CN101632387B - Composition preparation of cuaminosulfate and anilazine for resisting tobacco rust - Google Patents
Composition preparation of cuaminosulfate and anilazine for resisting tobacco rust Download PDFInfo
- Publication number
- CN101632387B CN101632387B CN2009100863147A CN200910086314A CN101632387B CN 101632387 B CN101632387 B CN 101632387B CN 2009100863147 A CN2009100863147 A CN 2009100863147A CN 200910086314 A CN200910086314 A CN 200910086314A CN 101632387 B CN101632387 B CN 101632387B
- Authority
- CN
- China
- Prior art keywords
- hymexazo
- cuaminosulfate
- network ammonia
- composition preparation
- anilazine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a composition preparation of cuaminosulfate for resisting tobacco rust, comprising cuaminosulfate, anilazine and water, wherein the weight proportioning range of the cuaminosulfate and the anilazine is 1:1-5, the optimal proportion is 1:2.17, and the form of the composition preparation is aqueous solution. The composition preparation of cuaminosulfate for resisting tobacco rust is used for preventing tobacco rust and has high efficiency, low toxicity, low cost and no environmental pollution.
Description
Technical field
The present invention relates to the tobacco agricultural chemicals, particularly a kind of network ammonia Tong is Yu the anti-Alternaria alternate combination agent of hymexazo.
Background technology
Tobacco is one of important economic crops of China, and there are 26 province plantation tobaccos in China, and wherein province's area under cultivations such as Yunnan, Guizhou, Henan, Shandong, Anhui, Hunan, Hubei are bigger.All there is certain cultivated area each province, the middle and lower reach of Yangtze River, and China's tobacco production occupies first place in the world.
It is reported that whole world tobacco diseases has kind more than 100, what China put down in writing has nearly 40 kinds.Wherein rust is that tobacco produces one of disease that goes up the harm maximum, and there are Shandong, Henan, Anhui, Heilungkiang, Jilin in the province of rust serious harm; Next is Sichuan, Yunnan, Guizhou, Liaoning, Shaanxi, and in addition, the harm of the red cigarette of the solarization district in the Turkish tobaccos producing region in Guangdong, Zhejiang is also heavier, accounts for the 30%-35% of national cultivated area according to the annual rust onset area of estimation.Rust is main with the blade morbidity, also endangers stem sometimes, and is generally serious in the cigarette strain sequela of pinching.After the cigarette strain morbidity, circular dark brown point appears earlier in the bottom leaf; Still can keep circular after scab enlarges, concentric wheel stripe appears in the dead tissue on the scab.The scab edge has yellow haloing to center on, and with health tissues obvious boundary is arranged, and produces dark brown or black powder in the scab, finally cause the incomplete grade of tobacco leaf to descend, and because interior quality is inharmonious.Raise like total nitrogen, protein, total reducing sugar, reducing sugar reduce, and candy alkali ratio descends, and makes jealous variation, greatly reduces industrial utility value, has become an important topic anxious to be solved on the cured tobacco production at present.China is to the control of Alternaria alternate at present, and main disease-resistant to plant (anti-disease) kind that adopts is master, reinforcement cultivation management, carries out comprehensive preventive health measuress such as crop rotation, the rational use of medicines.The chemical control kind is more single; Mainly contain 40% dimethachlon wetting powder (Si Peisi), 40% dimethachlon Cupravit wetting powder kinds such as (Chi Bante) on the market at present; Because these kinds have been used for many years, Alternaria alternate develops immunity to drugs to it, and the pulvis contaminated environment.
Summary of the invention
In order to reduce environmental pollution, to avoid the use of the pesticide resistance that conventional products causes, the present invention provides a kind of efficient, low toxicity, network ammonia Tong free from environmental pollution Yu the anti-Alternaria alternate combination agent of hymexazo.
