu?Asp?Ser?Pro?Phe?Thr?Asp

405?????????????????410?????????????????415

Ile?Glu?Ile?Glu?Leu?Asp?Gly?Tyr?Tyr?Glu?Asp?Leu?Phe?Gln?Lys?Leu

420?????????????????425?????????????????430

Leu?His?Ile?Gly?Asp?Ile?Pro?Lys?Val?Asp?Val?Pro?Ser?Ser?Leu?Asn

435?????????????????440?????????????????445

Ala?Thr?Leu?Arg?Pro?Tyr?Gln?Gln?His?Gly?Ile?Glu?Trp?Leu?Leu?Tyr

450?????????????????455?????????????????460

Leu?Arg?Lys?Leu?Gly?Phe?Gly?Ala?Leu?Leu?Ala?Asp?Asp?Met?Gly?Leu

465?????????????????470?????????????????475?????????????????480

Gly?Lys?Ser?Ile?Gln?Thr?Ile?Thr?Tyr?Leu?Leu?Tyr?Ile?Lys?Glu?Asn

485?????????????????490?????????????????495

Asn?Leu?Gln?Thr?Gly?Pro?Ala?Leu?Ile?Val?Ala?Pro?Thr?Ser?Val?Leu

500?????????????????505?????????????????510

Gly?Asn?Trp?Gln?Lys?Glu?Phe?Glu?Arg?Phe?Ala?Pro?Asn?Leu?Arg?Val

515?????????????????520?????????????????525

Gln?Leu?His?Tyr?Gly?Ser?Asn?Arg?Ala?Lys?Gly?Glu?Pro?Phe?Lys?Asp

530?????????????????535?????????????????540

Phe?Leu?Gln?Ser?Ala?Asp?Val?Val?Leu?Thr?Ser?Tyr?Ala?Leu?Ala?Gln

545?????????????????550?????????????????555?????????????????560

Leu?Asp?Glu?Glu?Glu?Leu?Ser?Thr?Leu?Cys?Trp?Asp?Ala?Val?Ile?Leu

565?????????????????570?????????????????575

Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn?Pro?His?Thr?Lys?Gln?Ser?Lys?Ala

580?????????????????585?????????????????590

Val?Arg?Asn?Leu?Gln?Ala?Asn?His?Lys?Ile?Ala?Leu?Thr?Gly?Thr?Pro

595?????????????????600?????????????????605

Met?Glu?Asn?Arg?Leu?Ala?Glu?Leu?Trp?Ser?Ile?Phe?Asp?Phe?Ile?Asn

610?????????????????615?????????????????620

His?Gly?Tyr?Leu?Gly?Ser?Leu?Gly?Gln?Phe?Gln?Arg?Arg?Phe?Val?Ser

625?????????????????630?????????????????635?????????????????640

Pro?Ile?Glu?Lys?Asp?Arg?Asp?Glu?Gly?Lys?Ile?Gln?Gln?Val?Gln?Arg

645?????????????????650?????????????????655

Phe?Ile?Ser?Pro?Phe?Leu?Leu?Arg?Arg?Thr?Lys?Lys?Asp?Gln?Thr?Val

660?????????????????665?????????????????670

Ala?Leu?Asn?Leu?Pro?Asp?Lys?Gln?Glu?Gln?Lys?Ala?Tyr?Cys?Pro?Leu

675?????????????????680?????????????????685

Thr?Gly?Glu?Gln?Ala?Ser?Leu?Tyr?Glu?Gln?Leu?Val?Gln?Asp?Thr?Leu

690?????????????????695?????????????????700

Gln?Asn?Val?Glu?Gly?Leu?Ser?Gly?Ile?Glu?Arg?Arg?Gly?Phe?Ile?Leu

705?????????????????710?????????????????715?????????????????720

Leu?Met?Leu?Asn?Lys?Leu?Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Leu?Tyr

725?????????????????730?????????????????735

Leu?Lys?Glu?Thr?Glu?Pro?Lys?Asp?Ile?Ile?Glu?Arg?Ser?Met?Lys?Thr

740?????????????????745?????????????????750

Ser?Thr?Leu?Met?Glu?Leu?Ile?Glu?Asn?Ile?Lys?Asp?Gln?Asn?Glu?Ser

755?????????????????760?????????????????765

Cys?Leu?Ile?Phe?Thr?Gln?Tyr?Ile?Gly?Met?Gly?Asn?Met?Leu?Lys?Asp

770?????????????????775?????????????????780

Val?Leu?Glu?Glu?His?Phe?Gly?Gln?Arg?Val?Leu?Phe?Leu?Asn?Gly?Ser

785?????????????????790?????????????????795?????????????????800

Val?Pro?Lys?Lys?Glu?Arg?Asp?Lys?Met?Ile?Glu?Gln?Phe?Gln?Asn?Gly

805?????????????????810?????????????????815

Thr?Tyr?Asp?Ile?Phe?Ile?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Thr?Gly?Leu

820?????????????????825?????????????????830

Asn?Leu?Thr?Ala?Ala?Asn?His?Val?Ile?His?Tyr?Asp?Arg?Trp?Trp?Asn

835?????????????????840?????????????????845

Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp?Arg?Ala?Tyr?Arg?Ile?Gly?Gln

850?????????????????855?????????????????860

Lys?Arg?Phe?Val?His?Val?His?Lys?Leu?Ile?Thr?Thr?Gly?Thr?Leu?Glu

865?????????????????870?????????????????875?????????????????880

Glu?Lys?Ile?Asp?Glu?Met?Leu?Glu?Arg?Lys?Gln?Ser?Leu?Asn?Asn?Ala

885?????????????????890?????????????????895

Val?Ile?Thr?Ser?Asp?Ser?Trp?Met?Thr?Glu?Leu?Ser?Thr?Asp?Glu?Leu

900?????????????????905?????????????????910

Lys?Glu?Leu?Leu?Gly?Val

915

<210>37

<211>3141

<212>DNA

<213>Crocosphaera?watsonii

<400>37

atgacaatat?tacatggaac?ttggattgaa?aatacctctg?aaaaacattt?ttttatttgg?????60

ggggaaactt?ggcgttcttt?atcctctgat?atttcctcag?atgattctat?tttaatgtat????120

ccattttctg?tagataaaca?gggaattatt?gaacaattaa?actcgaataa?gattaagatt????180

gaaaaaaaca?aaaatattga?atctgtttct?caaatatttt?atttgcctag?taaatttatt????240

gctaaatcga?agcaaagtat?ccctttacta?tcaacagaat?taaaagataa?agattttgaa????300

caaggggata?ttcagttaat?tgcttggaaa?atcgaaggga?taaaattaaa?tgttgatgat????360

acaattaata?ttttaagtca?gttaccgttg?ggattaacca?ataatgacga?aaattacata????420

ggcgataatt?taaaattttg?gacacatatt?tatcgttgga?gtctagattt?attaactaga????480

ggtaaatatt?taccgcaaat?ggaagaacaa?gataataact?gttatggaca?atgggaacct????540

ttactagata?gtttagttga?tcagcaacgg?ttctctaaat?ttatacaaac?tatgccaaat????600

agttctcttg?cttatcataa?tttaatggag?ggtgaattat?cctcttcttt?actcaaacaa????660

actactattc?ttgatttttt?atctactatc?attaatcaac?aagtacgtca?atttattgat????720

gttgctatta?cccctagttc?atttatccaa?aagtggttat?actctttaac?acaagactta????780

tctaaatttg?aagcatcaga?agttgaaaga?aagggattaa?agaatgctat?taataattgg????840

aaatcttctt?taagtgaata?tattataaag?tctgataatc?aaccattagg?aattaaccag????900

tttcgtgttt?gttttaaact?agaaaatcca?gctaaaagtg?gtaagaaatt?agaacaaagt????960

aattggcagt?tacactacta?tctccaagct?ttagatgatc?ctaattttct?gatctctgcc???1020

aaggttattt?gggaaaatcc?tgttactaga?ttaatctgca?ataatagaac?aattaatcat????1080

cctcaagaaa?ccttgctaaa?aggactaggt?ttagcttcac?gtctatatta?tctaattgaa????1140

gaaagtttac?aagacaataa?gcctagtttt?tctgagttag?atcccataca?agtctatgaa????1200

tttttacgtt?caattgctaa?tattcttaaa?gataatggct?taggggttat?cttaccagct????1260

agtctagagc?aaggagtcga?agaaaaacgc?ttaggaatta?gtctaaccgc?agaagttaag????1320

tcgaaaaaag?gacaaagact?tagcttacaa?agtttgttaa?gttataagct?aaatttagca????1380

attggtgata?aaacaatatc?gaaaaaagac?tttgaaaaac?tattagcgca?aaagtcacct????1440

ttagttgaag?taaaaggaga?atggatagca?ttacaacctg?ctgatgtcaa?ggccgcacaa????1500

caaattttaa?ataagtccta?tgatccccta?gaactttctg?tagaagatgc?tttacgcttc????1560

agcacaggag?atatttcaac?tgttgccaaa?ctgccgatta?ctaactttga?agcaaaaggg????1620

gaattagcca?atctaattaa?tgctataaat?aataatgaat?caatccctat?gatcgaaaat????1680

cccagaggat?ttaaaggtca?attacgtccc?tatcaacagc?gaggagtcgg?ttggttatcg????1740

ttcttagaaa?aatggggttt?aggggcttgt?cttgccgatg?atatgggatt?aggaaaaaca????1800

ccacaattaa?ttgggtttct?cttacattta?agaagcgaag?gaatgttaga?tcaacctacc????1860

ttagttattt?gtcctacatc?tgttttaaat?aactgggaaa?gagaagttca?aaaatttgcc????1920

ccaacccttt?ctactttgat?tcatcatgga?gataaacgta?gtaaagggaa?agcttttgtt????1980

aaagcagtta?gtaaaaaaaa?tgttatcatt?actagctatt?ctttaattta?tcgagatatt????2040

aaaagctttg?aacaggtaga?atggcaaggt?attgtcttag?atgaagcaca?aaatataaaa????2100

aatccccagg?caaaacaatc?ccaagcagtg?cgtcaaattt?ccacacagtt?tcgtattgct????2160

ttaacaggaa?ctcctgtaga?aaatcgccta?acagaattat?ggtcaattct?tgactttctt????2220

aacccaggat?ttttagggac?acagcagttt?ttccgtcgtc?gttttgccac?tcctatcgaa????2280

aaatatgggg?ataaagaatc?actgcaaatt?atgcgttctt?tggtacgtcc?tttcattctc????2340

agacgattga?aaacagataa?aactattatt?caagatttac?ccgaaaaaca?agaaatgacc????2400

attttttgtg?ggttatcctc?agaacaagga?aaactttatc?aacaattagt?agataattct????2460

ctggtagcaa?tagaagagaa?aacaggaatt?gaacgcaaag?gcttaatttt?aagcttactg????2520

ctaaaactca?aacaaatttg?taaccatcct?gctcattttc?tcaagcaaaa?gagcttaaaa????2580

acagcagaac?aatctggtaa?attattaaga?ctagaagaaa?tgctagaaga?attaatcgaa????2640

gaaggagatc?atgctttaat?ctttacccaa?ttttctgaat?ggggtaaact?gctgcaacct????2700

tatttacaga?aaaaatttca?gcaagacgtt?ctctttttgt?atggtgctac?tcgcagagtt????2760

caaagacaag?aaatgatcga?tcgctttcaa?caggatccca?acggacccag?aatttttatt????2820

ctctccttaa?aagcaggggg?aaccggatta?aatttaaccc?gcgctaacca?tgtatttcat????2880

attgatcgtt?ggtggaaccc?agcagtagaa?aatcaagcaa?ccgatcgcgc?gtttcgttta????2940

ggacaaaaac?gcaatgttca?agtacataaa?tttgtctgta?caggaaccct?agaagaaaaa????3000

attaacgaaa?tgttagaaag?taaacaaaaa?ttagccgaac?aaaccgttga?cgcaggggaa????3060

caatggttga?cagaattaga?tacagatcaa?ctgcgtaacc?tcttattatt?ggatcgagat????3120

accattattg?acgaacaata?a??????????????????????????????????????????????3141

<210>38

<211>1046

<212>PRT

<213>Crocosphaera?watsonii

<400>38

Met?Thr?Ile?Leu?His?Gly?Thr?Trp?Ile?Glu?Asn?Thr?Ser?Glu?Lys?His

1??????????????5????????????????????10??????????????????15

Phe?Phe?Ile?Trp?Gly?Glu?Thr?Trp?Arg?Ser?Leu?Ser?Ser?Asp?Ile?Ser

20??????????????????25??????????????????30

Ser?Asp?Asp?Ser?Ile?Leu?Met?Tyr?Pro?Phe?Ser?Val?Asp?Lys?Gln?Gly

35??????????????????40??????????????????45

Ile?Ile?Glu?Gln?Leu?Asn?Ser?Asn?Lys?Ile?Lys?Ile?Glu?Lys?Asn?Lys

50??????????????????55??????????????????60

Asn?Ile?Glu?Ser?Val?Ser?Gln?Ile?Phe?Tyr?Leu?Pro?Ser?Lys?Phe?Ile

65??????????????????70??????????????????75??????????????????80

Ala?Lys?Ser?Lys?Gln?Ser?Ile?Pro?Leu?Leu?Ser?Thr?Glu?Leu?Lys?Asp

85??????????????????90??????????????????95

Lys?Asp?Phe?Glu?Gln?Gly?Asp?Ile?Gln?Leu?Ile?Ala?Trp?Lys?Ile?Glu

100?????????????????105?????????????????110

Gly?Ile?Lys?Leu?Asn?Val?Asp?Asp?Thr?Ile?Asn?Ile?Leu?Ser?Gln?Leu

115?????????????????120?????????????????125

Pro?Leu?Gly?Leu?Thr?Asn?Asn?Asp?Glu?Asn?Tyr?Ile?Gly?Asp?Asn?Leu

130?????????????????135?????????????????140

Lys?Phe?Trp?Thr?His?Ile?Tyr?Arg?Trp?Ser?Leu?Asp?Leu?Leu?Thr?Arg

145?????????????????150?????????????????155?????????????????160

Gly?Lys?Tyr?Leu?Pro?Gln?Met?Glu?Glu?Gln?Asp?Asn?Asn?Cys?Tyr?Gly

165?????????????????170?????????????????175

Gln?Trp?Glu?Pro?Leu?Leu?Asp?Ser?Leu?Val?Asp?Gln?Gln?Arg?Phe?Ser

180?????????????????185?????????????????190

Lys?Phe?Ile?Gln?Thr?Met?Pro?Asn?Ser?Ser?Leu?Ala?Tyr?His?Asn?Leu

195?????????????????200?????????????????205

Met?Glu?Gly?Glu?Leu?Ser?Ser?Ser?Leu?Leu?Lys?Gln?Thr?Thr?Ile?Leu

210?????????????????215?????????????????220

Asp?Phe?Leu?Ser?Thr?Ile?Ile?Asn?Gln?Gln?Val?Arg?Gln?Phe?Ile?Asp

225?????????????????230?????????????????235?????????????????240

Val?Ala?Ile?Thr?Pro?Ser?Ser?Phe?Ile?Gln?Lys?Trp?Leu?Tyr?Ser?Leu

245?????????????????250?????????????????255

Thr?Gln?Asp?Leu?Ser?Lys?Phe?Glu?Ala?Ser?Glu?Val?Glu?Arg?Lys?Gly

260?????????????????265?????????????????270

Leu?Lys?Asn?Ala?Ile?Asn?Asn?Trp?Lys?Ser?Ser?Leu?Ser?Glu?Tyr?Ile

275?????????????????280?????????????????285

Ile?Lys?Ser?Asp?Asn?Gln?Pro?Leu?Gly?Ile?Asn?Gln?Phe?Arg?Val?Cys

290?????????????????295?????????????????300

Phe?Lys?Leu?Glu?Asn?Pro?Ala?Lys?Ser?Gly?Lys?Lys?Leu?Glu?Gln?Ser

305?????????????????310?????????????????315?????????????????320

Asn?Trp?Gln?Leu?His?Tyr?Tyr?Leu?Gln?Ala?Leu?Asp?Asp?Pro?Asn?Phe

325?????????????????330?????????????????335

Leu?Ile?Ser?Ala?Lys?Val?Ile?Trp?Glu?Asn?Pro?Val?Thr?Arg?Leu?Ile

340?????????????????345?????????????????350

Cys?Asn?Asn?Arg?Thr?Ile?Asn?His?Pro?Gln?Glu?Thr?Leu?Leu?Lys?Gly

355?????????????????360?????????????????365

Leu?Gly?Leu?Ala?Ser?Arg?Leu?Tyr?Tyr?Leu?Ile?Glu?Glu?Ser?Leu?Gln

370?????????????????375?????????????????380

Asp?Asn?Lys?Pro?Ser?Phe?Ser?Glu?Leu?Asp?Pro?Ile?Gln?Val?Tyr?Glu

385?????????????????390?????????????????395?????????????????400

Phe?Leu?Arg?Ser?Ile?Ala?Asn?Ile?Leu?Lys?Asp?Asn?Gly?Leu?Gly?Val

405?????????????????410?????????????????415

Ile?Leu?Pro?Ala?Ser?Leu?Glu?Gln?Gly?Val?Glu?Glu?Lys?Arg?Leu?Gly

420?????????????????425?????????????????430

Ile?Ser?Leu?Thr?Ala?Glu?Val?Lys?Ser?Lys?Lys?Gly?Gln?Arg?Leu?Ser

435?????????????????440?????????????????445

Leu?Gln?Ser?Leu?Leu?Ser?Tyr?Lys?Leu?Asn?Leu?Ala?Ile?Gly?Asp?Lys

450?????????????????455?????????????????460

Thr?Ile?Ser?Lys?Lys?Asp?Phe?Glu?Lys?Leu?Leu?Ala?Gln?Lys?Ser?Pro

465?????????????????470?????????????????475?????????????????480

Leu?Val?Glu?Val?Lys?Gly?Glu?Trp?Ile?Ala?Leu?Gln?Pro?Ala?Asp?Val

485?????????????????490?????????????????495

Lys?Ala?Ala?Gln?Gln?Ile?Leu?Asn?Lys?Ser?Tyr?Asp?Pro?Leu?Glu?Leu

500?????????????????505?????????????????510

Ser?Val?Glu?Asp?Ala?Leu?Arg?Phe?Ser?Thr?Gly?Asp?Ile?Ser?Thr?Val

515?????????????????520?????????????????525

Ala?Lys?Leu?Pro?Ile?Thr?Asn?Phe?Glu?Ala?Lys?Gly?Glu?Leu?Ala?Asn

530?????????????????535?????????????????540

Leu?Ile?Asn?Ala?Ile?Asn?Asn?Asn?Glu?Ser?Ile?Pro?Met?Ile?Glu?Asn

545?????????????????550?????????????????555?????????????????560

Pro?Arg?Gly?Phe?Lys?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Gln?Arg?Gly?Val

565?????????????????570?????????????????575

Gly?Trp?Leu?Ser?Phe?Leu?Glu?Lys?Trp?Gly?Leu?Gly?Ala?Cys?Leu?Ala

580?????????????????585?????????????????590

Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Pro?Gln?Leu?Ile?Gly?Phe?Leu?Leu

595?????????????????600?????????????????605

His?Leu?Arg?Ser?Glu?Gly?Met?Leu?Asp?Gln?Pro?Thr?Leu?Val?Ile?Cys

610?????????????????615?????????????????620

Pro?Thr?Ser?Val?Leu?Asn?Asn?Trp?Glu?Arg?Glu?Val?Gln?Lys?Phe?Ala

625?????????????????630?????????????????635?????????????????640

Pro?Thr?Leu?Ser?Thr?Leu?Ile?His?His?Gly?Asp?Lys?Arg?Ser?Lys?Gly

645?????????????????650?????????????????655

Lys?Ala?Phe?Val?Lys?Ala?Val?Ser?Lys?Lys?Asn?Val?Ile?Ile?Thr?Ser

660?????????????????665?????????????????670

Tyr?Ser?Leu?Ile?Tyr?Arg?Asp?Ile?Lys?Ser?Phe?Glu?Gln?Val?Glu?Trp

675?????????????????680?????????????????685

Gln?Gly?Ile?Val?Leu?Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn?Pro?Gln?Ala

690?????????????????695?????????????????700

Lys?Gln?Ser?Gln?Ala?Val?Arg?Gln?Ile?Ser?Thr?Gln?Phe?Arg?Ile?Ala

705?????????????????710?????????????????715?????????????????720

Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu?Thr?Glu?Leu?Trp?Ser?Ile

725?????????????????730?????????????????735

Leu?Asp?Phe?Leu?Asn?Pro?Gly?Phe?Leu?Gly?Thr?Gln?Gln?Phe?Phe?Arg

740?????????????????745?????????????????750

Arg?Arg?Phe?Ala?Thr?Pro?Ile?Glu?Lys?Tyr?Gly?Asp?Lys?Glu?Ser?Leu

755?????????????????760?????????????????765

Gln?Ile?Met?Arg?Ser?Leu?Val?Arg?Pro?Phe?Ile?Leu?Arg?Arg?Leu?Lys

770?????????????????775?????????????????780

Thr?Asp?Lys?Thr?Ile?Ile?Gln?Asp?Leu?Pro?Glu?Lys?Gln?Glu?Met?Thr

785?????????????????790?????????????????795?????????????????800

Ile?Phe?Cys?Gly?Leu?Ser?Ser?Glu?Gln?Gly?Lys?Leu?Tyr?Gln?Gln?Leu

805?????????????????810?????????????????815

Val?Asp?Asn?Ser?Leu?Val?Ala?Ile?Glu?Glu?Lys?Thr?Gly?Ile?Glu?Arg

820?????????????????825?????????????????830

Lys?Gly?Leu?Ile?Leu?Ser?Leu?Leu?Leu?Lys?Leu?Lys?Gln?Ile?Cys?Asn

835?????????????????840?????????????????845

His?Pro?Ala?His?Phe?Leu?Lys?Gln?Lys?Ser?Leu?Lys?Thr?Ala?Glu?Gln

850?????????????????855?????????????????860

Ser?Gly?Lys?Leu?Leu?Arg?Leu?Glu?Glu?Met?Leu?Glu?Glu?Leu?Ile?Glu

865?????????????????870?????????????????875?????????????????880

Glu?Gly?Asp?His?Ala?Leu?Ile?Phe?Thr?Gln?Phe?Ser?Glu?Trp?Gly?Lys

885?????????????????890?????????????????895

Leu?Leu?Gln?Pro?Tyr?Leu?Gln?Lys?Lys?Phe?Gln?Gln?Asp?Val?Leu?Phe

900?????????????????905?????????????????910

Leu?Tyr?Gly?Ala?Thr?Arg?Arg?Val?Gln?Arg?Gln?Glu?Met?Ile?Asp?Arg

915?????????????????920?????????????????925

Phe?Gln?Gln?Asp?Pro?Asn?Gly?Pro?Arg?Ile?Phe?Ile?Leu?Ser?Leu?Lys

930?????????????????935?????????????????940

Ala?Gly?Gly?Thr?Gly?Leu?Asn?Leu?Thr?Arg?Ala?Asn?His?Val?Phe?His

945?????????????????950?????????????????955?????????????????960

Ile?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp?Arg

965?????????????????970?????????????????975

Ala?Phe?Arg?Leu?Gly?Gln?Lys?Arg?Asn?Val?Gln?Val?His?Lys?Phe?Val

980?????????????????985?????????????????990

Cys?Thr?Gly?Thr?Leu?Glu?Glu?Lys??Ile?Asn?Glu?Met?Leu??Glu?Ser?Lys

995?????????????????1000?????????????????1005

Gln?Lys??Leu?Ala?Glu?Gln?Thr??Val?Asp?Ala?Gly?Glu??Gln?Trp?Leu

1010?????????????????1015?????????????????1020

Thr?Glu??Leu?Asp?Thr?Asp?Gln??Leu?Arg?Asn?Leu?Leu??Leu?Leu?Asp

1025?????????????????1030?????????????????1035

Arg?Asp??Thr?Ile?Ile?Asp?Glu??Gln

1040?????????????????1045

<210>39

<211>3027

<212>DNA

＜213〉purple Bacillus adhaerens

<400>39

atggctatct?tgcacggtat?ctgggttcac?caaccccccc?gggccgggct?tttcctttgg????60

ggagaaacct?ggaggcaggt?cgcaaagcgg?cgcaagcgct?ccgaagcacc?cgctccgcat????120

ccctatgtcc?agcaaccggc?cgagttgtcc?ccccgcctgg?ctgcccagtt?tccccagata????180

ccgctcagct?tgctggtacc?cgagacgctt?gcactccagt?tgcccgccac?ggtcgaaaac????240

gtggtctact?ccgcaagcat?tgctcccgag?ggcaagcttt?tggagttgga?accgtggctg????300

gtggaaggtt?tctggctcga?cggtcaccag?gcttttgaac?tgttgctcgg?ggtacccctg????360

ggcggcgggg?acgcatcgat?tggcgacgac?ctgcgcttct?ggtcgcagtg?cgcccgctgg????420

gtgcttgact?tgctggtgcg?cgccaagtac?ctgcccgacc?tggagagcgg?cgacggccag????480

gaaatcccca?cagcccgctg?ggtgcccctg?ctcgacagcg?ccgtcgatca?agcccgcctc????540

aaagaatttg?ccgcccgttt?gccgggcgcc?tgccgcgccg?ctacccccga?actatctccg????600

caccagattc?tcaagagttt?cctgagcgcc?atgctcgacg?cgcgggtgcg?cacgctgctc????660

gcttgcgagc?ctcccgatcc?gcgcacgctg?cctgccggag?cggtgcgccc?ctggcttctg????720

gccctggccc?atgcccagcc?ccagctcaaa?tctccggacc?cggagacgcc?ggctctggcg????780

gaagccctgg?ccacctggcg?cgcccccctg?agctatcagg?ttcgctcgcg?cacctgcttc????840

cgtctgcagc?cgcccgagga?gagccagggc?gagtggaagc?tgcactttct?attgcaaaca????900

ggcgacgatc?ccgattcgct?gatggctgcc?cagcaagtct?ggagcagcgc?gggtgagctg????960

caggaggtgt?ttctcgcggg?cttgggcctc?gcctcgcgta?tctttgtgcc?cgtcgagcgg???1020

ggattgctcg?tcccccagcc?cacctgctgc?accatgagca?ccgtcgaggc?gtttcagttt???1080

ctcaaagccg?ccacctggcg?gttgcgcgac?agcggcttcg?gggtgttgtt?gcccgagagc???1140

ctcgcggacg?cgggcagcct?gcgcaaccgc?ctgggcctca?aactcgaagc?gaacgcgccg???1200

gggcgcaacg?gttcgggcct?cggcatgcag?agcttgctcg?cttttaaatg?ggagctgtcg???1260

ctcgcgggca?agaccctgag?ccgcgccgag?ttcgaccgcc?tcgccgctag?ttctgaaccc???1320

ctggtcaaag?tcaacgacaa?ctgggtcgaa?ttgcgccccc?aggacgtgcg?cgccgcccac???1380

agctttttgc?agtcgcgcaa?agatcaggtc?ggactctcgt?tggaggatgt?gctgcgcctc????1440

aacttcggcg?acacccccaa?aatcgacggt?ctccccatcg?tcaacttcga?cagctccggc????1500

cccattcagc?aactgctgga?gaccctcacc?gatcagcgca?aactcacccc?catcgacgaa????1560

ccgccggggt?tcaagggcac?cctgcggccc?tatcaaaaaa?ttggcgtcgg?ctggctcgcc????1620

tttttgcaga?agtggggcct?gggtgcttgc?ctagccgacg?acatgggact?cgggaagacc????1680

gtagagttga?tagcatttct?tctttttctc?aaatccaaaa?atgagctgga?cggccctata????1740

ttgttaattt?gtccgacttc?agtgatggga?aactgggaaa?gagaaataaa?gaaattttct????1800

cctagtttat?ctgtacatgt?ccatcatggg?gcgcggcggc?cgaaggggcg?caattttgtc????1860

gagacggccc?agaaaaagca?aatcatcgtc?agcagctacg?ccctggtaca?gcgcgacagc????1920

aaagatctca?agcgcgtcga?atggttgggc?ctggtgctcg?acgaagccca?gaacatcaaa????1980

aaccccgacg?ccaagcagac?ccagtcgatt?cgggaactga?cagcgcgctt?tcgcatcgcc????2040

ctcaccggca?caccggtcga?gaatcgcctc?gcggaactgt?ggtcgatcct?cgattttctc????2100

aatcccggct?atctgggggc?gcgcaacttc?tttcagcgcc?gcttcgcagt?tccgatcgaa????2160

aagtacgggg?atcgctcctc?ggcgaacgcc?ctcaaagctc?tggtgcagcc?gtttatcctg????2220

cggcggctca?aatccgaccc?gcagattatt?caagatctgc?ccgagaagca?ggagacgaat????2280

gtcttctgtc?cgctcacacc?cgagcaggcg?gccctctacg?agcgggtggt?gaacgaatcg????2340

ctcgccaaga?tcgagcagag?caccggcatc?cagcggcgcg?ggacggtgct?ggccaccttg????2400

gtcaaactca?agcagatctg?caaccacccg?agccactacc?tgggtgacga?cggaccgctc????2460

gccaaccgct?cgggcaaact?cagccgcctg?ggcgagatgc?tcgaagaagt?gctcgccgac????2520

gaggagcggg?cgctgatttt?tacccagttc?gccgagtggg?gccacctgct?gcaggcgcac????2580

ctgagccgcc?agttgggttc?agaagtgttt?ttcctctacg?gcggcaccag?caaaaaccag????2640

cgcgaggcga?tgatcgagcg?cttccagagc?gatccgcagg?ggccgcggat?ttttattctt????2700

tcgctgaagg?cagggggtgt?cggcctcaac?ctcacccgcg?ccaaccacgt?cttccacttc??2760

gaccgctggt?ggaacccggc?ggtcgagaat?caggccaccg?accgcgtctt?ccgcatcggc??2820

caaaccaaga?acgtacaagt?ctacaagtac?gtgtgcaccg?gcacgctcga?agagcgcatc??2880

aacgccctga?tcgaaagcaa?aaaggccctg?gctgagcagg?tggtgagcgc?cggtgagaac??2940

tggctgtcgg?atctaaatac?cgatcaactg?cggcaactgt?tggtactcga?tcgctcggag??3000

attatcgaca?cggaggacac?cgcgtga??????????????????????????????????????3027

<210>40

<211>1008

<212>PRT

＜213〉purple Bacillus adhaerens

<400>40

Met?Ala?Ile?Leu?His?Gly?Ile?Trp?Val?His?Gln?Pro?Pro?Arg?Ala?Gly

1???????????????5???????????????????10??????????????????15

Leu?Phe?Leu?Trp?Gly?Glu?Thr?Trp?Arg?Gln?Val?Ala?Lys?Arg?Arg?Lys

20??????????????????25??????????????????30

Arg?Ser?Glu?Ala?Pro?Ala?Pro?His?Pro?Tyr?Val?Gln?Gln?Pro?Ala?Glu

35??????????????????40??????????????????45

Leu?Ser?Pro?Arg?Leu?Ala?Ala?Gln?Phe?Pro?Gln?Ile?Pro?Leu?Ser?Leu

50??????????????????55??????????????????60

Leu?Val?Pro?Glu?Thr?Leu?Ala?Leu?Gln?Leu?Pro?Ala?Thr?Val?Glu?Asn

65??????????????????70??????????????????75??????????????????80

Val?Val?Tyr?Ser?Ala?Ser?Ile?Ala?Pro?Glu?Gly?Lys?Leu?Leu?Glu?Leu

85??????????????????90??????????????????95

Glu?Pro?Trp?Leu?Val?Glu?Gly?Phe?Trp?Leu?Asp?Gly?His?Gln?Ala?Phe

100?????????????????105?????????????????110

Glu?Leu?Leu?Leu?Gly?Val?Pro?Leu?Gly?Gly?Gly?Asp?Ala?Ser?Ile?Gly

115?????????????????120?????????????????125

Asp?Asp?Leu?Arg?Phe?Trp?Ser?Gln?Cys?Ala?Arg?Trp?Val?Leu?Asp?Leu

130?????????????????135?????????????????140

Leu?Val?Arg?Ala?Lys?Tyr?Leu?Pro?Asp?Leu?Glu?Ser?Gly?Asp?Gly?Gln

145?????????????????150?????????????????155?????????????????160

Glu?Ile?Pro?Thr?Ala?Arg?Trp?Val?Pro?Leu?Leu?Asp?Ser?Ala?Val?Asp

165?????????????????170?????????????????175

Gln?Ala?Arg?Leu?Lys?Glu?Phe?Ala?Ala?Arg?Leu?Pro?Gly?Ala?Cys?Arg

180?????????????????185?????????????????190

Ala?Ala?Thr?Pro?Glu?Leu?Ser?Pro?His?Gln?Ile?Leu?Lys?Ser?Phe?Leu

195?????????????????200?????????????????205

Ser?Ala?Met?Leu?Asp?Ala?Arg?Val?Arg?Thr?Leu?Leu?Ala?Cys?Glu?Pro

210?????????????????215?????????????????220

Pro?Asp?Pro?Arg?Thr?Leu?Pro?Ala?Gly?Ala?Val?Arg?Pro?Trp?Leu?Leu

225?????????????????230?????????????????235?????????????????240

Ala?Leu?Ala?His?Ala?Gln?Pro?Gln?Leu?Lys?Ser?Pro?Asp?Pro?Glu?Thr

245?????????????????250?????????????????255

Pro?Ala?Leu?Ala?Glu?Ala?Leu?Ala?Thr?Trp?Arg?Ala?Pro?Leu?Ser?Tyr

260?????????????????265?????????????????270

Gln?Val?Arg?Ser?Arg?Thr?Cys?Phe?Arg?Leu?Gln?Pro?Pro?Glu?Glu?Ser

275?????????????????280?????????????????285

Gln?Gly?Glu?Trp?Lys?Leu?His?Phe?Leu?Leu?Gln?Thr?Gly?Asp?Asp?Pro

290?????????????????295?????????????????300

Asp?Ser?Leu?Met?Ala?Ala?Gln?Gln?Val?Trp?Ser?Ser?Ala?Gly?Glu?Leu

305?????????????????310?????????????????315?????????????????320

Gln?Glu?Val?Phe?Leu?Ala?Gly?Leu?Gly?Leu?Ala?Ser?Arg?Ile?Phe?Val

325?????????????????330?????????????????335

Pro?Val?Glu?Arg?Gly?Leu?Leu?Val?Pro?Gln?Pro?Thr?Cys?Cys?Thr?Met

340?????????????????345?????????????????350

Ser?Thr?Val?Glu?Ala?Phe?Gln?Phe?Leu?Lys?Ala?Ala?Thr?Trp?Arg?Leu

355?????????????????360?????????????????365

Arg?Asp?Ser?Gly?Phe?Gly?Val?Leu?Leu?Pro?Glu?Ser?Leu?Ala?Asp?Ala

370?????????????????375?????????????????380

Gly?Ser?Leu?Arg?Asn?Arg?Leu?Gly?Leu?Lys?Leu?Glu?Ala?Asn?Ala?Pro

385?????????????????390?????????????????395?????????????????400

Gly?Arg?Asn?Gly?Ser?Gly?Leu?Gly?Met?Gln?Ser?Leu?Leu?Ala?Phe?Lys

405?????????????????410?????????????????415

Trp?Glu?Leu?Ser?Leu?Ala?Gly?Lys?Thr?Leu?Ser?Arg?Ala?Glu?Phe?Asp

420?????????????????425?????????????????430

Arg?Leu?Ala?Ala?Ser?Ser?Glu?Pro?Leu?Val?Lys?Val?Asn?Asp?Asn?Trp

435?????????????????440?????????????????445

Val?Glu?Leu?Arg?Pro?Gln?Asp?Val?Arg?Ala?Ala?His?Ser?Phe?Leu?Gln

450?????????????????455?????????????????460

Ser?Arg?Lys?Asp?Gln?Val?Gly?Leu?Ser?Leu?Glu?Asp?Val?Leu?Arg?Leu

465?????????????????470?????????????????475?????????????????480

Asn?Phe?Gly?Asp?Thr?Pro?Lys?Ile?Asp?Gly?Leu?Pro?Ile?Val?Asn?Phe

485?????????????????490?????????????????495

Asp?Ser?Ser?Gly?Pro?Ile?Gln?Gln?Leu?Leu?Glu?Thr?Leu?Thr?Asp?Gln

500?????????????????505?????????????????510

Arg?Lys?Leu?Thr?Pro?Ile?Asp?Glu?Pro?Pro?Gly?Phe?Lys?Gly?Thr?Leu

515?????????????????520?????????????????525

Arg?Pro?Tyr?Gln?Lys?Ile?Gly?Val?Gly?Trp?Leu?Ala?Phe?Leu?Gln?Lys

530?????????????????535?????????????????540

Trp?Gly?Leu?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr

545?????????????????550?????????????????555?????????????????560

Val?Glu?Leu?Ile?Ala?Phe?Leu?Leu?Phe?Leu?Lys?Ser?Lys?Asn?Glu?Leu

565?????????????????570?????????????????575

Asp?Gly?Pro?Ile?Leu?Leu?Ile?Cys?Pro?Thr?Ser?Val?Met?Gly?Asn?Trp

580?????????????????585?????????????????590

Glu?Arg?Glu?Ile?Lys?Lys?Phe?Ser?Pro?Ser?Leu?Ser?Val?His?Val?His

595?????????????????600?????????????????605

His?Gly?Ala?Arg?Arg?Pro?Lys?Gly?Arg?Asn?Phe?Val?Glu?Thr?Ala?Gln

610?????????????????615?????????????????620

Lys?Lys?Gln?Ile?Ile?Val?Ser?Ser?Tyr?Ala?Leu?Val?Gln?Arg?Asp?Ser

625?????????????????630?????????????????635?????????????????640

Lys?Asp?Leu?Lys?Arg?Val?Glu?Trp?Leu?Gly?Leu?Val?Leu?Asp?Glu?Ala

645?????????????????650?????????????????655

Gln?Asn?Ile?Lys?Asn?Pro?Asp?Ala?Lys?Gln?Thr?Gln?Ser?Ile?Arg?Glu

660?????????????????665?????????????????670

Leu?Thr?Ala?Arg?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn

675?????????????????680?????????????????685

Arg?Leu?Ala?Glu?Leu?Trp?Ser?Ile?Leu?Asp?Phe?Leu?Asn?Pro?Gly?Tyr

690?????????????????695?????????????????700

Leu?Gly?Ala?Arg?Asn?Phe?Phe?Gln?Arg?Arg?Phe?Ala?Val?Pro?Ile?Glu

705?????????????????710?????????????????715?????????????????720

Lys?Tyr?Gly?Asp?Arg?Ser?Ser?Ala?Asn?Ala?Leu?Lys?Ala?Leu?Val?Gln

725?????????????????730?????????????????735

Pro?Phe?Ile?Leu?Arg?Arg?Leu?Lys?Ser?Asp?Pro?Gln?Ile?Ile?Gln?Asp

740?????????????????745?????????????????750

Leu?Pro?Glu?Lys?Gln?Glu?Thr?Asn?Val?Phe?Cys?Pro?Leu?Thr?Pro?Glu

755?????????????????760?????????????????765

Gln?Ala?Ala?Leu?Tyr?Glu?Arg?Val?Val?Asn?Glu?Ser?Leu?Ala?Lys?Ile

770?????????????????775?????????????????780

Glu?Gln?Ser?Thr?Gly?Ile?Gln?Arg?Arg?Gly?Thr?Val?Leu?Ala?Thr?Leu

785?????????????????790?????????????????795?????????????????800

Val?Lys?Leu?Lys?Gln?Ile?Cys?Asn?His?Pro?Ser?His?Tyr?Leu?Gly?Asp

805?????????????????810?????????????????815

Asp?Gly?Pro?Leu?Ala?Asn?Arg?Ser?Gly?Lys?Leu?Ser?Arg?Leu?Gly?Glu

820?????????????????825?????????????????830

Met?Leu?Glu?Glu?Val?Leu?Ala?Asp?Glu?Glu?Arg?Ala?Leu?Ile?Phe?Thr

835?????????????????840?????????????????845

Gln?Phe?Ala?Glu?Trp?Gly?His?Leu?Leu?Gln?Ala?His?Leu?Ser?Arg?Gln

850?????????????????855?????????????????860

Leu?Gly?Ser?Glu?Val?Phe?Phe?Leu?Tyr?Gly?Gly?Thr?Ser?Lys?Asn?Gln

865?????????????????870?????????????????875?????????????????880

Arg?Glu?Ala?Met?Ile?Glu?Arg?Phe?Gln?Ser?Asp?Pro?Gln?Gly?Pro?Arg

885?????????????????890?????????????????895

Ile?Phe?Ile?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr

900?????????????????905?????????????????910

Arg?Ala?Asn?His?Val?Phe?His?Phe?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val

915?????????????????920?????????????????925

Glu?Asn?Gln?Ala?Thr?Asp?Arg?Val?Phe?Arg?Ile?Gly?Gln?Thr?Lys?Asn

930?????????????????935?????????????????940

Val?Gln?Val?Tyr?Lys?Tyr?Val?Cys?Thr?Gly?Thr?Leu?Glu?Glu?Arg?Ile

945?????????????????950?????????????????955?????????????????960

Asn?Ala?Leu?Ile?Glu?Ser?Lys?Lys?Ala?Leu?Ala?Glu?Gln?Val?Val?Ser

965?????????????????970?????????????????975

Ala?Gly?Glu?Asn?Trp?Leu?Ser?Asp?Leu?Asn?Thr?Asp?Gln?Leu?Arg?Gln

980?????????????????985?????????????????990

Leu?Leu?Val?Leu?Asp?Arg?Ser?Glu??Ile?Ile?Asp?Thr?Glu??Asp?Thr?Ala

995?????????????????1000?????????????????1005

<210>41

<211>3186

<212>DNA

＜213〉sheath silk algae

<400>41

atggcaattt?tacacggaag?ttggctccag?caccccaaaa?attatttgtt?tatttgggga?????60

gaaacctggc?gtcgcattac?acccaatgaa?tttaatccgg?ctgatggtgt?tttgggttat????120

ccttttgctt?taagccctgt?tgaattggaa?aagtggtgca?gtgaaaagca?gttatctata????180

gagagtaaag?ttgtcgttac?agaaactctc?gcccttccca?ctaaactctc?cccaaaaata????240

ggactatatc?cccttcaatc?tacgcctcaa?actgattctg?aaactgattc?tgagtcgatc????300

tgtctttatc?cctggaaaat?tgaaggtatt?tgtctcaaca?gtacagaagc?ctttgacttt????360

ttacaatccc?ttcctctggg?aaacctgacc?acagaaaact?catttattgg?ctcagattta????420

cagttttggt?ctcatctttc?ccgttggagt?ttagacttac?tcgcccggag?taaattttta????480

cccagtctca?cttttaaccc?ctcaaaagat?cactttatcg?ctgaatggaa?acctttactc????540

gatagtgcga?cagatcaagc?cagattaatt?cgtttttcta?aacaaatacc?ctctgcttgt????600

cggatctatc?aactctggtc?aaaagaggct?caaaatcaat?ttgaaaattt?agccctagat????660

ttacctcaaa?atccccaaaa?cttaattgat?gattttttaa?cggcaattat?tgatagtcaa????720

gtcaagaaag?ttgcagaaga?aagtgaaaaa?aaagcgatta?caaatctaac?cgctattcaa????780

ccgattgttc?agagttggtt?acacgcttta?gccagtgaat?ctaatctagc?aaaatccaaa????840

aaatctgaat?caaaaaccct?agaaaaaatt?ctttccaatt?ggacggctcc?tcttcaacaa????900

actctcgctg?aacataattt?gtttagaacg?ggatttcgac?tctctcctcc?ggaaaataat?????960

caaaaaaatt?ggacgctaga?ttattgttta?caagcaattg?atgaacccga?atttttagtg????1020

gatgctcaaa?ctatttggac?tcatccagtc?gaagcctttg?ttcacaatgg?acgtatgatt????1080

aaacgtcctc?aagaaaccct?cctcaaaggt?ttaggtttag?cctcaaaact?atatcctctc????1140

ctagaaccca?gtttacaaga?agcccgtcct?caaacttgct?tattaacgcc?cctacaagcc????1200

tatgaattta?ttaaaagtat?taattggcgg?tttacagata?gcggtttagg?agtgatttta????1260

cccccgagtt?tagtcagtca?aaatggatgg?gcgaaccgtt?taggtttaag?tgttcaagcg????1320

gcgacatcaa?aatccaaaca?aaatgttagc?ttgggattag?atagtctgct?gaattttaaa????1380

tgggaattgt?caattggggg?tcaaacctta?tcaaaaacag?aatttaaccg?tttagtcgct????1440

caagaaagtc?cgttagttga?aattaatggc?gaatgggtgg?aattacgtcc?tactgatatt????1500

aaagccgcta?aagccttctt?ttcgagtcgc?aaagatcaac?tttcacttac?ccttgaagat????1560

gctttacgtt?tatcgacggg?tgactcgcaa?atggtggaaa?agttaccgat?tgttaacttt????1620

gaagcgggtg?gaaaattaga?agaacttctc?aatactttaa?cgaataaccg?ttcgctcgat????1680

gagatcaaaa?ctcctagtaa?ttttcaagga?gaactacgcc?cctatcaagc?ccgaggggtg????1740

agttggttag?cctttttaga?agaatggggt?ttaggggctt?gtttagctga?tgatatgggg????1800

ctaggaaaaa?ccatagaatt?aattgctttt?ctcttgtatt?tgcaggaaaa?agaaacctta????1860

gacgctcctg?ttttactggt?ttgtccgaca?tcagttttag?gaaactggga?acgagaagtt????1920

aaacgattta?gtccgagttt?aaaagttact?gttcatcacg?gggataaacg?ccagaaaggg????1980

aaaaactttg?ctcaatttgc?ccagaaatat?aatttaatta?ttaccagtta?tccgttaact????2040

tttcgagatg?agaaagaact?caaaacggta?aattggaaag?gattagtttt?agacgaagct????2100

caaaatatta?aaaatcccga?ggctaaacaa?tcaaaaacgg?tgagaaatct?acaggcgagt????2160

tttaaaattg?ctctgactgg?aacacctgtc?gaaaaccgtc?tgtctgaatt?atggtcaatt????2220

atggattttc?tcaacccagg?ttatttagga?cagcgacaat?tttttcagcg?aagatttgct??2280

attccgattg?aaaaatacgg?cgatacagac?tccttaaaaa?cattgcgatc?tttggttcaa??2340

ccgtttattt?tacggcgctt?aaaaacagat?agagagatta?tccaagactt?acccgaaaaa??2400

caggaaaata?cgatcttttg?ttctctgtct?acagaacaag?caacgcttta?tcaaaagatt??2460

gttgatcagt?ctttagctga?catagactca?gccgcaggaa?ttcaacgtcg?agggatgatt??2520

ttagcgttgt?tagtgaaatt?aaaacaggtt?tgtaatcatc?ccattttatt?gaatggaaaa??2580

gcgacaaaaa?ctggaaagaa?aaaggtcgag?actcagggtt?taagcctgca?aagttcaggg??2640

aagttacaac?gcttcaaaga?aatgctggaa?gaattgttgt?cagaaggaga?tcgcgccatt??2700

gtatttaccc?agtttgcaga?atggggaaaa?gttttacaac?cttatttaga?acagcaatta??2760

aaccgagagg?tattattttt?gtatggcgca?actcgtaaaa?ataaacgaga?agaaatgatt??2820

gatcgttttc?aacaagatcc?tcaagggcca?ccgattttta?ttctatcttt?aaaagcggga??2880

ggtgtgggtt?taaatttgac?tcgtgctaat?catgtttttc?actttgatcg?ttggtggaac??2940

cctgcggttg?aaaatcaagc?aacagatcgg?gtgtttagaa?ttggtcaaac?gcgcaatgtt??3000

caggttcata?agtttgtctg?taccggaacg?ttggaagaaa?aaatccatga?tttaattgaa??3060

agtaaaaaag?tgttggctga?acaagttgtg?ggttcaggag?aaaattggtt?aactgaattg??3120

gatacggatc?aactcagaaa?cttactcatt?attgaccgaa?atgcggtgat?tgatgaagaa??3180

gaataa?????????????????????????????????????????????????????????????3186

<210>42

<211>1061

<212>PRT

＜213〉sheath silk algae

<400>42

Met?Ala?Ile?Leu?His?Gly?Ser?Trp?Leu?Gln?His?Pro?Lys?Asn?Tyr?Leu

1???????????????5???????????????????10??????????????????15

Phe?Ile?Trp?Gly?Glu?Thr?Trp?Arg?Arg?Ile?Thr?Pro?Asn?Glu?Phe?Asn

20??????????????????25??????????????????30

Pro?Ala?Asp?Gly?Val?Leu?Gly?Tyr?Pro?Phe?Ala?Leu?Ser?Pro?Val?Glu

35??????????????????40??????????????????45

Leu?Glu?Lys?Trp?Cys?Ser?Glu?Lys?Gln?Leu?Ser?Ile?Glu?Ser?Lys?Val

50??????????????????55??????????????????60

Val?Val?Thr?Glu?Thr?Leu?Ala?Leu?Pro?Thr?Lys?Leu?Ser?Pro?Lys?Ile

65??????????????????70??????????????????75??????????????????80

Gly?Leu?Tyr?Pro?Leu?Gln?Ser?Thr?Pro?Gln?Thr?Asp?Ser?Glu?Thr?Asp

85??????????????????90??????????????????95

Ser?Glu?Ser?Ile?Cys?Leu?Tyr?Pro?Trp?Lys?Ile?Glu?Gly?Ile?Cys?Leu

100?????????????????105?????????????????110

Asn?Ser?Thr?Glu?Ala?Phe?Asp?Phe?Leu?Gln?Ser?Leu?Pro?Leu?Gly?Asn

115?????????????????120?????????????????125

Leu?Thr?Thr?Glu?Asn?Ser?Phe?Ile?Gly?Ser?Asp?Leu?Gln?Phe?Trp?Ser

130?????????????????135?????????????????140

His?Leu?Ser?Arg?Trp?Ser?Leu?Asp?Leu?Leu?Ala?Arg?Ser?Lys?Phe?Leu

145?????????????????150?????????????????155?????????????????160

Pro?Ser?Leu?Thr?Phe?Asn?Pro?Ser?Lys?Asp?His?Phe?Ile?Ala?Glu?Trp

165?????????????????170?????????????????175

Lys?Pro?Leu?Leu?Asp?Ser?Ala?Thr?Asp?Gln?Ala?Arg?Leu?Ile?Arg?Phe

180?????????????????185?????????????????190

Ser?Lys?Gln?Ile?Pro?Ser?Ala?Cys?Arg?Ile?Tyr?Gln?Leu?Trp?Ser?Lys

195?????????????????200?????????????????205

Glu?Ala?Gln?Asn?Gln?Phe?Glu?Asn?Leu?Ala?Leu?Asp?Leu?Pro?Gln?Asn

210?????????????????215?????????????????220

Pro?Gln?Asn?Leu?Ile?Asp?Asp?Phe?Leu?Thr?Ala?Ile?Ile?Asp?Ser?Gln

225?????????????????230?????????????????235?????????????????240

Val?Lys?Lys?Val?Ala?Glu?Glu?Ser?Glu?Lys?Lys?Ala?Ile?Thr?Asn?Leu

245?????????????????250?????????????????255

Thr?Ala?Ile?Gln?Pro?Ile?Val?Gln?Ser?Trp?Leu?His?Ala?Leu?Ala?Ser

260?????????????????265?????????????????270

Glu?Ser?Asn?Leu?Ala?Lys?Ser?Lys?Lys?Ser?Glu?Ser?Lys?Thr?Leu?Glu

275?????????????????280?????????????????285

Lys?Ile?Leu?Ser?Asn?Trp?Thr?Ala?Pro?Leu?Gln?Gln?Thr?Leu?Ala?Glu

290?????????????????295?????????????????300

His?Asn?Leu?Phe?Arg?Thr?Gly?Phe?Arg?Leu?Ser?Pro?Pro?Glu?Asn?Asn

305?????????????????310?????????????????315?????????????????320

Gln?Lys?Asn?Trp?Thr?Leu?Asp?Tyr?Cys?Leu?Gln?Ala?Ile?Asp?Glu?Pro

325?????????????????330?????????????????335

Glu?Phe?Leu?Val?Asp?Ala?Gln?Thr?Ile?Trp?Thr?His?Pro?Val?Glu?Ala

340?????????????????345?????????????????350

Phe?Val?His?Asn?Gly?Arg?Met?Ile?Lys?Arg?Pro?Gln?Glu?Thr?Leu?Leu

355?????????????????360?????????????????365

Lys?Gly?Leu?Gly?Leu?Ala?Ser?Lys?Leu?Tyr?Pro?Leu?Leu?Glu?Pro?Ser

370?????????????????375?????????????????380

Leu?Gln?Glu?Ala?Arg?Pro?Gln?Thr?Cys?Leu?Leu?Thr?Pro?Leu?Gln?Ala

385?????????????????390?????????????????395?????????????????400

Tyr?Glu?Phe?Ile?Lys?Ser?Ile?Asn?Trp?Arg?Phe?Thr?Asp?Ser?Gly?Leu

405?????????????????410?????????????????415

Gly?Val?Ile?Leu?Pro?Pro?Ser?Leu?Val?Ser?Gln?Asn?Gly?Trp?Ala?Asn

420?????????????????425?????????????????430

Arg?Leu?Gly?Leu?Ser?Val?Gln?Ala?Ala?Thr?Ser?Lys?Ser?Lys?Gln?Asn

435?????????????????440?????????????????445

Val?Ser?Leu?Gly?Leu?Asp?Ser?Leu?Leu?Asn?Phe?Lys?Trp?Glu?Leu?Ser

450?????????????????455?????????????????460

Ile?Gly?Gly?Gln?Thr?Leu?Ser?Lys?Thr?Glu?Phe?Asn?Arg?Leu?Val?Ala

465?????????????????470?????????????????475?????????????????480

Gln?Glu?Ser?Pro?Leu?Val?Glu?Ile?Asn?Gly?Glu?Trp?Val?Glu?Leu?Arg

485?????????????????490?????????????????495

Pro?Thr?Asp?Ile?Lys?Ala?Ala?Lys?Ala?Phe?Phe?Ser?Ser?Arg?Lys?Asp

500?????????????????505?????????????????510

Gln?Leu?Ser?Leu?Thr?Leu?Glu?Asp?Ala?Leu?Arg?Leu?Ser?Thr?Gly?Asp

515?????????????????520?????????????????525

Ser?Gln?Met?Val?Glu?Lys?Leu?Pro?Ile?Val?Asn?Phe?Glu?Ala?Gly?Gly

530?????????????????535?????????????????540

Lys?Leu?Glu?Glu?Leu?Leu?Asn?Thr?Leu?Thr?Asn?Asn?Arg?Ser?Leu?Asp

545?????????????????550?????????????????555?????????????????560

Glu?Ile?Lys?Thr?Pro?Ser?Asn?Phe?Gln?Gly?Glu?Leu?Arg?Pro?Tyr?Gln

565?????????????????570?????????????????575

Ala?Arg?Gly?Val?Ser?Trp?Leu?Ala?Phe?Leu?Glu?Glu?Trp?Gly?Leu?Gly

580?????????????????585?????????????????590

Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Glu?Leu?Ile

595?????????????????600?????????????????605

Ala?Phe?Leu?Leu?Tyr?Leu?Gln?Glu?Lys?Glu?Thr?Leu?Asp?Ala?Pro?Val

610?????????????????615?????????????????620

Leu?Leu?Val?Cys?Pro?Thr?Ser?Val?Leu?Gly?Asn?Trp?Glu?Arg?Glu?Val

625?????????????????630?????????????????635?????????????????640

Lys?Arg?Phe?Ser?Pro?Ser?Leu?Lys?Val?Thr?Val?His?His?Gly?Asp?Lys

645?????????????????650?????????????????655

Arg?Gln?Lys?Gly?Lys?Asn?Phe?Ala?Gln?Phe?Ala?Gln?Lys?Tyr?Asn?Leu

660?????????????????665?????????????????670

Ile?Ile?Thr?Ser?Tyr?Pro?Leu?Thr?Phe?Arg?Asp?Glu?Lys?Glu?Leu?Lys

675?????????????????680?????????????????685

Thr?Val?Asn?Trp?Lys?Gly?Leu?Val?Leu?Asp?Glu?Ala?Gln?Asn?Ile?Lys

690?????????????????695?????????????????700

Asn?Pro?Glu?Ala?Lys?Gln?Ser?Lys?Thr?Val?Arg?Asn?Leu?Gln?Ala?Ser

705?????????????????710?????????????????715?????????????????720

Phe?Lys?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu?Ser?Glu

725?????????????????730?????????????????735

Leu?Trp?Ser?Ile?Met?Asp?Phe?Leu?Asn?Pro?Gly?Tyr?Leu?Gly?Gln?Arg

740?????????????????745?????????????????750

Gln?Phe?Phe?Gln?Arg?Arg?Phe?Ala?Ile?Pro?Ile?Glu?Lys?Tyr?Gly?Asp

755?????????????????760?????????????????765

Thr?Asp?Ser?Leu?Lys?Thr?Leu?Arg?Ser?Leu?Val?Gln?Pro?Phe?Ile?Leu

770?????????????????775?????????????????780

Arg?Arg?Leu?Lys?Thr?Asp?Arg?Glu?Ile?Ile?Gln?Asp?Leu?Pro?Glu?Lys

785?????????????????790?????????????????795?????????????????800

Gln?Glu?Asn?Thr?Ile?Phe?Cys?Ser?Leu?Ser?Thr?Glu?Gln?Ala?Thr?Leu

805?????????????????810?????????????????815

Tyr?Gln?Lys?Ile?Val?Asp?Gln?Ser?Leu?Ala?Asp?Ile?Asp?Ser?Ala?Ala

820?????????????????825?????????????????830

Gly?Ile?Gln?Arg?Arg?Gly?Met?Ile?Leu?Ala?Leu?Leu?Val?Lys?Leu?Lys

835?????????????????840?????????????????845

Gln?Val?Cys?Asn?His?Pro?Ile?Leu?Leu?Asn?Gly?Lys?Ala?Thr?Lys?Thr

850?????????????????855?????????????????860

Gly?Lys?Lys?Lys?Val?Glu?Thr?Gln?Gly?Leu?Ser?Leu?Gln?Ser?Ser?Gly

865?????????????????870?????????????????875?????????????????880

Lys?Leu?Gln?Arg?Phe?Lys?Glu?Met?Leu?Glu?Glu?Leu?Leu?Ser?Glu?Gly

885?????????????????890?????????????????895

Asp?Arg?Ala?Ile?Val?Phe?Thr?Gln?Phe?Ala?Glu?Trp?Gly?Lys?Val?Leu

900?????????????????905?????????????????910

Gln?Pro?Tyr?Leu?Glu?Gln?Gln?Leu?Asn?Arg?Glu?Val?Leu?Phe?Leu?Tyr

915?????????????????920?????????????????925

Gly?Ala?Thr?Arg?Lys?Asn?Lys?Arg?Glu?Glu?Met?Ile?Asp?Arg?Phe?Gln

930?????????????????935?????????????????940

Gln?Asp?Pro?Gln?Gly?Pro?Pro?Ile?Phe?Ile?Leu?Ser?Leu?Lys?Ala?Gly

945?????????????????950?????????????????955?????????????????960

Gly?Val?Gly?Leu?Asn?Leu?Thr?Arg?Ala?Asn?His?Val?Phe?His?Phe?Asp

965?????????????????970?????????????????975

Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp?Arg?Val?Phe

980?????????????????985?????????????????990

Arg?Ile?Gly?Gln?Thr?Arg?Asn?Val??Gln?Val?His?Lys?Phe??Val?Cys?Thr

995?????????????????1000?????????????????1005

Gly?Thr??Leu?Glu?Glu?Lys?Ile??His?Asp?Leu?Ile?Glu??Ser?Lys?Lys

1010?????????????????1015?????????????????1020

Val?Leu??Ala?Glu?Gln?Val?Val??Gly?Ser?Gly?Glu?Asn??Trp?Leu?Thr

1025?????????????????1030?????????????????1035

Glu?Leu??Asp?Thr?Asp?Gln?Leu??Arg?Asn?Leu?Leu?Ile??Ile?Asp?Arg

1040?????????????????1045?????????????????1050

Asn?Ala??Val?Ile?Asp?Glu?Glu??Glu

1055?????????????????1060

<210>43

<211>3237

<212>DNA

＜213〉bite the acetate sarcina methanica

<400>43

atgataattt?tgcatgcagg?aagagtcgga?aaacagttct?ttctgtgggg?cgaaagcccg??????60

gctgaaaatg?aaactccgcc?tgtccggcgc?gggagaaagc?ctaagaagcc?ggttgcaaaa?????120

ccttatcctt?acgattcggg?tgttgaaaac?ctgtcttctg?ctcttgagct?gctgctgggc?????180

agtactggcc?ggaaaaaggc?agaggaaatc?aatgtctgga?tcccgacagc?aggctggaat?????240

ccaatcccct?ccagtcctct?cgttgctgaa?attccggctt?cgaaagcaga?actttcccta?????300

gctccctgga?ctgttcacgc?atatcctctg?gaagctgaag?aagctattgt?tctcctctgc?????360

gcctgtatgg?gaaaaaaggt?tcttgctccc?ggcataatct?cgggaaatga?tcttctctgg?????420

tgggcggatg?ccctgaaatt?tgcaggctcg?ctggtagcag?gacagaaata?cctgcctggc?????480

gtcaggggcg?gggaaggaga?gtacaaggct?ttctgggaac?ccgtattttc?cggagaagat?????540

gcgggggagc?tggcaagact?tgcaaagcaa?atgcctccgg?ctgcaaaggc?tcttgctctt?????600

gaaacctctt?ccgtgcagcc?ggaaatactt?gctgctgtag?cggcaaggca?gtttatcgaa?????660

gaggctcttg?actggatagt?ccggtccgag?atcggggaaa?aagagcttgc?aaaagaggcg?????720

cgtaaaagaa?aatcctttga?tagcgtccat?gacgcctggg?tttccgctct?taaaagccct?????780

gacgggttga?tccacggaga?agaaaaagaa?ctcctgcagc?ttgcgttccg?gacccgtgaa?????840

tggcagcgcc?cccttactgt?acttacaact?tctcccttca?ggttctgttt?ccggcttgaa?????900

gagccagctg?cggaagaaga?actcgaagaa?accgaggaat?ccgaagccgg?aaaaatggat?????960

actaaaaaag?gcaggaaagg?gatagctgac?atagaagttc?ccgaagaact?ctggtacgtc????1020

cgctatatgc?ttcagtccta?cgaagaccca?agccttctga?ttcctgtaaa?agaggcctgg????1080

aaaccaaaga?agggcagccc?gttgaaaaga?tatgatgtaa?aaaacattcg?ccaatttctg????1140

ttatcttccc?ttggacaggc?tgctggcatc?agtgcaggaa?ttgcttccag?ccttgaagct????1200

cccaacccgt?ccggatattc?ccttgatacg?aaagaagctt?accgcttcct?gactgaaagt????1260

gcagcggatt?taagccaggc?gggcttcggg?ttacttctcc?ccggctggtg?gacccgtaaa????1320

ggtacaaaga?cccacttaaa?agcccaggct?aatgttaagg?gcaagaagtt?gaaggccgga????1380

tacgggctta?cactcgataa?aatcgtcagc?tttgactggg?aaattgccct?tggagaccgt????1440

gcactcacag?tcagggaact?gcaggctctt?gcaaagctca?aagctccgct?tgtgaaattc????1500

cgcgggcagt?gggtcgaggt?caacgatgcg?gaaatccggg?ctgcccttga?gttctggaag????1560

aaaaaccccc?acggggaagc?aagtctgcgc?gaagttctaa?aactggctgt?gggagtctcc????1620

gaaaaagccg?atggtgtaga?cgttgaaggg?cttaatgcag?ccggctggat?cgaagaatta????1680

atccgccgcc?tgaaggacaa?aaccgggttt?gaagaacttc?cggctcctga?cggtttttca????1740

ggcaccctca?ggccctacca?gttcagaggt?tactcctggc?tggctttcct?gaggcagtgg????1800

ggcataggag?cctgccttgc?agacgacatg?gggcttggta?aaaccatcca?gacccttgcc????1860

cttatccagc?acgacctgga?acaggttaaa?gggcaggttg?aagaaaaggt?tatagaaaat????1920

gctgaagaaa?aagttgaagg?acttaaagct?gcaaaaccgg?ttcttctggt?ctgtccgacc????1980

tctgtcatca?acaactggaa?aaaagaggcg?gctcgcttta?ccccggaact?ttcggtaatg????2040

gtccaccacg?ggaccagccg?gaaaaaggaa?gaggaattca?aaaaggaagc?cacgaatcat????2100

tctattgtcg?tctcaagcta?cgggcttttg?cagcgggatc?ttaagttttt?aaaaggggtt????2160

tcctgggccg?gagtggtact?tgacgaagcc?cagaatatca?aaaacccgga?aaccaaacag????2220

gcaaaggcag?ccagagctct?tgaagccgat?taccgcatag?ctcttacggg?gactccggtt????2280

gaaaacaacg?tgggagacct?ctggtctatc?atggagtttt?taaaccccgg?cttcctaggc????2340

aaccaggcag?gtttcaagcg?gaatttcttt?attcccattc?aggccgaaag?ggatcaggaa????2400

gctgcaagga?ggttaaaaga?aattacgggc?ccctttatcc?tgcgccgtct?gaagaccgat??2460

acttcgatta?tctccgacct?gccggaaaag?atggaaatga?aaacctattg?tacgctgaca??2520

aaagaacagg?cttccctcta?tgccgcagtc?ctcgaagaca?tcgaagagac?gatggaagag??2580

gctgaagaag?gcatccagag?aaaaggtata?atcctgtccg?cccttaccag?gctcaaacag??2640

gtctgcaacc?atccggcgca?gtttttgaag?gataactctg?ctgtacccgg?caggtcagga??2700

aaacttgcaa?ggcttaccga?aatgctggat?gtaatcctgg?aaaatgggga?aaaagccctt??2760

gtgttcaccc?agtttgcgga?gatgggaaaa?atgctaaaag?aacacctgca?ggcaagtttt??2820

ggctgtgaag?tccttttcct?gcacggcggg?gtccccagaa?agcagaggga?tcggatgctt??2880

gagcgtttcc?aggagggaaa?agaatacctc?cctatctttg?tcctctccct?taaagctgga??2940

ggcacggggc?ttaaccttac?aggagcgaac?cacgttttcc?attttgaccg?ctggtggaac??3000

cctgctgttg?aaaaccaggc?tacggacagg?gctttccgta?taggccagac?gaaaaatgta??3060

gaggtgcata?agttcatctg?tgcgggtacg?cttgaagaaa?aaatcgatga?gattatcgag??3120

cgcaaagtgc?aggttgcaga?gaacgttgtc?ggaacaggtg?aaggttggct?gacagaactt??3180

tccaacgagg?aattgaagga?tattcttgct?ctccgagaag?aagcggtagg?tgaataa?????3237

<210>44

<211>1078

<212>PRT

＜213〉bite the acetate sarcina methanica

<400>44

Met?Ile?Ile?Leu?His?Ala?Gly?Arg?Val?Gly?Lys?Gln?Phe?Phe?Leu?Trp

1???????????????5???????????????????10??????????????????15

Gly?Glu?Ser?Pro?Ala?Glu?Asn?Glu?Thr?Pro?Pro?Val?Arg?Arg?Gly?Arg

20??????????????????25??????????????????30

Lys?Pro?Lys?Lys?Pro?Val?Ala?Lys?Pro?Tyr?Pro?Tyr?Asp?Ser?Gly?Val

35??????????????????40??????????????????45

Glu?Asn?Leu?Ser?Ser?Ala?Leu?Glu?Leu?Leu?Leu?Gly?Ser?Thr?Gly?Arg

50??????????????????55??????????????????60

Lys?Lys?Ala?Glu?Glu?Ile?Asn?Val?Trp?Ile?Pro?Thr?Ala?Gly?Trp?Asn

65??????????????????70??????????????????75??????????????????80

Pro?Ile?Pro?Ser?Ser?Pro?Leu?Val?Ala?Glu?Ile?Pro?Ala?Ser?Lys?Ala

85??????????????????90??????????????????95

Glu?Leu?Ser?Leu?Ala?Pro?Trp?Thr?Val?His?Ala?Tyr?Pro?Leu?Glu?Ala

100?????????????????105?????????????????110

Glu?Glu?Ala?Ile?Val?Leu?Leu?Cys?Ala?Cys?Met?Gly?Lys?Lys?Val?Leu

115?????????????????120?????????????????125

Ala?Pro?Gly?Ile?Ile?Ser?Gly?Asn?Asp?Leu?Leu?Trp?Trp?Ala?Asp?Ala

130?????????????????135?????????????????140

Leu?Lys?Phe?Ala?Gly?Ser?Leu?Val?Ala?Gly?Gln?Lys?Tyr?Leu?Pro?Gly

145?????????????????150?????????????????155?????????????????160

Val?Arg?Gly?Gly?Glu?Gly?Glu?Tyr?Lys?Ala?Phe?Trp?Glu?Pro?Val?Phe

165?????????????????170?????????????????175

Ser?Gly?Glu?Asp?Ala?Gly?Glu?Leu?Ala?Arg?Leu?Ala?Lys?Gln?Met?Pro

180?????????????????185?????????????????190

Pro?Ala?Ala?Lys?Ala?Leu?Ala?Leu?Glu?Thr?Ser?Ser?Val?Gln?Pro?Glu

195?????????????????200?????????????????205

Ile?Leu?Ala?Ala?Val?Ala?Ala?Arg?Gln?Phe?Ile?Glu?Glu?Ala?Leu?Asp

210?????????????????215?????????????????220

Trp?Ile?Val?Arg?Ser?Glu?Ile?Gly?Glu?Lys?Glu?Leu?Ala?Lys?Glu?Ala

225?????????????????230?????????????????235?????????????????240

Arg?Lys?Arg?Lys?Ser?Phe?Asp?Ser?Val?His?Asp?Ala?Trp?Val?Ser?Ala

245?????????????????250?????????????????255

Leu?Lys?Ser?Pro?Asp?Gly?Leu?Ile?His?Gly?Glu?Glu?Lys?Glu?Leu?Leu

260?????????????????265?????????????????270

Gln?Leu?Ala?Phe?Arg?Thr?Arg?Glu?Trp?Gln?Arg?Pro?Leu?Thr?Val?Leu

275?????????????????280?????????????????285

Thr?Thr?Ser?Pro?Phe?Arg?Phe?Cys?Phe?Arg?Leu?Glu?Glu?Pro?Ala?Ala

290?????????????????295?????????????????300

Glu?Glu?Glu?Leu?Glu?Glu?Thr?Glu?Glu?Ser?Glu?Ala?Gly?Lys?Met?Asp

305?????????????????310?????????????????315?????????????????320

Thr?Lys?Lys?Gly?Arg?Lys?Gly?Ile?Ala?Asp?Ile?Glu?Val?Pro?Glu?Glu

325?????????????????330?????????????????335

Leu?Trp?Tyr?Val?Arg?Tyr?Met?Leu?Gln?Ser?Tyr?Glu?Asp?Pro?Ser?Leu

340?????????????????345?????????????????350

Leu?Ile?Pro?Val?Lys?Glu?Ala?Trp?Lys?Pro?Lys?Lys?Gly?Ser?Pro?Leu

355?????????????????360?????????????????365

Lys?Arg?Tyr?Asp?Val?Lys?Asn?Ile?Arg?Gln?Phe?Leu?Leu?Ser?Ser?Leu

370?????????????????375?????????????????380

Gly?Gln?Ala?Ala?Gly?Ile?Ser?Ala?Gly?Ile?Ala?Ser?Ser?Leu?Glu?Ala

385?????????????????390?????????????????395?????????????????400

Pro?Asn?Pro?Ser?Gly?Tyr?Ser?Leu?Asp?Thr?Lys?Glu?Ala?Tyr?Arg?Phe

405?????????????????410?????????????????415

Leu?Thr?Glu?Ser?Ala?Ala?Asp?Leu?Ser?Gln?Ala?Gly?Phe?Gly?Leu?Leu

420?????????????????425?????????????????430

Leu?Pro?Gly?Trp?Trp?Thr?Arg?Lys?Gly?Thr?Lys?Thr?His?Leu?Lys?Ala

435?????????????????440?????????????????445

Gln?Ala?Asn?Val?Lys?Gly?Lys?Lys?Leu?Lys?Ala?Gly?Tyr?Gly?Leu?Thr

450?????????????????455?????????????????460

Leu?Asp?Lys?Ile?Val?Ser?Phe?Asp?Trp?Glu?Ile?Ala?Leu?Gly?Asp?Arg

465?????????????????470?????????????????475?????????????????480

Ala?Leu?Thr?Val?Arg?Glu?Leu?Gln?Ala?Leu?Ala?Lys?Leu?Lys?Ala?Pro

485?????????????????490?????????????????495

Leu?Val?Lys?Phe?Arg?Gly?Gln?Trp?Val?Glu?Val?Asn?Asp?Ala?Glu?Ile

500?????????????????505?????????????????510

Arg?Ala?Ala?Leu?Glu?Phe?Trp?Lys?Lys?Asn?Pro?His?Gly?Glu?Ala?Ser

515?????????????????520?????????????????525

Leu?Arg?Glu?Val?Leu?Lys?Leu?Ala?Val?Gly?Val?Ser?Glu?Lys?Ala?Asp

530?????????????????535?????????????????540

Gly?Val?Asp?Val?Glu?Gly?Leu?Asn?Ala?Ala?Gly?Trp?Ile?Glu?Glu?Leu

545?????????????????550?????????????????555?????????????????560

Ile?Arg?Arg?Leu?Lys?Asp?Lys?Thr?Gly?Phe?Glu?Glu?Leu?Pro?Ala?Pro

565?????????????????570?????????????????575

Asp?Gly?Phe?Ser?Gly?Thr?Leu?Arg?Pro?Tyr?Gln?Phe?Arg?Gly?Tyr?Ser

580?????????????????585?????????????????590

Trp?Leu?Ala?Phe?Leu?Arg?Gln?Trp?Gly?Ile?Gly?Ala?Cys?Leu?Ala?Asp

595?????????????????600?????????????????605

Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Thr?Leu?Ala?Leu?Ile?Gln?His

610?????????????????615?????????????????620

Asp?Leu?Glu?Gln?Val?Lys?Gly?Gln?Val?Glu?Glu?Lys?Val?Ile?Glu?Asn

625?????????????????630?????????????????635?????????????????640

Ala?Glu?Glu?Lys?Val?Glu?Gly?Leu?Lys?Ala?Ala?Lys?Pro?Val?Leu?Leu

645?????????????????650?????????????????655

Val?Cys?Pro?Thr?Ser?Val?Ile?Asn?Asn?Trp?Lys?Lys?Glu?Ala?Ala?Arg

660?????????????????665?????????????????670

Phe?Thr?Pro?Glu?Leu?Ser?Val?Met?Val?His?His?Gly?Thr?Ser?Arg?Lys

675?????????????????680?????????????????685

Lys?Glu?Glu?Glu?Phe?Lys?Lys?Glu?Ala?Thr?Asn?His?Ser?Ile?Val?Val

690?????????????????695?????????????????700

Ser?Ser?Tyr?Gly?Leu?Leu?Gln?Arg?Asp?Leu?Lys?Phe?Leu?Lys?Gly?Val

705?????????????????710?????????????????715?????????????????720

Ser?Trp?Ala?Gly?Val?Val?Leu?Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn?Pro

