CN101649335A - Preparation method of high-purity alpha-1,6 trisaccharide - Google Patents
Preparation method of high-purity alpha-1,6 trisaccharide Download PDFInfo
- Publication number
- CN101649335A CN101649335A CN200910087441A CN200910087441A CN101649335A CN 101649335 A CN101649335 A CN 101649335A CN 200910087441 A CN200910087441 A CN 200910087441A CN 200910087441 A CN200910087441 A CN 200910087441A CN 101649335 A CN101649335 A CN 101649335A
- Authority
- CN
- China
- Prior art keywords
- ratio
- gala
- trisaccharide
- juice
- purity alpha
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a preparation method of high-purity alpha-1,6 gala-trisaccharide, which comprises the following steps: (1)juicing: lixiviating dried Chinese ortichoke or Yinbai in water and juicing; (2) fermentation: mixing the juice, yeast cream, MgSO47H2O and KH2PO4, sterilizing, and then cooling to reach the normal temperature, adding dried yeast for standing and culturing at the constant temperature of 28-37 DEG C to obtain fermentation liquor;(3) impurity removal; (4) decolorization; (5) desalination; and (6) concentration and drying, thus, high-purity alpha-1,6 gala-trisaccharideis obtained. In the invention, a microbe fermentation method is adopted to extract high-purity alpha-1,6 gala-trisaccharide from natural plants, thereby laying a foundation for the functional research of alpha-1,6 gala-trisaccharide with high purity and efficiency; the monosaccharide content is lower than 3 5, thereby further widening the application fields and target people of the alpha-1,6 gala-trisaccharide.
Description
Technical field
The present invention relates to the preparation method of a kind of α-1,6 gala trisaccharide.
Background technology
α-1,6 gala trisaccharide is the main component in the Radix Rehmanniae Preparata oligose, has another name called manninotriose, mannotriose (being different from the mannotriose in the manna oligosaccharide), English name manninotriose.α-1,6 gala trisaccharide structure is clear and definite, molecular formula C
18H
32O
16, molecular weight 504, it connects two molecule semi-lactosi polymerizations by glucose with α-1,6 glycosidic link and forms, and structure is D-Gal-alpha1-＞6D-Gal-alpha1-＞6D-Glucose, is a kind of of oligomeric galactose.
α-1,6 gala trisaccharide is a kind of as oligomeric galactose, and very high utility value is arranged.Domestic research and development to α-1,6 gala trisaccharide at present are less, do not see the research report and the relevant patent of high-purity α-1,6 gala trisaccharide.Read up the literature " preparation of mannotriose and process optimization thereof " (military defense is red etc., " Chinese medicinal materials ") in the mannotriose preparation technology that mentions be the acid-hydrolysis method of stachyose, under pH was 2.5,90 ℃ and 24h condition, 90.63% stachyose hydrolysis became mannotriose and fructose.Document " activeconstituents mannotriose Study on extraction in the Radix Rehmanniae Preparata " (Guo Wei etc., " Shanghai Univ. of Traditional Chinese Medicine's journal ") employing Radix Rehmanniae Preparata is a raw material, and boiling water lixiviate 6h adds 4 times of water, extracts 2 times, and the mannotriose content that obtains is 25.4%.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, the preparation method of a kind of high-purity alpha-1,6 gala trisaccharide is provided.
Technical scheme of the present invention is summarized as follows:
The preparation method of a kind of high-purity alpha-1,6 gala trisaccharide, be made up of following steps:
(1) squeezes the juice: be 1: 3～6 ratio in mass ratio, drying stachys sieboldii miq or argentate strip are added water in 60 ℃～90 ℃ lixiviates 0.5 hour～1.5 hours, squeeze the juice, get residue, add 1 times～3 times 0.5 hour～1.5 hours multiple press for extracting juicees of 60 ℃～90 ℃ lixiviates of water, mix twice and get squeezeding juice to the residue quality;
(2) fermentation: in mass ratio is 1000: (5～10): (0.05～0.5): the ratio of (0.4～1), and with squeezeding juice, yeast extract paste, MgSO
47H
2O and KH
2PO
4Mix, sterilization is cooled to behind the normal temperature in the ratio of 1L: 0.5～2.0g, adds the good dry yeast of activation in mixed solution, and 28～37 ℃ of constant temperature leave standstill to be cultivated 2 hours～8 hours, stopped fermentation, fermented liquid;
(3) removal of impurities: described fermented liquid is warming up to 95～130 ℃, kept 5～30 minutes, filter, disgorging joins finings calcium hydroxide in the filtrate by the ratio of 5～12g: 1L, treats that precipitation is complete, gets supernatant liquor;
(4) decolouring: in the ratio of 1L: 4～10g, in supernatant liquor, add gac, be heated to 60～90 ℃, decoloured 30～60 minutes, remove by filter gac;
(5) desalination: the liquid after will decolouring passes through Zeo-karb, anionite-exchange resin successively, obtains refining liquid glucose;
(6) concentrated and dry: with described refining liquid glucose filtering with microporous membrane, at 45～75 ℃ of vacuum concentration, drying obtains a kind of high-purity alpha-1,6 gala trisaccharide.