The present invention is through to the compound screening of number of different types bactericide, and finding network ammonia Tong with hymexazo is composite has a notable synergistic effect.Hymexazo is a systemic fungicide; Can be absorbed to reach in root system by the root of plant, stem, leaf and move, produce two kinds of glucosides, crop had the effect that improves physiologically active at the plant intracellular metabolite; Thereby can promote plant strain growth, the activity of the branch evil of root, the increase of root hair and root improves.And network ammonia copper is mainly through copper ion performance bactericidal action; Cation exchange such as the K+ ion on copper ion and the pathogen surface of cell membrane, H+ ion; Make the protein coagulating on the pathogen cell membrane, simultaneously the part copper iontophoretic injection is gone in the pathogen cell and is combined with some enzyme, influences its activity; Network ammonia copper plays certain disease-resistant and production-increasing function to the certain facilitation of growth tool of cotton seedling, watermelon etc.
The invention provides a kind of network ammonia Tong Yu the anti-Alternaria alternate combination agent of hymexazo, according to synergy principle obvious, with low cost, wherein network ammonia Tong is with the ratio range of hymexazo is that network ammonia Tong is Yu the part by weight of hymexazo is 1: 1～5.
Wherein network ammonia Tong is Yu the ratio of hymexazo is 1: 2.17 o'clock, and synergy is best, and spending coefficient together is 252.
Network ammonia Tong of the present invention is Yu the formulation of the anti-Alternaria alternate combination agent of hymexazo is an aqua.
By pilot product quality of chemical products supervision and inspection station check through Beijing that the prescription of composition pesticide of the present invention is processed, the key technical indexes is all qualified.
Tobacco Institute, Chinese Academy of Agricultural Science's test of pesticide effectiveness shows, reaches 83.92% according to 7 days control efficiency after 1000 times of dispensers of present composition network ammonia copper 6%+ hymexazo 13% De hymexazo network ammonia copper aqua dilution, and control efficiency is outstanding.
Through Prevention and Cure Inst. of Occupational Diseaste of Labour and Hygiene, Shando composition pesticide rat acute of the present invention is tested through skin, acute oral, skin, eyes, sensitization; Show that this pesticide combination is a low toxicity; To rabbit skin nonirritant, the rabbit eyes are slight excitant, belong to weak sensitizer.Said preparation production, safe in utilization is described.
Network ammonia Tong of the present invention is Yu the anti-Alternaria alternate combination agent of hymexazo, efficient, the low toxicity, cheap of control Alternaria alternate, and free from environmental pollution.
Embodiment
Set forth network ammonia Tong of the present invention Yu the anti-Alternaria alternate combination agent of hymexazo is done more detailed explanation below in conjunction with embodiment for further.
The preparation of instance 1 19% hymexazo network ammonia copper aqua
Prescription:
Network ammonia copper: 6%;
Hymexazo: 13%;
Water: polishing to 100%.
Preparation method: take by weighing Shi Liang hymexazo and network ammonia copper by weight, put into agitator tank, add suitable quantity of water and be stirred to former medicine and dissolve fully and get final product.
The indoor prescription screening of instance 2 19% hymexazo network ammonia copper aquas report
For two kinds of medicaments of Ming Que hymexazo and network ammonia copper and different proportioning mixture thereof the preventive and therapeutic effect to Alternaria alternate, our factory is measured the indoor virulence of the important pathogenic bacteria Alternaria alternate of tobacco the network ammonia Tong 、 hymexazo and the preparation series that is mixed thereof.Growth rate method is adopted in test, calculates concentration (EC50) in the inhibition of each single agent and mixture, and the co-toxicity coefficient of different proportionings combination, and evaluation has been made in the synergy of different proportionings, result of the test is reported at present
As follows:
1. materials and methods
1.1 supply the examination material
Supply the examination germ: Alternaria alternate (biological and ecological methods to prevent plant disease, pests, and erosion group in the Chinese Academy of Agricultural Sciences Institute of Plant Protection provides)
Supply the reagent agent: handle A:23% network ammonia copper aqua: Beijing Dongwang Pesticide Factory's self-control;
Treatments B: the former medicine of 95% hymexazo: sky, Weifang, Shandong reaches plant protection Co., Ltd to be provided;
Handle C, D, E, F, G medicament! hymexazo: network ammonia copper=1: 18,6: 13,13: 6,15: 4,18: 1): Beijing Dongwang Pesticide Factory's self-control.
1.2 test method
1.2.1 growth rate method.