725?????????????????730?????????????????735

Glu?Thr?Lys?Gln?Ala?Lys?Ala?Ala?Arg?Ala?Leu?Glu?Ala?Asp?Tyr?Arg

740?????????????????745?????????????????750

Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Asn?Val?Gly?Asp?Leu?Trp

755?????????????????760?????????????????765

Ser?Ile?Met?Glu?Phe?Leu?Asn?Pro?Gly?Phe?Leu?Gly?Asn?Gln?Ala?Gly

770?????????????????775?????????????????780

Phe?Lys?Arg?Asn?Phe?Phe?Ile?Pro?Ile?Gln?Ala?Glu?Arg?Asp?Gln?Glu

785?????????????????790?????????????????795?????????????????800

Ala?Ala?Arg?Arg?Leu?Lys?Glu?Ile?Thr?Gly?Pro?Phe?Ile?Leu?Arg?Arg

805?????????????????810?????????????????815

Leu?Lys?Thr?Asp?Thr?Ser?Ile?Ile?Ser?Asp?Leu?Pro?Glu?Lys?Met?Glu

820?????????????????825?????????????????830

Met?Lys?Thr?Tyr?Cys?Thr?Leu?Thr?Lys?Glu?Gln?Ala?Ser?Leu?Tyr?Ala

835?????????????????840?????????????????845

Ala?Val?Leu?Glu?Asp?Ile?Glu?Glu?Thr?Met?Glu?Glu?Ala?Glu?Glu?Gly

850?????????????????855?????????????????860

Ile?Gln?Arg?Lys?Gly?Ile?Ile?Leu?Ser?Ala?Leu?Thr?Arg?Leu?Lys?Gln

865?????????????????870?????????????????875?????????????????880

Val?Cys?Asn?His?Pro?Ala?Gln?Phe?Leu?Lys?Asp?Asn?Ser?Ala?Val?Pro

885?????????????????890?????????????????895

Gly?Arg?Ser?Gly?Lys?Leu?Ala?Arg?Leu?Thr?Glu?Met?Leu?Asp?Val?Ile

900?????????????????905?????????????????910

Leu?Glu?Asn?Gly?Glu?Lys?Ala?Leu?Val?Phe?Thr?Gln?Phe?Ala?Glu?Met

915?????????????????920?????????????????925

Gly?Lys?Met?Leu?Lys?Glu?His?Leu?Gln?Ala?Ser?Phe?Gly?Cys?Glu?Val

930?????????????????935?????????????????940

Leu?Phe?Leu?His?Gly?Gly?Val?Pro?Arg?Lys?Gln?Arg?Asp?Arg?Met?Leu

945?????????????????950?????????????????955?????????????????960

Glu?Arg?Phe?Gln?Glu?Gly?Lys?Glu?Tyr?Leu?Pro?Ile?Phe?Val?Leu?Ser

965?????????????????970?????????????????975

Leu?Lys?Ala?Gly?Gly?Thr?Gly?Leu?Asn?Leu?Thr?Gly?Ala?Asn?His?Val

980?????????????????985?????????????????990

Phe?His?Phe?Asp?Arg?Trp?Trp?Asn??Pro?Ala?Val?Glu?Asn??Gln?Ala?Thr

995?????????????????1000?????????????????1005

Asp?Arg??Ala?Phe?Arg?Ile?Gly??Gln?Thr?Lys?Asn?Val??Glu?Val?His

1010?????????????????1015?????????????????1020

Lys?Phe??Ile?Cys?Ala?Gly?Thr??Leu?Glu?Glu?Lys?Ile??Asp?Glu?Ile

1025?????????????????1030?????????????????1035

Ile?Glu??Arg?Lys?Val?Gln?Val??Ala?Glu?Asn?Val?Val??Gly?Thr?Gly

1040?????????????????1045?????????????????1050

Glu?Gly??Trp?Leu?Thr?Glu?Leu??Ser?Asn?Glu?Glu?Leu??Lys?Asp?Ile

1055?????????????????1060?????????????????1065

Leu?Ala??Leu?Arg?Glu?Glu?Ala??Val?Gly?Glu

1070?????????????????1075

<210>45

<211>3147

<212>DNA

<213>Methanospirillum?hungatei

<400>45

gtgaccgcga?aacgaccagc?accaatccac?gataaagaag?aagagaccat?acccgatact?????60

tcgcttccgg?tctttcatgc?cctgatttac?ccggccgttg?aaggggtagc?gatatgtgcc????120

gaatatataa?ctgataaacc?tgcaccggtc?aggaaaaaag?gctacgcaaa?ggataaacct????180

ggcgaatatc?catattccct?ggatcatacc?gcccttaaaa?cgctcataga?gaactgtttt????240

ggagcatatg?atgacctgaa?ggctaccaga?tggattatct?atctccccgc?tgaagaaacg????300

gttcctcctt?cctctcagtt?ctcatcaaaa?aagaagccat?caccaaagga?gaaaaaactc????360

ccccttgttc?cgatgtatat?ccccgttctt?ctctgcccgt?atgaaacctt?ttttcaaatc????420

tggaaagccg?ctcagaatac?agataaaaat?tatattgctg?gcgattcctt?ccagtacatc????480

tccattctga?tggagagtac?cgtccggctc?atacaaaacg?gacggttcaa?accatctcta????540

gaacggacct?ttgccggata?tcatgccgta?tgggtacctg?ccctttctcc?tcaggatatg????600

gaatgggtat?cagatttttc?aagccggatg?ccaacggtct?gcaagtacgc?tatcccccgg????660

gtcgcaaaag?atccctacat?ttataaacct?gagaccagat?tagagaaatt?catcgttgag????720

atgatgcggg?tgatcatccg?tactgccctt?ggtggttata?cactgaaaga?agagacagat????780

cccttttatg?aaccctcaga?aaacgagatg?cagttcatga?ctgaccttct?cggggtaacc????840

gacccaataa?ggaacaaagg?atttgagaga?actttcttac?gggcgatgca?ggactggctg????900

accttctcaa?gttcaggacg?gtttgctccc?tttgagttct?gcatgatcat?aaaagatcca????960

ccagaaggac?agacagaacc?atgggatttc?actctcgcgg?tcagatcaga?ggcagaacca???1020

tctcttctca?tcccggcaga?aataatctgg?gaattgcctg?atcaccagag?cgggctcttc???1080

ccccaggcag?cctatctcaa?acatatcctc?cttgctggta?tcgggctctt?gacctcatca???1140

tcatcggcat?tatggcgtcc?cctgtccgga?tcgaaaccca?ccgggggaag?tatgaccctg????1200

aaagaggctg?caacgttctt?gggttcagac?ctcgcaagag?ccaggaggaa?gggagtaacg????1260

gtgctcctgc?cagactggtg?gactgatacg?acctatacac?cacgggttga?aatccatgca????1320

aggcggcggg?atcccaccca?tacgcagaca?cggataggac?tgcaggaact?cctttctttt????1380

gattaccgga?ttgcaatcgg?tgatgagtca?ttttcaccgg?atgagttctg?ggaaaaggta????1440

aaagaaaagg?ctccctttat?ctggctgggg?aaccggtgga?tatcctttca?tccggatgcg????1500

atacaacatg?ccctggattc?tttcagcagg?catcagagca?aaggagggga?tacaatagga????1560

gatctgctcc?ggctctccct?gaaaaaaatg?gaggattccg?cggtaccggt?atcgattcat????1620

gcaaaagatg?actgggttgc?ggatcttctg?gattttttca?ggaccgaaac?aaatcaggca????1680

gttccagtcc?caaagaaatt?taaagggata?ctcaggccat?accaggaaga?ggggttctcc????1740

ttcctttgtc?aatgtaccag?aaggggcttt?ggagcctgcc?ttgcagatga?catggggctt????1800

ggaaaaactc?cccagacact?tgcatggctg?gtctatctca?aggagaaaga?aaaacccacg????1860

actccgtccc?tccttatatg?cccgatgtcg?gttgttggga?actgggagcg?ggagatacag????1920

cggtttgcgc?catcactccg?ttcatgggtg?catcatggga?ctgaccgatg?caaaggcgat????1980

gattttgtga?gacatgtcgg?ttcatatgac?ctggtcctga?ccacctatca?tctggcagca????2040

cgggacgtag?accacctcaa?aaccgttccc?tggtctgcaa?tcattcttga?cgaggcacaa????2100

aatatcaaga?acctccatgc?aaaccagacc?gtagcagtca?aatctctcac?cggtgagaga????2160

cgggttgctc?tgaccggaac?cccggtggag?aaccggttac?tcgaactctg?gtctatcatg????2220

gactttttaa?atccaggata?ccttggttca?cagagtgcat?ttacaaaccg?ctattcccgc????2280

ccgattgagc?aggaaaaaaa?tacggaactg?atacaggaat?taaggtccct?catccgtccg????2340

ttcctgctca?ggcggatgaa?aacagacaag?catgttatcg?atgatcttcc?ggaaaagatg????2400

gagaaccggg?tatattgcac?cctcacaccc?gaacaggcaa?ccttatatca?ggctgttgtg????2460

cttgatatgg?caaagaacct?tgataaagtg?gagggtattg?ccaggaaagg?ggcaatcctt????2520

gctgcgatca?cacgactgaa?acagatctgt?aaccatccgg?gacgtgttgg?cagggataaa????2580

acaataaagg?ctgagcggtc?cgggaaggtg?agccggctgc?ttgagatgat?tgaggagatc????2640

acttccgaag?gggactcagc?actcatattc?agtcagtatg?caacatttgc?tgaggaactg????2700

gcagggatga?tagagaaaca?gggagatacg?cccgttcttc?tcctgaccgg?gtcaacacca????2760

cggaaaaaac?gggaacagat?gatagaggag?tttcaggcct?caaccacccc?gataatcttt????2820

gttatttctc?tgaaagccgg?gggaacgggt?ctgaacctga?cgaaagcgac?tcatgtgttt????2880

catgtagacc?ggtggtggaa?tccggcggtt?gaagaccagg?ctactgaccg?gacgtaccgg????2940

atcggacaaa?agagaaatgt?ccaagttcac?ctgatgataa?ccgccggaac?cctggaggaa????3000

cggatagatc?tgataaacca?ggagaaacgg?acgcttgcaa?aggaagtcct?tgcacagagt????3060

gatgagtatc?tgacaaatct?ctcaacaaaa?gaacttctgg?agattgtatc?acttcgtgac????3120

agtctctttc?gcggggagga?tgcatga????????????????????????????????????????3147

<210>46

<211>1048

<212>PRT

<213>Methanospirillum?hungatei

<400>46

Val?Thr?Ala?Lys?Arg?Pro?Ala?Pro?Ile?His?Asp?Lys?Glu?Glu?Glu?Thr

1???????????????5???????????????????10??????????????????15

Ile?Pro?Asp?Thr?Ser?Leu?Pro?Val?Phe?His?Ala?Leu?Ile?Tyr?Pro?Ala

20??????????????????25??????????????????30

Val?Glu?Gly?Val?Ala?Ile?Cys?Ala?Glu?Tyr?Ile?Thr?Asp?Lys?Pro?Ala

35??????????????????40??????????????????45

Pro?Val?Arg?Lys?Lys?Gly?Tyr?Ala?Lys?Asp?Lys?Pro?Gly?Glu?Tyr?Pro

50??????????????????55??????????????????60

Tyr?Ser?Leu?Asp?His?Thr?Ala?Leu?Lys?Thr?Leu?Ile?Glu?Asn?Cys?Phe

65??????????????????70??????????????????75??????????????????80

Gly?Ala?Tyr?Asp?Asp?Leu?Lys?Ala?Thr?Arg?Trp?Ile?Ile?Tyr?Leu?Pro

85??????????????????90??????????????????95

Ala?Glu?Glu?Thr?Val?Pro?Pro?Ser?Ser?Gln?Phe?Ser?Ser?Lys?Lys?Lys

100?????????????????105?????????????????110

Pro?Ser?Pro?Lys?Glu?Lys?Lys?Leu?Pro?Leu?Val?Pro?Met?Tyr?Ile?Pro

115?????????????????120?????????????????125

Val?Leu?Leu?Cys?Pro?Tyr?Glu?Thr?Phe?Phe?Gln?Ile?Trp?Lys?Ala?Ala

130?????????????????135?????????????????140

Gln?Asn?Thr?Asp?Lys?Asn?Tyr?Ile?Ala?Gly?Asp?Ser?Phe?Gln?Tyr?Ile

145?????????????????150?????????????????155?????????????????160

Ser?Ile?Leu?Met?Glu?Ser?Thr?Val?Arg?Leu?Ile?Gln?Asn?Gly?Arg?Phe

165?????????????????170?????????????????175

Lys?Pro?Ser?Leu?Glu?Arg?Thr?Phe?Ala?Gly?Tyr?His?Ala?Val?Trp?Val

180?????????????????185?????????????????190

Pro?Ala?Leu?Ser?Pro?Gln?Asp?Met?Glu?Trp?Val?Ser?Asp?Phe?Ser?Ser

195?????????????????200?????????????????205

Arg?Met?Pro?Thr?Val?Cys?Lys?Tyr?Ala?Ile?Pro?Arg?Val?Ala?Lys?Asp

210?????????????????215?????????????????220

Pro?Tyr?Ile?Tyr?Lys?Pro?Glu?Thr?Arg?Leu?Glu?Lys?Phe?Ile?Val?Glu

225?????????????????230?????????????????235?????????????????240

Met?Met?Arg?Val?Ile?Ile?Arg?Thr?Ala?Leu?Gly?Gly?Tyr?Thr?Leu?Lys

245?????????????????250?????????????????255

Glu?Glu?Thr?Asp?Pro?Phe?Tyr?Glu?Pro?Ser?Glu?Asn?Glu?Met?Gln?Phe

260?????????????????265?????????????????270

Met?Thr?Asp?Leu?Leu?Gly?Val?Thr?Asp?Pro?Ile?Arg?Asn?Lys?Gly?Phe

275?????????????????280?????????????????285

Glu?Arg?Thr?Phe?Leu?Arg?Ala?Met?Gln?Asp?Trp?Leu?Thr?Phe?Ser?Ser

290?????????????????295?????????????????300

Ser?Gly?Arg?Phe?Ala?Pro?Phe?Glu?Phe?Cys?Met?Ile?Ile?Lys?Asp?Pro

305?????????????????310?????????????????315?????????????????320

Pro?Glu?Gly?Gln?Thr?Glu?Pro?Trp?Asp?Phe?Thr?Leu?Ala?Val?Arg?Ser

325?????????????????330?????????????????335

Glu?Ala?Glu?Pro?Ser?Leu?Leu?Ile?Pro?Ala?Glu?Ile?Ile?Trp?Glu?Leu

340?????????????????345?????????????????350

Pro?Asp?His?Gln?Ser?Gly?Leu?Phe?Pro?Gln?Ala?Ala?Tyr?Leu?Lys?His

355?????????????????360?????????????????365

Ile?Leu?Leu?Ala?Gly?Ile?Gly?Leu?Leu?Thr?Ser?Ser?Ser?Ser?Ala?Leu

370?????????????????375?????????????????380

Trp?Arg?Pro?Leu?Ser?Gly?Ser?Lys?Pro?Thr?Gly?Gly?Ser?Met?Thr?Leu

385?????????????????390?????????????????395?????????????????400

Lys?Glu?Ala?Ala?Thr?Phe?Leu?Gly?Ser?Asp?Leu?Ala?Arg?Ala?Arg?Arg

405?????????????????410?????????????????415

Lys?Gly?Val?Thr?Val?Leu?Leu?Pro?Asp?Trp?Trp?Thr?Asp?Thr?Thr?Tyr

420?????????????????425?????????????????430

Thr?Pro?Arg?Val?Glu?Ile?His?Ala?Arg?Arg?Arg?Asp?Pro?Thr?His?Thr

435?????????????????440?????????????????445

Gln?Thr?Arg?Ile?Gly?Leu?Gln?Glu?Leu?Leu?Ser?Phe?Asp?Tyr?Arg?Ile

450?????????????????455?????????????????460

Ala?Ile?Gly?Asp?Glu?Ser?Phe?Ser?Pro?Asp?Glu?Phe?Trp?Glu?Lys?Val

465?????????????????470?????????????????475?????????????????480

Lys?Glu?Lys?Ala?Pro?Phe?Ile?Trp?Leu?Gly?Asn?Arg?Trp?Ile?Ser?Phe

485?????????????????490?????????????????495

His?Pro?Asp?Ala?Ile?Gln?His?Ala?Leu?Asp?Ser?Phe?Ser?Arg?His?Gln

500?????????????????505?????????????????510

Ser?Lys?Gly?Gly?Asp?Thr?Ile?Gly?Asp?Leu?Leu?Arg?Leu?Ser?Leu?Lys

515?????????????????520?????????????????525

Lys?Met?Glu?Asp?Ser?Ala?Val?Pro?Val?Ser?Ile?His?Ala?Lys?Asp?Asp

530?????????????????535?????????????????540

Trp?Val?Ala?Asp?Leu?Leu?Asp?Phe?Phe?Arg?Thr?Glu?Thr?Asn?Gln?Ala

545?????????????????550?????????????????555?????????????????560

Val?Pro?Val?Pro?Lys?Lys?Phe?Lys?Gly?Ile?Leu?Arg?Pro?Tyr?Gln?Glu

565?????????????????570?????????????????575

Glu?Gly?Phe?Ser?Phe?Leu?Cys?Gln?Cys?Thr?Arg?Arg?Gly?Phe?Gly?Ala

580?????????????????585?????????????????590

Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Pro?Gln?Thr?Leu?Ala

595?????????????????600?????????????????605

Trp?Leu?Val?Tyr?Leu?Lys?Glu?Lys?Glu?Lys?Pro?Thr?Thr?Pro?Ser?Leu

610?????????????????615?????????????????620

Leu?Ile?Cys?Pro?Met?Ser?Val?Val?Gly?Asn?Trp?Glu?Arg?Glu?Ile?Gln

625?????????????????630?????????????????635?????????????????640

Arg?Phe?Ala?Pro?Ser?Leu?Arg?Ser?Trp?Val?His?His?Gly?Thr?Asp?Arg

645?????????????????650?????????????????655

Cys?Lys?Gly?Asp?Asp?Phe?Val?Arg?His?Val?Gly?Ser?Tyr?Asp?Leu?Val

660?????????????????665?????????????????670

Leu?Thr?Thr?Tyr?His?Leu?Ala?Ala?Arg?Asp?Val?Asp?His?Leu?Lys?Thr

675?????????????????680?????????????????685

Val?Pro?Trp?Ser?Ala?Ile?Ile?Leu?Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn

690?????????????????695?????????????????700

Leu?His?Ala?Asn?Gln?Thr?Val?Ala?Val?Lys?Ser?Leu?Thr?Gly?Glu?Arg

705?????????????????710?????????????????715?????????????????720

Arg?Val?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu?Leu?Glu?Leu

725?????????????????730?????????????????735

Trp?Ser?Ile?Met?Asp?Phe?Leu?Asn?Pro?Gly?Tyr?Leu?Gly?Ser?Gln?Ser

740?????????????????745?????????????????750

Ala?Phe?Thr?Asn?Arg?Tyr?Ser?Arg?Pro?Ile?Glu?Gln?Glu?Lys?Asn?Thr

755?????????????????760?????????????????765

Glu?Leu?Ile?Gln?Glu?Leu?Arg?Ser?Leu?Ile?Arg?Pro?Phe?Leu?Leu?Arg

770?????????????????775?????????????????780

Arg?Met?Lys?Thr?Asp?Lys?His?Val?Ile?Asp?Asp?Leu?Pro?Glu?Lys?Met

785?????????????????790?????????????????795?????????????????800

Glu?Asn?Arg?Val?Tyr?Cys?Thr?Leu?Thr?Pro?Glu?Gln?Ala?Thr?Leu?Tyr

805?????????????????810?????????????????815

Gln?Ala?Val?Val?Leu?Asp?Met?Ala?Lys?Asn?Leu?Asp?Lys?Val?Glu?Gly

820?????????????????825?????????????????830

Ile?Ala?Arg?Lys?Gly?Ala?Ile?Leu?Ala?Ala?Ile?Thr?Arg?Leu?Lys?Gln

835?????????????????840?????????????????845

Ile?Cys?Asn?His?Pro?Gly?Arg?Val?Gly?Arg?Asp?Lys?Thr?Ile?Lys?Ala

850?????????????????855?????????????????860

Glu?Arg?Ser?Gly?Lys?Val?Ser?Arg?Leu?Leu?Glu?Met?Ile?Glu?Glu?Ile

865?????????????????870?????????????????875?????????????????880

Thr?Ser?Glu?Gly?Asp?Ser?Ala?Leu?Ile?Phe?Ser?Gln?Tyr?Ala?Thr?Phe

885?????????????????890?????????????????895

Ala?Glu?Glu?Leu?Ala?Gly?Met?Ile?Glu?Lys?Gln?Gly?Asp?Thr?Pro?Val

900?????????????????905?????????????????910

Leu?Leu?Leu?Thr?Gly?Ser?Thr?Pro?Arg?Lys?Lys?Arg?Glu?Gln?Met?Ile

915?????????????????920?????????????????925

Glu?Glu?Phe?Gln?Ala?Ser?Thr?Thr?Pro?Ile?Ile?Phe?Val?Ile?Ser?Leu

930?????????????????935?????????????????940

Lys?Ala?Gly?Gly?Thr?Gly?Leu?Asn?Leu?Thr?Lys?Ala?Thr?His?Val?Phe

945?????????????????950?????????????????955?????????????????960

His?Val?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asp?Gln?Ala?Thr?Asp

965?????????????????970?????????????????975

Arg?Thr?Tyr?Arg?Ile?Gly?Gln?Lys?Arg?Asn?Val?Gln?Val?His?Leu?Met

980?????????????????985?????????????????990

Ile?Thr?Ala?Gly?Thr?Leu?Glu?Glu??Arg?Ile?Asp?Leu?Ile??Asn?Gln?Glu

995?????????????????1000?????????????????1005

Lys?Arg??Thr?Leu?Ala?Lys?Glu??Val?Leu?Ala?Gln?Ser??Asp?Glu?Tyr

1010?????????????????1015?????????????????1020

Leu?Thr??Asn?Leu?Ser?Thr?Lys??Glu?Leu?Leu?Glu?Ile??Val?Ser?Leu

1025?????????????????1030?????????????????1035

Arg?Asp??Ser?Leu?Phe?Arg?Gly??Glu?Asp?Ala

1040?????????????????1045

<210>47

<211>3270

<212>DNA

＜213〉Ma Shi sarcina methanica

<400>47

atgataattc?ttcatgcagg?aagagttgga?aaacagttct?tcttatgggg?tgaaagcccg?????60

gcagaaaatg?aaactccggt?tgttcggcgc?gggagaaagc?ctaaaacccc?tatcgtaaaa????120

ccttaccctt?acgattcggg?ctttgaaaac?ctgtcttctg?cccttgagct?gctgctgggc????180

agtactgacc?ggaaaaaggc?ggagaaaatc?aacgtctgga?ccccaactat?cggagggaat?????240

cctgtccctt?ccagccctct?tgttgctgaa?atttcggatt?cgaaagcaga?acctgcactg?????300

gctccctgta?ctgttcacgc?atatcctctg?gaagctgaag?aagctattgt?tctcctctgc?????360

acctgtatgg?aaaaaaaggt?tctggctccc?ggtatcatct?cgggaaatga?ccttctctgg?????420

tgggcagatg?ccctgaaatt?tgcaggctcg?ctggtagcag?ggcagaaata?tttgcctggc?????480

gtcaggggcg?gggaaggaga?gtacagggct?ttctgggaac?ccgtattttc?cggcgaagat?????540

gccggaaagc?tggcaaaact?tgcaaagcaa?atgcctcctg?ctgcaagggc?tcttgctcct?????600

gaagcctctt?ccatgccgcc?ggaaatgcct?gctgctttag?cggcaaagca?gtttattgaa?????660

gactctctcg?actggatagt?ccggtccgag?atcggggaaa?aaaagcttgc?aaaagagacg?????720

cgcaaaagaa?aatcctttga?tagcgtccat?gatgcctggg?tttctgctct?tagaagccct?????780

gaagggctga?tctatggaga?cgaaaacgaa?cttctgcagc?ttgcggcccg?gacccgcgaa?????840

tggcagcgcc?cactcaccat?ccttaccact?tctcctttca?ggttctgttt?ccgtcttgaa?????900

gaaccggctt?tagaagaaga?gatcgaagaa?actgaagaaa?ccgaagaaat?agaagaaaat?????960

gaagccggga?aaagagatac?taaaaaaggc?agggaaggga?tagctgatat?agaagttccc????1020

gaagggctct?ggtacgtccg?ttatatgctt?cagtcctacg?aagacccgag?ccttctgatc????1080

cctgtaaaag?aagcctggaa?gccaaaaaaa?ggcagcccgt?tgaaaaaata?cgatgtgaaa????1140

aacattcgcc?aattcctgtt?atcttccctt?ggacaggctt?ccagtataag?tgcaggaatt????1200

gcttcgagtc?ttgaagctcc?caacccatct?ggatattccc?ttgatactaa?agaggcttac????1260

cgctttctga?ctgaaagtgc?agcgaattta?agtcaggccg?gtttcggggt?acttctccct????1320

ggctggtgga?cccgtaaagg?tacaaagaca?cacttaaaag?cccaggctaa?tgttaagggc????1380

aagaagaagt?tgcaggccgg?atacgggctt?acactcgatg?aaatcgtcag?ctttgactgg????1440

gaaatcgccc?ttggagacag?ggtactgaca?gtcagagaac?tgcaggctct?tgcaaagctt????1500

aaagctccgc?ttgtgaaatt?ccgcgggcag?tgggttgagg?taaacgatgc?ggaaatcagg????1560

gctgcccttg?agttctggaa?gaaaaatccc?aacggtgaag?caagtctgcg?tgaagttcta????1620

aaactggcag?tgggagtttc?cgaaaaagcc?gatggtgtga?acgttgaagg?gctcaatgca????1680

accggatgga?ttggagaatt?aatcagccgc?ttaaaagaca?aaaccgggtt?tgaagaactt????1740

cctgctccca?acggcttttc?aggcaccctt?cggccatatc?agttcagagg?ttactcctgg????1800

ctggcttttc?tgaggcagtg?gggtatagga?gcctgccttg?cagacgatat?ggggcttggt????1860

aaaaccgtcc?agactcttgc?tcttattcag?cacgatctgg?aacaggctaa?agagaaagct????1920

gaagaaaaga?ttgaagaacc?ggctgaagaa?aagattgaag?aaaaagttga?cggacgtaag????1980

gccccaaaac?ctgttcttct?ggtttgtcct?acctctgtta?tcaacaactg?gaaaaaagag????2040

gcttcccgct?ttacgccaga?actttcggta?atggtccacc?acgggaccag?ccggaaaaag????2100

gaagaggaat?tcaagaagga?agccatgaat?catgctattg?tcatctcaag?ctatggcctt????2160

gtgcagcggg?atcttaaatt?tttaaaagag?gttcattggg?caggagttgt?acttgacgaa????2220

gcccagaaca?tcaaaaaccc?ggaaaccaaa?caggcaaagg?cagccagggc?tcttgaatcc????2280

gattaccgct?tagctcttac?agggactccg?gttgaaaata?acgtgggaga?cctctggtcc????2340

ataatggagt?ttttaaaccc?cggcttcctc?ggaagtcagg?cgggtttcaa?gcggaatttc????2400

tttatcccca?ttcaggcaga?aagggatcag?gaggctgcaa?ggaggctgaa?agaaattaca????2460

ggtcccttca?tccttcgccg?tttgaagact?gacacttcga?ttatctccga?cctgccggaa????2520

aaaatggaga?tgaagaccta?ttgtacgctg?acaaaagaac?aggcctccct?ctatgctgca????2580

gtccttgaag?acatcagaga?agcgattgaa?ggagccgaag?aaggcatcca?gaggaaaggt????2640

ataatcctgt?ctgccctttc?caggctcaag?caggtctgca?accaccctgc?gcagtttttg????2700

aaggacaact?ccactatccc?cggcaggtcc?ggaaaactcg?caaggcttac?cgaaatgctg????2760

gatgtagtcc?tggaaaacgg?ggaaaaagcc?cttgttttta?cccagtttgc?ggagatgggc????2820

aaaatggtga?aagaacacct?gcaagcaagc?tttggctgtg?aagtcctttt?cctgcacggc??2880

ggggtcccca?ggaagcagag?agaccggatg?cttgagaggt?tccaggaagg?aaaagaatac??2940

ctccctattt?ttgtcctctc?ccttaaagcc?ggcggcacgg?ggcttaacct?cacaggggca??3000

aaccacgttt?tccactttga?tcgctggtgg?aacccggctg?ttgaaaacca?ggctacagac??3060

agggcattcc?gtataggcca?gaagaaaaac?gttgaggtcc?ataaattcat?ctgcgcaggt??3120

acgcttgaag?aaaaaatcga?tgagattatc?gaacgcaaag?tgcaggtcgc?agagaacgtt??3180

gttgggacag?gtgaagactg?gctgacagag?ctttccaacg?atgaactgaa?ggatattctt??3240

gctcttagag?aagaagcggt?aggtgaataa???????????????????????????????????3270

<210>48

<211>1089

<212>PRT

＜213〉Ma Shi sarcina methanica

<400>48

Met?Ile?Ile?Leu?His?Ala?Gly?Arg?Val?Gly?Lys?Gln?Phe?Phe?Leu?Trp

1???????????????5???????????????????10??????????????????15

Gly?Glu?Ser?Pro?Ala?Glu?Asn?Glu?Thr?Pro?Val?Val?Arg?Arg?Gly?Arg

20??????????????????25??????????????????30

Lys?Pro?Lys?Thr?Pro?Ile?Val?Lys?Pro?Tyr?Pro?Tyr?Asp?Ser?Gly?Phe

35??????????????????40??????????????????45

Glu?Asn?Leu?Ser?Ser?Ala?Leu?Glu?Leu?Leu?Leu?Gly?Ser?Thr?Asp?Arg

50??????????????????55??????????????????60

Lys?Lys?Ala?Glu?Lys?Ile?Asn?Val?Trp?Thr?Pro?Thr?Ile?Gly?Gly?Asn

65??????????????????70??????????????????75??????????????????80

Pro?Val?Pro?Ser?Ser?Pro?Leu?Val?Ala?Glu?Ile?Ser?Asp?Ser?Lys?Ala

85??????????????????90??????????????????95

Glu?Pro?Ala?Leu?Ala?Pro?Cys?Thr?Val?His?Ala?Tyr?Pro?Leu?Glu?Ala

100?????????????????105?????????????????110

Glu?Glu?Ala?Ile?Val?Leu?Leu?Cys?Thr?Cys?Met?Glu?Lys?Lys?Val?Leu

115?????????????????120?????????????????125

Ala?Pro?Gly?Ile?Ile?Ser?Gly?Asn?Asp?Leu?Leu?Trp?Trp?Ala?Asp?Ala

130?????????????????135?????????????????140

Leu?Lys?Phe?Ala?Gly?Ser?Leu?Val?Ala?Gly?Gln?Lys?Tyr?Leu?Pro?Gly

145?????????????????150?????????????????155?????????????????160

Val?Arg?Gly?Gly?Glu?Gly?Glu?Tyr?Arg?Ala?Phe?Trp?Glu?Pro?Val?Phe

165?????????????????170?????????????????175

Ser?Gly?Glu?Asp?Ala?Gly?Lys?Leu?Ala?Lys?Leu?Ala?Lys?Gln?Met?Pro

180?????????????????185?????????????????190

Pro?Ala?Ala?Arg?Ala?Leu?Ala?Pro?Glu?Ala?Ser?Ser?Met?Pro?Pro?Glu

195?????????????????200?????????????????205

Met?Pro?Ala?Ala?Leu?Ala?Ala?Lys?Gln?Phe?Ile?Glu?Asp?Ser?Leu?Asp

210?????????????????215?????????????????220

Trp?Ile?Val?Arg?Ser?Glu?Ile?Gly?Glu?Lys?Lys?Leu?Ala?Lys?Glu?Thr

225?????????????????230?????????????????235?????????????????240

Arg?Lys?Arg?Lys?Ser?Phe?Asp?Ser?Val?His?Asp?Ala?Trp?Val?Ser?Ala

245?????????????????250?????????????????255

Leu?Arg?Ser?Pro?Glu?Gly?Leu?Ile?Tyr?Gly?Asp?Glu?Asn?Glu?Leu?Leu

260?????????????????265?????????????????270

Gln?Leu?Ala?Ala?Arg?Thr?Arg?Glu?Trp?Gln?Arg?Pro?Leu?Thr?Ile?Leu

275?????????????????280?????????????????285

Thr?Thr?Ser?Pro?Phe?Arg?Phe?Cys?Phe?Arg?Leu?Glu?Glu?Pro?Ala?Leu

290?????????????????295?????????????????300

Glu?Glu?Glu?Ile?Glu?Glu?Thr?Glu?Glu?Thr?Glu?Glu?Ile?Glu?Glu?Asn

305?????????????????310?????????????????315?????????????????320

Glu?Ala?Gly?Lys?Arg?Asp?Thr?Lys?Lys?Gly?Arg?Glu?Gly?Ile?Ala?Asp

325?????????????????330?????????????????335

Ile?Glu?Val?Pro?Glu?Gly?Leu?Trp?Tyr?Val?Arg?Tyr?Met?Leu?Gln?Ser

340?????????????????345?????????????????350

Tyr?Glu?Asp?Pro?Ser?Leu?Leu?Ile?Pro?Val?Lys?Glu?Ala?Trp?Lys?Pro

355?????????????????360?????????????????365

Lys?Lys?Gly?Ser?Pro?Leu?Lys?Lys?Tyr?Asp?Val?Lys?Asn?Ile?Arg?Gln

370?????????????????375?????????????????380

Phe?Leu?Leu?Ser?Ser?Leu?Gly?Gln?Ala?Ser?SerIle?Ser?Ala?Gly?Ile

385?????????????????390?????????????????395?????????????????400

Ala?Ser?Ser?Leu?Glu?Ala?Pro?Asn?Pro?Ser?Gly?Tyr?Ser?Leu?Asp?Thr

405?????????????????410?????????????????415

Lys?Glu?Ala?Tyr?Arg?Phe?Leu?Thr?Glu?Ser?Ala?Ala?Asn?Leu?Ser?Gln

420?????????????????425?????????????????430

Ala?Gly?Phe?Gly?Val?Leu?Leu?Pro?Gly?Trp?Trp?Thr?Arg?Lys?Gly?Thr

435?????????????????440?????????????????445

Lys?Thr?His?Leu?Lys?Ala?Gln?Ala?Asn?Val?Lys?Gly?Lys?Lys?Lys?Leu

450?????????????????455?????????????????460

Gln?Ala?Gly?Tyr?Gly?Leu?Thr?Leu?Asp?Glu?Ile?Val?Ser?Phe?Asp?Trp

465?????????????????470?????????????????475?????????????????480

Glu?Ile?Ala?Leu?Gly?Asp?Arg?Val?Leu?Thr?Val?Arg?Glu?Leu?Gln?Ala

485?????????????????490?????????????????495

Leu?Ala?Lys?Leu?Lys?Ala?Pro?Leu?Val?Lys?Phe?Arg?Gly?Gln?Trp?Val

500?????????????????505?????????????????510

Glu?Val?Asn?Asp?Ala?Glu?Ile?Arg?Ala?Ala?Leu?Glu?Phe?Trp?Lys?Lys

515?????????????????520?????????????????525

Asn?Pro?Asn?Gly?Glu?Ala?Ser?Leu?Arg?Glu?Val?Leu?Lys?Leu?Ala?Val

530?????????????????535?????????????????540

Gly?Val?Ser?Glu?Lys?Ala?Asp?Gly?Val?Asn?Val?Glu?Gly?Leu?Asn?Ala

545?????????????????550?????????????????555?????????????????560

Thr?Gly?Trp?Ile?Gly?Glu?Leu?Ile?Ser?Arg?Leu?Lys?Asp?Lys?Thr?Gly

565?????????????????570?????????????????575

Phe?Glu?Glu?Leu?Pro?Ala?Pro?Asn?Gly?Phe?Ser?Gly?Thr?Leu?Arg?Pro

580?????????????????585?????????????????590

Tyr?Gln?Phe?Arg?Gly?Tyr?Ser?Trp?Leu?Ala?Phe?Leu?Arg?Gln?Trp?Gly

595?????????????????600?????????????????605

Ile?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Val?Gln

610?????????????????615?????????????????620

Thr?Leu?Ala?Leu?Ile?Gln?His?Asp?Leu?Glu?Gln?Ala?Lys?Glu?Lys?Ala

625?????????????????630?????????????????635?????????????????640

Glu?Glu?Lys?Ile?Glu?Glu?Pro?Ala?Glu?Glu?Lys?Ile?Glu?Glu?Lys?Val

645?????????????????650?????????????????655

Asp?Gly?Arg?Lys?Ala?Pro?Lys?Pro?Val?Leu?Leu?Val?Cys?Pro?Thr?Ser

660?????????????????665?????????????????670

Val?Ile?Asn?Asn?Trp?Lys?Lys?Glu?Ala?Ser?Arg?Phe?Thr?Pro?Glu?Leu

675?????????????????680?????????????????685

Ser?Val?Met?Val?His?His?Gly?Thr?Ser?Arg?Lys?Lys?Glu?Glu?Glu?Phe

690?????????????????695?????????????????700

Lys?Lys?Glu?Ala?Met?Asn?His?Ala?Ile?Val?Ile?Ser?Ser?Tyr?Gly?Leu

705?????????????????710?????????????????715?????????????????720

Val?Gln?Arg?Asp?Leu?Lys?Phe?Leu?Lys?Glu?Val?His?Trp?Ala?Gly?Val

725?????????????????730?????????????????735

Val?Leu?Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn?Pro?Glu?Thr?Lys?Gln?Ala