Step (1) is preferably: in mass ratio is 1: 4～5 ratio, and drying stachys sieboldii miq or argentate strip are added water in 70 ℃～80 ℃ lixiviates 1 hour, squeezes the juice, and gets residue, adds 2 times of 1 hour multiple press for extracting juicees of 70 ℃～80 ℃ lixiviates of water to the residue quality, and mixing twice must squeezeding juice.
Step (2) is preferably: in mass ratio is 1000: (7～8): (0.1～0.2): the ratio of (0.6～0.8), and with squeezeding juice, yeast extract paste, MgSO
47H
2O and KH
2PO
4Mix, sterilization is cooled to behind the normal temperature in the ratio of 1L: 1.0g, adds the good dry yeast of activation in mixed solution, and 30～32 ℃ of constant temperature leave standstill to be cultivated 4 hours～6 hours, stopped fermentation, fermented liquid.
Step (3) is preferably: described fermented liquid is warming up to 120 ℃, kept 8 minutes, filter, disgorging joins finings calcium hydroxide in the filtrate by the ratio of 8g: 1L, treats that precipitation is complete, gets supernatant liquor.
Step (4) is preferably: in the ratio of 1L: 6～8g, add gac in supernatant liquor, be heated to 70～80 ℃, decoloured 40～50 minutes, remove by filter gac.
Characteristics of the present invention:
1. the present invention adopts microbe fermentation method directly to extract high-purity alpha-1,6 gala trisaccharide from natural phant, for the functional study of α-1,6 gala trisaccharide is laid a good foundation.
2. by to effective control of fermenting process, α in the liquid glucose that obtains-1,6 gala trisaccharide purity be higher than 90% and the stachyose rate of decomposition greater than 98%, yield is higher.
3. mono-and di-saccharides content is lower than 3%, has further enlarged the Application Areas of α-1,6 gala trisaccharide and has benefited from the crowd.
4. improve the utility value of raw materials such as Rhizome of Bear's-foot Fern, argentate strip, increased farmers' income, created high social.
Description of drawings
Fig. 1 is the HPLC figure of the preceding squeezeding juice of fermentation.
Fig. 2 is the HPLC figure of the refining liquid glucose in fermentation back.
Embodiment
The present invention extracts stachyose with water as solvent from plant, the component of various solubilities is all dissolved in the water in the plant, comprises various carbohydrates (monose, disaccharide, trisaccharide, tetrose etc.) and soluble protein, various organic acid, inorganic salt etc.Adopt fermentation method that the stachyose in the extracting solution is decomposed into α-1 then, 6 gala trisaccharide and fructose, and the single, double sugar consumption in the liquid glucose fallen, by precipitation, decolouring, ion-exchange, membrane filtration etc., protein, organic acid, inorganic salt content are dropped to minimum level, obtain a kind of α-1,6 gala trisaccharide content (accounts for the total reducing sugar quality) about 90% product.