Adopt bacterium cake inoculation method, medium is potato agar medium (preparation method slightly).Test method is following: the medicament water is made into series concentration, presses 1: 9 mixed with the medium of fusing again, shake up.Get 10 milliliters of above-mentioned mixed medicine medium, the culture dish of putting into sterilization is the medium of band medicine.After the cooling, culture dish is inverted for use.In 27 ± 1 ℃ of incubators, cultivate, the uniform germ of growth plane, with the card punch of diameter 6mm break into diameter 6mm the bacterium cake several.Provoke the bacterium cake with transfer needle, bacterium is faced down to be placed on the ready-made band medicine medium.Put into 1 bacterium cake in each culture dish.The band medicine medium that adds the bacterium cake is inverted in the insulating box cultivates.Measure colony diameter after 5 days, calculate relative inhibition, toxicity regression formula, co-toxicity coefficient etc., each handles repetition 3 times.
1.2.2 the computational methods of inhibiting rate:
Relative inhibition=(contrast colony diameter-processing colony diameter)/(contrast colony diameter-6)
1.2.3 the computational methods of co-toxicity coefficient:
Ask by the abundant method of Sun Yun
2. result of the test
Test data and result's statistical method adopts probit value analytical method in the biostatistics, and drug dose is converted into logarithm value, and inhibiting rate becomes probit value, tries to achieve virulence regression equation, and uses X
2Carry out the test of significance of regression equation, calculate concentration (EC50) and 95% confidence limit thereof in the inhibition, obtain co-toxicity coefficient at last.
Result of the test is seen table 1
Table 1 network ammonia Tong 、 hymexazo and being mixed to the tobacco brown spot pathogen toxicity test
| Handle | Medicament and proportioning | Toxicity regression formula Y=a+bX | Concentration in the inhibition (EC50 μ g/g) | 95% confidence limit | Co-toxicity coefficient (CTC) |
| A | Network ammonia copper | Y＝0.29+1.83X | 368 | 471.5-286.8 | - |
| B | Hymexazo | Y＝1.96+1.38X | 156 | 241.0-114.4 | - |
| Hymexazo: network ammonia copper | |||||
| C | 1∶18 | Y＝3.47+0.68X | 159 | 290.6-87.30 | 216 |
| D | 6∶13 | Y＝2.22+1.16X | 242 | 351.9-166.2 | 108 |
| E | 13∶6 | Y＝2.08+1.56X | 80 | 100.5-57.6 | 252 |
| F | 15∶4 | Y＝2.22+1.46X | 76 | 107.8-59.9 | 222 |
| G | 18∶1 | Y＝2.93+1.20X | 106 | 161.2-70.0 | 152 |
3, conclusion
According to the Toxicity Determination result, processing A is 368 μ g/g to the EC50 of Alternaria alternate; Treatments B is 156 μ g/g to the EC50 of Alternaria alternate; The EC50 of 5 proportioning C, D, E, F, G is respectively 159 μ g/g, 242 μ g/g, 80 μ g/g, 76 μ g/g, 106 μ g/g; Co-toxicity coefficient maximum (CTC=252) after hymexazo and network ammonia copper are mixed at 13: 6 Zhong 5 proportionings; Show as synergistic effect, be best proportioning.Suggestion confirms that further by field control effectiveness test 19% dislikes the prevention effect of mould network copper aqua to Alternaria alternate.
Claims (3)
1. network ammonia copper and the anti-Alternaria alternate combination agent of hymexazo comprise network ammonia copper, hymexazo and water, and it is characterized in that: the part by weight of said network ammonia copper and hymexazo is 1: 1～5.
2. network ammonia Tong according to claim 1 Yu the anti-Alternaria alternate combination agent of hymexazo it is characterized in that; Said network ammonia Tong is Yu the part by weight of hymexazo is 1: 2.17.