740?????????????????745?????????????????750

Lys?Ala?Ala?Arg?Ala?Leu?Glu?Ser?Asp?Tyr?Arg?Leu?Ala?Leu?Thr?Gly

755?????????????????760?????????????????765

Thr?Pro?Val?Glu?Asn?Asn?Val?Gly?Asp?Leu?Trp?Ser?Ile?Met?Glu?Phe

770?????????????????775?????????????????780

Leu?Asn?Pro?Gly?Phe?Leu?Gly?Ser?Gln?Ala?Gly?Phe?Lys?Arg?Asn?Phe

785?????????????????790?????????????????795?????????????????800

Phe?Ile?Pro?Ile?Gln?Ala?Glu?Arg?Asp?Gln?Glu?Ala?Ala?Arg?Arg?Leu

805?????????????????810?????????????????815

Lys?Glu?Ile?Thr?Gly?Pro?Phe?Ile?Leu?Arg?Arg?Leu?Lys?Thr?Asp?Thr

820?????????????????825?????????????????830

Ser?Ile?Ile?Ser?Asp?Leu?Pro?Glu?Lys?Met?Glu?Met?Lys?Thr?Tyr?Cys

835?????????????????840?????????????????845

Thr?Leu?Thr?Lys?Glu?Gln?Ala?Ser?Leu?Tyr?Ala?Ala?Val?Leu?Glu?Asp

850?????????????????855?????????????????860

Ile?Arg?Glu?Ala?Ile?Glu?Gly?Ala?Glu?Glu?Gly?Ile?Gln?Arg?Lys?Gly

865?????????????????870?????????????????875?????????????????880

Ile?Ile?Leu?Ser?Ala?Leu?Ser?Arg?Leu?Lys?Gln?Val?Cys?Asn?His?Pro

885?????????????????890?????????????????895

Ala?Gln?Phe?Leu?Lys?Asp?Asn?Ser?Thr?Ile?Pro?Gly?Arg?Ser?Gly?Lys

900?????????????????905?????????????????910

Leu?Ala?Arg?Leu?Thr?Glu?Met?Leu?Asp?Val?Val?Leu?Glu?Asn?Gly?Glu

915?????????????????920?????????????????925

Lys?Ala?Leu?Val?Phe?Thr?Gln?Phe?Ala?Glu?Met?Gly?Lys?Met?Val?Lys

930?????????????????935?????????????????940

Glu?His?Leu?Gln?Ala?Ser?Phe?Gly?Cys?Glu?Val?Leu?Phe?Leu?His?Gly

945?????????????????950?????????????????955?????????????????960

Gly?Val?Pro?Arg?Lys?Gln?Arg?Asp?Arg?Met?Leu?Glu?Arg?Phe?Gln?Glu

965?????????????????970?????????????????975

Gly?Lys?Glu?Tyr?Leu?Pro?Ile?Phe?Val?Leu?Ser?Leu?Lys?Ala?Gly?Gly

980?????????????????985?????????????????990

Thr?Gly?Leu?Asn?Leu?Thr?Gly?Ala??Asn?His?Val?Phe?His??Phe?Asp?Arg

995?????????????????1000?????????????????1005

Trp?Trp??Asn?Pro?Ala?Val?Glu??Asn?Gln?Ala?Thr?Asp??Arg?Ala?Phe

1010?????????????????1015?????????????????1020

Arg?Ile??Gly?Gln?Lys?Lys?Asn??Val?Glu?Val?His?Lys??Phe?Ile?Cys

1025?????????????????1030?????????????????1035

Ala?Gly??Thr?Leu?Glu?Glu?Lys??Ile?Asp?Glu?Ile?Ile??Glu?Arg?Lys

1040?????????????????1045?????????????????1050

Val?Gln??Val?Ala?Glu?Asn?Val??Val?Gly?Thr?Gly?Glu??Asp?Trp?Leu

1055?????????????????1060?????????????????1065

Thr?Glu??Leu?Ser?Asn?Asp?Glu??Leu?Lys?Asp?Ile?Leu??Ala?Leu?Arg

1070?????????????????1075?????????????????1080

Glu?Glu??Ala?Val?Gly?Glu

1085

<210>49

<211>3042

<212>DNA

＜213〉Mycobacterium bovis

<400>49

atgctggttt?tgcacggctt?ctggtccaac?tccggcggga?tgcggctgtg?ggcggaggac????60

tccgatctgc?tggtgaagag?cccgagtcag?gcgctgcgct?ccgcgcggcc?acacccgttc????120

gcggcgcccg?ctgacctgat?cgccggcata?catccgggca?aacccgcaac?cgccgttttg????180

ctgttgccgt?cgttgcgatc?ggcgccgctg?gactcgccgg?agctgatccg?gctcgccccg????240

cgcccggccg?cgcgaaccga?tccgatgctg?ttggcgtgga?cggtaccggt?ggtggacctg????300

gaccccaccg?cggcgttggc?cgccttcgac?cagcccgccc?ccgacgtccg?ctacggcgcg????360

tccgtcgact?acctggccga?gctggccgtt?ttcgcgcgcg?agttggtcga?gcgtggtcgc????420

gtgctgcccc?agctgcgccg?cgacacccac?ggcgcggccg?cctgctggcg?tccggtgttg????480

cagggacgcg?acgtggtcgc?gatgacctcg?ctggtctcgg?cgatgccgcc?ggtctgccgc????540

gccgaagttg?gtgggcacga?cccgcacgaa?ctggcaacct?cggctctgga?cgcgatggtc????600

gacgccgccg?tgcgcgcggc?gctgtcaccg?atggacctgc?tgcccccgcg?acggggtcgc????660

tccaaacggc?atcgggccgt?ggaggcttgg?ctgaccgcgt?tgacctgccc?ggacggccgg????720

ttcgacgcgg?agcccgacga?actcgacgcg?ctggccgagg?cgttgcggcc?atgggacgac????780

gtcggtatcg?gcaccgtcgg?cccggcgcgg?gcgacgtttc?ggctgtccga?agtcgagacc????840

gaaaacgagg?agacgcccgc?gggctcgttg?tggaggctgg?agttcttatt?gcagtcgacg????900

caggacccca?gcctgctggt?ccccgccgag?caggcatgga?acgacgacgg?cagcctgcgc????960

cgctggctgg?accggccgca?ggagctgctg?ctgaccgaac?tgggccgggc?ctctcggatt???1020

ttccccgagc?tcgtcccggc?gctgcgcacc?gcgtgcccgt?ccgggcttga?gctcgacgcc???1080

gacggcgcct?accgattcct?gtcgggtacg?gccgcggtgc?tcgacgaggc?tgggtttggc???1140

gtgctgctgc?cgtcctggtg?ggaccgccgc?cgcaagctgg?gcttggtcct?gtccgcatat???1200

accccggtcg?acggcgtggt?gggcaaggcc?agcaagttcg?gccgcgagca?gctcgtcgag???1260

ttccgctggg?agctggccgt?gggcgacgat?ccgctcagcg?aggaggagat?cgcggcgctg???1320

accgaaacca?agtccccgct?gatccggctg?cgtggccagt?gggtggcgct?cgataccgaa???1380

cagctgcgcc?gcgggctgga?gtttttggag?cgtaagccaa?ccggccgcaa?gaccaccgcc????1440

gagatcctcg?cgctggccgc?cagccacccc?gacgacgtgg?acaccccgct?cgaggtcacc????1500

gccgtacgcg?ccgacggctg?gctcggggac?ctgctcgccg?gggccgccgc?ggcgtcgctg????1560

cagccgttgg?acccgcccga?cggattcacc?gcgacgctgc?gtccctacca?gcagcgcggt????1620

ctggcgtggc?tggcgttttt?gtcctcgctc?ggtttgggca?gctgcctggc?cgacgacatg????1680

ggcctgggca?agacggtgca?gctattggcc?ctggaaacct?tggaatccgt?tcagcgccac????1740

caggatcgcg?gcgtcggacc?cacactgcta?ctgtgcccga?tgtcgttggt?gggcaactgg????1800

cagcaggaag?cggccaggtt?tgcacccaac?ctgcgggtgt?acgcccacca?cgggggcgcc????1860

cggctgcacg?gcgaggcgtt?gcgcgaccac?ctcgagcgca?ccgacctggt?cgtgagcacc????1920

tataccaccg?ccacccgcga?catcgacgag?ctgtcggaat?acgaatggaa?ccgggtggtg????1980

ctggacgagg?cccaggcggt?gaagaacagc?ctgtcccggg?cggccaaggc?ggtgcgacgg????2040

ctacgcgcgg?cgcaccgggt?cgcgctgacc?gggacaccga?tggagaaccg?gctcgccgag????2100

ctgtggtcga?tcatggactt?cctcaacccg?ggcctgctcg?gatcctccga?acgcttccgc????2160

acccgctacg?cgatcccgat?cgagcggcac?gggcacaccg?aaccggccga?acggctgcgc????2220

gcatcgacgc?ggccctacat?cctgcgccgg?ctcaagaccg?acccggcgat?catcgacgat????2280

ctgccggaga?agatcgagat?caagcagtac?tgccaactca?ccaccgagca?ggcgtcgctg????2340

tatcaggccg?tcgtcgccga?catgatggaa?aagatcgaaa?acaccgaagg?gatcgagcgg????2400

cgcggcaacg?tgctggccgc?gatggccaag?ctcaaacagg?tgtgcaacca?ccccgcccag????2460

ctgctgcacg?atcgctcccc?ggtcggtcgg?cggtccggga?aggtgatccg?gctcgaggag????2520

atcctggaag?agatcctggc?cgagggcgac?cgggtgctgt?gttttaccca?gttcaccgag????2580

ttcgccgagc?tgctggtgcc?gcacctggcc?gcacgcttcg?gccgtgccgc?ccgagacatt????2640

gcctacctgc?acggtggcac?cccgaggaag?cggcgtgacg?agatggtggc?ccggttccag????2700

tccggtgacg?gcccgcccat?ttttctgctg?tcgttgaagg?cgggcggtac?cgggctgaac??2760

ctcaccgccg?ccaatcatgt?tgtgcacctg?gaccgctggt?ggaacccggc?ggtcgagaac??2820

caggcgacgg?accgggcgtt?tcggatcggg?cagcggcgca?cggtgcaggt?ccgcaagttc??2880

atctgcaccg?gcaccctcga?ggagaagatc?gacgaaatga?tcgaggagaa?aaaggcgctg??2940

gccgacttgg?tggtcaccga?cggcgaaggc?tggctgaccg?aactgtccac?ccgcgatctg??3000

cgcgaggtgt?tcgcgctgtc?cgaaggcgcc?gtcggtgagt?ag?????????????????????3042

<210>50

<211>1013

<212>PRT

＜213〉Mycobacterium bovis

<400>50

Met?Leu?Val?Leu?His?Gly?Phe?Trp?Ser?Asn?Ser?Gly?Gly?Met?Arg?Leu

1???????????????5???????????????????10??????????????????15

Trp?Ala?Glu?Asp?Ser?Asp?Leu?Leu?Val?Lys?Ser?Pro?Ser?Gln?Ala?Leu

20??????????????????25??????????????????30

Arg?Ser?Ala?Arg?Pro?His?Pro?Phe?Ala?Ala?Pro?Ala?Asp?Leu?Ile?Ala

35??????????????????40??????????????????45

Gly?Ile?His?Pro?Gly?Lys?Pro?Ala?Thr?Ala?Val?Leu?Leu?Leu?Pro?Ser

50??????????????????55??????????????????60

Leu?Arg?Ser?Ala?Pro?Leu?Asp?Ser?Pro?Glu?Leu?Ile?Arg?Leu?Ala?Pro

65??????????????????70??????????????????75??????????????????80

Arg?Pro?Ala?Ala?Arg?Thr?Asp?Pro?Met?Leu?Leu?Ala?Trp?Thr?Val?Pro

85??????????????????90??????????????????95

Val?Val?Asp?Leu?Asp?Pro?Thr?Ala?Ala?Leu?Ala?Ala?Phe?Asp?Gln?Pro

100?????????????????105?????????????????110

Ala?Pro?Asp?Val?Arg?Tyr?Gly?Ala?Ser?Val?Asp?Tyr?Leu?Ala?Glu?Leu

115?????????????????120?????????????????125

Ala?Val?Phe?Ala?Arg?Glu?Leu?Val?Glu?Arg?Gly?Arg?Val?Leu?Pro?Gln

130?????????????????135?????????????????140

Leu?Arg?Arg?Asp?Thr?His?Gly?Ala?Ala?Ala?Cys?Trp?Arg?Pro?Val?Leu

145?????????????????150?????????????????155?????????????????160

Gln?Gly?Arg?Asp?Val?Val?Ala?Met?Thr?Ser?Leu?Val?Ser?Ala?Met?Pro

165?????????????????170?????????????????175

Pro?Val?Cys?Arg?Ala?Glu?Val?Gly?Gly?His?Asp?Pro?His?Glu?Leu?Ala

180?????????????????185?????????????????190

Thr?Ser?Ala?Leu?Asp?Ala?Met?Val?Asp?Ala?Ala?Val?Arg?Ala?Ala?Leu

195?????????????????200?????????????????205

Ser?Pro?Met?Asp?Leu?Leu?Pro?Pro?Arg?Arg?Gly?Arg?Ser?Lys?Arg?His

210?????????????????215?????????????????220

Arg?Ala?Val?Glu?Ala?Trp?Leu?Thr?Ala?Leu?Thr?Cys?Pro?Asp?Gly?Arg

225?????????????????230?????????????????235?????????????????240

Phe?Asp?Ala?Glu?Pro?Asp?Glu?Leu?Asp?Ala?Leu?Ala?Glu?Ala?Leu?Arg

245?????????????????250?????????????????255

Pro?Trp?Asp?Asp?Val?Gly?Ile?Gly?Thr?Val?Gly?Pro?Ala?Arg?Ala?Thr

260?????????????????265?????????????????270

Phe?Arg?Leu?Ser?Glu?Val?Glu?Thr?Glu?Asn?Glu?Glu?Thr?Pro?Ala?Gly

275?????????????????280?????????????????285

Ser?Leu?Trp?Arg?Leu?Glu?Phe?Leu?Leu?Gln?Ser?Thr?Gln?Asp?Pro?Ser

290?????????????????295?????????????????300

Leu?Leu?Val?Pro?Ala?Glu?Gln?Ala?Trp?Asn?Asp?Asp?Gly?Ser?Leu?Arg

305?????????????????310?????????????????315?????????????????320

Arg?Trp?Leu?Asp?Arg?Pro?Gln?Glu?Leu?Leu?Leu?Thr?Glu?Leu?Gly?Arg

325?????????????????330?????????????????335

Ala?Ser?Arg?Ile?Phe?Pro?Glu?Leu?Val?Pro?Ala?Leu?Arg?Thr?Ala?Cys

340?????????????????345?????????????????350

Pro?Ser?Gly?Leu?Glu?Leu?Asp?Ala?Asp?Gly?Ala?Tyr?Arg?Phe?Leu?Ser

355?????????????????360?????????????????365

Gly?Thr?Ala?Ala?Val?Leu?Asp?Glu?Ala?Gly?Phe?Gly?Val?Leu?Leu?Pro

370?????????????????375?????????????????380

Ser?Trp?Trp?Asp?Arg?Arg?Arg?Lys?Leu?Gly?Leu?Val?Leu?Ser?Ala?Tyr

385?????????????????390?????????????????395?????????????????400

Thr?Pro?Val?Asp?Gly?Val?Val?Gly?Lys?Ala?Ser?Lys?Phe?Gly?Arg?Glu

405?????????????????410?????????????????415

Gln?Leu?Val?Glu?Phe?Arg?Trp?Glu?Leu?Ala?Val?Gly?Asp?Asp?Pro?Leu

420?????????????????425?????????????????430

Ser?Glu?Glu?Glu?Ile?Ala?Ala?Leu?Thr?Glu?Thr?Lys?Ser?Pro?Leu?Ile

435?????????????????440?????????????????445

Arg?Leu?Arg?Gly?Gln?Trp?Val?Ala?Leu?Asp?Thr?Glu?Gln?Leu?Arg?Arg

450?????????????????455?????????????????460

Gly?Leu?Glu?Phe?Leu?Glu?Arg?Lys?Pro?Thr?Gly?Arg?Lys?Thr?Thr?Ala

465?????????????????470?????????????????475?????????????????480

Glu?Ile?Leu?Ala?Leu?Ala?Ala?Ser?His?Pro?Asp?Asp?Val?Asp?Thr?Pro

485?????????????????490?????????????????495

Leu?Glu?Val?Thr?Ala?Val?Arg?Ala?Asp?Gly?Trp?Leu?Gly?Asp?Leu?Leu

500?????????????????505?????????????????510

Ala?Gly?Ala?Ala?Ala?Ala?Ser?Leu?Gln?Pro?Leu?Asp?Pro?Pro?Asp?Gly

515?????????????????520?????????????????525

Phe?Thr?Ala?Thr?Leu?Arg?Pro?Tyr?Gln?Gln?Arg?Gly?Leu?Ala?Trp?Leu

530?????????????????535?????????????????540

Ala?Phe?Leu?Ser?Ser?Leu?Gly?Leu?Gly?Ser?Cys?Leu?Ala?Asp?Asp?Met

545?????????????????550?????????????????555?????????????????560

Gly?Leu?Gly?Lys?Thr?Val?Gln?Leu?Leu?Ala?Leu?Glu?Thr?Leu?Glu?Ser

565?????????????????570?????????????????575

Val?Gln?Arg?His?Gln?Asp?Arg?Gly?Val?Gly?Pro?Thr?Leu?Leu?Leu?Cys

580?????????????????585?????????????????590

Pro?Met?Ser?Leu?Val?Gly?Asn?Trp?Gln?Gln?Glu?Ala?Ala?Arg?Phe?Ala

595?????????????????600?????????????????605

Pro?Asn?Leu?Arg?Val?Tyr?Ala?His?His?Gly?Gly?Ala?Arg?Leu?His?Gly

610?????????????????615?????????????????620

Glu?Ala?Leu?Arg?Asp?His?Leu?Glu?Arg?Thr?Asp?Leu?Val?Val?Ser?Thr

625?????????????????630?????????????????635?????????????????640

Tyr?Thr?Thr?Ala?Thr?Arg?Asp?Ile?Asp?Glu?Leu?Ser?Glu?Tyr?Glu?Trp

645?????????????????650?????????????????655

Asn?Arg?Val?Val?Leu?Asp?Glu?Ala?Gln?Ala?Val?Lys?Asn?Ser?Leu?Ser

660?????????????????665?????????????????670

Arg?Ala?Ala?Lys?Ala?Val?Arg?Arg?Leu?Arg?Ala?Ala?His?Arg?Val?Ala

675?????????????????680?????????????????685

Leu?Thr?Gly?Thr?Pro?Met?Glu?Asn?Arg?Leu?Ala?Glu?Leu?Trp?Ser?Ile

690?????????????????695?????????????????700

Met?Asp?Phe?Leu?Asn?Pro?Gly?Leu?Leu?Gly?Ser?Ser?Glu?Arg?Phe?Arg

705?????????????????710?????????????????715?????????????????720

Thr?Arg?Tyr?Ala?Ile?Pro?Ile?Glu?Arg?His?Gly?His?Thr?Glu?Pro?Ala

725?????????????????730?????????????????735

Glu?Arg?Leu?Arg?Ala?Ser?Thr?Arg?Pro?Tyr?Ile?Leu?Arg?Arg?Leu?Lys

740?????????????????745?????????????????750

Thr?Asp?Pro?Ala?Ile?Ile?Asp?Asp?Leu?Pro?Glu?Lys?Ile?Glu?Ile?Lys

755?????????????????760?????????????????765

Gln?Tyr?Cys?Gln?Leu?Thr?Thr?Glu?Gln?Ala?Ser?Leu?Tyr?Gln?Ala?Val

770?????????????????775?????????????????780

Val?Ala?Asp?Met?Met?Glu?Lys?Ile?Glu?Asn?Thr?Glu?Gly?Ile?Glu?Arg

785?????????????????790?????????????????795?????????????????800

Arg?Gly?Asn?Val?Leu?Ala?Ala?Met?Ala?Lys?Leu?Lys?Gln?Val?Cys?Asn

805?????????????????810?????????????????815

His?Pro?Ala?Gln?Leu?Leu?His?Asp?Arg?Ser?Pro?Val?Gly?Arg?Arg?Ser

820?????????????????825?????????????????830

Gly?Lys?Val?Ile?Arg?Leu?Glu?Glu?Ile?Leu?Glu?Glu?Ile?Leu?Ala?Glu

835?????????????????840?????????????????845

Gly?Asp?Arg?Val?Leu?Cys?Phe?Thr?Gln?Phe?Thr?Glu?Phe?Ala?Glu?Leu

850?????????????????855?????????????????860

Leu?Val?Pro?His?Leu?Ala?Ala?Arg?Phe?Gly?Arg?Ala?Ala?Arg?Asp?Ile

865?????????????????870?????????????????875?????????????????880

Ala?Tyr?Leu?His?Gly?Gly?Thr?Pro?Arg?Lys?Arg?Arg?Asp?Glu?Met?Val

885?????????????????890?????????????????895

Ala?Arg?Phe?Gln?Ser?Gly?Asp?Gly?Pro?Pro?Ile?Phe?Leu?Leu?Ser?Leu

900?????????????????905?????????????????910

Lys?Ala?Gly?Gly?Thr?Gly?Leu?Asn?Leu?Thr?Ala?Ala?Asn?His?Val?Val

915?????????????????920?????????????????925

His?Leu?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp

930?????????????????935?????????????????940

Arg?Ala?Phe?Arg?Ile?Gly?Gln?Arg?Arg?Thr?Val?Gln?Val?Arg?Lys?Phe

945?????????????????950?????????????????955?????????????????960

Ile?Cys?Thr?Gly?Thr?Leu?Glu?Glu?Lys?Ile?Asp?Glu?Met?Ile?Glu?Glu

965?????????????????970?????????????????975

Lys?Lys?Ala?Leu?Ala?Asp?Leu?Val?Val?Thr?Asp?Gly?Glu?Gly?Trp?Leu

980?????????????????985?????????????????990

Thr?Glu?Leu?Ser?Thr?Arg?Asp?Leu??Arg?Glu?Val?Phe?Ala??Leu?Ser?Glu

995?????????????????1000?????????????????1005

Gly?Ala??Val?Gly?Glu

1010

<210>51

<211>3042

<212>DNA

＜213〉mycobacterium tuberculosis

<400>51

atgctggttt?tgcacggctt?ctggtccaac?tccggcggga?tgcggctgtg?ggcggaggac???60

tccgatctgc?tggtgaagag?cccgagtcag?gcgctgcgct?ccgcgcggcc?acacccgttc??120

gcggcgcccg?ctgacctgat?cgccggcata?catccgggca?aacccgcaac?cgccgttttg??180

ctgttgccgt?cgttgcgatc?ggcgccgctg?gactcgccgg?agctgatccg?gctcgccccg??240

cgcccggccg?cgcgaaccga?tccgatgctg?ttggcgtgga?cggtaccggt?ggtggacctg??300

gaccccaccg?cggcgttggc?cgccttcgac?cagcccgccc?ccgacgtccg?ctacggcgcg??360

tccgtcgact?acctggccga?gctggccgtt?ttcgcgcgcg?agttggtcga?gcgtggtcgc??420

gtgctgcccc?agctgcgccg?cgacacccac?ggcgcggccg?cctgctggcg?tccggtgttg??480

cagggacgcg?acgtggtcgc?gatgacctcg?ctggtctcgg?cgatgccgcc?ggtctgccgc??540

gccgaagttg?gtgggcacga?cccgcacgaa?ctggcaacct?cggctctgga?cgcgatggtc??600

gacgccgccg?tgcgcgcggc?gctgtcaccg?atggacctgc?tgcccccgcg?acggggtcgc??660

tccaaacggc?atcgggccgt?ggaggcttgg?ctgaccgcgt?tgacctgccc?ggacggccgg??720

ttcgacgcgg?agcccgacga?actcgacgcg?ctggccgagg?cgttgcggcc?atgggacgac??780

gtcggtatcg?gcaccgtcgg?cccggcgcgg?gcgacgtttc?ggctgtccga?agtcgagacc?????840

gaaaacgagg?agacgcccgc?gggctcgttg?tggaggctgg?agttcttatt?gcagtcgacg?????900

caggacccca?gcctgctggt?ccccgccgag?caggcatgga?acgacgacgg?cagcctgcgc?????960

cgctggctgg?accggccgca?ggagctgctg?ctgaccgaac?tgggccgggc?ctctcggatt????1020

ttccccgagc?tcgtcccggc?gctgcgcacc?gcgtgcccgt?ccgggcttga?gctcgacgcc????1080

gacggcgcct?accgattcct?gtcgggtacg?gccgcggtgc?tcgacgaggc?tgggtttggc????1140

gtgctgctgc?cgtcctggtg?ggaccgccgc?cgcaagctgg?gcttggtcct?gtccgcatat????1200

accccggtcg?acggcgtggt?gggcaaggcc?agcaagttcg?gccgcgagca?gctcgtcgag????1260

ttccgctggg?agctggccgt?gggcgacgat?ccgctcagcg?aggaggagat?cgcggcgctg????1320

accgaaacca?agtccccgct?gatccggctg?cgtggccagt?gggtcgcgct?cgataccgaa????1380

cagatgcgcc?gcgggctgga?gtttttggag?cgtaagccaa?ccggccgcaa?gaccaccgcc????1440

gagatcctcg?cgctggccgc?cagccacccc?gacgacgtgg?acaccccgct?cgaggtcacc????1500

gccgtacgcg?ccgacggctg?gctcggggac?ctgctcgccg?gggccgccgc?ggcgtcgctg????1560

cagccgttgg?acccgcccga?cggattcacc?gcgacgctgc?gtccctacca?gcagcgcggt????1620

ctggcgtggc?tggcgttttt?gtcctcgctc?ggtttgggca?gctgcctggc?cgacgacatg????1680

ggcctgggca?agacggtgca?gctattggcc?ctggaaacct?tggaatccgt?tcagcgccac????1740

caggatcgcg?gcgtcggacc?cacactgcta?ctgtgcccga?tgtcgttggt?gggcaactgg????1800

ccgcaggaag?cggccaggtt?tgcacccaac?ctgcgggtgt?acgcccacca?cgggggcgcc????1860

cggctgcacg?gcgaggcgtt?gcgcgaccac?ctcgagcgca?ccgacctggt?cgtgagcacc????1920

tataccaccg?ccacccgcga?catcgacgag?ctggcggaat?acgaatggaa?ccgggtggtg????1980

ctggacgagg?cccaggcggt?gaagaacagc?ctgtcccggg?cggccaaggc?ggtgcgacgg????2040

ctacgcgcgg?cgcaccgggt?cgcgctgacc?gggacaccga?tggagaaccg?gctcgccgag????2100

ctgtggtcga?tcatggactt?cctcaacccg?ggcctgctcg?gatcctccga?acgcttccgc??2160

acccgctacg?cgatcccgat?cgagcggcac?gggcacaccg?aaccggccga?acggctgcgc??2220

gcatcgacgc?ggccctacat?cctgcgccgg?ctcaagaccg?acccggcgat?catcgacgat??2280

ctgccggaga?agatcgagat?caagcagtac?tgccaactca?ccaccgagca?ggcgtcgctg??2340

tatcaggccg?tcgtcgccga?catgatggaa?aagatcgaaa?acaccgaagg?gatcgagcgg??2400

cgcggcaacg?tgctggccgc?gatggccaag?ctcaaacagg?tgtgcaacca?ccccgcccag??2460

ctgctgcacg?atcgctcccc?ggtcggtcgg?cggtccggga?aggtgatccg?gctcgaggag??2520

atcctggaag?agatcctggc?cgagggcgac?cgggtgctgt?gttttaccca?gttcaccgag??2580

ttcgccgagc?tgctggtgcc?gcacctggcc?gcacgcttcg?gccgtgccgc?ccgagacatt??2640

gcctacctgc?acggtggcac?cccgaggaag?cggcgtgacg?agatggtggc?ccggttccag??2700

tccggtgacg?gcccgcccat?ttttctgctg?tcgttgaagg?cgggcggtac?cgggctgaac??2760

ctcaccgccg?ccaatcatgt?tgtgcacctg?gaccgctggt?ggaacccggc?ggtcgagaac??2820

caggcgacgg?accgggcgtt?tcggatcggg?cagcggcgca?cggtgcaggt?ccgcaagttc??2880

atctgcaccg?gcaccctcga?ggagaagatc?gacgaaatga?tcgaggagaa?aaaggcgctg??2940

gccgacttgg?tggtcaccga?cggcgaaggc?tggctgaccg?aactgtccac?ccgcgatctg??3000

cgcgaggtgt?tcgcgctgtc?cgaaggcgcc?gtcggtgagt?ag?????????????????????3042

<210>52

<211>1013

<212>PRT

＜213〉mycobacterium tuberculosis

<400>52

Met?Leu?Val?Leu?His?Gly?Phe?Trp?Ser?Asn?Ser?Gly?Gly?Met?Arg?Leu

1???????????????5???????????????????10??????????????????15

Trp?Ala?Glu?Asp?Ser?Asp?Leu?Leu?Val?Lys?Ser?Pro?Ser?Gln?Ala?Leu

20??????????????????25??????????????????30

Arg?Ser?Ala?Arg?Pro?His?Pro?Phe?Ala?Ala?Pro?Ala?Asp?Leu?Ile?Ala

35??????????????????40??????????????????45

Gly?Ile?His?Pro?Gly?Lys?Pro?Ala?Thr?Ala?Val?Leu?Leu?Leu?Pro?Ser

50??????????????????55??????????????????60

Leu?Arg?Ser?Ala?Pro?Leu?Asp?Ser?Pro?Glu?Leu?Ile?Arg?Leu?Ala?Pro

65??????????????????70??????????????????75??????????????????80

Arg?Pro?Ala?Ala?Arg?Thr?Asp?Pro?Met?Leu?Leu?Ala?Trp?Thr?Val?Pro

85??????????????????90??????????????????95

Val?Val?Asp?Leu?Asp?Pro?Thr?Ala?Ala?Leu?Ala?Ala?Phe?Asp?Gln?Pro

100?????????????????105?????????????????110

Ala?Pro?Asp?Val?Arg?Tyr?Gly?Ala?Ser?Val?Asp?Tyr?Leu?Ala?Glu?Leu

115?????????????????120?????????????????125

Ala?Val?Phe?Ala?Arg?Glu?Leu?Val?Glu?Arg?Gly?Arg?Val?Leu?Pro?Gln

130?????????????????135?????????????????140

Leu?Arg?Arg?Asp?Thr?His?Gly?Ala?Ala?Ala?Cys?Trp?Arg?Pro?Val?Leu

145?????????????????150?????????????????155?????????????????160

Gln?Gly?Arg?Asp?Val?Val?Ala?Met?Thr?Ser?Leu?Val?Ser?Ala?Met?Pro

165?????????????????170?????????????????175

Pro?Val?Cys?Arg?Ala?Glu?Val?Gly?Gly?His?Asp?Pro?His?Glu?Leu?Ala

180?????????????????185?????????????????190

Thr?Ser?Ala?Leu?Asp?Ala?Met?Val?Asp?Ala?Ala?Val?Arg?Ala?Ala?Leu

195?????????????????200?????????????????205

Ser?Pro?Met?Asp?Leu?Leu?Pro?Pro?Arg?Arg?Gly?Arg?Ser?Lys?Arg?His

210?????????????????215?????????????????220

Arg?Ala?Val?Glu?Ala?Trp?Leu?Thr?Ala?Leu?Thr?Cys?Pro?Asp?Gly?Arg

225?????????????????230?????????????????235?????????????????240

Phe?Asp?Ala?Glu?Pro?Asp?Glu?Leu?Asp?Ala?Leu?Ala?Glu?Ala?Leu?Arg

245?????????????????250?????????????????255

Pro?Trp?Asp?Asp?Val?Gly?Ile?Gly?Thr?Val?Gly?Pro?Ala?Arg?Ala?Thr

260?????????????????265?????????????????270

Phe?Arg?Leu?Ser?Glu?Val?Glu?Thr?Glu?Asn?Glu?Glu?Thr?Pro?Ala?Gly

275?????????????????280?????????????????285

Ser?Leu?Trp?Arg?Leu?Glu?Phe?Leu?Leu?Gln?Ser?Thr?Gln?Asp?Pro?Ser

290?????????????????295?????????????????300

Leu?Leu?Val?Pro?Ala?Glu?Gln?Ala?Trp?Asn?Asp?Asp?Gly?Ser?Leu?Arg

305?????????????????310?????????????????315?????????????????320

Arg?Trp?Leu?Asp?Arg?Pro?Gln?Glu?Leu?Leu?Leu?Thr?Glu?Leu?Gly?Arg

325?????????????????330?????????????????335

Ala?Ser?Arg?Ile?Phe?Pro?Glu?Leu?Val?Pro?Ala?Leu?Arg?Thr?Ala?Cys

340?????????????????345?????????????????350

Pro?Ser?Gly?Leu?Glu?Leu?Asp?Ala?Asp?Gly?Ala?Tyr?Arg?Phe?Leu?Ser

355?????????????????360?????????????????365

Gly?Thr?Ala?Ala?Val?Leu?Asp?Glu?Ala?Gly?Phe?Gly?Val?Leu?Leu?Pro

370?????????????????375?????????????????380

Ser?Trp?Trp?Asp?Arg?Arg?Arg?Lys?Leu?Gly?Leu?Val?Leu?Ser?Ala?Tyr

385?????????????????390?????????????????395?????????????????400

Thr?Pro?Val?Asp?Gly?Val?Val?Gly?Lys?Ala?Ser?Lys?Phe?Gly?Arg?Glu

405?????????????????410?????????????????415

Gln?Leu?Val?Glu?Phe?Arg?Trp?Glu?Leu?Ala?Val?Gly?Asp?Asp?Pro?Leu

420?????????????????425?????????????????430

Ser?Glu?Glu?Glu?Ile?Ala?Ala?Leu?Thr?Glu?Thr?Lys?Ser?Pro?Leu?Ile

435?????????????????440?????????????????445

Arg?Leu?Arg?Gly?Gln?Trp?Val?Ala?Leu?Asp?Thr?Glu?Gln?Met?Arg?Arg

450?????????????????455?????????????????460

Gly?Leu?Glu?Phe?Leu?Glu?Arg?Lys?Pro?Thr?Gly?Arg?Lys?Thr?Thr?Ala

465?????????????????470?????????????????475?????????????????480

Glu?Ile?Leu?Ala?Leu?Ala?Ala?Ser?His?Pro?Asp?Asp?Val?Asp?Thr?Pro

485?????????????????490?????????????????495

Leu?Glu?Val?Thr?Ala?Val?Arg?Ala?Asp?Gly?Trp?Leu?Gly?Asp?Leu?Leu

500?????????????????505?????????????????510

Ala?Gly?Ala?Ala?Ala?Ala?Ser?Leu?Gln?Pro?Leu?Asp?Pro?Pro?Asp?Gly

515?????????????????520?????????????????525

Phe?Thr?Ala?Thr?Leu?Arg?Pro?Tyr?Gln?Gln?Arg?Gly?Leu?Ala?Trp?Leu

530?????????????????535?????????????????540

Ala?Phe?Leu?Ser?Ser?Leu?Gly?Leu?Gly?Ser?Cys?Leu?Ala?Asp?Asp?Met

545?????????????????550?????????????????555?????????????????560

Gly?Leu?Gly?Lys?Thr?Val?Gln?Leu?Leu?Ala?Leu?Glu?Thr?Leu?Glu?Ser

565?????????????????570?????????????????575

Val?Gln?Arg?His?Gln?Asp?Arg?Gly?Val?Gly?Pro?Thr?Leu?Leu?Leu?Cys

580?????????????????585?????????????????590

Pro?Met?Ser?Leu?Val?Gly?Asn?Trp?Pro?Gln?Glu?Ala?Ala?Arg?Phe?Ala

595?????????????????600?????????????????605

Pro?Asn?Leu?Arg?Val?Tyr?Ala?His?His?Gly?Gly?Ala?Arg?Leu?His?Gly

610?????????????????615?????????????????620

Glu?Ala?Leu?Arg?Asp?His?Leu?Glu?Arg?Thr?Asp?Leu?Val?Val?Ser?Thr

625?????????????????630?????????????????635?????????????????640

Tyr?Thr?Thr?Ala?Thr?Arg?Asp?Ile?Asp?Glu?Leu?Ala?Glu?Tyr?Glu?Trp

645?????????????????650?????????????????655

Asn?Arg?Val?Val?Leu?Asp?Glu?Ala?Gln?Ala?Val?Lys?Asn?Ser?Leu?Ser

660?????????????????665?????????????????670

Arg?Ala?Ala?Lys?Ala?Val?Arg?Arg?Leu?Arg?Ala?Ala?His?Arg?Val?Ala

675?????????????????680?????????????????685

Leu?Thr?Gly?Thr?Pro?Met?Glu?Asn?Arg?Leu?Ala?Glu?Leu?Trp?Ser?Ile

690?????????????????695?????????????????700

Met?Asp?Phe?Leu?Asn?Pro?Gly?Leu?Leu?Gly?Ser?Ser?Glu?Arg?Phe?Arg

705?????????????????710?????????????????715?????????????????720

Thr?Arg?Tyr?Ala?Ile?Pro?Ile?Glu?Arg?His?Gly?His?Thr?Glu?Pro?Ala

725?????????????????730?????????????????735

Glu?Arg?Leu?Arg?Ala?Ser?Thr?Arg?Pro?Tyr?Ile?Leu?Arg?Arg?Leu?Lys

740?????????????????745?????????????????750

Thr?Asp?Pro?Ala?Ile?Ile?Asp?Asp?Leu?Pro?Glu?Lys?Ile?Glu?Ile?Lys

755?????????????????760?????????????????765

Gln?Tyr?Cys?Gln?Leu?Thr?Thr?Glu?Gln?Ala?Ser?Leu?Tyr?Gln?Ala?Val

770?????????????????775?????????????????780

Val?Ala?Asp?Met?Met?Glu?Lys?Ile?Glu?Asn?Thr?Glu?Gly?Ile?Glu?Arg

785?????????????????790?????????????????795?????????????????800

Arg?Gly?Asn?Val?Leu?Ala?Ala?Met?Ala?Lys?Leu?Lys?Gln?Val?Cys?Asn

805?????????????????810?????????????????815

His?Pro?Ala?Gln?Leu?Leu?His?Asp?Arg?Ser?Pro?Val?Gly?Arg?Arg?Ser

820?????????????????825?????????????????830

Gly?Lys?Val?Ile?Arg?Leu?Glu?Glu?Ile?Leu?Glu?Glu?Ile?Leu?Ala?Glu

835?????????????????840?????????????????845

Gly?Asp?Arg?Val?Leu?Cys?Phe?Thr?Gln?Phe?Thr?Glu?Phe?Ala?Glu?Leu

850?????????????????855?????????????????860

Leu?Val?Pro?His?Leu?Ala?Ala?Arg?Phe?Gly?Arg?Ala?Ala?Arg?Asp?Ile

865?????????????????870?????????????????875?????????????????880

Ala?Tyr?Leu?His?Gly?Gly?Thr?Pro?Arg?Lys?Arg?Arg?Asp?Glu?Met?Val

885?????????????????890?????????????????895

Ala?Arg?Phe?Gln?Ser?Gly?Asp?Gly?Pro?Pro?Ile?Phe?Leu?Leu?Ser?Leu

900?????????????????905?????????????????910

Lys?Ala?Gly?Gly?Thr?Gly?Leu?Asn?Leu?Thr?Ala?Ala?Asn?His?Val?Val

915?????????????????920?????????????????925

His?Leu?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp

930?????????????????935?????????????????940

Arg?Ala?Phe?Arg?Ile?Gly?Gln?Arg?Arg?Thr?Val?Gln?Val?Arg?Lys?Phe

945?????????????????950?????????????????955?????????????????960

Ile?Cys?Thr?Gly?Thr?Leu?Glu?Glu?Lys?Ile?Asp?Glu?Met?Ile?Glu?Glu

965?????????????????970?????????????????975

Lys?Lys?Ala?Leu?Ala?Asp?Leu?Val?Val?Thr?Asp?Gly?Glu?Gly?Trp?Leu

980?????????????????985?????????????????990

Thr?Glu?Leu?Ser?Thr?Arg?Asp?Leu??Arg?Glu?Val?Phe?Ala??Leu?Ser?Glu

995?????????????????1000?????????????????1005

Gly?Ala??Val?Gly?Glu

1010

<210>53

<211>3282

<212>DNA

＜213〉yellow myxococcus

<400>53

gtgcgagcct?ggaggggcgt?cctccgctgg?gctgccgctg?gcctctccct?gtccgcggct?????60

cggagtccga?ccggccacct?cccagtgttt?tcaggttttt?ccgtggcgac?cgatggcgtc????120

gggctgttcg?cgggtctgtc?tgttcgggcc?cttgtccatc?aagggcctgg?aggaggaccg????180

ctacgagcgc?ctcacggaca?acccggcagg?cctgcggctc?acggagccgg?caatcccgtg????240

caggggcgct?cgcaggcctg?cttgcgtgtg?ccgcttgccc?ggacggagtt?tacattcgca????300

gcgatgcccc?tcgtgttcct?gcccgacgcc?gagacgctgt?tcctctgggg?gcccgaccgg????360

ctgccacgtg?agctcgccgg?cctgccggag?acgggggacc?gcgcctccgc?gctgctcgtg????420

acgcccgagg?gattgcgtga?atgcgagggg?cacgggctgc?ccctggccgc?caccgtcgag????480

cggctcgcgg?tggtgcaaac?ctccgaggcc?gagtcctttc?ctggctccat?cgccctgtgg????540

acgctggcca?gcaagctcgc?gctggagttg?gtggcgcgcg?agcgcgtggt?gcccacgctc????600

ctgcggcggg?gcgagcgcat?cgaggctcgc?tgggcggcgg?ccctctccgc?caccgaggac????660

gccggccgcg?tcgccgcgct?cgcccggagc?atgccgcccg?gcgcgcacgc?cgtccccgca????720

ggcgccaggc?caggccgcgc?cgtctgggcc?ccggacgcct?tgctgcgcgc?cttcctcgac????780

gccaccgtcg?acgccttcgt?gcgcgccgcg?cgcggtgcgc?cttcgttgcc?ggcccggcgc????840

gcggcctcgt?gggacgagcg?ctggcgcgag?gcgctcaccg?gcgcgcgacg?cgacttcgcg????900

ccggagggct?tcgccgagcg?ctccgtcgtc?gatgagctga?cgcgctggag?cgaacccgcg????960

ctcggcgccc?gggacaagct?gcgcgcctgc?ttccggctgg?agcccccgac?ggaggagcgc???1020

gagcccttcg?tgctgagctt?ccacctccag?tccccggacg?acccaagcct?gctcgtcccg???1080

gccgcggacg?tctggaagac?gcgcgggcgc?agcctggaga?agctcggccg?cgccttccgt???1140

gacccgcagg?agtccctgct?cgaggcactc?ggccgcgccg?cccggctctt?ccccccgctg???1200

gcgctcgtgc?tggagagccc?acgtccccag?gcgctcctgc?tcgagcccga?caccgcgtgg???1260

acgttcctct?cggagggcgc?ccgcgtgctc?tcagacgccg?gcttcggcgt?catcgtccct???1320

ggcgagctca?ccacctcggg?ccgacgccgc?ctgcgcctgc?gcatgcgcgt?gggcgcgagc???1380

acgaaggccg?cgggggccgt?cggtggcacc?gcggggctcg?ggctcgacgc?gctgctgcgc???1440

gtggactggg?acgccgtgct?gggcgaccaa?cccctctccg?cccaggagct?ggcgctgctg???1500

gcccagcgca?aggccccgct?cgtgcgattc?cgcggcgagt?gggtcgcggt?ggatcccctc????1560

gaactcgacg?ccatccagcg?ccacctcgcc?cagggccccg?gccgcatggc?gctgagcgag????1620

gcggtgcggg?tgtccctgct?aggcgaaacg?cgccacggac?agctccccgt?caccgttctc????1680

gccaccgggg?cgctggagga?gcgcctgcgc?ctgcttcggg?agggcggggc?caccgctcag????1740

gacgcccccc?gcgcgctgcg?cgccacgctg?cggccctacc?agtcgcgcgg?tctgcactgg????1800

ctggacacgc?tggcctcatt?ggggctcggc?gcctgcctcg?cggacgacat?gggcctgggc????1860

aagacggtgc?aggtgctggc?cttcctgctg?cggcggctcg?agcaggcgcc?tgacgaggcg????1920

cgccccacgc?tgctggtggc?ccccacctcc?gtggtgggca?actgggagcg?tgagctcgcc????1980

cgcttcgccc?ccaccttgcg?cctgacgcgg?cactacggcg?ccgagcgcgc?ccgcgcggcg????2040

aaccgcttcc?cccgcgcgcc?cggcgccgtc?gtgctcacca?cctacggctt?gctgcgccgg????2100

gacgccgcgc?tgctcgcgcg?cgtggactgg?ggcgcggtgg?tgctcgacga?ggcgcagaac????2160

atcaagaacg?cggcgtcggc?taccgcccgc?gcggcccggg?cgttgcgcgc?cagccagcgc????2220

ttcgcgctca?cgggcacgcc?ggtggagaac?cgcctggcgg?agctgtggtc?catcctcgag????2280

ttcgccaacc?cgggcctgct?cgggccgctg?gagacgttcc?ggcgggagct?ggcgctgccc????2340

attgaacgcc?atggcaatca?ggaggcctcg?gcccggctgc?gccggctcgt?gagccccttc????2400

gtcctgcgcc?gcctcaagag?cgacccgacc?atcatcacgg?acctgcccgc?gaagaatgag????2460

atgaaggtcg?tctgcacgct?cacgcgcgag?caggcctcgc?tctacaaggc?ggtggtggac????2520

gaggagctgc?ggcgcatcga?ggaggccgac?ggcatggagc?gccggggccg?cgtgctcgcg????2580

ctgctgctgt?acacgaagca?gatcgccaac?cacccggcgc?agtacctcgg?ggagtccggg????2640

cccctgccgg?ggcgctcggg?gaagctggcg?cgcgtggtgg?agatgctcga?ggagtccctg????2700

gccgctggcg?acaaggcgct?cgtcttcacg?cagttccggg?agatgggcga?caagctggtg????2760

gcgcacctgt?cggagtacct?gggccacgag?gtgctcttcc?tccacggcgg?cacgccccgc????2820

aaggcgcgcg?acgagatggt?gcggcgcttc?caggaggacg?tccacggtcc?gcgtgtgttc??2880

gtgctgtccg?tcaaggcggg?aggcacgggg?ctcaacctga?cggcggcgag?ccatgtgttc??2940

cattacgacc?gctggtggaa?cccggccgtc?gaggaccagg?ccaccgaccg?cgcgtaccgc??3000

atcgggcaga?cgcgcgcggt?gcaggtccac?aagctggtgt?gtgcgggcac?tgtcgaggag??3060

aaggtggacc?ggctgctcga?acagaagcgc?cagctcgccg?agaaggtcgt?gggcgcgggc??3120

gagcactggg?tgaccgagct?ggacacgacg?gcgctgcgcg?agctgttctc?gctgtccgag??3180

ggcgccgtgg?cggacgatgg?cgacgcggaa?ggggaagacg?acgcgcgggt?gcgcgccccg??3240

cgacggcgcg?gccgtgcgag?cgcgaaggcg?gtgtcgcgat?ga?????????????????????3282

<210>54

<211>1093

<212>PRT

＜213〉yellow myxococcus

<400>54

Val?Arg?Ala?Trp?Arg?Gly?Val?Leu?Arg?Trp?Ala?Ala?Ala?Gly?Leu?Ser

1???????????????5???????????????????10??????????????????15

Leu?Ser?Ala?Ala?Arg?Ser?Pro?Thr?Gly?His?Leu?Pro?Val?Phe?Ser?Gly

20??????????????????25??????????????????30

Phe?Ser?Val?Ala?Thr?Asp?Gly?Val?Gly?Leu?Phe?Ala?Gly?Leu?Ser?Val

35??????????????????40??????????????????45

Arg?Ala?Leu?Val?His?Gln?Gly?Pro?Gly?Gly?Gly?Pro?Leu?Arg?Ala?Pro

50??????????????????55??????????????????60

His?Gly?Gln?Pro?Gly?Arg?Pro?Ala?Ala?His?Gly?Ala?Gly?Asn?Pro?Val

65??????????????????70??????????????????75??????????????????80

Gln?Gly?Arg?Ser?Gln?Ala?Cys?Leu?Arg?Val?Pro?Leu?Ala?Arg?Thr?Glu

85??????????????????90??????????????????95

Phe?Thr?Phe?Ala?Ala?Met?Pro?Leu?Val?Phe?Leu?Pro?Asp?Ala?Glu?Thr

100?????????????????105?????????????????110

Leu?Phe?Leu?Trp?Gly?Pro?Asp?Arg?Leu?Pro?Arg?Glu?Leu?Ala?Gly?Leu

115?????????????????120?????????????????125

Pro?Glu?Thr?Gly?Asp?Arg?Ala?Ser?Ala?Leu?Leu?Val?Thr?Pro?Glu?Gly

130?????????????????135?????????????????140

Leu?Arg?Glu?Cys?Glu?Gly?His?Gly?Leu?Pro?Leu?Ala?Ala?Thr?Val?Glu

145?????????????????150?????????????????155?????????????????160

Arg?Leu?Ala?Val?Val?Gln?Thr?Ser?Glu?Ala?Glu?Ser?Phe?Pro?Gly?Ser

165?????????????????170?????????????????175

Ile?Ala?Leu?Trp?Thr?Leu?Ala?Ser?Lys?Leu?Ala?Leu?Glu?Leu?Val?Ala

180?????????????????185?????????????????190

Arg?Glu?Arg?Val?Val?Pro?Thr?Leu?Leu?Arg?Arg?Gly?Glu?Arg?Ile?Glu

195?????????????????200?????????????????205

Ala?Arg?Trp?Ala?Ala?Ala?Leu?Ser?Ala?Thr?Glu?Asp?Ala?Gly?Arg?Val

210?????????????????215?????????????????220

Ala?Ala?Leu?Ala?Arg?Ser?Met?Pro?Pro?Gly?Ala?His?Ala?Val?Pro?Ala

225?????????????????230?????????????????235?????????????????240

Gly?Ala?Arg?Pro?Gly?Arg?Ala?Val?Trp?Ala?Pro?Asp?Ala?Leu?Leu?Arg

245?????????????????250?????????????????255

Ala?Phe?Leu?Asp?Ala?Thr?Val?Asp?Ala?Phe?Val?Arg?Ala?Ala?Arg?Gly

260?????????????????265?????????????????270

Ala?Pro?Ser?Leu?Pro?Ala?Arg?Arg?Ala?Ala?Ser?Trp?Asp?Glu?Arg?Trp

275?????????????????280?????????????????285

Arg?Glu?Ala?Leu?Thr?Gly?Ala?Arg?Arg?Asp?Phe?Ala?Pro?Glu?Gly?Phe

290?????????????????295?????????????????300

Ala?Glu?Arg?Ser?Val?Val?Asp?Glu?Leu?Thr?Arg?Trp?Ser?Glu?Pro?Ala

305?????????????????310?????????????????315?????????????????320

Leu?Gly?Ala?Arg?Asp?Lys?Leu?Arg?Ala?Cys?Phe?Arg?Leu?Glu?Pro?Pro

325?????????????????330?????????????????335

Thr?Glu?Glu?Arg?Glu?Pro?Phe?Val?Leu?Ser?Phe?His?Leu?Gln?Ser?Pro

340?????????????????345?????????????????350

Asp?Asp?Pro?Ser?Leu?Leu?Val?Pro?Ala?Ala?Asp?Val?Trp?Lys?Thr?Arg

355?????????????????360?????????????????365

Gly?Arg?Ser?Leu?Glu?Lys?Leu?Gly?Arg?Ala?Phe?Arg?Asp?Pro?Gln?Glu

370?????????????????375?????????????????380

Ser?Leu?Leu?Glu?Ala?Leu?Gly?Arg?Ala?Ala?Arg?Leu?Phe?Pro?Pro?Leu

385?????????????????390?????????????????395?????????????????400

Ala?Leu?Val?Leu?Glu?Ser?Pro?Arg?Pro?Gln?Ala?Leu?Leu?Leu?Glu?Pro

405?????????????????410?????????????????415

Asp?Thr?Ala?Trp?Thr?Phe?Leu?Ser?Glu?Gly?Ala?Arg?Val?Leu?Ser?Asp

420?????????????????425?????????????????430

Ala?Gly?Phe?Gly?Val?Ile?Val?Pro?Gly?Glu?Leu?Thr?Thr?Ser?Gly?Arg

435?????????????????440?????????????????445

Arg?Arg?Leu?Arg?Leu?Arg?Met?Arg?Val?Gly?Ala?Ser?Thr?Lys?Ala?Ala

450?????????????????455?????????????????460

Gly?Ala?Val?Gly?Gly?Thr?Ala?Gly?Leu?Gly?Leu?Asp?Ala?Leu?Leu?Arg

465?????????????????470?????????????????475?????????????????480

Val?Asp?Trp?Asp?Ala?Val?Leu?Gly?Asp?Gln?Pro?Leu?Ser?Ala?Gln?Glu

485?????????????????490?????????????????495

Leu?Ala?Leu?Leu?Ala?Gln?Arg?Lys?Ala?Pro?Leu?Val?Arg?Phe?Arg?Gly

500?????????????????505?????????????????510

Glu?Trp?Val?Ala?Val?Asp?Pro?Leu?Glu?Leu?Asp?Ala?Ile?Gln?Arg?His

515?????????????????520?????????????????525

Leu?Ala?Gln?Gly?Pro?Gly?Arg?Met?Ala?Leu?Ser?Glu?Ala?Val?Arg?Val

530?????????????????535?????????????????540

Ser?Leu?Leu?Gly?Glu?Thr?Arg?His?Gly?Gln?Leu?Pro?Val?Thr?Val?Leu

545?????????????????550?????????????????555?????????????????560

Ala?Thr?Gly?Ala?Leu?Glu?Glu?Arg?Leu?Arg?Leu?Leu?Arg?Glu?Gly?Gly

565?????????????????570?????????????????575

Ala?Thr?Ala?Gln?Asp?Ala?Pro?Arg?Ala?Leu?Arg?Ala?Thr?Leu?Arg?Pro

580?????????????????585?????????????????590

Tyr?Gln?Ser?Arg?Gly?Leu?His?Trp?Leu?Asp?Thr?Leu?Ala?Ser?Leu?Gly

595?????????????????600?????????????????605

Leu?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Val?Gln

610?????????????????615?????????????????620

Val?Leu?Ala?Phe?Leu?Leu?Arg?Arg?Leu?Glu?Gln?Ala?Pro?Asp?Glu?Ala

625?????????????????630?????????????????635?????????????????640

Arg?Pro?Thr?Leu?Leu?Val?Ala?Pro?Thr?Ser?Val?Val?Gly?Asn?Trp?Glu

645?????????????????650?????????????????655

Arg?Glu?Leu?Ala?Arg?Phe?Ala?Pro?Thr?Leu?Arg?Leu?Thr?Arg?His?Tyr

660?????????????????665?????????????????670

Gly?Ala?Glu?Arg?Ala?Arg?Ala?Ala?Asn?Arg?Phe?Pro?Arg?Ala?Pro?Gly

675?????????????????680?????????????????685

Ala?Val?Val?Leu?Thr?Thr?Tyr?Gly?Leu?Leu?Arg?Arg?Asp?Ala?Ala?Leu

690?????????????????695?????????????????700

Leu?Ala?Arg?Val?Asp?Trp?Gly?Ala?Val?Val?Leu?Asp?Glu?Ala?Gln?Asn

705?????????????????710?????????????????715?????????????????720

Ile?Lys?Asn?Ala?Ala?Ser?Ala?Thr?Ala?Arg?Ala?Ala?Arg?Ala?Leu?Arg

725?????????????????730?????????????????735

Ala?Ser?Gln?Arg?Phe?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu

740?????????????????745?????????????????750

Ala?Glu?Leu?Trp?Ser?Ile?Leu?Glu?Phe?Ala?Asn?Pro?Gly?Leu?Leu?Gly

755?????????????????760?????????????????765

Pro?Leu?Glu?Thr?Phe?Arg?Arg?Glu?Leu?Ala?Leu?Pro?Ile?Glu?Arg?His

770?????????????????775?????????????????780

Gly?Asn?Gln?Glu?Ala?Ser?Ala?Arg?Leu?Arg?Arg?Leu?Val?Ser?Pro?Phe

785?????????????????790?????????????????795?????????????????800

Val?Leu?Arg?Arg?Leu?Lys?Ser?Asp?Pro?Thr?Ile?Ile?Thr?Asp?Leu?Pro

805?????????????????810?????????????????815

Ala?Lys?Asn?Glu?Met?Lys?Val?Val?Cys?Thr?Leu?Thr?Arg?Glu?Gln?Ala

820?????????????????825?????????????????830

Ser?Leu?Tyr?Lys?Ala?Val?Val?Asp?Glu?Glu?Leu?Arg?Arg?Ile?Glu?Glu

835?????????????????840?????????????????845

Ala?Asp?Gly?Met?Glu?Arg?Arg?Gly?Arg?Val?Leu?Ala?Leu?Leu?Leu?Tyr

850?????????????????855?????????????????860

Thr?Lys?Gln?Ile?Ala?Asn?His?Pro?Ala?Gln?Tyr?Leu?Gly?Glu?Ser?Gly

865?????????????????870?????????????????875?????????????????880

Pro?Leu?Pro?Gly?Arg?Ser?Gly?Lys?Leu?Ala?Arg?Val?Val?Glu?Met?Leu

885?????????????????890?????????????????895

Glu?Glu?Ser?Leu?Ala?Ala?Gly?Asp?Lys?Ala?Leu?Val?Phe?Thr?Gln?Phe

900?????????????????905?????????????????910

Arg?Glu?Met?Gly?Asp?Lys?Leu?Val?Ala?His?Leu?Ser?Glu?Tyr?Leu?Gly

915?????????????????920?????????????????925

His?Glu?Val?Leu?Phe?Leu?His?Gly?Gly?Thr?Pro?Arg?Lys?Ala?Arg?Asp

930?????????????????935?????????????????940

Glu?Met?Val?Arg?Arg?Phe?Gln?Glu?Asp?Val?His?Gly?Pro?Arg?Val?Phe

945?????????????????950?????????????????955?????????????????960

Val?Leu?Ser?Val?Lys?Ala?Gly?Gly?Thr?Gly?Leu?Asn?Leu?Thr?Ala?Ala

965?????????????????970?????????????????975

Ser?His?Val?Phe?His?Tyr?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asp

980?????????????????985?????????????????990

Gln?Ala?Thr?Asp?Arg?Ala?Tyr?Arg??Ile?Gly?Gln?Thr?Arg??Ala?Val?Gln

995?????????????????1000?????????????????1005

Val?His??Lys?Leu?Val?Cys?Ala??Gly?Thr?Val?Glu?Glu??Lys?Val?Asp

1010?????????????????1015?????????????????1020

Arg?Leu??Leu?Glu?Gln?Lys?Arg??Gln?Leu?Ala?Glu?Lys??Val?Val?Gly

1025?????????????????1030?????????????????1035

Ala?Gly??Glu?His?Trp?Val?Thr??Glu?Leu?Asp?Thr?Thr??Ala?Leu?Arg

1040?????????????????1045?????????????????1050

Glu?Leu??Phe?Ser?Leu?Ser?Glu??Gly?Ala?Val?Ala?Asp??Asp?Gly?Asp

1055?????????????????1060?????????????????1065

Ala?Glu??Gly?Glu?Asp?Asp?Ala??Arg?Val?Arg?Ala?Pro??Arg?Arg?Arg

1070?????????????????1075?????????????????1080

Gly?Arg??Ala?Ser?Ala?Lys?Ala??Val?Ser?Arg

1085?????????????????1090

<210>55

<211>2871

<212>DNA

＜213〉nocardia farcinica

<400>55

atggtgggcg?ccggcggccc?gccgggtgtc?ggtgccacct?gcttggatgg?acggatgctg????60

cacggactgt?ggtcgccggg?ttccggcctg?gtgctgtgga?ccgagggcga?ggtgccgccc????120

gcgctgcccg?acccggccgg?tgcgttgctg?cgcgcatcgc?ggttccggca?tcgggcgcag????180

gtgctggtgc?cgggccccgc?cggcccacag?ctcacgcagg?tgcgcgcgca?cgccctggtg????240

ccacaggccg?cggtcgacgt?gctgcggcag?cggttacccg?tcgaatcggt?ggcgggtgac????300

ctgcgctttc?tcgctcacgt?cgccgacggg?atcgatcggt?gggtgcgggc?cggtcgcgtg????360

gtgcccgacc?tgcaccgggc?cgacggacag?tggtgggcgc?gctggcggct?ggtcggcggt????420

gcccggcagc?gggcctggct?ggccgaactc?gcggtggcga?tgcccgcggc?gctgcgggtg????480

gccgggcagc?ccgcggcggt?gctcgacgat?ctggtcaccg?agctgaccga?tccgatcgtg????540

cgcaccaggc?tcgccgacgc?gccggtgacg?cacccgctgg?tgcgcgcact?ggtgcgggac????600

cagccgctcg?agacgggtag?ccaccagctg?gccgaggtgc?tgcggcgctg?gcgcgagagc????660

ctcaccgtcg?acgagccgga?gctggtgttg?cggctgctgg?aaccggacgg?ggagaccggt????720

atcgacgggg?acggcgggga?cgaccgggac?gacaccgtgg?cgctgtggcg?gctggaggtc????780

tgcctccgca?ccgagggcga?ggccccggcc?ccggtgccgg?cgaccgccga?cccgaacctg????840

ctgcgcatcg?ccgtcgagca?gctcggccgg?gcgcagcggg?cctacccccg?gctgcgcgat????900

ctgcccggcg?atccgcacag?cctcgacctg?ctgttgccca?ccgaggtggt?ggccgatctc????960

gtcgcgcacg?gtgcgcaggc?gttgcgcgag?gcgggggtgc?ggctgctgct?gccgcgcgcc???1020

tggaccatcg?ccgaacccac?cctgcggctc?gcggtgagca?gcgccgcgcc?cgccgcggag???1080

agcaccgtgg?gcatgcaggg?tctgctgtcc?tatcggtggg?aactggcggt?cggcgacaag???1140

gtgctcaccc?gcgccgagat?ggagcgcctg?gtccgcgcca?aatccgacct?ggtgcagttg???1200

cgcggggaat?gggtgcaggc?cgaccacaag?gtgctcgccg?ccgccgcccg?ctacgtcgcc???1260

gcgcatctgg?acacgtcgcc?ggtcaccctc?gccgacctgc?tcggcgagat?cgccgccacc???1320

cgcgtcgaca?aggtgccgct?caccgaggtc?accgccaccg?gctgggcggg?cgagttgttc???1380

gacggcggcc?gcgagccggt?ggcgaccccg?ggtgggctga?aggcgcagct?gcgcccgtat????1440

cagctgcgcg?gcctgagctg?gctggcgacg?atgagccgga?tgggctgcgg?cggcatcctc????1500

gccgacgaca?tgggtctcgg?caagacggtg?caggtgctgg?ccctgctggt?gcacgagcgc????1560

gagaccagca?cggcaccgcc?cggcccgaca?ctgctggtgt?gcccgatgtc?ggtggtcggc????1620

aactggcagc?gcgaggcgca?gcggttcgcc?cccgggctgc?gggtgctggt?gcaccacggc????1680

gccgaccgcc?gtcgcgacgc?cgaactcgat?gccgcggtgg?cggattcgga?cctggtgctc????1740

accacctacg?ccatcctggc?cagggatgcg?gccgaactgt?cgcgccagtc?gtgggaccgg????1800

gtggtgctcg?acgaggcgca?gcacatcaag?aacgccgcga?ccaggcaggc?acgtgccgcc????1860

cgtgccctgc?cggcccggca?tcgcctggcg?ctcaccggaa?ccccggtgga?gaaccggctc????1920

gaagagttgc?gctcgatcat?ggatttcgcg?gtgcccaagc?tgctcggtac?cgcaccgacc????1980

ttccgcgccc?ggttcgccgt?ccccatcgaa?cgcgggcagg?atcccaacgc?cctgtcccgc????2040

ctgcgcttcc?tcacccaacc?gttcgtgctg?cgccgggtca?aggccgatcc?ggcggtcatc????2100

ggcgatctgc?ccgacaagct?cgagatgacg?gtgcgggcga?acctgaccgt?cgagcaggcc????2160

gccctgtacc?aagccgtcgt?cgacgacatg?ctggtgaaac?tgcgcagtgc?caagggcatg????2220

gcccgcaagg?gtgcggtgct?cggcgcgctc?acccggctca?agcaggtgtg?caaccatccc????2280

gcgcacttcc?tcggtgacgg?ttccccggtg?ctgcatcgcg?gcaggcaccg?ctccggcaag????2340

ctcgccttgg?tcgaggacgt?gctcgacacc?gtcgtcgcgg?acggggagaa?ggcgttgctg????2400

ttcacccagt?tccgtgagtt?cggcgacctg?ctcgcgccct?atctgtccga?gcggttcggc????2460

gcgccgatcc?cgttcctgca?cggcggcgtg?accaagaaga?accgggacac?gatggtcgag????2520

cgcttccagt?ccggcgacgg?cccgccggtc?atgctgctgt?ccctcaaggc?cggcggcacc????2580

gggctcaccc?tcaccgccgc?caatcacgtg?gtgcacctgg?atcgctggtg?gaatccggcg????2640

gtggagaacc?aggccaccga?tcgcgccttc?cgcatcggcc?agcgccgcga?cgtccaggtg????2700

cgcaagctgg?tctgcgtcga?caccatcgag?gaacggatcg?acgagatgat?caccggcaag??2760

agcaggctcg?cggacctggc?cgtggacgcg?ggggagaact?ggatcaccga?gctgggcacc??2820

gaggagctgc?gcgagttgtt?caccctcggc?gccgaggcgg?tgggggagtg?a???????????2871

<210>56

<211>956

<212>PRT

＜213〉nocardia farcinica

<400>56

Met?Val?Gly?Ala?Gly?Gly?Pro?Pro?Gly?Val?Gly?Ala?Thr?Cys?Leu?Asp

1???????????????5???????????????????10??????????????????15

Gly?Arg?Met?Leu?His?Gly?Leu?Trp?Ser?Pro?Gly?Ser?Gly?Leu?Val?Leu

20??????????????????25??????????????????30

Trp?Thr?Glu?Gly?Glu?Val?Pro?Pro?Ala?Leu?Pro?Asp?Pro?Ala?Gly?Ala

35??????????????????40??????????????????45

Leu?Leu?Arg?Ala?Ser?Arg?Phe?Arg?His?Arg?Ala?Gln?Val?Leu?Val?Pro

50??????????????????55??????????????????60

Gly?Pro?Ala?Gly?Pro?Gln?Leu?Thr?Gln?Val?Arg?Ala?His?Ala?Leu?Val

65??????????????????70??????????????????75??????????????????80

Pro?Gln?Ala?Ala?Val?Asp?Val?Leu?Arg?Gln?Arg?Leu?Pro?Val?Glu?Ser

85??????????????????90??????????????????95

Val?Ala?Gly?Asp?Leu?Arg?Phe?Leu?Ala?His?Val?Ala?Asp?Gly?Ile?Asp

100?????????????????105?????????????????110

Arg?Trp?Val?Arg?Ala?Gly?Arg?Val?Val?Pro?Asp?Leu?His?Arg?Ala?Asp

115?????????????????120?????????????????125

Gly?Gln?Trp?Trp?Ala?Arg?Trp?Arg?Leu?Val?Gly?Gly?Ala?Arg?Gln?Arg

130?????????????????135?????????????????140

Ala?Trp?Leu?Ala?Glu?Leu?Ala?Val?Ala?Met?Pro?Ala?Ala?Leu?Arg?Val

145?????????????????150?????????????????155?????????????????160

Ala?Gly?Gln?Pro?Ala?Ala?Val?Leu?Asp?Asp?Leu?Val?Thr?Glu?Leu?Thr

165?????????????????170?????????????????175

Asp?Pro?Ile?Val?Arg?Thr?Arg?Leu?Ala?Asp?Ala?Pro?Val?Thr?His?Pro

180?????????????????185?????????????????190

Leu?Val?Arg?Ala?Leu?Val?Arg?Asp?Gln?Pro?Leu?Glu?Thr?Gly?Ser?His

195?????????????????200?????????????????205

Gln?Leu?Ala?Glu?Val?Leu?Arg?Arg?Trp?Arg?Glu?Ser?Leu?Thr?Val?Asp

210?????????????????215?????????????????220

Glu?Pro?Glu?Leu?Val?Leu?Arg?Leu?Leu?Glu?Pro?Asp?Gly?Glu?Thr?Gly

225?????????????????230?????????????????235?????????????????240

Ile?Asp?Gly?Asp?Gly?Gly?Asp?Asp?Arg?Asp?Asp?Thr?Val?Ala?Leu?Trp

245?????????????????250?????????????????255

Arg?Leu?Glu?Val?Cys?Leu?Arg?Thr?Glu?Gly?Glu?Ala?Pro?Ala?Pro?Val

260?????????????????265?????????????????270

Pro?Ala?Thr?Ala?Asp?Pro?Asn?Leu?Leu?Arg?Ile?Ala?Val?Glu?Gln?Leu

275?????????????????280?????????????????285

Gly?Arg?Ala?Gln?Arg?Ala?Tyr?Pro?Arg?Leu?Arg?Asp?Leu?Pro?Gly?Asp

290?????????????????295?????????????????300

Pro?His?Ser?Leu?Asp?Leu?Leu?Leu?Pro?Thr?Glu?Val?Val?Ala?Asp?Leu

305?????????????????310?????????????????315?????????????????320

Val?Ala?His?Gly?Ala?Gln?Ala?Leu?Arg?Glu?Ala?Gly?Val?Arg?Leu?Leu

325?????????????????330?????????????????335

Leu?Pro?Arg?Ala?Trp?Thr?Ile?Ala?Glu?Pro?Thr?Leu?Arg?Leu?Ala?Val

340?????????????????345?????????????????350

Ser?Ser?Ala?Ala?Pro?Ala?Ala?Glu?Ser?Thr?Val?Gly?Met?Gln?Gly?Leu

355?????????????????360?????????????????365

Leu?Ser?Tyr?Arg?Trp?Glu?Leu?Ala?Val?Gly?Asp?Lys?Val?Leu?Thr?Arg

370?????????????????375?????????????????380

Ala?Glu?Met?Glu?Arg?Leu?Val?Arg?Ala?Lys?Ser?Asp?Leu?Val?Gln?Leu

385?????????????????390?????????????????395?????????????????400

Arg?Gly?Glu?Trp?Val?Gln?Ala?Asp?His?Lys?Val?Leu?Ala?Ala?Ala?Ala

405?????????????????410?????????????????415

Arg?Tyr?Val?Ala?Ala?His?Leu?Asp?Thr?Ser?Pro?Val?Thr?Leu?Ala?Asp

420?????????????????425?????????????????430

Leu?Leu?Gly?Glu?Ile?Ala?Ala?Thr?Arg?Val?Asp?Lys?Val?Pro?Leu?Thr

435?????????????????440?????????????????445

Glu?Val?Thr?Ala?Thr?Gly?Trp?Ala?Gly?Glu?Leu?Phe?Asp?Gly?Gly?Arg

450?????????????????455?????????????????460

Glu?Pro?Val?Ala?Thr?Pro?Gly?Gly?Leu?Lys?Ala?Gln?Leu?Arg?Pro?Tyr

465?????????????????470?????????????????475?????????????????480

Gln?Leu?Arg?Gly?Leu?Ser?Trp?Leu?Ala?Thr?Met?Ser?Arg?Met?Gly?Cys

485?????????????????490?????????????????495

Gly?Gly?Ile?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Val?Gln?Val

500?????????????????505?????????????????510

Leu?Ala?Leu?Leu?Val?His?Glu?Arg?Glu?Thr?Ser?Thr?Ala?Pro?Pro?Gly

515?????????????????520?????????????????525

Pro?Thr?Leu?Leu?Val?Cys?Pro?Met?Ser?Val?Val?Gly?Asn?Trp?Gln?Arg

530?????????????????535?????????????????540

Glu?Ala?Gln?Arg?Phe?Ala?Pro?Gly?Leu?Arg?Val?Leu?Val?His?His?Gly

545?????????????????550?????????????????555?????????????????560

Ala?Asp?Arg?Arg?Arg?Asp?Ala?Glu?Leu?Asp?Ala?Ala?Val?Ala?Asp?Ser

565?????????????????570?????????????????575

Asp?Leu?Val?Leu?Thr?Thr?Tyr?Ala?Ile?Leu?Ala?Arg?Asp?Ala?Ala?Glu

580?????????????????585?????????????????590

Leu?Ser?Arg?Gln?Ser?Trp?Asp?Arg?Val?Val?Leu?Asp?Glu?Ala?Gln?His

595?????????????????600?????????????????605

Ile?Lys?Asn?Ala?Ala?Thr?Arg?Gln?Ala?Arg?Ala?Ala?Arg?Ala?Leu?Pro

610?????????????????615?????????????????620

Ala?Arg?His?Arg?Leu?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu

625?????????????????630?????????????????635?????????????????640

Glu?Glu?Leu?Arg?Ser?Ile?Met?Asp?Phe?Ala?Val?Pro?Lys?Leu?Leu?Gly

645?????????????????650?????????????????655

Thr?Ala?Pro?Thr?Phe?Arg?Ala?Arg?Phe?Ala?Val?Pro?Ile?Glu?Arg?Gly

660?????????????????665?????????????????670

Gln?Asp?Pro?Asn?Ala?Leu?Ser?Arg?Leu?Arg?Phe?Leu?Thr?Gln?Pro?Phe

675?????????????????680?????????????????685

Val?Leu?Arg?Arg?Val?Lys?Ala?Asp?Pro?Ala?Val?Ile?Gly?Asp?Leu?Pro

690?????????????????695?????????????????700

Asp?Lys?Leu?Glu?Met?Thr?Val?Arg?Ala?Asn?Leu?Thr?Val?Glu?Gln?Ala

705?????????????????710?????????????????715?????????????????720

Ala?Leu?Tyr?Gln?Ala?Val?Val?Asp?Asp?Met?Leu?Val?Lys?Leu?Arg?Ser

725?????????????????730?????????????????735

Ala?Lys?Gly?Met?Ala?Arg?Lys?Gly?Ala?Val?Leu?Gly?Ala?Leu?Thr?Arg

740?????????????????745?????????????????750

Leu?Lys?Gln?Val?Cys?Asn?His?Pro?Ala?His?Phe?Leu?Gly?Asp?Gly?Ser

755?????????????????760?????????????????765

Pro?Val?Leu?His?Arg?Gly?Arg?His?Arg?Ser?Gly?Lys?Leu?Ala?Leu?Val

770?????????????????775?????????????????780

Glu?Asp?Val?Leu?Asp?Thr?Val?Val?Ala?Asp?Gly?Glu?Lys?Ala?Leu?Leu

785?????????????????790?????????????????795?????????????????800

Phe?Thr?Gln?Phe?Arg?Glu?Phe?Gly?Asp?Leu?Leu?Ala?Pro?Tyr?Leu?Ser

805?????????????????810?????????????????815

Glu?Arg?Phe?Gly?Ala?Pro?Ile?Pro?Phe?Leu?His?Gly?Gly?Val?Thr?Lys

820?????????????????825?????????????????830

Lys?Asn?Arg?Asp?Thr?Met?Val?Glu?Arg?Phe?Gln?Ser?Gly?Asp?Gly?Pro

835?????????????????840?????????????????845

Pro?Val?Met?Leu?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Thr?Gly?Leu?Thr?Leu

850?????????????????855?????????????????860

Thr?Ala?Ala?Asn?His?Val?Val?His?Leu?Asp?Arg?Trp?Trp?Asn?Pro?Ala

865?????????????????870?????????????????875?????????????????880

Val?Glu?Asn?Gln?Ala?Thr?Asp?Arg?Ala?Phe?Arg?Ile?Gly?Gln?Arg?Arg

885?????????????????890?????????????????895

Asp?Val?Gln?Val?Arg?Lys?Leu?Val?Cys?Val?Asp?Thr?Ile?Glu?Glu?Arg

900?????????????????905?????????????????910

Ile?Asp?Glu?Met?Ile?Thr?Gly?Lys?Ser?Arg?Leu?Ala?Asp?Leu?Ala?Val

915?????????????????920?????????????????925

Asp?Ala?Gly?Glu?Asn?Trp?Ile?Thr?Glu?Leu?Gly?Thr?Glu?Glu?Leu?Arg

930?????????????????935?????????????????940

Glu?Leu?Phe?Thr?Leu?Gly?Ala?Glu?Ala?Val?Gly?Glu

945?????????????????950?????????????????955

<210>57

<211>3264

<212>DNA

＜213〉produce foam joint ball cyanobacteria

<400>57

atggcaattt?tacacggtaa?ttggttagta?agaaatcaaa?atggttgttt?atttatttgg?????60

ggtgaaactt?ggcgttcatc?acgagtcgat?tttgctctga?atgtatctca?agatatacca????120

ctacatccat?tggtaatgtc?accaattgat?ttgagtgagt?tgttaagtta?tcataatatc????180

aaaattccta?gcttaataca?gcaatcccaa?gttgctttat?ctggcactgg?gcgaactcgt????240

aaaagtacaa?gtactactaa?atttagctgg?acaactcact?ctctaatcat?tgatttacca????300

actcatatct?cagaaaataa?tccccaagaa?atagaattta?tttccccttt?gcattctgct????360

actttgggtt?ctgaaataaa?ttcaccccaa?tatctccaac?cgtggcgagt?cgagggtttt????420

tgtctcaacc?ccactgaagc?gataaaattt?ctcgctgctg?ttcctttaaa?tgctgctaga????480

gaagaagata?ctttgttcgg?tggagattta?cgtttttggt?cacaaattgc?ccgttggagt????540

ttggatttaa?tctctcggtg?taagtttttg?ccaactattc?aaagacagtt?tgatagttct????600

attgttgcta?ggtggcaagt?gcttttagac?agtgcaatag?atggaacacg?cctggaaaaa????660

ttttctgcaa?aaatgccatt?agcttgtcgt?acttatcgga?agggaatggg?gagtggggag????720

tggggagtgg?ggagtgggga?ggaatcttcc?ccatccataa?tgtatgtaga?ttttccaact????780

gaaccccagg?aactattatt?aggatttctc?aacagtacca?tagatgccca?agtgcgagaa????840

atgttagctt?ctcaacctct?actagaaact?agagtgatgg?catctttacc?atctgcggtg????900

cgacagtggt?tgcaaggttt?aaccagtgca?tctcacacag?tgaatgcaga?tgcaatggaa????960

gtagaaagat?tagaagcagc?cctgaaatct?tggactatgc?cgttgcaata?tcaactggta???1020

ggaaaaccct?cgtttcgcgc?ctgttttcaa?ctgcttcccc?ctgcttctgg?ggcaacagat???1080

tggatattgg?catattttct?ccaagctgcg?gatgatgaaa?atttattagt?ggatgcggca???1140

actatttggc?atcacccagt?tgaacaatta?gtttatcaaa?atcgcaccat?tgatcaaccc???1200

caagaaactt?tattgcgggg?cttgggttta?gcttcgcgat?tatatccagt?tcttacaccg???1260

agtttagaaa?cagaatatcc?ccaatgttgt?cgcctcaacc?cattacaagc?ttatgaattt???1320

atcaagtctg?tagcttggcg?atttgaagat?agtggtttgg?gggtaatttt?acctcctagt???1380

ttgactaacc?gcgaaggatg?ggcgaaccgt?ttggggttaa?aaattagtgc?tgaaactcaa???1440

aagaaaaaac?agggacgctt?gggtttacaa?agtttactga?attttcaatg?gcaattggca????1500

attggtggac?aaacaatttc?taaaaccgag?tttaataaac?tggtagcttt?aaatagccca????1560

ctggtagaaa?ttaacggcga?atgggtggaa?ttgcgacccc?aggatattaa?aacagcacag????1620

acattttttg?cttctcgtaa?agacgaaatg?acgctttctt?tggaagatgc?tttacgcctc????1680

agttctggcg?atacccaagc?gattgaaaag?ttacctgtgg?tcagttttga?agcatctggg????1740

acattgcaag?agttaattgg?ggcgttaacc?aataatcaag?ccatttcacc?cctcccaaca????1800

cctgcaaatt?ttcaaggaca?gttacgacct?tatcaagaaa?gaggggcggc?ttggctggct????1860

ttcttagaac?gttggggttt?aggtgcttgt?ttggctgatg?atatggggct?gggaaaaaca????1920

attcagttaa?ttgccttttt?actgcacctc?aaagaacaag?acgcactgga?aaatcccaca????1980

ttacttgttt?gtccgacttc?tattttaggt?aactgggaac?gggaaattaa?aaaatttgct????2040

cctactctca?aagttttaca?gcaccacggc?gataaacgtc?tcaaaggtaa?agcgtttgta????2100

gaagcagtca?aaaaacacga?tgtaattatt?accagttact?cactcgttca?ccgggatatt????2160

aaatctttgc?agagtgtcga?ttggcaaaca?gttgtattag?atgaagccca?gaatgtgaaa????2220

aatcctgaag?ctaaacaatc?gcaggctgtg?aggggattaa?aaactacatt?tcgcatagct????2280

ttaacaggga?caccagtaga?aaacaaactg?caagaattgt?ggtctatttt?agattttctt????2340

aatcctgggt?atttgggaaa?tcgtcaattt?ttccagagac?ggtttgctat?gccaattgaa????2400

aagtatggtg?atacagcatc?tttaaatcaa?ttgcggggtt?tagttcaacc?gtttattcta????2460

cgtcgtctga?aaacagatcg?tgatattatt?caagatttgc?cagaaaagca?agaaatgacg????2520

gttttttgtg?ggcttgcggc?tgaacaagct?gcactttatc?aacaagtagt?tgaagcatct????2580

ttagtagaaa?ttgaatctgc?tgagggtttg?caacgtcgag?ggatgatttt?agctttactt????2640

gtgaaactta?aacaaatctg?taatcatcea?gcccaatatt?tgaaagccgc?gacattacaa????2700

gaacatagtt?ctgctaaact?gcaacggcta?gatgaaatgt?taacggtagc?tttggaggaa????2760

ggagataggg?ctttaatttt?cactcaattt?gctgaatggg?gtaagttatt?aaaagctcat??2820

ttacaacaaa?cacttgggaa?agaaatattc?tttttatatg?gtggtagcag?taaaaaacaa??2880

cgcgaggaaa?tgattgaccg?tttccaacat?gacccccaag?gacctccgat?tatgattctt??2940

tctttaaaag?cgggtggggt?aggcttgaat?ttaaccaggg?ctaatcatgt?atttcacttt??3000

gatagatggt?ggaatcccgc?agtggaaaat?caagcgacag?atagagtatt?tcgtattggt??3060

caaacccgga?atgtgcaagt?gcataaattt?gtctgtactg?gcacattaga?agaaaaaatt??3120

catgacatga?ttgaaagtaa?aaaacaatta?gcggaacaag?tagttggtgc?tggtgaggag??3180

tggctgactg?aaatgaatac?tgaccaattg?cgtgatttac?tcattcttga?tcgcagtgcc??3240

ataattgatg?aggatgaagt?ttaa?????????????????????????????????????????3264

<210>58

<211>1087

<212>PRT

＜213〉produce foam joint ball cyanobacteria

<400>58

Met?Ala?Ile?Leu?His?Gly?Asn?Trp?Leu?Val?Arg?Asn?Gln?Asn?Gly?Cys

1???????????????5???????????????????10??????????????????15

Leu?Phe?Ile?Trp?Gly?Glu?Thr?Trp?Arg?Ser?Ser?Arg?Val?Asp?Phe?Ala

20??????????????????25??????????????????30

Leu?Asn?Val?Ser?Gln?Asp?Ile?Pro?Leu?His?Pro?Leu?Val?Met?Ser?Pro

35??????????????????40??????????????????45

Ile?Asp?Leu?Ser?Glu?Leu?Leu?Ser?Tyr?His?Asn?Ile?Lys?Ile?Pro?Ser

50??????????????????55??????????????????60

Leu?Ile?Gln?Gln?Ser?Gln?Val?Ala?Leu?Ser?Gly?Thr?Gly?Arg?Thr?Arg

65??????????????????70??????????????????75??????????????????80

Lys?Ser?Thr?Ser?Thr?Thr?Lys?Phe?Ser?Trp?Thr?Thr?His?Ser?Leu?Ile

85??????????????????90??????????????????95

Ile?Asp?Leu?Pro?Thr?His?Ile?Ser?Glu?Asn?Asn?Pro?Gln?Glu?Ile?Glu

100?????????????????105?????????????????110

Phe?Ile?Ser?Pro?Leu?His?Ser?Ala?Thr?Leu?Gly?Ser?Glu?Ile?Asn?Ser

115?????????????????120?????????????????125

Pro?Gln?Tyr?Leu?Gln?Pro?Trp?Arg?Val?Glu?Gly?Phe?Cys?Leu?Asn?Pro

130?????????????????135?????????????????140

Thr?Glu?Ala?Ile?Lys?Phe?Leu?Ala?Ala?Val?Pro?Leu?Asn?Ala?Ala?Arg

145?????????????????150?????????????????155?????????????????160

Glu?Glu?Asp?Thr?Leu?Phe?Gly?Gly?Asp?Leu?Arg?Phe?Trp?Ser?Gln?Ile

165?????????????????170?????????????????175

Ala?Arg?Trp?Ser?Leu?Asp?Leu?Ile?Ser?Arg?Cys?Lys?Phe?Leu?Pro?Thr

180?????????????????185?????????????????190

Ile?Gln?Arg?Gln?Phe?Asp?Ser?Ser?Ile?Val?Ala?Arg?Trp?Gln?Val?Leu

195?????????????????200?????????????????205

Leu?Asp?Ser?Ala?Ile?Asp?Gly?Thr?Arg?Leu?Glu?Lys?Phe?Ser?Ala?Lys

210?????????????????215?????????????????220

Met?Pro?Leu?Ala?Cys?Arg?Thr?Tyr?Arg?Lys?Gly?Met?Gly?Ser?Gly?Glu

225?????????????????230?????????????????235?????????????????240

Trp?Gly?Val?Gly?Ser?Gly?Glu?Glu?Ser?Ser?Pro?Ser?Ile?Met?Tyr?Val

245?????????????????250?????????????????255

Asp?Phe?Pro?Thr?Glu?Pro?Gln?Glu?Leu?Leu?Leu?Gly?Phe?Leu?Asn?Ser

260?????????????????265?????????????????270

Thr?Ile?Asp?Ala?Gln?Val?Arg?Glu?Met?Leu?Ala?Ser?Gln?Pro?Leu?Leu

275?????????????????280?????????????????285

Glu?Thr?Arg?Val?Met?Ala?Ser?Leu?Pro?Ser?Ala?Val?Arg?Gln?Trp?Leu

290?????????????????295?????????????????300

Gln?Gly?Leu?Thr?Ser?Ala?Ser?His?Thr?Val?Asn?Ala?Asp?Ala?Met?Glu

305?????????????????310?????????????????315?????????????????320

Val?Glu?Arg?Leu?Glu?Ala?Ala?Leu?Lys?Ser?Trp?Thr?Met?Pro?Leu?Gln

325?????????????????330?????????????????335

Tyr?Gln?Leu?Val?Gly?Lys?Pro?Ser?Phe?Arg?Ala?Cys?Phe?Gln?Leu?Leu

340?????????????????345?????????????????350

Pro?Pro?Ala?Ser?Gly?Ala?Thr?Asp?Trp?Ile?Leu?Ala?Tyr?Phe?Leu?Gln

355?????????????????360?????????????????365

Ala?Ala?Asp?Asp?Glu?Asn?Leu?Leu?Val?Asp?Ala?Ala?Thr?Ile?Trp?His

370?????????????????375?????????????????380

His?Pro?Val?Glu?Gln?Leu?Val?Tyr?Gln?Asn?Arg?Thr?Ile?Asp?Gln?Pro

385?????????????????390?????????????????395?????????????????400

Gln?Glu?Thr?Leu?Leu?Arg?Gly?Leu?Gly?Leu?Ala?Ser?Arg?Leu?Tyr?Pro

405?????????????????410?????????????????415

Val?Leu?Thr?Pro?Ser?Leu?Glu?Thr?Glu?Tyr?Pro?Gln?Cys?Cys?Arg?Leu

420?????????????????425?????????????????430

Asn?Pro?Leu?Gln?Ala?Tyr?Glu?Phe?Ile?Lys?Ser?Val?Ala?Trp?Arg?Phe

435?????????????????440?????????????????445

Glu?Asp?Ser?Gly?Leu?Gly?Val?Ile?Leu?Pro?Pro?Ser?Leu?Thr?Asn?Arg

450?????????????????455?????????????????460

Glu?Gly?Trp?Ala?Asn?Arg?Leu?Gly?Leu?Lys?Ile?Ser?Ala?Glu?Thr?Gln

465?????????????????470?????????????????475?????????????????480

Lys?Lys?Lys?Gln?Gly?Arg?Leu?Gly?Leu?Gln?Ser?Leu?Leu?Asn?Phe?Gln

485?????????????????490?????????????????495

Trp?Gln?Leu?Ala?Ile?Gly?Gly?Gln?Thr?Ile?Ser?Lys?Thr?Glu?Phe?Asn

500?????????????????505?????????????????510

Lys?Leu?Val?Ala?Leu?Asn?Ser?Pro?Leu?Val?Glu?Ile?Asn?Gly?Glu?Trp

515?????????????????520?????????????????525

Val?Glu?Leu?Arg?Pro?Gln?Asp?Ile?Lys?Thr?Ala?Gln?Thr?Phe?Phe?Ala

530?????????????????535?????????????????540

Ser?Arg?Lys?Asp?Glu?Met?Thr?Leu?Ser?Leu?Glu?Asp?Ala?Leu?Arg?Leu

545?????????????????550?????????????????555?????????????????560

Ser?Ser?Gly?Asp?Thr?Gln?Ala?Ile?Glu?Lys?Leu?Pro?Val?Val?Ser?Phe

565?????????????????570?????????????????575

Glu?Ala?Ser?Gly?Thr?Leu?Gln?Glu?Leu?Ile?Gly?Ala?Leu?Thr?Asn?Asn

580?????????????????585?????????????????590

Gln?Ala?Ile?Ser?Pro?Leu?Pro?Thr?Pro?Ala?Asn?Phe?Gln?Gly?Gln?Leu

595?????????????????600?????????????????605

Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Ala?Ala?Trp?Leu?Ala?Phe?Leu?Glu?Arg

610?????????????????615?????????????????620

Trp?Gly?Leu?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr

625?????????????????630?????????????????635?????????????????640

Ile?Gln?Leu?Ile?Ala?Phe?Leu?Leu?His?Leu?Lys?Glu?Gln?Asp?Ala?Leu

645?????????????????650?????????????????655

Glu?Asn?Pro?Thr?Leu?Leu?Val?Cys?Pro?Thr?Ser?Ile?Leu?Gly?Asn?Trp

660?????????????????665?????????????????670

Glu?Arg?Glu?Ile?Lys?Lys?Phe?Ala?Pro?Thr?Leu?Lys?Val?Leu?Gln?His

675?????????????????680?????????????????685

His?Gly?Asp?Lys?Arg?Leu?Lys?Gly?Lys?Ala?Phe?Val?Glu?Ala?Val?Lys

690?????????????????695?????????????????700

Lys?His?Asp?Val?Ile?Ile?Thr?Ser?Tyr?Ser?Leu?Val?His?Arg?Asp?Ile

705?????????????????710?????????????????715?????????????????720

Lys?Ser?Leu?Gln?Ser?Val?Asp?Trp?Gln?Thr?Val?Val?Leu?Asp?Glu?Ala

725?????????????????730?????????????????735

Gln?Asn?Val?Lys?Asn?Pro?Glu?Ala?Lys?Gln?Ser?Gln?Ala?Val?Arg?Gly

740?????????????????745?????????????????750

Leu?Lys?Thr?Thr?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn

755?????????????????760?????????????????765

Lys?Leu?Gln?Glu?Leu?Trp?Ser?Ile?Leu?Asp?Phe?Leu?Asn?Pro?Gly?Tyr

770?????????????????775?????????????????780

Leu?Gly?Asn?Arg?Gln?Phe?Phe?Gln?Arg?Arg?Phe?Ala?Met?Pro?Ile?Glu

785?????????????????790?????????????????795?????????????????800

Lys?Tyr?Gly?Asp?Thr?Ala?Ser?Leu?Asn?Gln?Leu?Arg?Gly?Leu?Val?Gln

805?????????????????810?????????????????815

Pro?Phe?Ile?Leu?Arg?Arg?Leu?Lys?Thr?Asp?Arg?Asp?Ile?Ile?Gln?Asp

820?????????????????825?????????????????830

Leu?Pro?Glu?Lys?Gln?Glu?Met?Thr?Val?Phe?Cys?Gly?Leu?Ala?Ala?Glu

835?????????????????840?????????????????845

Gln?Ala?Ala?Leu?Tyr?Gln?Gln?Val?Val?Glu?Ala?Ser?Leu?Val?Glu?Ile

850?????????????????855?????????????????860

Glu?Ser?Ala?Glu?Gly?Leu?Gln?Arg?Arg?Gly?Met?Ile?Leu?Ala?Leu?Leu

865?????????????????870?????????????????875?????????????????880

Val?Lys?Leu?Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Gln?Tyr?Leu?Lys?Ala

885?????????????????890?????????????????895

Ala?Thr?Leu?Gln?Glu?His?Ser?Ser?Ala?Lys?Leu?Gln?Arg?Leu?Asp?Glu

900?????????????????905?????????????????910

Met?Leu?Thr?Val?Ala?Leu?Glu?Glu?Gly?Asp?Arg?Ala?Leu?Ile?Phe?Thr

915?????????????????920?????????????????925

Gln?Phe?Ala?Glu?Trp?Gly?Lys?Leu?Leu?Lys?Ala?His?Leu?Gln?Gln?Thr

930?????????????????935?????????????????940

Leu?Gly?Lys?Glu?Ile?Phe?Phe?Leu?Tyr?Gly?Gly?Ser?Ser?Lys?Lys?Gln

945?????????????????950?????????????????955?????????????????960

Arg?Glu?Glu?Met?Ile?Asp?Arg?Phe?Gln?His?Asp?Pro?Gln?Gly?Pro?Pro

965?????????????????970?????????????????975

Ile?Met?Ile?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr

980?????????????????985?????????????????990

Arg?Ala?Asn?His?Val?Phe?His?Phe??Asp?Arg?Trp?Trp?Asn??Pro?Ala?Val

995?????????????????1000?????????????????1005

Glu?Asn??Gln?Ala?Thr?Asp?Arg??Val?Phe?Arg?Ile?Gly??Gln?Thr?Arg

1010?????????????????1015?????????????????1020

Asn?Val??Gln?Val?His?Lys?Phe??Val?Cys?Thr?Gly?Thr??Leu?Glu?Glu

1025?????????????????1030?????????????????1035

Lys?Ile??His?Asp?Met?Ile?Glu??Ser?Lys?Lys?Gln?Leu??Ala?Glu?Gln

1040?????????????????1045?????????????????1050

Val?Val??Gly?Ala?Gly?Glu?Glu??Trp?Leu?Thr?Glu?Met??Asn?Thr?Asp

1055?????????????????1060?????????????????1065

Gln?Leu??Arg?Asp?Leu?Leu?Ile??Leu?Asp?Arg?Ser?Ala??Ile?Ile?Asp

1070?????????????????1075?????????????????1080

Glu?Asp??Glu?Val

1085

<210>59

<211>3228

<212>DNA

＜213〉beads algae

<400>59

atggcaattc?tacacggtag?ttggatatta?aatgagcagg?agagttgttt?atttatttgg?????60

ggggaaactt?ggcgatcgcc?acaagtggat?tttaattttg?cggagatatc?cctcaatccc????120

ttggcgctgt?ctgcactgga?attaagtgag?tggttgcagt?ctcaacatca?ggcgatcgct????180

aagttgttac?cgcaacaatt?ggaaaaacga?acctccaaag?cagcaagttc?tgtaaaaata????240

aatttattaa?ctcattcaca?aataattgcc?ctgccaacgg?aaatttccca?acctcgtaaa????300

aaagaaacca?ttttaatttc?tcctgtgcat?tctgccgctt?tagcatctga?gtcagactct????360

gaagtttatt?tacaaacttg?gcgtgtagaa?ggtttttgtc?ttcctcctag?tgcagcaatt????420

aaattgctaa?cttctttacc?tttaaatata?actagtgggg?agaatgcttt?tttaggtgga????480

gatttacgtt?tctggtcaca?aattgcccgt?tggagtttag?atttaatttc?taggtctaag????540

tttctcccaa?ttatccaacg?acaacctaat?aattctgtaa?gtgctaaatg?gcaagtactt????600

ttagatagtg?ccgtagatgg?aactcgttta?gaaaagtttg?ctgcgaagat?gcccttggtt????660

tgtcggactt?atcaagaaat?tgggagtggg?gaatctccta?tatatataga?ttttcctagt????720

cagccgcagg?atttaatctt?gggttttctc?aatagtgcga?tagatacgca?attgcgggag????780

atggtgggga?atcagcctgt?ggtggaaact?cggttgatgg?catctttacc?atcggcggtg????840

cgacagtggt?tgcaagcgtt?aattgctgca?tctaattcaa?ttgatgcaga?tgctgttggt????900

ttagaaaggc?tggaagcggc?gctcaaggct?tggacgatgc?cgctacaata?tcaactagca????960

agtaaaaatc?aatttcgcac?ttgttttgaa?ttacgttctc?cagaaccaga?cgaaactgaa???1020

tggacgctgg?cgtatttcct?gcaagcagcc?gatgatccag?aatttttagt?agatgcggcg???1080

actatttggc?aaaatcctgt?tgaacagcta?atttatcaac?agcgaacgat?tgaagaaccc???1140

caggaaacgt?ttttgcgagg?tttggggtta?gcttctcgat?tgtatccggt?cattgccccc???1200

actttagata?cagaatcacc?ccaattttgt?catctcaagc?ccatgcaggc?ttatgaattt???1260

atcaaggctg?tggcttggcg?atttgaagat?agcggcttag?gggtgatttt?acctcctagt???1320

ttggcgaatc?gtgaaggctg?ggcaaatcgc?ttgggtttga?aaatctccgc?cgaaacgccg????1380

aagaaaaaac?caggacgctt?aggattgcag?agtttgctca?atttccaatg?gcacttagcg????1440

attggtgggc?aaactatttc?taaagctgaa?tttgacagac?tggtagcttt?aaaaagccca????1500

ttggtagaaa?ttaacggcga?gtgggtggaa?ttacgtcccc?aagatatcaa?aacagctgaa????1560

gcctttttta?ctgcgcgtaa?agaccaaatg?gccttatctt?tagaagatgc?cttacgtcta????1620

agtagtggcg?atacacaagt?aattgagaaa?ttaccagtag?tcagctttga?agcctctggc????1680

gcattacaag?aattgattgg?ggcgctgaca?aataatcaag?cagttgcacc?attacctacg????1740

ccgaaaaact?tccaaggaca?gttacgtcct?tatcaagaaa?ggggtgcggc?ttggttggcg????1800

ttcctcgaac?gctggggttt?aggtgcttgt?ctcgccgacg?acatgggact?gggaaaaacg????1860

atacagttca?ttgctttcct?tctccatctt?aaagaacagg?atgtattaga?aaaaccaact????1920

ttactagtgt?gtcctacttc?tgttttaggt?aactgggaac?gagaggtgag?aaaatttgca????1980

cctacactta?aagttctcca?gtatcatggt?gacaaacgtc?ctaaaggtaa?agcatttcaa????2040

gaagcagtaa?aaaaacatga?tttagttatt?acaagttact?cattaattca?tagagatatc????2100

aaatcattgc?agggtattcc?ttggcaaata?attgttttag?atgaagccca?aaatgtgaag????2160

aatgcggaag?ccaaacaatc?acaagcagtc?agacaattag?aaacaacatt?tcgtattgct????2220

ttaacaggta?caccagtaga?aaatagacta?caagaacttt?ggtcaatttt?agattttctt????2280

aatcctggtt?acttaggtaa?taagcaattc?tttcaaagac?gttttgctat?gccaattgaa????2340

aagtatggtg?atgcagcatc?tttaaatcaa?ttgcgtgctt?tagtgcaacc?atttattctg????2400

cgtcggctga?aaacagaccg?tgatattatt?caagacttgc?ccgataagca?agaaatgaca????2460

gtattttgtg?gtttgactgg?agaacaagct?gcactttatc?aaaaagcggt?agaaacatct????2520

ttagcagaaa?ttgaatcagc?cgaaggattg?caacgccgag?ggatgatttt?agctttatta????2580

attaaactca?aacaaatctg?caatcatcca?gcccaatatc?tgaaaataaa?tacattagaa????2640

caacacagtt?ctggaaaact?gcaaagatta?gaagaaatgt?tagaagaggt?gttagcagag??2700

agtaatactt?acggtgttgc?cggtgcggga?cgtgctttga?tttttaccca?atttgcagaa??2760

tggggtaagt?tactcaaacc?acatttagaa?aaacaactag?ggcgggaaat?atttttctta??2820

tatggtggta?cgagtaaaaa?gcaacgagaa?gaaatgattg?accgttttca?acacgacccc??2880

caagggccac?caattatgat?tctctccctc?aaagcaggtg?gtgtagggtt?gaacttaacc??2940

agggcaaatc?atgtatttca?ctttgataga?tggtggaatc?cagccgtaga?gaatcaagct??3000

acagaccgcg?tatttcgcat?tggtcaaact?cgcaatgtac?aggtgcataa?atttgtttgt??3060

aatggcacct?tagaagagaa?aattcacgac?atgattgaaa?gtaaaaaaca?actagcggaa??3120

caggttgttg?gagcaggcga?agaatggtta?actgaattag?atacagatca?actccgcaac??3180

ttactgatac?ttgatcgtag?tacagtaatt?gatgaagaag?cagattga???????????????3228

<210>60

<211>1075

<212>PRT

＜213〉beads algae

<400>60

Met?Ala?Ile?Leu?His?Gly?Ser?Trp?Ile?Leu?Asn?Glu?Gln?Glu?Ser?Cys

1???????????????5???????????????????10??????????????????15

Leu?Phe?Ile?Trp?Gly?Glu?Thr?Trp?Arg?Ser?Pro?Gln?Val?Asp?Phe?Asn

20??????????????????25??????????????????30

Phe?Ala?Glu?Ile?Ser?Leu?Asn?Pro?Leu?Ala?Leu?Ser?Ala?Leu?Glu?Leu

35??????????????????40??????????????????45

Ser?Glu?Trp?Leu?Gln?Ser?Gln?His?Gln?Ala?Ile?Ala?Lys?Leu?Leu?Pro

50??????????????????55??????????????????60

Gln?Gln?Leu?Glu?Lys?Arg?Thr?Ser?Lys?Ala?Ala?Ser?Ser?Val?Lys?Ile

65??????????????????70??????????????????75??????????????????80

Asn?Leu?Leu?Thr?His?Ser?Gln?Ile?Ile?Ala?Leu?Pro?Thr?Glu?Ile?Ser

85??????????????????90??????????????????95

Gln?Pro?Arg?Lys?Lys?Glu?Thr?Ile?Leu?Ile?Ser?Pro?Val?His?Ser?Ala

100?????????????????105?????????????????110

Ala?Leu?Ala?Ser?Glu?Ser?Asp?Ser?Glu?Val?Tyr?Leu?Gln?Thr?Trp?Arg

115?????????????????120?????????????????125

Val?Glu?Gly?Phe?Cys?Leu?Pro?Pro?Ser?Ala?Ala?Ile?Lys?Leu?Leu?Thr

130?????????????????135?????????????????140

Ser?Leu?Pro?Leu?Asn?Ile?Thr?Ser?Gly?Glu?Asn?Ala?Phe?Leu?Gly?Gly

145?????????????????150?????????????????155?????????????????160

Asp?Leu?Arg?Phe?Trp?Ser?Gln?Ile?Ala?Arg?Trp?Ser?Leu?Asp?Leu?Ile

165?????????????????170?????????????????175

Ser?Arg?Ser?Lys?Phe?Leu?Pro?Ile?Ile?Gln?Arg?Gln?Pro?Asn?Asn?Ser

180?????????????????185?????????????????190

Val?Ser?Ala?Lys?Trp?Gln?Val?Leu?Leu?Asp?Ser?Ala?Val?Asp?Gly?Thr

195?????????????????200?????????????????205

Arg?Leu?Glu?Lys?Phe?Ala?Ala?Lys?Met?Pro?Leu?Val?Cys?Arg?Thr?Tyr

210?????????????????215?????????????????220

Gln?Glu?Ile?Gly?Ser?Gly?Glu?Ser?Pro?Ile?Tyr?Ile?Asp?Phe?Pro?Ser

225?????????????????230?????????????????235?????????????????240

Gln?Pro?Gln?Asp?Leu?Ile?Leu?Gly?Phe?Leu?Asn?Ser?Ala?Ile?Asp?Thr

245?????????????????250?????????????????255

Gln?Leu?Arg?Glu?Met?Val?Gly?Asn?Gln?Pro?Val?Val?Glu?Thr?Arg?Leu

260?????????????????265?????????????????270

Met?Ala?Ser?Leu?Pro?Ser?Ala?Val?Arg?Gln?Trp?Leu?Gln?Ala?Leu?Ile

275?????????????????280?????????????????285

Ala?Ala?Ser?Asn?Ser?Ile?Asp?Ala?Asp?Ala?Val?Gly?Leu?Glu?Arg?Leu

290?????????????????295?????????????????300

Glu?Ala?Ala?Leu?Lys?Ala?Trp?Thr?Met?Pro?Leu?Gln?Tyr?Gln?Leu?Ala

305?????????????????310?????????????????315?????????????????320

Ser?Lys?Asn?Gln?Phe?Arg?Thr?Cys?Phe?Glu?Leu?Arg?Ser?Pro?Glu?Pro

325?????????????????330?????????????????335

Asp?Glu?Thr?Glu?Trp?Thr?Leu?Ala?Tyr?Phe?Leu?Gln?Ala?Ala?Asp?Asp

340?????????????????345?????????????????350

Pro?Glu?Phe?Leu?Val?Asp?Ala?Ala?Thr?Ile?Trp?Gln?Asn?Pro?Val?Glu

355?????????????????360?????????????????365

Gln?Leu?Ile?Tyr?Gln?Gln?Arg?Thr?Ile?Glu?Glu?Pro?Gln?Glu?Thr?Phe

370?????????????????375?????????????????380

Leu?Arg?Gly?Leu?Gly?Leu?Ala?Ser?Arg?Leu?Tyr?Pro?Val?Ile?Ala?Pro

385?????????????????390?????????????????395?????????????????400

Thr?Leu?Asp?Thr?Glu?Ser?Pro?Gln?Phe?Cys?His?Leu?Lys?Pro?Met?Gln

405?????????????????410?????????????????415

Ala?Tyr?Glu?Phe?Ile?Lys?Ala?Val?Ala?Trp?Arg?Phe?Glu?Asp?Ser?Gly

420?????????????????425?????????????????430

Leu?Gly?Val?Ile?Leu?Pro?Pro?Ser?Leu?Ala?Asn?Arg?Glu?Gly?Trp?Ala

435?????????????????440?????????????????445

Asn?Arg?Leu?Gly?Leu?Lys?Ile?Ser?Ala?Glu?Thr?Pro?Lys?Lys?Lys?Pro

450?????????????????455?????????????????460

Gly?Arg?Leu?Gly?Leu?Gln?Ser?Leu?Leu?Asn?Phe?Gln?Trp?His?Leu?Ala

465?????????????????470?????????????????475?????????????????480

Ile?Gly?Gly?Gln?Thr?Ile?Ser?Lys?Ala?Glu?Phe?Asp?Arg?Leu?Val?Ala

485?????????????????490?????????????????495

Leu?Lys?Ser?Pro?Leu?Val?Glu?Ile?Asn?Gly?Glu?Trp?Val?Glu?Leu?Arg

500?????????????????505?????????????????510

Pro?Gln?Asp?Ile?Lys?Thr?Ala?Glu?Ala?Phe?Phe?Thr?Ala?Arg?Lys?Asp

515?????????????????520?????????????????525

Gln?Met?Ala?Leu?Ser?Leu?Glu?Asp?Ala?Leu?Arg?Leu?Ser?Ser?Gly?Asp

530?????????????????535?????????????????540

Thr?Gln?Val?Ile?Glu?Lys?Leu?Pro?Val?Val?Ser?Phe?Glu?Ala?Ser?Gly

545?????????????????550?????????????????555?????????????????560

Ala?Leu?Gln?Glu?Leu?Ile?Gly?Ala?Leu?Thr?Asn?Asn?Gln?Ala?Val?Ala

565?????????????????570?????????????????575

Pro?Leu?Pro?Thr?Pro?Lys?Asn?Phe?Gln?Gly?Gln?Leu?Arg?Pro?Tyr?Gln

580?????????????????585?????????????????590

Glu?Arg?Gly?Ala?Ala?Trp?Leu?Ala?Phe?Leu?Glu?Arg?Trp?Gly?Leu?Gly

595?????????????????600?????????????????605

Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Phe?Ile

610?????????????????615?????????????????620

Ala?Phe?Leu?Leu?His?Leu?Lys?Glu?Gln?Asp?Val?Leu?Glu?Lys?Pro?Thr

625?????????????????630?????????????????635?????????????????640

Leu?Leu?Val?Cys?Pro?Thr?Ser?Val?Leu?Gly?Asn?Trp?Glu?Arg?Glu?Val

645?????????????????650?????????????????655

Arg?Lys?Phe?Ala?Pro?Thr?Leu?Lys?Val?Leu?Gln?Tyr?His?Gly?Asp?Lys

660?????????????????665?????????????????670

Arg?Pro?Lys?Gly?Lys?Ala?Phe?Gln?Glu?Ala?Val?Lys?Lys?His?Asp?Leu

675?????????????????680?????????????????685

Val?Ile?Thr?Ser?Tyr?Ser?Leu?Ile?His?Arg?Asp?Ile?Lys?Ser?Leu?Gln

690?????????????????695?????????????????700

Gly?Ile?Pro?Trp?Gln?Ile?Ile?Val?Leu?Asp?Glu?Ala?Gln?Asn?Val?Lys

705?????????????????710?????????????????715?????????????????720

Asn?Ala?Glu?Ala?Lys?Gln?Ser?Gln?Ala?Val?Arg?Gln?Leu?Glu?Thr?Thr

725?????????????????730?????????????????735

Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu?Gln?Glu

740?????????????????745?????????????????750

Leu?Trp?Ser?Ile?Leu?Asp?Phe?Leu?Asn?Pro?Gly?Tyr?Leu?Gly?Asn?Lys

755?????????????????760?????????????????765

Gln?Phe?Phe?Gln?Arg?Arg?Phe?Ala?Met?Pro?Ile?Glu?Lys?Tyr?Gly?Asp

770?????????????????775?????????????????780

Ala?Ala?Ser?Leu?Asn?Gln?Leu?Arg?Ala?Leu?Val?Gln?Pro?Phe?Ile?Leu

785?????????????????790?????????????????795?????????????????800

Arg?Arg?Leu?Lys?Thr?Asp?Arg?Asp?Ile?Ile?Gln?Asp?Leu?Pro?Asp?Lys

805?????????????????810?????????????????815

Gln?Glu?Met?Thr?Val?Phe?Cys?Gly?Leu?Thr?Gly?Glu?Gln?Ala?Ala?Leu

820?????????????????825?????????????????830

Tyr?Gln?Lys?Ala?Val?Glu?Thr?Ser?Leu?Ala?Glu?Ile?Glu?Ser?Ala?Glu

835?????????????????840?????????????????845

Gly?Leu?Gln?Arg?Arg?Gly?Met?Ile?Leu?Ala?Leu?Leu?Ile?Lys?Leu?Lys

850?????????????????855?????????????????860

Gln?Ile?Cys?Asn?His?Pro?Ala?Gln?Tyr?Leu?Lys?Ile?Asn?Thr?Leu?Glu

865?????????????????870?????????????????875?????????????????880

Gln?His?Ser?Ser?Gly?Lys?Leu?Gln?Arg?Leu?Glu?Glu?Met?Leu?Glu?Glu

885?????????????????890?????????????????895

Val?Leu?Ala?Glu?Ser?Asn?Thr?Tyr?Gly?Val?Ala?Gly?Ala?Gly?Arg?Ala

900?????????????????905?????????????????910

Leu?Ile?Phe?Thr?Gln?Phe?Ala?Glu?Trp?Gly?Lys?Leu?Leu?Lys?Pro?His

915?????????????????920?????????????????925

Leu?Glu?Lys?Gln?Leu?Gly?Arg?Glu?Ile?Phe?Phe?Leu?Tyr?Gly?Gly?Thr

930?????????????????935?????????????????940

Ser?Lys?Lys?Gln?Arg?Glu?Glu?Met?Ile?Asp?Arg?Phe?Gln?His?Asp?Pro

945?????????????????950?????????????????955?????????????????960

Gln?Gly?Pro?Pro?Ile?Met?Ile?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly

965?????????????????970?????????????????975

Leu?Asn?Leu?Thr?Arg?Ala?Asn?His?Val?Phe?His?Phe?Asp?Arg?Trp?Trp

980?????????????????985?????????????????990

Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala??Thr?Asp?Arg?Val?Phe??Arg?Ile?Gly

995?????????????????1000?????????????????1005

Gln?Thr??Arg?Asn?Val?Gln?Val??His?Lys?Phe?Val?Cys??Asn?Gly?Thr

1010?????????????????1015?????????????????1020

Leu?Glu??Glu?Lys?Ile?His?Asp??Met?Ile?Glu?Ser?Lys??Lys?Gln?Leu

1025?????????????????1030?????????????????1035

Ala?Glu??Gln?Val?Val?Gly?Ala??Gly?Glu?Glu?Trp?Leu??Thr?Glu?Leu

1040?????????????????1045?????????????????1050

Asp?Thr??Asp?Gln?Leu?Arg?Asn??Leu?Leu?Ile?Leu?Asp??Arg?Ser?Thr

1055?????????????????1060?????????????????1065

Val?Ile??Asp?Glu?Glu?Ala?Asp

1070?????????????????1075

<210>61

<211>3168

<212>DNA

＜213〉beads algae

<400>61

atgaaagtcc?ttcatggctc?gtggatacca?aaccaatata?gcgattttgt?gcagtctgga????60

gcattttatc?tatgggtaga?aactccgatt?aataacaaaa?agcgtactca?tacacaagtt????120

catcccggac?atctatcttc?tcttgaatta?ctcaattttc?tgactcaaac?tttggggatt????180

aaagaaactg?aagcgcaatt?aaaacaacgg?atatgttcta?aatattttgc?cctaccaact????240

gctaataatg?agccattacc?ttcaccagag?ttagtcaaat?atttagaagt?agaagttcct????300

gaagagtatg?aaaattttca?atattggcag?gtaacttgtt?atgaaactgt?tacttctgtg????360

aaagcagtga?tagcaattaa?tattattaaa?ttactcaaag?atattcattt?tttagccctg????420

tacaatgcta?gtgaatttca?attagggtca?gatttattat?tttggtatca?ttatacgcaa????480

tcatttagac?aaataattac?taaggatcaa?tatattccat?ctttaaaata?tagagcgaac????540

gcagcgacta?caaagaaaaa?acctaaacaa?ccacccccag?gatttgaaat?atatgctggt????600

tgggaaataa?tttccgagca?atacgaagcc?aatattcaaa?aatatattga?atatatgcca????660

ttgatttgtg?tagcaggtaa?cagcacacaa?actgataaat?tagaattttt?tgctccagaa????720

actctattac?gccacttcag?cgagtatctg?cttaataatt?tagtgagtaa?gacaccattg????780

accgcagcat?ttgaaaaaca?aattgatgat?tctttaattc?actattgtct?ttatccccaa????840

aaacacaacc?cactcaaaac?ccatactgct?ctccaagagt?atcagcagtg?gttgggatgg????900

aaaaacagga?ttatccgtac?tcaagctgaa?tcaccatttc?atctttgctt?ccaattacat????960

tcacctgatg?ctgaacaaat?tgacaattgg?cagatgcaat?ttttagtatc?aagtaaaaaa???1020

gatccgtctc?taaaattagc?tttggcagat?tactggataa?tgaattccaa?aaccaaagct???1080

ggtgtacata?aagagtttgg?caaagatttc?gatactaatt?tactgctgaa?tttaggctat???1140

gcagcaagaa?tgtatcccaa?actttggcaa?ggtttagaaa?cggactctcc?cacaggaatg???1200

cagctaagtt?tagatgaggc?gtttgatttt?ctcaaagata?gtgcttgggt?gttggaagac???1260

tcaggattta?aggtcattgt?cccggcttgg?tatactccgg?ctggtcgtcg?tcgtgcgaaa???1320

atccgcctca?aagcttctag?tggtcgcaag?gtagctgcta?cggtagggga?aagcaaaagt???1380

tatttcggtt?tagattcact?agtgcagtat?cagtatgaat?tagcaattgg?agagcaaact????1440

ctcacacctc?aagaatggga?acaattgatt?aatactaaag?caccactagt?gcattttcgc????1500

ggtcaatgga?tggaattaga?ccgggataaa?atgcagcagt?tattagaatt?ttggcagtcc????1560

cacggcgatg?aacagcccca?aatgagcttg?ttagagttca?tgcaacgcag?cgcccaaggg????1620

gaagatgact?gggaaattga?atatgatgca?gctttatcag?aaataatggc?aaagttacaa????1680

gataagagtc?agctagagcc?aatttctgaa?gacttaaatt?tgcaaggcaa?cctgcgagaa????1740

tatcaaaagc?ggggtgtagc?ctggttacaa?tatttagaaa?aattgggatt?aaatggctgt????1800

ttagccgatg?atatgggact?gggtaagtcc?gtgcaggtaa?ttgcgagatt?agtacaggag????1860

aaagatagcc?aaagttcccc?attaccgaca?ttattaattg?cgccgacttc?ggttgttggt????1920

aactggcaaa?gagaaattgc?taagtttgca?ccccatttaa?aaactatggt?gcatcatggt????1980

agcgatcgcc?tgcaagatgc?tgcggagttt?aagtccgcct?gtcaacagca?tgatgtggtg????2040

ataagttcct?ttactttggc?tcgcttagat?gaaaaactcc?taaatagtgt?gacatggcaa????2100

cggttagttt?tagatgaagc?acaaaacatt?aaaaatccca?aagcagcgca?gactaaagct????2160

atactcaaac?tcagtgctaa?acaccgtcta?gctttaactg?gtacaccagt?tgagaaccgc????2220

ttacttgatt?tgtggtcaat?ttttaatttt?ctcaatcccg?gttatttagg?gaaagaagca????2280

cagtttcgca?aatcctttga?aattcccatc?cagaaggaca?acgataaagt?aaaatcgact????2340

accttaaaga?aactggttga?accgttaatt?ttacgacggg?tcaaaacaga?ccaatcaatt????2400

attaaagact?taccagataa?agttgaacaa?aaactctata?ccaacctcac?caaagaacag????2460

gcttcgctat?atgaagtggt?agtcagagat?gtggaagaaa?aattgcaaga?agctgaggga????2520

atacaacgca?aaggtttaat?tctctcaacg?ctgatgaaat?taaaacagat?ttgcaatcat????2580

cccagacagt?tcctccaaga?taatagcgaa?tttttaccgg?agcgctcgca?caaactttcc????2640

cgcttagtcg?aaatggtaga?tgaagccatt?tctgaaggag?aaagtctttt?aatatttagt????2700

caatttacag?aagtctgcga?acaaatagaa?aaatatctca?aacacaactt?acattgcaat??2760

acctactacc?tacatggggg?tacaagtcgc?caacgtcggg?aacaaatgat?tagtgacttt??2820

caaaatcctg?atacggaagc?atctgtattt?gtcctttccc?taaaagctgg?cggcgtgggg??2880

attactttaa?ctaaagccaa?ccacgtcttt?cattttgacc?gttggtggaa?tccagccgtt??2940

gaagaccaag?ccacagaccg?cgcttttcgc?ataggtcaga?aaaaaaatgt?gtttgtacat??3000

aaatttgtcg?cccttgggac?tttagaagaa?agaatcgacc?aaatgattga?agataagaaa??3060

aaactttctt?ccgccgtagt?tggtagtgat?gaatcgtggc?taaccgaatt?agataacgaa??3120

gcctttaaga?aactaattgc?cttgaataaa?agcacaatta?tggagtag???????????????3168

<210>62

<211>1055

<212>PRT

＜213〉beads algae

<400>62

Met?Lys?Val?Leu?His?Gly?Ser?Trp?Ile?Pro?Asn?Gln?Tyr?Ser?Asp?Phe

1???????????????5???????????????????10??????????????????15

Val?Gln?Ser?Gly?Ala?Phe?Tyr?Leu?Trp?Val?Glu?Thr?Pro?Ile?Asn?Asn

20??????????????????25??????????????????30

Lys?Lys?Arg?Thr?His?Thr?Gln?Val?His?Pro?Gly?His?Leu?Ser?Ser?Leu

35??????????????????40??????????????????45

Glu?Leu?Leu?Asn?Phe?Leu?Thr?Gln?Thr?Leu?Gly?Ile?Lys?Glu?Thr?Glu

50??????????????????55??????????????????60

Ala?Gln?Leu?Lys?Gln?Arg?Ile?Cys?Ser?Lys?Tyr?Phe?Ala?Leu?Pro?Thr

65??????????????????70??????????????????75??????????????????80

Ala?Asn?Asn?Glu?Pro?Leu?Pro?Ser?Pro?Glu?Leu?Val?Lys?Tyr?Leu?Glu

85??????????????????90??????????????????95

Val?Glu?Val?Pro?Glu?Glu?Tyr?Glu?Asn?Phe?Gln?Tyr?Trp?Gln?Val?Thr

100?????????????????105?????????????????110

Cys?Tyr?Glu?Thr?Val?Thr?Ser?Val?Lys?Ala?Val?Ile?Ala?Ile?Asn?Ile

115?????????????????120?????????????????125

Ile?Lys?Leu?Leu?Lys?Asp?Ile?His?Phe?Leu?Ala?Leu?Tyr?Asn?Ala?Ser

130?????????????????135?????????????????140

Glu?Phe?Gln?Leu?Gly?Ser?Asp?Leu?Leu?Phe?Trp?Tyr?His?Tyr?Thr?Gln

145?????????????????150?????????????????155?????????????????160

Ser?Phe?Arg?Gln?Ile?Ile?Thr?Lys?Asp?Gln?Tyr?Ile?Pro?Ser?Leu?Lys

165?????????????????170?????????????????175

Tyr?Arg?Ala?Asn?Ala?Ala?Thr?Thr?Lys?Lys?Lys?Pro?Lys?Gln?Pro?Pro

180?????????????????185?????????????????190

Pro?Gly?Phe?Glu?Ile?Tyr?Ala?Gly?Trp?Glu?Ile?Ile?Ser?Glu?Gln?Tyr

195?????????????????200?????????????????205

Glu?Ala?Asn?Ile?Gln?Lys?Tyr?Ile?Glu?Tyr?Met?Pro?Leu?Ile?Cys?Val

210?????????????????215?????????????????220

Ala?Gly?Asn?Ser?Thr?Gln?Thr?Asp?Lys?Leu?Glu?Phe?Phe?Ala?Pro?Glu

225?????????????????230?????????????????235?????????????????240

Thr?Leu?Leu?Arg?His?Phe?Ser?Glu?Tyr?Leu?Leu?Asn?Asn?Leu?Val?Ser

245?????????????????250?????????????????255

Lys?Thr?Pro?Leu?Thr?Ala?Ala?Phe?Glu?Lys?Gln?Ile?Asp?Asp?Ser?Leu

260?????????????????265?????????????????270

Ile?His?Tyr?Cys?Leu?Tyr?Pro?Gln?Lys?His?Asn?Pro?Leu?Lys?Thr?His

275?????????????????280?????????????????285

Thr?Ala?Leu?Gln?Glu?Tyr?Gln?Gln?Trp?Leu?Gly?Trp?Lys?Asn?Arg?Ile

290?????????????????295?????????????????300

Ile?Arg?Thr?Gln?Ala?Glu?Ser?Pro?Phe?His?Leu?Cys?Phe?Gln?Leu?His

305?????????????????310?????????????????315?????????????????320

Ser?Pro?Asp?Ala?Glu?Gln?Ile?Asp?Asn?Trp?Gln?Met?Gln?Phe?Leu?Val

325?????????????????330?????????????????335

Ser?Ser?Lys?Lys?Asp?Pro?Ser?Leu?Lys?Leu?Ala?Leu?Ala?Asp?Tyr?Trp

340?????????????????345?????????????????350

Ile?Met?Asn?Ser?Lys?Thr?Lys?Ala?Gly?Val?His?Lys?Glu?Phe?Gly?Lys

355?????????????????360?????????????????365

Asp?Phe?Asp?Thr?Asn?Leu?Leu?Leu?Asn?Leu?Gly?Tyr?Ala?Ala?Arg?Met

370?????????????????375?????????????????380

Tyr?Pro?Lys?Leu?Trp?Gln?Gly?Leu?Glu?Thr?Asp?Ser?Pro?Thr?Gly?Met

385?????????????????390?????????????????395?????????????????400

Gln?Leu?Ser?Leu?Asp?Glu?Ala?Phe?Asp?Phe?Leu?Lys?Asp?Ser?Ala?Trp

405?????????????????410?????????????????415

Val?Leu?Glu?Asp?Ser?Gly?Phe?Lys?Val?Ile?Val?Pro?Ala?Trp?Tyr?Thr

420?????????????????425?????????????????430

Pro?Ala?Gly?Arg?Arg?Arg?Ala?Lys?Ile?Arg?Leu?Lys?Ala?Ser?Ser?Gly

435?????????????????440?????????????????445

Arg?Lys?Val?Ala?Ala?Thr?Val?Gly?Glu?Ser?Lys?Ser?Tyr?Phe?Gly?Leu

450?????????????????455?????????????????460

Asp?Ser?Leu?Val?Gln?Tyr?Gln?Tyr?Glu?Leu?Ala?Ile?Gly?Glu?Gln?Thr

465?????????????????470?????????????????475?????????????????480

Leu?Thr?Pro?Gln?Glu?Trp?Glu?Gln?Leu?Ile?Asn?Thr?Lys?Ala?Pro?Leu

485?????????????????490?????????????????495

Val?His?Phe?Arg?Gly?Gln?Trp?Met?Glu?Leu?Asp?Arg?Asp?Lys?Met?Gln

500?????????????????505?????????????????510

Gln?Leu?Leu?Glu?Phe?Trp?Gln?Ser?His?Gly?Asp?Glu?Gln?Pro?Gln?Met

515?????????????????520?????????????????525

Ser?Leu?Leu?Glu?Phe?Met?Gln?Arg?Ser?Ala?Gln?Gly?Glu?Asp?Asp?Trp

530?????????????????535?????????????????540

Glu?Ile?Glu?Tyr?Asp?Ala?Ala?Leu?Ser?Glu?Ile?Met?Ala?Lys?Leu?Gln

545?????????????????550?????????????????555?????????????????560

Asp?Lys?Ser?Gln?Leu?Glu?Pro?Ile?Ser?Glu?Asp?Leu?Asn?Leu?Gln?Gly

565?????????????????570?????????????????575

Asn?Leu?Arg?Glu?Tyr?Gln?Lys?Arg?Gly?Val?Ala?Trp?Leu?Gln?Tyr?Leu

580?????????????????585?????????????????590

Glu?Lys?Leu?Gly?Leu?Asn?Gly?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly

595?????????????????600?????????????????605

Lys?Ser?Val?Gln?Val?Ile?Ala?Arg?Leu?Val?Gln?Glu?Lys?Asp?Ser?Gln

610?????????????????615?????????????????620

Ser?Ser?Pro?Leu?Pro?Thr?Leu?Leu?Ile?Ala?Pro?Thr?Ser?Val?Val?Gly

625?????????????????630?????????????????635?????????????????640

Asn?Trp?Gln?Arg?Glu?Ile?Ala?Lys?Phe?Ala?Pro?His?Leu?Lys?Thr?Met

645?????????????????650?????????????????655

Val?His?His?Gly?Ser?Asp?Arg?Leu?Gln?Asp?Ala?Ala?Glu?Phe?Lys?Ser

660?????????????????665?????????????????670

Ala?Cys?Gln?Gln?His?Asp?Val?Val?Ile?Ser?Ser?Phe?Thr?Leu?Ala?Arg

675?????????????????680?????????????????685

Leu?Asp?Glu?Lys?Leu?Leu?Asn?Ser?Val?Thr?Trp?Gln?Arg?Leu?Val?Leu

690?????????????????695?????????????????700

Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn?Pro?Lys?Ala?Ala?Gln?Thr?Lys?Ala

705?????????????????710?????????????????715?????????????????720

Ile?Leu?Lys?Leu?Ser?Ala?Lys?His?Arg?Leu?Ala?Leu?Thr?Gly?Thr?Pro

725?????????????????730?????????????????735

Val?Glu?Asn?Arg?Leu?Leu?Asp?Leu?Trp?Ser?Ile?Phe?Asn?Phe?Leu?Asn

740?????????????????745?????????????????750

Pro?Gly?Tyr?Leu?Gly?Lys?Glu?Ala?Gln?Phe?Arg?Lys?Ser?Phe?Glu?Ile

755?????????????????760?????????????????765

Pro?Ile?Gln?Lys?Asp?Asn?Asp?Lys?Val?Lys?Ser?Thr?Thr?Leu?Lys?Lys

770?????????????????775?????????????????780

Leu?Val?Glu?Pro?Leu?Ile?Leu?Arg?Arg?Val?Lys?Thr?Asp?Gln?Ser?Ile

785?????????????????790?????????????????795?????????????????800

Ile?Lys?Asp?Leu?Pro?Asp?Lys?Val?Glu?Gln?Lys?Leu?Tyr?Thr?Asn?Leu

805?????????????????810?????????????????815

Thr?Lys?Glu?Gln?Ala?Ser?Leu?Tyr?Glu?Val?Val?Val?Arg?Asp?Val?Glu

820?????????????????825?????????????????830

Glu?Lys?Leu?Gln?Glu?Ala?Glu?Gly?Ile?Gln?Arg?Lys?Gly?Leu?Ile?Leu

835?????????????????840?????????????????845

Ser?Thr?Leu?Met?Lys?Leu?Lys?Gln?Ile?Cys?Asn?His?Pro?Arg?Gln?Phe

850?????????????????855?????????????????860

Leu?Gln?Asp?Asn?Ser?Glu?Phe?Leu?Pro?Glu?Arg?Ser?His?Lys?Leu?Ser

865?????????????????870?????????????????875?????????????????880

Arg?Leu?Val?Glu?Met?Val?Asp?Glu?Ala?Ile?Ser?Glu?Gly?Glu?Ser?Leu

885?????????????????890?????????????????895

Leu?Ile?Phe?Ser?Gln?Phe?Thr?Glu?Val?Cys?Glu?Gln?Ile?Glu?Lys?Tyr

900?????????????????905?????????????????910

Leu?Lys?His?Asn?Leu?His?Cys?Asn?Thr?Tyr?Tyr?Leu?His?Gly?Gly?Thr

915?????????????????920?????????????????925

Ser?Arg?Gln?Arg?Arg?Glu?Gln?Met?Ile?Ser?Asp?Phe?Gln?Asn?Pro?Asp

930?????????????????935?????????????????940

Thr?Glu?Ala?Ser?Val?Phe?Val?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly

945?????????????????950?????????????????955?????????????????960

Ile?Thr?Leu?Thr?Lys?Ala?Asn?His?Val?Phe?His?Phe?Asp?Arg?Trp?Trp

965?????????????????970?????????????????975

Asn?Pro?Ala?Val?Glu?Asp?Gln?Ala?Thr?Asp?Arg?Ala?Phe?Arg?Ile?Gly

980?????????????????985?????????????????990

Gln?Lys?Lys?Asn?Val?Phe?Val?His??Lys?Phe?Val?Ala?Leu??Gly?Thr?Leu

995?????????????????1000?????????????????1005

Glu?Glu??Arg?Ile?Asp?Gln?Met??Ile?Glu?Asp?Lys?Lys??Lys?Leu?Ser

1010?????????????????1015?????????????????1020

Ser?Ala??Val?Val?Gly?Ser?Asp??Glu?Ser?Trp?Leu?Thr??Glu?Leu?Asp

1025?????????????????1030?????????????????1035

Asn?Glu??Ala?Phe?Lys?Lys?Leu??Ile?Ala?Leu?Asn?Lys??Ser?Thr?Ile

1040?????????????????1045?????????????????1050

Met?Glu

1055

<210>63

<211>2856

<212>DNA

＜213〉some shape beads algae

<400>63

atggcgattt?tacacagtaa?ttggttacta?aaaagtcaaa?aaggttgttt?atttatttgg?????60

ggagaaactt?ggcgatcgcc?acgagttaat?ttcgagtcta?atggatctgg?agatatccca????120

ctaaatccat?tggcaatgac?atcactagag?ttgagcgagt?ggttggtttc?ccagaagatg????180

gccattacca?actttatcca?gcaaccccaa?attgccatcg?ctactactgg?gcgaacacgt????240

aaagcagcca?ctgccactga?gataaactta?ccaacgcatt?cacaaataat?tgccttacca????300

acttatattc?ccgaagagag?tgcagaagga?acatctgcaa?ttttccctgt?gcattctgcc????360

agcttgagac?tagaaacaga?ctctccgcaa?tatttgcaac?cgtggctagt?tgagggtttt????420

tgtcttaacc?ccagcgaagc?agtaaaattt?ctcgctgctg?ttcccctgaa?tgctgctaaa????480

ggggaagatg?cttttttagg?aggagattta?cgtttttggt?cgcaagtttc?ccgatggagt????540

ttagatttaa?tctcgcggtg?taagttttta?ccaagaattg?aacggcaatc?agacggtgca????600

tttgctgcta?aatggcaagt?acttctagac?agtgctgtag?atggaactcg?cctagaaaag????660

ttttctgcgg?atatgccgtt?ggtttgccgc?acttatcagg?agggagtggg?gactggggac????720

tggggactga?ggactgggga?ggagttttcc?caatccctaa?tccctaattc?ccaatcccta????780

ctttatgtaa?acttccctac?tgaacctcaa?gaattgttgc?tgggatttct?caacagtacg????840

atagatgccc?aagtgcgagg?gatggtgggt?tctcagcctc?caatggaagc?taaggcaatg????900

gcatctttac?catctggggt?gcggcagtgg?ttgcaaggct?tgactagtac?atctggtaca????960

gttaacgcag?atgccattga?agtggaacga?ctggaagcgg?cactgaaggc?ttggatgatg???1020

ccgctacaat?accaattaac?tcttaaaact?ctatttcgta?cctgttttca?actgcgttct???1080

ccagaagctg?gcgaaacaga?ttggacattg?gcgtattttc?tgcaagcggc?tgacgatcct???1140

gattttttgg?tggatgcggc?aactatttgg?aacaatccag?ttgaacgttt?ggtttatgaa???1200

aatcgaacaa?ttgagcaacc?acaggaaaca?tttttgcgag?gtttaggggt?agcttcccga???1260

ttatatccag?cgatcgcacc?cagttttgaa?accgaatatc?cccaatcttc?tcggatcaca???1320

cccatgcaag?cttatgagtt?tatcaaggct?gtagcttgga?ggttggaaga?cagtggtttg???1380

ggggtaattt?tgcctcctag?tttagcgaac?cgcgaaggat?gggcaaatcg?tttgggtttg???1440

aaaattactg?ctgaaacccc?aaagaaaaag?cagggacgtt?tagggttgca?aagtctgctg???1500

aatttccaat?ggcaattggc?aattggcgga?cagactattt?ccaaagctga?gtttgataaa???1560

cttgtggctt?taaatagtcc?actagtggaa?attaacggtg?agtgggtaga?attgcggccc???1620

caagatatca?agacagccca?aacatttttt?accactcgca?aagaccaaat?ggcgctttcc????1680

ttggaagatg?ccttgcgttt?cagtacagga?gatacccagg?taattgaaaa?attaccagtg????1740

gtcagctttg?aggcatctgg?ggcattgcaa?gagttgattg?gggcgctaaa?taataatcaa????1800

gcgatcgcac?ctttaccgac?accagtaggc?tttaaaggac?agttgcgacc?ttatcaagaa????1860

cgtggtgctg?cttggctgtc?cttcttggaa?cgttggggct?taggcgcgtg?tctcgccgac????1920

gatatgggac?tcggtaaaac?tattcagttt?attgcttttt?tgctacatct?taaagaacag????1980

gatgcactag?aaaattcaac?actgctagtt?tgtccaactt?ctgttttagg?caactgggaa????2040

agggaagtca?ataaatttgc?accaagcctg?aaaattttgc?aatatcacgg?tgacaaacgt????2100

ccaaaaggga?aagcgttttt?agaagcagtg?aaaaatcacg?atttaatcgt?taccagctac????2160

tcactgcttc?atcgggatat?caagtcattg?caaagtgttc?cttggcagat?aattgtttta????2220

gacgaagccc?agaatgtgaa?aaatccagag?gcgaagcagt?caaaagctgt?gcggcaatta????2280

gaagctacat?ttcgcattgc?attaacgggg?acaccagtag?aaaatagact?gcaagaacta????2340

tggtctattt?tggattttct?caatccaggg?tatttaggta?ataagcaatt?tttccagcgg????2400

cggtttgcca?tgccaattga?aaagtatggt?gatacggctt?ctttgggtca?attacgttca????2460

ttagttcagc?catttatact?gcggcgatta?aaaagcgatc?gcgaaattat?tcaagacttg????2520

ccagataagc?aagagatgac?cgtattttgc?ggtttaactg?ccgaccaagc?tgcactttat????2580

caacaagttg?tagaacaatc?tttagtagag?atagaatctg?ctgaaggatt?gcaacgtcgg????2640

gggatgattt?tggctttgct?aatcaaactg?aagcaaatct?gcaatcatcc?agcccaatat????2700

ttgaaacagg?cgacattaga?gcaacataat?tcagccaaac?ttctgcggct?agaagaaatg????2760

ttagaagaag?ttttagcaga?aagtgaccgg?gctttaatct?ttacacaatt?tgcagagtgg????2820

ggtaagttac?ttaaacccaa?aagtgttgaa?tgttaa??????????????????????????????2856

<210>64

<211>951

<212>PRT

＜213〉some shape beads algae

<400>64

Met?Ala?Ile?Leu?His?Ser?Asn?Trp?Leu?Leu?Lys?Ser?Gln?Lys?Gly?Cys

1???????????????5???????????????????10??????????????????15

Leu?Phe?Ile?Trp?Gly?Glu?Thr?Trp?Arg?Ser?Pro?Arg?Val?Asn?Phe?Glu

20??????????????????25??????????????????30

Ser?Asn?Gly?Ser?Gly?Asp?Ile?Pro?Leu?Asn?Pro?Leu?Ala?Met?Thr?Ser

35??????????????????40??????????????????45

Leu?Glu?Leu?Ser?Glu?Trp?Leu?Val?Ser?Gln?Lys?Met?Ala?Ile?Thr?Asn

50??????????????????55??????????????????60

Phe?Ile?Gln?Gln?Pro?Gln?Ile?Ala?Ile?Ala?Thr?Thr?Gly?Arg?Thr?Arg

65??????????????????70??????????????????75??????????????????80

Lys?Ala?Ala?Thr?Ala?Thr?Glu?Ile?Asn?Leu?Pro?Thr?His?Ser?Gln?Ile

85??????????????????90??????????????????95

Ile?Ala?Leu?Pro?Thr?Tyr?Ile?Pro?Glu?Glu?Ser?Ala?Glu?Gly?Thr?Ser

100?????????????????105?????????????????110

Ala?Ile?Phe?Pro?Val?His?Ser?Ala?Ser?Leu?Arg?Leu?Glu?Thr?Asp?Ser

115?????????????????120?????????????????125

Pro?Gln?Tyr?Leu?Gln?Pro?Trp?Leu?Val?Glu?Gly?Phe?Cys?Leu?Asn?Pro

130?????????????????135?????????????????140

Ser?Glu?Ala?Val?Lys?Phe?Leu?Ala?Ala?Val?Pro?Leu?Asn?Ala?Ala?Lys

145?????????????????150?????????????????155?????????????????160

Gly?Glu?Asp?Ala?Phe?Leu?Gly?Gly?Asp?Leu?Arg?Phe?Trp?Ser?Gln?Val

165?????????????????170?????????????????175

Ser?Arg?Trp?Ser?Leu?Asp?Leu?Ile?Ser?Arg?Cys?Lys?Phe?Leu?Pro?Arg

180?????????????????185?????????????????190

Ile?Glu?Arg?Gln?Ser?Asp?Gly?Ala?Phe?Ala?Ala?Lys?Trp?Gln?Val?Leu

195?????????????????200?????????????????205

Leu?Asp?Ser?Ala?Val?Asp?Gly?Thr?Arg?Leu?Glu?Lys?Phe?Ser?Ala?Asp

210?????????????????215?????????????????220

Met?Pro?Leu?Val?Cys?Arg?Thr?Tyr?Gln?Glu?Gly?Val?Gly?Thr?Gly?Asp

225?????????????????230?????????????????235?????????????????240

Trp?Gly?Leu?Arg?Thr?Gly?Glu?Glu?Phe?Ser?Gln?Ser?Leu?Ile?Pro?Asn

245?????????????????250?????????????????255

Ser?Gln?Ser?Leu?Leu?Tyr?Val?Asn?Phe?Pro?Thr?Glu?Pro?Gln?Glu?Leu

260?????????????????265?????????????????270

Leu?Leu?Gly?Phe?Leu?Asn?Ser?Thr?Ile?Asp?Ala?Gln?Val?Arg?Gly?Met

275?????????????????280?????????????????285

Val?Gly?Ser?Gln?Pro?Pro?Met?Glu?Ala?Lys?Ala?Met?Ala?Ser?Leu?Pro

290?????????????????295?????????????????300

Ser?Gly?Val?Arg?Gln?Trp?Leu?Gln?Gly?Leu?Thr?Ser?Thr?Ser?Gly?Thr

305?????????????????310?????????????????315?????????????????320

Val?Asn?Ala?Asp?Ala?Ile?Glu?Val?Glu?Arg?Leu?Glu?Ala?Ala?Leu?Lys

325?????????????????330?????????????????335

Ala?Trp?Met?Met?Pro?Leu?Gln?Tyr?Gln?Leu?Thr?Leu?Lys?Thr?Leu?Phe

340?????????????????345?????????????????350

Arg?Thr?Cys?Phe?Gln?Leu?Arg?Ser?Pro?Glu?Ala?Gly?Glu?Thr?Asp?Trp

355?????????????????360?????????????????365

Thr?Leu?Ala?Tyr?Phe?Leu?Gln?Ala?Ala?Asp?Asp?Pro?Asp?Phe?Leu?Val

370?????????????????375?????????????????380

Asp?Ala?Ala?Thr?Ile?Trp?Asn?Asn?Pro?Val?Glu?Arg?Leu?Val?Tyr?Glu

385?????????????????390?????????????????395?????????????????400

Asn?Arg?Thr?Ile?Glu?Gln?Pro?Gln?Glu?Thr?Phe?Leu?Arg?Gly?Leu?Gly

405?????????????????410?????????????????415

Val?Ala?Ser?Arg?Leu?Tyr?Pro?Ala?Ile?Ala?Pro?Ser?Phe?Glu?Thr?Glu

420?????????????????425?????????????????430

Tyr?Pro?Gln?Ser?Ser?Arg?Ile?Thr?Pro?Met?Gln?Ala?Tyr?Glu?Phe?Ile

435?????????????????440?????????????????445

Lys?Ala?Val?Ala?Trp?Arg?Leu?Glu?Asp?Ser?Gly?Leu?Gly?Val?Ile?Leu

450?????????????????455?????????????????460

Pro?Pro?Ser?Leu?Ala?Asn?Arg?Glu?Gly?Trp?Ala?Asn?Arg?Leu?Gly?Leu

465?????????????????470?????????????????475?????????????????480

Lys?Ile?Thr?Ala?Glu?Thr?Pro?Lys?Lys?Lys?Gln?Gly?Arg?Leu?Gly?Leu

485?????????????????490?????????????????495

Gln?Ser?Leu?Leu?Asn?Phe?Gln?Trp?Gln?Leu?Ala?Ile?Gly?Gly?Gln?Thr

500?????????????????505?????????????????510

Ile?Ser?Lys?Ala?Glu?Phe?Asp?Lys?Leu?Val?Ala?Leu?Asn?Ser?Pro?Leu

515?????????????????520?????????????????525

Val?Glu?Ile?Asn?Gly?Glu?Trp?Val?Glu?Leu?Arg?Pro?Gln?Asp?Ile?Lys

530?????????????????535?????????????????540

Thr?Ala?Gln?Thr?Phe?Phe?Thr?Thr?Arg?Lys?Asp?Gln?Met?Ala?Leu?Ser

545?????????????????550?????????????????555?????????????????560

Leu?Glu?Asp?Ala?Leu?Arg?Phe?Ser?Thr?Gly?Asp?Thr?Gln?Val?Ile?Glu

565?????????????????570?????????????????575

Lys?Leu?Pro?Val?Val?Ser?Phe?Glu?Ala?Ser?Gly?Ala?Leu?Gln?Glu?Leu

580?????????????????585?????????????????590

Ile?Gly?Ala?Leu?Asn?Asn?Asn?Gln?Ala?Ile?Ala?Pro?Leu?Pro?Thr?Pro

595?????????????????600?????????????????605

Val?Gly?Phe?Lys?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Ala?Ala

610?????????????????615?????????????????620

Trp?Leu?Ser?Phe?Leu?Glu?Arg?Trp?Gly?Leu?Gly?Ala?Cys?Leu?Ala?Asp

625?????????????????630?????????????????635?????????????????640

Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Phe?Ile?Ala?Phe?Leu?Leu?His

645?????????????????650?????????????????655

Leu?Lys?Glu?Gln?Asp?Ala?Leu?Glu?Asn?Ser?Thr?Leu?Leu?Val?Cys?Pro

660?????????????????665?????????????????670

Thr?Ser?Val?Leu?Gly?Asn?Trp?Glu?Arg?Glu?Val?Asn?Lys?Phe?Ala?Pro

675?????????????????680?????????????????685

Ser?Leu?Lys?Ile?Leu?Gln?Tyr?His?Gly?Asp?Lys?Arg?Pro?Lys?Gly?Lys

690?????????????????695?????????????????700

Ala?Phe?Leu?Glu?Ala?Val?Lys?Asn?His?Asp?Leu?Ile?Val?Thr?Ser?Tyr

705?????????????????710?????????????????715?????????????????720

Ser?Leu?Leu?His?Arg?Asp?Ile?Lys?Ser?Leu?Gln?Ser?Val?Pro?Trp?Gln

725?????????????????730?????????????????735

Ile?Ile?Val?Leu?Asp?Glu?Ala?Gln?Asn?Val?Lys?Asn?Pro?Glu?Ala?Lys

740?????????????????745?????????????????750

Gln?Ser?Lys?Ala?Val?Arg?Gln?Leu?Glu?Ala?Thr?Phe?Arg?Ile?Ala?Leu

755?????????????????760?????????????????765

Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu?Gln?Glu?Leu?Trp?Ser?Ile?Leu

770?????????????????775?????????????????780

Asp?Phe?Leu?Asn?Pro?Gly?Tyr?Leu?Gly?Asn?Lys?Gln?Phe?Phe?Gln?Arg

785?????????????????790?????????????????795?????????????????800

Arg?Phe?Ala?Met?Pro?Ile?Glu?Lys?Tyr?Gly?Asp?Thr?Ala?Ser?Leu?Gly

805?????????????????810?????????????????815

Gln?Leu?Arg?Ser?Leu?Val?Gln?Pro?Phe?Ile?Leu?Arg?Arg?Leu?Lys?Ser

820?????????????????825?????????????????830

Asp?Arg?Glu?Ile?Ile?Gln?Asp?Leu?Pro?Asp?Lys?Gln?Glu?Met?Thr?Val

835?????????????????840?????????????????845

Phe?Cys?Gly?Leu?Thr?Ala?Asp?Gln?Ala?Ala?Leu?Tyr?Gln?Gln?Val?Val

850?????????????????855?????????????????860

Glu?Gln?Ser?Leu?Val?Glu?Ile?Glu?Ser?Ala?Glu?Gly?Leu?Gln?Arg?Arg

865?????????????????870?????????????????875?????????????????880

Gly?Met?Ile?Leu?Ala?Leu?Leu?Ile?Lys?Leu?Lys?Gln?Ile?Cys?Asn?His

885?????????????????890?????????????????895

Pro?Ala?Gln?Tyr?Leu?Lys?Gln?Ala?Thr?Leu?Glu?Gln?His?Asn?Ser?Ala

900?????????????????905?????????????????910

Lys?Leu?Leu?Arg?Leu?Glu?Glu?Met?Leu?Glu?Glu?Val?Leu?Ala?Glu?Ser

915?????????????????920?????????????????925

Asp?Arg?Ala?Leu?Ile?Phe?Thr?Gln?Phe?Ala?Glu?Trp?Gly?Lys?Leu?Leu

930?????????????????935?????????????????940

Lys?Pro?Lys?Ser?Val?Glu?Cys

945?????????????????950

<210>65

<211>3024

<212>DNA

<213>Pelodictyon?phaeoclathratiforme

<400>65

atgattgcgc?tgcacatctc?catcattgac?ggagtcccgc?tactctggag?tgagggaaaa?????60

aagatcggga?tgctgaagga?gttacgcctc?gcaacggctg?gaatcggcat?gttttccctg????120

ctcgacaaca?ccacaaaaga?gttttgtgtc?tggctgccct?gccgcgagaa?aaaagctgtc????180

ccatcatctc?cgcttgtcgg?cgccatgccc?gacctgagtg?atgaagagca?actccatgcc????240

tttccgatta?ccgcgcttcg?gctgaatttc?aacgctctgt?tcgagctttc?cctgcttacg????300

gaaaagggca?acatccccgg?cagtggcatc?atcttcggaa?gctctctcca?ctgggcacgg????360

caggtagtaa?aaattgcact?gaacattgtc?agaacccagt?cgctgctccc?ttcgatcatc?????420

aaaaacgata?cattctggga?ggccttgtgg?ttgcccctcc?ccgacagtgc?cacatccctc?????480

gcagttgaac?agcttgccga?tgccatgcct?gcggtctgtc?gctctctcgg?ccgcaccgac?????540

acgcaaccgc?cggaaacacc?aaaaaagtta?ctgctcaaag?gacttctctc?tttccttgtc?????600

aatacactgt?cacgtacttt?tgaaagagca?ggggtgccaa?aaatcagtga?cttcgagagt?????660

atccatgacg?cgtggcttca?tgcattatca?aacagtgatc?cccggctgaa?atggaaaaat?????720

gagcaggaga?ttgagcagtt?tgcctgtcag?ctcaacgcat?ggcggcgtcc?cattgacctg?????780

catgagcgat?cacccttcag?gttttgcctg?caactgacag?agccaccact?gaaagggcgg?????840

aaaaaggagc?gctggcatgt?tgcctatcaa?ctgcagttga?aagcggatcc?aagcctgatt?????900

cttgacgccg?gggatctctg?gaaccccgaa?agcgaggcat?cacagcacgc?tttaacgtat?????960

acctccgatt?gtaccgaatt?cctgcttact?tccctgggac?aagcctccgg?cctctgcccc????1020

gcagtcaccc?aaagcctgaa?aaagaagcag?ccgggtggct?ttgatcttga?taccgaaggg????1080

gcttacagat?ttttgctgga?gtatgcggaa?ctgttgcgaa?gcgcaggatt?tgtggtcaag????1140

cttccctcgt?ggtggatcgg?tcgcagagga?gtcaaccgta?tcgggatcaa?gacaaaagtg????1200

aagcttccct?ctatgaaagg?aagcgggtcg?ggtctcacgc?tggatcgcat?ggttgcctgc????1260

gattatgctg?ctgcacttgg?caatgaggag?cttgacctgc?aggagctgaa?aacactggca????1320

aacctgaaag?ttccgctggt?acgggtgcgc?ggacagtgga?cacagattga?ccataaggag????1380

cttgccaatg?ctctccattt?tcttgaaaaa?catccaactg?gtgaactttc?tgccagagaa????1440

ctcctctcaa?cagctctcgg?agcacaaaaa?aaggaggatg?ctctctttct?tcgatcggtt????1500

gaaatcgagg?ggtggcttca?ggaactgctt?gaaaaacttt?cctctcaggg?acaatttgaa????1560

ctgcttccac?cacctgagca?tttcgaggga?acgcttcgcc?tctatcagga?gcgaggcttt????1620

tcatggctct?catttctccg?caagtgggga?ctgggcgcct?gtcttgccga?cgacatgggc????1680

cttggcaaaa?ccattcagac?gcttgcactg?ctgcagcggg?agcgtgaact?tggagaaaaa????1740

agggcggtgc?tcctgatctg?ccccacctct?gtagtcaaca?actggcgaaa?ggaggcggag????1800

cggttcactc?cggatttagc?ggtgctggtg?catcatggta?tcgaccggat?gaaaacagca????1860

gattttcgca?aagctgcaag?cgcttcagcc?cttgtcattt?caagctatgg?attgttacag????1920

cgcgaccttg?aatttctgtc?gaaggttccc?tgggcaggca?ttattctcga?tgaagcgcag????1980

aacatcaaaa?accctgagac?aaaacagtca?aaagctgccc?gaacaatccg?ggctgattac????2040

cgtattgccc?tgaccggcac?tcccgttgaa?aatcatgtcg?gcgacctttg?ggcactcatg????2100

gattttctca?atcccggttt?tcttggaacc?cagcactttt?tcaaacagaa?cttctacacg????2160

ccgattcagt?ggtatggcga?ccctgaggct?tcagcacgac?tgaagtcgct?gaccggcccg????2220

tttattctgc?gccgcatgaa?aagcgacaag?tcgattattt?ccgatctgcc?cgacaagatc????2280

gaaatgaaag?agtattgctc?gctgaccaaa?gagcaggcat?cgctctacaa?ggctgttgtc????2340

gatgaactgc?aggagaaaat?tgaaagcgcc?gaagggattg?accggcgggg?ccttgtactt????2400

gcgctgctgg?tcaagctcaa?gcaggtctgc?aaccatccgg?cacatttgct?tggcgacaac????2460

tctgccattg?cacatcgttc?aggaaaaata?aaacgcctga?ccgaactgct?tggcgacatc????2520

cgcgaagctg?gcgaaaaaac?gctgctcttt?acacagttta?ccatgatggg?aacgatgctc????2580

cagcactatc?ttcaggagtt?gtacggtgaa?gaggtactgt?ttctgcacgg?tggcgtaacc????2640

aaaaaaaggc?gggatgagat?ggtagagagc?ttccagaagg?aagagggcag?ttcaccctcc????2700

atctttattc?tctcactgaa?agccggagga?acgggtctta?acctgacaac?agcgaaccac????2760

gttgttcact?ttgaccgatg?gtggaacccg?gcagtagaga?atcaggcaac?tgaccgggct????2820

ttccgtatcg?ggcagcacaa?aaacgttgaa?gttcataaat?ttattacgac?gggcacgctc????2880

gaagagcgca?ttgatgagat?gattgagaaa?aaaacaacgg?tcgccggcca?ggttctcgga????2940

acgggtgagc?agtggctgac?cgaactgtcg?aacaatgatc?tgcgcaagct?cattatgctc????3000

ggacaggaag?caatgggaga?ataa?????????????????????3024

<210>66

<211>1007

<212>PRT

<213>Pelodictyon?phaeoclathratiforme

<400>66

Met?Ile?Ala?Leu?His?Ile?Ser?Ile?Ile?Asp?Gly?Val?Pro?Leu?Leu?Trp

1???????????????5???????????????????10??????????????????15

Ser?Glu?Gly?Lys?Lys?Ile?Gly?Met?Leu?Lys?Glu?Leu?Arg?Leu?Ala?Thr

20??????????????????25??????????????????30

Ala?Gly?Ile?Gly?Met?Phe?Ser?Leu?Leu?Asp?Asn?Thr?Thr?Lys?Glu?Phe

35??????????????????40??????????????????45

Cys?Val?Trp?Leu?Pro?Cys?Arg?Glu?Lys?Lys?Ala?Val?Pro?Ser?Ser?Pro

50??????????????????55??????????????????60

Leu?Val?Gly?Ala?Met?Pro?Asp?Leu?Ser?Asp?Glu?Glu?Gln?Leu?His?Ala

65??????????????????70??????????????????75??????????????????80

Phe?Pro?Ile?Thr?Ala?Leu?Arg?Leu?Asn?Phe?Asn?Ala?Leu?Phe?Glu?Leu

85??????????????????90??????????????????95

Ser?Leu?Leu?Thr?Glu?Lys?Gly?Asn?Ile?Pro?Gly?Ser?Gly?Ile?Ile?Phe

100?????????????????105?????????????????110

Gly?Ser?Ser?Leu?His?Trp?Ala?Arg?Gln?Val?Val?Lys?Ile?Ala?Leu?Asn

115?????????????????120?????????????????125

Ile?Val?Arg?Thr?Gln?Ser?Leu?Leu?Pro?Ser?Ile?Ile?Lys?Asn?Asp?Thr

130?????????????????135?????????????????140

Phe?Trp?Glu?Ala?Leu?Trp?Leu?Pro?Leu?Pro?Asp?Ser?Ala?Thr?Ser?Leu

145?????????????????150?????????????????155?????????????????160

Ala?Val?Glu?Gln?Leu?Ala?Asp?Ala?Met?Pro?Ala?Val?Cys?Arg?Ser?Leu

165?????????????????170?????????????????175

Gly?Arg?Thr?Asp?Thr?Gln?Pro?Pro?Glu?Thr?Pro?Lys?Lys?Leu?Leu?Leu

180?????????????????185?????????????????190

Lys?Gly?Leu?Leu?Ser?Phe?Leu?Val?Asn?Thr?Leu?Ser?Arg?Thr?Phe?Glu

195?????????????????200?????????????????205

Arg?Ala?Gly?Val?Pro?Lys?Ile?Ser?Asp?Phe?Glu?Ser?Ile?His?Asp?Ala

210?????????????????215?????????????????220

Trp?Leu?His?Ala?Leu?Ser?Asn?Ser?Asp?Pro?Arg?Leu?Lys?Trp?Lys?Asn

225?????????????????230?????????????????235?????????????????240

Glu?Gln?Glu?Ile?Glu?Gln?Phe?Ala?Cys?Gln?Leu?Asn?Ala?Trp?Arg?Arg

245?????????????????250?????????????????255

Pro?Ile?Asp?Leu?His?Glu?Arg?Ser?Pro?Phe?Arg?Phe?Cys?Leu?Gln?Leu

260?????????????????265?????????????????270

Thr?Glu?Pro?Pro?Leu?Lys?Gly?Arg?Lys?Lys?Glu?Arg?Trp?His?Val?Ala

275?????????????????280?????????????????285

Tyr?Gln?Leu?Gln?Leu?Lys?Ala?Asp?Pro?Ser?Leu?Ile?Leu?Asp?Ala?Gly

290?????????????????295?????????????????300

Asp?Leu?Trp?Asn?Pro?Glu?Ser?Glu?Ala?Ser?Gln?His?Ala?Leu?Thr?Tyr

305?????????????????310?????????????????315?????????????????320

Thr?Ser?Asp?Cys?Thr?Glu?Phe?Leu?Leu?Thr?Ser?Leu?Gly?Gln?Ala?Ser

325?????????????????330?????????????????335

Gly?Leu?Cys?Pro?Ala?Val?Thr?Gln?Ser?Leu?Lys?Lys?Lys?Gln?Pro?Gly

340?????????????????345?????????????????350

Gly?Phe?Asp?Leu?Asp?Thr?Glu?Gly?Ala?Tyr?Arg?Phe?Leu?Leu?Glu?Tyr

355?????????????????360?????????????????365

Ala?Glu?Leu?Leu?Arg?Ser?Ala?Gly?Phe?Val?Val?Lys?Leu?Pro?Ser?Trp

370?????????????????375?????????????????380

Trp?Ile?Gly?Arg?Arg?Gly?Val?Asn?Arg?Ile?Gly?Ile?Lys?Thr?Lys?Val

385?????????????????390?????????????????395?????????????????400

Lys?Leu?Pro?Ser?Met?Lys?Gly?Ser?Gly?Ser?Gly?Leu?Thr?Leu?Asp?Arg

405?????????????????410?????????????????415

Met?Val?Ala?Cys?Asp?Tyr?Ala?Ala?Ala?Leu?Gly?Asn?Glu?Glu?Leu?Asp

420?????????????????425?????????????????430

Leu?Gln?Glu?Leu?Lys?Thr?Leu?Ala?Asn?Leu?Lys?Val?Pro?Leu?Val?Arg

435?????????????????440?????????????????445

Val?Arg?Gly?Gln?Trp?Thr?Gln?Ile?Asp?His?Lys?Glu?Leu?Ala?Asn?Ala

450?????????????????455?????????????????460

Leu?His?Phe?Leu?Glu?Lys?His?Pro?Thr?Gly?Glu?Leu?Ser?Ala?Arg?Glu

465?????????????????470?????????????????475?????????????????480

Leu?Leu?Ser?Thr?Ala?Leu?Gly?Ala?Gln?Lys?Lys?Glu?Asp?Ala?Leu?Phe

485?????????????????490?????????????????495

Leu?Arg?Ser?Val?Glu?Ile?Glu?Gly?Trp?Leu?Gln?Glu?Leu?Leu?Glu?Lys

500?????????????????505?????????????????510

Leu?Ser?Ser?Gln?Gly?Gln?Phe?Glu?Leu?Leu?Pro?Pro?Pro?Glu?His?Phe

515?????????????????520?????????????????525

Glu?Gly?Thr?Leu?Arg?Leu?Tyr?Gln?Glu?Arg?Gly?Phe?Ser?Trp?Leu?Ser

530?????????????????535?????????????????540

Phe?Leu?Arg?Lys?Trp?Gly?Leu?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly

545?????????????????550?????????????????555?????????????????560

Leu?Gly?Lys?Thr?Ile?Gln?Thr?Leu?Ala?Leu?Leu?Gln?Arg?Glu?Arg?Glu

565?????????????????570?????????????????575

Leu?Gly?Glu?Lys?Arg?Ala?Val?Leu?Leu?Ile?Cys?Pro?Thr?Ser?Val?Val

580?????????????????585?????????????????590

Asn?Asn?Trp?Arg?Lys?Glu?Ala?Glu?Arg?Phe?Thr?Pro?Asp?Leu?Ala?Val

595?????????????????600?????????????????605

Leu?Val?His?His?Gly?Ile?Asp?Arg?Met?Lys?Thr?Ala?Asp?Phe?Arg?Lys

610?????????????????615?????????????????620

Ala?Ala?Ser?Ala?Ser?Ala?Leu?Val?Ile?Ser?Ser?Tyr?Gly?Leu?Leu?Gln

625?????????????????630?????????????????635?????????????????640

Arg?Asp?Leu?Glu?Phe?Leu?Ser?Lys?Val?Pro?Trp?Ala?Gly?Ile?Ile?Leu

645?????????????????650?????????????????655

Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn?Pro?Glu?Thr?Lys?Gln?Ser?Lys?Ala

660?????????????????665?????????????????670

Ala?Arg?Thr?Ile?Arg?Ala?Asp?Tyr?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro

675?????????????????680?????????????????685

Val?Glu?Asn?His?Val?Gly?Asp?Leu?Trp?Ala?Leu?Met?Asp?Phe?Leu?Asn

690?????????????????695?????????????????700

Pro?Gly?Phe?Leu?Gly?Thr?Gln?His?Phe?Phe?Lys?Gln?Asn?Phe?Tyr?Thr

705?????????????????710?????????????????715?????????????????720

Pro?Ile?Gln?Trp?Tyr?Gly?Asp?Pro?Glu?Ala?Ser?Ala?Arg?Leu?Lys?Ser

725?????????????????730?????????????????735

Leu?Thr?Gly?Pro?Phe?Ile?Leu?Arg?Arg?Met?Lys?Ser?Asp?Lys?Ser?Ile

740?????????????????745?????????????????750

Ile?Ser?Asp?Leu?Pro?Asp?Lys?Ile?Glu?Met?Lys?Glu?Tyr?Cys?Ser?Leu

755?????????????????760?????????????????765

Thr?Lys?Glu?Gln?Ala?Ser?Leu?Tyr?Lys?Ala?Val?Val?Asp?Glu?Leu?Gln

770?????????????????775?????????????????780

Glu?Lys?Ile?Glu?Ser?Ala?Glu?Gly?Ile?Asp?Arg?Arg?Gly?Leu?Val?Leu

785?????????????????790?????????????????795?????????????????800

Ala?Leu?Leu?Val?Lys?Leu?Lys?Gln?Val?Cys?Asn?His?Pro?Ala?His?Leu

805?????????????????810?????????????????815

Leu?Gly?Asp?Asn?Ser?Ala?Ile?Ala?His?Arg?Ser?Gly?Lys?Ile?Lys?Arg

820?????????????????825?????????????????830

Leu?Thr?Glu?Leu?Leu?Gly?Asp?Ile?Arg?Glu?Ala?Gly?Glu?Lys?Thr?Leu

835?????????????????840?????????????????845

Leu?Phe?Thr?Gln?Phe?Thr?Met?Met?Gly?Thr?Met?Leu?Gln?His?Tyr?Leu

850?????????????????855?????????????????860

Gln?Glu?Leu?Tyr?Gly?Glu?Glu?Val?Leu?Phe?Leu?His?Gly?Gly?Val?Thr

865?????????????????870?????????????????875?????????????????880

Lys?Lys?Arg?Arg?Asp?Glu?Met?Val?Glu?Ser?Phe?Gln?Lys?Glu?Glu?Gly

885?????????????????890?????????????????895

Ser?Ser?Pro?Ser?Ile?Phe?Ile?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Thr?Gly

900?????????????????905?????????????????910

Leu?Asn?Leu?Thr?Thr?Ala?Asn?His?Val?Val?His?Phe?Asp?Arg?Trp?Trp

915?????????????????920?????????????????925

Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp?Arg?Ala?Phe?Arg?Ile?Gly

930?????????????????935?????????????????940

Gln?His?Lys?Asn?Val?Glu?Val?His?Lys?Phe?Ile?Thr?Thr?Gly?Thr?Leu

945?????????????????950?????????????????955?????????????????960

Glu?Glu?Arg?Ile?Asp?Glu?Met?Ile?Glu?Lys?Lys?Thr?Thr?Val?Ala?Gly

965?????????????????970?????????????????975

Gln?Val?Leu?Gly?Thr?Gly?Glu?Gln?Trp?Leu?Thr?Glu?Leu?Ser?Asn?Asn

980?????????????????985?????????????????990

Asp?Leu?Arg?Lys?Leu?Ile?Met?Leu??Gly?Gln?Glu?Ala?Met??Gly?Glu

995?????????????????1000?????????????????1005

<210>67

<211>3189

<212>DNA

<213>Prochlorococcus?marinus

<400>67

atgactctgc?tgcacgccac?ttggatttca?actaattggc?atccatctaa?tttaggtcaa?????60

tcagaattgt?tcctttgggc?agaccaatgg?cgcgtagtaa?ctccaaaaca?aataatacaa????120

acaccttcac?ctcacccgtt?tagcctatct?tcagatgaat?taaaagaatg?gctcaatagc????180

aaaaaattat?tgcctaatga?gagtattaat?acatctgcat?gtctcactct?tcctagtaaa????240

cccattcaca?aaaaaaataa?ccaaaaatct?aagaatcaaa?aaactggtat?tgaatctgaa????300

tggaagggac?tccctttaca?agctcatgaa?gaaatagcaa?cacaatatga?atgttggcca????360

tggaaagtag?atggaatttc?actcactact?gtcgaagcaa?cagaatggct?tacaaaatta????420

cctttatcaa?aaaaagattc?tgatcttagt?gaagaattac?tttggtgggc?tcatttagag????480

cgttggtctc?ttaatctaat?tgcgagtgga?ctatggctac?ctcaagttaa?attacacaag????540

aaagaaggaa?atgaatatcg?tgcatcatgg?atacctctgc?tgaatcaaga?aaatgaaaga????600

aatcgcttag?aagagtttgc?aaaaaatatt?cccttggtcg?ctatttgtgc?agtcccatgg????660

atagaagcta?aaggacaaat?agtcaatact?gagcaagtct?caaattcaaa?caataataca????720

ctctctttat?ataggccaag?acacaatcgc?gtagaagtga?tggatcttct?cgaagaactt????780

attgatgcac?aacttcgaaa?agattttcaa?ccaagaacta?aaaacttgga?tccattgtta????840

aaagcgtggc?aagaagcact?tggcacgaaa?gatggaataa?ttaacctatc?gaatgaaaac????900

gctaaaagat?tagaaaaagc?aagtaagaat?tggaaaagag?ggttgtctag?taatgttcaa????960

cctgcgaaaa?catgtctaga?gctaattgca?ccgattgatg?atctagattt?atgggactta???1020

aacttttcat?tgcaatcaga?atcagatccg?agtatcagac?tagctgcaga?tcaaatttgg???1080

gaagcaggcg?tagaagtaac?caaagttggc?ggaataacaa?ttgacaaccc?aagtgaaatt???1140

cttttagaag?gcctaggaag?aagtcttgaa?attttccctc?caattgaaaa?aggactagaa???1200

agcccaactc?ctcacacaat?gaaactgtct?gcatcagaag?catttgtact?tattagaaca????1260

gcagcagcaa?aacttcgtga?catgggtatt?ggtgtaatac?tgcctaatag?tttgtccaaa????1320

ggatttgcaa?gtcgacttgg?tcttgctatt?caagccgaat?taccagagtc?ttcactaggc????1380

gtaatgctag?gagaaagttt?gaactgggat?tgggagttaa?tgatcggagg?tataaattta????1440

agcatgaaag?aactagaaat?gcttgcaaaa?aaaaatagtc?ctctactcaa?tcacaaaggg????1500

acatggatcg?aattacgtcc?taatgatctg?aaaaatgctt?caaaattttt?tgctaatact????1560

ccagaattaa?acctcgataa?agcattaagg?cttagtgcta?ataaaggcaa?cacttttatg????1620

aaacttccag?tacatcattt?tgaatctgga?ccaagattac?aaagtgtctt?agagcaatat????1680

caccatcaga?aagcgcctga?acctttacca?gcacctaatg?gattccatgg?gcaattaagg????1740

ccttaccaag?aaagaggtct?tgggtggctt?gcatttcttt?atcgttttaa?gcaaggagca????1800

tgcttagcag?atgacatggg?gcttggtaaa?actattcaat?tattatgttt?tattcagcac????1860

ctaaaagttc?aaaacgagct?tactaagcct?gtactcctaa?ttgcgcctac?atctgtgctg????1920

acaaattgga?aaagagaggc?tgccactttt?actccagaac?tatgtataca?tgaacactat????1980

ggtagtaaga?gacattcttc?aataccaaaa?ttacaaaatt?atctaaaaaa?agttgacatt????2040

atgatcacaa?gttatgggtt?actttatcga?gatggcgagc?tgctacaaga?aatcgactgg????2100

caaggaatag?ttattgatga?agctcaagct?attaaaaatt?ccaaatcaaa?gcaaagtatt????2160

ataactagag?caataagcaa?aaatctcata?agtaatccct?ttagaattgc?tttaacagga????2220

acgccagtag?aaaatcgtat?tagtgaacta?tgggcactaa?tggatttcct?taatccaaaa????2280

gtattaggtg?aagaagattt?ttttaatcag?cgatacaagt?taccgattga?gcattatggc????2340

gacatctctt?cattaaaaga?tctcaaaaca?caggtcagtc?cttttatttt?aagaagattg????2400

aaaaccgatc?aatctattat?ttctgatttg?cctcaaaaga?ttgaattaaa?tgagtgggtt????2460

ggactaagcc?aagagcaaga?gcttctatat?aaacaaacgg?tagagaaaag?cttagatgaa????2520

ctcgcctcat?tacccattgg?tcaacgccag?ggtaaaacat?tgggtctact?tactcgtctt????2580

aaacaaattt?gtaatcatcc?agcaattgct?ttaaaagaaa?ctcaagtcga?gaagaatttc????2640

ttattaagat?cttcaaaatt?acaaagactg?gaagaaatac?tacaagaagt?gaaagaatct????2700

catgatagag?ctctgctctt?tactcaattt?gctgaatggg?ggcatttatt?gcaagcgtac????2760

ttacaaacaa?aatgggaatc?agaagtacct?ttcctacacg?gaggcactcc?taaagggaag????2820

cgacaagaaa?tgatagatcg?ttttcaagat?gatcctagag?ggccaaatat?ctttttactt????2880

tcactaaaag?caggaggagt?gggtcttaat?ctaactcgtg?cgaatcatgt?ttttcatatt????2940

gatcgttggt?ggaatccagc?agtagaaaat?caagcaacag?atcgtgcata?ccgaattggt????3000

caaaaaaaaa?gtgttatcgt?ccataagttt?ataaccaccg?gcacaatcga?agaaaaaatc????3060

aatcaaatga?ttctcgaaaa?gactgaacta?gcagaaaata?ttgtcggatc?aggagaaagc????3120

tggttagggc?aattaagtct?tgaaaaattg?agtgaattag?ttgctttaga?tagcaatcca????3180

gaattctaa????????????????????????????????????????????????????????????3189

<210>68

<211>1062

<212>PRT

<213>Prochlorococcus?marinus

<400>68

Met?Thr?Leu?Leu?His?Ala?Thr?Trp?Ile?Ser?Thr?Asn?Trp?His?Pro?Ser

1???????????????5???????????????????10??????????????????15

Asn?Leu?Gly?Gln?Ser?Glu?Leu?Phe?Leu?Trp?Ala?Asp?Gln?Trp?Arg?Val

20??????????????????25??????????????????30

Val?Thr?Pro?Lys?Gln?Ile?Ile?Gln?Thr?Pro?Ser?Pro?His?Pro?Phe?Ser

35??????????????????40??????????????????45

Leu?Ser?Ser?Asp?Glu?Leu?Lys?Glu?Trp?Leu?Asn?Ser?Lys?Lys?Leu?Leu

50??????????????????55??????????????????60

Pro?Asn?Glu?Ser?Ile?Asn?Thr?Ser?Ala?Cys?Leu?Thr?Leu?Pro?Ser?Lys

65??????????????????70??????????????????75??????????????????80

Pro?Ile?His?Lys?Lys?Asn?Asn?Gln?Lys?Ser?Lys?Asn?Gln?Lys?Thr?Gly

85??????????????????90??????????????????95

Ile?Glu?Ser?Glu?Trp?Lys?Gly?Leu?Pro?Leu?Gln?Ala?His?Glu?Glu?Ile

100?????????????????105?????????????????110

Ala?Thr?Gln?Tyr?Glu?Cys?Trp?Pro?Trp?Lys?Val?Asp?Gly?Ile?Ser?Leu

115?????????????????120?????????????????125

Thr?Thr?Val?Glu?Ala?Thr?Glu?Trp?Leu?Thr?Lys?Leu?Pro?Leu?Ser?Lys

130?????????????????135?????????????????140

Lys?Asp?Ser?Asp?Leu?Ser?Glu?Glu?Leu?Leu?Trp?Trp?Ala?His?Leu?Glu

145?????????????????150?????????????????155?????????????????160

Arg?Trp?Ser?Leu?Asn?Leu?Ile?Ala?Ser?Gly?Leu?Trp?Leu?Pro?Gln?Val

165?????????????????170?????????????????175

Lys?Leu?His?Lys?Lys?Glu?Gly?Asn?Glu?Tyr?Arg?Ala?Ser?Trp?Ile?Pro

180?????????????????185?????????????????190

Leu?Leu?Asn?Gln?Glu?Asn?Glu?Arg?Asn?Arg?Leu?Glu?Glu?Phe?Ala?Lys

195?????????????????200?????????????????205

Asn?Ile?Pro?Leu?Val?Ala?Ile?Cys?Ala?Val?Pro?Trp?Ile?Glu?Ala?Lys

210?????????????????215?????????????????220

Gly?Gln?Ile?Val?Asn?Thr?Glu?Gln?Val?Ser?Asn?Ser?Asn?Asn?Asn?Thr

225?????????????????230?????????????????235?????????????????240

Leu?Ser?Leu?Tyr?Arg?Pro?Arg?His?Asn?Arg?Val?Glu?Val?Met?Asp?Leu

245?????????????????250?????????????????255

Leu?Glu?Glu?Leu?Ile?Asp?Ala?Gln?Leu?Arg?Lys?Asp?Phe?Gln?Pro?Arg

260?????????????????265?????????????????270

Thr?Lys?Asn?Leu?Asp?Pro?Leu?Leu?Lys?Ala?Trp?Gln?Glu?Ala?Leu?Gly

275?????????????????280?????????????????285

Thr?Lys?Asp?Gly?Ile?Ile?Asn?Leu?Ser?Asn?Glu?Asn?Ala?Lys?Arg?Leu

290?????????????????295?????????????????300

Glu?Lys?Ala?Ser?Lys?Asn?Trp?Lys?Arg?Gly?Leu?Ser?Ser?Asn?Val?Gln

305?????????????????310?????????????????315?????????????????320

Pro?Ala?Lys?Thr?Cys?Leu?Glu?Leu?Ile?Ala?Pro?Ile?Asp?Asp?Leu?Asp

325?????????????????330?????????????????335

Leu?Trp?Asp?Leu?Asn?Phe?Ser?Leu?Gln?Ser?Glu?Ser?Asp?Pro?Ser?Ile

340?????????????????345?????????????????350

Arg?Leu?Ala?Ala?Asp?Gln?Ile?Trp?Glu?Ala?Gly?Val?Glu?Val?Thr?Lys

355?????????????????360?????????????????365

Val?Gly?Gly?Ile?Thr?Ile?Asp?Asn?Pro?Ser?Glu?Ile?Leu?Leu?Glu?Gly

370?????????????????375?????????????????380

Leu?Gly?Arg?Ser?Leu?Glu?Ile?Phe?Pro?Pro?Ile?Glu?Lys?Gly?Leu?Glu

385?????????????????390?????????????????395?????????????????400

Ser?Pro?Thr?Pro?His?Thr?Met?Lys?Leu?Ser?Ala?Ser?Glu?Ala?Phe?Val

405?????????????????410?????????????????415

Leu?Ile?Arg?Thr?Ala?Ala?Ala?Lys?Leu?Arg?Asp?Met?Gly?Ile?Gly?Val

420?????????????????425?????????????????430

Ile?Leu?Pro?Asn?Ser?Leu?Ser?Lys?Gly?Phe?Ala?Ser?Arg?Leu?Gly?Leu

435?????????????????440?????????????????445

Ala?Ile?Gln?Ala?Glu?Leu?Pro?Glu?Ser?Ser?Leu?Gly?Val?Met?Leu?Gly

450?????????????????455?????????????????460

Glu?Ser?Leu?Asn?Trp?Asp?Trp?Glu?Leu?Met?Ile?Gly?Gly?Ile?Asn?Leu

465?????????????????470?????????????????475?????????????????480

Ser?Met?Lys?Glu?Leu?Glu?Met?Leu?Ala?Lys?Lys?Asn?Ser?Pro?Leu?Leu

485?????????????????490?????????????????495

Asn?His?Lys?Gly?Thr?Trp?Ile?Glu?Leu?Arg?Pro?Asn?Asp?Leu?Lys?Asn

500?????????????????505?????????????????510

Ala?Ser?Lys?Phe?Phe?Ala?Asn?Thr?Pro?Glu?Leu?Asn?Leu?Asp?Lys?Ala

515?????????????????520?????????????????525

Leu?Arg?Leu?Ser?Ala?Asn?Lys?Gly?Asn?Thr?Phe?Met?Lys?Leu?Pro?Val

530?????????????????535?????????????????540

His?His?Phe?Glu?Ser?Gly?Pro?Arg?Leu?Gln?Ser?Val?Leu?Glu?Gln?Tyr

545?????????????????550?????????????????555?????????????????560

His?His?Gln?Lys?Ala?Pro?Glu?Pro?Leu?Pro?Ala?Pro?Asn?Gly?Phe?His

565?????????????????570?????????????????575

Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Leu?Gly?Trp?Leu?Ala?Phe

580?????????????????585?????????????????590

Leu?Tyr?Arg?Phe?Lys?Gln?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu

595?????????????????600?????????????????605

Gly?Lys?Thr?Ile?Gln?Leu?Leu?Cys?Phe?Ile?Gln?His?Leu?Lys?Val?Gln

610?????????????????615?????????????????620

Asn?Glu?Leu?Thr?Lys?Pro?Val?Leu?Leu?Ile?Ala?Pro?Thr?Ser?Val?Leu

625?????????????????630?????????????????635?????????????????640

Thr?Asn?Trp?Lys?Arg?Glu?Ala?Ala?Thr?Phe?Thr?Pro?Glu?Leu?Cys?Ile

645?????????????????650?????????????????655

His?Glu?His?Tyr?Gly?Ser?Lys?Arg?His?Ser?Ser?Ile?Pro?Lys?Leu?Gln

660?????????????????665?????????????????670

Asn?Tyr?Leu?Lys?Lys?Val?Asp?Ile?Met?Ile?Thr?Ser?Tyr?Gly?Leu?Leu

675?????????????????680?????????????????685

Tyr?Arg?Asp?Gly?Glu?Leu?Leu?Gln?Glu?Ile?Asp?Trp?Gln?Gly?Ile?Val

690?????????????????695?????????????????700

Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys?Asn?Ser?Lys?Ser?Lys?Gln?Ser?Ile

705?????????????????710?????????????????715?????????????????720

Ile?Thr?Arg?Ala?Ile?Ser?Lys?Asn?Leu?Ile?Ser?Asn?Pro?Phe?Arg?Ile

725?????????????????730?????????????????735

Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Ile?Ser?Glu?Leu?Trp?Ala

740?????????????????745?????????????????750

Leu?Met?Asp?Phe?Leu?Asn?Pro?Lys?Val?Leu?Gly?Glu?Glu?Asp?Phe?Phe

755?????????????????760?????????????????765

Asn?Gln?Arg?Tyr?Lys?Leu?Pro?Ile?Glu?His?Tyr?Gly?Asp?Ile?Ser?Ser

770?????????????????775?????????????????780

Leu?Lys?Asp?Leu?Lys?Thr?Gln?Val?Ser?Pro?Phe?Ile?Leu?Arg?Arg?Leu

785?????????????????790?????????????????795?????????????????800

Lys?Thr?Asp?Gln?Ser?Ile?Ile?Ser?Asp?Leu?Pro?Gln?Lys?Ile?Glu?Leu

805?????????????????810?????????????????815

Asn?Glu?Trp?Val?Gly?Leu?Ser?Gln?Glu?Gln?Glu?Leu?Leu?Tyr?Lys?Gln

820?????????????????825?????????????????830

Thr?Val?Glu?Lys?Ser?Leu?Asp?Glu?Leu?Ala?Ser?Leu?Pro?Ile?Gly?Gln

835?????????????????840?????????????????845

Arg?Gln?Gly?Lys?Thr?Leu?Gly?Leu?Leu?Thr?Arg?Leu?Lys?Gln?Ile?Cys

850?????????????????855?????????????????860

Asn?His?Pro?Ala?Ile?Ala?Leu?Lys?Glu?Thr?Gln?Val?Glu?Lys?Asn?Phe

865?????????????????870?????????????????875?????????????????880

Leu?Leu?Arg?Ser?Ser?Lys?Leu?Gln?Arg?Leu?Glu?Glu?Ile?Leu?Gln?Glu

885?????????????????890?????????????????895

Val?Lys?Glu?Ser?His?Asp?Arg?Ala?Leu?Leu?Phe?Thr?Gln?Phe?Ala?Glu

900?????????????????905?????????????????910

Trp?Gly?His?Leu?Leu?Gln?Ala?Tyr?Leu?Gln?Thr?Lys?Trp?Glu?Ser?Glu

915?????????????????920?????????????????925

Val?Pro?Phe?Leu?His?Gly?Gly?Thr?Pro?Lys?Gly?Lys?Arg?Gln?Glu?Met

930?????????????????935?????????????????940

Ile?Asp?Arg?Phe?Gln?Asp?Asp?Pro?Arg?Gly?Pro?Asn?Ile?Phe?Leu?Leu

945?????????????????950?????????????????955?????????????????960

Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr?Arg?Ala?Asn?His

965?????????????????970?????????????????975

Val?Phe?His?Ile?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala

980?????????????????985?????????????????990

Thr?Asp?Arg?Ala?Tyr?Arg?Ile?Gly??Gln?Lys?Lys?Ser?Val??Ile?Val?His

995?????????????????1000?????????????????1005

Lys?Phe??Ile?Thr?Thr?Gly?Thr??Ile?Glu?Glu?Lys?Ile??Asn?Gln?Met

1010?????????????????1015?????????????????1020

Ile?Leu??Glu?Lys?Thr?Glu?Leu??Ala?Glu?Asn?Ile?Val??Gly?Ser?Gly

1025?????????????????1030?????????????????1035

Glu?Ser??Trp?Leu?Gly?Gln?Leu??Ser?Leu?Glu?Lys?Leu??Ser?Glu?Leu

1040?????????????????1045?????????????????1050

Val?Ala??Leu?Asp?Ser?Asn?Pro??Glu?Phe

1055?????????????????1060

<210>69

<211>3204

<212>DNA

<213>Prochlorococcus?marinus

<400>69

atgagtctgc?tacacgctac?ttggctgcca?gcaatgcgaa?ccggaagttc?gcataatcca????60

ggactactca?tctgggctga?ttcatggaga?gttgcaaaac?caagcatagt?cagcaatcag????120

cctgtaatac?atccatttgc?cttatcagca?gcagatttac?gtatttggct?attgcaaaaa????180

aagcttttac?ctaaagaaag?tattgaatgt?acagccttat?taactctacc?tagtaaatct????240

attaaaaact?cattagacaa?aaaattaaat?ggagtaacgg?actcacaaaa?tactagcgat????300

caacctcaat?ggagtggact?acctttacaa?gcaggagagc?cagtaactaa?acaatgtgaa????360

tggtggccct?ggcaagttga?aggtatagca?atcaaaccca?gtgaagctgc?atcgtggctt????420

gcaaacttac?ctctcacgaa?aaaagatcct?gagcttagtg?aagagatcct?atggtggagt????480

catttagaac?gttggtctct?aagtttaatt?gctcgtggcc?tttggttgcc?acaagttgaa????540

ttaaatacaa?ttgataatat?tggagctaga?gctaggtgga?gtcctttact?taataacgaa????600

aacgagcgca?aaagattaga?agaattctct?atcaggcttc?cattagtagc?aacatgtgcc????660

ataaaaagag?aggaaacttc?tgaagaaaat?caaaaccata?tattaaagac?tactcctagg????720

gaaacactcg?atgaatacgg?acttgcagta?tgtcgaccaa?tcaatagtcg?acttcaagtg????780

gcttatctct?tagaagaact?cgtggatgga?cagctaagaa?aagattttga?ggaaagttct????840

gaagaccttg?atccattgct?gaaagcttgg?caagaggcat?taggatcaca?taatggagtc????900

attcgtcttc?cgttggaaga?ttgtgaaaga?ttagccaagg?caagtaaaaa?ttggaaagaa????960

aatttatcag?gcaatgttaa?aggtgcaaga?gcatgccttg?agctttttgc?accacttgaa???1020

ggagaagatt?tatgggactt?acaattctct?ttacaagctg?aagcagatcc?atcactaaag???1080

gtagcagcag?aagcagtatg?gaatgcagac?tcagcagttc?tacagattgg?tgatattcaa???1140

atagcgcagc?ctggagaaat?tctactagaa?ggtcttggca?gagcactcaa?tatctttcaa???1200

ccaatagaaa?ggggtctgga?aaatgctact?ccaaataata?tgcaactcac?acctgcagaa???1260

gcttttgttc?tagtacgtac?agcctcaaag?caattacgtg?atattggtat?tggtgtaata???1320

ctacctagaa?gtttatcagg?aggattagca?agtcgactag?gtatagctat?taaagcagag???1380

ttagcgacta?gtgccagagg?attaacactt?cgagagaatc?tagaatggag?ttgggagcta????1440

atgatagggg?gaagcatatt?aagccttaaa?gatctagaac?aactggcaag?taaacgcagc????1500

cctctagttc?gctataagga?ttcatggctt?gaattacgtc?caaatgatct?taaaatcgcc????1560

gaaaaattct?gtagcaataa?tcctgaatta?agcctagatg?acgcattaag?acttaccgca????1620

actaaagggg?agactctaat?gaagcttcca?gtacatcaat?ttaatgctgg?gccaaagctc????1680

caaggcgttt?tagagcaata?ccaccaacat?acaagtcctg?agcctctagc?tgcaccagat????1740

ggcttctatg?gacaactgag?gccttatcaa?gaacgtggca?taggatggtt?ggctttcttg????1800

catcgtttta?atcaaggtgc?atgtttagca?gatgacatgg?gcctgggcaa?aacaattcaa????1860

gtgcttgctt?ttattcagca?cttaaaaagt?aacaaggacc?tcaagaaacc?tgttttgcta????1920

attgcaccta?cgtcagtatt?aacaaactgg?aaacgagaag?cttattcatt?tacaccagag????1980

ttatctgtat?tagagcatta?cggtcctaat?cgttcatcta?catcaacact?cttgaaaaag????2040

attctcaaaa?aagtagacat?tcttattact?agctatggcc?tactacatag?agataaacag????2100

cttctgaaaa?caattgattg?gcaaggtgta?attattgatg?aagcacaagc?tataaaaaat????2160

ccaaattcaa?aacaaagtca?aacaactcgt?gaaattgtta?aaggcggaaa?aataatccct????2220

tttcgtattg?cattaactgg?tacccctata?gaaaatcgtg?taagtgagct?ttggtcatta????2280

atggattttt?taaatccatc?agtacttgga?gaaaaagaat?tttttgatca?acgctacaaa????2340

ttaccgattg?aacgttatgg?tgatatttct?tcgttaaccg?atctcaaagc?tcgtgtcagt????2400

ccctttattc?ttagaaggtt?aaaaagtgat?aaatcaatta?tctcggatct?accaagcaaa????2460

gtcgaactaa?aagaatggat?tactcttagt?caagagcaaa?gagctcttta?taacaaaact????2520

gtagacaata?ccttacagga?aatcgcaaga?agtcctattg?gtcagcgtca?tgcgaaaacc????2580

ttaggtctat?taacacgtct?caaacaaata?tgtaatcatc?ctgctcttgc?cctcaaagaa????2640

aaaaacatta?gcgatgattt?tggaatacga?tcaaccaaac?ttcaaaggct?ggaagaactt????2700

cttgatgtga?tattcgcaac?agaggacaga?gctcttcttt?ttacccaatt?cgctgaatgg????2760

ggtcacttac?tacaagctta?tctagaaaaa?aagtggggac?atagcatact?ttttctacat????2820

ggaggaactc?gcaaaataga?tagacaatca?atggttgatc?aatttcaaga?agatcccaga????2880

ggcccaaaat?tatttttact?ttctctcaaa?gcaggtggta?ttggtctgaa?cctgactcga????2940

gctaaccacg?tgttgcatat?tgatcgatgg?tggaaccctg?ccgtagaaaa?tcaggcaaca????3000

gatcgtgctt?atagaattgg?tcaaaaaaat?agcgtaatgg?ttcacaaatt?tattgctaca????3060

gggtcagtag?aagaaaaaat?tgatcaaatg?attactgaaa?agtctaagct?cgcagaaaat????3120

ataattggtg?caggtgaaga?ttggcttggc?aaacttggca?tcaatgaatt?acgtgaatta????3180

gtttccttag?aaaaagagag?ttaa???????????????????????????????????????????3204

<210>70

<211>1067

<212>PRT

<213>Prochlorococcus?marinus

<400>70

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Pro?Ala?Met?Arg?Thr?Gly?Ser

1???????????????5???????????????????10??????????????????15

Ser?His?Asn?Pro?Gly?Leu?Leu?Ile?Trp?Ala?Asp?Ser?Trp?Arg?Val?Ala

20??????????????????25??????????????????30

Lys?Pro?Ser?Ile?Val?Ser?Asn?Gln?Pro?Val?Ile?His?Pro?Phe?Ala?Leu

35??????????????????40??????????????????45

Ser?Ala?Ala?Asp?Leu?Arg?Ile?Trp?Leu?Leu?Gln?Lys?Lys?Leu?Leu?Pro

50??????????????????55??????????????????60

Lys?Glu?Ser?Ile?Glu?Cys?Thr?Ala?Leu?Leu?Thr?Leu?Pro?Ser?Lys?Ser

65??????????????????70??????????????????75??????????????????80

Ile?Lys?Asn?Ser?Leu?Asp?Lys?Lys?Leu?Asn?Gly?Val?Thr?Asp?Ser?Gln

85??????????????????90??????????????????95

Asn?Thr?Ser?Asp?Gln?Pro?Gln?Trp?Ser?Gly?Leu?Pro?Leu?Gln?Ala?Gly

100?????????????????105?????????????????110

Glu?Pro?Val?Thr?Lys?Gln?Cys?Glu?Trp?Trp?Pro?Trp?Gln?Val?Glu?Gly

115?????????????????120?????????????????125

Ile?Ala?Ile?Lys?Pro?Ser?Glu?Ala?Ala?Ser?Trp?Leu?Ala?Asn?Leu?Pro

130?????????????????135?????????????????140

Leu?Thr?Lys?Lys?Asp?Pro?Glu?Leu?Ser?Glu?Glu?Ile?Leu?Trp?Trp?Ser

145?????????????????150?????????????????155?????????????????160

His?Leu?Glu?Arg?Trp?Ser?Leu?Ser?Leu?Ile?Ala?Arg?Gly?Leu?Trp?Leu

165?????????????????170?????????????????175

Pro?Gln?Val?Glu?Leu?Asn?Thr?Ile?Asp?Asn?Ile?Gly?Ala?Arg?Ala?Arg

180?????????????????185?????????????????190

Trp?Ser?Pro?Leu?Leu?Asn?Asn?Glu?Asn?Glu?Arg?Lys?Arg?Leu?Glu?Glu

195?????????????????200?????????????????205

Phe?Ser?Ile?Arg?Leu?Pro?Leu?Val?Ala?Thr?Cys?Ala?Ile?Lys?Arg?Glu

210?????????????????215?????????????????220

Glu?Thr?Ser?Glu?Glu?Asn?Gln?Asn?His?Ile?Leu?Lys?Thr?Thr?Pro?Arg

225?????????????????230?????????????????235?????????????????240

Glu?Thr?Leu?Asp?Glu?Tyr?Gly?Leu?Ala?Val?Cys?Arg?Pro?Ile?Asn?Ser

245?????????????????250?????????????????255

Arg?Leu?Gln?Val?Ala?Tyr?Leu?Leu?Glu?Glu?Leu?Val?Asp?Gly?Gln?Leu

260?????????????????265?????????????????270

Arg?Lys?Asp?Phe?Glu?Glu?Ser?Ser?Glu?Asp?Leu?Asp?Pro?Leu?Leu?Lys

275?????????????????280?????????????????285

Ala?Trp?Gln?Glu?Ala?Leu?Gly?Ser?His?Asn?Gly?Val?Ile?Arg?Leu?Pro

290?????????????????295?????????????????300

Leu?Glu?Asp?Cys?Glu?Arg?Leu?Ala?Lys?Ala?Ser?Lys?Asn?Trp?Lys?Glu

305?????????????????310?????????????????315?????????????????320

Asn?Leu?Ser?Gly?Asn?Val?Lys?Gly?Ala?Arg?Ala?Cys?Leu?Glu?Leu?Phe

325?????????????????330?????????????????335

Ala?Pro?Leu?Glu?Gly?Glu?Asp?Leu?Trp?Asp?Leu?Gln?Phe?Ser?Leu?Gln

340?????????????????345?????????????????350

Ala?Glu?Ala?Asp?Pro?Ser?Leu?Lys?Val?Ala?Ala?Glu?Ala?Val?Trp?Asn

355?????????????????360?????????????????365

Ala?Asp?Ser?Ala?Val?Leu?Gln?Ile?Gly?Asp?Ile?Gln?Ile?Ala?Gln?Pro

370?????????????????375?????????????????380

Gly?Glu?Ile?Leu?Leu?Glu?Gly?Leu?Gly?Arg?Ala?Leu?Asn?Ile?Phe?Gln

385?????????????????390?????????????????395?????????????????400

Pro?Ile?Glu?Arg?Gly?Leu?Glu?Asn?Ala?Thr?Pro?Asn?Asn?Met?Gln?Leu

405?????????????????410?????????????????415

Thr?Pro?Ala?Glu?Ala?Phe?Val?Leu?Val?Arg?Thr?Ala?Ser?Lys?Gln?Leu

420?????????????????425?????????????????430

Arg?Asp?Ile?Gly?Ile?Gly?Val?Ile?Leu?Pro?Arg?Ser?Leu?Ser?Gly?Gly

435?????????????????440?????????????????445

Leu?Ala?Ser?Arg?Leu?Gly?Ile?Ala?Ile?Lys?Ala?Glu?Leu?Ala?Thr?Ser

450?????????????????455?????????????????460

Ala?Arg?Gly?Leu?Thr?Leu?Arg?Glu?Asn?Leu?Glu?Trp?Ser?Trp?Glu?Leu

465?????????????????470?????????????????475?????????????????480

Met?Ile?Gly?Gly?Ser?Ile?Leu?Ser?Leu?Lys?Asp?Leu?Glu?Gln?Leu?Ala

485?????????????????490?????????????????495

Ser?Lys?Arg?Ser?Pro?Leu?Val?Arg?Tyr?Lys?Asp?Ser?Trp?Leu?Glu?Leu

500?????????????????505?????????????????510

Arg?Pro?Asn?Asp?Leu?Lys?Ile?Ala?Glu?Lys?Phe?Cys?Ser?Asn?Asn?Pro

515?????????????????520?????????????????525

Glu?Leu?Ser?Leu?Asp?Asp?Ala?Leu?Arg?Leu?Thr?Ala?Thr?Lys?Gly?Glu

530?????????????????535?????????????????540

Thr?Leu?Met?Lys?Leu?Pro?Val?His?Gln?Phe?Asn?Ala?Gly?Pro?Lys?Leu

545?????????????????550?????????????????555?????????????????560

Gln?Gly?Val?Leu?Glu?Gln?Tyr?His?Gln?His?Thr?Ser?Pro?Glu?Pro?Leu

565?????????????????570?????????????????575

Ala?Ala?Pro?Asp?Gly?Phe?Tyr?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu?Arg

580?????????????????585?????????????????590

Gly?Ile?Gly?Trp?Leu?Ala?Phe?Leu?His?Arg?Phe?Asn?Gln?Gly?Ala?Cys

595?????????????????600?????????????????605

Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Val?Leu?Ala?Phe

610?????????????????615?????????????????620

Ile?Gln?His?Leu?Lys?Ser?Asn?Lys?Asp?Leu?Lys?Lys?Pro?Val?Leu?Leu

625?????????????????630?????????????????635?????????????????640

Ile?Ala?Pro?Thr?Ser?Val?Leu?Thr?Asn?Trp?Lys?Arg?Glu?Ala?Tyr?Ser

645?????????????????650?????????????????655

Phe?Thr?Pro?Glu?Leu?Ser?Val?Leu?Glu?His?Tyr?Gly?Pro?Asn?Arg?Ser

660?????????????????665?????????????????670

Ser?Thr?Ser?Thr?Leu?Leu?Lys?Lys?Ile?Leu?Lys?Lys?Val?Asp?Ile?Leu

675?????????????????680?????????????????685

Ile?Thr?Ser?Tyr?Gly?Leu?Leu?His?Arg?Asp?Lys?Gln?Leu?Leu?Lys?Thr

690?????????????????695?????????????????700

Ile?Asp?Trp?Gln?Gly?Val?Ile?Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys?Asn

705?????????????????710?????????????????715?????????????????720

Pro?Asn?Ser?Lys?Gln?Ser?Gln?Thr?Thr?Arg?Glu?Ile?Val?Lys?Gly?Gly

725?????????????????730?????????????????735

Lys?Ile?Ile?Pro?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Ile?Glu?Asn

740?????????????????745?????????????????750

Arg?Val?Ser?Glu?Leu?Trp?Ser?Leu?Met?Asp?Phe?Leu?Asn?Pro?Ser?Val

755?????????????????760?????????????????765

Leu?Gly?Glu?Lys?Glu?Phe?Phe?Asp?Gln?Arg?Tyr?Lys?Leu?Pro?Ile?Glu

770?????????????????775?????????????????780

Arg?Tyr?Gly?Asp?Ile?Ser?Ser?Leu?Thr?Asp?Leu?Lys?Ala?Arg?Val?Ser

785?????????????????790?????????????????795?????????????????800

Pro?Phe?Ile?Leu?Arg?Arg?Leu?Lys?Ser?Asp?Lys?Ser?Ile?Ile?Ser?Asp

805?????????????????810?????????????????815

Leu?Pro?Ser?Lys?Val?Glu?Leu?Lys?Glu?Trp?Ile?Thr?Leu?Ser?Gln?Glu

820?????????????????825?????????????????830

Gln?Arg?Ala?Leu?Tyr?Asn?Lys?Thr?Val?Asp?Asn?Thr?Leu?Gln?Glu?Ile

835?????????????????840?????????????????845

Ala?Arg?Ser?Pro?Ile?Gly?Gln?Arg?His?Ala?Lys?Thr?Leu?Gly?Leu?Leu

850?????????????????855?????????????????860

Thr?Arg?Leu?Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Leu?Ala?Leu?Lys?Glu

865?????????????????870?????????????????875?????????????????880

Lys?Asn?Ile?Ser?Asp?Asp?Phe?Gly?Ile?Arg?Ser?Thr?Lys?Leu?Gln?Arg

885?????????????????890?????????????????895

Leu?Glu?Glu?Leu?Leu?Asp?Val?Ile?Phe?Ala?Thr?Glu?Asp?Arg?Ala?Leu

900?????????????????905?????????????????910

Leu?Phe?Thr?Gln?Phe?Ala?Glu?Trp?Gly?His?Leu?Leu?Gln?Ala?Tyr?Leu

915?????????????????920?????????????????925

Glu?Lys?Lys?Trp?Gly?His?Ser?Ile?Leu?Phe?Leu?His?Gly?Gly?Thr?Arg

930?????????????????935?????????????????940

Lys?Ile?Asp?Arg?Gln?Ser?Met?Val?Asp?Gln?Phe?Gln?Glu?Asp?Pro?Arg

945?????????????????950?????????????????955?????????????????960

Gly?Pro?Lys?Leu?Phe?Leu?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Ile?Gly?Leu

965?????????????????970?????????????????975

Asn?Leu?Thr?Arg?Ala?Asn?His?Val?Leu?His?Ile?Asp?Arg?Trp?Trp?Asn

980?????????????????985?????????????????990

Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr??Asp?Arg?Ala?Tyr?Arg??Ile?Gly?Gln