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
The preparation method of a kind of high-purity alpha-1,6 gala trisaccharide, be made up of following steps:
(1) squeezes the juice: the ratio that in mass ratio is 1: 4, drying stachys sieboldii miq is added water in 70 ℃ of lixiviates 1 hour, squeeze the juice, get residue, add 2 times of 1 hour multiple press for extracting juicees of 70 ℃ of lixiviates of water, mix twice to such an extent that squeezeding juice (the HPLC figure of squeezeding juice sees Fig. 1) adjustment soluble solid content is 10 ° of Brix to the residue quality;
(2) fermentation: in mass ratio is 1000: 7: 0.1: 0.6 ratio, and with squeezeding juice, yeast extract paste, MgSO
47H
2O and KH
2PO
4Mix, sterilization is cooled to behind the normal temperature in the ratio of 1L: 1.0g, adds the good dry yeast of activation in mixed solution, and 30 ℃ of constant temperature leave standstill to be cultivated 6 hours, stopped fermentation, fermented liquid;
(3) removal of impurities: described fermented liquid is warming up to 100 ℃, kept 20 minutes, filter, disgorging joins finings calcium hydroxide in the filtrate by the ratio of 8g: 1L, treats that precipitation is complete, gets supernatant liquor;
(4) decolouring: in the ratio of 1L: 6g, in supernatant liquor, add gac, be heated to 80 ℃, decoloured 40 minutes, remove by filter gac;
(5) desalination: the liquid after will decolouring passes through Zeo-karb, anionite-exchange resin successively, obtains refining liquid glucose (the HPLC figure of refining liquid glucose sees Fig. 2);
(6) concentrate and dry: with described refining liquid glucose filtering with microporous membrane, at 50 ℃ of vacuum concentration, drying obtains a kind of purity and is 90% high-purity alpha-1,6 gala trisaccharide.
The sugar (quality percentage composition) of the refining liquid glucose of squeezeding juice and fermentation back before the fermentation
| Sugar/% | Fructose | Glucose | Sucrose | Maltose | Melibiose | Raffinose | The gala trisaccharide | Stachyose | Verbascose |
| Before the fermentation | - | ??0.78 | ??11.9 | ??0.54 | ??- | ??4.68 | ??0.63 | ??74.6 | ??2.12 |
| After the fermentation | 0.64 | ??1.38 | ??2.09 | ??0.60 | ??4.92 | ??- | ??90.09 | ??- | ??- |
Embodiment 2
The preparation method of a kind of high-purity alpha-1,6 gala trisaccharide, be made up of following steps:
(1) squeeze the juice: in mass ratio is 1: 5 ratio, and dry argentate strip is added water in 80 ℃ of lixiviates 1 hour, squeezes the juice, get residue, add 2 times of 1 hour multiple press for extracting juicees of 80 ℃ of lixiviates of water to the residue quality, mix twice and get squeezeding juice, adjusting soluble solid content is 11 ° of Brix;
(2) fermentation: in mass ratio is 1000: 8: 0.2: 0.8 ratio, and with squeezeding juice, yeast extract paste, MgSO
47H
2O and KH
2PO
4Mix, sterilization is cooled to behind the normal temperature in the ratio of 1L: 1.0g, adds the good dry yeast of activation in mixed solution, and 32 ℃ of constant temperature leave standstill to be cultivated 4 hours, stopped fermentation, fermented liquid;
(3) removal of impurities: described fermented liquid is warming up to 110 ℃, kept 15 minutes, filter, disgorging joins finings calcium hydroxide in the filtrate by the ratio of 8g: 1L, treats that precipitation is complete, gets supernatant liquor;
(4) decolouring: in the ratio of 1L: 8g, in supernatant liquor, add gac, be heated to 70 ℃, decoloured 50 minutes, remove by filter gac;
(5) desalination: the liquid after will decolouring passes through Zeo-karb, anionite-exchange resin successively, obtains refining liquid glucose;
(6) concentrate and dry: with described refining liquid glucose filtering with microporous membrane, at 60 ℃ of vacuum concentration, drying obtains a kind of purity and is 91% high-purity alpha-1,6 gala trisaccharide.