3. network ammonia Tong according to claim 1 and 2 Yu the anti-Alternaria alternate combination agent of hymexazo it is characterized in that: said network ammonia Tong is Yu the formulation of the anti-Alternaria alternate combination agent of hymexazo is an aqua.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100863147A CN101632387B (en) | 2009-06-10 | 2009-06-10 | Composition preparation of cuaminosulfate and anilazine for resisting tobacco rust |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100863147A CN101632387B (en) | 2009-06-10 | 2009-06-10 | Composition preparation of cuaminosulfate and anilazine for resisting tobacco rust |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101632387A CN101632387A (en) | 2010-01-27 |
| CN101632387B true CN101632387B (en) | 2012-05-09 |
Family
ID=41591855
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100863147A Active CN101632387B (en) | 2009-06-10 | 2009-06-10 | Composition preparation of cuaminosulfate and anilazine for resisting tobacco rust |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101632387B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115119846A (en) * | 2021-08-25 | 2022-09-30 | 汝阳自强生物科技有限公司 | Composite bactericidal composition and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253726A (en) * | 1999-11-24 | 2000-05-24 | 张天良 | Ludi and hymexazol two-component compounded fungicide |
-
2009
- 2009-06-10 CN CN2009100863147A patent/CN101632387B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253726A (en) * | 1999-11-24 | 2000-05-24 | 张天良 | Ludi and hymexazol two-component compounded fungicide |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101632387A (en) | 2010-01-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101637159A (en) | Pesticide composition with synergistic action | |
| CN104872205B (en) | The mixture and its application method prevented and treated for soil-borne disease of tobacco | |
| CN101697740A (en) | Novel traditional Chinese medicine pesticide and production technology thereof | |
| CN101632362B (en) | Uniconazole and pendimethalin bud inhibiting agent | |
| CN102847028A (en) | Traditional Chinese medicine disinfectant and preparation method thereof | |
| CN102633575A (en) | Active biological medicinal fertilizer special for strawberries | |
| CN104886089B (en) | Active polysaccharide application in preventing and treating corn top-rotten disease | |
| CN102669133A (en) | Seed coating agent for preventing ginger wilt | |
| CN104127779A (en) | Antibacterial and anti-inflammatory Chinese herbal medicine compound lotion and preparation method thereof | |
| CN104886163A (en) | Botanical pesticide compound phytolacca acinosa and magnolia officinalis microemulsion and preparation method thereof | |
| CN101632387B (en) | Composition preparation of cuaminosulfate and anilazine for resisting tobacco rust | |
| CN102643140A (en) | Special activity biological pesticide fertilizer for lichees | |
| CN110150330A (en) | A kind of compound botanical source bactericide and preparation method thereof | |
| CN102379316B (en) | Traditional Chinese medicine composition preventing stalk penicillium digitatum and sclerotinia sclerotiorum, preparation method and application thereof | |
| CN101632388B (en) | Composition preparation of cuaminosulfate and propamocarb for resisting tobacco black shank | |
| CN103191356B (en) | Antibacterial spray and preparation method thereof | |
| CN103828830B (en) | Pesticide containing thiamethoxam and chlorothalonil and production process of pesticide | |
| CN104782675A (en) | Novel plant disease antibiotic agent and preparation method thereof | |
| CN103081920B (en) | Sterilizing composition containing penconazole | |
| CN104430490B (en) | A kind of bactericidal composition containing oxalic acid copper diacetonamine | |
| CN105477589A (en) | Pre-surgery disinfecting drug and preparation method | |
| CN104222156A (en) | Sugarcane insecticidal granules containing 20% chlorantraniliprole suspending agent and humic acid | |
| CN104585198B (en) | Containing four chlorantraniliprole Pesticidal combinations | |
| CN108083892A (en) | A kind of organic fertilizer containing Chinese medicine dreg and preparation method thereof | |
| CN101953879B (en) | Microbial ecological agent for quickly and efficiently preventing and treating pig high fever |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171101 Address after: 652108 Huayuan village, South sheep town, Yiliang County, Kunming, Yunnan Patentee after: Kunming Pepsi Biochemical Technology Co., Ltd. Address before: 102488 Liangxiang Fangshan District solid village, Beijing Patentee before: Beijing Dongwang Pesticide Factory |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190125 Address after: 610300 No. 118-1 Tonghui Road, Qingbaijiang Industrial Centralized Development Zone, Chengdu City, Sichuan Province Patentee after: Sichuan Pepsi Dongwang Biotechnology Co., Ltd. Address before: 652108 Garden Village, Nanyang Town, Yiliang County, Kunming City, Yunnan Province Patentee before: Kunming Pepsi Biochemical Technology Co., Ltd. |