995?????????????????1000?????????????????1005

Lys?Asn??Ser?Val?Met?Val?His??Lys?PheIle?Ala?Thr??Gly?Ser?Val

1010?????????????????1015?????????????????1020

Glu?Glu??Lys?Ile?Asp?Gln?Met??Ile?Thr?Glu?Lys?Ser??Lys?Leu?Ala

1025?????????????????1030?????????????????1035

Glu?Asn??Ile?Ile?Gly?Ala?Gly??Glu?Asp?Trp?Leu?Gly??Lys?Leu?Gly

1040?????????????????1045?????????????????1050

Ile?Asn??Glu?Leu?Arg?Glu?Leu??Val?Ser?Leu?Glu?Lys??Glu?Ser

1055?????????????????1060?????????????????1065

<210>71

<211>3300

<212>DNA

<213>Prochlorococcus?marinus

<400>71

atgattggtt?gtggaactcc?tgcgtggatg?gttgccgttg?atcggcagtg?cactcctgct?????60

ccaagaaacc?caacacatac?tttttgcgtc?gcggccatga?gcctgctgca?cgccacctgg????120

cttccagcca?tccgtactcc?gaccagctcc?ggtcgccctg?cgctccttgt?gtgggcagat????180

acctggcgag?tcgctacccc?agcaggacca?gcagcaactc?ccgcactcca?ccccttcaca????240

ctcaacccag?acgatctacg?tgcctggctg?attgagcgcg?atctactgcc?cgatgaaatc????300

atcgacgcca?cagcatgtct?gaccctgcct?agccgaacag?tcaaaccgcg?cagcaaagcc????360

aagaacgtat?ccactgaatc?cgacgaagac?aaagaccaca?aaacaagttg?gacaggactg????420

cccttacaag?caggcgaacc?cattcccaaa?cagactgaat?ggtggccctg?gcaggtgcaa????480

ggcctggcag?tggagcctgc?tgctgcaacg?gcctggcttt?cgaaactgcc?tctttcagga????540

gatcatcctg?atctcgccga?tgaattgcgc?tggtggagcc?atctacagcg?ctgggccctg????600

agcatgattg?ctcgcggacg?ttggctaccc?caggtggaac?tcagcaaggg?agagggctat????660

ccccaccgag?cacgctggac?accgctactc?aaccgtgaag?atgatcgccg?ccgcctcgaa????720

gaccttgccg?ctcagctccc?cttagtggcc?acctgcgccc?tcccctggcg?ggagcccacc????780

ggaaggcgta?gcaaccgaat?gacccgccta?agaccagagg?cgatgcgagc?cgctaaccct????840

gtggcttcat?gccgaccccg?cagcggtcgc?cttcgcgtag?ccagcctgct?ggaagaactc????900

ttggatgccc?aactgcgcac?cggatttgaa?gcgagtgagc?aaggcctaga?cccattgctc????960

acagcctggc?aggaagcact?ggggtcggac?agcggcgtga?tcaacctccc?cgatgaggaa???1020

gccgaacgtc?tagcgacagc?aagcaaccat?tggcgagaag?gcgtggctgg?caacgtcgca???1080

ccagccaggg?cctgcttaga?actcttcact?cccggcgaag?gggaagacct?ctgggagctg???1140

cgcttcgcct?tacaggctga?ggctgatccc?acgatcaaag?taccggccgc?agcagcctgg???1200

gcagcgggtc?ccaaggtcct?gcaactaggc?gaaatccgtg?tggaacatcc?aggcgaggtg???1260

ctactggaag?gcatggggcg?agccctcacg?gtgtttgcac?cgatcgaacg?aggcctcgac???1320

agcgccacac?cagaagcaat?gcagctcacc?cctgctgaag?cctttgtatt?ggtgcgcact???1380

gcagcggccc?aactgcgtga?tgttggcgtt?ggcgtggaat?tgcctgccag?cctctcggga???1440

gggctggcca?gtcgcctagg?cctagcgatc?aaggcggagc?tatcggagag?atctagaggt???1500

ttcactttgg?gcgaaaccct?cgactggagt?tgggagctca?tgatcggtgg?cgtcaccctg???1560

acgcttcgcg?agctggagcg?actagcaagc?aagcgcagcc?cgcttgtcaa?ccacaagggc????1620

gcctggatcg?aattacgccc?caacgatctc?aaaaatgcgg?aacacttctg?cagcgtcaat????1680

ccaggcatca?gcctcgacga?tgccttgcgc?cttaccgcaa?ccgatggcga?cacgctgatg????1740

agactgcccg?ttcaccgctt?tgaggccggt?ccacgactac?aggcggtgtt?ggagcagtac????1800

caccagcaaa?aagctcccga?ccccctacct?gctcccgaag?gcttctgcgg?tcagctaagg????1860

ccttatcagg?aaaggggtct?gggttggctg?gccttcctgc?atcgcttcga?tcaaggggca????1920

tgcctggccg?acgacatggg?cctgggcaaa?acgatccagc?tactggcatt?cctgcaacat????1980

ctcaaggcgg?aacaggaact?caaacggccg?gtattgctta?tcgctcccac?atccgtactt????2040

accaactgga?agagagaggc?attggccttc?acaccagagt?taaacgtccg?agaacactat????2100

gggccgcgtc?ggccctctac?ccccgccgcc?ttaaagaaag?cactcaaagg?cttagacctc????2160

gttctcacca?gttacgggct?cctgcagcga?gatagtgagc?tcctggaaac?ggtcgactgg????2220

caaggagtgg?tcatcgatga?agcccaagcc?attaagaacc?ccaacgccaa?acagagccaa????2280

gcagcacgcg?atatgggccg?cccagacaaa?aacaatcgct?tcaggattgc?tcttaccggc????2340

acacccgtcg?aaaaccgagt?cagtgaactt?tgggcactga?tggacttcct?caacccaagg????2400

gttctcggtg?aagaagactt?cttccgccag?cgctaccggc?tgccaattga?acgctatggc????2460

gacatgtctt?ccctgcgaga?cctcaaaggc?cgtgttggtc?ccttcatcct?gagacgacta????2520

aaaaccgaca?aggcaatcat?ctccgaccta?cctgaaaagg?tagagctgag?cgaatgggtg????2580

ggtctgagca?aagaacaggc?agccctctat?cgcaacacag?tggatgaaac?actggaggcc????2640

attgcccgcg?cacccagtgg?tcaacgtcat?ggcaaggtgc?tcggcttgct?tacccgactg????2700

aagcaaatct?gcaaccatcc?cgccctagcc?ctcaaagaaa?aaaccgttgc?aaaaggcttc????2760

atggaccgct?ccgccaagct?gctgcgtttg?gaagaaattc?tcgaggaagt?gatcgaggca????2820

ggagatcgcg?ctctgttatt?cacccaattc?gcagaatggg?gtcatctcct?taaggcctac????2880

ctgcaacaac?gctggcgctt?tgaagttccc?ttcctgcacg?gcagcacaag?caaaactgaa????2940

cgtcaggcca?tggttgatcg?cttccaggag?gatccacgtg?gaccccaact?gttcctgctg????3000

tcactcaaag?ccggtggcgt?aggcctaaac?ctcacgcggg?ctagccatgt?gtttcatgtc????3060

gatcgctggt?ggaatcctgc?cgtagaaaac?caggccactg?atcgcgctta?caggatcgga????3120

caaaccaatc?gggtgatggt?gcacaaattc?atcaccagcg?gctcagttga?agagaaaatt????3180

gatcgcatga?ttcgcgaaaa?atctcgactt?gccgaagaca?tcattggctc?tggagaagac????3240

tggttaggtg?gcttaggcgt?cagtcaattg?cgcgaactag?tggccctaga?agacagctga????3300

<210>72

<211>1099

<212>PRT

<213>Prochlorococcus?marinus

<400>72

Met?Ile?Gly?Cys?Gly?Thr?Pro?Ala?Trp?Met?Val?Ala?Val?Asp?Arg?Gln

1???????????????5???????????????????10??????????????????15

Cys?Thr?Pro?Ala?Pro?Arg?Asn?Pro?Thr?His?Thr?Phe?Cys?Val?Ala?Ala

20??????????????????25??????????????????30

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Pro?Ala?Ile?Arg?Thr?Pro?Thr

35??????????????????40??????????????????45

Ser?Ser?Gly?Arg?Pro?Ala?Leu?Leu?Val?Trp?Ala?Asp?Thr?Trp?Arg?Val

50??????????????????55??????????????????60

Ala?Thr?Pro?Ala?Gly?Pro?Ala?Ala?Thr?Pro?Ala?Leu?His?Pro?Phe?Thr

65??????????????????70??????????????????75??????????????????80

Leu?Asn?Pro?Asp?Asp?Leu?Arg?Ala?Trp?Leu?Ile?Glu?Arg?Asp?Leu?Leu

85??????????????????90??????????????????95

Pro?Asp?Glu?Ile?Ile?Asp?Ala?Thr?Ala?Cys?Leu?Thr?Leu?Pro?Ser?Arg

100?????????????????105?????????????????110

Thr?Val?Lys?Pro?Arg?Ser?Lys?Ala?Lys?Asn?Val?Ser?Thr?Glu?Ser?Asp

115?????????????????120?????????????????125

Glu?Asp?Lys?Asp?His?Lys?Thr?Ser?Trp?Thr?Gly?Leu?Pro?Leu?Gln?Ala

130?????????????????135?????????????????140

Gly?Glu?Pro?Ile?Pro?Lys?Gln?Thr?Glu?Trp?Trp?Pro?Trp?Gln?Val?Gln

145?????????????????150?????????????????155?????????????????160

Gly?Leu?Ala?Val?Glu?Pro?Ala?Ala?Ala?Thr?Ala?Trp?Leu?Ser?Lys?Leu

165?????????????????170?????????????????175

Pro?Leu?Ser?Gly?Asp?His?Pro?Asp?Leu?Ala?Asp?Glu?Leu?Arg?Trp?Trp

180?????????????????185?????????????????190

Ser?His?Leu?Gln?Arg?Trp?Ala?Leu?Ser?Met?Ile?Ala?Arg?Gly?Arg?Trp

195?????????????????200?????????????????205

Leu?Pro?Gln?Val?Glu?Leu?Ser?Lys?Gly?Glu?Gly?Tyr?Pro?His?Arg?Ala

210?????????????????215?????????????????220

Arg?Trp?Thr?Pro?Leu?Leu?Asn?Arg?Glu?Asp?Asp?Arg?Arg?Arg?Leu?Glu

225?????????????????230?????????????????235?????????????????240

Asp?Leu?Ala?Ala?Gln?Leu?Pro?Leu?Val?Ala?Thr?Cys?Ala?Leu?Pro?Trp

245?????????????????250?????????????????255

Arg?Glu?Pro?Thr?Gly?Arg?Arg?Ser?Asn?Arg?Met?Thr?Arg?Leu?Arg?Pro

260?????????????????265?????????????????270

Glu?Ala?Met?Arg?Ala?Ala?Asn?Pro?Val?Ala?Ser?Cys?Arg?Pro?Arg?Ser

275?????????????????280?????????????????285

Gly?Arg?Leu?Arg?Val?Ala?Ser?Leu?Leu?Glu?Glu?Leu?Leu?Asp?Ala?Gln

290?????????????????295?????????????????300

Leu?Arg?Thr?Gly?Phe?Glu?Ala?Ser?Glu?Gln?Gly?Leu?Asp?Pro?Leu?Leu

305?????????????????310?????????????????315?????????????????320

Thr?Ala?Trp?Gln?Glu?Ala?Leu?Gly?Ser?Asp?Ser?Gly?Val?Ile?Asn?Leu

325?????????????????330?????????????????335

Pro?Asp?Glu?Glu?Ala?Glu?Arg?Leu?Ala?Thr?Ala?Ser?Asn?His?Trp?Arg

340?????????????????345?????????????????350

Glu?Gly?Val?Ala?Gly?Asn?Val?Ala?Pro?Ala?Arg?Ala?Cys?Leu?Glu?Leu

355?????????????????360?????????????????365

Phe?Thr?Pro?Gly?Glu?Gly?Glu?Asp?Leu?Trp?Glu?Leu?Arg?Phe?Ala?Leu

370?????????????????375?????????????????380

Gln?Ala?Glu?Ala?Asp?Pro?Thr?Ile?Lys?Val?Pro?Ala?Ala?Ala?Ala?Trp

385?????????????????390?????????????????395?????????????????400

Ala?Ala?Gly?Pro?Lys?Val?Leu?Gln?Leu?Gly?Glu?Ile?Arg?Val?Glu?His

405?????????????????410?????????????????415

Pro?Gly?Glu?Val?Leu?Leu?Glu?Gly?Met?Gly?Arg?Ala?Leu?Thr?Val?Phe

420?????????????????425?????????????????430

Ala?Pro?Ile?Glu?Arg?Gly?Leu?Asp?Ser?Ala?Thr?Pro?Glu?Ala?Met?Gln

435?????????????????440?????????????????445

Leu?Thr?Pro?Ala?Glu?Ala?Phe?Val?Leu?Val?Arg?Thr?Ala?Ala?Ala?Gln

450?????????????????455?????????????????460

Leu?Arg?Asp?Val?Gly?Val?Gly?Val?Glu?Leu?Pro?Ala?Ser?Leu?Ser?Gly

465?????????????????470?????????????????475?????????????????480

Gly?Leu?Ala?Ser?Arg?Leu?Gly?Leu?Ala?Ile?Lys?Ala?Glu?Leu?Ser?Glu

485?????????????????490?????????????????495

Arg?Ser?Arg?Gly?Phe?Thr?Leu?Gly?Glu?Thr?Leu?Asp?Trp?Ser?Trp?Glu

500?????????????????505?????????????????510

Leu?Met?Ile?Gly?Gly?Val?Thr?Leu?Thr?Leu?Arg?Glu?Leu?Glu?Arg?Leu

515?????????????????520?????????????????525

Ala?Ser?Lys?Arg?Ser?Pro?Leu?Val?Asn?His?Lys?Gly?Ala?Trp?Ile?Glu

530?????????????????535?????????????????540

Leu?Arg?Pro?Asn?Asp?Leu?Lys?Asn?Ala?Glu?His?Phe?Cys?Ser?Val?Asn

545?????????????????550?????????????????555?????????????????560

Pro?Gly?Ile?Ser?Leu?Asp?Asp?Ala?Leu?Arg?Leu?Thr?Ala?Thr?Asp?Gly

565?????????????????570?????????????????575

Asp?Thr?Leu?Met?Arg?Leu?Pro?Val?His?Arg?Phe?Glu?Ala?Gly?Pro?Arg

580?????????????????585?????????????????590

Leu?Gln?Ala?Val?Leu?Glu?Gln?Tyr?His?Gln?Gln?Lys?Ala?Pro?Asp?Pro

595?????????????????600?????????????????605

Leu?Pro?Ala?Pro?Glu?Gly?Phe?Cys?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu

610?????????????????615?????????????????620

Arg?Gly?Leu?Gly?Trp?Leu?Ala?Phe?Leu?His?Arg?Phe?Asp?Gln?Gly?Ala

625?????????????????630?????????????????635?????????????????640

Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala

645?????????????????650?????????????????655

Phe?Leu?Gln?His?Leu?Lys?Ala?Glu?Gln?Glu?Leu?Lys?Arg?Pro?Val?Leu

660?????????????????665?????????????????670

Leu?Ile?Ala?Pro?Thr?Ser?Val?Leu?Thr?Asn?Trp?Lys?Arg?Glu?Ala?Leu

675?????????????????680?????????????????685

Ala?Phe?Thr?Pro?Glu?Leu?Asn?Val?Arg?Glu?His?Tyr?Gly?Pro?Arg?Arg

690?????????????????695?????????????????700

Pro?Ser?Thr?Pro?Ala?Ala?Leu?Lys?Lys?Ala?Leu?Lys?Gly?Leu?Asp?Leu

705?????????????????710?????????????????715?????????????????720

Val?Leu?Thr?Ser?Tyr?Gly?Leu?Leu?Gln?Arg?Asp?Ser?Glu?Leu?Leu?Glu

725?????????????????730?????????????????735

Thr?Val?Asp?Trp?Gln?Gly?Val?Val?Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys

740?????????????????745?????????????????750

Asn?Pro?Asn?Ala?Lys?Gln?Ser?Gln?Ala?Ala?Arg?Asp?Met?Gly?Arg?Pro

755?????????????????760?????????????????765

Asp?Lys?Asn?Asn?Arg?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu

770?????????????????775?????????????????780

Asn?Arg?Val?Ser?Glu?Leu?Trp?Ala?Leu?Met?Asp?Phe?Leu?Asn?Pro?Arg

785?????????????????790?????????????????795?????????????????800

Val?Leu?Gly?Glu?Glu?Asp?Phe?Phe?Arg?Gln?Arg?Tyr?Arg?Leu?Pro?Ile

805?????????????????810?????????????????815

Glu?Arg?Tyr?Gly?Asp?Met?Ser?Ser?Leu?Arg?Asp?Leu?Lys?Gly?Arg?Val

820?????????????????825?????????????????830

Gly?Pro?Phe?Ile?Leu?Arg?Arg?Leu?Lys?Thr?Asp?Lys?Ala?Ile?Ile?Ser

835?????????????????840?????????????????845

Asp?Leu?Pro?Glu?Lys?Val?Glu?Leu?Ser?Glu?Trp?Val?Gly?Leu?Ser?Lys

850?????????????????855?????????????????860

Glu?Gln?Ala?Ala?Leu?Tyr?Arg?Asn?Thr?Val?Asp?Glu?Thr?Leu?Glu?Ala

865?????????????????870?????????????????875?????????????????880

Ile?Ala?Arg?Ala?Pro?Ser?Gly?Gln?Arg?His?Gly?Lys?Val?Leu?Gly?Leu

885?????????????????890?????????????????895

Leu?Thr?Arg?Leu?Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Leu?Ala?Leu?Lys

900?????????????????905?????????????????910

Glu?Lys?Thr?Val?Ala?Lys?Gly?Phe?Met?Asp?Arg?Ser?Ala?Lys?Leu?Leu

915?????????????????920?????????????????925

Arg?Leu?Glu?Glu?Ile?Leu?Glu?Glu?Val?Ile?Glu?Ala?Gly?Asp?Arg?Ala

930?????????????????935?????????????????940

Leu?Leu?Phe?Thr?Gln?Phe?Ala?Glu?Trp?Gly?His?Leu?Leu?Lys?Ala?Tyr

945?????????????????950?????????????????955?????????????????960

Leu?Gln?Gln?Arg?Trp?Arg?Phe?Glu?Val?Pro?Phe?Leu?His?Gly?Ser?Thr

965?????????????????970?????????????????975

Ser?Lys?Thr?Glu?Arg?Gln?Ala?Met?Val?Asp?Arg?Phe?Gln?Glu?Asp?Pro

980?????????????????985?????????????????990

Arg?Gly?Pro?Gln?Leu?Phe?Leu?Leu??Ser?Leu?Lys?Ala?Gly??Gly?Val?Gly

995?????????????????1000?????????????????1005

Leu?Asn??Leu?Thr?Arg?Ala?Ser??His?Val?Phe?His?Val??Asp?Arg?Trp

1010?????????????????1015?????????????????1020

Trp?Asn??Pro?Ala?Val?Glu?Asn??Gln?Ala?Thr?Asp?Arg??Ala?Tyr?Arg

1025?????????????????1030?????????????????1035

Ile?Gly??Gln?Thr?Asn?Arg?Val??Met?Val?His?Lys?Phe??Ile?Thr?Ser

1040?????????????????1045?????????????????1050

Gly?Ser??Val?Glu?Glu?Lys?Ile??Asp?Arg?Met?Ile?Arg??Glu?Lys?Ser

1055?????????????????1060?????????????????1065

Arg?Leu??Ala?Glu?Asp?Ile?Ile??Gly?Ser?Gly?Glu?Asp??Trp?Leu?Gly

1070?????????????????1075?????????????????1080

Gly?Leu??Gly?Val?Ser?Gln?Leu??Arg?Glu?Leu?Val?Ala??Leu?Glu?Asp

1085?????????????????1090?????????????????1095

Ser

<210>73

<211>3300

<212>DNA

<213>Prochlorococcus?marinus

<400>73

atgattggtt?gtggaactcc?tgcgtggatg?gttgccgttg?atcggcagtg?cactcctgct?????60

ccaagaaacc?caacacatac?tttttgcgtc?gcggccatga?gcctgctgca?cgccacctgg????120

cttccagcca?tccgtactcc?gaccagctcc?ggtcgccctg?cgctccttgt?gtgggcagat????180

acctggcgag?tcgctacccc?agcaggacca?gcagcaactc?ccgcactcca?ccccttcacc????240

ctcagcccag?acgatctacg?tgcctggctc?attgagcgcg?atctactgcc?tgatgaaatc????300

atcgacgcca?cagcatgtct?gaccctgcct?agccgaacag?tcaaaccgcg?caacaaaacc????360

aagaacgtat?ccactgaatc?cgacgaagcc?aaagacaaca?aaacaagttg?gacaggactg????420

cccttacaag?caggcgaacc?cattcccaaa?caaacagaat?ggtggccctg?gcaggtgcaa????480

ggcctggcag?tggaacctgc?tgccgcaacg?gcctggcttt?cgaaactgcc?tctttcagga????540

aatcatcctg?atctggccga?tgaattgcgc?tggtggagcc?atctacagcg?ctgggccctg????600

agcatgattg?ctcgcggacg?ttggctaccc?caggtggaac?tcagcaaggg?agagggctat????660

ccccaccgag?cacgctggac?accgctactc?aaccgtgaag?atgatcgccg?ccgcctcgaa????720

gaccttgccg?ctcagcttcc?cttagtggcc?acctgcgccc?tcccctggcg?ggagcccacc????780

ggaaggcgta?gcaaccgaat?gacccgccta?agaccagagg?cgatgcgagc?cgctaaccct????840

gtggcttcat?gccgaccccg?cagcggtcgc?cttcgcgtag?ccagcttgct?ggaagaactc????900

ttggatgccc?aactgcgcac?cggatttgaa?gcgagtgagc?aaggcctaga?cccattgctc????960

acagcctggc?aggaagcact?ggggtccgac?agcggcgtga?tcaacctccc?cgatgaggaa???1020

gccgaacgtc?tagctacagc?aagcaaccat?tggcgtgaag?gcgtggctgg?caacgtcgca???1080

ccagccagag?cctgcttaga?actcttcact?cccggagaag?gggaagacct?ctgggagctg???1140

cgcttctcct?tacaggctga?ggctgatccc?acaatcaaag?taccggccgc?agcagcctgg???1200

gcagctggtc?ccaaggtgtt?gcaactaggc?gaaatccgtg?tggaacatcc?aggcgaggtg???1260

ctactggaag?gcatggggcg?agccctcacg?gtgtttgcac?cgatcgaacg?aggcctcgac???1320

agcgccacac?cagaagcaat?gcagctcacc?cctgctgaag?cctttgtatt?ggtgcgcact????1380

gcagcgaccc?aactgcgtga?tgttggcgtt?ggcgtggaat?tgcctgccag?cctctcggga????1440

gggctggcca?gtcgcctagg?cctagcgatc?aaggcggagc?tatcggagag?atctagaggt????1500

ttcactctgg?gcgaaaccct?cgactggagt?tgggagctca?tgatcggtgg?cgtcaccctg????1560

acgcttcgcg?aactggagcg?actagcaagc?aagcgcagcc?cgcttgtcaa?ccacaagggc????1620

gcctggatcg?aattacgccc?caacgatctc?aaacatgcgg?aacacttctg?cagcgtcaat????1680

ccaggcatca?gcctcgacga?tgccttgcgc?cttaccgcaa?cagatggcga?cacgctgatg????1740

agactgcccg?ttcaccgctt?tgaggccggt?ccacgactac?aggcggtgtt?ggagcagtac????1800

caccagcaaa?aagcaccaga?ccccctacct?gctcccgaag?gcttctgcgg?tcagctaagg????1860

ccttatcagg?aaaggggtct?gggttggctg?gccttcctgc?atcgcttcga?tcaaggggca????1920

tgcctggccg?acgacatggg?ccttggcaaa?acgatccagc?tactggcatt?cctgcaacat????1980

ctcaaggcgg?aacaggaact?caaacggccg?gtattgctta?tcgctcccac?gtccgtactc????2040

accaactgga?agagagaggc?gttggccttc?acaccagagt?taaacgtccg?cgaacactat????2100

gggccgcgtc?ggccctctac?ccccgccgcc?ttaaagaaag?cactcaaagg?cttagacctc????2160

gttctcacca?gttatgggct?cctgcagcga?gatagtgagc?tcctggaaac?ggtcgactgg????2220

caaggcgtgg?tcatcgatga?agcccaagcc?attaagaacc?ccaacgccaa?acagagccaa????2280

gcagcacgcg?atatgggccg?cccagacaaa?aacaatcgct?tcaggattgc?tcttaccggc????2340

acacccgtcg?aaaaccgagt?aagtgaactt?tgggcactaa?tggacttcct?taacccaagg????2400

gttctcggtg?aagaagactt?cttccgccag?cgctaccggc?tgccgattga?gcgctatggc????2460

gacatgtctt?ccctgcgaga?cctcaagggc?cgtgttggtc?ccttcatcct?gagacgactc????2520

aaaaccgaca?aggcaatcat?ctccgaccta?cccgaaaaag?tagagctgag?cgaatgggtg????2580

gggctgagca?aagaacaggc?agccctctat?cgcaacacag?tggatgaaac?actggaggcc????2640

attgcccgcg?cacccagggg?tcaacgccat?ggcaaggtgc?tcggattgct?taccagactg????2700

aagcaaatct?gcaaccatcc?cgccctagcc?ctcaaagaac?aaaccgttgc?aaaagggttc????2760

atggaccgct?ccgccaagct?gctgcgtttg?gaagaaattc?tcgaagaagt?aatcgaggca????2820

ggagatcgcg?ctctgttatt?cacccaattc?gcagaatggg?gtcatctcct?taaggcctac????2880

ctgcaacaac?gctggcgctt?tgaagttccc?ttcctgcacg?gcagcacaag?caaaactgaa????2940

cgtcaggcca?tggttgatcg?cttccaggag?gatccacgtg?gaccccaact?gttcctgctg????3000

tcactcaaag?ccggtggtgt?aggcctcaac?ctgacgcggg?ctagccatgt?gtttcatgtt????3060

gatcgctggt?ggaatcctgc?cgtagaaaac?caggccactg?atcgcgctta?caggatcggg????3120

caaaccagtc?gggtgatggt?gcacaaattc?atcaccagcg?gctcagttga?agagaaaatt????3180

gatcgcatga?ttcgtgaaaa?atctcgactt?gccgaagaca?tcattggctc?tggagaagac????3240

tggttaggtg?gcttaggcgt?cagtcaattg?cgcgaactag?tggccctaga?agacagctga????3300

<210>74

<211>1099

<212>PRT

<213>Prochlorococcus?marinus

<400>74

Met?Ile?Gly?Cys?Gly?Thr?Pro?Ala?Trp?Met?Val?Ala?Val?Asp?Arg?Gln

1???????????????5???????????????????10??????????????????15

Cys?Thr?Pro?Ala?Pro?Arg?Asn?Pro?Thr?His?Thr?Phe?Cys?Val?Ala?Ala

20??????????????????25??????????????????30

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Pro?Ala?Ile?Arg?Thr?Pro?Thr

35??????????????????40??????????????????45

Ser?Ser?Gly?Arg?Pro?Ala?Leu?Leu?Val?Trp?Ala?Asp?Thr?Trp?Arg?Val

50??????????????????55??????????????????60

Ala?Thr?Pro?Ala?Gly?Pro?Ala?Ala?Thr?Pro?Ala?Leu?His?Pro?Phe?Thr

65??????????????????70??????????????????75??????????????????80

Leu?Ser?Pro?Asp?Asp?Leu?Arg?Ala?Trp?Leu?Ile?Glu?Arg?Asp?Leu?Leu

85??????????????????90??????????????????95

Pro?Asp?Glu?Ile?Ile?Asp?Ala?Thr?Ala?Cys?Leu?Thr?Leu?Pro?Ser?Arg

100?????????????????105?????????????????110

Thr?Val?Lys?Pro?Arg?Asn?Lys?Thr?Lys?Asn?Val?Ser?Thr?Glu?Ser?Asp

115?????????????????120?????????????????125

Glu?Ala?Lys?Asp?Asn?Lys?Thr?Ser?Trp?Thr?Gly?Leu?Pro?Leu?Gln?Ala

130?????????????????135?????????????????140

Gly?Glu?Pro?Ile?Pro?Lys?Gln?Thr?Glu?Trp?Trp?Pro?Trp?Gln?Val?Gln

145?????????????????150?????????????????155?????????????????160

Gly?Leu?Ala?Val?Glu?Pro?Ala?Ala?Ala?Thr?Ala?Trp?Leu?Ser?Lys?Leu

165?????????????????170?????????????????175

Pro?Leu?Ser?Gly?Asn?His?Pro?Asp?Leu?Ala?Asp?Glu?Leu?Arg?Trp?Trp

180?????????????????185?????????????????190

Ser?His?Leu?Gln?Arg?Trp?Ala?Leu?Ser?Met?Ile?Ala?Arg?Gly?Arg?Trp

195?????????????????200?????????????????205

Leu?Pro?Gln?Val?Glu?Leu?Ser?Lys?Gly?Glu?Gly?Tyr?Pro?His?Arg?Ala

210?????????????????215?????????????????220

Arg?Trp?Thr?Pro?Leu?Leu?Asn?Arg?Glu?Asp?Asp?Arg?Arg?Arg?Leu?Glu

225?????????????????230?????????????????235?????????????????240

Asp?Leu?Ala?Ala?Gln?Leu?Pro?Leu?Val?Ala?Thr?Cys?Ala?Leu?Pro?Trp

245?????????????????250?????????????????255

Arg?Glu?Pro?Thr?Gly?Arg?Arg?Ser?Asn?Arg?Met?Thr?Arg?Leu?Arg?Pro

260?????????????????265?????????????????270

Glu?Ala?Met?Arg?Ala?Ala?Asn?Pro?Val?Ala?Ser?Cys?Arg?Pro?Arg?Ser

275?????????????????280?????????????????285

Gly?Arg?Leu?Arg?Val?Ala?Ser?Leu?Leu?Glu?Glu?Leu?Leu?Asp?Ala?Gln

290?????????????????295?????????????????300

Leu?Arg?Thr?Gly?Phe?Glu?Ala?Ser?Glu?Gln?Gly?Leu?Asp?Pro?Leu?Leu

305?????????????????310?????????????????315?????????????????320

Thr?Ala?Trp?Gln?Glu?Ala?Leu?Gly?Ser?Asp?Ser?Gly?Val?Ile?Asn?Leu

325?????????????????330?????????????????335

Pro?Asp?Glu?Glu?Ala?Glu?Arg?Leu?Ala?Thr?Ala?Ser?Asn?His?Trp?Arg

340?????????????????345?????????????????350

Glu?Gly?Val?Ala?Gly?Asn?Val?Ala?Pro?Ala?Arg?Ala?Cys?Leu?Glu?Leu

355?????????????????360?????????????????365

Phe?Thr?Pro?Gly?Glu?Gly?Glu?Asp?Leu?Trp?Glu?Leu?Arg?Phe?Ser?Leu

370?????????????????375?????????????????380

Gln?Ala?Glu?Ala?Asp?Pro?Thr?Ile?Lys?Val?Pro?Ala?Ala?Ala?Ala?Trp

385?????????????????390?????????????????395?????????????????400

Ala?Ala?Gly?Pro?Lys?Val?Leu?Gln?Leu?Gly?Glu?Ile?Arg?Val?Glu?His

405?????????????????410?????????????????415

Pro?Gly?Glu?Val?Leu?Leu?Glu?Gly?Met?Gly?Arg?Ala?Leu?Thr?Val?Phe

420?????????????????425?????????????????430

Ala?Pro?Ile?Glu?Arg?Gly?Leu?Asp?Ser?Ala?Thr?Pro?Glu?Ala?Met?Gln

435?????????????????440?????????????????445

Leu?Thr?Pro?Ala?Glu?Ala?Phe?Val?Leu?Val?Arg?Thr?Ala?Ala?Thr?Gln

450?????????????????455?????????????????460

Leu?Arg?Asp?Val?Gly?Val?Gly?Val?Glu?Leu?Pro?Ala?Ser?Leu?Ser?Gly

465?????????????????470?????????????????475?????????????????480

Gly?Leu?Ala?Ser?Arg?Leu?Gly?Leu?Ala?Ile?Lys?Ala?Glu?Leu?Ser?Glu

485?????????????????490?????????????????495

Arg?Ser?Arg?Gly?Phe?Thr?Leu?Gly?Glu?Thr?Leu?Asp?Trp?Ser?Trp?Glu

500?????????????????505?????????????????510

Leu?Met?Ile?Gly?Gly?Val?Thr?Leu?Thr?Leu?Arg?Glu?Leu?Glu?Arg?Leu

515?????????????????520?????????????????525

Ala?Ser?Lys?Arg?Ser?Pro?Leu?Val?Asn?His?Lys?Gly?Ala?Trp?Ile?Glu

530?????????????????535?????????????????540

Leu?Arg?Pro?Asn?Asp?Leu?Lys?His?Ala?Glu?His?Phe?Cys?Ser?Val?Asn

545?????????????????550?????????????????555?????????????????560

Pro?Gly?Ile?Ser?Leu?Asp?Asp?Ala?Leu?Arg?Leu?Thr?Ala?Thr?Asp?Gly

565?????????????????570?????????????????575

Asp?Thr?Leu?Met?Arg?Leu?Pro?Val?His?Arg?Phe?Glu?Ala?Gly?Pro?Arg

580?????????????????585?????????????????590

Leu?Gln?Ala?Val?Leu?Glu?Gln?Tyr?His?Gln?Gln?Lys?Ala?Pro?Asp?Pro

595?????????????????600?????????????????605

Leu?Pro?Ala?Pro?Glu?Gly?Phe?Cys?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu

610?????????????????615?????????????????620

Arg?Gly?Leu?Gly?Trp?Leu?Ala?Phe?Leu?His?Arg?Phe?Asp?Gln?Gly?Ala

625?????????????????630?????????????????635?????????????????640

Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala

645?????????????????650?????????????????655

Phe?Leu?Gln?His?Leu?Lys?Ala?Glu?Gln?Glu?Leu?Lys?Arg?Pro?Val?Leu

660?????????????????665?????????????????670

Leu?Ile?Ala?Pro?Thr?Ser?Val?Leu?Thr?Asn?Trp?Lys?Arg?Glu?Ala?Leu

675?????????????????680?????????????????685

Ala?Phe?Thr?Pro?Glu?Leu?Asn?Val?Arg?Glu?His?Tyr?Gly?Pro?Arg?Arg

690?????????????????695?????????????????700

Pro?Ser?Thr?Pro?Ala?Ala?Leu?Lys?Lys?Ala?Leu?Lys?Gly?Leu?Asp?Leu

705?????????????????710?????????????????715?????????????????720

Val?Leu?Thr?Ser?Tyr?Gly?Leu?Leu?Gln?Arg?Asp?Ser?Glu?Leu?Leu?Glu

725?????????????????730?????????????????735

Thr?Val?Asp?Trp?Gln?Gly?Val?Val?Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys

740?????????????????745?????????????????750

Asn?Pro?Asn?Ala?Lys?Gln?Ser?Gln?Ala?Ala?Arg?Asp?Met?Gly?Arg?Pro

755?????????????????760?????????????????765

Asp?Lys?Asn?Asn?Arg?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu

770?????????????????775?????????????????780

Asn?Arg?Val?Ser?Glu?Leu?Trp?Ala?Leu?Met?Asp?Phe?Leu?Asn?Pro?Arg

785?????????????????790?????????????????795?????????????????800

Val?Leu?Gly?Glu?Glu?Asp?Phe?Phe?Arg?Gln?Arg?Tyr?Arg?Leu?Pro?Ile

805?????????????????810?????????????????815

Glu?Arg?Tyr?Gly?Asp?Met?Ser?Ser?Leu?Arg?Asp?Leu?Lys?Gly?Arg?Val

820?????????????????825?????????????????830

Gly?Pro?Phe?Ile?Leu?Arg?Arg?Leu?Lys?Thr?Asp?Lys?Ala?Ile?Ile?Ser

835?????????????????840?????????????????845

Asp?Leu?Pro?Glu?Lys?Val?Glu?Leu?Ser?Glu?Trp?Val?Gly?Leu?Ser?Lys

850?????????????????855?????????????????860

Glu?Gln?Ala?Ala?Leu?Tyr?Arg?Asn?Thr?Val?Asp?Glu?Thr?Leu?Glu?Ala

865?????????????????870?????????????????875?????????????????880

Ile?Ala?Arg?Ala?Pro?Arg?Gly?Gln?Arg?His?Gly?Lys?Val?Leu?Gly?Leu

885?????????????????890?????????????????895

Leu?Thr?Arg?Leu?Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Leu?Ala?Leu?Lys

900?????????????????905?????????????????910

Glu?Gln?Thr?Val?Ala?Lys?Gly?Phe?Met?Asp?Arg?Ser?Ala?Lys?Leu?Leu

915?????????????????920?????????????????925

Arg?Leu?Glu?Glu?Ile?Leu?Glu?Glu?Val?Ile?Glu?Ala?Gly?Asp?Arg?Ala

930?????????????????935?????????????????940

Leu?Leu?Phe?Thr?Gln?Phe?Ala?Glu?Trp?Gly?His?Leu?Leu?Lys?Ala?Tyr

945?????????????????950?????????????????955?????????????????960

Leu?Gln?Gln?Arg?Trp?Arg?Phe?Glu?Val?Pro?Phe?Leu?His?Gly?Ser?Thr

965?????????????????970?????????????????975

Ser?Lys?Thr?Glu?Arg?Gln?Ala?Met?Val?Asp?Arg?Phe?Gln?Glu?Asp?Pro

980?????????????????985?????????????????990

Arg?Gly?Pro?Gln?Leu?Phe?Leu?Leu??Ser?Leu?Lys?Ala?Gly??Gly?Val?Gly

995?????????????????1000?????????????????1005

Leu?Asn??Leu?Thr?Arg?Ala?Ser??His?Val?Phe?His?Val??Asp?Arg?Trp

1010?????????????????1015?????????????????1020

Trp?Asn??Pro?Ala?Val?Glu?Asn??Gln?Ala?Thr?Asp?Arg??Ala?Tyr?Arg

1025?????????????????1030?????????????????1035

Ile?Gly??Gln?Thr?Ser?Arg?Val??Met?Val?His?Lys?Phe??Ile?Thr?Ser

1040?????????????????1045?????????????????1050

Gly?Ser??Val?Glu?Glu?Lys?Ile??Asp?Arg?Met?Ile?Arg??Glu?Lys?Ser

1055?????????????????1060?????????????????1065

Arg?Leu??Ala?Glu?Asp?Ile?Ile??Gly?Ser?Gly?Glu?Asp??Trp?Leu?Gly

1070?????????????????1075?????????????????1080

Gly?Leu??Gly?Val??Ser?Gln?Leu??Arg?Glu?Leu?Val?Ala??Leu?Glu?Asp

1085?????????????????1090?????????????????1095

Ser

<210>75

<211>2886

<212>DNA

＜213〉rhodococcus

<400>75

atggcgcgag?cagggacttc?acgcgctgtc?ggtcgcacct?gcttggatgg?gtgcatgctg?????60

cacggcctct?ggacaccggg?ttcgggtctc?atgctgtggg?tggaggatcg?gaatccggca????120

gctccggagc?cgacggacgc?ggtcgggcgg?atgctggcgc?ggaagttccg?gcatcacgtg????180

aaggtgccga?tgccgacgcc?gtcggggccg?gagatgctcg?agtgggccgc?ggttgcgctc????240

gcaccaccgg?atgcgacgga?gttcctgctg?tcggtgtcgt?cccgcgaccc?ccggatcgcc????300

ggggatctgc?gctacctcgc?ccacgtcgcc?cgcggtgtcg?agcggtgggc?acgggccggg????360

cgggtggtgc?ccgaggtaca?ccgggcggag?ggcggctggt?ggccgcgctg?gcggctgctc????420

ggcggtgaac?ggcagcgtgc?gtggctcacg?gagctggccg?tggcgatgcc?gccggtccag????480

cgtcacggca?cgaccccccg?ggccgtgctc?gacgacatgg?tcaccgagct?gaccgacccc????540

gtcgcccgcc?gtgtcctcga?acgacggcac?ccggacgatt?ccggcggcga?cgtggatcat????600

ccgctgatcg?acgcgctcgt?gcggggtgac?cagttcgccg?agggcaccgc?ccagctgtcg????660

ggatcgctgg?acgggtggcg?cgacagcctc?aaggtggacg?agcccgaact?ggtgctgcgg????720

ctcctcgagc?cggaagacgt?ggacgtggag?ggggattggg?acccggacac?ggtgctgtgg????780

cgactggagg?tctgccttcg?accggaaggc?gaagccccgg?tgccgattcc?gttgcaccgc????840

acggaggcga?gtcgtctgca?gatcggggtg?cgcaagctga?cggaggccgt?ggccgcctac????900

ccgcgactgc?aggacgttcc?cagtgacccc?gacagcctgg?acctgatgtt?gcccaccgcc????960

gtggtcatcg?accttgtcgg?gcacggtgcg?gtggcgttga?aggagaaggg?catcagcctg???1020

ctgctgccgc?gggcgtggag?tgtggcgtcg?ccgtcgatgc?gtctgcgggt?gagctcgccg???1080

agcactccgg?cgagcgcgga?gaaccgggcc?gtcggcaaag?accagttggt?gcaatacaac????1140

tgggagctgg?cactcggcga?cacggtgctc?accgccgcgg?agatgaatcg?actggtcaac????1200

tccaagagcg?atctcgtgcg?gttgcgcggt?gagtgggttc?gggcggatca?ggaggtgctc????1260

tcccgcgccg?cgcgctacgt?ggcggagcgg?cacgccagcg?gcgaccgggc?catcgtggac????1320

ctgctgaagg?acctgatcgc?ggacgatctg?tccgatcttc?ccgtggagga?ggtcacggcc????1380

accggctggg?cggccgcgtt?gctggacggc?gacacgaagc?cgcaggacgt?gccgaccccg????1440

gacgggttgg?acgccacgct?gcgcccgtac?cagaagcggg?ggctcgactg?gctggtgttc????1500

atgagccgtc?tcggcctcgg?ggccgtcctc?gccgacgaca?tgggactcgg?caagacgctg????1560

cagttgctgg?cgctgctggc?acacgagaag?gcgcccacgc?ccacgctgct?ggtgtgcccg????1620

atgtcggtgg?tcggcaactg?gcagcgcgag?gcagcgcgct?tcgtcccctc?gctgcgggtg????1680

ctcgtccacc?acggtccgca?gcggctgagc?ggcgcggagt?tcaccgccgc?cgtgacacag????1740

agcgatctgg?tgatcaccac?gtatgcgctg?ctggcccgcg?acgtcgcgca?cctgaaggag????1800

caggactggc?ggcgtgtcgt?gctggacgag?gcgcagcaca?tcaagaacgc?gaagacgtcg????1860

caggcgcggg?cggcgcggag?cattccggcg?gcgcaccgcg?tcgcgctgac?cggcactccg????1920

gtcgagaacc?gcctcgacga?actgcgctcg?atcctcgact?tcgcgaactc?gggcatcctg????1980

ggctcggagg?tgatgttccg?caagcgcttc?gtggtgccga?tcgagcggga?gcaggacgag????2040

acagccgtcg?cccggctccg?cgcggtcacg?tccccgttcg?tgctgcgccg?ggtcaagacc????2100

gatcccgcgg?tcatcgccga?cctccccgac?aagttcgaga?tgacggtgcg?cgccaacctc????2160

accgcggagc?aggccgcgct?gtaccgggcg?gtggtcgacg?acatgatggc?gcagatcaag????2220

gacaagaagg?ggatgaagcg?caagggcgcc?gtcctcgccg?ccctgacgaa?actcaagcag????2280

gtgtgcaacc?acccggcaca?cttcctgcgc?gacgggtcgg?cggtgatgcg?gcgcggacag????2340

caccgctccg?gcaagctggg?gctcgtcgag?gacatcctgg?attccgtggt?cgcggacggc????2400

gagaaggcgt?tgctgttcac?ccagttccgg?gaattcggcg?acctcgtcac?cccgtacctc??2460

gcggagcgtt?tcggtactcc?cgtgccgttt?ctgcacgggg?gcgtgtccaa?gcagaagcgc??2520

gacgacatgg?tggcctcgtt?ccagggcgac?gacgggccgc?cgatcatgat?gctctcgctg??2580

aaggcgggcg?ggacgggttt?gaacctcacc?gcggccaatc?acgtcgtcca?cctcgaccgg??2640

tggtggaatc?cggcggtcga?gaaccaggcc?acggacaggg?cgttccggat?cggccagcgg??2700

cgggacgtgc?aggtgcgcaa?gctcgtgtgc?gtcggcaccc?tggaggagcg?gatcgacgcg??2760

atgatcgcca?ccaagcagga?gctggccgat?ctcgccgtcg?ggacgggcga?gaactgggtg??2820

acggagatga?gcaccgaaca?actgggcgaa?ctgctccgcc?tcggtgacga?ggcggtgggc??2880

gaatga?????????????????????????????????????????????????????????????2886

<210>76

<211>961

<212>PRT

＜213〉rhodococcus

<400>76

Met?Ala?Arg?Ala?Gly?Thr?Ser?Arg?Ala?Val?Gly?Arg?Thr?Cys?Leu?Asp

1???????????????5???????????????????10??????????????????15

Gly?Cys?Met?Leu?His?Gly?Leu?Trp?Thr?Pro?Gly?Ser?Gly?Leu?Met?Leu

20??????????????????25??????????????????30

Trp?Val?Glu?Asp?Arg?Asn?Pro?Ala?Ala?Pro?Glu?Pro?Thr?Asp?Ala?Val

35??????????????????40??????????????????45

Gly?Arg?Met?Leu?Ala?Arg?Lys?Phe?Arg?His?His?Val?Lys?Val?Pro?Met

50??????????????????55??????????????????60

Pro?Thr?Pro?Ser?Gly?Pro?Glu?Met?Leu?Glu?Trp?Ala?Ala?Val?Ala?Leu

65??????????????????70??????????????????75??????????????????80

Ala?Pro?Pro?Asp?Ala?Thr?Glu?Phe?Leu?Leu?Ser?Val?Ser?Ser?Arg?Asp

85??????????????????90??????????????????95

Pro?Arg?Ile?Ala?Gly?Asp?Leu?Arg?Tyr?Leu?Ala?His?Val?Ala?Arg?Gly

100?????????????????105?????????????????110

Val?Glu?Arg?Trp?Ala?Arg?Ala?Gly?Arg?Val?Val?Pro?Glu?Val?His?Arg

115?????????????????120?????????????????125

Ala?Glu?Gly?Gly?Trp?Trp?Pro?Arg?Trp?Arg?Leu?Leu?Gly?Gly?Glu?Arg

130?????????????????135?????????????????140

Gln?Arg?Ala?Trp?Leu?Thr?Glu?Leu?Ala?Val?Ala?Met?Pro?Pro?Val?Gln

145?????????????????150?????????????????155?????????????????160

Arg?His?Gly?Thr?Thr?Pro?Arg?Ala?Val?Leu?Asp?Asp?Met?Val?Thr?Glu

165?????????????????170?????????????????175

Leu?Thr?Asp?Pro?Val?Ala?Arg?Arg?Val?Leu?Glu?Arg?Arg?His?Pro?Asp

180?????????????????185?????????????????190

Asp?Ser?Gly?Gly?Asp?Val?Asp?His?Pro?Leu?Ile?Asp?Ala?Leu?Val?Arg

195?????????????????200?????????????????205

Gly?Asp?Gln?Phe?Ala?Glu?Gly?Thr?Ala?Gln?Leu?Ser?Gly?Ser?Leu?Asp

210?????????????????215?????????????????220

Gly?Trp?Arg?Asp?Ser?Leu?Lys?Val?Asp?Glu?Pro?Glu?Leu?Val?Leu?Arg

225?????????????????230?????????????????235?????????????????240

Leu?Leu?Glu?Pro?Glu?Asp?Val?Asp?Val?Glu?Gly?Asp?Trp?Asp?Pro?Asp

245?????????????????250?????????????????255

Thr?Val?Leu?Trp?Arg?Leu?Glu?Val?Cys?Leu?Arg?Pro?Glu?Gly?Glu?Ala

260?????????????????265?????????????????270

Pro?Val?Pro?Ile?Pro?Leu?His?Arg?Thr?Glu?Ala?Ser?Arg?Leu?Gln?Ile

275?????????????????280?????????????????285

Gly?Val?Arg?Lys?Leu?Thr?Glu?Ala?Val?Ala?Ala?Tyr?Pro?Arg?Leu?Gln

290?????????????????295?????????????????300

Asp?Val?Pro?Ser?Asp?Pro?Asp?Ser?Leu?Asp?Leu?Met?Leu?Pro?Thr?Ala

305?????????????????310?????????????????315?????????????????320

Val?Val?Ile?Asp?Leu?Val?Gly?His?Gly?Ala?Val?Ala?Leu?Lys?Glu?Lys

325?????????????????330?????????????????335

Gly?Ile?Ser?Leu?Leu?Leu?Pro?Arg?Ala?Trp?Ser?Val?Ala?Ser?Pro?Ser

340?????????????????345?????????????????350

Met?Arg?Leu?Arg?Val?Ser?Ser?Pro?Ser?Thr?Pro?Ala?Ser?Ala?Glu?Asn

355?????????????????360?????????????????365

Arg?Ala?Val?Gly?Lys?Asp?Gln?Leu?Val?Gln?Tyr?Asn?Trp?Glu?Leu?Ala

370?????????????????375?????????????????380

Leu?Gly?Asp?Thr?Val?Leu?Thr?Ala?Ala?Glu?Met?Asn?Arg?Leu?Val?Asn

385?????????????????390?????????????????395?????????????????400

Ser?Lys?Ser?Asp?Leu?Val?Arg?Leu?Arg?Gly?Glu?Trp?Val?Arg?Ala?Asp

405?????????????????410?????????????????415

Gln?Glu?Val?Leu?Ser?Arg?Ala?Ala?Arg?Tyr?Val?Ala?Glu?Arg?His?Ala

420?????????????????425?????????????????430

Ser?Gly?Asp?Arg?Ala?Ile?Val?Asp?Leu?Leu?Lys?Asp?Leu?Ile?Ala?Asp

435?????????????????440?????????????????445

Asp?Leu?Ser?Asp?Leu?Pro?Val?Glu?Glu?Val?Thr?Ala?Thr?Gly?Trp?Ala

450?????????????????455?????????????????460

Ala?Ala?Leu?Leu?Asp?Gly?Asp?Thr?Lys?Pro?Gln?Asp?Val?Pro?Thr?Pro

465?????????????????470?????????????????475?????????????????480

Asp?Gly?Leu?Asp?Ala?Thr?Leu?Arg?Pro?Tyr?Gln?Lys?Arg?Gly?Leu?Asp

485?????????????????490?????????????????495

Trp?Leu?Val?Phe?Met?Ser?Arg?Leu?Gly?Leu?Gly?Ala?Val?Leu?Ala?Asp

500?????????????????505?????????????????510

Asp?Met?Gly?Leu?Gly?Lys?Thr?Leu?Gln?Leu?Leu?Ala?Leu?Leu?Ala?His

515?????????????????520?????????????????525

Glu?Lys?Ala?Pro?Thr?Pro?Thr?Leu?Leu?Val?Cys?Pro?Met?Ser?Val?Val

530?????????????????535?????????????????540

Gly?Asn?Trp?Gln?Arg?Glu?Ala?Ala?Arg?Phe?Val?Pro?Ser?Leu?Arg?Val

545?????????????????550?????????????????555?????????????????560

Leu?Val?His?His?Gly?Pro?Gln?Arg?Leu?Ser?Gly?Ala?Glu?Phe?Thr?Ala

565?????????????????570?????????????????575

Ala?Val?Thr?Gln?Ser?Asp?Leu?Val?Ile?Thr?Thr?Tyr?Ala?Leu?Leu?Ala

580?????????????????585?????????????????590

Arg?Asp?Val?Ala?His?Leu?Lys?Glu?Gln?Asp?Trp?Arg?Arg?Val?Val?Leu

595?????????????????600?????????????????605

Asp?Glu?Ala?Gln?His?Ile?Lys?Asn?Ala?Lys?Thr?Ser?Gln?Ala?Arg?Ala

610?????????????????615?????????????????620

Ala?Arg?Ser?Ile?Pro?Ala?Ala?His?Arg?Val?Ala?Leu?Thr?Gly?Thr?Pro

625?????????????????630?????????????????635?????????????????640

Val?Glu?Asn?Arg?Leu?Asp?Glu?Leu?Arg?Ser?Ile?Leu?Asp?Phe?Ala?Asn

645?????????????????650?????????????????655

Ser?Gly?Ile?Leu?Gly?Ser?Glu?Val?Met?Phe?Arg?Lys?Arg?Phe?Val?Val

660?????????????????665?????????????????670

Pro?Ile?Glu?Arg?Glu?Gln?Asp?Glu?Thr?Ala?Val?Ala?Arg?Leu?Arg?Ala

675?????????????????680?????????????????685

Val?Thr?Ser?Pro?Phe?Val?Leu?Arg?Arg?Val?Lys?Thr?Asp?Pro?Ala?Val

690?????????????????695?????????????????700

Ile?Ala?Asp?Leu?Pro?Asp?Lys?Phe?Glu?Met?Thr?Val?Arg?Ala?Asn?Leu

705?????????????????710?????????????????715?????????????????720

Thr?Ala?Glu?Gln?Ala?Ala?Leu?Tyr?Arg?Ala?Val?Val?Asp?Asp?Met?Met

725?????????????????730?????????????????735

Ala?Gln?Ile?Lys?Asp?Lys?Lys?Gly?Met?Lys?Arg?Lys?Gly?Ala?Val?Leu

740?????????????????745?????????????????750

Ala?Ala?Leu?Thr?Lys?Leu?Lys?Gln?Val?Cys?Asn?His?Pro?Ala?His?Phe

755?????????????????760?????????????????765

Leu?Arg?Asp?Gly?Ser?Ala?Val?Met?Arg?Arg?Gly?Gln?His?Arg?Ser?Gly

770?????????????????775?????????????????780

Lys?Leu?Gly?Leu?Val?Glu?Asp?Ile?Leu?Asp?Ser?Val?Val?Ala?Asp?Gly

785?????????????????790?????????????????795?????????????????800

Glu?Lys?Ala?Leu?Leu?Phe?Thr?Gln?Phe?Arg?Glu?Phe?Gly?Asp?Leu?Val

805?????????????????810?????????????????815

Thr?Pro?Tyr?Leu?Ala?Glu?Arg?Phe?Gly?Thr?Pro?Val?Pro?Phe?Leu?His

820?????????????????825?????????????????830

Gly?Gly?Val?Ser?Lys?Gln?Lys?Arg?Asp?Asp?Met?Val?Ala?Ser?Phe?Gln

835?????????????????840?????????????????845

Gly?Asp?Asp?Gly?Pro?Pro?Ile?Met?Met?Leu?Ser?Leu?Lys?Ala?Gly?Gly

850?????????????????855?????????????????860

Thr?Gly?Leu?Asn?Leu?Thr?Ala?Ala?Asn?His?Val?Val?His?Leu?Asp?Arg

865?????????????????870?????????????????875?????????????????880

Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp?Arg?Ala?Phe?Arg

885?????????????????890?????????????????895

Ile?Gly?Gln?Arg?Arg?Asp?Val?Gln?Val?Arg?Lys?Leu?Val?Cys?Val?Gly

900?????????????????905?????????????????910

Thr?Leu?Glu?Glu?Arg?Ile?Asp?Ala?Met?Ile?Ala?Thr?Lys?Gln?Glu?Leu

915?????????????????920?????????????????925

Ala?Asp?Leu?Ala?Val?Gly?Thr?Gly?Glu?Asn?Trp?Val?Thr?Glu?Met?Ser

930?????????????????935?????????????????940

Thr?Glu?Gln?Leu?Gly?Glu?Leu?Leu?Arg?Leu?Gly?Asp?Glu?Ala?Val?Gly

945?????????????????950?????????????????955?????????????????960

Glu

<210>77

<211>3153

<212>DNA

<213>Salinispora?tropica

<400>77

gtgctggttg?tccacgggtc?gtggcggctc?ggcatcgggc?tcgccatctg?ggccgaggac?????60

agcgcgtcgc?cgcctcgggc?gccgcgccgg?gccgggcggg?cgccccgcga?gcgaccccac????120

ccgttcgccg?ccggtcaccc?cgtgcttgcg?gcagctctgg?ccgaggtcgc?cgagccgacc????180

gagcccggca?cggcactgct?caccctgccc?acccgagctg?gttcgccgct?ggactcgccg????240

gagctggtcc?gcaccgcgtc?ggtcgagccg?ctccgtgggc?cggtcacgtt?ggccgggtgg????300

cgggtgcccg?ccctggttta?cgccccggac?gccgccctgt?cgctgctctc?ccagatcacc????360

gcggccggcg?ctctacctga?cgccgtaccc?ggtgccactc?tgcgtcacct?cgcggagctg????420

gcggccttcg?ccgtggacct?cgccgcccgt?ggtcgggtcc?tgcccggcgt?ccggccaccg????480

aaggaacgtg?ccagcgccgc?ctgggcggtg?tggcagcccc?tgctcaccgg?cgtggacgct????540

ggctgggccc?gggccctcgc?cctcgccctg?ccgcccgcgg?tccgtgccgc?cgtcgagatc????600

gatccggctc?cactcgccgt?acccggcgga?ccggaaacgc?ccgccaacgg?tggtgtgccg????660

ccgcaggctc?gtacgaggcg?accgaccgca?gccgccgggg?aaccaggtga?actggtggtc????720

gaggcgctcg?acgcgctcac?cgacgcggcc?gtacgggctg?ccctcgcgga?gacctccctt????780

acccggggag?cccgtccgcg?gggcgcggtc?gcggcctggc?tcgcggcgct?caccggcccg????840

cgtcgtgact?tcaccgccga?ctcggcggag?ctcgacaccc?tgcgcggtga?gttggacgcc????900

tggcagcgcg?acgctgtggg?aggttcggtc?cgggccagct?tccggctggt?ggagccgccg????960

acggacggac?tctttgaggc?ggcggccggg?gggctggccg?cggccgaggg?gtcgtggcgg????1020

gtcgagttcg?gcctacagcc?ggccgaccag?ccgggtctgc?atgttgacgc?cgtgcggatc????1080

tggcacgagt?cggcggccct?accgggcccg?gccgctccgc?aggaggccct?gctgaccgag????1140

ttggggcggg?ccagccgact?ctggccggag?ctgaactcgg?ccctgcgcac?cgccactcca????1200

gaggcgctgg?agctggacgc?cgcgggcgcg?catcgctttc?tacgcgacgg?cgcgccggtg????1260

ctgcacgcag?ccgggttcgc?ggtgctgttg?ccctcgtggt?ggcagcgtcc?gtcgtcccgg????1320

ctcggcgctc?gactacaggc?ccagagccgt?accgccccgg?gcaccgtcgc?cggggctggc????1380

gacggggtgg?ggttggatgc?cctggtcgac?taccgctggg?aggtgtccct?cggcgaccag????1440

ccgctgaccg?ccgaggaact?ggagtcgctg?gccgcgctga?aatctccgtt?ggtccgcctg????1500

cgtgggcgct?gggtggagct?ggacccgaaa?cgtctcgccg?ccggcctgcg?gctgctccgt????1560

tccgccggcg?agctgaccgt?cggcgacctg?ctgcggctcg?gcctctccga?ccctgctacc????1620

gacgcgctgc?cggtgctcga?ggtggcggcc?gacggtgcgt?tgggtgactt?gctcgccgga????1680

gctgtggagc?ggcaactcac?cccggtggac?gcggttccgt?cgttccaggg?cgttctccgc????1740

ccctaccagc?ggcgagggct?ggcctggctg?tcctttctgc?agtccctcgg?cctcggcggg????1800

gtgctcgctg?acgacatggg?tctcggcaag?acggtacagc?tactcgcgtt?gctcgctggt????1860

gacccgccgg?gcgtcggtcc?gaccctgttg?gtctgtccga?tgtcactggt?cggtaactgg????1920

cagcgggagg?cggcgacctt?caccccgggc?gtacgggtcc?atgtgcatca?cggcgccgag????1980

cgggcccgcg?gggcggcgtt?caccgcggcg?gtggaggcag?cggacctggt?cctcaccacc????2040

tacacggtgg?ctgcccgcga?tgcgggggag?ctggccgggg?tcgactggca?tcgggtggtg????2100

gtggacgagg?cacaggccat?caagaacgcc?tcgacgcggc?aagccgaggc?ggtccgggcg????2160

ttgcccgccc?ggcatcggat?cgcggtcacc?ggcaccccgg?tggagaatcg?gctcgccgac????2220

ctctggtcga?tcatgcagtt?cgccaatccc?ggtctgctcg?gcccggccgc?cgagttcaag????2280

aagcggtacg?ccgaaccgat?cgagcgacac?ggcgacgcgg?aggcggccga?gcggctgcgc????2340

cggatcaccg?gcccgttcgt?gctgcgtcgc?ctcaagaccg?actcttcggt?tatctccgac????2400

ctgccagaga?agctggagat?ggaggtggtg?tgcaacctga?ccgcggaaca?ggctgccctc????2460

taccgtgcgg?tggtggacga?catgatggcc?cagatcgagt?ccagcgaggg?catcgagcga????2520

cgtgggctcg?tgctggccgc?catgacccgg?ctcaagcagg?tctgcaacca?cccggcgcac????2580

ctgctgcggg?acaactcggc?gctggtcggc?cgctccggca?agctggcccg?gctggaggag????2640

atcctcgacg?aggtgcttgt?cgcgggggag?aaggccctgc?tcttcaccca?gtacgccgag????2700

ttcggcggca?tgctgcgcgg?ccacctgtcg?gcccggttcg?gacaggagac?gctgttcctg????2760

cacggcggcg?tcggtaaggc?cgaccgggac?gcgatggtga?cgcggttcca?gtccccggac????2820

ggccccgcgc?tcttcgtact?ctcgctcaag?gccggtggta?ccggtctcac?cctgaccgcg????2880

gccaaccatg?tcgtgcacgt?tgaccgctgg?tggaatccgg?cggtggagga?ccaggccacg????2940

gaccgggcgt?tccgcatcgg?gcagcggcgg?cgcgttcagg?tccgcaagtt?tgtctgcgcc????3000

ggcacggtgg?aggagaaggt?cgccgcgctc?atcgccgaca?agcgtcggct?cgcctcgacg????3060

gtggtgggtg?ccggtgagca?gtgggttacc?gagctgtcca?cggcgcagct?gcgggagctg????3120

ttccagctgg?agtccggggc?ggtggccgaa?tga?????????????????????????????????3153

<210>78

<211>1050

<212>PRT

<213>Salinispora?tropica

<400>78

Val?Leu?Val?Val?His?Gly?Ser?Trp?Arg?Leu?Gly?Ile?Gly?Leu?Ala?Ile

1???????????????5???????????????????10??????????????????15

Trp?Ala?Glu?Asp?Ser?Ala?Ser?Pro?Pro?Arg?Ala?Pro?Arg?Arg?Ala?Gly

20??????????????????25??????????????????30

Arg?Ala?Pro?Arg?Glu?Arg?Pro?His?Pro?Phe?Ala?Ala?Gly?His?Pro?Val

35??????????????????40??????????????????45

Leu?Ala?Ala?Ala?Leu?Ala?Glu?Val?Ala?Glu?Pro?Thr?Glu?Pro?Gly?Thr

50??????????????????55??????????????????60

Ala?Leu?Leu?Thr?Leu?Pro?Thr?Arg?Ala?Gly?Ser?Pro?Leu?Asp?Ser?Pro

65??????????????????70??????????????????75??????????????????80

Glu?Leu?Val?Arg?Thr?Ala?Ser?Val?Glu?Pro?Leu?Arg?Gly?Pro?Val?Thr

85??????????????????90??????????????????95

Leu?Ala?Gly?Trp?Arg?Val?Pro?Ala?Leu?Val?Tyr?Ala?Pro?Asp?Ala?Ala

100?????????????????105?????????????????110

Leu?Ser?Leu?Leu?Ser?Gln?Ile?Thr?Ala?Ala?Gly?Ala?Leu?Pro?Asp?Ala

115?????????????????120?????????????????125

Val?Pro?Gly?Ala?Thr?Leu?Arg?His?Leu?Ala?Glu?Leu?Ala?Ala?Phe?Ala

130?????????????????135?????????????????140

Val?Asp?Leu?Ala?Ala?Arg?Gly?Arg?Val?Leu?Pro?Gly?Val?Arg?Pro?Pro

145?????????????????150?????????????????155?????????????????160

Lys?Glu?Arg?Ala?Ser?Ala?Ala?Trp?Ala?Val?Trp?Gln?Pro?Leu?Leu?Thr

165?????????????????170?????????????????175

Gly?Val?Asp?Ala?Gly?Trp?Ala?Arg?Ala?Leu?Ala?Leu?Ala?Leu?Pro?Pro

180?????????????????185?????????????????190

Ala?Val?Arg?Ala?Ala?Val?Glu?Ile?Asp?Pro?Ala?Pro?Leu?Ala?Val?Pro

195?????????????????200?????????????????205

Gly?Gly?Pro?Glu?Thr?Pro?Ala?Asn?Gly?Gly?Val?Pro?Pro?Gln?Ala?Arg

210?????????????????215?????????????????220

Thr?Arg?Arg?Pro?Thr?Ala?Ala?Ala?Gly?Glu?Pro?Gly?Glu?Leu?Val?Val

225?????????????????230?????????????????235?????????????????240

Glu?Ala?Leu?Asp?Ala?Leu?Thr?Asp?Ala?Ala?Val?Arg?Ala?Ala?Leu?Ala

245?????????????????250?????????????????255

Glu?Thr?Ser?Leu?Thr?Arg?Gly?Ala?Arg?Pro?Arg?Gly?Ala?Val?Ala?Ala

260?????????????????265?????????????????270

Trp?Leu?Ala?Ala?Leu?Thr?Gly?Pro?Arg?Arg?Asp?Phe?Thr?Ala?Asp?Ser

275?????????????????280?????????????????285

Ala?Glu?Leu?Asp?Thr?Leu?Arg?Gly?Glu?Leu?Asp?Ala?Trp?Gln?Arg?Asp

290?????????????????295?????????????????300

Ala?Val?Gly?Gly?Ser?Val?Arg?Ala?Ser?Phe?Arg?Leu?Val?Glu?Pro?Pro

305?????????????????310?????????????????315?????????????????320

Thr?Asp?Gly?Leu?Phe?Glu?Ala?Ala?Ala?Gly?Gly?Leu?Ala?Ala?Ala?Glu

325?????????????????330?????????????????335

Gly?Ser?Trp?Arg?Val?Glu?Phe?Gly?Leu?Gln?Pro?Ala?Asp?Gln?Pro?Gly

340?????????????????345?????????????????350

Leu?His?Val?Asp?Ala?Val?Arg?Ile?Trp?His?Glu?Ser?Ala?Ala?Leu?Pro

355?????????????????360?????????????????365

Gly?Pro?Ala?Ala?Pro?Gln?Glu?Ala?Leu?Leu?Thr?Glu?Leu?Gly?Arg?Ala

370?????????????????375?????????????????380

Ser?Arg?Leu?Trp?Pro?Glu?Leu?Asn?Ser?Ala?Leu?Arg?Thr?Ala?Thr?Pro

385?????????????????390?????????????????395?????????????????400

Glu?Ala?Leu?Glu?Leu?Asp?Ala?Ala?Gly?Ala?His?Arg?Phe?Leu?Arg?Asp

405?????????????????410?????????????????415

Gly?Ala?Pro?Val?Leu?His?Ala?Ala?Gly?Phe?Ala?Val?Leu?Leu?Pro?Ser

420?????????????????425?????????????????430

Trp?Trp?Gln?Arg?Pro?Ser?Ser?Arg?Leu?Gly?Ala?Arg?Leu?Gln?Ala?Gln

435?????????????????440?????????????????445

Ser?Arg?Thr?Ala?Pro?Gly?Thr?Val?Ala?Gly?Ala?Gly?Asp?Gly?Val?Gly

450?????????????????455?????????????????460

Leu?Asp?Ala?Leu?Val?Asp?Tyr?Arg?Trp?Glu?Val?Ser?Leu?Gly?Asp?Gln

465?????????????????470?????????????????475?????????????????480

Pro?Leu?Thr?Ala?Glu?Glu?Leu?Glu?Ser?Leu?Ala?Ala?Leu?Lys?Ser?Pro

485?????????????????490?????????????????495

Leu?Val?Arg?Leu?Arg?Gly?Arg?Trp?Val?Glu?Leu?Asp?Pro?Lys?Arg?Leu

500?????????????????505?????????????????510

Ala?Ala?Gly?Leu?Arg?Leu?Leu?Arg?Ser?Ala?Gly?Glu?Leu?Thr?Val?Gly

515?????????????????520?????????????????525

Asp?Leu?Leu?Arg?Leu?Gly?Leu?Ser?Asp?Pro?Ala?Thr?Asp?Ala?Leu?Pro

530?????????????????535?????????????????540

Val?Leu?Glu?Val?Ala?Ala?Asp?Gly?Ala?Leu?Gly?Asp?Leu?Leu?Ala?Gly

545?????????????????550?????????????????555?????????????????560

Ala?Val?Glu?Arg?Gln?Leu?Thr?Pro?Val?Asp?Ala?Val?Pro?Ser?Phe?Gln

565?????????????????570?????????????????575

Gly?Val?Leu?Arg?Pro?Tyr?Gln?Arg?Arg?Gly?Leu?Ala?Trp?Leu?Ser?Phe

580?????????????????585?????????????????590

Leu?Gln?Ser?Leu?Gly?Leu?Gly?Gly?Val?Leu?Ala?Asp?Asp?Met?Gly?Leu

595?????????????????600?????????????????605

Gly?Lys?Thr?Val?Gln?Leu?Leu?Ala?Leu?Leu?Ala?Gly?Asp?Pro?Pro?Gly

610?????????????????615?????????????????620

Val?Gly?Pro?Thr?Leu?Leu?Val?Cys?Pro?Met?Ser?Leu?Val?Gly?Asn?Trp

625?????????????????630?????????????????635?????????????????640

Gln?Arg?Glu?Ala?Ala?Thr?Phe?Thr?Pro?Gly?Val?Arg?Val?His?Val?His

645?????????????????650?????????????????655

His?Gly?Ala?Glu?Arg?Ala?Arg?Gly?Ala?Ala?Phe?Thr?Ala?Ala?Val?Glu

660?????????????????665?????????????????670

Ala?Ala?Asp?Leu?Val?Leu?Thr?Thr?Tyr?Thr?Val?Ala?Ala?Arg?Asp?Ala

675?????????????????680?????????????????685

Gly?Glu?Leu?Ala?Gly?Val?Asp?Trp?His?Arg?Val?Val?Val?Asp?Glu?Ala

690?????????????????695?????????????????700

Gln?Ala?Ile?Lys?Asn?Ala?Ser?Thr?Arg?Gln?Ala?Glu?Ala?Val?Arg?Ala

705?????????????????710?????????????????715?????????????????720

Leu?Pro?Ala?Arg?His?Arg?Ile?Ala?Val?Thr?Gly?Thr?Pro?Val?Glu?Asn

725?????????????????730?????????????????735

Arg?Leu?Ala?Asp?Leu?Trp?Ser?Ile?Met?Gln?Phe?Ala?Asn?Pro?Gly?Leu

740?????????????????745?????????????????750

Leu?Gly?Pro?Ala?Ala?Glu?Phe?Lys?Lys?Arg?Tyr?Ala?Glu?Pro?Ile?Glu

755?????????????????760?????????????????765

Arg?His?Gly?Asp?Ala?Glu?Ala?Ala?Glu?Arg?Leu?Arg?Arg?Ile?Thr?Gly

770?????????????????775?????????????????780

Pro?Phe?Val?Leu?Arg?Arg?Leu?Lys?Thr?Asp?Ser?Ser?Val?Ile?Ser?Asp

785?????????????????790?????????????????795?????????????????800

Leu?Pro?Glu?Lys?Leu?Glu?Met?Glu?Val?Val?Cys?Asn?Leu?Thr?Ala?Glu

805?????????????????810?????????????????815

Gln?Ala?Ala?Leu?Tyr?Arg?Ala?Val?Val?Asp?Asp?Met?Met?Ala?Gln?Ile

820?????????????????825?????????????????830

Glu?Ser?Ser?Glu?Gly?Ile?Glu?Arg?Arg?Gly?Leu?Val?Leu?Ala?Ala?Met

835?????????????????840?????????????????845

Thr?Arg?Leu?Lys?Gln?Val?Cys?Asn?His?Pro?Ala?His?Leu?Leu?Arg?Asp

850?????????????????855?????????????????860

Asn?Ser?Ala?Leu?Val?Gly?Arg?Ser?Gly?Lys?Leu?Ala?Arg?Leu?Glu?Glu

865?????????????????870?????????????????875?????????????????880

Ile?Leu?Asp?Glu?Val?Leu?Val?Ala?Gly?Glu?Lys?Ala?Leu?Leu?Phe?Thr

885?????????????????890?????????????????895

Gln?Tyr?Ala?Glu?Phe?Gly?Gly?Met?Leu?Arg?Gly?His?Leu?Ser?Ala?Arg

900?????????????????905?????????????????910

Phe?Gly?Gln?Glu?Thr?Leu?Phe?Leu?His?Gly?Gly?Val?Gly?Lys?Ala?Asp

915?????????????????920?????????????????925

Arg?Asp?Ala?Met?Val?Thr?Arg?Phe?Gln?Ser?Pro?Asp?Gly?Pro?Ala?Leu

930?????????????????935?????????????????940

Phe?Val?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Thr?Gly?Leu?Thr?Leu?Thr?Ala

945?????????????????950?????????????????955?????????????????960

Ala?Asn?His?Val?Val?His?Val?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu

965?????????????????970?????????????????975

Asp?Gln?Ala?Thr?Asp?Arg?Ala?Phe?Arg?Ile?Gly?Gln?Arg?Arg?Arg?Val

980?????????????????985?????????????????990

Gln?Val?Arg?Lys?Phe?Val?Cys?Ala??Gly?Thr?Val?Glu?Glu??Lys?Val?Ala

995?????????????????1000?????????????????1005

Ala?Leu??Ile?Ala?Asp?Lys?Arg??Arg?Leu?Ala?Ser?Thr??Val?Val?Gly

1010?????????????????1015?????????????????1020

Ala?Gly??Glu?Gln?Trp?Val?Thr??Glu?Leu?Ser?Thr?Ala??Gln?Leu?Arg

1025?????????????????1030?????????????????1035

Glu?Leu??Phe?Gln?Leu?Glu?Ser??Gly?Ala?Val?Ala?Glu

1040?????????????????1045?????????????????1050

<210>79

<211>2970

<212>DNA

<213>Symbiobacterium?thermophilum

<400>79

atgatcacgg?ttcacggcag?tttcgtcccc?tccggcgcgt?ccggcttctt?cttcctgtgg?????60

ggcctggacg?gcgtggccgc?ccgggatgcc?gctcctcccg?gccggcgccg?ccgcggggtt????120

ccgcgccacc?catgcgcaac?cgagccggaa?gcgctctacc?ccgccctgag?aggattgccc????180

tacctgaaca?ccctgtccct?ggtccagtgg?cagcccggac?cggacggcgt?cagcccggcc????240

cgggtcccgg?ggatcgccct?gtccgtgccc?aacgccgtgc?agtggctgtt?ggatctgccc????300

gaccacttcc?gcggcacgcc?cctccggccg?gggcacagcc?tgcagctctg?gtgcgtcgca????360

tccaagctgc?ttctggagtt?cctggggcgg?ggcctgatgc?tgccggtgct?gcaggccgag????420

gccggggtgc?tgagcgcggg?ctgggcgctc?cacctgaccg?acgccgacga?cgtccgccgc????480

ctgacccggc?tggccgctgg?attgccggag?gcctgccgcg?cccttgtgcc?ccccgaccga????540

acccccaaca?cctaccccct?gccggtcgcc?gacggcctgg?tccaccagtt?catgcgtacg????600

gcggccgccg?gcgtgatccg?gctcctcctg?gaggaagagc?ccctgcccga?ggcccagtcg????660

ctacaggata?ccgccctgcg?ccactggctg?gcggcgctga?ccggggcgga?ggcccgggac????720

ctgccgccgg?gcctgcccgg?cgcgcaggag?ctgtacgccg?ccctggaccg?ctggagcgcc????780

cccgccaccg?gcgtgctgag?ccacgccagt?ctgcggacgg?gggtccgcct?ccacctgccc????840

ggccccgaga?ccgacggcga?gtgggagctg?gagctcacgc?tccatgcgcc?ggacgagggt????900

gcgctgcccg?tcaccgccga?tgcggtctgg?gccagcctgg?gcgccgaggt?ggagatcggc????960

gggcagcggt?accagggcgc?cgagcagcgg?ctgctggccg?acctgccggc?catggcccgc???1020

ctcttcccgc?cactggcgcc?gctgctccgg?gaccccgcgc?ccagccgcat?gcgcattccg???1080

gcggacgacg?tgctggccct?gatccaggaa?ggggccatgc?tgctccagca?ggccggccac???1140

cccgtgctgc?tgccggccgc?ccttgcgaag?cccgccgccc?tccgggtcgg?aatgcgcctc???1200

agccccgccg?ggggcagccc?ctccatgttc?gggctgcacc?agatcgtgaa?cgtgcgctgg???1260

gacgtggccc?tgggcggcac?cccgctcacg?ctggacgagc?tgcgccacct?ggcgcggcag???1320

aagcggcccc?tggtacagat?gcagggccgg?tgggtgcggg?tggacgaacg?caccctggct????1380

gcggtcctcc?gccggatcga?gcagcacggc?gggcagatgg?agctgggcac?ggcgctgcgc????1440

ctggcacccg?aggcggacga?ggccaccgcg?accggctgga?tcgccgagct?gctggagcgg????1500

ctgcaggagc?cagcccggat?ggagccggtg?ccgacccccg?ggggcttcgc?cggcaccctg????1560

cggccgtacc?agcagcgggg?cctcgcctgg?ctggcgttcc?tgcgccgctg?gggcctgggc????1620

gcgtgcctcg?ccgacgacat?ggggctgggc?aagaccgtgc?agctcatcgc?ccttctcctg????1680

cacgagcggg?aggccgggtg?ggccgcgggc?ccgaccctgc?tggtctgccc?cgtctcggtc????1740

ctgggcaact?ggtgccggga?gctggcccgc?ttcgccccgg?gcctgcgggt?cctggtgcac????1800

catggccccg?ggaggctggg?cgagccggac?ttcgcccggc?aggccggggc?ccacgacgtg????1860

gtgctgacca?cgtactccct?gctggcccgg?gatgccgcgc?tgctgggcca?ggtgacctgg????1920

aacgggatcg?tcgccgacga?ggcgcagaac?ctgaaaaacc?ccgacacaca?gcacgcccgg????1980

gcgctgcgaa?gcctttccgg?cggctaccgc?atcgccctca?ccggtacgcc?cgtcgaaaac????2040

cacctgggcg?acctgtggtc?gctcttccag?ttcctcaacc?cggggctgct?gggcagccgc????2100

gaggagttcg?agcggcgcta?cgccgtgccg?atccagcggt?accaggacga?ggaggctgcg????2160

gcccggctcc?gccggcaggt?gggtcccttc?atcctgcgcc?ggcagaagaa?cgaccccgcc????2220

atcgcgccgg?acctgcccga?caagctggag?aacaccgagc?tggtgaccct?ctcggtggaa????2280

caggcggcgc?tgtacgaggc?catcgtgcag?gagacgctgg?agcgggccgc?gcaggccgac????2340

ggcatccagc?ggcaggcggc?ggtcctggca?ggcctcacgc?ggctgaagca?ggtgtgcaac????2400

catcccgcag?ccgccaccgg?cgacggcccc?ctggtggggc?ggagcggcaa?gatcgaccgg????2460

ctggtgcaac?tgctgcagga?ggtgctggcg?gcgggcgagc?aggccctgct?cttcacccag????2520

ttcgcccgct?tcggcgggcg?gctgcaggcc?tacctggcgg?agacgctggg?ctgcgaggtg????2580

ctcttcctgc?acggcggcac?gccccagccc?gagcgggacc?ggctcgtcgc?ccggttccag????2640

gccggcgagg?cgcccctctt?catcctctcg?ctgaaagccg?gcggccttgg?cctcaacctc????2700

accgccgcga?cccacgtctt?tcacgtggac?cggtggtgga?atccggcggt?ggaggatcag????2760

gccacagacc?gggcctaccg?catcggccag?acgcgcaggg?tgctggtgca?ccggctgatc????2820

accgccggca?cgctggagga?gcgcatcgac?cggctgctgg?ccgagaagcg?tgccctggcg????2880

ggccaggtga?tcatcagcgg?cgagtcgtgg?ctcggccagc?tctccaccga?ggagctgcgg????2940

gccctgatcg?ccctggaccg?ggaggtgtag?????????????????????????????????????2970

<210>80

<211>989

<212>PRT

<213>Symbiobacterium?thermophilum

<400>80

Met?Ile?Thr?Val?His?Gly?Ser?Phe?Val?Pro?Ser?Gly?Ala?Ser?Gly?Phe

1???????????????5???????????????????10??????????????????15

Phe?Phe?Leu?Trp?Gly?Leu?Asp?Gly?Val?Ala?Ala?Arg?Asp?Ala?Ala?Pro

20??????????????????25??????????????????30

Pro?Gly?Arg?Arg?Arg?Arg?Gly?Val?Pro?Arg?His?Pro?Cys?Ala?Thr?Glu

35??????????????????40??????????????????45

Pro?Glu?Ala?Leu?Tyr?Pro?Ala?Leu?Arg?Gly?Leu?Pro?Tyr?Leu?Asn?Thr

50??????????????????55??????????????????60

Leu?Ser?Leu?Val?Gln?Trp?Gln?Pro?Gly?Pro?Asp?Gly?Val?Ser?Pro?Ala

65??????????????????70??????????????????75??????????????????80

Arg?Val?Pro?Gly?Ile?Ala?Leu?Ser?Val?Pro?Asn?Ala?Val?Gln?Trp?Leu

85??????????????????90??????????????????95

Leu?Asp?Leu?Pro?Asp?His?Phe?Arg?Gly?Thr?Pro?Leu?Arg?Pro?Gly?His

100?????????????????105?????????????????110

Ser?Leu?Gln?Leu?Trp?Cys?Val?Ala?Ser?Lys?Leu?Leu?Leu?Glu?Phe?Leu

115?????????????????120?????????????????125

Gly?Arg?Gly?Leu?Met?Leu?Pro?Val?Leu?Gln?Ala?Glu?Ala?Gly?Val?Leu

130?????????????????135?????????????????140

Ser?Ala?Gly?Trp?Ala?Leu?His?Leu?Thr?Asp?Ala?Asp?Asp?Val?Arg?Arg

145?????????????????150?????????????????155?????????????????160

Leu?Thr?Arg?Leu?Ala?Ala?Gly?Leu?Pro?Glu?Ala?Cys?Arg?Ala?Leu?Val

165?????????????????170?????????????????175

Pro?Pro?Asp?Arg?Thr?Pro?Asn?Thr?Tyr?Pro?Leu?Pro?Val?Ala?Asp?Gly

180?????????????????185?????????????????190

Leu?Val?His?Gln?Phe?Met?Arg?Thr?Ala?Ala?Ala?Gly?Val?Ile?Arg?Leu

195?????????????????200?????????????????205

Leu?Leu?Glu?Glu?Glu?Pro?Leu?Pro?Glu?Ala?Gln?Ser?Leu?Gln?Asp?Thr

210?????????????????215?????????????????220

Ala?Leu?Arg?His?Trp?Leu?Ala?Ala?Leu?Thr?Gly?Ala?Glu?Ala?Arg?Asp

225?????????????????230?????????????????235?????????????????240

Leu?Pro?Pro?Gly?Leu?Pro?Gly?Ala?Gln?Glu?Leu?Tyr?Ala?Ala?Leu?Asp

245?????????????????250?????????????????255

Arg?Trp?Ser?Ala?Pro?Ala?Thr?Gly?Val?Leu?Ser?His?Ala?Ser?Leu?Arg

260?????????????????265?????????????????270

Thr?Gly?Val?Arg?Leu?His?Leu?Pro?Gly?Pro?Glu?Thr?Asp?Gly?Glu?Trp

275?????????????????280?????????????????285

Glu?Leu?Glu?Leu?Thr?Leu?His?Ala?Pro?Asp?Glu?Gly?Ala?Leu?Pro?Val

290?????????????????295?????????????????300

Thr?Ala?Asp?Ala?Val?Trp?Ala?Ser?Leu?Gly?Ala?Glu?Val?Glu?Ile?Gly

305?????????????????310?????????????????315?????????????????320

Gly?Gln?Arg?Tyr?Gln?Gly?Ala?Glu?Gln?Arg?Leu?Leu?Ala?Asp?Leu?Pro

325?????????????????330?????????????????335

Ala?Met?Ala?Arg?Leu?Phe?Pro?Pro?Leu?Ala?Pro?Leu?Leu?Arg?Asp?Pro

340?????????????????345?????????????????350

Ala?Pro?Ser?Arg?Met?Arg?Ile?Pro?Ala?Asp?Asp?Val?Leu?Ala?Leu?Ile

355?????????????????360?????????????????365

Gln?Glu?Gly?Ala?Met?Leu?Leu?Gln?Gln?Ala?Gly?His?Pro?Val?Leu?Leu

370?????????????????375?????????????????380

Pro?Ala?Ala?Leu?Ala?Lys?Pro?Ala?Ala?Leu?Arg?Val?Gly?Met?Arg?Leu

385?????????????????390?????????????????395?????????????????400

Ser?Pro?Ala?Gly?Gly?Ser?Pro?Ser?Met?Phe?Gly?Leu?His?Gln?Ile?Val

405?????????????????410?????????????????415

Asn?Val?Arg?Trp?Asp?Val?Ala?Leu?Gly?Gly?Thr?Pro?Leu?Thr?Leu?Asp

420?????????????????425?????????????????430

Glu?Leu?Arg?His?Leu?Ala?Arg?Gln?Lys?Arg?Pro?Leu?Val?Gln?Met?Gln

435?????????????????440?????????????????445

Gly?Arg?Trp?Val?Arg?Val?Asp?Glu?Arg?Thr?Leu?Ala?Ala?Val?Leu?Arg

450?????????????????455?????????????????460

Arg?Ile?Glu?Gln?His?Gly?Gly?Gln?Met?Glu?Leu?Gly?Thr?Ala?Leu?Arg

465?????????????????470?????????????????475?????????????????480

Leu?Ala?Pro?Glu?Ala?Asp?Glu?Ala?Thr?Ala?Thr?Gly?Trp?Ile?Ala?Glu

485?????????????????490?????????????????495

Leu?Leu?Glu?Arg?Leu?Gln?Glu?Pro?Ala?Arg?Met?Glu?Pro?Val?Pro?Thr

500?????????????????505?????????????????510

Pro?Gly?Gly?Phe?Ala?Gly?Thr?Leu?Arg?Pro?Tyr?Gln?Gln?Arg?Gly?Leu

515?????????????????520?????????????????525

Ala?Trp?Leu?Ala?Phe?Leu?Arg?Arg?Trp?Gly?Leu?Gly?Ala?Cys?Leu?Ala

530?????????????????535?????????????????540

Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Val?Gln?Leu?Ile?Ala?Leu?Leu?Leu

545?????????????????550?????????????????555?????????????????560

His?Glu?Arg?Glu?Ala?Gly?Trp?Ala?Ala?Gly?Pro?Thr?Leu?Leu?Val?Cys

565?????????????????570?????????????????575

Pro?Val?Ser?Val?Leu?Gly?Asn?Trp?Cys?Arg?Glu?Leu?Ala?Arg?Phe?Ala

580?????????????????585?????????????????590

Pro?Gly?Leu?Arg?Val?Leu?Val?His?His?Gly?Pro?Gly?Arg?Leu?Gly?Glu

595?????????????????600?????????????????605

Pro?Asp?Phe?Ala?Arg?Gln?Ala?Gly?Ala?His?Asp?Val?Val?Leu?Thr?Thr

610?????????????????615?????????????????620

Tyr?Ser?Leu?Leu?Ala?Arg?Asp?Ala?Ala?Leu?Leu?Gly?Gln?Val?Thr?Trp

625?????????????????630?????????????????635?????????????????640

Asn?Gly?Ile?Val?Ala?Asp?Glu?Ala?Gln?Asn?Leu?Lys?Asn?Pro?Asp?Thr

645?????????????????650?????????????????655

Gln?His?Ala?Arg?Ala?Leu?Arg?Ser?Leu?Ser?Gly?Gly?Tyr?Arg?Ile?Ala

660?????????????????665?????????????????670

Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?His?Leu?Gly?Asp?Leu?Trp?Ser?Leu

675?????????????????680?????????????????685

Phe?Gln?Phe?Leu?Asn?Pro?Gly?Leu?Leu?Gly?Ser?Arg?Glu?Glu?Phe?Glu

690?????????????????695?????????????????700

Arg?Arg?Tyr?Ala?Val?Pro?Ile?Gln?Arg?Tyr?Gln?Asp?Glu?Glu?Ala?Ala

705?????????????????710?????????????????715?????????????????720

Ala?Arg?Leu?Arg?Arg?Gln?Val?Gly?Pro?Phe?Ile?Leu?Arg?Arg?Gln?Lys

725?????????????????730?????????????????735

Asn?Asp?Pro?Ala?Ile?Ala?Pro?Asp?Leu?Pro?Asp?Lys?Leu?Glu?Asn?Thr

740?????????????????745?????????????????750

Glu?Leu?Val?Thr?Leu?Ser?Val?Glu?Gln?Ala?Ala?Leu?Tyr?Glu?Ala?Ile

755?????????????????760?????????????????765

Val?Gln?Glu?Thr?Leu?Glu?Arg?Ala?Ala?Gln?Ala?Asp?Gly?Ile?Gln?Arg

770?????????????????775?????????????????780

Gln?Ala?Ala?Val?Leu?Ala?Gly?Leu?Thr?Arg?Leu?Lys?Gln?Val?Cys?Asn

785?????????????????790?????????????????795?????????????????800

His?Pro?Ala?Ala?Ala?Thr?Gly?Asp?Gly?Pro?Leu?Val?Gly?Arg?Ser?Gly

805?????????????????810?????????????????815

Lys?Ile?Asp?Arg?Leu?Val?Gln?Leu?Leu?Gln?Glu?Val?Leu?Ala?Ala?Gly

820?????????????????825?????????????????830

Glu?Gln?Ala?Leu?Leu?Phe?Thr?Gln?Phe?Ala?Arg?Phe?Gly?Gly?Arg?Leu

835?????????????????840?????????????????845

Gln?Ala?Tyr?Leu?Ala?Glu?Thr?Leu?Gly?Cys?Glu?Val?Leu?Phe?Leu?His

850?????????????????855?????????????????860

Gly?Gly?Thr?Pro?Gln?Pro?Glu?Arg?Asp?Arg?Leu?Val?Ala?Arg?Phe?Gln

865?????????????????870?????????????????875?????????????????880

Ala?Gly?Glu?Ala?Pro?Leu?Phe?Ile?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Leu

885?????????????????890?????????????????895

Gly?Leu?Asn?Leu?Thr?Ala?Ala?Thr?His?Val?Phe?His?Val?Asp?Arg?Trp

900?????????????????905?????????????????910

Trp?Asn?Pro?Ala?Val?Glu?Asp?Gln?Ala?Thr?Asp?Arg?Ala?Tyr?Arg?Ile

915?????????????????920?????????????????925

Gly?Gln?Thr?Arg?Arg?Val?Leu?Val?His?Arg?Leu?Ile?Thr?Ala?Gly?Thr

930?????????????????935?????????????????940

Leu?Glu?Glu?Arg?Ile?Asp?Arg?Leu?Leu?Ala?Glu?Lys?Arg?Ala?Leu?Ala

945?????????????????950?????????????????955?????????????????960

Gly?Gln?Val?Ile?Ile?Ser?Gly?Glu?Ser?Trp?Leu?Gly?Gln?Leu?Ser?Thr

965?????????????????970?????????????????975

Glu?Glu?Leu?Arg?Ala?Leu?Ile?Ala?Leu?Asp?Arg?Glu?Val

980?????????????????985

<210>81

<211>3114

<212>DNA

＜213〉synechococcus

<400>81

atgagcctgc?tgcacgccac?ctggctgtcg?gccgacaccg?ccgccgtgcc?cgccctggga?????60

ggcggctacc?ggccgggctt?gctgctctgg?gccgacacct?ggcgggtggc?ggaaccccag????120

acaccggcca?gcgaggcgcc?ccagcacccc?ctcagcctcg?accaggacga?cctcggcgcc????180

tggcttgagg?aggccgacct?ctggacggag?gatttccgcc?cggccggagc?caccctctgc????240

ctgcccagcc?gccgccaggg?ggccaggggg?aaaaagaaaa?gcgacaccag?cagctggagc????300

ggcctgcccc?tgcaggcggg?cgagccgatc?ccgaaatccg?tggagtggtg?gccctggcgg????360

gtggagggct?ggtggctgga?gcccggcgcc?gccaccctct?ggcttgggcg?cctgcccctc????420

tcaggcgacc?atcccgacct?ggccgatgac?ctgcgctggt?ggagccatct?gcagcgctgg????480

tcgctgagcc?tgctggcccg?gggccggctg?ctgccccagg?tggagggggg?ccgcgcccgc????540

tggctgccgt?tgatcaaccg?cgaagacgac?cggcgccgcc?tggaggatct?ggcctcgcgt????600

ctgccccagg?tggcggtggc?ggccctggag?cccggccagg?gggaggccgg?cgtcgcgatg????660

gcgtgctggc?ggccgggatc?cgggcgtcgg?cggctggcct?cgatcctcac?gcacctggtg????720

gatgcacgca?tgcgtgcggg?cttcaccccc?agcgaagagg?ggctggatcc?gctgctggcg????780

gcctggcagc?gggccctcgg?ccccggtgac?ggccgcctcg?atctcgggga?cgacgactgc????840

gaacgcctgc?aggtggccac?tcaccactgg?cgcgaagcgg?tggctggccg?ggtcgagccg????900

gcccgggcct?gtcttgagct?cgacacaccc?gatgaggggg?aagatctctg?gcccctgcgc????960

ttcagcctcc?aggccgaggc?cgatcccagt?ctgctgctgc?ccgcagccgg?ggtctgggcc???1020

gccggggccg?gctgcctgca?gctgggtgaa?accgaactcc?agcaacccgg?tgaactgctg????1080

ctggaaggcc?tcgggagagc?cctgcaggtg?ttcgagccga?tcgagagggg?tctcgacacc????1140

gccacaccgg?agcggatggc?tctcaccccg?gccgaagcct?tcgtgctggt?gcgcaccgcc????1200

gcgctgaagc?tgcgtgatgt?gggcgtcggc?gtggtcctgc?cccccagcct?cagcggtggc????1260

ctggccagcc?ggctcggcct?ctcgatcgag?gccgatctgc?ccgagcgctc?ccgcggcttc????1320

agcctcggtg?aaagcctgca?gtggagctgg?gagctgatga?tcggcggcgt?cacgctcacc????1380

ctgcgggacc?tggagcggct?ggcgggcaag?cgcagcccgc?tggtgcagca?caagggggcc????1440

tggatcgagc?tgcgtccggg?tgatctgcgc?aatgccgaga?agttctgcgc?cctcgatccg????1500

gtcctcagcc?tcgatgacgc?cctgcgcctg?accggcaacg?agggggagac?cctgcagcgg????1560

ctgccggtgc?accgcttcac?agccggcccg?aggctgaagg?cggtgctgga?gcagtaccac????1620

cagcagaagg?cccccgatcc?cctgccggcc?cccgagggct?tcgccggcca?gctgcggccc????1680

taccaggagc?gcggcctggg?ctggctggcc?ttcctgcacc?gcttcgatca?gggggcctgc????1740

ctggccgacg?acatgggcct?gggcaagaca?atccagctgc?tggccttcct?gcagcacctc????1800

aaggcggagc?aggaactgaa?gcgtcccgta?ctgctggtgg?cccccacctc?ggtgctcacc????1860

aactggctgc?gggaagcgaa?ggccttcacg?ccggaactga?acgtggtgga?gcactacggc????1920

ccccggcggc?cctccacccc?cgccgccctg?aagaagaagc?tggaggggat?ggatctggtg????1980

ctcaccagct?acggcctgct?gcagcgcgac?agcgagttac?tgagcagcct?cgactggcag????2040

ggggtggtga?ttgatgaggc?ccaggcgatc?aagaattcct?cagcgcgcca?gtcgcaggca????2100

gcccgcgatc?tggcacgccc?gctcaagcag?agccgcttcc?gtatcgcact?caccggcacc????2160

ccggtggaga?accgggtcag?tgagctctgg?gccctgatgg?acttcctcaa?tccgaaggtg????2220

cttggggagg?aggagttctt?ccgccagcgc?taccgcctgc?cgatcgagcg?ctatggcgac????2280

atggcctcgg?tgcgcgacct?caaggcccgc?gtcggcccgt?tcatcctgcg?gcgcctcaag????2340

actgaccgct?cgatcatctc?cgacctgccc?gagaaggtgg?aactgaagga?gtgggttgga??2400

ctctcacccg?agcaggtcaa?gctctaccgc?cgcaccgtgg?aggacaccct?cgatgcgatc??2460

gcgcgggcac?ccgtgggcca?gaagcacggc?caggtgctgg?ggctgctcac?caagctcaag??2520

caggtctgca?accacccggc?cctgatgctc?aaggaagggg?aggtgggggc?cggcttcagc??2580

gcccgctcgg?ccaagttgca?gcggctcgag?gaaatcgtcg?aggaggtgat?cgcggccggc??2640

gatcgggccc?tcctgtttac?ccagttcgcc?gaatggggcc?acctgctcca?gacccacctg??2700

cagcagcgct?tccaccagga?ggtgcccttt?ctctatggca?gtaccagcaa?gggggagcgt??2760

caggcgatgg?tggatcgctt?ccaggacgac?ccccggggac?cacagctgtt?cctgctctcg??2820

ctcaaggcag?gcggcgtggg?gctcaacctc?acccgggcca?gtcatgtgtt?ccacatcgac??2880

cgctggtgga?atccggcggt?ggagaaccag?gccaccgacc?gggcctaccg?catcggccag??2940

accaaccggg?tgatggtgca?caagttcatc?accagcggct?cggtggagga?gaagatcgac??3000

cgcatgatcc?gcgaaaaggc?ccgcctggcc?gaagacatcg?tcggcagcgg?tgaggagtgg??3060

ctcggaggcc?tcgatcccgg?ccagctgcgc?gacctggtgg?ccctggagga?gtga????????3114

<210>82

<211>1037

<212>PRT

＜213〉synechococcus

<400>82

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Ser?Ala?Asp?Thr?Ala?Ala?Val

1???????????????5???????????????????10??????????????????15

Pro?Ala?Leu?Gly?Gly?Gly?Tyr?Arg?Pro?Gly?Leu?Leu?Leu?Trp?Ala?Asp

20??????????????????25??????????????????30

Thr?Trp?Arg?Val?Ala?Glu?Pro?Gln?Thr?Pro?Ala?Ser?Glu?Ala?Pro?Gln

35??????????????????40??????????????????45

His?Pro?Leu?Ser?Leu?Asp?Gln?Asp?Asp?Leu?Gly?Ala?Trp?Leu?Glu?Glu

50??????????????????55??????????????????60

Ala?Asp?Leu?Trp?Thr?Glu?Asp?Phe?Arg?Pro?Ala?Gly?Ala?Thr?Leu?Cys

65??????????????????70?????????????????75?????????????????80

Leu?Pro?Ser?Arg?Arg?Gln?Gly?Ala?Arg?Gly?Lys?Lys?Lys?Ser?Asp?Thr

85?????????????????90?????????????????95

Ser?Ser?Trp?Ser?Gly?Leu?Pro?Leu?Gln?Ala?Gly?Glu?Pro?Ile?Pro?Lys

100?????????????????105?????????????????110

Ser?Val?Glu?Trp?Trp?Pro?Trp?Arg?Val?Glu?Gly?Trp?Trp?Leu?Glu?Pro

115?????????????????120?????????????????125

Gly?Ala?Ala?Thr?Leu?Trp?Leu?Gly?Arg?Leu?Pro?Leu?Ser?Gly?Asp?His

130?????????????????135?????????????????140

Pro?Asp?Leu?Ala?Asp?Asp?Leu?Arg?Trp?Trp?Ser?His?Leu?Gln?Arg?Trp

145?????????????????150?????????????????155?????????????????160

Ser?Leu?Ser?Leu?Leu?Ala?Arg?Gly?Arg?Leu?Leu?Pro?Gln?Val?Glu?Gly

165?????????????????170?????????????????175

Gly?Arg?Ala?Arg?Trp?Leu?Pro?Leu?Ile?Asn?Arg?Glu?Asp?Asp?Arg?Arg

180?????????????????185?????????????????190

Arg?Leu?Glu?Asp?Leu?Ala?Ser?Arg?Leu?Pro?Gln?Val?Ala?Val?Ala?Ala

195?????????????????200?????????????????205

Leu?Glu?Pro?Gly?Gln?Gly?Glu?Ala?Gly?Val?Ala?Met?Ala?Cys?Trp?Arg

210?????????????????215?????????????????220

Pro?Gly?Ser?Gly?Arg?Arg?Arg?Leu?Ala?Ser?Ile?Leu?Thr?His?Leu?Val

225?????????????????230?????????????????235?????????????????240

Asp?Ala?Arg?Met?Arg?Ala?Gly?Phe?Thr?Pro?Ser?Glu?Glu?Gly?Leu?Asp

245?????????????????250?????????????????255

Pro?Leu?Leu?Ala?Ala?Trp?Gln?Arg?Ala?Leu?Gly?Pro?Gly?Asp?Gly?Arg

260?????????????????265?????????????????270

Leu?Asp?Leu?Gly?Asp?Asp?Asp?Cys?Glu?Arg?Leu?Gln?Val?Ala?Thr?His

275?????????????????280?????????????????285

His?Trp?Arg?Glu?Ala?Val?Ala?Gly?Arg?Val?Glu?Pro?Ala?Arg?Ala?Cys

290?????????????????295?????????????????300

Leu?Glu?Leu?Asp?Thr?Pro?Asp?Glu?Gly?Glu?Asp?Leu?Trp?Pro?Leu?Arg

305?????????????????310?????????????????315?????????????????320

Phe?Ser?Leu?Gln?Ala?Glu?Ala?Asp?Pro?Ser?Leu?Leu?Leu?Pro?Ala?Ala

325?????????????????330?????????????????335

Gly?Val?Trp?Ala?Ala?Gly?Ala?Gly?Cys?Leu?Gln?Leu?Gly?Glu?Thr?Glu

340?????????????????345?????????????????350

Leu?Gln?Gln?Pro?Gly?Glu?Leu?Leu?Leu?Glu?Gly?Leu?Gly?Arg?Ala?Leu

355?????????????????360?????????????????365

Gln?Val?Phe?Glu?Pro?Ile?Glu?Arg?Gly?Leu?Asp?Thr?Ala?Thr?Pro?Glu

370?????????????????375?????????????????380

Arg?Met?Ala?Leu?Thr?Pro?Ala?Glu?Ala?Phe?Val?Leu?Val?Arg?Thr?Ala

385?????????????????390?????????????????395?????????????????400

Ala?Leu?Lys?Leu?Arg?Asp?Val?Gly?Val?Gly?Val?Val?Leu?Pro?Pro?Ser

405?????????????????410?????????????????415

Leu?Ser?Gly?Gly?Leu?Ala?Ser?Arg?Leu?Gly?Leu?Ser?Ile?Glu?Ala?Asp

420?????????????????425?????????????????430

Leu?Pro?Glu?Arg?Ser?Arg?Gly?Phe?Ser?Leu?Gly?Glu?Ser?Leu?Gln?Trp

435?????????????????440?????????????????445

Ser?Trp?Glu?Leu?Met?Ile?Gly?Gly?Val?Thr?Leu?Thr?Leu?Arg?Asp?Leu

450?????????????????455?????????????????460

Glu?Arg?Leu?Ala?Gly?Lys?Arg?Ser?Pro?Leu?Val?Gln?His?Lys?Gly?Ala

465?????????????????470?????????????????475?????????????????480

Trp?Ile?Glu?Leu?Arg?Pro?Gly?Asp?Leu?Arg?Asn?Ala?Glu?Lys?Phe?Cys

485?????????????????490?????????????????495

Ala?Leu?Asp?Pro?Val?Leu?Ser?Leu?Asp?Asp?Ala?Leu?Arg?Leu?Thr?Gly

500?????????????????505?????????????????510

Asn?Glu?Gly?Glu?Thr?Leu?Gln?Arg?Leu?Pro?Val?His?Arg?Phe?Thr?Ala

515?????????????????520?????????????????525

Gly?Pro?Arg?Leu?Lys?Ala?Val?Leu?Glu?Gln?Tyr?His?Gln?Gln?Lys?Ala

530?????????????????535?????????????????540

Pro?Asp?Pro?Leu?Pro?Ala?Pro?Glu?Gly?Phe?Ala?Gly?Gln?Leu?Arg?Pro

545?????????????????550?????????????????555?????????????????560

Tyr?Gln?Glu?Arg?Gly?Leu?Gly?Trp?Leu?Ala?Phe?Leu?His?Arg?Phe?Asp

565?????????????????570?????????????????575

Gln?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln

580?????????????????585?????????????????590

Leu?Leu?Ala?Phe?Leu?Gln?His?Leu?Lys?Ala?Glu?Gln?Glu?Leu?Lys?Arg

595?????????????????600?????????????????605

Pro?Val?Leu?Leu?Val?Ala?Pro?Thr?Ser?Val?Leu?Thr?Asn?Trp?Leu?Arg

610?????????????????615?????????????????620

Glu?Ala?Lys?Ala?Phe?Thr?Pro?Glu?Leu?Asn?Val?Val?Glu?His?Tyr?Gly

625?????????????????630?????????????????635?????????????????640

Pro?Arg?Arg?Pro?Ser?Thr?Pro?Ala?Ala?Leu?Lys?Lys?Lys?Leu?Glu?Gly

645?????????????????650?????????????????655

Met?Asp?Leu?Val?Leu?Thr?Ser?Tyr?Gly?Leu?Leu?Gln?Arg?Asp?Ser?Glu

660?????????????????665?????????????????670

Leu?Leu?Ser?Ser?Leu?Asp?Trp?Gln?Gly?Val?Val?Ile?Asp?Glu?Ala?Gln

675?????????????????680?????????????????685

Ala?Ile?Lys?Asn?Ser?Ser?Ala?Arg?Gln?Ser?Gln?Ala?Ala?Arg?Asp?Leu

690?????????????????695?????????????????700

Ala?Arg?Pro?Leu?Lys?Gln?Ser?Arg?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr

705?????????????????710?????????????????715?????????????????720

Pro?Val?Glu?Asn?Arg?Val?Ser?Glu?Leu?Trp?Ala?Leu?Met?Asp?Phe?Leu

725?????????????????730?????????????????735

Asn?Pro?Lys?Val?Leu?Gly?Glu?Glu?Glu?Phe?Phe?Arg?Gln?Arg?Tyr?Arg

740?????????????????745?????????????????750

Leu?Pro?Ile?Glu?Arg?Tyr?Gly?Asp?Met?Ala?Ser?Val?Arg?Asp?Leu?Lys

755?????????????????760?????????????????765

Ala?Arg?Val?Gly?Pro?Phe?Ile?Leu?Arg?Arg?Leu?Lys?Thr?Asp?Arg?Ser

770?????????????????775?????????????????780

Ile?Ile?Ser?Asp?Leu?Pro?Glu?Lys?Val?Glu?Leu?Lys?Glu?Trp?Val?Gly

785?????????????????790?????????????????795?????????????????800

Leu?Ser?Pro?Glu?Gln?Val?Lys?Leu?Tyr?Arg?Arg?Thr?Val?Glu?Asp?Thr

805?????????????????810?????????????????815

Leu?Asp?Ala?Ile?Ala?Arg?Ala?Pro?Val?Gly?Gln?Lys?His?Gly?Gln?Val

820?????????????????825?????????????????830

Leu?Gly?Leu?Leu?Thr?Lys?Leu?Lys?Gln?Val?Cys?Asn?His?Pro?Ala?Leu

835?????????????????840?????????????????845

Met?Leu?Lys?Glu?Gly?Glu?Val?Gly?Ala?Gly?Phe?Ser?Ala?Arg?Ser?Ala

850?????????????????855?????????????????860

Lys?Leu?Gln?Arg?Leu?Glu?Glu?Ile?Val?Glu?Glu?Val?Ile?Ala?Ala?Gly

865?????????????????870?????????????????875?????????????????880

Asp?Arg?Ala?Leu?Leu?Phe?Thr?Gln?Phe?Ala?Glu?Trp?Gly?His?Leu?Leu

885?????????????????890?????????????????895

Gln?Thr?His?Leu?Gln?Gln?Arg?Phe?His?Gln?Glu?Val?Pro?Phe?Leu?Tyr

900?????????????????905?????????????????910

Gly?Ser?Thr?Ser?Lys?Gly?Glu?Arg?Gln?Ala?Met?Val?Asp?Arg?Phe?Gln

915?????????????????920?????????????????925

Asp?Asp?Pro?Arg?Gly?Pro?Gln?Leu?Phe?Leu?Leu?Ser?Leu?Lys?Ala?Gly

930?????????????????935?????????????????940

Gly?Val?Gly?Leu?Asn?Leu?Thr?Arg?Ala?Ser?His?Val?Phe?His?Ile?Asp

945?????????????????950?????????????????955?????????????????960

Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp?Arg?Ala?Tyr

965?????????????????970?????????????????975

Arg?Ile?Gly?Gln?Thr?Asn?Arg?Val?Met?Val?His?Lys?Phe?Ile?Thr?Ser

980?????????????????985?????????????????990

Gly?Ser?Val?Glu?Glu?Lys?Ile?Asp??Arg?Met?Ile?Arg?Glu??Lys?Ala?Arg

995?????????????????1000?????????????????1005

Leu?Ala??Glu?Asp?Ile?Val?Gly??Ser?Gly?Glu?Glu?Trp??Leu?Gly?Gly

1010?????????????????1015?????????????????1020

Leu?Asp??Pro?Gly?Gln?Leu?Arg??Asp?Leu?Val?Ala?Leu??Glu?Glu

1025?????????????????1030?????????????????1035

<210>83

<211>3090

<212>DNA

＜213〉synechococcus

<400>83

atgagcctgc?tgcacgccac?ctggcttccc?gccattcgta?cttccagcag?ttccggacaa?????60

ccggcactgc?tcgtttgggc?tgacacctgg?cgtgtcgcct?caccggaggg?acctggactc????120

acacccgctc?tgcatccctt?cacccttggc?tcgaacgatc?tcaaggcttg?gttgaccgaa????180

cgggacctga?tgcctggggg?cagcatcgat?gccaccgcct?gcctcaccct?cccaagccgc????240

accgtcaaac?cccgcaaaag?tcgaacccaa?tcgagcgaac?cagatccgga?ggggccagcc????300

tggaccgggt?tgccaatgca?agcgggagaa?cccattccaa?aacaaatgga?atggtggcca????360

tggcaagtgc?aaggcctggc?ggtcgagcca?tcggccgcca?cggaatggct?ggcccgttta????420

cccctatcgg?gccgacatcc?agaccttggg?gatgaactgc?gctggtggag?tcacctccaa????480

cgttggtccc?tcagcttggt?ggcccgtggt?cgctggattc?cccaaatgga?attaagcaaa????540

ggcgaggggt?acccccaccg?agcgcgctgg?gttcccctgc?tgaaccgtga?ggaggatcga????600

cgccggctcg?aagacctcgc?cgcgacgctg?cccctcgtag?cgacctgtgc?cctcccttgg????660

cgtgagccac?tcggacgccg?cagcaaccgc?accaccaggc?ttcgaccgga?agcgatgcga????720

gccgccaatc?cggtcgcctg?ctgtcgccca?cgaagcggtc?gcctcagggt?ggccaccttg????780

cttgaagact?tggtggatgc?ggagctgcgc?aagggatttg?aaccaagcac?ggaaggcctc????840

gaccccttac?tcaccttgtg?gcaagaggcc?ctggcctcag?aaaccggtgt?tgtggaggtg????900

ggcaacgaag?acgcagaacg?cctcaccgcg?gcaagcctgc?actggcgcga?gggaattgcc????960

ggaggcttcg?cggccgcccg?cacctgcctc?gaactcaaca?ccccaaacga?aggcgaagaa???1020

ctctgggacc?tgaagtttgg?attgcaagcg?gaggccgatc?ccagcctcaa?gctgccggcc???1080

gccgcggcct?gggcctcagg?agcggaaacc?cttcaactgg?gggaaatcca?agttgaccag???1140

gcgggggaag?tgctgctgga?gggtcttggc?cgagccctca?cggtgttccc?tccgatcgaa???1200

cgcggactgg?aaagcgcaac?accggaaacg?atgcagctca?ctccagcgga?ggcatttgtg???1260

ttggtgcgaa?cagcaacgca?ccagctccgc?aatgccggca?tcggcgtcga?actgcccccc???1320

agtctttcag?ggggcctcgc?cagccggctt?ggcttagcga?ttaaagcgga?tctaccggat???1380

cgatccagcg?gcttcaccct?cggcgaatct?cttgactgga?gctgggatct?catgatcggc???1440

ggcgtcacac?tcaccctccg?agagctcgaa?cgtctcagcg?gtaagcgaag?tccgctggta???1500

cgccacaagg?gcgcctggat?cgaactacgg?cccaacgatc?tccgcaacgc?cgaacgcttt???1560

tgtggagcca?atccagaact?gagcctcgac?gacgcactac?ggctcacggc?cacagaaggg????1620

gagctcatga?tgcgcctgcc?ggtgcatcgc?tttgatgcag?ggcctcgtct?tcagggagtt????1680

ctcgagcaat?accaccagca?aaaagccccc?gatcccctgc?cagctccaga?gggattttcc????1740

ggacaactcc?gtccctatca?agaacgtggc?ttgggctggc?tggccttcct?gcatcgcttc????1800

gatcagggcg?cctgcctggc?ggacgacatg?ggcttgggca?agaccatcca?gttattggcg????1860

ttcctgcagc?acctcaaagc?ggaaaacgaa?ctcaaacgcc?cggtgctgtt?ggtggcccca????1920

acctcggtgc?tcacgaattg?gcgacgggaa?gcggaagcct?tcacccctga?gctgtcggtg????1980

agagagcact?acgggccacg?ccggccttcc?acgccggccg?ccttgaaaaa?agagctcaaa????2040

ggtgtggatc?tggtgctcac?cagttacgga?ctgatgcaac?gcgacagtga?gctgctggac????2100

aacctcgact?ggcaaggggt?tgtgatcgat?gaagctcagg?cgatcaagaa?ccctggggca????2160

aagcaaagcc?aagcggcccg?agacctagcg?cgagccggga?agagcagcag?gttccgcatt????2220

gcactcacgg?gcacaccggt?ggaaaaccgc?gtcagcgagc?tgtgggcgct?gatggatttc????2280

ctcaacccca?aagtgttggg?tgaggaagac?ttttttcgtc?agcgctaccg?catgccaatt????2340

gagcgctacg?gcgatatgtc?gtcgttacgc?gatctcaaag?cacgggttgg?tcccttcatc????2400

ctgcgccgcc?tcaaaaccga?caagtcgatc?atttccgacc?tgcctgaaaa?ggtggagctc????2460

agcgaatggg?tggggctcag?caaagaacag?aaatcgctgt?acaacaaaac?cgttgaagac????2520

accctcgatg?ccattgccac?cgcacctcga?gggcaacgcc?atggccaggt?gctggcgctc????2580

ttgacccgtt?taaaacagat?ttgcaatcac?ccggccttag?cccaacgcga?aggtgccgtt????2640

gacgccgaat?tccttagccg?gtccgccaag?ctcatgcggc?tggaagaaat?ccttgaagag????2700

gtgattgaag?ccggcgatcg?cgctttgctg?ttcacccagt?tcgccgaatg?gggacacctc????2760

ttgcaggcct?ggatgcaaca?acgctggaag?tctgaggttc?cctttctgca?cggcggaacc????2820

cgcaaaagtg?atcggcaagc?gatggtggat?cgattccaag?aggacccccg?gggacctcaa????2880

ctcttccttc?tctccctcaa?ggccggtggt?gttggcctaa?acctcacccg?ggccagccac??2940

gtgttccacg?ttggatcgct?ggtggaatcc?agcggtggaa?aaccaagcca?ccgaccgggc??3000

ctatcgaatt?ggtcaaacca?accgggtgat?ggtgcacaaa?ttcgtcaccc?gtggctcggt??3060

ggaagaaaaa?atcgaccaaa?tgattcgtga???????????????????????????????????3090

<210>84

<211>1029

<212>PRT

＜213〉synechococcus

<400>84

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Pro?Ala?Ile?Arg?Thr?Ser?Ser

1???????????????5???????????????????10??????????????????15

Ser?Ser?Gly?Gln?Pro?Ala?Leu?Leu?Val?Trp?Ala?Asp?Thr?Trp?Arg?Val

20??????????????????25??????????????????30

Ala?Ser?Pro?Glu?Gly?Pro?Gly?Leu?Thr?Pro?Ala?Leu?His?Pro?Phe?Thr

35??????????????????40??????????????????45

Leu?Gly?Ser?Asn?Asp?Leu?Lys?Ala?Trp?Leu?Thr?Glu?Arg?Asp?Leu?Met

50??????????????????55??????????????????60

Pro?Gly?Gly?Ser?Ile?Asp?Ala?Thr?Ala?Cys?Leu?Thr?Leu?Pro?Ser?Arg

65??????????????????70??????????????????75??????????????????80

Thr?Val?Lys?Pro?Arg?Lys?Ser?Arg?Thr?Gln?Ser?Ser?Glu?Pro?Asp?Pro

85??????????????????90??????????????????95

Glu?Gly?Pro?Ala?Trp?Thr?Gly?Leu?Pro?Met?Gln?Ala?Gly?Glu?Pro?Ile

100?????????????????105?????????????????110

Pro?Lys?Gln?Met?Glu?Trp?Trp?Pro?Trp?Gln?Val?Gln?Gly?Leu?Ala?Val

115?????????????????120?????????????????125

Glu?Pro?Ser?Ala?Ala?Thr?Glu?Trp?Leu?Ala?Arg?Leu?Pro?Leu?Ser?Gly

130?????????????????135?????????????????140

Arg?His?Pro?Asp?Leu?Gly?Asp?Glu?Leu?Arg?Trp?Trp?Ser?His?Leu?Gln

145?????????????????150?????????????????155?????????????????160

Arg?Trp?Ser?Leu?Ser?Leu?Val?Ala?Arg?Gly?Arg?Trp?Ile?Pro?Gln?Met

165?????????????????170?????????????????175

Glu?Leu?Ser?Lys?Gly?Glu?Gly?Tyr?Pro?His?Arg?Ala?Arg?Trp?Val?Pro

180?????????????????185?????????????????190

Leu?Leu?Asn?Arg?Glu?Glu?Asp?Arg?Arg?Arg?Leu?Glu?Asp?Leu?Ala?Ala

195?????????????????200?????????????????205

Thr?Leu?Pro?Leu?Val?Ala?Thr?Cys?Ala?Leu?Pro?Trp?Arg?Glu?Pro?Leu

210?????????????????215?????????????????220

Gly?Arg?Arg?Ser?Asn?Arg?Thr?Thr?Arg?Leu?Arg?Pro?Glu?Ala?Met?Arg

225?????????????????230?????????????????235?????????????????240

Ala?Ala?Asn?Pro?Val?Ala?Cys?Cys?Arg?Pro?Arg?Ser?Gly?Arg?Leu?Arg

245?????????????????250?????????????????255

Val?Ala?Thr?Leu?Leu?Glu?Asp?Leu?Val?Asp?Ala?Glu?Leu?Arg?Lys?Gly

260?????????????????265?????????????????270

Phe?Glu?Pro?Ser?Thr?Glu?Gly?Leu?Asp?Pro?Leu?Leu?Thr?Leu?Trp?Gln

275?????????????????280?????????????????285

Glu?Ala?Leu?Ala?Ser?Glu?Thr?Gly?Val?Val?Glu?Val?Gly?Asn?Glu?Asp

290?????????????????295?????????????????300

Ala?Glu?Arg?Leu?Thr?Ala?Ala?Ser?Leu?His?Trp?Arg?Glu?Gly?Ile?Ala

305?????????????????310?????????????????315?????????????????320

Gly?Gly?Phe?Ala?Ala?Ala?Arg?Thr?Cys?Leu?Glu?Leu?Asn?Thr?Pro?Asn

325?????????????????330?????????????????335

Glu?Gly?Glu?Glu?Leu?Trp?Asp?Leu?Lys?Phe?Gly?Leu?Gln?Ala?Glu?Ala

340?????????????????345?????????????????350

Asp?Pro?Ser?Leu?Lys?Leu?Pro?Ala?Ala?Ala?Ala?Trp?Ala?Ser?Gly?Ala

355?????????????????360?????????????????365

Glu?Thr?Leu?Gln?Leu?Gly?Glu?Ile?Gln?Val?Asp?Gln?Ala?Gly?Glu?Val

370?????????????????375?????????????????380

Leu?Leu?Glu?Gly?Leu?Gly?Arg?Ala?Leu?Thr?Val?Phe?Pro?Pro?Ile?Glu

385?????????????????390?????????????????395?????????????????400

Arg?Gly?Leu?Glu?Ser?Ala?Thr?Pro?Glu?Thr?Met?Gln?Leu?Thr?Pro?Ala

405?????????????????410?????????????????415

Glu?Ala?Phe?Val?Leu?Val?Arg?Thr?Ala?Thr?His?Gln?Leu?Arg?Asn?Ala

420?????????????????425?????????????????430

Gly?Ile?Gly?Val?Glu?Leu?Pro?Pro?Ser?Leu?Ser?Gly?Gly?Leu?Ala?Ser

435?????????????????440?????????????????445

Arg?Leu?Gly?Leu?Ala?Ile?Lys?Ala?Asp?Leu?Pro?Asp?Arg?Ser?Ser?Gly

450?????????????????455?????????????????460

Phe?Thr?Leu?Gly?Glu?Ser?Leu?Asp?Trp?Ser?Trp?Asp?Leu?Met?Ile?Gly

465?????????????????470?????????????????475?????????????????480

Gly?Val?Thr?Leu?Thr?Leu?Arg?Glu?Leu?Glu?Arg?Leu?Ser?Gly?Lys?Arg

485?????????????????490?????????????????495

Ser?Pro?Leu?Val?Arg?His?Lys?Gly?Ala?Trp?Ile?Glu?Leu?Arg?Pro?Asn

500?????????????????505?????????????????510

Asp?Leu?Arg?Asn?Ala?Glu?Arg?Phe?Cys?Gly?Ala?Asn?Pro?Glu?Leu?Ser

515?????????????????520?????????????????525

Leu?Asp?Asp?Ala?Leu?Arg?Leu?Thr?Ala?Thr?Glu?Gly?Glu?Leu?Met?Met

530?????????????????535?????????????????540

Arg?Leu?Pro?Val?His?Arg?Phe?Asp?Ala?Gly?Pro?Arg?Leu?Gln?Gly?Val

545?????????????????550?????????????????555?????????????????560

Leu?Glu?Gln?Tyr?His?Gln?Gln?Lys?Ala?Pro?Asp?Pro?Leu?Pro?Ala?Pro

565?????????????????570?????????????????575

Glu?Gly?Phe?Ser?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Leu?Gly

580?????????????????585?????????????????590

Trp?Leu?Ala?Phe?Leu?His?Arg?Phe?Asp?Gln?Gly?Ala?Cys?Leu?Ala?Asp

595?????????????????600?????????????????605

Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala?Phe?Leu?Gln?His

610?????????????????615?????????????????620

Leu?Lys?Ala?Glu?Asn?Glu?Leu?Lys?Arg?Pro?Val?Leu?Leu?Val?Ala?Pro

625?????????????????630?????????????????635?????????????????640

Thr?Ser?Val?Leu?Thr?Asn?Trp?Arg?Arg?Glu?Ala?Glu?Ala?Phe?Thr?Pro

645?????????????????650?????????????????655

Glu?Leu?Ser?Val?Arg?Glu?His?Tyr?Gly?Pro?Arg?Arg?Pro?Ser?Thr?Pro

660?????????????????665?????????????????670

Ala?Ala?Leu?Lys?Lys?Glu?Leu?Lys?Gly?Val?Asp?Leu?Val?Leu?Thr?Ser

675?????????????????680?????????????????685

Tyr?Gly?Leu?Met?Gln?Arg?Asp?Ser?Glu?Leu?Leu?Asp?Asn?Leu?Asp?Trp

690?????????????????695?????????????????700

Gln?Gly?Val?Val?Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys?Asn?Pro?Gly?Ala

705?????????????????710?????????????????715?????????????????720

Lys?Gln?Ser?Gln?Ala?Ala?Arg?Asp?Leu?Ala?Arg?Ala?Gly?Lys?Ser?Ser

725?????????????????730?????????????????735

Arg?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Val?Ser

740?????????????????745?????????????????750

Glu?Leu?Trp?Ala?Leu?Met?Asp?Phe?Leu?Asn?Pro?Lys?Val?Leu?Gly?Glu

755?????????????????760?????????????????765

Glu?Asp?Phe?Phe?Arg?Gln?Arg?Tyr?Arg?Met?Pro?Ile?Glu?Arg?Tyr?Gly

770?????????????????775?????????????????780

Asp?Met?Ser?Ser?Leu?Arg?Asp?Leu?Lys?Ala?Arg?Val?Gly?Pro?Phe?Ile

785?????????????????790?????????????????795?????????????????800

Leu?Arg?Arg?Leu?Lys?Thr?Asp?Lys?Ser?Ile?Ile?Ser?Asp?Leu?Pro?Glu

805?????????????????810?????????????????815

Lys?Val?Glu?Leu?Ser?Glu?Trp?Val?Gly?Leu?Ser?Lys?Glu?Gln?Lys?Ser

820?????????????????825?????????????????830

Leu?Tyr?Asn?Lys?Thr?Val?Glu?Asp?Thr?Leu?Asp?Ala?Ile?Ala?Thr?Ala

835?????????????????840?????????????????845

Pro?Arg?Gly?Gln?Arg?His?Gly?Gln?Val?Leu?Ala?Leu?Leu?Thr?Arg?Leu

850?????????????????855?????????????????860

Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Leu?Ala?Gln?Arg?Glu?Gly?Ala?Val

865?????????????????870?????????????????875?????????????????880

Asp?Ala?Glu?Phe?Leu?Ser?Arg?Ser?Ala?Lys?Leu?Met?Arg?Leu?Glu?Glu

885?????????????????890?????????????????895

Ile?Leu?Glu?Glu?Val?Ile?Glu?Ala?Gly?Asp?Arg?Ala?Leu?Leu?Phe?Thr

900?????????????????905?????????????????910

Gln?Phe?Ala?Glu?Trp?Gly?His?Leu?Leu?Gln?Ala?Trp?Met?Gln?Gln?Arg

915?????????????????920?????????????????925

Trp?Lys?Ser?Glu?Val?Pro?Phe?Leu?His?Gly?Gly?Thr?Arg?Lys?Ser?Asp

930?????????????????935?????????????????940

Arg?Gln?Ala?Met?Val?Asp?Arg?Phe?Gln?Glu?Asp?Pro?Arg?Gly?Pro?Gln

945?????????????????950?????????????????955?????????????????960

Leu?Phe?Leu?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr

965?????????????????970?????????????????975

Arg?Ala?Ser?His?Val?Phe?His?Val?Gly?Ser?Leu?Val?Glu?Ser?Ser?Gly

980?????????????????985?????????????????990

Gly?Lys?Pro?Ser?His?Arg?Pro?Gly??Leu?Ser?Asn?Trp?Ser??Asn?Gln?Pro

995?????????????????1000?????????????????1005

Gly?Asp??Gly?Ala?Gln?Ile?Arg??His?Pro?Trp?Leu?Gly??Gly?Arg?Lys

1010?????????????????1015?????????????????1020

Asn?Arg??Pro?Asn?Asp?Ser

1025

<210>85

<211>3195

<212>DNA

＜213〉synechococcus

<400>85

atgagcctgc?tgcacgccac?ctggcttccg?gccattcgta?ctcctaccag?ctctggacga?????60

gctgcccttt?tggtgtgggc?cgacacctgg?cgcgttgccg?agcctgcagg?cccaagtaca????120

acccctgcgc?ttcacccgtt?caccctcagc?ccagacgatc?tccgggcctt?gctcacggaa????180

cgggatcttt?tacccgacgg?catcattgat?gccacggcat?gcctcaccct?gccgagccgc????240

agcgtgaagc?cccgaaaaaa?acgcgaaaca?gagaccagca?gcactgaaca?gcccagctgg????300

acaggccttc?ccttacaggc?tggagaaccg?atccccaaac?aaacagagtg?gtggccttgg????360

caggttcagg?ggctcgcaat?tgaccccatg?gcggccaccg?cctggctgtc?caaactgcct????420

ctgtcaggac?gacatcctga?tttggctgat?gagttgcgct?ggtggagtca?catgcagcgt????480

tggtccctca?gcctcgtagc?ccgaagtcgc?tggctccccc?aagtggagct?gagcaagggc????540

gagggctatc?cccatcgcgc?ccgctgggta?ccgcttctga?atcgggaaga?agacaggcgc????600

cgtctagaag?acttggccgc?agggctccct?ctcgttgcca?cctgtgccct?gccttggcga????660

gaaccaacgg?gcaaacgcag?caaccgaatc?accaggctca?gaccagaagc?catgcgcgcc????720

gcgaatcccg?tggcttgctg?caggcctcgc?agcggacgac?taagggttgc?cacgttattg????780

gccgacctga?tggacgcgca?gctgcgcaag?ggctttactc?ctgaccctga?cggcttggac?????840

cccctgctac?gcgcctggga?ggaggccttg?agctcggata?caggtgaaat?ccaactcagc?????900

gatgaagaaa?ccgaacgcct?agccaccgcc?agtaatcatt?ggcgtgaagg?ggtcgctgga?????960

aatgttgctg?cagcccgcgc?ctgcctggag?ctggcaacac?cagcggacga?tgaggacctt????1020

tggccactgc?gcttctttct?gcaggcggaa?gcagatccaa?ccctcaagct?gcccgcagga????1080

gcggcatggg?ctgcaggccc?cagcggcctc?caacttgggg?aaatcaaggt?ggagcacccc????1140

agcgaggtct?tgctcgaggg?tatggggcga?gccctgaccg?tgttccaacc?gatcgagcgc????1200

ggactggaca?gtgccacgcc?agagagcatg?cagctcacac?cagctgaagc?gtttgttttg????1260

gtgcgcacag?cagtccgaca?actgcgggat?gtgggcgttg?gcgttgacct?gccaccaagc????1320

ctgtctggag?ggctggctag?caggcttggc?ctcgccatca?aggcagaact?ctccgagcgt????1380

tcgcgaggct?tcacgctcgg?tgaaaacctt?gactggagct?gggagctgat?gatcggcggg????1440

gtgacgctga?ccttgcgaga?gcttgagcga?ttggctggta?agcgcagccc?tctggtgcgt????1500

cacaaagggg?cttggatcga?actacggccc?aatgacctca?aaaatgccga?gcgcttttgc????1560

gccgccaatc?cagacctgag?cctcgacgac?gcgcttcggc?tcaccgccac?cgaaggcgac????1620

acgatgatgc?gcctgcccgt?gcatcaattt?gatgccggtc?cgcggctgca?agccgtgctg????1680

gagcagtacc?accagcagaa?agcgccagac?ccactccccg?ctcccgaggg?cttttcgggt????1740

caactcaggc?cctatcaaga?gagaggactc?ggctggcttg?ccttcctgca?tcgcttcgac????1800

caaggcgcct?gcttggccga?tgacatgggc?cttggcaaaa?ccatccagct?gctggctttt????1860

ctgcaacacc?tcaaggcaga?aaacgaactc?aagcgatcag?tgcttttaat?tgcacccaca????1920

tctgtcctta?cgaactggaa?acgagaggca?acagcgttta?cacccgagct?caaggtgcat????1980

gagcactacg?gtccaaaacg?cccgagcacc?ccagcagcac?tgaaaaaggc?gctgaaagac????2040

gtggatctcg?tgctcaccag?ctatggcctg?ttacaacgcg?acagtgagct?cctcgaaagt????2100

cacgattggc?aaggcctcgt?gatcgatgaa?gcgcaggcga?taaaaaaccc?ctccgcgaag??2160

caaagccaag?ccgcccgtga?tctggcccgc?ccgaaaaaga?acagccgttt?tcgcatcgca??2220

ctcaccggca?caccagttga?gaaccgcgtc?agcgagctct?gggccctgat?ggacttcctc??2280

aaccctcggg?tactgggaga?ggaagaattt?ttccgacatc?gctatcgcat?gccgattgag??2340

cgttacggag?acctgtcctc?gctgcgcgac?ctcaaagccc?gagtgggacc?tttcatcctc??2400

agacgactca?aaacagacaa?agcgatcatc?tcggatctac?ccgagaaggt?ggaattgagc??2460

gagtgggttg?ggctgagcaa?agagcagaag?tcgctgtatg?ccaaaaccgt?tgaagacacc??2520

ttggatgcca?ttgcccgcgc?gccacgcggc?aaacgtcatg?gtcaggtgtt?gggtctgctc??2580

accaagctca?agcagatttg?caaccaccct?gcgcttgccc?tcaaggagca?gggcgccagc??2640

gaagatttcc?tcaaacggtc?cgtgaagctg?caacgtctcg?aagaaatttt?ggacgaggtt??2700

gtagaagctg?gggatcgagc?cttgctgttt?acccagttcg?cggaatgggg?caagttgctc??2760

caggattatt?tgcaacgacg?ctggcgcagc?gaagttccct?tcctcagcgg?cagcaccagc??2820

aaaagtgaac?ggcaagccat?ggtcgatcgc?ttccaggagg?atccgcgcgg?gccccagctt??2880

ttcctgttat?cactcaaagc?tggcggagtc?ggcctcaacc?tcacgcgcgc?cagtcatgtc??2940

tttcacatcg?accgttggtg?gaaccccgcc?gttgaaaatc?aagccacgga?ccgtgcctat??3000

cgcatcggcc?aaacgaaccg?ggtcatggtg?cataagttca?tcaccagcgg?ctccgttgag??3060

gagaaaattg?accgcatgat?ccgcgagaag?tccagactgg?cggaagacat?cattggctcc??3120

ggcgaagact?ggcttggagg?cctggaaatg?ggacaactca?aagagctagt?gagcctggag??3180

gacaaccaag?catga???????????????????????????????????????????????????3195

<210>86

<211>1064

<212>PRT

＜213〉synechococcus

<400>86

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Pro?Ala?Ile?Arg?Thr?Pro?Thr

1???????????????5???????????????????10??????????????????15

Ser?Ser?Gly?Arg?Ala?Ala?Leu?Leu?Val?Trp?Ala?Asp?Thr?Trp?Arg?Val

20??????????????????25??????????????????30

Ala?Glu?Pro?Ala?Gly?Pro?Ser?Thr?Thr?Pro?Ala?Leu?His?Pro?Phe?Thr

35??????????????????40??????????????????45

Leu?Ser?Pro?Asp?Asp?Leu?Arg?Ala?Leu?Leu?Thr?Glu?Arg?Asp?Leu?Leu

50??????????????????55??????????????????60

Pro?Asp?Gly?Ile?Ile?Asp?Ala?Thr?Ala?Cys?Leu?Thr?Leu?Pro?Ser?Arg

65??????????????????70??????????????????75??????????????????80

Ser?Val?Lys?Pro?Arg?Lys?Lys?Arg?Glu?Thr?Glu?Thr?Ser?Ser?Thr?Glu

85??????????????????90??????????????????95

Gln?Pro?Ser?Trp?Thr?Gly?Leu?Pro?Leu?Gln?Ala?Gly?Glu?Pro?Ile?Pro

100?????????????????105?????????????????110

Lys?Gln?Thr?Glu?Trp?Trp?Pro?Trp?Gln?Val?Gln?Gly?Leu?Ala?Ile?Asp

115?????????????????120?????????????????125

Pro?Met?Ala?Ala?Thr?Ala?Trp?Leu?Ser?Lys?Leu?Pro?Leu?Ser?Gly?Arg

130?????????????????135?????????????????140

His?Pro?Asp?Leu?Ala?Asp?Glu?Leu?Arg?Trp?Trp?Ser?His?Met?Gln?Arg

145?????????????????150?????????????????155?????????????????160

Trp?Ser?Leu?Ser?Leu?Val?Ala?Arg?Ser?Arg?Trp?Leu?Pro?Gln?Val?Glu

165?????????????????170?????????????????175

Leu?Ser?Lys?Gly?Glu?Gly?Tyr?Pro?His?Arg?Ala?Arg?Trp?Val?Pro?Leu

180?????????????????185?????????????????190

Leu?Asn?Arg?Glu?Glu?Asp?Arg?Arg?Arg?Leu?Glu?Asp?Leu?Ala?Ala?Gly

195?????????????????200?????????????????205

Leu?Pro?Leu?Val?Ala?Thr?Cys?Ala?Leu?Pro?Trp?Arg?Glu?Pro?Thr?Gly

210?????????????????215?????????????????220

Lys?Arg?Ser?Asn?Arg?Ile?Thr?Arg?Leu?Arg?Pro?Glu?Ala?Met?Arg?Ala

225?????????????????230?????????????????235?????????????????240

Ala?Asn?Pro?Val?Ala?Cys?Cys?Arg?Pro?Arg?Ser?Gly?Arg?Leu?Arg?Val

245?????????????????250?????????????????255

Ala?Thr?Leu?Leu?Ala?Asp?Leu?Met?Asp?Ala?Gln?Leu?Arg?Lys?Gly?Phe

260?????????????????265?????????????????270

Thr?Pro?Asp?Pro?Asp?Gly?Leu?Asp?Pro?Leu?Leu?Arg?Ala?Trp?Glu?Glu

275?????????????????280?????????????????285

Ala?Leu?Ser?Ser?Asp?Thr?Gly?Glu?Ile?Gln?Leu?Ser?Asp?Glu?Glu?Thr

290?????????????????295?????????????????300

Glu?Arg?Leu?Ala?Thr?Ala?Ser?Asn?His?Trp?Arg?Glu?Gly?Val?Ala?Gly

305?????????????????310?????????????????315?????????????????320

Asn?Val?Ala?Ala?Ala?Arg?Ala?Cys?Leu?Glu?Leu?Ala?Thr?Pro?Ala?Asp

325?????????????????330?????????????????335

Asp?Glu?Asp?Leu?Trp?Pro?Leu?Arg?Phe?Phe?Leu?Gln?Ala?Glu?Ala?Asp

340?????????????????345?????????????????350

Pro?Thr?Leu?Lys?Leu?Pro?Ala?Gly?Ala?Ala?Trp?Ala?Ala?Gly?Pro?Ser

355?????????????????360?????????????????365

Gly?Leu?Gln?Leu?Gly?Glu?Ile?Lys?Val?Glu?His?Pro?Ser?Glu?Val?Leu

370?????????????????375?????????????????380

Leu?Glu?Gly?Met?Gly?Arg?Ala?Leu?Thr?Val?Phe?Gln?Pro?Ile?Glu?Arg

385?????????????????390?????????????????395?????????????????400

Gly?Leu?Asp?Ser?Ala?Thr?Pro?Glu?Ser?Met?Gln?Leu?Thr?Pro?Ala?Glu

405?????????????????410?????????????????415

Ala?Phe?Val?Leu?Val?Arg?Thr?Ala?Val?Arg?Gln?Leu?Arg?Asp?Val?Gly

420?????????????????425?????????????????430

Val?Gly?Val?Asp?Leu?Pro?Pro?Ser?Leu?Ser?Gly?Gly?Leu?Ala?Ser?Arg

435?????????????????440?????????????????445

Leu?Gly?Leu?Ala?Ile?Lys?Ala?Glu?Leu?Ser?Glu?Arg?Ser?Arg?Gly?Phe

450?????????????????455?????????????????460

Thr?Leu?Gly?Glu?Asn?Leu?Asp?Trp?Ser?Trp?Glu?Leu?Met?Ile?Gly?Gly

465?????????????????470?????????????????475?????????????????480

Val?Thr?Leu?Thr?Leu?Arg?Glu?Leu?Glu?Arg?Leu?Ala?Gly?Lys?Arg?Ser

485?????????????????490?????????????????495

Pro?Leu?Val?Arg?His?Lys?Gly?Ala?Trp?Ile?Glu?Leu?Arg?Pro?Asn?Asp

500?????????????????505?????????????????510

Leu?Lys?Asn?Ala?Glu?Arg?Phe?Cys?Ala?Ala?Asn?Pro?Asp?Leu?Ser?Leu

515?????????????????520?????????????????525

Asp?Asp?Ala?Leu?Arg?Leu?Thr?Ala?Thr?Glu?Gly?Asp?Thr?Met?Met?Arg

530?????????????????535?????????????????540

Leu?Pro?Val?His?Gln?Phe?Asp?Ala?Gly?Pro?Arg?Leu?Gln?Ala?Val?Leu

545?????????????????550?????????????????555?????????????????560

Glu?Gln?Tyr?His?Gln?Gln?Lys?Ala?Pro?Asp?Pro?Leu?Pro?Ala?Pro?Glu

565?????????????????570?????????????????575

Gly?Phe?Ser?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Leu?Gly?Trp

580?????????????????585?????????????????590

Leu?Ala?Phe?Leu?His?Arg?Phe?Asp?Gln?Gly?Ala?Cys?Leu?Ala?Asp?Asp

595?????????????????600?????????????????605

Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala?Phe?Leu?Gln?His?Leu

610?????????????????615?????????????????620

Lys?Ala?Glu?Asn?Glu?Leu?Lys?Arg?Ser?Val?Leu?Leu?Ile?Ala?Pro?Thr

625?????????????????630?????????????????635?????????????????640

Ser?Val?Leu?Thr?Asn?Trp?Lys?Arg?Glu?Ala?Thr?Ala?Phe?Thr?Pro?Glu

645?????????????????650?????????????????655

Leu?Lys?Val?His?Glu?His?Tyr?Gly?Pro?Lys?Arg?Pro?Ser?Thr?Pro?Ala

660?????????????????665?????????????????670

Ala?Leu?Lys?Lys?Ala?Leu?Lys?Asp?Val?Asp?Leu?Val?Leu?Thr?Ser?Tyr

675?????????????????680?????????????????685

Gly?Leu?Leu?Gln?Arg?Asp?Ser?Glu?Leu?Leu?Glu?Ser?His?Asp?Trp?Gln

690?????????????????695?????????????????700

Gly?Leu?Val?Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys?Asn?Pro?Ser?Ala?Lys

705?????????????????710?????????????????715?????????????????720

Gln?Ser?Gln?Ala?Ala?Arg?Asp?Leu?Ala?Arg?Pro?Lys?Lys?Asn?Ser?Arg

725?????????????????730?????????????????735

Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Val?Ser?Glu

740?????????????????745?????????????????750

Leu?Trp?Ala?Leu?Met?Asp?Phe?Leu?Asn?Pro?Arg?Val?Leu?Gly?Glu?Glu

755?????????????????760?????????????????765

Glu?Phe?Phe?Arg?His?Arg?Tyr?Arg?Met?Pro?Ile?Glu?Arg?Tyr?Gly?Asp

770?????????????????775?????????????????780

Leu?Ser?Ser?Leu?Arg?Asp?Leu?Lys?Ala?Arg?Val?Gly?Pro?Phe?Ile?Leu

785?????????????????790?????????????????795?????????????????800

Arg?Arg?Leu?Lys?Thr?Asp?Lys?Ala?Ile?Ile?Ser?Asp?Leu?Pro?Glu?Lys

805?????????????????810?????????????????815

Val?Glu?Leu?Ser?Glu?Trp?Val?Gly?Leu?Ser?Lys?Glu?Gln?Lys?Ser?Leu

820?????????????????825?????????????????830

Tyr?Ala?Lys?Thr?Val?Glu?Asp?Thr?Leu?Asp?Ala?Ile?Ala?Arg?Ala?Pro

835?????????????????840?????????????????845

Arg?Gly?Lys?Arg?His?Gly?Gln?Val?Leu?Gly?Leu?Leu?Thr?Lys?Leu?Lys

850?????????????????855?????????????????860

Gln?Ile?Cys?Asn?His?Pro?Ala?Leu?Ala?Leu?Lys?Glu?Gln?Gly?Ala?Ser

865?????????????????870?????????????????875?????????????????880

Glu?Asp?Phe?Leu?Lys?Arg?Ser?Val?Lys?Leu?Gln?Arg?Leu?Glu?Glu?Ile

885?????????????????890?????????????????895

Leu?Asp?Glu?Val?Val?Glu?Ala?Gly?Asp?Arg?Ala?Leu?Leu?Phe?Thr?Gln

900?????????????????905?????????????????910

Phe?Ala?Glu?Trp?Gly?Lys?Leu?Leu?Gln?Asp?Tyr?Leu?Gln?Arg?Arg?Trp

915?????????????????920?????????????????925

Arg?Ser?Glu?Val?Pro?Phe?Leu?Ser?Gly?Ser?Thr?Ser?Lys?Ser?Glu?Arg

930?????????????????935?????????????????940

Gln?Ala?Met?Val?Asp?Arg?Phe?Gln?Glu?Asp?Pro?Arg?Gly?Pro?Gln?Leu

945?????????????????950?????????????????955?????????????????960

Phe?Leu?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr?Arg

965?????????????????970?????????????????975

Ala?Ser?His?Val?Phe?His?Ile?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu

980?????????????????985?????????????????990

Asn?Gln?Ala?Thr?Asp?Arg?Ala?Tyr??Arg?Ile?Gly?Gln?Thr??Asn?Arg?Val

995?????????????????1000?????????????????1005

Met?Val??His?Lys?Phe?Ile?Thr??Ser?Gly?Ser?Val?Glu??Glu?Lys?Ile

1010?????????????????1015?????????????????1020

Asp?Arg??Met?Ile?Arg?Glu?Lys??Ser?Arg?Leu?Ala?Glu??Asp?Ile?Ile

1025?????????????????1030?????????????????1035

Gly?Ser??Gly?Glu?Asp?Trp?Leu??Gly?Gly?Leu?Glu?Met??Gly?Gln?Leu

1040?????????????????1045?????????????????1050

Lys?Glu??Leu?Val?Ser?Leu?Glu??Asp?Asn?Gln?Ala

1055?????????????????1060

<210>87

<211>3198

<212>DNA

＜213〉synechococcus

<400>87

atgagcctgc?tgcacgccac?ctggcttccc?gccatccgca?cctccagcag?ttccggtcaa???60

ccggcactgc?tcgtttgggc?tgacacctgg?cgggtggcca?caccggaagg?cccgggcctt??120

accccagcgc?tgcacccctt?caccctaagc?catgaagacc?tcagggcctg?gctgagcgaa??180

cgcgacctct?tgcccggcgg?ctgcatcgat?gccacggcgt?gcctcaccct?gccgagccgc??240

acggtgaagc?tgcgcaaaag?ccgcagcaca?aaagaggagc?caacaccgga?accaccgggt??300

tggaccgggc?taccgatgca?ggccggcgaa?ccgatcccca?agcaaaccga?atggtggccc??360

tggcaggtgc?aggggctcgc?ggtggaaccg?tcggcagcca?cggagtggct?gtcccgattg??420

ccgctctccg?gcaccaatcc?agacctggct?gatgaactgc?gctggtggag?ccatctgcag??480

cgctgggcct?tgagtctggt?ggcccggggc?cgctggattc?cccagatgga?gttcagcaaa??540

ggggagggct?atccccatcg?ggcccgttgg?gtgccgcttc?tcaaccggga?agaagaccgg??600

cgccggctgg?aggatctggc?ggccagcctg?ccgctggtgg?ccacctgcgc?cttgccctgg??660

cgggaacccc?tggggcgccg?cagcaaccgc?accacccggt?tacgaccgga?ggcgatgcga??720

gccgccaacc?ctgtggccag?ctgccggccc?cgcagcggac?gcctgcgggt?ggcgacgctg??780

ctggaagatc?tagtggacgc?gcagctgcgc?aaggactttg?aaccctccac?cgatgggctt??840

gatcccctgc?tgaccctctg?gcaggaggcc?ctggggtcgg?agaccggggt?gatcgagatc??900

ggcgatgaag?aggccgaacg?cctggccacc?gccagccatc?actggcggga?gggcatcgcc??960

ggcgattttg?ctgcggcccg?cacctgcctt?gaactgcaca?ccccaccgga?tggggaggat????1020

ctctgggagc?tgcgcttcgg?gctgcaggcg?gaagctgacc?ccagcctgaa?gctcccggcc????1080

gccgcggcct?gggcggctgg?tgcggaaccg?ctacagcttg?gagagatccg?ggtggaccaa????1140

ccgggtgaag?tgctgctgga?aggcatgggc?cgcgccctga?gcgtgtttcc?ggcaattgag????1200

cggggtctgg?agagcgccac?acctgaaacg?atgcagctca?ccccggccga?ggccttcgtg????1260

ctggtgcgca?cggccgcccg?gcagctgcgg?gatgccggcg?tgggagtgga?gctgccgccc????1320

agcctctccg?gtggcctggc?cagccgactg?ggcctgtcga?tcaaagcgga?actgcccgaa????1380

cgctcgagcg?gtttcacgtt?gggtgagtgt?ctggcctggg?agtgggatct?gatgatcggc????1440

ggggtgacgc?tcaccctgcg?ggaattggag?cgcctgagcg?gcaagcgcag?ccccctggtg????1500

cgccacaagg?gggcctggat?cgaactgcgg?cccaacgacc?tcaaaaatgc?cgaacgcttc????1560

tgtggggcga?aacctgaact?gagcctcgac?gacgcgctgc?ggctgacggg?gacggaaggg????1620

gaactgttga?tgcggatgcc?ggtgcaccgc?ttcgacgccg?gcccacggct?gcaatcggtg????1680

ttgcagcaat?accaccagca?gaaggccccc?gaccccttgc?cggccccgga?aggattcagc????1740

gggcagctgc?ggccttatca?ggagcggggc?ctcggctggc?tcgccttcct?gcaccgcttc????1800

gatcaagggg?cctgtctagc?tgacgacatg?ggcttgggca?aaaccattca?gttgctagcg????1860

ttcctgcagc?acctcaaagc?ggagcaagaa?ctgaaacgcc?cggtgctgct?ggtggccccc????1920

acatcggtgc?tcaccaactg?gcgacgggag?gcggaatcgt?tcactccaga?gttgaaggtc????1980

accgagcatt?acgggcctcg?ccggccctcc?acacccgccg?aactcaaaaa?agcgttgaag????2040

gaggtggatc?tggtgctcac?cagctacggg?ctgctgcagc?gtgacagcga?actgctggaa????2100

acccaggact?ggcagggggt?ggtgattgac?gaagcccagg?cgatcaagaa?ccctggcgcc????2160

aaacagagcc?aagccgcccg?ggatctggcc?cgcaccggcc?gcatcaagag?caaccgcttc????2220

cgcatcgcac?tcaccggcac?ccccgtggaa?aaccgggtga?gcgaactgtg?ggccttgatg????2280

gacttcctca?acccaaaggt?gcttggggaa?gaagacttct?tccgccagcg?ctatcggatg??2340

ccgattgagc?gctacggcga?catgtcgtcc?ctgcgggacc?tgaaaggccg?cgtgggtccg??2400

ttcatcctgc?gccggctgaa?aaccgacaag?acgatcattt?ccgacctgcc?tgaaaaggtg??2460

gagctgagcg?aatgggtggg?gctgagcaag?gagcagaaat?ctctgtacag?caagaccgtg??2520

gaagacaccc?tcgatgccat?tgcccgggcg?ccgcgcgggc?agcgccacgg?gcaggtgctg??2580

gccctgctca?cccggctgaa?acagatctgc?aaccatcccg?ccctggccct?gagcgaaggg??2640

gccgtggacg?atggcttcct?gggccgttcg?gccaagctgc?agcggctgga?ggagatcctc??2700

gatgaggtga?tcgaagcggg?cgatcgggcc?ctgctgttca?cccagttcgc?cgaatggggg??2760

catttgctaa?gggcctggat?gcagcagcgc?tggaaatcag?aagtgccctt?cctgcacggc??2820

ggcacccgca?agaacgaacg?ccaggcgatg?gtggatcgct?tccaggagga?tccccgcggt??2880

ccacagctgt?tcctgctctc?gctcaaggcc?ggtggtgtgg?gcctcaacct?cacgcgggcc??2940

agccatgtgt?tccacatcga?tcgctggtgg?aaccctgccg?tggaaaacca?ggccaccgac??3000

cgggcctatc?ggatcggcca?aacgaaccga?gtgatggttc?ataaattcat?caccagcggt??3060

tcggtggagg?aaaaaatcga?tcgcatgatc?cgcgagaaat?cacgcctggc?cgaagatgtg??3120

atcggctccg?gcgaagattg?gctgggaagc?ctcggtggcg?atcaattgcg?cgatctcgtt??3180

tctttggagg?acacctga????????????????????????????????????????????????3198

<210>88

<211>1065

<212>PRT

＜213〉synechococcus

<400>88

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Pro?Ala?Ile?Arg?Thr?Ser?Ser

1???????????????5???????????????????10??????????????????15

Ser?Ser?Gly?Gln?Pro?Ala?Leu?Leu?Val?Trp?Ala?Asp?Thr?Trp?Arg?Val

20??????????????????25??????????????????30

Ala?Thr?Pro?Glu?Gly?Pro?Gly?Leu?Thr?Pro?Ala?Leu?His?Pro?Phe?Thr

35??????????????????40??????????????????45

Leu?Ser?His?Glu?Asp?Leu?Arg?Ala?Trp?Leu?Ser?Glu?Arg?Asp?Leu?Leu

50??????????????????55??????????????????60

Pro?Gly?Gly?Cys?Ile?Asp?Ala?Thr?Ala?Cys?Leu?Thr?Leu?Pro?Ser?Arg

65??????????????????70??????????????????75??????????????????80

Thr?Val?Lys?Leu?Arg?Lys?Ser?Arg?Ser?Thr?Lys?Glu?Glu?Pro?Thr?Pro

85??????????????????90??????????????????95

Glu?Pro?Pro?Gly?Trp?Thr?Gly?Leu?Pro?Met?Gln?Ala?Gly?Glu?Pro?Ile

100?????????????????105?????????????????110

Pro?Lys?Gln?Thr?Glu?Trp?Trp?Pro?Trp?Gln?Val?Gln?Gly?Leu?Ala?Val

115?????????????????120?????????????????125

Glu?Pro?Ser?Ala?Ala?Thr?Glu?Trp?Leu?Ser?Arg?Leu?Pro?Leu?Ser?Gly

130?????????????????135?????????????????140

Thr?Asn?Pro?Asp?Leu?Ala?Asp?Glu?Leu?Arg?Trp?Trp?Ser?His?Leu?Gln

145?????????????????150?????????????????155?????????????????160

Arg?Trp?Ala?Leu?Ser?Leu?Val?Ala?Arg?Gly?Arg?Trp?Ile?Pro?Gln?Met

165?????????????????170?????????????????175

Glu?Phe?Ser?Lys?Gly?Glu?Gly?Tyr?Pro?His?Arg?Ala?Arg?Trp?Val?Pro

180?????????????????185?????????????????190

Leu?Leu?Asn?Arg?Glu?Glu?Asp?Arg?Arg?Arg?Leu?Glu?Asp?Leu?Ala?Ala

195?????????????????200?????????????????205

Ser?Leu?Pro?Leu?Val?Ala?Thr?Cys?Ala?Leu?Pro?Trp?Arg?Glu?Pro?Leu

210?????????????????215?????????????????220

Gly?Arg?Arg?Ser?Asn?Arg?Thr?Thr?Arg?Leu?Arg?Pro?Glu?Ala?Met?Arg

225?????????????????230?????????????????235?????????????????240

Ala?Ala?Asn?Pro?Val?Ala?Ser?Cys?Arg?Pro?Arg?Ser?Gly?Arg?Leu?Arg

245?????????????????250?????????????????255

Val?Ala?Thr?Leu?Leu?Glu?Asp?Leu?Val?Asp?Ala?Gln?Leu?Arg?Lys?Asp

260?????????????????265?????????????????270

Phe?Glu?Pro?Ser?Thr?Asp?Gly?Leu?Asp?Pro?Leu?Leu?Thr?Leu?Trp?Gln

275?????????????????280?????????????????285

Glu?Ala?Leu?Gly?Ser?Glu?Thr?Gly?ValIle?Glu?Ile?Gly?Asp?Glu?Glu

290?????????????????295?????????????????300

Ala?Glu?Arg?Leu?Ala?Thr?Ala?Ser?His?His?Trp?Arg?Glu?Gly?Ile?Ala

305?????????????????310?????????????????315?????????????????320

Gly?Asp?Phe?Ala?Ala?Ala?Arg?Thr?Cys?Leu?Glu?Leu?His?Thr?Pro?Pro

325?????????????????330?????????????????335

Asp?Gly?Glu?Asp?Leu?Trp?Glu?Leu?Arg?Phe?Gly?Leu?Gln?Ala?Glu?Ala

340?????????????????345?????????????????350

Asp?Pro?Ser?Leu?Lys?Leu?Pro?Ala?Ala?Ala?Ala?Trp?Ala?Ala?Gly?Ala

355?????????????????360?????????????????365

Glu?Pro?Leu?Gln?Leu?Gly?Glu?Ile?Arg?Val?Asp?Gln?Pro?Gly?Glu?Val

370?????????????????375?????????????????380

Leu?Leu?Glu?Gly?Met?Gly?Arg?Ala?Leu?Ser?Val?Phe?Pro?Ala?Ile?Glu

385?????????????????390?????????????????395?????????????????400

Arg?Gly?Leu?Glu?Ser?Ala?Thr?Pro?Glu?Thr?Met?Gln?Leu?Thr?Pro?Ala

405?????????????????410?????????????????415

Glu?Ala?Phe?Val?Leu?Val?Arg?Thr?Ala?Ala?Arg?Gln?Leu?Arg?Asp?Ala

420?????????????????425?????????????????430

Gly?Val?Gly?Val?Glu?Leu?Pro?Pro?Ser?Leu?Ser?Gly?Gly?Leu?Ala?Ser

435?????????????????440?????????????????445

Arg?Leu?Gly?Leu?Ser?Ile?Lys?Ala?Glu?Leu?Pro?Glu?Arg?Ser?Ser?Gly

450?????????????????455?????????????????460

Phe?Thr?Leu?Gly?Glu?Cys?Leu?Ala?Trp?Glu?Trp?Asp?Leu?Met?Ile?Gly

465?????????????????470?????????????????475?????????????????480

Gly?Val?Thr?Leu?Thr?Leu?Arg?Glu?Leu?Glu?Arg?Leu?Ser?Gly?Lys?Arg

485?????????????????490?????????????????495

Ser?Pro?Leu?Val?Arg?His?Lys?Gly?Ala?Trp?Ile?Glu?Leu?Arg?Pro?Asn

500?????????????????505?????????????????510

Asp?Leu?Lys?Asn?Ala?Glu?Arg?Phe?Cys?Gly?Ala?Lys?Pro?Glu?Leu?Ser

515?????????????????520?????????????????525

Leu?Asp?Asp?Ala?Leu?Arg?Leu?Thr?Gly?Thr?Glu?Gly?Glu?Leu?Leu?Met

530?????????????????535?????????????????540

Arg?Met?Pro?Val?His?Arg?Phe?Asp?Ala?Gly?Pro?Arg?Leu?Gln?Ser?Val

545?????????????????550?????????????????555?????????????????560

Leu?Gln?Gln?Tyr?His?Gln?Gln?Lys?Ala?Pro?Asp?Pro?Leu?Pro?Ala?Pro

565?????????????????570?????????????????575

Glu?Gly?Phe?Ser?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Leu?Gly

580?????????????????585?????????????????590

Trp?Leu?Ala?Phe?Leu?His?Arg?Phe?Asp?Gln?Gly?Ala?Cys?Leu?Ala?Asp

595?????????????????600?????????????????605

Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala?Phe?Leu?Gln?His

610?????????????????615?????????????????620

Leu?Lys?Ala?Glu?Gln?Glu?Leu?Lys?Arg?Pro?Val?Leu?Leu?Val?Ala?Pro

625?????????????????630?????????????????635?????????????????640

Thr?Ser?Val?Leu?Thr?Asn?Trp?Arg?Arg?Glu?Ala?Glu?Ser?Phe?Thr?Pro

645?????????????????650?????????????????655

Glu?Leu?Lys?Val?Thr?Glu?His?Tyr?Gly?Pro?Arg?Arg?Pro?Ser?Thr?Pro

660?????????????????665?????????????????670

Ala?Glu?Leu?Lys?Lys?Ala?Leu?Lys?Glu?Val?Asp?Leu?Val?Leu?Thr?Ser

675?????????????????680?????????????????685

Tyr?Gly?Leu?Leu?Gln?Arg?Asp?Ser?Glu?Leu?Leu?Glu?Thr?Gln?Asp?Trp

690?????????????????695?????????????????700

Gln?Gly?Val?Val?Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys?Asn?Pro?Gly?Ala

