CN101653465A - Extraction method of total saponin of asiatica and application thereof in medical field - Google Patents
Extraction method of total saponin of asiatica and application thereof in medical field Download PDFInfo
- Publication number
- CN101653465A CN101653465A CN200910152717A CN200910152717A CN101653465A CN 101653465 A CN101653465 A CN 101653465A CN 200910152717 A CN200910152717 A CN 200910152717A CN 200910152717 A CN200910152717 A CN 200910152717A CN 101653465 A CN101653465 A CN 101653465A
- Authority
- CN
- China
- Prior art keywords
- extraction
- asiatica
- herba centellae
- extracting
- total saponins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Steroid Compounds (AREA)
Abstract
The invention discloses a process for extracting purified asiatica total saponin from a dried whole plant of Centella asiatica (L.) Urb. The process comprises the following flows: weighing a whole plant of asiatica, adding 40-80 percent of alcohol into an ultrasonic extractor for extracting and concentrating an obtained extraction solution to obtain a concentrated solution; extracting the asiaticaby chloroform by utilizing cavatition suspension extracting equipment, and then taking an aqueous part and extracting by using ethylacetate; taking the aqueous part and extracting by n-butyl alcohol;and decompressing and refluxing an obtained n-butyl alcohol part and drying to obtain the asiatica total saponin. Proved by the research of experiments of the asiatica total saponin, the asiatica total saponin has inhibiting effect.
Description
Technical field
The invention belongs to medical technical field, be specifically related to a kind of from Herba Centellae extraction separation obtain asiatic centella total saponins and the effect in mental sickness such as treatment depression etc. thereof.
Background technology
Herba Centellae is dry herb or the whole herb with root of Umbelliferae (Umbelliferae) plant Herba Centellae Centella asiatica (L.) Urban, is a kind of traditional herbal medicines.It has heat-clearing and toxic substances removing, inducing diuresis and reducing edema effect in the theory of Chinese medical science, is used for the treatment of diseases such as jaundice due to damp-heat, heatstroke diarrhoea, sand Stranguria stranguria with blood, carbuncle sore tumefacting virus, injury from falling down; TAO Hong-Jing day: this grass is gained the name with cold and cool, and so its property Great Cold is the name Herba Centellae.
Summary of the invention
Be documented in the earliest in the Shennong's Herbal, all herbal medicine is classified middle product as, is distributed widely in various places on the south the Yangtze river basin.Medical Herba Centellae external and existing more than the 2000 year history of curing the disease for oral administration in China.It has the function of clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling.Be applicable to jaundice due to damp-heat, heatstroke diarrhoea, sand Stranguria stranguria with blood, carbuncle sore tumefacting virus, injury from falling down.The objective of the invention is, according to Chinese medical theory and treatment experience, in conjunction with modern chemistry and pharmacology's means, extraction separation obtains efficiently, nontoxic from Herba Centellae, can be used as the saponins compound of mental sickness such as treatment depression etc., i.e. asiatic centella total saponins.
The extraction process flow process of asiatic centella total saponins is as follows: take by weighing the herb of Herba Centellae, add 40%～80% ethanol and in the ultrasonic extraction device, extract, with extracting solution concentrate concentrated solution; Utilize cavitation suspension extraction segregation apparatus to use earlier chloroform extraction again, reclaim chloroform aqueous solution is partly used ethyl acetate extraction, then reclaim ethyl acetate and, reclaim n-butyl alcohol aqueous solution part reuse n-butanol extraction, drying promptly obtains the asiatic centella total saponins of high level.
The present invention has carried out the evaluation experimental of antidepressant effect to asiatic centella total saponins.
1, experiment purpose:
Inquire into the antidepressant effect mechanism of asiatic centella total saponins.
2, experimental technique:
Adopt several animal models such as the enhancing of Yohimbine toxicity is tested, apomorphine causes the stereotyped movement experiment to test the possibility mechanism of inquiring into the asiatic centella total saponins antidepressant effect
3, experiment content:
3.1 Yohimbine toxicity strengthens experiment give Yohimbine after, high dose is dead 1 in the 30min, in the 1h, medicine is high, medium and low, positive and blank each dead 1, the positive drug group is all dead in the 2h; The 24h mortality rate sees Table 1.
Table 1 asiatic centella total saponins to the influence of the lethal effect of Yohimbine (x ± s, n=10)
3.2 apomorphine causes mice stereotyped movement laboratory animal grouping administration, positive drug flupentixol and melitracen tablet amounts is 0.2mgkg
-1Each organizes mice behind last administration 1h, subcutaneous injection apomorphine 16mgkg
-1, put into the little mouse cage of 16 * 27cm immediately separately, the cage bottom is spread in advance and is rubbed crisp paper, and gauffer is upwards.According to the standards of grading of mice stereotyped movement, observe the degree that stereotyped movement appears in the interior animal of apomorphine 1h, see Table 2.