Embodiment 3
The preparation method of a kind of high-purity alpha-1,6 gala trisaccharide, be made up of following steps:
(1) squeeze the juice: in mass ratio is 1: 3 ratio, and drying stachys sieboldii miq is added water in 90 ℃ of lixiviates 0.5 hour, squeezes the juice, get residue, add 1 times of 0.5 hour multiple press for extracting juice of 90 ℃ of lixiviates of water to the residue quality, mix twice and get squeezeding juice, adjusting soluble solid content is 9 ° of Brix;
(2) fermentation: in mass ratio is 1000: 5: 0.05: 0.4 ratio, and with squeezeding juice, yeast extract paste, MgSO
47H
2O and KH
2PO
4Mix, sterilization is cooled to behind the normal temperature in the ratio of 1L: 0.5g, adds the good dry yeast of activation in mixed solution, and 28 ℃ of constant temperature leave standstill to be cultivated 8 hours, stopped fermentation, fermented liquid;
(3) removal of impurities: described fermented liquid is warming up to 120 ℃, kept 10 minutes, filter, disgorging joins finings calcium hydroxide in the filtrate by the ratio of 5g: 1L, treats that precipitation is complete, gets supernatant liquor;
(4) decolouring: in the ratio of 1L: 4g, in supernatant liquor, add gac, be heated to 60 ℃, decoloured 60 minutes, remove by filter gac;
(5) desalination: the liquid after will decolouring passes through Zeo-karb, anionite-exchange resin successively, obtains refining liquid glucose;
(6) concentrate and dry: with described refining liquid glucose filtering with microporous membrane, at 45 ℃ of vacuum concentration, drying obtains a kind of purity and is 93% high-purity alpha-1,6 gala trisaccharide.
The preparation method of a kind of high-purity alpha-1,6 gala trisaccharide, be made up of following steps:
(1) squeeze the juice: in mass ratio is 1: 6 ratio, and dry argentate strip is added water in 60 ℃ of lixiviates 1.5 hours, squeezes the juice, get residue, add 3 times of 1.5 hours multiple press for extracting juicees of 60 ℃ of lixiviates of water to the residue quality, mix twice and get squeezeding juice, adjusting soluble solid content is 12 ° of Brix;
(2) fermentation: in mass ratio is 1000: 10: 0.5: 1 ratio, and with squeezeding juice, yeast extract paste, MgSO
47H
2O and KH
2PO
4Mix, sterilization is cooled to behind the normal temperature in the ratio of 1L: 2.0g, adds the good dry yeast of activation in mixed solution, and 37 ℃ of constant temperature leave standstill to be cultivated 2 hours, stopped fermentation, fermented liquid;
(3) removal of impurities: described fermented liquid is warming up to 130 ℃, kept 5 minutes, filter, disgorging joins finings calcium hydroxide in the filtrate by the ratio of 12g: 1L, treats that precipitation is complete, gets supernatant liquor;
(4) decolouring: in the ratio of 1L: 10g, in supernatant liquor, add gac, be heated to 90 ℃, decoloured 30 minutes, remove by filter gac;
(5) desalination: the liquid after will decolouring passes through Zeo-karb, anionite-exchange resin successively, obtains refining liquid glucose;
(6) concentrate and dry: with described refining liquid glucose filtering with microporous membrane, at 75 ℃ of vacuum concentration, drying obtains a kind of purity and is 92% high-purity alpha-1,6 gala trisaccharide.
The preparation method of a kind of high-purity alpha-1,6 gala trisaccharide, be made up of following steps:
(1) squeeze the juice: add 80 ℃ of hot dippings of 4L water after 1000g hay cadbait is cleaned and carry 1 hour, get residue after squeezing the juice and add 2 times of 80 ℃ of hot dippings of water and carry 1 hour multiple press for extracting juice to the residue quality, mixes squeeze the juice for twice squeezeding juice, the adjustment soluble solid content is 10 ° of Brix;
(2) fermentation: in mass ratio is 1000: 10: 0.5: 1 ratio, and with squeezeding juice, yeast extract paste, MgSO
47H
2O and KH
2PO
4Mix, sterilization is cooled to behind the normal temperature in the ratio of 1L: 0.5g, adds the good dry yeast of activation in mixed solution, and 28 ℃ of constant temperature leave standstill to be cultivated 2 hours, stopped fermentation, fermented liquid;
(3) removal of impurities: described fermented liquid is warming up to 95 ℃, kept 30 minutes, filter, disgorging joins finings calcium hydroxide in the filtrate by the ratio of 12g: 1L, treats that precipitation is complete, gets supernatant liquor;
(4) decolouring: in the ratio of 1L: 10g, in supernatant liquor, add gac, be heated to 80 ℃, decoloured 30 minutes, remove by filter gac;
(5) desalination: the liquid after will decolouring passes through Zeo-karb, anionite-exchange resin successively, obtains refining liquid glucose;
(6) concentrate and dry: with described refining liquid glucose filtering with microporous membrane, at 75 ℃ of vacuum concentration, drying obtains a kind of purity and is 93% high-purity alpha-1,6 gala trisaccharide.