705?????????????????710?????????????????715?????????????????720

Lys?Gln?Ser?Gln?Ala?Ala?Arg?Asp?Leu?Ala?Arg?Thr?Gly?Arg?Ile?Lys

725?????????????????730?????????????????735

Ser?Asn?Arg?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg

740?????????????????745?????????????????750

Val?Ser?Glu?Leu?Trp?Ala?Leu?Met?Asp?Phe?Leu?Asn?Pro?Lys?Val?Leu

755?????????????????760?????????????????765

Gly?Glu?Glu?Asp?Phe?Phe?Arg?Gln?Arg?Tyr?Arg?Met?Pro?Ile?Glu?Arg

770?????????????????775?????????????????780

Tyr?Gly?Asp?Met?Ser?Ser?Leu?Arg?Asp?Leu?Lys?Gly?Arg?Val?Gly?Pro

785?????????????????790?????????????????795?????????????????800

Phe?Ile?Leu?Arg?Arg?Leu?Lys?Thr?Asp?Lys?Thr?Ile?Ile?Ser?Asp?Leu

805?????????????????810?????????????????815

Pro?Glu?Lys?Val?Glu?Leu?Ser?Glu?Trp?Val?Gly?Leu?Ser?Lys?Glu?Gln

820?????????????????825?????????????????830

Lys?Ser?Leu?Tyr?Ser?Lys?Thr?Val?Glu?Asp?Thr?Leu?Asp?Ala?Ile?Ala

835?????????????????840?????????????????845

Arg?Ala?Pro?Arg?Gly?Gln?Arg?His?Gly?Gln?Val?Leu?Ala?Leu?Leu?Thr

850?????????????????855?????????????????860

Arg?Leu?Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Leu?Ala?Leu?Ser?Glu?Gly

865?????????????????870?????????????????875?????????????????880

Ala?Val?Asp?Asp?Gly?Phe?Leu?Gly?Arg?Ser?Ala?Lys?Leu?Gln?Arg?Leu

885?????????????????890?????????????????895

Glu?Glu?Ile?Leu?Asp?Glu?Val?Ile?Glu?Ala?Gly?Asp?Arg?Ala?Leu?Leu

900?????????????????905?????????????????910

Phe?Thr?Gln?Phe?Ala?Glu?Trp?Gly?His?Leu?Leu?Arg?Ala?Trp?Met?Gln

915?????????????????920?????????????????925

Gln?Arg?Trp?Lys?Ser?Glu?Val?Pro?Phe?Leu?His?Gly?Gly?Thr?Arg?Lys

930?????????????????935?????????????????940

Asn?Glu?Arg?Gln?Ala?Met?Val?Asp?Arg?Phe?Gln?Glu?Asp?Pro?Arg?Gly

945?????????????????950?????????????????955?????????????????960

Pro?Gln?Leu?Phe?Leu?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn

965?????????????????970?????????????????975

Leu?Thr?Arg?Ala?Ser?His?Val?Phe?His?Ile?Asp?Arg?Trp?Trp?Asn?Pro

980?????????????????985?????????????????990

Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp??Arg?Ala?Tyr?Arg?Ile??Gly?Gln?Thr

995?????????????????1000?????????????????1005

Asn?Arg??Val?Met?Val?His?Lys??Phe?Ile?Thr?Ser?Gly??Ser?Val?Glu

1010?????????????????1015?????????????????1020

Glu?Lys??Ile?Asp?Arg?Met?Ile??Arg?Glu?Lys?Ser?Arg??Leu?Ala?Glu

1025?????????????????1030?????????????????1035

Asp?Val???Ile?Gly?Ser?Gly?Glu??Asp?Trp?Leu?Gly?Ser??Leu?Gly?Gly

1040?????????????????1045?????????????????1050

Asp?Gln??Leu?Arg?Asp?Leu?Val??Ser?Leu?Glu?Asp?Thr

1055?????????????????1060?????????????????1065

<210>89

<211>3192

<212>DNA

＜213〉synechococcus

<400>89

atgagcctgc?tgcacgccac?ctggcttccc?gccattcgta?cttccagcag?ttccggacag???60

ccggcactgc?tcatttgggc?tgacacctgg?cgtgtcgcct?caccggaggg?gcccggactc??120

acacccgctc?tgcatccctt?cacccttggc?tcggacgatc?tcaaagcttg?gttgaccgaa??180

cgggacctga?tgcctggggg?cagcatcgat?gccaccgcct?gcctcaccct?cccaagccgc??240

agcgtcaaac?cccgcaaaag?tcgaacccaa?ccgagcgaac?cagccccaga?gggaccggcc??300

tggaccggat?tgccaatgca?agcaggagag?cccattccga?agcaaatgga?atggtggccc??360

tggcaggtac?aaggcctcgc?ggtggagcca?tcggccgcaa?cggaatggct?cgcccgttta??420

cccctatcgg?gccgacatcc?agacctcgga?gatgaattgc?gctggtggag?ccatctccaa??480

cgttggtccc?tcagcttggt?ggcccggggg?cgctggattc?cccagatgga?attaagcaaa??540

ggcgagggtt?acccccaccg?agcgcgctgg?gttcccttgt?tgaaccgtga?ggaagatcga??600

cgacggctcg?aagacctcgc?ggccacgctg?cccctcgtgg?cgacctgtgc?cctcccttgg??660

cgtgagccac?ttggacgccg?tagcaaccgc?accaccaggc?ttcgaccgga?agcgatgcga??720

gccgccaacc?cggtggcttg?ctgccgcccc?cggagcggtc?gcctcagggt?ggccaccttg??780

cttgaagact?tggtggatgc?agagctgcgc?aagggatttg?aacccaccac?agaggggctc??840

gaccccctac?tcaccctgtg?gcaagaggcc?ctggcctcag?aaaccggtgt?tgtggaggtg??900

ggcaacgagg?atgcagaacg?ccttaccgcg?gcaagcctgc?actggcgcga?agggattgcc??960

ggaggcttcg?ctgctgcccg?cacctgcctc?gaactaaaca?ccccaaacga?aggcgaagaa?1020

ctctgggacc?tgaagtttgg?cttgcaagcg?gaggccgatc?ccagcctcaa?gctgccggcc?1080

gccgcggcct?gggcctcagg?agccgaaaca?ctccagctcg?gggagatcaa?agttgaccag?1140

gcgggggaag?tgctgctgga?gggtcttggc?cgagccctca?cggtgttccc?tccgatcgaa????1200

cgcggactgg?aaagcgcaac?gccagaaacg?atgcagctca?cgccagcgga?ggcgtttgtc????1260

ttggtgcgaa?cagcaacgca?ccagctccgc?aatgccggca?tcggcgtcga?actgcccccc????1320

agcctttcag?ggggcctcgc?cagccggctt?ggtttagcca?tcaaggcaga?tttaccagat????1380

cgatccagcg?gcttcaccct?cggagaatct?ctggactgga?gctgggatct?gatgatcggc????1440

ggcgtcacac?tcaccctgcg?agagctcgaa?cggctcagcg?gtaagcgcag?tccgcttgtg????1500

cgccacaagg?gagcctggat?cgaactgcga?cccaacgatc?tccgcaacgc?cgaacgcttc????1560

tgtggagcca?atccagaact?gagcctcgac?gatgccctaa?ggctcacggc?cacagaaggg????1620

gagctaatga?tgcgcttgcc?ggtgcatcgc?tttgatgcgg?ggcctcggct?tcagggagtt????1680

ctcgagcaat?atcaccagca?aaaagccccc?gatccccttc?ccgctccaga?gggattttcc????1740

ggacaactgc?gtccttatca?agaacgtggc?ttgggctggc?tggccttctt?acatcgcttc????1800

gatcaaggcg?cctgcctggc?ggacgacatg?ggcttgggca?agaccatcca?attgttggcc????1860

ttcctgcagc?acctcaaagc?cgagcacgaa?ctcaaacgcc?cggtgctgtt?ggtggcccca????1920

acctcggtgc?tcacgaattg?gcgacgggag?gcggaagcct?tcacccccga?gctgtcggtg????1980

aaagagcact?acggcccacg?ccggccttcc?acgccggccg?ccttgaaaaa?agaactcaaa????2040

gatgtggatc?tggtgctcac?cagttacggc?ctgatgcaac?gcgacagcga?gctgctggac????2100

agcgtcgact?ggcaaggggt?tgtgatcgac?gaagcgcagg?cgatcaaaaa?ccctggggcg????2160

aaacaaagcc?aagcagcccg?agacctggcc?cgagctggaa?agagcagcag?gttccgcatc????2220

gcactcaccg?gcacaccggt?ggaaaaccgc?gtcagcgagc?tgtgggcgct?gatggatttc????2280

ctcaacccaa?aggtgttggg?agaggaagac?ttctttcgtc?agcgctaccg?catgccaatt????2340

gagcgctacg?gcgatatgtc?gtcgttacgc?gatctcaaag?cgcgggtcgg?ccccttcatc????2400

ctgcgccgtc?tcaaaaccga?caagtcgatc?atttccgacc?tgcctgaaaa?ggtggagctc????2460

agtgaatggg?tgggtctcag?caaagaacag?aaatcgctgt?acaacaaaac?cgttgaagac??2520

accctcgacg?ccattgccac?cgcaccgcgg?gggcaacgcc?atggccaggt?gctagccctc??2580

ttgacccggt?taaagcagat?ttgcaatcac?ccggctttag?cccaacgcga?aggggccgtt??2640

gacagcgaat?tccttggccg?ttccgccaag?ctgatgcgac?tcgaagaaat?cctcgaagag??2700

gtgattgaag?ccggcgatcg?cgctttgcta?ttcacccaat?tcgccgaatg?ggggcatctc??2760

ctgcaggcct?ggatgcaaca?acgctggaag?tctgaggttc?ccttcctgca?cggcggaacc??2820

cgcaagagtg?atcggcaagc?gatggtggat?cgattccaag?aggacccccg?gggacctcaa??2880

ctctttcttc?tgtccctcaa?ggccggtggt?gtaggcctca?acctcacccg?ggccagtcat??2940

gtgttccacg?tcgatcgctg?gtggaatcca?gcggtggaaa?accaagccac?cgaccgggcc??3000

tatcgaattg?gtcaaaccaa?ccgggtaatg?gtgcacaaat?tcgtcacccg?tggctcggtg??3060

gaagaaaaaa?tcgaccaaat?gattcgtgaa?aaagctcgaa?tggctgaaga?cgtgatcggc??3120

tccggtgaag?actggctcgg?gagccttggc?ggcgatcagc?tgcgcaatct?tgttgccctc??3180

gaggacacct?aa??????????????????????????????????????????????????????3192

<210>90

<211>1063

<212>PRT

＜213〉synechococcus

<400>90

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Pro?Ala?Ile?Arg?Thr?Ser?Ser

1???????????????5???????????????????10??????????????????15

Ser?Ser?Gly?Gln?Pro?Ala?Leu?Leu?Ile?Trp?Ala?Asp?Thr?Trp?Arg?Val

20??????????????????25??????????????????30

Ala?Ser?Pro?Glu?Gly?Pro?Gly?Leu?Thr?Pro?Ala?Leu?His?Pro?Phe?Thr

35??????????????????40??????????????????45

Leu?Gly?Ser?Asp?Asp?Leu?Lys?Ala?Trp?Leu?Thr?Glu?Arg?Asp?Leu?Met

50??????????????????55??????????????????60

Pro?Gly?Gly?Ser?Ile?Asp?Ala?Thr?Ala?Cys?Leu?Thr?Leu?Pro?Ser?Arg

65??????????????????70??????????????????75??????????????????80

Ser?Val?Lys?Pro?Arg?Lys?Ser?Arg?Thr?Gln?Pro?Ser?Glu?Pro?Ala?Pro

85??????????????????90??????????????????95

Glu?Gly?Pro?Ala?Trp?Thr?Gly?Leu?Pro?Met?Gln?Ala?Gly?Glu?Pro?Ile

100?????????????????105?????????????????110

Pro?Lys?Gln?Met?Glu?Trp?Trp?Pro?Trp?Gln?Val?Gln?Gly?Leu?Ala?Val

115?????????????????120?????????????????125

Glu?Pro?Ser?Ala?Ala?Thr?Glu?Trp?Leu?Ala?Arg?Leu?Pro?Leu?Ser?Gly

130?????????????????135?????????????????140

Arg?His?Pro?Asp?Leu?Gly?Asp?Glu?Leu?Arg?Trp?Trp?Ser?His?Leu?Gln

145?????????????????150?????????????????155?????????????????160

Arg?Trp?Ser?Leu?Ser?Leu?Val?Ala?Arg?Gly?Arg?Trp?Ile?Pro?Gln?Met

165?????????????????170?????????????????175

Glu?Leu?Ser?Lys?Gly?Glu?Gly?Tyr?Pro?His?Arg?Ala?Arg?Trp?Val?Pro

180?????????????????185?????????????????190

Leu?Leu?Asn?Arg?Glu?Glu?Asp?Arg?Arg?Arg?Leu?Glu?Asp?Leu?Ala?Ala

195?????????????????200?????????????????205

Thr?Leu?Pro?Leu?Val?Ala?Thr?Cys?Ala?Leu?Pro?Trp?Arg?Glu?Pro?Leu

210?????????????????215?????????????????220

Gly?Arg?Arg?Ser?Asn?Arg?Thr?Thr?Arg?Leu?Arg?Pro?Glu?Ala?Met?Arg

225?????????????????230?????????????????235?????????????????240

Ala?Ala?Asn?Pro?Val?Ala?Cys?Cys?Arg?Pro?Arg?Ser?Gly?Arg?Leu?Arg

245?????????????????250?????????????????255

Val?Ala?Thr?Leu?Leu?Glu?Asp?Leu?Val?Asp?Ala?Glu?Leu?Arg?Lys?Gly

260?????????????????265?????????????????270

Phe?Glu?Pro?Thr?Thr?Glu?Gly?Leu?Asp?Pro?Leu?Leu?Thr?Leu?Trp?Gln

275?????????????????280?????????????????285

Glu?Ala?Leu?Ala?Ser?Glu?Thr?Gly?Val?Val?Glu?Val?Gly?Asn?Glu?Asp

290?????????????????295?????????????????300

Ala?Glu?Arg?Leu?Thr?Ala?Ala?Ser?Leu?His?Trp?Arg?Glu?Gly?Ile?Ala

305?????????????????310?????????????????315?????????????????320

Gly?Gly?Phe?Ala?Ala?Ala?Arg?Thr?Cys?Leu?Glu?Leu?Asn?Thr?Pro?Asn

325?????????????????330?????????????????335

Glu?Gly?Glu?Glu?Leu?Trp?Asp?Leu?Lys?Phe?Gly?Leu?Gln?Ala?Glu?Ala

340?????????????????345?????????????????350

Asp?Pro?Ser?Leu?Lys?Leu?Pro?Ala?Ala?Ala?Ala?Trp?Ala?Ser?Gly?Ala

355?????????????????360?????????????????365

Glu?Thr?Leu?Gln?Leu?Gly?Glu?Ile?Lys?Val?Asp?Gln?Ala?Gly?Glu?Val

370?????????????????375?????????????????380

Leu?Leu?Glu?Gly?Leu?Gly?Arg?Ala?Leu?Thr?Val?Phe?Pro?Pro?Ile?Glu

385?????????????????390?????????????????395?????????????????400

Arg?Gly?Leu?Glu?Ser?Ala?Thr?Pro?Glu?Thr?Met?Gln?Leu?Thr?Pro?Ala

405?????????????????410?????????????????415

Glu?Ala?Phe?Val?Leu?Val?Arg?Thr?Ala?Thr?His?Gln?Leu?Arg?Asn?Ala

420?????????????????425?????????????????430

Gly?Ile?Gly?Val?Glu?Leu?Pro?Pro?Ser?Leu?Ser?Gly?Gly?Leu?Ala?Ser

435?????????????????440?????????????????445

Arg?Leu?Gly?Leu?Ala?Ile?Lys?Ala?Asp?Leu?Pro?Asp?Arg?Ser?Ser?Gly

450?????????????????455?????????????????460

Phe?Thr?Leu?Gly?Glu?Ser?Leu?Asp?Trp?Ser?Trp?Asp?Leu?Met?Ile?Gly

465?????????????????470?????????????????475?????????????????480

Gly?Val?Thr?Leu?Thr?Leu?Arg?Glu?Leu?Glu?Arg?Leu?Ser?Gly?Lys?Arg

485?????????????????490?????????????????495

Ser?Pro?Leu?Val?Arg?His?Lys?Gly?Ala?Trp?Ile?Glu?Leu?Arg?Pro?Asn

500?????????????????505?????????????????510

Asp?Leu?Arg?Asn?Ala?Glu?Arg?Phe?Cys?Gly?Ala?Asn?Pro?Glu?Leu?Ser

515?????????????????520?????????????????525

Leu?Asp?Asp?Ala?Leu?Arg?Leu?Thr?Ala?Thr?Glu?Gly?Glu?Leu?Met?Met

530?????????????????535?????????????????540

Arg?Leu?Pro?Val?His?Arg?Phe?Asp?Ala?Gly?Pro?Arg?Leu?Gln?Gly?Val

545?????????????????550?????????????????555?????????????????560

Leu?Glu?Gln?Tyr?His?Gln?Gln?Lys?Ala?Pro?Asp?Pro?Leu?Pro?Ala?Pro

565?????????????????570?????????????????575

Glu?Gly?Phe?Ser?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Leu?Gly

580?????????????????585?????????????????590

Trp?Leu?Ala?Phe?Leu?His?Arg?Phe?Asp?Gln?Gly?Ala?Cys?Leu?Ala?Asp

595?????????????????600?????????????????605

Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala?Phe?Leu?Gln?His

610?????????????????615?????????????????620

Leu?Lys?Ala?Glu?His?Glu?Leu?Lys?Arg?Pro?Val?Leu?Leu?Val?Ala?Pro

625?????????????????630?????????????????635?????????????????640

Thr?Ser?Val?Leu?Thr?Asn?Trp?Arg?Arg?Glu?Ala?Glu?Ala?Phe?Thr?Pro

645?????????????????650?????????????????655

Glu?Leu?Ser?Val?Lys?Glu?His?Tyr?Gly?Pro?Arg?Arg?Pro?Ser?Thr?Pro

660?????????????????665?????????????????670

Ala?Ala?Leu?Lys?Lys?Glu?Leu?Lys?Asp?Val?Asp?Leu?Val?Leu?Thr?Ser

675?????????????????680?????????????????685

Tyr?Gly?Leu?Met?Gln?Arg?Asp?Ser?Glu?Leu?Leu?Asp?Ser?Val?Asp?Trp

690?????????????????695?????????????????700

Gln?Gly?Val?Val?Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys?Asn?Pro?Gly?Ala

705?????????????????710?????????????????715?????????????????720

Lys?Gln?Ser?Gln?Ala?Ala?Arg?Asp?Leu?Ala?Arg?Ala?Gly?Lys?Ser?Ser

725?????????????????730?????????????????735

Arg?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Val?Ser

740?????????????????745?????????????????750

Glu?Leu?Trp?Ala?Leu?Met?Asp?Phe?Leu?Asn?Pro?Lys?Val?Leu?Gly?Glu

755?????????????????760?????????????????765

Glu?Asp?Phe?Phe?Arg?Gln?Arg?Tyr?Arg?Met?Pro?Ile?Glu?Arg?Tyr?Gly

770?????????????????775?????????????????780

Asp?Met?Ser?Ser?Leu?Arg?Asp?Leu?Lys?Ala?Arg?Val?Gly?Pro?Phe?Ile

785?????????????????790?????????????????795?????????????????800

Leu?Arg?Arg?Leu?Lys?Thr?Asp?Lys?Ser?Ile?Ile?Ser?Asp?Leu?Pro?Glu

805?????????????????810?????????????????815

Lys?Val?Glu?Leu?Ser?Glu?Trp?Val?Gly?Leu?Ser?Lys?Glu?Gln?Lys?Ser

820?????????????????825?????????????????830

Leu?Tyr?Asn?Lys?Thr?Val?Glu?Asp?Thr?Leu?Asp?Ala?Ile?Ala?Thr?Ala

835?????????????????840?????????????????845

Pro?Arg?Gly?Gln?Arg?His?Gly?Gln?Val?Leu?Ala?Leu?Leu?Thr?Arg?Leu

850?????????????????855?????????????????860

Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Leu?Ala?Gln?Arg?Glu?Gly?Ala?Val

865?????????????????870?????????????????875?????????????????880

Asp?Ser?Glu?Phe?Leu?Gly?Arg?Ser?Ala?Lys?Leu?Met?Arg?Leu?Glu?Glu

885?????????????????890?????????????????895

Ile?Leu?Glu?Glu?Val?Ile?Glu?Ala?Gly?Asp?Arg?Ala?Leu?Leu?Phe?Thr

900?????????????????905?????????????????910

Gln?Phe?Ala?Glu?Trp?Gly?His?Leu?Leu?Gln?Ala?Trp?Met?Gln?Gln?Arg

915?????????????????920?????????????????925

Trp?Lys?Ser?Glu?Val?Pro?Phe?Leu?His?Gly?Gly?Thr?Arg?Lys?Ser?Asp

930?????????????????935?????????????????940

Arg?Gln?Ala?Met?Val?Asp?Arg?Phe?Gln?Glu?Asp?Pro?Arg?Gly?Pro?Gln

945?????????????????950?????????????????955?????????????????960

Leu?Phe?Leu?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr

965?????????????????970?????????????????975

Arg?Ala?Ser?His?Val?Phe?His?Val?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val

980?????????????????985?????????????????990

Glu?Asn?Gln?Ala?Thr?Asp?Arg?Ala??Tyr?Arg?Ile?Gly?Gln??Thr?Asn?Arg

995?????????????????1000?????????????????1005

Val?Met??Val?His?Lys?Phe?Val??Thr?Arg?Gly?Ser?Val??Glu?Glu?Lys

1010?????????????????1015?????????????????1020

Ile?Asp??Gln?Met?Ile?Arg?Glu??Lys?Ala?Arg?Met?Ala??Glu?Asp?Val

1025?????????????????1030?????????????????1035

Ile?Gly??Ser?Gly?Glu?Asp?Trp??Leu?Gly?Ser?Leu?Gly??Gly?Asp?Gln

1040?????????????????1045?????????????????1050

Leu?Arg??Asn?Leu?Val?Ala?Leu??Glu?Asp?Thr

1055?????????????????1060

<210>91

<211>3198

<212>DNA

＜213〉synechococcus

<400>91

atgagcctgc?tgcacgccac?ctggctcccg?gccatccgta?cacccaccag?ttccgggcgt?????60

gccgccctgc?tggtgtgggc?ggacacctgg?cgtgtggcgg?agccggcggg?ccccggcgtg????120

accccggcca?cccatccctt?caccctcagc?gccgatgacc?tgcgcgcctg?gctgagcgaa????180

cgggagctgc?tgcccgacgg?catcatcgat?gccaccgcct?gcctcaccct?gcccagccgc????240

acggtgaaac?cgaagcggaa?gcgtggcgag?accgcccctg?tggatgaggg?ctggacgggt????300

ctgcccctgc?aggcgggaga?accgattccg?aagcagaccg?aatggtggcc?ctggcaggta????360

cagggcctgg?cggtcgaacc?cggtgcagcc?accgcctggc?tggcccgctt?gcccctctcc????420

ggccgccacc?ccgacctcgc?cgatgagctg?cgctggtgga?gccacatgca?gcgctgggcc????480

ctcagcctga?ttgctcgcag?tcgctggatt?ccccaggtgg?agctgagcaa?aggggagggc????540

tacccccacc?gcgcccgttg?ggtgcctctg?ctcaatcgcg?aagacgatcg?ccgccgcctg????600

gaagacatgg?cggcccgcct?gccgctggtg?gccacctgcg?ctctcccctg?gcgcgaaccc????660

accgggaagc?gcagcaaccg?caccacccgg?ctgcggcctg?aggcgatgcg?ggcggccaat????720

ccggtggcct?gttgtcgtcc?ccgcagcggc?cgactgcgcg?tcgccaccct?gctcgaagac????780

ctggtggatg?cccagctgcg?cacgggtttc?acagcccaga?cggacgggct?cgatcccctg????840

cttgccgcct?gggaggaggc?cctcggcagc?gacaccggcg?tgatccacct?gggcgatgaa????900

gacgcagagc?gtctggccac?cgccagccat?cactggcgcg?aaggggtggc?cggcactgtg????960

gcggcggcgc?gggcctgcct?ggaactggag?acccccgacg?acggcgatga?cctctggacc???1020

ctgcggttcg?cactgcaggc?cgaagcggat?cccacgctca?aggtgccggc?cgccctcgcc???1080

tgggcggccg?gtccgaaggg?actccagctc?ggcgaaatcg?ccgtggagca?tccgggcgaa???1140

ctgctgctgg?aaggcatggg?ccgggcgctc?acggtgtttc?caccgatcga?acgcggtctc???1200

gacagcgcca?cgccggaagg?gatgcaactc?acccccgccg?aagccttcgt?gctggtgcgc???1260

accgcagccc?gcgaactccg?cgatgtgggg?gtgggcgtgg?agcttccagc?cagcctctcg???1320

ggtggcctgg?cgagcaggct?cggcctggcg?attcaggcgg?aactaccgga?gaaatcccgc????1380

ggtttcacgc?tgggcgaaac?cctcgactgg?agctgggagc?tgatgatcgg?cggcgtcacc????1440

ctgacgctgc?gggaactgga?gcgcctggcg?ggcaagcgca?gccccctggt?gcggcacaag????1500

ggcacctgga?tcgagctgcg?ccccaacgat?ctcaagaatg?cggagcggtt?tttcgccgcg????1560

aagcccgatc?tcagcctcga?cgatgccctg?cgcctcaccg?ccagcgaagg?cgacacgctg????1620

atgcgcatgc?cggtgcaccg?cctggaagcg?ggcccacggc?tgcaggcggt?gctcgagcag????1680

tatcaccaac?agaaagctcc?cgatcccctg?ccggcgccgg?agggcttctg?cggccagctg????1740

cggccttacc?aggagcgggg?cctcggctgg?ctggcctttc?tgcaccgctt?tgatcaaggc????1800

gcctgcctgg?ccgacgacat?gggtctgggc?aagaccatcc?agctgctcgc?ctttctgcag????1860

cacctgaagg?ccgagcagga?gctgaagagg?ccggtgttgc?tcgtggcgcc?cacctcggtg????1920

ctcaccaact?ggaagcggga?ggccgccgcc?ttcacgccgg?agctcgaggt?gaaggagcac????1980

tacgggccca?ggcgccctgc?cacccctgca?gcactcaaga?agagcctcaa?ggatgtggat????2040

ctggtgctca?ccagctacgg?cctgctccaa?cgcgacagcg?aactgctcga?aagtctcgat????2100

tggcaggggg?tggtgatcga?cgaagcgcag?gcaatcaaga?atccgagcgc?caaacagagc????2160

atggcggccc?gagacctggc?ccgcgcagga?cgcagcagcc?gtttccgcat?tgccctcacc????2220

ggcacgccgg?tggagaaccg?ggtgagcgag?ctctgggcct?tgatggattt?cctcaacccg????2280

cgggtgctcg?gcgaagagga?cttcttccgc?cagcgctacc?gcatgccgat?tgagcgctat????2340

ggcgacatgt?cgtcgctgcg?ggatctgaaa?tcccgcgtgg?gacctttcat?tcttcgccgg????2400

ctcaaaaccg?acaaagcgat?catttccgac?ctgcccgaaa?aggtggaact?gagcgaatgg????2460

gtgggattga?gcagggagca?gaaagcgctc?tatgccaaaa?ccgtcgagga?caccctcgat????2520

gcgattgccc?gggcgccccg?cggacaacgg?catggccagg?tgctggggtt?gctcaccaag????2580

ctgaagcaga?tctgtaacca?tcccgccctg?gccctgaaag?aggaggcggc?cggcgacgag????2640

ttcctgcagc?gctccatgaa?actgcagcgc?ctggaggaaa?tcctcgagga?ggtgatcgac??2700

gccggcgacc?gcgccctgct?cttcacccag?ttcgccgaat?ggggccatct?gctgcagggt??2760

tacctgcaac?ggcgctggcg?cagcgaagtg?ccgttcctga?acggcagcac?cagcaagagc??2820

gaacgccagg?cgatggtcga?tcgcttccag?gaagacccgc?gggggcctca?gctgttcctg??2880

ctgtcactga?aagccggtgg?tgtgggcctc?aacctcaccc?gcgccagcca?tgtgtttcac??2940

atcgatcgct?ggtggaatcc?ggcggtggaa?aaccaggcca?ccgaccgcgc?ctaccggatc??3000

ggccagacga?accgggtgat?ggtgcacaag?ttcatcacca?gtggatcggt?cgaagaaaaa??3060

atcgaccgga?tgatccgcga?gaaatcacgc?ctcgccgaag?acatcatcgg?ctcaggcgaa??3120

gattggctcg?gcgggctcga?catgggccag?ctgaaggaac?tggtgagcct?cgacgacaac??3180

ggatcacttt?cagcatga????????????????????????????????????????????????3198

<210>92

<211>1065

<212>PRT

＜213〉synechococcus

<400>92

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Pro?Ala?Ile?Arg?Thr?Pro?Thr

1???????????????5???????????????????10??????????????????15

Ser?Ser?Gly?Arg?Ala?Ala?Leu?Leu?Val?Trp?Ala?Asp?Thr?Trp?Arg?Val

20??????????????????25??????????????????30

Ala?Glu?Pro?Ala?Gly?Pro?Gly?Val?Thr?Pro?Ala?Thr?His?Pro?Phe?Thr

35??????????????????40??????????????????45

Leu?Ser?Ala?Asp?Asp?Leu?Arg?Ala?Trp?Leu?Ser?Glu?Arg?Glu?Leu?Leu

50??????????????????55??????????????????60

Pro?Asp?Gly?Ile?Ile?Asp?Ala?Thr?Ala?Cys?Leu?Thr?Leu?Pro?Ser?Arg

65??????????????????70??????????????????75??????????????????80

Thr?Val?Lys?Pro?Lys?Arg?Lys?Arg?Gly?Glu?Thr?Ala?Pro?Val?Asp?Glu

85??????????????????90??????????????????95

Gly?Trp?Thr?Gly?Leu?Pro?Leu?Gln?Ala?Gly?Glu?Pro?Ile?Pro?Lys?Gln

100?????????????????105?????????????????110

Thr?Glu?Trp?Trp?Pro?Trp?Gln?Val?Gln?Gly?Leu?Ala?Val?Glu?Pro?Gly

115?????????????????120?????????????????125

Ala?Ala?Thr?Ala?Trp?Leu?Ala?Arg?Leu?Pro?Leu?Ser?Gly?Arg?His?Pro

130?????????????????135?????????????????140

Asp?Leu?Ala?Asp?Glu?Leu?Arg?Trp?Trp?Ser?His?Met?Gln?Arg?Trp?Ala

145?????????????????150?????????????????155?????????????????160

Leu?Ser?Leu?Ile?Ala?Arg?Ser?Arg?Trp?Ile?Pro?Gln?Val?Glu?Leu?Ser

165?????????????????170?????????????????175

Lys?Gly?Glu?Gly?Tyr?Pro?His?Arg?Ala?Arg?Trp?Val?Pro?Leu?Leu?Asn

180?????????????????185?????????????????190

Arg?Glu?Asp?Asp?Arg?Arg?Arg?Leu?Glu?Asp?Met?Ala?Ala?Arg?Leu?Pro

195?????????????????200?????????????????205

Leu?Val?Ala?Thr?Cys?Ala?Leu?Pro?Trp?Arg?Glu?Pro?Thr?Gly?Lys?Arg

210?????????????????215?????????????????220

Ser?Asn?Arg?Thr?Thr?Arg?Leu?Arg?Pro?Glu?Ala?Met?Arg?Ala?Ala?Asn

225?????????????????230?????????????????235?????????????????240

Pro?Val?Ala?Cys?Cys?Arg?Pro?Arg?Ser?Gly?Arg?Leu?Arg?Val?Ala?Thr

245?????????????????250?????????????????255

Leu?Leu?Glu?Asp?Leu?Val?Asp?Ala?Gln?Leu?Arg?Thr?Gly?Phe?Thr?Ala

260?????????????????265?????????????????270

Gln?Thr?Asp?Gly?Leu?Asp?Pro?Lau?Leu?Ala?Ala?Trp?Glu?Glu?Ala?Leu

275?????????????????280?????????????????285

Gly?Ser?Asp?Thr?Gly?Val?Ile?His?Leu?Gly?Asp?Glu?Asp?Ala?Glu?Arg

290?????????????????295?????????????????300

Leu?Ala?Thr?Ala?Ser?His?His?Trp?Arg?Glu?Gly?Val?Ala?Gly?Thr?Val

305?????????????????310?????????????????315?????????????????320

Ala?Ala?Ala?Arg?Ala?Cys?Leu?Glu?Leu?Glu?Thr?Pro?Asp?Asp?Gly?Asp

325?????????????????330?????????????????335

Asp?Leu?Trp?Thr?Leu?Arg?Phe?Ala?Leu?Gln?Ala?Glu?Ala?Asp?Pro?Thr

340?????????????????345?????????????????350

Leu?Lys?Val?Pro?Ala?Ala?Leu?Ala?Trp?Ala?Ala?Gly?Pro?Lys?Gly?Leu

355?????????????????360?????????????????365

Gln?Leu?Gly?Glu?Ile?Ala?Val?Glu?His?Pro?Gly?Glu?Leu?Leu?Leu?Glu

370?????????????????375?????????????????380

Gly?Met?Gly?Arg?Ala?Leu?Thr?Val?Phe?Pro?Pro?Ile?Glu?Arg?Gly?Leu

385?????????????????390?????????????????395?????????????????400

Asp?Ser?Ala?Thr?Pro?Glu?Gly?Met?Gln?Leu?Thr?Pro?Ala?Glu?Ala?Phe

405?????????????????410?????????????????415

Val?Leu?Val?Arg?Thr?Ala?Ala?Arg?Glu?Leu?Arg?Asp?Val?Gly?Val?Gly

420?????????????????425?????????????????430

Val?Glu?Leu?Pro?Ala?Ser?Leu?Ser?Gly?Gly?Leu?Ala?Ser?Arg?Leu?Gly

435?????????????????440?????????????????445

Leu?Ala?Ile?Gln?Ala?Glu?Leu?Pro?Glu?Lys?Ser?Arg?Gly?Phe?Thr?Leu

450?????????????????455?????????????????460

Gly?Glu?Thr?Leu?Asp?Trp?Ser?Trp?Glu?Leu?Met?Ile?Gly?Gly?Val?Thr

465?????????????????470?????????????????475?????????????????480

Leu?Thr?Leu?Arg?Glu?Leu?Glu?Arg?Leu?Ala?Gly?Lys?Arg?Ser?Pro?Leu

485?????????????????490?????????????????495

Val?Arg?His?Lys?Gly?Thr?Trp?Ile?Glu?Leu?Arg?Pro?Asn?Asp?Leu?Lys

500?????????????????505?????????????????510

Asn?Ala?Glu?Arg?Phe?Phe?Ala?Ala?Lys?Pro?Asp?Leu?Ser?Leu?Asp?Asp

515?????????????????520?????????????????525

Ala?Leu?Arg?Leu?Thr?Ala?Ser?Glu?Gly?Asp?Thr?Leu?Met?Arg?Met?Pro

530?????????????????535?????????????????540

Val?His?Arg?Leu?Glu?Ala?Gly?Pro?Arg?Leu?Gln?Ala?Val?Leu?Glu?Gln

545?????????????????550?????????????????555?????????????????560

Tyr?His?Gln?Gln?Lys?Ala?Pro?Asp?Pro?Leu?Pro?Ala?Pro?Glu?Gly?Phe

565?????????????????570?????????????????575

Cys?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Leu?Gly?Trp?Leu?Ala

580?????????????????585?????????????????590

Phe?Leu?His?Arg?Phe?Asp?Gln?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly

595?????????????????600?????????????????605

Leu?Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala?Phe?Leu?Gln?His?Leu?Lys?Ala

610?????????????????615?????????????????620

Glu?Gln?Glu?Leu?Lys?Arg?Pro?Val?Leu?Leu?Val?Ala?Pro?Thr?Ser?Val

625?????????????????630?????????????????635?????????????????640

Leu?Thr?Asn?Trp?Lys?Arg?Glu?Ala?Ala?Ala?Phe?Thr?Pro?Glu?Leu?Glu

645?????????????????650?????????????????655

Val?Lys?Glu?His?Tyr?Gly?Pro?Arg?Arg?Pro?Ala?Thr?Pro?Ala?Ala?Leu

660?????????????????665?????????????????670

Lys?Lys?Ser?Leu?Lys?Asp?Val?Asp?Leu?Val?Leu?Thr?Ser?Tyr?Gly?Leu

675?????????????????680?????????????????685

Leu?Gln?Arg?Asp?Ser?Glu?Leu?Leu?Glu?Ser?Leu?Asp?Trp?Gln?Gly?Val

690?????????????????695?????????????????700

Val?Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys?Asn?Pro?Ser?Ala?Lys?Gln?Ser

705?????????????????710?????????????????715?????????????????720

Met?Ala?Ala?Arg?Asp?Leu?Ala?Arg?Ala?Gly?Arg?Ser?Ser?Arg?Phe?Arg

725?????????????????730?????????????????735

Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Val?Ser?Glu?Leu?Trp

740?????????????????745?????????????????750

Ala?Leu?Met?Asp?Phe?Leu?Asn?Pro?Arg?Val?Leu?Gly?Glu?Glu?Asp?Phe

755?????????????????760?????????????????765

Phe?Arg?Gln?Arg?Tyr?Arg?Met?Pro?Ile?Glu?Arg?Tyr?Gly?Asp?Met?Ser

770?????????????????775?????????????????780

Ser?Leu?Arg?Asp?Leu?Lys?Ser?Arg?Val?Gly?Pro?Phe?Ile?Leu?Arg?Arg

785?????????????????790?????????????????795?????????????????800

Leu?Lys?Thr?Asp?Lys?Ala?Ile?Ile?Ser?Asp?Leu?Pro?Glu?Lys?Val?Glu

805?????????????????810?????????????????815

Leu?Ser?Glu?Trp?Val?Gly?Leu?Ser?Arg?Glu?Gln?Lys?Ala?Leu?Tyr?Ala

820?????????????????825?????????????????830

Lys?Thr?Val?Glu?Asp?Thr?Leu?Asp?Ala?Ile?Ala?Arg?Ala?Pro?Arg?Gly

835?????????????????840?????????????????845

Gln?Arg?His?Gly?Gln?Val?Leu?Gly?Leu?Leu?Thr?Lys?Leu?Lys?Gln?Ile

850?????????????????855?????????????????860

Cys?Asn?His?Pro?Ala?Leu?Ala?Leu?Lys?Glu?Glu?Ala?Ala?Gly?Asp?Glu

865?????????????????870?????????????????875?????????????????880

Phe?Leu?Gln?Arg?Ser?Met?Lys?Leu?Gln?Arg?Leu?Glu?Glu?Ile?Leu?Glu

885?????????????????890?????????????????895

Glu?Val?Ile?Asp?Ala?Gly?Asp?Arg?Ala?Leu?Leu?Phe?Thr?Gln?Phe?Ala

900?????????????????905?????????????????910

Glu?Trp?Gly?His?Leu?Leu?Gln?Gly?Tyr?Leu?Gln?Arg?Arg?Trp?Arg?Ser

915?????????????????920?????????????????925

Glu?Val?Pro?Phe?Leu?Asn?Gly?Ser?Thr?Ser?Lys?Ser?Glu?Arg?Gln?Ala

930?????????????????935?????????????????940

Met?Val?Asp?Arg?Phe?Gln?Glu?Asp?Pro?Arg?Gly?Pro?Gln?Leu?Phe?Leu

945?????????????????950?????????????????955?????????????????960

Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr?Arg?Ala?Ser

965?????????????????970?????????????????975

His?Val?Phe?His?Ile?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln

980?????????????????985?????????????????990

Ala?Thr?Asp?Arg?Ala?Tyr?Arg?Ile??Gly?Gln?Thr?Asn?Arg??Val?Met?Val

995?????????????????1000?????????????????1005

His?Lys??Phe?Ile?Thr?Ser?Gly??Ser?Val?Glu?Glu?Lys??Ile?Asp?Arg

1010?????????????????1015?????????????????1020

Met?Ile??Arg?Glu?Lys?Ser?Arg??Leu?Ala?Glu?Asp?Ile??Ile?Gly?Ser

1025?????????????????1030?????????????????1035

Gly?Glu??Asp?Trp?Leu?Gly?Gly??Leu?Asp?Met?Gly?Gln??Leu?Lys?Glu

1040?????????????????1045?????????????????1050

Leu?Val??Ser?Leu?Asp?Asp?Asn??Gly?Ser?Leu?Ser?Ala

1055?????????????????1060?????????????????1065

<210>93

<211>3213

<212>DNA

＜213〉synechococcus

<400>93

atgagcctgc?tgcacgccac?ctggctaccc?gccatccgca?ctcccagcag?ctccggaagg?????60

gctgctttgc?tggtatgggc?tgacacctgg?cgtgtggccg?accccctcgg?ccccggggcc????120

acacccgccc?ttcatccgtt?caccctgagc?gcggaggatc?tgcgcgcctg?gctcacagag????180

cgcgatttgc?ttccggacgg?aatcatcgat?gcgaccgcat?gcctcaccct?gccgagccgc????240

agtgtgaaac?cacggcggcc?ccgtggctca?gctgccgcca?ccccctcatc?agaagagcag????300

cccccttggt?gcgggctgcc?gctgcaagcc?ggcgaaccga?tcccgaaaac?caccgagtgg????360

tggccatggc?aggtgcaggg?gctggcgatc?gaaccgatgg?ccgccacggc?atggctggcc????420

aagcttccac?tgtcaggcca?tcaccctgat?ctggccgatg?agttgcgctg?gtggagtcac????480

atgcagcgat?gggccctcag?tcttgtggct?agggggcgct?ggctgcccca?ggtggaattg????540

agccgaggtg?aggggtatcc?acaccgggcc?cgctgggtcc?cgcttctcaa?tcgagaggaa????600

gaccggcgcc?gcctggagga?ccttgccgcc?cgtctgcccc?tggttgccac?gtgtgcgttg????660

ccctggagag?agcccacagg?aaagcgcagc?aatcgcatca?ccaggctgcg?cccagaggcc????720

atgcgcgctg?ccaatcccgt?ggcctgctgt?cgtccccgca?gcggtcgatt?gcgggtggcc????780

acattgctgg?aggatctggt?agatgcccag?ctgcgcaagg?gcttccatcc?cgatgacgag????840

gggctcgacc?ccctgctctg?cgcctgggaa?aacgccctga?gttcggagac?cggggtgatc????900

gatctgaatg?atgaagatgc?cgaacgcctt?gccacggcga?gccaccactg?gcgcgaggga????960

gtggctggca?atgtggcggc?tgccagggcc?tgccttgaac?tcgccacacc?gaacgagggg???1020

gaagagctct?gggatctgcg?cttctatctg?caggccgaag?ccgatccaac?gctgaaggta???1080

ccggccggag?cagcctgggc?cgctggaccc?gaaggccttc?aactcgggga?gattcctgtg???1140

gagcatcccg?gtgaggtgct?gctcgaaggc?atggggcgtg?ctctcacggt?gttcgaacca???1200

atcgaacggg?gcctggatag?cgccacgccg?gaagcgatgc?agctcacccc?ggcggaagcc???1260

ttcgtgctgg?tgcgcaccgc?cgcccgtcag?ctccgggacg?tgggcgttgg?tgtggatctc???1320

cctcccagcc?tctcgggagg?cctggccagc?cgcctcggtc?tggcgatcaa?ggccgaacta???1380

cccaaacgct?cgcgggggtt?cacccttggg?gaaaatctcg?actggaactg?ggagctgatg???1440

atcgggggcg?tcaccctgac?gctgcgggag?ctggaacggc?tggccggcaa?gcgcagcccc???1500

ttggtgcgcc?acaagggggc?ctggatcgaa?ctcaggccca?atgatctcaa?aaatgcagaa????1560

cgattctgtg?ccgccaatcc?tgatctgagc?ctggacgatg?cccttcgcct?gacggccagc????1620

gaaggggaca?cgctgatgcg?cctccccgtt?catgcctttg?atgctggccc?tcgccttcaa????1680

ggggtgttgg?agcaatacca?ccagcagaaa?gcaccggatc?cacttcctgc?gcccgagggt????1740

ttctgcggtc?agcttcgccc?ttaccaggaa?cgaggcctgg?gctggctggc?cttcctgcac????1800

cgcttcgatc?agggagcctg?cctcgccgac?gacatgggcc?tgggcaagac?gatccagctg????1860

ctggccttcc?tccagcacct?gaagatggaa?caagaactga?aacggccggt?gctgctggtg????1920

gctcccacct?ccgtgctcac?caactggaaa?cgggaagccg?cggccttcac?ccccgagctc????1980

acagtgcatg?agcactacgg?ccccaaacga?ccctccaccc?cagcagcact?gaaaaaagcc????2040

ctgaaagacg?ttgacctggt?gctcaccagc?tacgggcttc?tgcaaagaga?cagtgaactg????2100

cttgaaagtt?tcgactggca?gggaaccgtg?atcgatgaag?ctcaggcgat?caagaaccct????2160

tcggccaagc?aaagccaggc?agcccgtgat?ctggctcgca?cccgcaaggg?ctccaggttc????2220

cgcattgccc?tcactggcac?accggttgaa?aacagagtga?gcgagctctg?ggccctgatg????2280

gatttcctca?atccgaacgt?gctcggcgaa?gaggaatttt?tccggcagcg?ctaccgcatg????2340

ccgatcgaac?gctatggcga?tatgtcgtcg?cttcgcgatc?tcaagtcgcg?ggtgggacca????2400

ttcattctgc?ggcgcttgaa?aaccgacaag?gcgatcatct?ccgacctccc?cgaaaaagtg????2460

gagctgagtg?aatgggtggg?gctgagcaag?gaacagaagt?ccctttacgc?gaaaaccgtg????2520

gagaacaccc?tcgatgccat?cgcccgagct?ccccgaggca?agcgtcacgg?ccaggtgctg????2580

ggactgctga?cgcgcctcaa?acagatctgc?aatcacccgg?ctctggcctt?aaaggaagag????2640

gtggcaggcg?acgacttcct?gcagcgatcg?gtgaagctgc?agcggctcga?agagattctc????2700

gaagaggtga?ttgcagcggg?ggatcgagcc?ctgctgttca?cccagttcgc?ggaatggggg????2760

catctgctgc?agggctacct?gcaacgccgc?tggcgcagcg?aggtgccgtt?cctgagcggc????2820

agcactagca?aaggagaacg?tcaggccatg?gtggatcgct?tccaggaaga?cccgcgcggc??2880

ccccagctgt?tcctgttgtc?cctcaaagcc?ggcggtgtgg?gattgaacct?gacccgggcc??2940

agccacgtgt?tccacatcga?ccgctggtgg?aatcctgcag?ttgaaaacca?ggccactgac??3000

cgtgcttacc?ggattggcca?gaccaatcgg?gtgatggtgc?ataagttcat?caccagtggc??3060

tcagtggaag?agaagatcga?ccggatgatc?cgggagaagt?ccagactggc?ggaagacatc??3120

gtgggctccg?gcgaggagtg?gctcggtggc?ttcgacatgg?gccaactcaa?ggagctggtg??3180

agcctcgagg?acaacgaaac?acgcaaccca?tga???????????????????????????????3213

<210>94

<211>1070

<212>PRT

＜213〉synechococcus

<400>94

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Pro?Ala?Ile?Arg?Thr?Pro?Ser

1???????????????5???????????????????10??????????????????15

Ser?Ser?Gly?Arg?Ala?Ala?Leu?Leu?Val?Trp?Ala?Asp?Thr?Trp?Arg?Val

20??????????????????25??????????????????30

Ala?Asp?Pro?Leu?Gly?Pro?Gly?Ala?Thr?Pro?Ala?Leu?His?Pro?Phe?Thr

35??????????????????40??????????????????45

Leu?Ser?Ala?Glu?Asp?Leu?Arg?Ala?Trp?Leu?Thr?Glu?Arg?Asp?Leu?Leu

50??????????????????55??????????????????60

Pro?Asp?Gly?Ile?Ile?Asp?Ala?Thr?Ala?Cys?Leu?Thr?Leu?Pro?Ser?Arg

65??????????????????70??????????????????75??????????????????80

Ser?Val?Lys?Pro?Arg?Arg?Pro?Arg?Gly?Ser?Ala?Ala?Ala?Thr?Pro?Ser

85??????????????????90??????????????????95

Ser?Glu?Glu?Gln?Pro?Pro?Trp?Cys?Gly?Leu?Pro?Leu?Gln?Ala?Gly?Glu

100?????????????????105?????????????????110

Pro?Ile?Pro?Lys?Thr?Thr?Glu?Trp?Trp?Pro?Trp?Gln?Val?Gln?Gly?Leu

115?????????????????120?????????????????125

Ala?Ile?Glu?Pro?Met?Ala?Ala?Thr?Ala?Trp?Leu?Ala?Lys?Leu?Pro?Leu

130?????????????????135?????????????????140

Ser?Gly?His?His?Pro?Asp?Leu?Ala?Asp?Glu?Leu?Arg?Trp?Trp?Ser?His

145?????????????????150?????????????????155?????????????????160

Met?Gln?Arg?Trp?Ala?Leu?Ser?Leu?Val?Ala?Arg?Gly?Arg?Trp?Leu?Pro

165?????????????????170?????????????????175

Gln?Val?Glu?Leu?Ser?Arg?Gly?Glu?Gly?Tyr?Pro?His?Arg?Ala?Arg?Trp

180?????????????????185?????????????????190

Val?Pro?Leu?Leu?Asn?Arg?Glu?Glu?Asp?Arg?Arg?Arg?Leu?Glu?Asp?Leu

195?????????????????200?????????????????205

Ala?Ala?Arg?Leu?Pro?Leu?Val?Ala?Thr?Cys?Ala?Leu?Pro?Trp?Arg?Glu

210?????????????????215?????????????????220

Pro?Thr?Gly?Lys?Arg?Ser?Asn?Arg?Ile?Thr?Arg?Leu?Arg?Pro?Glu?Ala

225?????????????????230?????????????????235?????????????????240

Met?Arg?Ala?Ala?Asn?Pro?Val?Ala?Cys?Cys?Arg?Pro?Arg?Ser?Gly?Arg

245?????????????????250?????????????????255

Leu?Arg?Val?Ala?Thr?Leu?Leu?Glu?Asp?Leu?Val?Asp?Ala?Gln?Leu?Arg

260?????????????????265?????????????????270

Lys?Gly?Phe?His?Pro?Asp?Asp?Glu?Gly?Leu?Asp?Pro?Leu?Leu?Cys?Ala

275?????????????????280?????????????????285

Trp?Glu?Asn?Ala?Leu?Ser?Ser?Glu?Thr?Gly?Val?Ile?Asp?Leu?Asn?Asp

290?????????????????295?????????????????300

Glu?Asp?Ala?Glu?Arg?Leu?Ala?Thr?Ala?Ser?His?His?Trp?Arg?Glu?Gly

305?????????????????310?????????????????315?????????????????320

Val?Ala?Gly?Asn?Val?Ala?Ala?Ala?Arg?Ala?Cys?Leu?Glu?Leu?Ala?Thr

325?????????????????330?????????????????335

Pro?Asn?Glu?Gly?Glu?Glu?Leu?Trp?Asp?Leu?Arg?Phe?Tyr?Leu?Gln?Ala

340?????????????????345?????????????????350

Glu?Ala?Asp?Pro?Thr?Leu?Lys?Val?Pro?Ala?Gly?Ala?Ala?Trp?Ala?Ala

355?????????????????360?????????????????365

Gly?Pro?Glu?Gly?Leu?Gln?Leu?Gly?Glu?Ile?Pro?Val?Glu?His?Pro?Gly

370?????????????????375?????????????????380

Glu?Val?Leu?Leu?Glu?Gly?Met?Gly?Arg?Ala?Leu?Thr?Val?Phe?Glu?Pro

385?????????????????390?????????????????395?????????????????400

Ile?Glu?Arg?Gly?Leu?Asp?Ser?Ala?Thr?Pro?Glu?Ala?Met?Gln?Leu?Thr

405?????????????????410?????????????????415

Pro?Ala?Glu?Ala?Phe?Val?Leu?Val?Arg?Thr?Ala?Ala?Arg?Gln?Leu?Arg

420?????????????????425?????????????????430

Asp?Val?Gly?Val?Gly?Val?Asp?Leu?Pro?Pro?Ser?Leu?Ser?Gly?Gly?Leu

435?????????????????440?????????????????445

Ala?Ser?Arg?Leu?Gly?Leu?Ala?Ile?Lys?Ala?Glu?Leu?Pro?Lys?Arg?Ser

450?????????????????455?????????????????460

Arg?Gly?Phe?Thr?Leu?Gly?Glu?Asn?Leu?Asp?Trp?Asn?Trp?Glu?Leu?Met

465?????????????????470?????????????????475?????????????????480

Ile?Gly?Gly?Val?Thr?Leu?Thr?Leu?Arg?Glu?Leu?Glu?Arg?Leu?Ala?Gly

485?????????????????490?????????????????495

Lys?Arg?Ser?Pro?Leu?Val?Arg?His?Lys?Gly?Ala?Trp?Ile?Glu?Leu?Arg

500?????????????????505?????????????????510

Pro?Asn?Asp?Leu?Lys?Asn?Ala?Glu?Arg?Phe?Cys?Ala?Ala?Asn?Pro?Asp

515?????????????????520?????????????????525

Leu?Ser?Leu?Asp?Asp?Ala?Leu?Arg?Leu?Thr?Ala?Ser?Glu?Gly?Asp?Thr

530?????????????????535?????????????????540

Leu?Met?Arg?Leu?Pro?Val?His?Ala?Phe?Asp?Ala?Gly?Pro?Arg?Leu?Gln

545?????????????????550?????????????????555?????????????????560

Gly?Val?Leu?Glu?Gln?Tyr?His?Gln?Gln?Lys?Ala?Pro?Asp?Pro?Leu?Pro

565?????????????????570?????????????????575

Ala?Pro?Glu?Gly?Phe?Cys?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly

580?????????????????585?????????????????590

Leu?Gly?Trp?Leu?Ala?Phe?Leu?His?Arg?Phe?Asp?Gln?Gly?Ala?Cys?Leu

595?????????????????600?????????????????605

Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala?Phe?Leu

610?????????????????615?????????????????620

Gln?His?Leu?Lys?Met?Glu?Gln?Glu?Leu?Lys?Arg?Pro?Val?Leu?Leu?Val

625?????????????????630?????????????????635?????????????????640

Ala?Pro?Thr?Ser?Val?Leu?Thr?Asn?Trp?Lys?Arg?Glu?Ala?Ala?Ala?Phe

645?????????????????650?????????????????655

Thr?Pro?Glu?Leu?Thr?Val?His?Glu?His?Tyr?Gly?Pro?Lys?Arg?Pro?Ser

660?????????????????665?????????????????670

Thr?Pro?Ala?Ala?Leu?Lys?Lys?Ala?Leu?Lys?Asp?Val?Asp?Leu?Val?Leu

675?????????????????680?????????????????685

Thr?Ser?Tyr?Gly?Leu?Leu?Gln?Arg?Asp?Ser?Glu?Leu?Leu?Glu?Ser?Phe

690?????????????????695?????????????????700

Asp?Trp?Gln?Gly?Thr?Val?Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys?Asn?Pro

705?????????????????710?????????????????715?????????????????720

Ser?Ala?Lys?Gln?Ser?Gln?Ala?Ala?Arg?Asp?Leu?Ala?Arg?Thr?Arg?Lys

725?????????????????730?????????????????735

Gly?Ser?Arg?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg

740?????????????????745?????????????????750

Val?Ser?Glu?Leu?Trp?Ala?Leu?Met?Asp?Phe?Leu?Asn?Pro?Asn?Val?Leu

755?????????????????760?????????????????765

Gly?Glu?Glu?Glu?Phe?Phe?Arg?Gln?Arg?Tyr?Arg?Met?Pro?Ile?Glu?Arg

770?????????????????775?????????????????780

Tyr?Gly?Asp?Met?Ser?Ser?Leu?Arg?Asp?Leu?Lys?Ser?Arg?Val?Gly?Pro

785?????????????????790?????????????????795?????????????????800

Phe?Ile?Leu?Arg?Arg?Leu?Lys?Thr?Asp?Lys?Ala?Ile?Ile?Ser?Asp?Leu

805?????????????????810?????????????????815

Pro?Glu?Lys?Val?Glu?Leu?Ser?Glu?Trp?Val?Gly?Leu?Ser?Lys?Glu?Gln

820?????????????????825?????????????????830

Lys?Ser?Leu?Tyr?Ala?Lys?Thr?Val?Glu?Asn?Thr?Leu?Asp?Ala?Ile?Ala

835?????????????????840?????????????????845

Arg?Ala?Pro?Arg?Gly?Lys?Arg?His?Gly?Gln?Val?Leu?Gly?Leu?Leu?Thr

850?????????????????855?????????????????860

Arg?Leu?Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Leu?Ala?Leu?Lys?Glu?Glu

865?????????????????870?????????????????875?????????????????880

Val?Ala?Gly?Asp?Asp?Phe?Leu?Gln?Arg?Ser?Val?Lys?Leu?Gln?Arg?Leu

885?????????????????890?????????????????895

Glu?Glu?Ile?Leu?Glu?Glu?Val?Ile?Ala?Ala?Gly?Asp?Arg?Ala?Leu?Leu

900?????????????????905?????????????????910

Phe?Thr?Gln?Phe?Ala?Glu?Trp?Gly?His?Leu?Leu?Gln?Gly?Tyr?Leu?Gln

915?????????????????920?????????????????925

Arg?Arg?Trp?Arg?Ser?Glu?Val?Pro?Phe?Leu?Ser?Gly?Ser?Thr?Ser?Lys

930?????????????????935?????????????????940

Gly?Glu?Arg?Gln?Ala?Met?Val?Asp?Arg?Phe?Gln?Glu?Asp?Pro?Arg?Gly

945?????????????????950?????????????????955?????????????????960

Pro?Gln?Leu?Phe?Leu?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn

965?????????????????970?????????????????975

Leu?Thr?Arg?Ala?Ser?His?Val?Phe?His?Ile?Asp?Arg?Trp?Trp?Asn?Pro

980?????????????????985?????????????????990

Ala?Val?Glu?Asn?Gln?Ala?Thr?Asp??Arg?Ala?Tyr?Arg?Ile??Gly?Gln?Thr

995?????????????????1000?????????????????1005

Asn?Arg??Val?Met?Val?His?Lys??Phe?Ile?Thr?Ser?Gly??Ser?Val?Glu

1010?????????????????1015?????????????????1020

Glu?Lys??Ile?Asp?Arg?Met?Ile??Arg?Glu?Lys?Ser?Arg??Leu?Ala?Glu

1025?????????????????1030?????????????????1035

Asp?Ile??Val?Gly?Ser?Gly?Glu??Glu?Trp?Leu?Gly?Gly??Phe?Asp?Met

1040?????????????????1045?????????????????1050

Gly?Gln??Leu?Lys?Glu?Leu?Val??Ser?Leu?Glu?Asp?Asn??Glu?Thr?Arg

1055?????????????????1060?????????????????1065

Asn?Pro

1070

<210>95

<211>3192

<212>DNA

＜213〉synechococcus

<400>95

atgagcctgc?tgcacgccac?ctggcttccc?gccatccgta?cctctggcag?ttccggccaa?????60

ccggcactgc?tcatttgggc?tgacacctgg?cgggtggcga?caccagaggg?ccccgggcta????120

actccggcgc?tgcacccgtt?caccctggaa?cccgacgacc?tcaaggcctg?gcttcaggaa????180

cgcgacctgt?tgccaggcgg?cagcatcgat?gccaccgcct?gcctcaccct?gcccagtcgc????240

acggtaaaac?cccgcaagag?ccgcagcaaa?acggccgaac?cagcgcccga?agagcccatc????300

tggaccggtc?tgccgatgca?ggccggagag?ccgattccga?aacagacaga?atggtggccg????360

tggcaagtcc?agggcctcgc?tgtcgagccc?tctgccgcca?cggagtggct?ctcacgcctt????420

cccctgtcag?gacggaatcc?agacctggcc?gatgagctgc?gctggtggag?ccacctgcag????480

cgctgggccc?tcagccttgt?ggcccggggg?cgctggattc?cccagatgga?actgagcaaa????540

ggcgagggat?atccccaccg?ggcccgttgg?gtgcctctgc?tcaaccgcga?ggaggaccgg????600

cgacgtctgg?aggatctggc?cgccagcctg?ccgctggtgg?ccacctgcgc?cctgccctgg????660

cgggaaccga?tgggtcggcg?cagcaaccgc?atgacacggc?tgcgtccgga?ggccatgcgt????720

gccgccaacc?cggtggcctg?ctgccggccc?cgcagtggcc?gcctgcgggt?ggccacgctg????780

ctggaggatc?tggtcgacgc?acagctgcgc?aaggactttg?aaccatccac?cgacggcctc????840

gatcccctgt?tgaccctgtg?gcaagacgcc?ctgggctccg?aaacaggggt?gattgagatc????900

ggtgatgaac?aggccgaacg?gctggccagc?gccagcttcc?attggcgcga?gggcatcgct????960

ggagatttcg?ccgctgcacg?cacctgcctg?gaactgcaga?cacctgcaga?gggagaagag???1020

ctctgggagc?tgcggtttgg?gctgcaggcg?gagtcggatc?cgagcctcaa?gctgcccgcc???1080

gctgcggcct?gggcctccgg?tgccgaccaa?ctccagttgg?gagaagtgac?agtcgagcag???1140

cccggtgaag?tgctgctgga?gggtctggga?cgcgccctca?ccgtgttccc?accgatcgaa???1200

aggggcctgg?agaccgctac?gcctgacacg?atgcagctga?cccccgccga?agccttcgtg???1260

ctggtgcgga?ccgcagcgcg?gcagctgcgg?gatgccggcg?tcggcgtcga?ccttcccccc???1320

agcctgtcgg?ggggcctggc?cagccgcctg?ggtctggcga?tcaaggcgga?gctgccagag???1380

cgctccagcg?gcttcagcct?cggcgaatcc?ctcgactgga?gctgggatct?gatgatcggc???1440

ggggtgacgc?tcaccctgcg?ggaactggag?cggttgagcg?gcaaacgcag?ccccctcgtg???1500

cgccacaagg?gggcctggat?cgaattgcga?ccgaacgatc?tgagaaacgc?cgaacgcttc???1560

tgcggtgcca?acccggagct?cagcctggac?gatgccctgc?ggatcaccgc?caccgaaggc????1620

gatctgctga?tgcgtctgcc?ggtgcatcgc?tttgaggccg?gccccaggct?gcaggcggtg????1680

ctggagcagt?accaccagca?gaaggccccg?gatccgttgc?cagcgccgga?ggggttctgc????1740

ggccagctgc?ggccttacca?ggagcgtggc?ctgggctggc?tggccttcct?caaccgcttc????1800

gaccaaggcg?cctgcctggc?ggacgacatg?ggtctgggta?agaccatcca?gctgctggcc????1860

ttcctgcagc?acctgaaagc?agagcaggaa?ctgaagcgcc?cggtgctgct?ggtggccccc????1920

acatcggtgc?tcacaaactg?gcgacgggaa?gcggaagcct?tcacccccga?actggcggtg????1980

cgcgagcact?acggaccgcg?gcgtccctcc?actccggctg?cgctgaagaa?ggcgttgaag????2040

gatgtcgact?tagtcctcac?cagctacggc?ctactgcaga?gggacagtga?attgctggag????2100

tctcaggatt?ggcagggggt?tgtgatcgat?gaagcccaag?cgatcaagaa?tcccagtgcc????2160

aagcagagcc?aggcagcccg?agacctggcc?agaccagcca?aaggcaaccg?cttccgcatc????2220

gccctcacgg?gcacaccggt?ggagaacagg?gtcagcgagc?tctgggcttt?gatggatttc????2280

ctcagtccca?aggtgctggg?agaagaagac?ttcttccgtc?agcgctaccg?gatgccgatc????2340

gagcgctatg?gcgacatggc?atccctacgg?gacttaaaag?ccagggtcgg?ccccttcatc????2400

ctgcgccggc?tgaaaaccga?caagacgatc?atttccgatc?tgcccgagaa?ggtggaactc????2460

agcgaatggg?tggggttgag?caaggagcag?aaatcgctgt?acagcaaaac?cgttgaagac????2520

accctggatg?ccattgcccg?ggcgcctcgt?ggacagcgcc?atggtcaggt?gctgggactg????2580

ctcacccgcc?tgaagcagat?ctgcaaccat?ccggccctgg?cattgagtga?aaacgctgtt????2640

gacgacggct?ttctggggcg?ctccgccaag?ttgcaacggc?ttgaggaaat?cctcgatgag????2700

gtgatcgaag?caggggatcg?ggcgctgctg?ttcacccagt?tcgccgagtg?gggccatctg????2760

ctgcagtcct?ggatgcaaca?acgttggaag?gcggatgtgc?ccttcctgca?tggagggacg????2820

cgcaaaaacg?aacggcaggc?catggtggat?cgttttcagg?aggacccccg?cggcccgcag????2880

ctgttcctgc?tgtcgctcaa?agccggcggg?gtgggtctga?acctgaccag?ggccagccac??2940

gtgttccaca?tcgatcgctg?gtggaaccct?gcggtagaga?accaggccac?cgaccgtgct??3000

tatcggatcg?gccagaccaa?ccgggtgatg?gtgcacaaat?tcatcacaag?cggatccgta??3060

gaagaaaaaa?ttgaccggat?gatccgagag?aagtcgcgcc?tggcagagga?tgtgatcggt??3120

tccggtgaag?actggctcgg?gtgcctggcc?ggtgatcagc?tgcgcaatct?cgttgccctg??3180

gaggacacct?ga??????????????????????????????????????????????????????3192

<210>96

<211>1063

<212>PRT

＜213〉synechococcus

<400>96

Met?Ser?Leu?Leu?His?Ala?Thr?Trp?Leu?Pro?Ala?Ile?Arg?Thr?Ser?Gly

1???????????????5???????????????????10??????????????????15

Ser?Ser?Gly?Gln?Pro?Ala?Leu?Leu?Ile?Trp?Ala?Asp?Thr?Trp?Arg?Val

20??????????????????25??????????????????30

Ala?Thr?Pro?Glu?Gly?Pro?Gly?Leu?Thr?Pro?Ala?Leu?His?Pro?Phe?Thr

35??????????????????40??????????????????45

Leu?Glu?Pro?Asp?Asp?Leu?Lys?Ala?Trp?Leu?Gln?Glu?Arg?Asp?Leu?Leu

50??????????????????55??????????????????60

Pro?Gly?Gly?Ser?Ile?Asp?Ala?Thr?Ala?Cys?Leu?Thr?Leu?Pro?Ser?Arg

65??????????????????70??????????????????75??????????????????80

Thr?Val?Lys?Pro?Arg?Lys?Ser?Arg?Ser?Lys?Thr?Ala?Glu?Pro?Ala?Pro

85??????????????????90??????????????????95

Glu?Glu?Pro?Ile?Trp?Thr?Gly?Leu?Pro?Met?Gln?Ala?Gly?Glu?Pro?Ile

100?????????????????105?????????????????110

Pro?Lys?Gln?Thr?Glu?Trp?Trp?Pro?Trp?Gln?Val?Gln?Gly?Leu?Ala?Val

115?????????????????120?????????????????125

Glu?Pro?Ser?Ala?Ala?Thr?Glu?Trp?Leu?Ser?Arg?Leu?Pro?Leu?Ser?Gly

130?????????????????135?????????????????140

Arg?Asn?Pro?Asp?Leu?Ala?Asp?Glu?Leu?Arg?Trp?Trp?Ser?His?Leu?Gln

145?????????????????150?????????????????155?????????????????160

Arg?Trp?Ala?Leu?Ser?Leu?Val?Ala?Arg?Gly?Arg?Trp?Ile?Pro?Gln?Met

165?????????????????170?????????????????175

Glu?Leu?Ser?Lys?Gly?Glu?Gly?Tyr?Pro?His?Arg?Ala?Arg?Trp?Val?Pro

180?????????????????185?????????????????190

Leu?Leu?Asn?Arg?Glu?Glu?Asp?Arg?Arg?Arg?Leu?Glu?Asp?Leu?Ala?Ala

195?????????????????200?????????????????205

Ser?Leu?Pro?Leu?Val?Ala?Thr?Cys?Ala?Leu?Pro?Trp?Arg?Glu?Pro?Met

210?????????????????215?????????????????220

Gly?Arg?Arg?Ser?Asn?Arg?Met?Thr?Arg?Leu?Arg?Pro?Glu?Ala?Met?Arg

225?????????????????230?????????????????235?????????????????240

Ala?Ala?Asn?Pro?Val?Ala?Cys?Cys?Arg?Pro?Arg?Ser?Gly?Arg?Leu?Arg

245?????????????????250?????????????????255

Val?Ala?Thr?Leu?Leu?Glu?Asp?Leu?Val?Asp?Ala?Gln?Leu?Arg?Lys?Asp

260?????????????????265?????????????????270

Phe?Glu?Pro?Ser?Thr?Asp?Gly?Leu?Asp?Pro?Leu?Leu?Thr?Leu?Trp?Gln

275?????????????????280?????????????????285

Asp?Ala?Leu?Gly?Ser?Glu?Thr?Gly?Val?Ile?Glu?Ile?Gly?Asp?Glu?Gln

290?????????????????295?????????????????300

Ala?Glu?Arg?Leu?Ala?Ser?Ala?Ser?Phe?His?Trp?Arg?Glu?Gly?Ile?Ala

305?????????????????310?????????????????315?????????????????320

Gly?Asp?Phe?Ala?Ala?Ala?Arg?Thr?Cys?Leu?Glu?Leu?Gln?Thr?Pro?Ala

325?????????????????330?????????????????335

Glu?Gly?Glu?Glu?Leu?Trp?Glu?Leu?Arg?Phe?Gly?Leu?Gln?Ala?Glu?Ser

340?????????????????345?????????????????350

Asp?Pro?Ser?Leu?Lys?Leu?Pro?Ala?Ala?Ala?Ala?Trp?Ala?Ser?Gly?Ala

355?????????????????360?????????????????365

Asp?Gln?Leu?Gln?Leu?Gly?Glu?Val?Thr?Val?Glu?Gln?Pro?Gly?Glu?Val

370?????????????????375?????????????????380

Leu?Leu?Glu?Gly?Leu?Gly?Arg?Ala?Leu?Thr?Val?Phe?Pro?Pro?Ile?Glu

385?????????????????390?????????????????395?????????????????400

Arg?Gly?Leu?Glu?Thr?Ala?Thr?Pro?Asp?Thr?Met?Gln?Leu?Thr?Pro?Ala

405?????????????????410?????????????????415

Glu?Ala?Phe?Val?Leu?Val?Arg?Thr?Ala?Ala?Arg?Gln?Leu?Arg?Asp?Ala

420?????????????????425?????????????????430

Gly?Val?Gly?Val?Asp?Leu?Pro?Pro?Ser?Leu?Ser?Gly?Gly?Leu?Ala?Ser

435?????????????????440?????????????????445

Arg?Leu?Gly?Leu?Ala?Ile?Lys?Ala?Glu?Leu?Pro?Glu?Arg?Ser?Ser?Gly

450?????????????????455?????????????????460

Phe?Ser?Leu?Gly?Glu?Ser?Leu?Asp?Trp?Ser?Trp?Asp?Leu?Met?Ile?Gly

465?????????????????470?????????????????475?????????????????480

Gly?Val?Thr?Leu?Thr?Leu?Arg?Glu?Leu?Glu?Arg?Leu?Ser?Gly?Lys?Arg

485?????????????????490?????????????????495

Ser?Pro?Leu?Val?Arg?His?Lys?Gly?Ala?Trp?Ile?Glu?Leu?Arg?Pro?Asn

500?????????????????505?????????????????510

Asp?Leu?Arg?Asn?Ala?Glu?Arg?Phe?Cys?Gly?Ala?Asn?Pro?Glu?Leu?Ser

515?????????????????520?????????????????525

Leu?Asp?Asp?Ala?Leu?Arg?Ile?Thr?Ala?Thr?Glu?Gly?Asp?Leu?Leu?Met

530?????????????????535?????????????????540

Arg?Leu?Pro?Val?His?Arg?Phe?Glu?Ala?Gly?Pro?Arg?Leu?Gln?Ala?Val

545?????????????????550?????????????????555?????????????????560

Leu?Glu?Gln?Tyr?His?Gln?Gln?Lys?Ala?Pro?Asp?Pro?Leu?Pro?Ala?Pro

565?????????????????570?????????????????575

Glu?Gly?Phe?Cys?Gly?Gln?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Leu?Gly

580?????????????????585?????????????????590

Trp?Leu?Ala?Phe?Leu?Asn?Arg?Phe?Asp?Gln?Gly?Ala?Cys?Leu?Ala?Asp

595?????????????????600?????????????????605

Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala?Phe?Leu?Gln?His

610?????????????????615?????????????????620

Leu?Lys?Ala?Glu?Gln?Glu?Leu?Lys?Arg?Pro?Val?Leu?Leu?Val?Ala?Pro

625?????????????????630?????????????????635?????????????????640

Thr?Ser?Val?Leu?Thr?Asn?Trp?Arg?Arg?Glu?Ala?Glu?Ala?Phe?Thr?Pro

645?????????????????650?????????????????655

Glu?Leu?Ala?Val?Arg?Glu?His?Tyr?Gly?Pro?Arg?Arg?Pro?Ser?Thr?Pro

660?????????????????665?????????????????670

Ala?Ala?Leu?Lys?Lys?Ala?Leu?Lys?Asp?Val?Asp?Leu?Val?Leu?Thr?Ser

675?????????????????680?????????????????685

Tyr?Gly?Leu?Leu?Gln?Arg?Asp?Ser?Glu?Leu?Leu?Glu?Ser?Gln?Asp?Trp

690?????????????????695?????????????????700

Gln?Gly?Val?Val?Ile?Asp?Glu?Ala?Gln?Ala?Ile?Lys?Asn?Pro?Ser?Ala

705?????????????????710?????????????????715?????????????????720

Lys?Gln?Ser?Gln?Ala?Ala?Arg?Asp?Leu?Ala?Arg?ProAla?Lys?Gly?Asn

725?????????????????730?????????????????735

Arg?Phe?Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Val?Ser

740?????????????????745?????????????????750

Glu?Leu?Trp?Ala?Leu?Met?Asp?Phe?Leu?Ser?Pro?Lys?Val?Leu?Gly?Glu

755?????????????????760?????????????????765

Glu?Asp?Phe?Phe?Arg?Gln?Arg?Tyr?Arg?Met?Pro?Ile?Glu?Arg?Tyr?Gly

770?????????????????775?????????????????780

Asp?Met?Ala?Ser?Leu?Arg?Asp?Leu?Lys?Ala?Arg?Val?Gly?Pro?Phe?Ile

785?????????????????790?????????????????795?????????????????800

Leu?Arg?Arg?Leu?Lys?Thr?Asp?Lys?Thr?Ile?Ile?Ser?Asp?Leu?Pro?Glu

805?????????????????810?????????????????815

Lys?Val?Glu?Leu?Ser?Glu?Trp?Val?Gly?Leu?Ser?Lys?Glu?Gln?Lys?Ser

820?????????????????825?????????????????830

Leu?Tyr?Ser?Lys?Thr?Val?Glu?Asp?Thr?Leu?Asp?Ala?Ile?Ala?Arg?Ala

835?????????????????840?????????????????845

Pro?Arg?Gly?Gln?Arg?His?Gly?Gln?Val?Leu?Gly?Leu?Leu?Thr?Arg?Leu

850?????????????????855?????????????????860

Lys?Gln?Ile?Cys?Asn?His?Pro?Ala?Leu?Ala?Leu?Ser?Glu?Asn?Ala?Val

865?????????????????870?????????????????875?????????????????880

Asp?Asp?Gly?Phe?Leu?Gly?Arg?Ser?Ala?Lys?Leu?Gln?Arg?Leu?Glu?Glu

885?????????????????890?????????????????895

Ile?Leu?Asp?Glu?Val?Ile?Glu?Ala?Gly?Asp?Arg?Ala?Leu?Leu?Phe?Thr

900?????????????????905?????????????????910

Gln?Phe?Ala?Glu?Trp?Gly?His?Leu?Leu?Gln?Ser?Trp?Met?Gln?Gln?Arg

915?????????????????920?????????????????925

Trp?Lys?Ala?Asp?Val?Pro?Phe?Leu?His?Gly?Gly?Thr?Arg?Lys?Asn?Glu

930?????????????????935?????????????????940

Arg?Gln?Ala?Met?Val?Asp?Arg?Phe?Gln?Glu?Asp?Pro?Arg?Gly?Pro?Gln

945?????????????????950?????????????????955?????????????????960

Leu?Phe?Leu?Leu?Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr

965?????????????????970?????????????????975

Arg?Ala?Ser?His?Val?Phe?His?Ile?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val