Table 2 asiatic centella total saponins to apomorphine cause stereotyped movement influence (x ± s, n=10)
Annotate: compare with the blank group,
bP＜0.05.
4, experimental result:
The result shows that asiatic centella total saponins is at 30～120mgkg
-1In the dosage range Yohimbine toxicity there is not potentiation.Asiatic centella total saponins does not increase the ability of mechanical effect to the mice of injection behind the apomorphine.
5, experiment conclusion:
More than experiment shows: it is evident in efficacy and side effect is little that the effect characteristics of compositions such as asiatic centella total saponins just are that it improves spirit depressing.
The specific embodiment:
Embodiment 1
The dry herb of 500g Herba Centellae, extract at the ultrasonic extraction device with 65% ethanol, extract three times, consumption is respectively 10,8,6 times, the each extraction 1 hour, extract the filtrate merging with three times and be concentrated into nothing alcohol, utilize cavitation suspension extraction segregation apparatus to use the chloroform of triplication respectively, ethyl acetate and n-butyl alcohol extract successively, reclaim solvent, drying gets product 34.5g, saponin content 18.0%.
Embodiment 2
The dry herb of 500g Herba Centellae, extract at the ultrasonic extraction device with 75% ethanol, extract three times, consumption is respectively 10,8,6 times, the each extraction 1 hour, extract the filtrate merging with three times and be concentrated into nothing alcohol, utilize cavitation suspension extraction segregation apparatus to use the chloroform of triplication respectively, ethyl acetate and n-butyl alcohol extract successively, reclaim solvent, drying gets product 22.6g, saponin content 39.6%.
Embodiment 3
The dry herb of 500g Herba Centellae, extract in the ultrasonic extraction device with 85% ethanol, extract three times, consumption is respectively 10,8,6 times, the each extraction 1 hour, extract the filtrate merging with three times and be concentrated into nothing alcohol, utilize cavitation suspension extraction segregation apparatus to use the chloroform of triplication respectively, ethyl acetate and n-butyl alcohol extract successively, reclaim solvent, drying gets product 17.5g, saponin content 68.5%.
Embodiment 4
Get asiatic centella total saponins 100g, disperse with an amount of water for injection, add right amount of auxiliary materials, mixing is made granule, drying.
Press the granule of embodiment gained, be sub-packed in the hungry area shell, promptly get capsule by required dosage.
Press the granule of embodiment gained, direct compression, film coating promptly gets tablet.
Be used for the treatment of mental sickness such as depression.
Embodiment 5
Asiatic centella total saponins is added the injection water in right amount disperse, spray drying or vacuum drying get asiaticoside dry powder.Taking polyethylene glycol 400 adds asiaticoside dry powder, and the limit edged is stirred to even, adopts pressing, makes soft capsule.Be used for the treatment of mental sickness such as depression.
Embodiment 6
Get asiatic centella total saponins 400mg, add the injection water and dissolve in right amount.With mannitol 400mg,, promptly get the asiatic centella total saponins lyophilized powder according to the lyophilizing of normal freeze-drying technology.Be used for the treatment of mental sickness such as depression.
Claims (8)
1, a kind of from Herba Centellae the method for extraction separation asiatic centella total saponins, it is characterized in that dry Herba Centellae Centella asiatica (L.) Urb herb is dipped in 40%～80% ethanol, extract with ultrasonic wave extraction, extracting solution concentrates, concentrated solution; Utilize cavitation suspension extraction equipment elder generation chloroform extraction again, water intaking part reuse ethyl acetate extraction, water intaking part is again used at last n-butanol extraction, gets n-butyl alcohol and partly carries out reduced-pressure backflow, and drying promptly gets asiatic centella total saponins.
2, by the herb of the described Herba Centellae of claim 1, it is characterized in that: Herba Centellae derives from the dry herb of samphire Herba Centellae Centella asiatica (L.) Urb..
3, extract with ultrasonic wave extraction by claim 1 is described, it is characterized in that the judder that utilizes ultrasound wave to produce, high acceleration, intensive cavitation effect, stirring action etc., the effective ingredient that quickens the Herba Centellae herb enters ethanol, so just improved extraction rate, shortened extraction time, and avoided extracting, and contained more impurity in the extracting solution that causes because of long-time.
4, by the described cavitation suspension extraction of claim 1 equipment, it is characterized in that utilizing novel a kind of simple in structure, quick mass transfer, sharp separation, extraction yield reach more than 95%, in the waste residue dissolvent residual at the negative pressure cavitation liquid extract and separate device below 5%.
5, described by claim 1 with chloroform and ethyl acetate extraction, it is characterized in that removing the composition of non-asiaticoside.