Claims (5)
1. the preparation method of high-purity alpha-1,6 a gala trisaccharide is characterized in that being made up of following steps:
(1) squeezes the juice: be 1: 3～6 ratio in mass ratio, drying stachys sieboldii miq or argentate strip are added water in 60 ℃～90 ℃ lixiviates 0.5 hour～1.5 hours, squeeze the juice, get residue, add 1 times～3 times 0.5 hour～1.5 hours multiple press for extracting juicees of 60 ℃～90 ℃ lixiviates of water, mix twice and get squeezeding juice to the residue quality;
(2) fermentation: in mass ratio is 1000: (5～10): (0.05～0.5): the ratio of (0.4～1), and with squeezeding juice, yeast extract paste, MgSO
47H
2O and KH
2PO
4Mix, sterilization is cooled to behind the normal temperature in the ratio of 1L: 0.5～2.0g, adds the good dry yeast of activation in mixed solution, and 28～37 ℃ of constant temperature leave standstill to be cultivated 2 hours～8 hours, stopped fermentation, fermented liquid;
(3) removal of impurities: described fermented liquid is warming up to 95～130 ℃, kept 5～30 minutes, filter, disgorging joins finings calcium hydroxide in the filtrate by the ratio of 5～12g: 1L, treats that precipitation is complete, gets supernatant liquor;
(4) decolouring: in the ratio of 1L: 4～10g, in supernatant liquor, add gac, be heated to 60～90 ℃, decoloured 30～60 minutes, remove by filter gac;
(5) desalination: the liquid after will decolouring passes through Zeo-karb, anionite-exchange resin successively, obtains refining liquid glucose;
(6) concentrated and dry: with described refining liquid glucose filtering with microporous membrane, at 45～75 ℃ of vacuum concentration, drying obtains a kind of high-purity alpha-1,6 gala trisaccharide.
2. a kind of high-purity alpha-1 according to claim 1, the preparation method of 6 gala trisaccharides, it is characterized in that described step (1) is: be 1: 4～5 ratio in mass ratio, drying stachys sieboldii miq or argentate strip are added water in 70 ℃～80 ℃ lixiviates 1 hour, squeeze the juice, get residue, add 2 times of 1 hour multiple press for extracting juicees of 70 ℃～80 ℃ lixiviates of water, mix twice and get squeezeding juice to the residue quality.
3. the preparation method of a kind of high-purity alpha-1,6 gala trisaccharide according to claim 1, it is characterized in that described step (2) is: in mass ratio is 1000: (7～8): (0.1～0.2): the ratio of (0.6～0.8), with squeezeding juice, yeast extract paste, MgSO
47H
2O and KH
2PO
4Mix, sterilization is cooled to behind the normal temperature in the ratio of 1L: 1.0g, adds the good dry yeast of activation in mixed solution, and 30～32 ℃ of constant temperature leave standstill to be cultivated 4 hours～6 hours, stopped fermentation, fermented liquid.
4. a kind of high-purity alpha-1 according to claim 1, the preparation method of 6 gala trisaccharides, it is characterized in that described step (3) is: described fermented liquid is warming up to 120 ℃, kept 8 minutes, filter, disgorging joins finings calcium hydroxide in the filtrate in the ratio of 8g: 1L, treat that precipitation is complete, get supernatant liquor.