980?????????????????985?????????????????990

Glu?Asn?Gln?Ala?Thr?Asp?Arg?Ala??Tyr?Arg?Ile?Gly?Gln??Thr?Asn?Arg

995?????????????????1000?????????????????1005

Val?Met??Val?His?Lys?Phe?Ile??Thr?Ser?Gly?Ser?Val??Glu?Glu?Lys

1010?????????????????1015?????????????????1020

Ile?Asp??Arg?Met?Ile?Arg?Glu??Lys?Ser?Arg?Leu?Ala??Glu?Asp?Val

1025?????????????????1030?????????????????1035

Ile?Gly??Ser?Gly?Glu?Asp?Trp??Leu?Gly?Cys?Leu?Ala??Gly?Asp?Gln

1040?????????????????1045?????????????????1050

Leu?Arg??Asn?Leu?Val?Ala?Leu??Glu?Asp?Thr

1055?????????????????1060

<210>97

<211>3060

<212>DNA

＜213〉elongated synechococcus

<400>97

atggcagtgc?tgcacggtgg?ctggctcggc?gatcgcttct?gcgtttgggc?cgaggcttgg???60

caggctggtg?agcctcagtc?ggcagcagaa?attgcgattc?atccctacgc?gatcgcggcc??120

actgacttaa?atgattggtg?ccagaagtac?cgtctgggat?ccctgacggg?gacgccaaca??180

gaagtcctgc?tctctattcc?cagtgacctg?aagaaagagg?cggttctacc?gtttctgagt??240

ggtcaggaaa?ttccagatgg?ggcgctgctt?tggtcttggc?agatccccgt?gctgtcgcta??300

gaagccgcga?tcgccggtca?atggctggcg?accttgccgc?tgggttcggc?ggaggatcat??360

ccttggctgg?ggccagatct?acgcttttgg?agccacatct?accgctgggc?acaaagtttg??420

ctggctcggg?ggcgctttta?tccggcgctg?gagtcgagcg?atcgcggttt?aacggcagtt?????480

tggttgccac?tgtttaatca?agcgggcgat?cgccagcgct?tcgatcgcta?tagtcagcag?????540

ctgcccttta?gtcagttttg?ctatcaggca?atcgaaacag?cggcagcttg?tccttggcag?????600

cctcaaccgc?aggatctgtt?gctgcgagtc?ctacagactt?ggttgacagc?acgactacaa?????660

ccggcgatcg?cggcgggaac?tctcgtgtct?gctgatctgc?tggcggcttg?gcagcaatcg?????720

ctagcgaatg?gaaaaccgct?aaagctagaa?gacagtgaag?ccagtcgctt?gcaaacggcg?????780

atcgatcgct?ggttactacc?agtgcagaat?ggcgcagctc?aggcttggcg?gatggttttg?????840

cgccttgtcc?cgcctacgga?gcaagagcag?ccctggcaat?tggagtttgg?cttacaagca?????900

gcgaccgatc?ccgatcgctt?tcggccggcc?tctctcctct?ggcaggatcc?gctgccacct?????960

gggctaccag?atcaatctca?ggaattgctg?ttacgcggct?tgggacaggc?ttgtcggctc????1020

tatccccaat?tgcaaaccag?tctggcgaca?gcctgtccag?aattccatcc?actgaccaca????1080

gcggaggtct?atcagctgct?caagcaggtg?attcctcagt?ggcaagagca?gggcattgaa????1140

gtgcaactgc?cgccgggctt?gcgtggtcaa?gggcgacacc?ggctgggagt?ggaagtcagc????1200

gccacgttgc?cgagcgatcg?cccgagtgtg?gggctggaag?cactactgca?gtttcgttgg????1260

gagctgagtc?tgggcggtca?gcggctgacc?aaagcagaag?tggaacgctt?ggcagccctg????1320

gaaacgccct?tggtggaaat?caacggcgac?tggattgagg?tgcggccgca?ggatattgag????1380

tcggcgcgag?agtttttccg?taagcgcaag?gatcagccaa?atttgacctt?ggcggatgcg????1440

atcgcgatcg?ccagtggtga?gtcgccgaat?gttggtcgcc?tgccggtggt?caattttgaa????1500

gcggcgggct?tactcgaaga?agccttggcc?gtgtttcagg?ggcagcgatc?gcctgcggct????1560

ttgcccgctc?cgcccacctt?tcagggcgag?ctgcgaccct?atcaagagcg?gggggtgggc????1620

tggctcagct?ttttgcagcg?cttcgggatt?ggggcttgcc?tcgccgacga?catgggcttg????1680

ggtaagacga?ttcagctgct?ggccttttta?ctgcatctca?aacacagcaa?cgagctgacg????1740

cggccggtgc?tgctagtctg?tccgacttcg?gtgctgggca?actgggaacg?ggaggtgcag????1800

aaatttgcac?cggagcttcg?ctggaagctg?cactatggcc?ccgatcgcgc?tcagggtaag????1860

gctttggcga?cagcgctcaa?ggactgcgat?ttggtgctga?ccagttactc?cttggtggcg????1920

cgagatcaga?aagcgatcgc?ggcgatcgac?tggcaaggca?ttgtgctgga?tgaagcccag????1980

aacatcaaga?atgaccaggc?gaaacagacg?caggcggtgc?gagcgatcgc?ccaaagtccg????2040

acgcaaaagc?cccgctttcg?gattgccctg?acagggacgc?cggttgagaa?tcgcctcagt????2100

gagttgtggt?cgattgtcga?gtttttgcag?ccgggacatt?taggcaccaa?gccattcttt????2160

caaaagcgct?ttgtcacgcc?gatcgagcgt?tttggcgatg?cggattcgct?gacagcattg????2220

cggcagcgcg?tgcaaccgtt?aatcctacgg?cgactgaaaa?ccgatcgcag?cattattgcc????2280

gacttgcctg?agaagcaaga?aatgacggtc?ttttgtccgt?tggtacagga?gcaggccgat????2340

cgctatcagg?tgctagtcaa?tgaagcgcta?gccaatattg?aagcaagtga?aggcattcag????2400

cggcgcggcc?agattttggc?attgctaacg?cgactgaagc?agctctgtaa?tcatccgtcg????2460

ttgttgctcg?aaaagccgaa?gctcgatccg?aattttggcg?atcgctcagc?caagttgcag????2520

cgcttactag?aaatgttggc?ggagctaacg?gatgcgggcg?atcgcgcttt?ggtgtttacg????2580

cagtttgcgg?gctggggtag?tttgctgcag?caatttttgc?aggaacagct?agggcgagag????2640

gtgctgtttt?tgtcgggcag?taccaagaag?ggcgatcgcc?aacagatggt?tgatcgcttc????2700

caaaatgatc?cgcaggcacc?ggcaattttc?atcctgtcat?tgaaggctgg?cggggtgggg????2760

ctcaacctga?cgaaagccaa?tcatgtcttt?cattacgatc?gctggtggaa?tccggcagtt????2820

gaaaaccaag?cgaccgatcg?cgcgtttcgg?attgggcaac?gacgcaatgt?acaggtgcac????2880

aagtttgtct?gcgctggcac?tctagaagaa?aaaattgatc?agatgatcgc?tagcaagcaa????2940

gcattagcac?agcagattgt?cggtagtggt?gaggattggc?taacggaact?agacaccaat????3000

caactccggc?aactcttgat?cctcgatcgc?tcagcttggg?tagaagagga?agagccttag????3060

<210>98

<211>1019

<212>PRT

＜213〉elongated synechococcus

<400>98

Met?Ala?Val?Leu?His?Gly?Gly?Trp?Leu?Gly?Asp?Arg?Phe?Cys?Val?Trp

1???????????????5???????????????????10??????????????????15

Ala?Glu?Ala?Trp?Gln?Ala?Gly?Glu?Pro?Gln?Ser?Ala?Ala?Glu?Ile?Ala

20??????????????????25??????????????????30

Ile?His?Pro?Tyr?Ala?Ile?Ala?Ala?Thr?Asp?Leu?Asn?Asp?Trp?Cys?Gln

35??????????????????40??????????????????45

Lys?Tyr?Arg?Leu?Gly?Ser?Leu?Thr?Gly?Thr?Pro?Thr?Glu?Val?Leu?Leu

50??????????????????55??????????????????60

Ser?Ile?Pro?Ser?Asp?Leu?Lys?Lys?Glu?Ala?Val?Leu?Pro?Phe?Leu?Ser

65??????????????????70??????????????????75??????????????????80

Gly?Gln?Glu?Ile?Pro?Asp?Gly?Ala?Leu?Leu?Trp?Ser?Trp?Gln?Ile?Pro

85??????????????????90??????????????????95

Val?Leu?Ser?Leu?Glu?Ala?Ala?Ile?Ala?Gly?Gln?Trp?Leu?Ala?Thr?Leu

100?????????????????105?????????????????110

Pro?Leu?Gly?Ser?Ala?Glu?Asp?His?Pro?Trp?Leu?Gly?Pro?Asp?Leu?Arg

115?????????????????120?????????????????125

Phe?Trp?Ser?His?Ile?Tyr?Arg?Trp?Ala?Gln?Ser?Leu?Leu?Ala?Arg?Gly

130?????????????????135?????????????????140

Arg?Phe?Tyr?Pro?Ala?Leu?Glu?Ser?Ser?Asp?Arg?Gly?Leu?Thr?Ala?Val

145?????????????????150?????????????????155?????????????????160

Trp?Leu?Pro?Leu?Phe?Asn?Gln?Ala?Gly?Asp?Arg?Gln?Arg?Phe?Asp?Arg

165?????????????????170?????????????????175

Tyr?Ser?Gln?Gln?Leu?Pro?Phe?Ser?Gln?Phe?Cys?Tyr?Gln?Ala?Ile?Glu

180?????????????????185?????????????????190

Thr?Ala?Ala?Ala?Cys?Pro?Trp?Gln?Pro?Gln?Pro?Gln?Asp?Leu?Leu?Leu

195?????????????????200?????????????????205

Arg?Val?Leu?Gln?Thr?Trp?Leu?Thr?Ala?Arg?Leu?Gln?Pro?Ala?Ile?Ala

210?????????????????215?????????????????220

Ala?Gly?Thr?Leu?Val?Ser?Ala?Asp?Leu?Leu?Ala?Ala?Trp?Gln?Gln?Ser

225?????????????????230?????????????????235?????????????????240

Leu?Ala?Asn?Gly?Lys?Pro?Leu?Lys?Leu?Glu?Asp?Ser?Glu?Ala?Ser?Arg

245?????????????????250?????????????????255

Leu?Gln?Thr?Ala?Ile?Asp?Arg?Trp?Leu?Leu?Pro?Val?Gln?Asn?Gly?Ala

260?????????????????265?????????????????270

Ala?Gln?Ala?Trp?Arg?Met?Val?Leu?Arg?Leu?Val?Pro?Pro?Thr?Glu?Gln

275?????????????????280?????????????????285

Glu?Gln?Pro?Trp?Gln?Leu?Glu?Phe?Gly?Leu?Gln?Ala?Ala?Thr?Asp?Pro

290?????????????????295?????????????????300

Asp?Arg?Phe?Arg?Pro?Ala?Ser?Leu?Leu?Trp?Gln?Asp?Pro?Leu?Pro?Pro

305?????????????????310?????????????????315?????????????????320

Gly?Leu?Pro?Asp?Gln?Ser?Gln?Glu?Leu?Leu?Leu?Arg?Gly?Leu?Gly?Gln

325?????????????????330?????????????????335

Ala?Cys?Arg?Leu?Tyr?Pro?Gln?Leu?Gln?Thr?Ser?Leu?Ala?Thr?Ala?Cys

340?????????????????345?????????????????350

Pro?Glu?Phe?His?Pro?Leu?Thr?Thr?Ala?Glu?Val?Tyr?Gln?Leu?Leu?Lys

355?????????????????360?????????????????365

Gln?Val?Ile?Pro?Gln?Trp?Gln?Glu?Gln?Gly?Ile?Glu?Val?Gln?Leu?Pro

370?????????????????375?????????????????380

Pro?Gly?Leu?Arg?Gly?Gln?Gly?Arg?His?Arg?Leu?Gly?Val?Glu?Val?Ser

385?????????????????390?????????????????395?????????????????400

Ala?Thr?Leu?Pro?Ser?Asp?Arg?Pro?Ser?Val?Gly?Leu?Glu?Ala?Leu?Leu

405?????????????????410?????????????????415

Gln?Phe?Arg?Trp?Glu?Leu?Ser?Leu?Gly?Gly?Gln?Arg?Leu?Thr?Lys?Ala

420?????????????????425?????????????????430

Glu?Val?Glu?Arg?Leu?Ala?Ala?Leu?Glu?Thr?Pro?Leu?Val?Glu?Ile?Asn

435?????????????????440?????????????????445

Gly?Asp?Trp?Ile?Glu?Val?Arg?Pro?Gln?Asp?Ile?Glu?Ser?Ala?Arg?Glu

450?????????????????455?????????????????460

Phe?Phe?Arg?Lys?Arg?Lys?Asp?Gln?Pro?Asn?Leu?Thr?Leu?Ala?Asp?Ala

465?????????????????470?????????????????475?????????????????480

Ile?Ala?Ile?Ala?Ser?Gly?Glu?Ser?Pro?Asn?Val?Gly?Arg?Leu?Pro?Val

485?????????????????490?????????????????495

Val?Asn?Phe?Glu?Ala?Ala?Gly?Leu?Leu?Glu?Glu?Ala?Leu?Ala?Val?Phe

500?????????????????505?????????????????510

Gln?Gly?Gln?Arg?Ser?Pro?Ala?Ala?Leu?Pro?Ala?Pro?Pro?Thr?Phe?Gln

515?????????????????520?????????????????525

Gly?Glu?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Val?Gly?Trp?Leu?Ser?Phe

530?????????????????535?????????????????540

Leu?Gln?Arg?Phe?Gly?Ile?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu

545?????????????????550?????????????????555?????????????????560

Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala?Phe?Leu?Leu?His?Leu?Lys?His?Ser

565?????????????????570?????????????????575

Asn?Glu?Leu?Thr?Arg?Pro?Val?Leu?Leu?Val?Cys?Pro?Thr?Ser?Val?Leu

580?????????????????585?????????????????590

Gly?Asn?Trp?Glu?Arg?Glu?Val?Gln?Lys?Phe?Ala?Pro?Glu?Leu?Arg?Trp

595?????????????????600?????????????????605

Lys?Leu?His?Tyr?Gly?Pro?Asp?Arg?Ala?Gln?Gly?Lys?Ala?Leu?Ala?Thr

610?????????????????615?????????????????620

Ala?Leu?Lys?Asp?Cys?Asp?Leu?Val?Leu?Thr?Ser?Tyr?Ser?Leu?Val?Ala

625?????????????????630?????????????????635?????????????????640

Arg?Asp?Gln?Lys?Ala?Ile?Ala?Ala?Ile?Asp?Trp?Gln?Gly?Ile?Val?Leu

645?????????????????650?????????????????655

Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn?Asp?Gln?Ala?Lys?Gln?Thr?Gln?Ala

660?????????????????665?????????????????670

Val?Arg?Ala?Ile?Ala?Gln?Ser?Pro?Thr?Gln?Lys?Pro?Arg?Phe?Arg?Ile

675?????????????????680?????????????????685

Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu?Ser?Glu?Leu?Trp?Ser

690?????????????????695?????????????????700

Ile?Val?Glu?Phe?Leu?Gln?Pro?Gly?His?Leu?Gly?Thr?Lys?Pro?Phe?Phe

705?????????????????710?????????????????715?????????????????720

Gln?Lys?Arg?Phe?Val?Thr?Pro?Ile?Glu?Arg?Phe?Gly?Asp?Ala?Asp?Ser

725?????????????????730?????????????????735

Leu?Thr?Ala?Leu?Arg?Gln?Arg?Val?Gln?Pro?Leu?Ile?Leu?Arg?Arg?Leu

740?????????????????745?????????????????750

Lys?Thr?Asp?Arg?Ser?Ile?Ile?Ala?Asp?Leu?Pro?Glu?Lys?Gln?Glu?Met

755?????????????????760?????????????????765

Thr?Val?Phe?Cys?Pro?Leu?Val?Gln?Glu?Gln?Ala?Asp?Arg?Tyr?Gln?Val

770?????????????????775?????????????????780

Leu?Val?Asn?Glu?Ala?Leu?Ala?Asn?Ile?Glu?Ala?Ser?Glu?Gly?Ile?Gln

785?????????????????790?????????????????795?????????????????800

Arg?Arg?Gly?Gln?Ile?Leu?Ala?Leu?Leu?Thr?Arg?Leu?Lys?Gln?Leu?Cys

805?????????????????810?????????????????815

Asn?His?Pro?Ser?Leu?Leu?Leu?Glu?Lys?Pro?Lys?Leu?Asp?Pro?Asn?Phe

820?????????????????825?????????????????830

Gly?Asp?Arg?Ser?Ala?Lys?Leu?Gln?Arg?Leu?Leu?Glu?Met?Leu?Ala?Glu

835?????????????????840?????????????????845

Leu?Thr?Asp?Ala?Gly?Asp?Arg?Ala?Leu?Val?Phe?Thr?Gln?Phe?Ala?Gly

850?????????????????855?????????????????860

Trp?Gly?Ser?Leu?Leu?Gln?Gln?Phe?Leu?Gln?Glu?Gln?Leu?Gly?Arg?Glu

865?????????????????870?????????????????875?????????????????880

Val?Leu?Phe?Leu?Ser?Gly?Ser?Thr?Lys?Lys?Gly?Asp?Arg?Gln?Gln?Met

885?????????????????890?????????????????895

Val?Asp?Arg?Phe?Gln?Asn?Asp?Pro?Gln?Ala?Pro?Ala?Ile?Phe?Ile?Leu

900?????????????????905?????????????????910

Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr?Lys?Ala?Asn?His

915?????????????????920?????????????????925

Val?Phe?His?Tyr?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala

930?????????????????935?????????????????940

Thr?Asp?Arg?Ala?Phe?Arg?Ile?Gly?Gln?Arg?Arg?Asn?Val?Gln?Val?His

945?????????????????950?????????????????955?????????????????960

Lys?Phe?Val?Cys?Ala?Gly?Thr?Leu?Glu?Glu?Lys?Ile?Asp?Gln?Met?Ile

965?????????????????970?????????????????975

Ala?Ser?Lys?Gln?Ala?Leu?Ala?Gln?Gln?Ile?Val?Gly?Ser?Gly?Glu?Asp

980?????????????????985?????????????????990

Trp?Leu?Thr?Glu?Leu?Asp?Thr?Asn??Gln?Leu?Arg?Gln?Leu??Leu?Ile?Leu

995?????????????????1000?????????????????1005

Asp?Arg??Ser?Ala?Trp?Val?Glu??Glu?Glu?Glu?Pro

1010?????????????????1015

<210>99

<211>3060

<212>DNA

＜213〉elongated synechococcus

<400>99

atggcagtgc?tgcacggtgg?ctggctcggc?gatcgcttct?gcgtttgggc?cgaggcttgg????60

caggctggtg?agcctcagtc?ggcagcagaa?attgcgattc?atccctacgc?gatcgcggcc???120

actgacttaa?atgattggtg?ccagaagtac?cgtctgggat?ccctgacggg?gacgccaaca???180

gaagtcctgc?tctctattcc?cagtgacctg?aagaaagagg?cggttctacc?gtttctgagt???240

ggtcaggaaa?ttccagatgg?ggcgctgctt?tggtcttggc?agatccccgt?gctgtcacta???300

gaagccgcga?tcgccggtca?atggctggcg?accttgccgc?tgggttcggc?ggaggatcat???360

ccttggctgg?ggccagatct?acgcttttgg?agccacatct?accgctgggc?acaaagtttg???420

ctggctcggg?ggcgctttta?tccggcgctg?gagtcgagcg?atcgcggttt?aacggcagtt???480

tggttgccac?tgtttaatca?agcgggcgat?cgccagcgct?tcgatcgcta?tagtcagcag???540

ctgcccttta?gtcagttttg?ctatcaggca?atcgaaacag?cggcagcttg?tccttggcag???600

cctcaaccgc?aggatctgtt?gctgcgagtc?ctacagactt?ggttgacagc?acgactacaa???660

ccggcgatcg?cggcgggaac?tctcgtgtct?gctgatctgc?tggcggcttg?gcagcaatcg???720

ctagcgaatg?gaaaaccgct?aaagctagaa?gacagtgaag?ccagtcgctt?gcaaacggcg???780

atcgatcgct?ggttactacc?agtgcagaat?ggcgcagctc?aggcttggcg?gatggttttg???840

cgccttgtcc?cgcctacgga?gcaagagcag?ccctggcaat?tggagtttgg?cttacaagca???900

gcgaccgatc?ccgatcgctt?ttggccggcc?tctctcctct?ggcaggatcc?gctgccacct???960

gggctaccag?atcaatctca?ggaattgctg?ttacgcggct?tgggacaggc?ttgtcggctc??1020

tatccccaat?tgcaaaccag?tctggcgaca?gcctgtccag?aattccatcc?actgaccaca??1080

gcggaggtct?atcagctgct?caagcaggtg?attcctcagt?ggcaagagca?gggcattgaa??1140

gtgcaactgc?cgccgggctt?gcgtggtcaa?gggcgacacc?ggctgggagt?ggaagtcagc????1200

gccacgttgc?cgagcgatcg?cccgagtgtg?gggctggaag?cactactgca?gtttcgttgg????1260

gagctgagtc?tgggcggtca?gcggctgacc?aaagcagaag?tggaacgctt?ggcagccctg????1320

gaaacgccct?tggtggaaat?caacggcgac?tggattgagg?tgcggccgca?ggatattgag????1380

tcggcgcgag?agtttttccg?taagcgcaag?gatcagccaa?atttgacctt?ggcggatgcg????1440

atcgcgatcg?ccagtggtga?gtcgccgaat?gttggtcgcc?tgccggtggt?caattttgaa????1500

gcggcgggct?tactcgaaga?agccttggcc?gtgtttcagg?ggcagcgatc?gcctgcggct????1560

ttgcccgctc?cgcccacctt?tcagggcgag?ctgcgaccct?atcaagagcg?gggggtgggc????1620

tggctcagct?ttttgcagcg?cttcgggatt?ggggcttgcc?tcgccgacga?catgggcttg????1680

ggtaagacga?ttcagctgct?ggccttttta?ctgcatctca?aacacagcaa?cgagctgacg????1740

cggccggtgc?tgctagtctg?tccgacttcg?gtgctgggca?actgggaacg?ggaggtgcag????1800

aaatttgcac?cggagcttcg?ctggaagctg?cactatggcc?ccgatcgcgc?tcagggtaag????1860

gctttggcga?cagcgctcaa?ggactgcgat?ttggtgctga?ccagttactc?cttggtggcg????1920

cgagatcaga?aagcgatcgc?ggcgatcgac?tggcaaggca?ttgtgctgga?tgaagcccag????1980

aacatcaaga?atgaccaggc?gaaacagacg?caggcggtgc?gagcgatcgc?ccaaagtccg????2040

acgcaaaagc?cccgctttcg?gattgccctg?acagggacgc?cggttgagaa?tcgcctcagt????2100

gagttgtggt?cgattgtcga?gtttttgcag?ccgggacatt?taggcaccaa?gccattcttt????2160

caaaagcgct?ttgtcacgcc?gatcgagcgt?tttggcgatg?cggattcgct?gacagcattg????2220

cggcagcgcg?tgcaaccgtt?aatcctacgg?cgactgaaaa?ccgatcgcag?cattattgcc????2280

gacttgcctg?agaagcaaga?aatgacggtc?ttttgtccgt?tggtacagga?gcaggccgat????2340

cgctatcagg?tgctagtcaa?tgaagcgcta?gccaatattg?aagcaagtga?aggcattcag????2400

cggcgcggcc?agattttggc?attgctaacg?cgactgaagc?agctctgtaa?tcatccgtcg????2460

ttgttgctcg?aaaagccgaa?gctcgatccg?aattttggcg?atcgctcagc?caagttgcag??2520

cgcttactag?aaatgttggc?ggagctaacg?gatgcgggcg?atcgcgcttt?ggtgtttacg??2580

cagtttgcgg?gctggggtag?tttgctgcag?caatttttgc?aggaacagct?agggcgagag??2640

gtgctgtttt?tgtcgggcag?taccaagaag?ggcgatcgcc?aacagatggt?tgatcgcttc??2700

caaaatgatc?cgcaggcacc?ggcaattttc?atcctgtcat?tgaaggctgg?cggggtgggg??2760

ctcaacctga?cgaaagccaa?tcatgtcttt?cattacgatc?gctggtggaa?tccggcagtt??2820

gaaaaccaag?cgaccgatcg?cgcgtttcgg?attgggcaac?gacgcaatgt?acaggtgcac??2880

aagtttgtct?gcgctggcac?tctagaagaa?aaaattgatc?agatgatcgc?tagcaagcaa??2940

gcattagcac?agcagattgt?cggtagtggt?gaggattggc?taacggaact?agacaccaat??3000

caactccggc?aactcttgat?cctcgatcgc?tcagcttggg?tagaagagga?agagccttag??3060

<210>100

<211>1019

<212>PRT

＜213〉elongated synechococcus

<400>100

Met?Ala?Val?Leu?His?Gly?Gly?Trp?Leu?Gly?Asp?Arg?Phe?Cys?Val?Trp

1???????????????5???????????????????10??????????????????15

Ala?Glu?Ala?Trp?Gln?Ala?Gly?Glu?Pro?Gln?Ser?Ala?Ala?Glu?Ile?Ala

20??????????????????25??????????????????30

Ile?His?Pro?Tyr?Ala?Ile?Ala?Ala?Thr?Asp?Leu?Asn?Asp?Trp?Cys?Gln

35??????????????????40??????????????????45

Lys?Tyr?Arg?Leu?Gly?Ser?Leu?Thr?Gly?Thr?Pro?Thr?Glu?Val?Leu?Leu

50??????????????????55??????????????????60

Ser?Ile?Pro?Ser?Asp?Leu?Lys?Lys?Glu?Ala?Val?Leu?Pro?Phe?Leu?Ser

65??????????????????70??????????????????75??????????????????80

Gly?Gln?Glu?Ile?Pro?Asp?Gly?Ala?Leu?Leu?Trp?Ser?Trp?Gln?Ile?Pro

85??????????????????90??????????????????95

Val?Leu?Ser?Leu?Glu?Ala?Ala?Ile?Ala?Gly?Gln?Trp?Leu?Ala?Thr?Leu

100?????????????????105?????????????????110

Pro?Leu?Gly?Ser?Ala?Glu?Asp?His?Pro?Trp?Leu?Gly?Pro?Asp?Leu?Arg

115?????????????????120?????????????????125

Phe?Trp?Ser?His?Ile?Tyr?Arg?Trp?Ala?Gln?Ser?Leu?Leu?Ala?Arg?Gly

130?????????????????135?????????????????140

Arg?Phe?Tyr?Pro?Ala?Leu?Glu?Ser?Ser?Asp?Arg?Gly?Leu?Thr?Ala?Val

145?????????????????150?????????????????155?????????????????160

Trp?Leu?Pro?Leu?Phe?Asn?Gln?Ala?Gly?Asp?Arg?Gln?Arg?Phe?Asp?Arg

165?????????????????170?????????????????175

Tyr?Ser?Gln?Gln?Leu?Pro?Phe?Ser?Gln?Phe?Cys?Tyr?Gln?Ala?Ile?Glu

180?????????????????185?????????????????190

Thr?Ala?Ala?Ala?Cys?Pro?Trp?Gln?Pro?Gln?Pro?Gln?Asp?Leu?Leu?Leu

195?????????????????200?????????????????205

Arg?Val?Leu?Gln?Thr?Trp?Leu?Thr?Ala?Arg?Leu?Gln?Pro?Ala?Ile?Ala

210?????????????????215?????????????????220

Ala?Gly?Thr?Leu?Val?Ser?Ala?Asp?Leu?Leu?Ala?Ala?Trp?Gln?Gln?Ser

225?????????????????230?????????????????235?????????????????240

Leu?Ala?Asn?Gly?Lys?Pro?Leu?Lys?Leu?Glu?Asp?Ser?Glu?Ala?Ser?Arg

245?????????????????250?????????????????255

Leu?Gln?Thr?Ala?Ile?Asp?Arg?Trp?Leu?Leu?Pro?Val?Gln?Asn?Gly?Ala

260?????????????????265?????????????????270

Ala?Gln?Ala?Trp?Arg?Met?Val?Leu?Arg?Leu?Val?Pro?Pro?Thr?Glu?Gln

275?????????????????280?????????????????285

Glu?Gln?Pro?Trp?Gln?Leu?Glu?Phe?Gly?Leu?Gln?Ala?Ala?Thr?Asp?Pro

290?????????????????295?????????????????300

Asp?Arg?Phe?Trp?Pro?Ala?Ser?Leu?Leu?Trp?Gln?Asp?Pro?Leu?Pro?Pro

305?????????????????310?????????????????315?????????????????320

Gly?Lau?Pro?Asp?Gln?Ser?Gln?Glu?Leu?Leu?Leu?Arg?Gly?Leu?Gly?Gln

325?????????????????330?????????????????335

Ala?Cys?Arg?Leu?Tyr?Pro?Gln?Leu?Gln?Thr?Ser?Leu?Ala?Thr?Ala?Cys

340?????????????????345?????????????????350

Pro?Glu?Phe?His?Pro?Leu?Thr?Thr?Ala?Glu?Val?Tyr?Gln?Leu?Leu?Lys

355?????????????????360?????????????????365

Gln?Val?Ile?Pro?Gln?Trp?Gln?Glu?Gln?Gly?Ile?Glu?Val?Gln?Leu?Pro

370?????????????????375?????????????????380

Pro?Gly?Leu?Arg?Gly?Gln?Gly?Arg?His?Arg?Leu?Gly?Val?Glu?Val?Ser

385?????????????????390?????????????????395?????????????????400

Ala?Thr?Leu?Pro?Ser?Asp?Arg?Pro?Ser?Val?Gly?Leu?Glu?Ala?Leu?Leu

405?????????????????410?????????????????415

Gln?Phe?Arg?Trp?Glu?Leu?Ser?Leu?Gly?Gly?Gln?Arg?Leu?Thr?Lys?Ala

420?????????????????425?????????????????430

Glu?Val?Glu?Arg?Leu?Ala?Ala?Leu?Glu?Thr?Pro?Leu?Val?Glu?Ile?Asn

435?????????????????440?????????????????445

Gly?Asp?Trp?Ile?Glu?Val?Arg?Pro?Gln?Asp?Ile?Glu?Ser?Ala?Arg?Glu

450?????????????????455?????????????????460

Phe?Phe?Arg?Lys?Arg?Lys?Asp?Gln?Pro?Asn?Leu?Thr?Leu?Ala?Asp?Ala

465?????????????????470?????????????????475?????????????????480

Ile?Ala?Ile?Ala?Ser?Gly?Glu?Ser?Pro?Asn?Val?Gly?Arg?Leu?Pro?Val

485?????????????????490?????????????????495

Val?Asn?Phe?Glu?Ala?Ala?Gly?Leu?Leu?Glu?Glu?Ala?Leu?Ala?Val?Phe

500?????????????????505?????????????????510

Gln?Gly?Gln?Arg?Ser?Pro?Ala?Ala?Leu?Pro?Ala?Pro?Pro?Thr?Phe?Gln

515?????????????????520?????????????????525

Gly?Glu?Leu?Arg?Pro?Tyr?Gln?Glu?Arg?Gly?Val?Gly?Trp?Leu?Ser?Phe

530?????????????????535?????????????????540

Leu?Gln?Arg?Phe?Gly?Ile?Gly?Ala?Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu

545?????????????????550?????????????????555?????????????????560

Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala?Phe?Leu?Leu?His?Leu?Lys?His?Ser

565?????????????????570?????????????????575

Asn?Glu?Leu?Thr?Arg?Pro?Val?Leu?Leu?Val?Cys?Pro?Thr?Ser?Val?Leu

580?????????????????585?????????????????590

Gly?Asn?Trp?Glu?Arg?Glu?Val?Gln?Lys?Phe?Ala?Pro?Glu?Leu?Arg?Trp

595?????????????????600?????????????????605

Lys?Leu?His?Tyr?Gly?Pro?Asp?Arg?Ala?Gln?Gly?Lys?Ala?Leu?Ala?Thr

610?????????????????615?????????????????620

Ala?Leu?Lys?Asp?Cys?Asp?Leu?Val?Leu?Thr?Ser?Tyr?Ser?Leu?Val?Ala

625?????????????????630?????????????????635?????????????????640

Arg?Asp?Gln?Lys?Ala?Ile?Ala?Ala?Ile?Asp?Trp?Gln?Gly?Ile?Val?Leu

645?????????????????650?????????????????655

Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn?Asp?Gln?Ala?Lys?Gln?Thr?Gln?Ala

660?????????????????665?????????????????670

Val?Arg?Ala?Ile?Ala?Gln?Ser?Pro?Thr?Gln?Lys?Pro?Arg?Phe?Arg?Ile

675?????????????????680?????????????????685

Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu?Ser?Glu?Leu?Trp?Ser

690?????????????????695?????????????????700

Ile?Val?Glu?Phe?Leu?Gln?Pro?Gly?His?Leu?Gly?Thr?Lys?Pro?Phe?Phe

705?????????????????710?????????????????715?????????????????720

Gln?Lys?Arg?Phe?Val?Thr?Pro?Ile?Glu?Arg?Phe?Gly?Asp?Ala?Asp?Ser

725?????????????????730?????????????????735

Leu?Thr?Ala?Leu?Arg?Gln?Arg?Val?Gln?Pro?Leu?Ile?Leu?Arg?Arg?Leu

740?????????????????745?????????????????750

Lys?Thr?Asp?Arg?Ser?Ile?Ile?Ala?Asp?Leu?Pro?Glu?Lys?Gln?Glu?Met

755?????????????????760?????????????????765

Thr?Val?Phe?Cys?Pro?Leu?Val?Gln?Glu?Gln?Ala?Asp?Arg?Tyr?Gln?Val

770?????????????????775?????????????????780

Leu?Val?Asn?Glu?Ala?Leu?Ala?Asn?Ile?Glu?Ala?Ser?Glu?Gly?Ile?Gln

785?????????????????790?????????????????795?????????????????800

Arg?Arg?Gly?Gln?Ile?Leu?Ala?Leu?Leu?Thr?Arg?Leu?Lys?Gln?Leu?Cys

805?????????????????810?????????????????815

Asn?His?Pro?Ser?Leu?Leu?Leu?Glu?Lys?Pro?Lys?Leu?Asp?Pro?Asn?Phe

820?????????????????825?????????????????830

Gly?Asp?Arg?Ser?Ala?Lys?Leu?Gln?Arg?Leu?Leu?Glu?Met?Leu?Ala?Glu

835?????????????????840?????????????????845

Leu?Thr?Asp?Ala?Gly?Asp?Arg?Ala?Leu?Val?Phe?Thr?Gln?Phe?Ala?Gly

850?????????????????855?????????????????860

Trp?Gly?Ser?Leu?Leu?Gln?Gln?Phe?Leu?Gln?Glu?Gln?Leu?Gly?Arg?Glu

865?????????????????870?????????????????875?????????????????880

Val?Leu?Phe?Leu?Ser?Gly?Ser?Thr?Lys?Lys?Gly?Asp?Arg?Gln?Gln?Met

885?????????????????890?????????????????895

Val?Asp?Arg?Phe?Gln?Asn?Asp?Pro?Gln?Ala?Pro?Ala?Ile?Phe?Ile?Leu

900?????????????????905?????????????????910

Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr?Lys?Ala?Asn?His

915?????????????????920?????????????????925

Val?Phe?His?Tyr?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala

930?????????????????935?????????????????940

Thr?Asp?Arg?Ala?Phe?Arg?Ile?Gly?Gln?Arg?Arg?Asn?Val?Gln?Val?His

945?????????????????950?????????????????955?????????????????960

Lys?Phe?Val?Cys?Ala?Gly?Thr?Leu?Glu?Glu?Lys?Ile?Asp?Gln?Met?Ile

965?????????????????970?????????????????975

Ala?Ser?Lys?Gln?Ala?Leu?Ala?Gln?Gln?Ile?Val?Gly?Ser?Gly?Glu?Asp

980?????????????????985?????????????????990

Trp?Leu?Thr?Glu?Leu?Asp?Thr?Asn??Gln?Leu?Arg?Gln?Leu??Leu?Ile?Leu

995?????????????????1000?????????????????1005

Asp?Arg??Ser?Ala?Trp?Val?Glu??Glu?Glu?Glu?Pro

1010?????????????????1015

<210>101

<211>3000

<212>DNA

<213>Thermosynechococcus?elongatus

<400>101

atggctattt?tccatggcac?atggctccca?gagccggcgc?cacagttttt?catttgggcg?????60

gaagaatggc?gatcgctggc?tcaggcaatc?acgccttggg?ctcccccggc?gattccggtt????120

tatccctacg?ccacccagag?aaaaacacct?cttaggaaga?cagcccgccc?aagtgccacc????180

tacgttgctt?taccggccca?gattcagggg?catcaactgt?taccaccacc?gctggcggaa????240

gtgcaggggg?aactcctatt?tttgtggcag?gtgcccggct?ggtcaattcc?cgcttcagaa????300

gttttagaac?aactgcatca?actgagtctt?cacggccaag?acagtggcag?tattggcgat????360

gatttgcgct?attggctgca?cgtgagtcgc?tggttgctgg?atttaattgt?gcgtggccaa????420

tacctgccaa?caccagaggg?ctggcggatt?ctgctgaccc?acgggggcga?tcgcgatcgc????480

ctgcgccact?tcagccaatt?gatgccggat?ctgtgtcgct?gttatcaagc?cgatggcaca????540

gcgttgcagt?tgccacccca?tgctgcagat?ctcctggcgg?attttctaca?gcacacccta?????600

cagggttatc?tccacactgc?ccttgctgac?ctcgaattgc?ccaaagtagg?cttagccaaa?????660

gaacatggcc?actggctagc?cttcctgaaa?acgggtcaaa?ccccggaact?gccacctccc?????720

ctcattgaac?gcctgcaccg?ctggcaagaa?ccctaccgcg?agcagttgca?tctgcgtccc?????780

caatggcgac?tggctctgca?attggttccc?ccagatactg?ccgatggtga?ctggcacttg?????840

gcctttgggc?tgcaaacgga?aggggaaacg?gacaccatgc?taagggccgc?cgagatttgg?????900

caatgcaccc?aagaggccct?cctctatcaa?gggcaggtgc?tctggcagcc?ccaagaaacc?????960

ctgttgcggg?gactgggctt?ggcctcccgc?atctatcgtc?ccctcgatcg?cagtcttcaa????1020

gaacgctccc?ccgtggctct?gactttgcac?accacggaag?tttatgcctt?cttgcaaagt????1080

gcaattgcgc?cccttgagca?gcagggggtt?gcgatcattt?tgccaccgag?tctgcgccgc????1140

aatagcgccc?aacatcgctt?gggtctgaaa?ataattgcca?cattgccgcc?gccggccact????1200

aacggcttga?cgattgacag?cttgatgcag?tttcagtggc?agttgcagtt?ggggcagcat????1260

cccctctcgg?aggcggattt?tgatcaactg?cgccgccaag?ggacgcccct?ggtttatctc????1320

aatggtgagt?gggtcttgct?gcgcccccaa?gaggtcaagg?ccgctcaaga?gtttctccag????1380

tctcccccaa?agacccaact?ctcccttgca?gagacactgc?gcattgctac?gggggatacg????1440

gtaacggtgg?ccaagttgcc?gattcttggc?ttagacacca?atgatgcact?ccagaccctc????1500

ttggatggcc?tcacgggcaa?acaaagcctt?gatccagtgc?caacaccgca?ggagttttgc????1560

ggtgaactgc?gcccctacca?ggcacggggg?gtggcgtggc?tgagtttctt?ggaacgctgg????1620

cggctggggg?cttgcttggc?ggacgatatg?ggcttgggga?aaaccattca?actgttggcc????1680

tttttgctcc?acctcaagga?aacgggacgg?gcctaccgac?cgacactgtt?gatctgtcct????1740

acctcggtgc?tggggaactg?gctgcgggag?tgccaaaagt?ttgccccaac?cttgcgggcc????1800

tatgtccacc?atgggagcga?tcgccccaag?ggcaaggcat?ttctgaaaaa?ggttgaaact????1860

cacgatctaa?ttttgaccag?ttatgccctc?ctccagcgcg?atcgcaccac?cttgcagcag????1920

gttctgtggc?agcatttggt?actggatgaa?gcccaaaaca?tcaagaatgc?caacacccag????1980

cagtcccaag?cagcgcggga?actttccgcc?cagtttcgca?ttgccctgac?gggaaccccc????2040

ctagaaaacc?gcctcctcga?actttggtcc?attatggact?tcctccatcc?ggggtacttg????2100

ggccatcgca?cctactttca?acaccgctat?gtccgtccca?ttgaacgcta?tggcgacacc????2160

acctccctca?atgctctgcg?cacctatgtc?cagcccttta?ttctgcggcg?cctgaaaacc????2220

gaccgcagta?ttattcaaga?cctgccggaa?aaacaggaga?tgctggtgta?ttgtggcctc????2280

accctagagc?agatgcagct?ttacactgct?gtggtggaag?actcccttgc?tgctatcgaa????2340

aatagtcaag?gcattcagcg?gcggggcaat?atcttggcca?ccctgaccaa?gttgaagcaa????2400

atctgtaacc?atcccgccca?gtatctcaag?caagaagact?atgcccccga?tcgctcaggt????2460

aaattgcaac?ggcttataga?aatgctgcaa?gcgcttcagg?aagtgggcga?tcgcgccctt????2520

gtctttaccc?aatttgccga?gtttggcacc?cacctgaaaa?cctatctgga?aaaggcgctc????2580

cagcaggagg?tgtttttcct?ctcaggacgc?acccccaaag?cccagcggga?actcatggtg????2640

gaacgctttc?aacacgatcc?cgaggccccc?agggtcttta?ttctttccct?caaggcaggg????2700

ggcgtcggtc?tcaatttgac?tcgcgctaac?catgtctttc?actacgatcg?ctggtggaac????2760

ccagcggtag?aaaatcaggc?cagcgatcgc?gtcttccgca?ttggtcaggc?ccgcaatgtc????2820

caaatccata?aatttatctg?cacgggtacc?ctcgaagaaa?agatccacga?gcaaatcgaa????2880

cagaaaaaag?cccttgcgga?aatgattgtg?ggtagtggcg?aacactggct?gactgaactc????2940

aacctcgacc?agttgcggca?actgctcacc?ttagacaaag?agcggctgat?caccctctag????3000

<210>102

<211>999

<212>PRT

<213>Thermosynechococcus?elongatus

<400>102

Met?Ala?Ile?Phe?His?Gly?Thr?Trp?Leu?Pro?Glu?Pro?Ala?Pro?Gln?Phe

1???????????????5???????????????????10??????????????????15

Phe?Ile?Trp?Ala?Glu?Glu?Trp?Arg?Ser?Leu?Ala?Gln?Ala?Ile?Thr?Pro

20??????????????????25??????????????????30

Trp?Ala?Pro?Pro?Ala?Ile?Pro?Val?Tyr?Pro?Tyr?Ala?Thr?Gln?Arg?Lys

35??????????????????40??????????????????45

Thr?Pro?Leu?Arg?Lys?Thr?Ala?Arg?Pro?Ser?Ala?Thr?Tyr?Val?Ala?Leu

50??????????????????55??????????????????60

Pro?Ala?Gln?Ile?Gln?Gly?His?Gln?Leu?Leu?Pro?Pro?Pro?Leu?Ala?Glu

65??????????????????70??????????????????75??????????????????80

Val?Gln?Gly?Glu?Leu?Leu?Phe?Leu?Trp?Gln?Val?Pro?Gly?Trp?Ser?Ile

85??????????????????90??????????????????95

Pro?Ala?Ser?Glu?Val?Leu?Glu?Gln?Leu?His?Gln?Leu?Ser?Leu?His?Gly

100?????????????????105?????????????????110

Gln?Asp?Ser?Gly?Ser?Ile?Gly?Asp?Asp?Leu?Arg?Tyr?Trp?Leu?His?Val

115?????????????????120?????????????????125

Ser?Arg?Trp?Leu?Leu?Asp?Leu?Ile?Val?Arg?Gly?Gln?Tyr?Leu?Pro?Thr

130?????????????????135?????????????????140

Pro?Glu?Gly?Trp?Arg?Ile?Leu?Leu?Thr?His?Gly?Gly?Asp?Arg?Asp?Arg

145?????????????????150?????????????????155?????????????????160

Leu?Arg?His?Phe?Ser?Gln?Leu?Met?Pro?Asp?Leu?Cys?Arg?Cys?Tyr?Gln

165?????????????????170?????????????????175

Ala?Asp?Gly?Thr?Ala?Leu?Gln?Leu?Pro?Pro?His?Ala?Ala?Asp?Leu?Leu

180?????????????????185?????????????????190

Ala?Asp?Phe?Leu?Gln?His?Thr?Leu?Gln?Gly?Tyr?Leu?His?Thr?Ala?Leu

195?????????????????200?????????????????205

Ala?Asp?Leu?Glu?Leu?Pro?Lys?Val?Gly?Leu?Ala?Lys?Glu?His?Gly?His

210?????????????????215?????????????????220

Trp?Leu?Ala?Phe?Leu?Lys?Thr?Gly?Gln?Thr?Pro?Glu?Leu?Pro?Pro?Pro

225?????????????????230?????????????????235?????????????????240

Leu?Ile?Glu?Arg?Leu?His?Arg?Trp?Gln?Glu?Pro?Tyr?Arg?Glu?Gln?Leu

245?????????????????250?????????????????255

His?Leu?Arg?Pro?Gln?Trp?Arg?Leu?Ala?Leu?Gln?Leu?Val?Pro?Pro?Asp

260?????????????????265?????????????????270

Thr?Ala?Asp?Gly?Asp?Trp?His?Leu?Ala?Phe?Gly?Leu?Gln?Thr?Glu?Gly

275?????????????????280?????????????????285

Glu?Thr?Asp?Thr?Met?Leu?Arg?Ala?Ala?Glu?Ile?Trp?Gln?Cys?Thr?Gln

290?????????????????295?????????????????300

Glu?Ala?Leu?Leu?Tyr?Gln?Gly?Gln?Val?Leu?Trp?Gln?Pro?Gln?Glu?Thr

305?????????????????310?????????????????315?????????????????320

Leu?Leu?Arg?Gly?Leu?Gly?Leu?Ala?Ser?Arg?Ile?Tyr?Arg?Pro?Leu?Asp

325?????????????????330?????????????????335

Arg?Ser?Leu?Gln?Glu?Arg?Ser?Pro?Val?Ala?Leu?Thr?Leu?His?Thr?Thr

340?????????????????345?????????????????350

Glu?Val?Tyr?Ala?Phe?Leu?Gln?Ser?Ala?Ile?Ala?Pro?Leu?Glu?Gln?Gln

355?????????????????360?????????????????365

Gly?Val?Ala?Ile?Ile?Leu?Pro?Pro?Ser?Leu?Arg?Arg?Asn?Ser?Ala?Gln

370?????????????????375?????????????????380

His?Arg?Leu?Gly?Leu?Lys?Ile?Ile?Ala?Thr?Leu?Pro?Pro?Pro?Ala?Thr

385?????????????????390?????????????????395?????????????????400

Asn?Gly?Leu?Thr?Ile?Asp?Ser?Leu?Met?Gln?Phe?Gln?Trp?Gln?Leu?Gln

405?????????????????410?????????????????415

Leu?Gly?Gln?His?Pro?Leu?Ser?Glu?Ala?Asp?Phe?Asp?Gln?Leu?Arg?Arg

420?????????????????425?????????????????430

Gln?Gly?Thr?Pro?Leu?Val?Tyr?Leu?Asn?Gly?Glu?Trp?Val?Leu?Leu?Arg

435?????????????????440?????????????????445

Pro?Gln?Glu?Val?Lys?Ala?Ala?Gln?Glu?Phe?Leu?Gln?Ser?Pro?Pro?Lys

450?????????????????455?????????????????460

Thr?Gln?Leu?Ser?Leu?Ala?Glu?Thr?Leu?Arg?Ile?Ala?Thr?Gly?Asp?Thr

465?????????????????470?????????????????475?????????????????480

Val?Thr?Val?Ala?Lys?Leu?Pro?Ile?Leu?Gly?Leu?Asp?Thr?Asn?Asp?Ala

485?????????????????490?????????????????495

Leu?Gln?Thr?Leu?Leu?Asp?Gly?Leu?Thr?Gly?Lys?Gln?Ser?Leu?Asp?Pro

500?????????????????505?????????????????510

Val?Pro?Thr?Pro?Gln?Glu?Phe?Cys?Gly?Glu?Leu?Arg?Pro?Tyr?Gln?Ala

515?????????????????520?????????????????525

Arg?Gly?Val?Ala?Trp?Leu?Ser?Phe?Leu?Glu?Arg?Trp?Arg?Leu?Gly?Ala

530?????????????????535?????????????????540

Cys?Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Ile?Gln?Leu?Leu?Ala

545?????????????????550?????????????????555?????????????????560

Phe?Leu?Leu?His?Leu?Lys?Glu?Thr?Gly?Arg?Ala?Tyr?Arg?Pro?Thr?Leu

565?????????????????570?????????????????575

Leu?Ile?Cys?Pro?Thr?Ser?Val?Leu?Gly?Asn?Trp?Leu?Arg?Glu?Cys?Gln

580?????????????????585?????????????????590

Lys?Phe?Ala?Pro?Thr?Leu?Arg?Ala?Tyr?Val?His?His?Gly?Ser?Asp?Arg

595?????????????????600?????????????????605

Pro?Lys?Gly?Lys?Ala?Phe?Leu?Lys?Lys?Val?Glu?Thr?His?Asp?Leu?Ile

610?????????????????615?????????????????620

Leu?Thr?Ser?Tyr?Ala?Leu?Leu?Gln?Arg?Asp?Arg?Thr?Thr?Leu?Gln?Gln

625?????????????????630?????????????????635?????????????????640

Val?Leu?Trp?Gln?His?Leu?Val?Leu?Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn

645?????????????????650?????????????????655

Ala?Asn?Thr?Gln?Gln?Ser?Gln?Ala?Ala?Arg?Glu?Leu?Ser?Ala?Gln?Phe

660?????????????????665?????????????????670

Arg?Ile?Ala?Leu?Thr?Gly?Thr?Pro?Leu?Glu?Asn?Arg?Leu?Leu?Glu?Leu

675?????????????????680?????????????????685

Trp?Ser?Ile?Met?Asp?Phe?Leu?His?Pro?Gly?Tyr?Leu?Gly?His?Arg?Thr

690?????????????????695?????????????????700

Tyr?Phe?Gln?His?Arg?Tyr?Val?Arg?Pro?Ile?Glu?Arg?Tyr?Gly?Asp?Thr

705?????????????????710?????????????????715?????????????????720

Thr?Ser?Leu?Asn?Ala?Leu?Arg?Thr?Tyr?Val?Gln?Pro?Phe?Ile?Leu?Arg

725?????????????????730?????????????????735

Arg?Leu?Lys?Thr?Asp?Arg?Ser?Ile?Ile?Gln?Asp?Leu?Pro?Glu?Lys?Gln

740?????????????????745?????????????????750

Glu?Met?Leu?Val?Tyr?Cys?Gly?Leu?Thr?Leu?Glu?Gln?Met?Gln?Leu?Tyr

755?????????????????760?????????????????765

Thr?Ala?Val?Val?Glu?Asp?Ser?Leu?Ala?Ala?Ile?Glu?Asn?Ser?Gln?Gly

770?????????????????775?????????????????780

Ile?Gln?Arg?Arg?Gly?Asn?Ile?Leu?Ala?Thr?Leu?Thr?Lys?Leu?Lys?Gln

785?????????????????790?????????????????795?????????????????800

Ile?Cys?Asn?His?Pro?Ala?Gln?Tyr?Leu?Lys?Gln?Glu?Asp?Tyr?Ala?Pro

805?????????????????810?????????????????815

Asp?Arg?Ser?Gly?Lys?Leu?Gln?Arg?Leu?Ile?Glu?Met?Leu?Gln?Ala?Leu

820?????????????????825?????????????????830

Gln?Glu?Val?Gly?Asp?Arg?Ala?Leu?Val?Phe?Thr?Gln?Phe?Ala?Glu?Phe

835?????????????????840?????????????????845

Gly?Thr?His?Leu?Lys?Thr?Tyr?Leu?Glu?Lys?Ala?Leu?Gln?Gln?Glu?Val

850?????????????????855?????????????????860

Phe?Phe?Leu?Ser?Gly?Arg?Thr?Pro?Lys?Ala?Gln?Arg?Glu?Leu?Met?Val

865?????????????????870?????????????????875?????????????????880

Glu?Arg?Phe?Gln?His?Asp?Pro?Glu?Ala?Pro?Arg?Val?Phe?Ile?Leu?Ser

885?????????????????890?????????????????895

Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr?Arg?Ala?Asn?His?Val

900?????????????????905?????????????????910

Phe?His?Tyr?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala?Ser

915?????????????????920?????????????????925

Asp?Arg?Val?Phe?Arg?Ile?Gly?Gln?Ala?Arg?Asn?Val?Gln?Ile?His?Lys

930?????????????????935?????????????????940

Phe?Ile?Cys?Thr?Gly?Thr?Leu?Glu?Glu?Lys?Ile?His?Glu?Gln?Ile?Glu

945?????????????????950?????????????????955?????????????????960

Gln?Lys?Lys?Ala?Leu?Ala?Glu?Met?Ile?Val?Gly?Ser?Gly?Glu?His?Trp

965?????????????????970?????????????????975

Leu?Thr?Glu?Leu?Asn?Leu?Asp?Gln?Leu?Arg?Gln?Leu?Leu?Thr?Leu?Asp

980?????????????????985?????????????????990

Lys?Glu?Arg?Leu?Ile?Thr?Leu

995

<210>103

<211>10

<212>PRT

＜213〉artificial sequence

<220>

＜223〉motif 1

<220>

＜221〉variant

<222>(10)..(10)

＜223 〉/replace=" Ser "

<400>103

Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr

1???????????????5???????????????????10

<210>104

<211>12

<212>PRT

＜213〉artificial sequence

<220>

＜223〉motif 1a

<220>

＜221〉variant

<222>(2)..(2)

＜223 〉/replace=" Val "/replace=" Ile "

<220>

＜221〉variant

<222>(3)..(3)

＜223 〉/replace=" Ile "/replace=" Leu "

<220>

＜221〉variant

<222>(4)..(4)

＜223 〉/replace=" Cys "

<220>

＜221〉variant

<222>(6)..(6)

＜223 〉/replace=" Met "/replace=" Val "

<220>

＜221〉variant

<222>(8)..(8)

＜223 〉/replace=" Ile "/replace=" Leu "

<220>

＜221〉variant

<222>(9)..(9)

＜223 〉/replace=" Ile "/replace=" Leu "

<220>

＜221〉uncertain

<222>(10)..(10)

<400>104

Leu?Leu?Val?Ala?Pro?Thr?Ser?Val?Val?Xaa?Asn?Trp

1???????????????5???????????????????10

<210>105

<211>8

<212>PRT

＜213〉artificial sequence

<220>

＜223〉motif 2

<220>

＜221〉variant

<222>(5)..(5)

＜223 〉/replace=" Ala "/replace=" His "

<220>

＜221〉variant

<222>(6)..(6)

＜223 〉/replace=" Ile "/replace=" Leu "

<400>105

Asp?Glu?Ala?Gln?Asn?Val?Lys?Asn

1???????????????5

<210>106

<211>9

<212>PRT

＜213〉artificial sequence

<220>

＜223〉motif 3

<220>

＜221〉variant

<222>(2)..(2)

＜223 〉/replace=" Met "

<220>

＜221〉uncertain

<222>(7)..(7)

<400>106

Ala?Leu?Thr?Gly?Thr?Pro?Xaa?Glu?Asn

1???????????????5

<210>107

<211>6

<212>PRT

＜213〉artificial sequence

<220>

＜223〉motif 4

<220>

＜221〉variant

<222>(1)..(1)

＜223 〉/replace=" Ile "

<220>

＜221〉uncertain

<222>(2)..(2)

<220>

＜221〉variant

<222>(4)..(4)

＜223 〉/replace=" Ser "

<220>

＜221〉variant

<222>(6)..(6)

＜223 〉/replace=" Tyr "

<400>107

Leu?Xaa?Phe?Thr?Gln?Phe

1???????????????5

<210>108

<211>17

<212>PRT

＜213〉artificial sequence

<220>

＜223〉motif 5

<220>

＜221〉variant

<222>(2)..(2)

＜223 〉/replace=" Val "

<220>

＜221〉variant

<222>(7)..(7)

＜223 〉/replace=" Thr "/replace=" Leu "

<220>

＜221〉variant

<222>(9)..(9)

＜223 〉/replace=" Ile "

<220>

＜221〉variant

<222>(10)..(10)

＜223 〉/replace=" Ile "

<220>

＜221〉uncertain

<222>(13)..(13)

<220>

＜221〉uncertain

<222>(13)..(13)

<400>108

Ser?Leu?Lys?Ala?Gly?Gly?Val?Gly?Leu?Asn?Leu?Thr?Xaa?Ala?Asn?His

1???????????????5???????????????????10??????????????????15

Val

<210>109

<211>9

<212>PRT

＜213〉artificial sequence

<220>

＜223〉motif 5a

<400>109

Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu

1???????????????5

<210>110

<211>11

<212>PRT

＜213〉artificial sequence

<220>

＜223〉motif 6

<220>

＜221〉variant

<222>(3)..(3)

＜223 〉/replace=" Ser "

<220>

＜221〉variant

<222>(6)..(6)

＜223 〉/replace=" Thr "/replace=" Val "

<220>

＜221〉variant

<222>(7)..(7)

＜223 〉/replace=" Tyr "

<220>

＜221〉variant

<222>(9)..(9)

＜223 〉/replace=" Leu "

<400>110

Gln?Ala?Thr?Asp?Arg?Ala?Phe?Arg?Ile?Gly?Gln

1???????????????5???????????????????10

<210>111

<211>460

<212>PRT

＜213〉artificial sequence

<220>

＜223〉the ATP enzymatic structure territory of SEQ ID NO:2

<400>111

Leu?Ala?Asp?Asp?Met?Gly?Leu?Gly?Lys?Thr?Pro?Gln?Leu?Leu?Ala?Phe

1???????????????5???????????????????10??????????????????15

Leu?Leu?His?Leu?Ala?Ala?Glu?Asp?Met?Leu?Val?Lys?Pro?Val?Leu?Ile

20??????????????????25??????????????????30

Val?Cys?Pro?Thr?Ser?Val?Leu?Ser?Asn?Trp?Gly?His?Glu?Ile?Asn?Lys

35??????????????????40??????????????????45

Phe?Ala?Pro?Gln?Leu?Lys?Thr?Leu?Leu?His?His?Gly?Asp?Arg?Arg?Lys

50??????????????????55??????????????????60

Lys?Gly?Gln?Pro?Leu?Val?Lys?Gln?Val?Lys?Asp?Gln?Gln?Ile?Val?Leu

65??????????????????70??????????????????75??????????????????80

Thr?Ser?Tyr?Ala?Leu?Leu?Gln?Arg?Asp?Phe?Ser?Ser?Leu?Lys?Leu?Val

85??????????????????90??????????????????95

Asp?Trp?Gln?Gly?Ile?Val?Leu?Asp?Glu?Ala?Gln?Asn?Ile?Lys?Asn?Pro

100?????????????????105?????????????????110

Gln?Ala?Lys?Gln?Ser?Gln?Ala?Ala?Arg?Gln?Leu?Pro?Ala?Gly?Phe?Arg

115?????????????????120?????????????????125

Ile?Ala?Leu?Thr?Gly?Thr?Pro?Val?Glu?Asn?Arg?Leu?Thr?Glu?Leu?Trp

130?????????????????135?????????????????140

Ser?Ile?Leu?Glu?Phe?Leu?Asn?Pro?Gly?Phe?Leu?Gly?Asn?Gln?Ser?Phe

145?????????????????150?????????????????155?????????????????160

Phe?Gln?Arg?Arg?Phe?Ala?Asn?Pro?Ile?Glu?Lys?Phe?Gly?Asp?Arg?Gln

165?????????????????170?????????????????175

Ser?Leu?Leu?Ile?Leu?Arg?Asn?Leu?Val?Arg?Pro?Phe?Ile?Leu?Arg?Arg

180?????????????????185?????????????????190

Leu?Lys?Thr?Asp?Gln?Thr?Ile?Ile?Gln?Asp?Leu?Pro?Glu?Lys?Gln?Glu

195?????????????????200?????????????????205

Met?Thr?Val?Phe?Cys?Asp?Leu?Ser?Gln?Glu?Gln?Ala?Gly?Leu?Tyr?Gln

210?????????????????215?????????????????220

Gln?Leu?Val?Glu?Glu?Ser?Leu?Gln?Ala?Ile?Ala?Asp?Ser?Glu?Gly?Ile

225?????????????????230?????????????????235?????????????????240

Gln?Arg?His?Gly?Leu?Val?Leu?Thr?Leu?Leu?Thr?Lys?Leu?Lys?Gln?Val

245?????????????????250?????????????????255

Cys?Asn?His?Pro?Asp?Leu?Leu?Leu?Lys?Lys?Pro?Ala?Ile?Thr?His?Gly

260?????????????????265?????????????????270

His?Gln?Ser?Gly?Lys?Leu?Ile?Arg?Leu?Ala?Glu?Met?Leu?Glu?Glu?Ile

275?????????????????280?????????????????285

Ile?Ser?Glu?Gly?Asp?Arg?Val?Leu?Ile?Phe?Thr?Gln?Phe?Ala?Ser?Trp

290?????????????????295?????????????????300

Gly?His?Leu?Leu?Lys?Pro?Tyr?Leu?Glu?Lys?Tyr?Phe?Asn?Gln?Glu?Val

305?????????????????310?????????????????315?????????????????320

Leu?Tyr?Leu?His?Gly?Gly?Thr?Pro?Ala?Glu?Gln?Arg?Gln?Ala?Leu?Val

325?????????????????330?????????????????335

Glu?Arg?Phe?Gln?Gln?Asp?Pro?Asn?Ser?Pro?Tyr?Leu?Phe?Ile?Leu?Ser

340?????????????????345?????????????????350

Leu?Lys?Ala?Gly?Gly?Thr?Gly?Leu?Asn?Leu?Thr?Arg?Ala?Asn?His?Val

355?????????????????360?????????????????365

Phe?His?Val?Asp?Arg?Trp?Trp?Asn?Pro?Ala?Val?Glu?Asn?Gln?Ala?Thr

370?????????????????375?????????????????380

Asp?Arg?Ala?Phe?Arg?Ile?Gly?Gln?Thr?Arg?Asn?Val?Gln?Val?His?Lys

385?????????????????390?????????????????395?????????????????400

Phe?Val?Cys?Thr?Gly?Thr?Leu?Glu?Glu?Lys?Ile?Asn?Ala?Met?Met?Ala

405?????????????????410?????????????????415

Asp?Lys?Gln?Gln?Leu?Ala?Glu?Gln?Thr?Val?Asp?Ala?Gly?Glu?Asn?Trp

420?????????????????425?????????????????430

Leu?Thr?Arg?Leu?Asp?Thr?Asp?Lys?Leu?Arg?Gln?Leu?Leu?Thr?Leu?Ser

435?????????????????440?????????????????445

Ala?Thr?Pro?Val?Asp?Tyr?Gln?Ala?Glu?Ala?Ser?Asp

450?????????????????455?????????????????460

<210>112

<211>1244

<212>DNA

＜213〉rice

<400>112

aaaaccaccg?agggacctga?tctgcaccgg?ttttgatagt?tgagggaccc?gttgtgtctg?????60

gttttccgat?cgagggacga?aaatcggatt?cggtgtaaag?ttaagggacc?tcagatgaac????120

ttattccgga?gcatgattgg?gaagggagga?cataaggccc?atgtcgcatg?tgtttggacg????180

gtccagatct?ccagatcact?cagcaggatc?ggccgcgttc?gcgtagcacc?cgcggtttga????240

ttcggcttcc?cgcaaggcgg?cggccggtgg?ccgtgccgcc?gtagcttccg?ccggaagcga????300

gcacgccgcc?gccgccgacc?cggctctgcg?tttgcaccgc?cttgcacgcg?atacatcggg????360

atagatagct?actactctct?ccgtttcaca?atgtaaatca?ttctactatt?ttccacattc????420

atattgatgt?taatgaatat?agacatatat?atctatttag?attcattaac?atcaatatga????480

atgtaggaaa?tgctagaatg?acttacattg?tgaattgtga?aatggacgaa?gtacctacga????540

tggatggatg?caggatcatg?aaagaattaa?tgcaagatcg?tatctgccgc?atgcaaaatc????600

ttactaattg?cgctgcatat?atgcatgaca?gcctgcatgc?gggcgtgtaa?gcgtgttcat????660

ccattaggaa?gtaaccttgt?cattacttat?accagtacta?catactatat?agtattgatt????720

tcatgagcaa?atctacaaaa?ctggaaagca?ataagaaata?cgggactgga?aaagactcaa????780

cattaatcac?caaatatttc?gccttctcca?gcagaatata?tatctctcca?tcttgatcac???840

tgtacacact?gacagtgtac?gcataaacgc?agcagccagc?ttaactgtcg?tctcaccgtc???900

gcacactggc?cttccatctc?aggctagctt?tctcagccac?ccatcgtaca?tgtcaactcg???960

gcgcgcgcac?aggcacaaat?tacgtacaaa?acgcatgacc?aaatcaaaac?caccggagaa??1020

gaatcgctcc?cgcgcgcggc?ggcgacgcgc?acgtacgaac?gcacgcacgc?acgcccaacc??1080

ccacgacacg?atcgcgcgcg?acgccggcga?caccggccgt?ccacccgcgc?cctcacctcg??1140

ccgactataa?atacgtaggc?atctgcttga?tcttgtcatc?catctcacca?ccaaaaaaaa??1200

aaggaaaaaa?aaacaaaaca?caccaagcca?aataaaagcg?acaa???????????????????1244

<210>113

<211>59

<212>DNA

＜213〉artificial sequence

<220>

＜223〉primer: prm08774

<400>113

ggggacaagt?ttgtacaaaa?aagcaggctt?aaacaatggc?gactatccac?ggtaattgg????59

<210>114

<211>49

<212>DNA

＜213〉artificial sequence

<220>

＜223〉primer: prm08779

<400>114

ggggaccact?ttgtacaaga?aagctgggtt?caatcggacg?cttcggctt???????????????49

Claims (47)

1. strengthen the method for output correlated character in plant with respect to control plant, it comprises the expression of nucleic acids of regulating coding HpaG polypeptide in the plant, and described HpaG polypeptide comprises:

I) have at least 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or higher sequence identity with the HpaG peptide sequence of the preferred sequence that increases progressively and SEQ ID NO:2 representative; With

Ii) amino acid is formed, and wherein glycine content is 13% to 25%, and glutamine content is 13% to 20%, and cysteine content is 0% to 1%, and histidine content is 0% to 1%, and does not wherein have tryptophane.

2. the process of claim 1 wherein that described HpaG polypeptide also comprises one or more following motifs:

(i) (N/E) X (Q/R/P) Q (A/S) GX (N/D) G (SEQ ID NO:3) of (motif 1): G (G/E/D), wherein the X on 4 can be arbitrary amino acid, one of preferred S, N, P, R or Q, and wherein the X on 9 can be arbitrary amino acid, one of preferred Q, E, S or P;

(ii) (motif 2): (P/A/V) (F/L/Y) TQ (M/A) LM (H/N/Q) IV (G/M) (E/D/Q) (SEQ ID NO:4) of S (P/Q/A).

3. claim 1 or 2 method wherein realize the described expression of being regulated by the nucleic acid of introducing and express coding HpaG polypeptide in plant.

4. each method of front claim, the nucleic acid of wherein said coding HpaG polypeptide be by arbitrary nucleic acid listed in the Table A or the representative of its part, perhaps can with the sequence representative of given arbitrary nucleic acid hybridization in the Table A.

5. each method of front claim, what provide in the wherein said nucleic acid sequence encoding Table A is arbitrary proteinic directly to homologue or collateral line homologue.

6. each method of front claim, wherein said enhanced yield correlated character comprises the output that increases with respect to control plant, the preferred biomass that increases and/or the seed production of increase.

7. each method of claim 1 to 6 wherein obtains described enhanced yield correlated character under non-stress conditions.

8. each method of claim 1 to 6 wherein obtains described enhanced yield correlated character under the abiotic stress condition.

9. each method of claim 3 to 8, wherein said nucleic acid effectively is connected to constitutive promoter, and preferred GOS2 promotor is most preferably from the GOS2 promotor of rice.

10. each method of claim 3 to 8, wherein said nucleic acid effectively is connected to the chlorenchyma specificity promoter, and preferred protochlorophyllide reductase enzyme promotor is most preferably from the protochlorophyllide reductase enzyme promotor of rice.

11. each method of front claim, the nucleic acid of wherein said coding HpaG polypeptide is the prokaryotic organism sources, preferably from plant pathogenetic bacteria with III type excretory system (TTSS), further preferably from pseudomonadaceae (Pseudomonaceae), more preferably from xanthomonas (Xanthomonas), most preferably from the carpetweed Xanthomonas campestris.

12. by each the available plant of method or its part of aforementioned claim, comprise seed, wherein said plant or its part comprise the recombinant nucleic acid of coding HpaG polypeptide.

13. construct, it comprises:

(a) coding is as the nucleic acid of the HpaG polypeptide of definition in claim 1 or 2;

(b) can drive one or more control sequences that the nucleotide sequence of (a) is expressed; Randomly

(c) transcription termination sequence.

14. the construct of claim 13, one of wherein said control sequence is selected from:

(i) constitutive promoter, preferred GOS2 promotor is most preferably from the GOS2 promotor of rice; Or

(ii) chlorenchyma specificity promoter, preferred protochlorophyllide reductase enzyme promotor is most preferably from the protochlorophyllide reductase enzyme promotor of rice.

15. the purposes of the construct of claim 13 or 14 is used to prepare the output that has increase with respect to control plant, especially the plant of the seed production of biomass of Zeng Jiaing and/or increase.

16. with claim 13 or 14 each construct plant transformed, plant part or vegetable cells.

Have the output of increase 17. produce with respect to control plant, the method for the transgenic plant of the seed production of biomass of Zeng Jiaing and/or increase especially comprises:

(i) in plant, introduce and express the nucleic acid of coding as the HpaG polypeptide of definition in claim 1 or 2; With

(ii) culturing plants cell under the condition that promotes plant-growth and growth.

18. have the output of increase with respect to control plant, especially the transgenic plant of the seed production of biomass of Zeng Jiaing and/or increase or from the transgenic plant cells of described transgenic plant, its expression by the increase of the nucleic acid of coding as the HpaG polypeptide that defines in claim 1 or 2 obtains.

19. claim 12,16 or 18 transgenic plant, or from its transgenic plant cells, wherein said plant is crop plants or monocotyledons or cereal, as rice, corn, wheat, barley, grain, rye, Chinese sorghum and oat.

20. the part gathered in the crops of the plant of claim 19, the wherein said part of gathering in the crops is preferably seed.

21. from the plant of claim 19 and/or from the product of the part gathered in the crops of the plant of claim 18.

22. the nucleic acid of coding HpaG polypeptide increases output with respect to control plant in plant, especially increase the purposes in the seed production.

23. strengthen the method for output correlated character in plant with respect to control plant, being included in increases coding in the plant SWITCH 2/ SUCROSE NON- FERMENTING 2(SWI2/SNF2) expression of the nucleotide sequence of polypeptide, described SWI2/SNF2 polypeptide comprises ATP enzymatic structure territory, and this structural domain comprises at least 5, preferred 6, more preferably 7, the motif below 8 most preferably from the N-end to the C-end:

(i) the motif I LADDMGLGK (T/S) that represents as SEQ ID NO:103 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif I;

(ii) (V/I/L) (V/I/L) XNW of (A/C) P (T/M/V) S (V/I/L) of the motif IaL (L/V/I) that represents as SEQ ID NO:104 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif Ia;

(iii) (V/I/L) KN of the motif II DEAQ (N/A/H) that represents as SEQ ID NO:105 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif II;

(iv) motif III A (L/M) TGTPXEN that represents as SEQ ID NO:106 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif III;

(v) motif IV (L/I) XF (T/S) Q (F/Y) that represents as SEQ ID NO:107 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif IV;

(vi) (N/T) LTXA (N/S/T) HV of motif VS (L/V) KAGG (V/T/L) G (L/I) that represents as SEQ ID NO:108 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif V;

(the vii) motif Va DRWWNPAVE that represents as SEQ ID NO:109 perhaps has at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif Va; With

(viii) (F/Y) R (I/L) GQ of motif VIQA (T/S) DR (A/T/V) that represents as SEQ ID NO:110, perhaps have at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or the motif of higher sequence identity with the sequence of the preferred sequence that increases progressively and motif VI

Wherein the X among motif Ia, motif III, motif IV and the motif V is arbitrary amino acid.

24. the method for claim 23, wherein said SWI2/SNF2 polypeptide is when being used for the constructing system tree, during genealogical tree as shown in Figure 7, tend to the SSO1653 clade of the SWI2/SNF2 polypeptide of the peptide sequence that comprises SEQ ID NO:30 representative rather than with any other SWI2/SNF2 clade cluster.

25. the method for claim 23 or 24, wherein said SWI2/SNF2 polypeptide comprises ATP enzymatic structure territory, and described structural domain has at least 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or higher sequence identity with the represented ATP structural domain of the SEQID NO:111 that comprises among the preferred sequence that increases progressively and the SEQ ID NO:30.

26. the arbitrary peptide sequence that provides among SWI2/SNF2 polypeptide that each method of claim 23 to 25, wherein said SWI2/SNF2 polypeptide are represented with the preferred sequence that increases progressively and SEQ ID NO:30 or the table E of this paper has at least 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or higher sequence identity.

27. each method of claim 23 to 26, the nucleotide sequence of wherein said coding SWI2/SNF2 polypeptide represented by any or its part of the nucleic acid sequence SEQ ID NOs that provides among the table E, perhaps can represent with the sequence of any hybridization of the nucleic acid sequence SEQ ID NOs that provides among the table E.

28. each method of claim 23 to 27, any of the SEQ ID NOs that provides among the wherein said nucleic acid sequence encoding table E directly to homologue or collateral line homologue.

29. each method of claim 23 to 28 wherein realizes the expression of described increase by the nucleotide sequence of introducing and express coding SWI2/SNF2 polypeptide in plant.

30. each method of claim 23 to 29, the proterties that wherein said output is relevant be following one or more: (i) the colored number that increases of each panicle; Total seed weight that (ii) every strain plant increases; (iii) (full) seed number of Zeng Jiaing; Or the harvest index that (iv) increases.

31. each method of claim 23 to 30, wherein with respect to the control plant of under suitable stress conditions, growing, the proterties that described output is correlated with preferably under the water stress conditions, most preferably strengthens in the growing plants under the drought stress condition under the abiotic stress condition.

32. the method for claim 31, wherein said enhanced yield correlated character be following one or more: (i) the over-ground part area of Zeng Jiaing; (ii) total root biomass of Zeng Jiaing; The (iii) thick root biomass of Zeng Jiaing; The (iv) radicula biomass of Zeng Jiaing; (v) each panicle increases spends number; (the vi) full rate of the seed of Zeng Jiaing; (total seed weight that vii) every strain plant increases; (viii) (full) seed number of Zeng Jiaing; Or the harvest index that (ix) increases.

33. each method of claim 23 to 32, the effective conjunctive tissue specificity promoter of wherein said nucleotide sequence, the promotor of expressing described nucleotide sequence in the tissue can be preferentially expanded in preferred effectively connection in early days, most preferably effectively connects β-expansion protein promoter.

34. each method of claim 23 to 33, the nucleotide sequence of wherein said coding SWI2/SNF2 polypeptide is from microbial genome, further preferably from archeobacteria or bacterium, more preferably from cyanobacteria, as synechocystis, Nostoc, synechococcus genus, proto green algae genus, Anaebena sp., Gloeobacter sp., or Thermosynechococcus sp., more preferably from synechocystis, most preferably from cytoalgae PCC6803.

35. by each the available plant of method, plant part (comprising seed) or vegetable cell of claim 23 to 34, wherein said plant, its part or cell comprise the isolating nucleic acid transgenosis of coding SWI2/SNF2 polypeptide.

36. construct, it comprises:

(a) coding is as the nucleotide sequence of the SWI2/SNF2 polypeptide of each definition of claim 23 to 28;

(b) can drive one or more control sequences that the nucleotide sequence of (a) is expressed; Randomly

(c) transcription termination sequence.

37. the construct of claim 36, one of wherein said control sequence are tissue-specific promoters, expression promoter, most preferably β-expansion protein promoter during preferably expansion is organized in early days.

38. the purposes of the construct of claim 36 or 37 is used to prepare the method that has the plant of enhanced yield correlated character with respect to control plant.

39. construct plant transformed, plant part or vegetable cell with claim 36 or 37.

Have the method for the transgenic plant of enhanced yield correlated character 40. produce with respect to control plant, it comprises:

(i) in plant, introduce and express the nucleotide sequence of the SWI2/SNF2 polypeptide that coding defines in each as claim 23 to 28; With

(ii) culturing plants cell under the condition that promotes plant-growth and growth.

41. have the transgenic plant of enhanced yield correlated character or from the transgenic plant cells of described transgenic plant with respect to control plant, the expression of the increase of the nucleotide sequence of the SWI2/SNF2 polypeptide that it defines in each as claim 23 to 28 by coding obtains.

42. claim 35,39 or 41 transgenic plant or from the transgenic plant cells of described transgenic plant, wherein said plant is crop plants or monocotyledons or cereal, as rice, corn, wheat, barley, grain, rye, triticale, Chinese sorghum and oat.

43. the part gathered in the crops of the plant of claim 42, the wherein said part of gathering in the crops is preferably seed.

44. from the plant of claim 42 and/or from the product of the part gathered in the crops of the plant of claim 43.

45. the nucleotide sequence of the SWI2/SNF2 polypeptide that coding as claim 23 to 28 define in each strengthens the output correlated character in plant, preferably increase following purposes in one or more: (i) the colored number of each panicle increase; Total seed weight that (ii) every strain plant increases; (iii) (full) seed number of Zeng Jiaing; Or the harvest index that (iv) increases.

46. the nucleotide sequence of the SWI2/SNF2 polypeptide that coding as claim 23 to 28 define in each is the purposes in the output correlated character in the enhancing plant, wherein with respect to the control plant of under suitable stress conditions, growing, the proterties that described output is correlated with is under the abiotic stress condition, preferably under the water stress conditions, most preferably under the drought stress condition, strengthen in the growing plants.

47. the purposes of the nucleotide sequence of claim 45, wherein said enhanced yield correlated character are following one or more: (i) the over-ground part area of Zeng Jiaing; (ii) total root biomass of Zeng Jiaing; The (iii) thick root biomass of Zeng Jiaing; The (iv) radicula biomass of Zeng Jiaing; (v) each panicle increases spends number; (the vi) full rate of the seed of Zeng Jiaing; (total seed weight that vii) every strain plant increases; (viii) (full) seed number of Zeng Jiaing; Or the harvest index that (ix) increases.

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