6,, it is characterized in that needing to reclaim solvent by described chloroform of claim 1 and ethyl acetate extraction part liquid.
7, by the described asiatic centella total saponins of claim 1, it is characterized in that including the asiaticoside compounds.
8, the application that can be used to prepare antidepressant drug by the described asiatic centella total saponins of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910152717A CN101653465A (en) | 2009-09-15 | 2009-09-15 | Extraction method of total saponin of asiatica and application thereof in medical field |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910152717A CN101653465A (en) | 2009-09-15 | 2009-09-15 | Extraction method of total saponin of asiatica and application thereof in medical field |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101653465A true CN101653465A (en) | 2010-02-24 |
Family
ID=41708065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910152717A Pending CN101653465A (en) | 2009-09-15 | 2009-09-15 | Extraction method of total saponin of asiatica and application thereof in medical field |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101653465A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102988434A (en) * | 2012-11-28 | 2013-03-27 | 深圳市海普瑞药业股份有限公司 | Centella effective extractive polyethylene glycol ointment and preparation method thereof |
| CN103393733A (en) * | 2013-08-19 | 2013-11-20 | 广西中医药大学 | Centella asiatica (l.) urban effective fraction and application thereof |
| CN106176848A (en) * | 2016-08-25 | 2016-12-07 | 桂林益天成生物科技有限公司 | The method extracting asiatic centella total saponins from Herba Centellae |
-
2009
- 2009-09-15 CN CN200910152717A patent/CN101653465A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102988434A (en) * | 2012-11-28 | 2013-03-27 | 深圳市海普瑞药业股份有限公司 | Centella effective extractive polyethylene glycol ointment and preparation method thereof |
| CN102988434B (en) * | 2012-11-28 | 2014-04-23 | 深圳市海普瑞药业股份有限公司 | Centella effective extractive polyethylene glycol ointment and preparation method thereof |
| CN103393733A (en) * | 2013-08-19 | 2013-11-20 | 广西中医药大学 | Centella asiatica (l.) urban effective fraction and application thereof |
| CN106176848A (en) * | 2016-08-25 | 2016-12-07 | 桂林益天成生物科技有限公司 | The method extracting asiatic centella total saponins from Herba Centellae |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103040924B (en) | Radix millettiae speciosae extract and application thereof in preparing drugs for treating osteoporosis | |
| CN100577134C (en) | Super micronization method for botanical medicinal, product obtained thereby and use thereof | |
| CN101401851A (en) | Method for extracting rhubarb total dihydrodiketoanthracene from rhubarb | |
| CN1530112A (en) | Medicine for preventing fibrous liver and preparing method thereof | |
| CN101049354A (en) | Method for extracting effective constituent in raw material of hawthorn | |
| CN105147618A (en) | Preparation method for Chinese herb decocting-free preparation containing heat-sensitive effective components | |
| CN101653465A (en) | Extraction method of total saponin of asiatica and application thereof in medical field | |
| CN103641821A (en) | Preparation method of milk thistle extract with high content and low soluble residue | |
| CN101401843A (en) | Extracting method for active principle of traditional Chinese medicine red sage root | |
| CN102091107B (en) | Method for extracting total triterpene of centella asiatica by enzymic process | |
| CN1155592C (en) | Process for preparing total jujuboflavone with calmative and hypnosis action | |
| CN112972527A (en) | Fengliaochangweikang tablet and its extraction process | |
| CN105943576A (en) | Extracting method of radix acanthopanacis semticosi and radix acanthopanacis semticosi health product | |
| CN103006782B (en) | Ficus auriculata extract and application thereof in preparation of medicine used for treating osteoporosis | |
| CN111195289A (en) | Capsule containing extracts of Ganoderma, Ganoderma spore powder and fructus Ligustri Lucidi, and its preparation method | |
| CN104367888A (en) | Medicine for treating acute and chronic balanitis | |
| CN104306519A (en) | Anti-oxidation composite traditional Chinese medicine extraction isolate and preparation method thereof | |
| CN102114063B (en) | Method for extracting and separating asiaticoside by countercurrent extraction combined with membrane separation | |
| CN102824550A (en) | Method for preparing compound gastrodia elata preparation | |
| CN101172137A (en) | Method for high effectively extracting pseudo-purslane saponin by using cavitated suspension extraction and stripping apparatus | |
| CN105148240A (en) | Medicinal preparation used for treating chronic cardiac failure | |
| CN1660379A (en) | Combination of medication, preparing method and method of controlling quality | |
| CN1857378A (en) | Compound wild kiwi tablet and its preparing method | |
| CN100571728C (en) | A kind of method for preparing WANTONG JINGU PIAN | |
| CN105288504A (en) | Pharmaceutical preparation for treating dysmenorrhea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100224 |