5. the preparation method of a kind of high-purity alpha-1,6 gala trisaccharide according to claim 1 is characterized in that described step (4) is: in the ratio of 1L: 6～8g, in supernatant liquor, add gac, be heated to 70～80 ℃, decoloured 40～50 minutes, remove by filter gac.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100874419A CN101649335B (en) | 2009-06-25 | 2009-06-25 | Preparation method of high-purity alpha-1,6 trisaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100874419A CN101649335B (en) | 2009-06-25 | 2009-06-25 | Preparation method of high-purity alpha-1,6 trisaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101649335A true CN101649335A (en) | 2010-02-17 |
| CN101649335B CN101649335B (en) | 2012-02-15 |
Family
ID=41671661
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100874419A Active CN101649335B (en) | 2009-06-25 | 2009-06-25 | Preparation method of high-purity alpha-1,6 trisaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101649335B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103131744A (en) * | 2013-02-04 | 2013-06-05 | 中国食品发酵工业研究院 | Preparation method of high-purity plant source oligomerization galactose |
| CN104059111A (en) * | 2014-05-19 | 2014-09-24 | 邱建国 | Method for extracting manninotriose monomer from rehmannia by virtue of activated carbon gradient elution |
| CN108836974A (en) * | 2018-05-23 | 2018-11-20 | 宁波拜尔玛生物科技有限公司 | Hypoglycemic and liver-protecting combination based on multipath adjustment mechanism |
| CN111184172A (en) * | 2020-03-02 | 2020-05-22 | 兰州大学 | Codonopsis pilosula oligosaccharide solid beverage and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100434434C (en) * | 2004-07-01 | 2008-11-19 | 西安大鹏生物科技股份有限公司 | Method for extracting high purity stachyose from radix rehmanniae recen and producing prepared rehmannia root |
-
2009
- 2009-06-25 CN CN2009100874419A patent/CN101649335B/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103131744A (en) * | 2013-02-04 | 2013-06-05 | 中国食品发酵工业研究院 | Preparation method of high-purity plant source oligomerization galactose |
| CN104059111A (en) * | 2014-05-19 | 2014-09-24 | 邱建国 | Method for extracting manninotriose monomer from rehmannia by virtue of activated carbon gradient elution |
| CN108836974A (en) * | 2018-05-23 | 2018-11-20 | 宁波拜尔玛生物科技有限公司 | Hypoglycemic and liver-protecting combination based on multipath adjustment mechanism |
| CN108836974B (en) * | 2018-05-23 | 2020-09-29 | 宁波拜尔玛生物科技有限公司 | Composition for reducing blood sugar and protecting liver based on multipath regulation mechanism |
| CN111184172A (en) * | 2020-03-02 | 2020-05-22 | 兰州大学 | Codonopsis pilosula oligosaccharide solid beverage and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101649335B (en) | 2012-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102286571B (en) | Clean and high-efficiency production process for preparing xylose and L-arabinose | |
| CN101736045B (en) | Method for continuously extracting functional components of chlorella vulgaris | |
| CN102504048B (en) | Jerusalem artichoke/chicory comprehensive utilization method | |
| CN101649286B (en) | Method for preparing hawthorn fruit vinegar | |
| CN103642876B (en) | The method of high-purity chicory, jerusalem artichoke type oligofructose co-production polyfructosan | |
| CN102876732B (en) | Method for preparing high-added-value sugar alcohols by efficiently using wood fiber raw materials | |
| CN102406048B (en) | Method for preparing sea cucumber glycoprotein by using sea cucumber blanching solution | |
| CN106086116A (en) | A kind of trehalose preparation method | |
| CN1300857A (en) | Stachyose and its preparing process | |
| CN101649335B (en) | Preparation method of high-purity alpha-1,6 trisaccharide | |
| CN106901363A (en) | A kind of preparation method of olive pomace ferment | |
| CN106912964A (en) | Soluble dietary fiber and preparation method thereof | |
| CN101829284B (en) | Process for extracting and refining flavone in highland barley seedlings by enzymolysis-membrane ultrafiltration | |
| CN106349398A (en) | Cichorium intybus inulin extracting method | |
| CN102850410A (en) | Method for extracting seaweed polysaccharide from waste beer yeast | |
| CN101597635B (en) | Method for preparing high purity stachyose | |
| CN106947000B (en) | A kind of preparation method of high-purity polyfructosan | |
| CN113402626A (en) | Nymphaea hybrid polysaccharide extract and preparation method and application thereof | |
| CN104069191A (en) | Extraction process of total flavonoids of polygonum criopolitanum | |
| CN104762333B (en) | Method for preparing xylitol by utilizing winter bamboo shoot shells | |
| CN1927946A (en) | Extraction method of natural plant water-soluble red colouring matter | |
| CN105296545A (en) | Method for converting polydatin through microorganism to prepare resveratrol | |
| CN109576328A (en) | A kind of preparation method of asparagus agaropectin oligose | |
| CN102070677B (en) | Method for refining raffinose | |
| CN105315314A (en) | Industrial production method for extracting stachyose from radix rehmanniae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |