CN101629195A - Method for separating virus variation sequence and separated foot-and-mouth disease virus variation sequence thereof - Google Patents

Abstract

The invention discloses a method for separating virus variation sequence and a separated foot-and-mouth disease virus variation sequence thereof. The method of the invention firstly analyzes a reported virus variation zone and then utilizes a random peptide technology to separate and obtain a plurality of polypeptides which can stimulate virus variation; the polypeptides are used for developing vaccines, and development of vaccines can take account of virus variation, which plays a role of precaution before too late. The invention also obtains a foot-and-mouth disease variation sequence, the amino acid sequence thereof is shown as SEQ ID NO:101-200, and corresponding nucleotide sequence is shown as SEQ ID NO:1-100.The invention has simple operating method and can effectively discover a new variation sequence of the virus, which effectively secures national livestock and poultry breeding industry, develops a new way for developing broad-spectrum vaccines and also provides a new technical direction for developing bird flu vaccines, HIV vaccines and the like.

Description

A kind of method of isolated viral series of variation and isolating foot and mouth disease virus series of variation thereof

Technical field

The present invention relates to biological technical field, be specifically related to the virus epidemic prevention, relate in particular to a kind of method and isolating foot and mouth disease virus series of variation thereof of isolated viral series of variation.

Background technology

Foot and mouth disease (foot and mouth disease, FMD) be a kind of acute, hot, hyperinfection sexually transmitted disease artiodactylous, be by foot and mouth disease virus (foot and mouth disease virus, FMDV) infect the transmissible disease of the infecting both domestic animals and human cause, it is livestock contagious disease the most serious in the world today, cloven-hoofed animals such as harm ox, pig, sheep, with propagate rapidly, infection rate is high and celebrated, International Office of Epizootics classifies it first of category-A transmissible disease as.The all popular foot and mouth disease of crossing of overwhelming majority of countries in the world, at present should disease mainly be region in part countries and regions, Asia, Africa, the Middle East and South America popular or sporadicly distribute.

Because the quick variation of virus; aftosa vaccine is difficult to immunized animal is reached effective protection; eliminate the countries and regions of foot and mouth disease and usually can break out foot and mouth disease again; as 1967; the foot and mouth disease that continues 5 months has taken place in Britain; in order to check spreading of disease; have 442,000 bosses poultry to be killed in several thousand popular epidemic-stricken areas, foot and mouth disease was broken out once more in Britain in 1981; foot and mouth disease was at first in Asia and Africa outburst in 2000; then spread to Europe and America, calendar year 2001, foot and mouth disease took place again in Britain, found 2030 of cases altogether; have more than 400 ten thousand livestocks therebetween and butchered, suffer heavy losses.Because the control foot and mouth disease mainly is the measure of butchering of taking at present, therefore brings crushing blow to livestock and poultry breeding industry.

Current; the most economical effective means of control virus disease are exactly vaccine; yet quick variation along with virus; the phenomenon of forfeiture provide protection appears in increasing vaccine; this situation all appears as influenza vaccines, avian influenza vaccine, Hepatitis B virus vaccine etc.; how can be at fast this situation of virus variation, proposing suitable effective solution is a difficult problem of putting at present in face of people.

Summary of the invention

The objective of the invention is at the deficiencies in the prior art, provide a kind of by the virus variation zone is analyzed, and in conjunction with peptide technology at random, the method for isolated viral series of variation.

Another object of the present invention is to provide and adopts aforesaid method to separate the foot and mouth disease virus series of variation that obtains.

Above-mentioned purpose of the present invention is achieved by following scheme:

Fast at virus variation speed; existing vaccine easily loses the phenomenon of provide protection; the invention provides a kind of method of isolated viral series of variation; this method is by analyzing the virus variation zone earlier; utilize peptide technology at random then, separate obtain can the simulated virus variation many polypeptide, these polypeptide are used for the vaccine research and development; vaccine development can play the effect of providing for a rainy day so that can be taken into account the variation of virus.

The method of isolated viral series of variation of the present invention comprises the steps:

(1) analyzes aminoacid sequence viral in the comparison database, according to existing research to this virus antigenicity, find out the encoding sequence in this virus antigen determinant site and the encoding sequence in hypervariable region territory, and the design mixed polypeptide, according to this mixed polypeptide design random primer;

(2) above-mentioned random primer is carried out the asymmetric PCR splicing;

(3) above-mentioned splicing product and yeast secreted expression signal peptide sequence are prepared fusion gene; This fusion gene and Escherichia coli-Saccharomyces cerevisiae shuttle vectors are carried out double digestion respectively, and the Escherichia coli-Saccharomyces cerevisiae shuttle vectors carries out an enzyme again between double enzyme site cuts, and enzyme is cut back calf intestinal alkaline phosphatase (CIAP) processing; Three enzymes of the double digestion product of fusion gene and Escherichia coli-Saccharomyces cerevisiae shuttle vectors are cut product to be connected;

(4) above-mentioned connection product electricity is swashed transformed into escherichia coli, obtain plasmid, after enzyme was cut and handled this plasmid, electricity is sharp once more transformed, and converted product is after screening, and picking the clone check order, and promptly separates obtaining virus variation sequence.

In the above-mentioned steps (1), T cell antigen determinant site, B cell antigen determinant site etc. can be selected in virus antigen determinant site.

In the above-mentioned steps (2), existing splicing to random primer has a variety of methods, as shotgun, restriction enzyme splicing method etc., the inventor finds random primer is adopted the asymmetric PCR splicing by test, can obtain splicing effect preferably, the operation of asymmetric PCR adopts this area routine operation to get final product.

In the above-mentioned steps (3), for splicing product adds the yeast secreted expression signal peptide sequence, can make splicing product well secreting, expressing outside born of the same parents, be easy to purifying, the yeast secreted expression signal sequence can adopt any one those skilled in the art to use always, as the signal peptide sequence of yeast saccharomyces cerevisiae α-mating factor (AMF).

In the above-mentioned steps (3), the Escherichia coli-Saccharomyces cerevisiae shuttle vectors can adopt any one those skilled in the art to use always, as pYES2/CT, pYES6/CT or YEp352 etc.

In the above-mentioned steps (3), the Escherichia coli-Saccharomyces cerevisiae shuttle vectors is carried out three enzymes to be cut, be because routine operation---double digestion often can not be cut thoroughly by enzyme, stay the small content of cyclic dna vector, these do not insert segmental empty carrier can form a large amount of bacterium colonies, thereby influence the screening of positive bacterium colony, and the present invention adopts three enzymes to cut the processing carrier, it is more thorough to make enzyme cut, carrying out a step CIAP after enzyme is cut again handles, make do not insert that the segmental empty carrier of purpose is cut into behind linear DNA and the dephosphorylation can not be by transformed into escherichia coli, thereby can reduce sneaking into of non-recombinant plasmid, guarantee final obtain be positive colony.

In the above-mentioned steps (4), above-mentioned connection product is carried out the intestinal bacteria electricity swash conversion and coated plate, receive bacterium and growth back extraction plasmid, and handle the plasmid that is extracted with restriction enzyme NotI and CIAP, do not insert segmental empty carrier thoroughly to remove, and then the sharp conversion of electricity, converted product is coated the LBGG acillin resistant panel that contains anthocyanidin, picking the clone check order, and promptly separates obtaining virus variation sequence.

The present invention adopts the method for above-mentioned isolated viral series of variation, and the series of variation of foot and mouth disease virus is separated, and its concrete steps are as follows:

(1) the proteic aminoacid sequence of a plurality of O type foot and mouth disease viruses (FMDV) VP1 is analysed and compared in the search database, the mixed polypeptide that design has the E-F-PPS-G structure, wherein the E section is relevant with T cell antigen determinant site, the F section is relevant with B cell antigen determinant site, the G section is G-H ring zone, this G-H ring zone is a neutrality antigen site main in the foot and mouth disease virus, be connected by one two proline(Pro) joint (PPS) between F section and the G section, go up important variable region because the G-H ring is an O type foot and mouth disease virus (FMDV), therefore the G section is designed to the zone of restricted randomization; According to the mixed polypeptide of this E-F-PPS-G structure, the design random primer;

(2) above-mentioned random primer is carried out the asymmetric PCR splicing;

(3) above-mentioned splicing product and yeast secreted expression signal peptide sequence are prepared fusion gene; This fusion gene and Escherichia coli-Saccharomyces cerevisiae shuttle vectors are carried out double digestion respectively, and the Escherichia coli-Saccharomyces cerevisiae shuttle vectors carries out an enzyme again between double enzyme site cuts, and enzyme is cut back calf intestinal alkaline phosphatase (CIAP) processing; Three enzymes of the double digestion product of fusion gene and Escherichia coli-Saccharomyces cerevisiae shuttle vectors are cut product to be connected;

(4) above-mentioned connection product is carried out the efficient electricity of intestinal bacteria and swash conversion and coated plate, receive bacterium and growth back extraction plasmid, and handle the plasmid that is extracted with restriction enzyme NotI and CIAP, make do not insert that the segmental empty carrier of purpose is cut into behind linear DNA and the dephosphorylation can not be by transformed into escherichia coli, thereby can reduce sneaking into of non-recombinant plasmid.And then the sharp conversion of efficient electricity, converted product is coated the LBGG acillin resistant panel that contains anthocyanidin, and picking the clone check order, and promptly separates obtaining virus variation sequence.，

In the above-mentioned steps (1), from Genebank, search the proteic amino-acid sequence of having reported at present of following multiple O type foot and mouth disease virus (FMDV) VP1: AJ294914, AJ303520, AJ294918, AJ303521, AJ296328, AJ004663, AJ303522, AJ303484, AJ303523, AJ303483, AJ318833, AJ318844, AJ318847, AJ303525, AJ303526, AJ318848, AJ131469, AF095884, AF095885, AF026168, AJ296322, AJ294923, AJ318836, AJ294925, AJ294922, AJ294924, AF525458, AJ131468, AJ131470, AJ294917, AJ294913, AJ294915, AJ294916, AJ294919 and AJ294920.According to these amino acid whose analyses and comparison, design mixed polypeptide with E-F-PPS-G structure, wherein the E section is ETQTQRRHHTDVAFVLDRFV, the F section is RHKQPLIAPAKQLL, the G section is designed to part (D/T/V/G/E/Q) (T/A/G/S) (S/R/H/P/A/L) (T/V/A/L) (N/S/T/A) (E/A/T) (E/A/T) two kinds of R (T/A/P/S) LP of R (T/A/P/S) Lp and completely random peptide chain KYXXXXXXXVRGDL (Q/R) VL (A/T) QK (A/T) of (N/K/T) VRGDL (Q/R) VL (A/T) QK (A/T) of peptide chain KY (G/S/A) at random, specific as follows shown in:

A. the part of this mixed polypeptide at random peptide chain be (D/T/V/G/E/Q) (T/A/G/S) (S/R/H/P/A/L) (T/V/A/L) (N/S/T/A) (E/A/T) R (T/A/P/S) LP of (N/K/T) VRGDL (Q/R) VL (A/T) QK (A/T) of ETQTQRRHHTDVAFVLDRFV-RHKQPLIAPAKQLL-PPS-KY (G/S/A), this part at random the nucleotide sequence of peptide chain shown in SEQ ID No:201;

B. the completely random peptide chain of this mixed polypeptide is (E/A/T) R (T/A/P/S) LP of ETQTQRRHHTDVAFVLDRFV-RHKQPLIAPAKQLL-PPS-KYXXXXXXXVRGDL (Q/R) VL (A/T) QK (A/T), and the nucleotide sequence of this completely random peptide chain is shown in SEQ ID NO:202.

In the above-mentioned steps (1), design random primer fmd1 (its nucleotide sequence is shown in SEQ ID No:203) respectively according to sequence SEQ ID No:1 and SEQ ID NO:2, fmd2 (its nucleotide sequence is shown in SEQ ID No:204), fmd3 (its nucleotide sequence is shown in SEQ ID No:205), fmd4 (its nucleotide sequence is shown in SEQ ID No:206) and fmd5 (its nucleotide sequence is shown in SEQ ID No:207), wherein primer fmd4 is mainly at the design of G-H ring, because the G-H ring is hypervariable region, therefore adopt part to design primer fmd4 at random, simultaneously design primer fmd4b (its nucleotide sequence is shown in SEQ ID No:208) according to completely random again, there are 7 codons to adopt the VNN codon among the primer fmd4b, except do not encode die aromatischen Aminosaeuren and halfcystine, other arbitrary amino acid of codified.

In the above-mentioned steps (2), adopt asymmetric PCR that random primer is spliced, the asymmetric PCR operation of adopting those skilled in the art to use always all can realize the present invention.

In the above-mentioned steps (4), adopted twice ultra-high efficiency electricity to swash and handled; Screening can add an amount of anthocyanidin during transformant on used resistant panel, because peptide normally has a bit toxicly at random, and the adding of antioxidant anthocyanidin can weaken toxicity, make the transformant growth better.

In the method for isolated viral series of variation of the present invention, utilizing the XhoI enzyme to cut is not insert the segmental empty carrier of purpose in order to remove major part, have only these empty carriers just to have the XhoI site, in case also can not be transformed large intestine behind the dephosphorylation after being cut into linear DNA, can be reduced sneaking into of non-recombinant plasmid; Utilizing the NotI enzyme to cut is not insert the segmental empty carrier of purpose in order to remove major part, just have only these empty carriers to have the NotI site, in case also can not be transformed large intestine behind the dephosphorylation after being cut into linear DNA, can reduce sneaking into of non-recombinant plasmid.

The present invention adopts above-mentioned separation method, finally obtains 100 foot and mouth disease virus variation sequences, and its aminoacid sequence is shown in SEQ ID NO:101～200, and its corresponding nucleotide sequences is shown in SEQ ID NO:1～100.

Separation method of the present invention is applicable to and separates various new virus variation sequences, these virus variation sequences can be used for the preparation of virus vaccines, because but these virus variation sequence simulated virus are evolved, therefore prepared vaccine can play the effect that takes into account virus variation.

100 foot and mouth disease virus variation sequences that the present invention obtains can be used for the preparation of foot and mouth disease virus vaccine.

Dna encoding sequence of the present invention: wherein, M=A/C, R=A/G, W=A/T, S=G/C, Y=C/T, K=G/T, V=A/G/C, H=A/C/T, D=A/G/T, B=G/C/T, N=A/G/C/T; X represents arbitrary amino acid.

Compared with prior art, the present invention has following beneficial effect:

1. the invention provides a kind of applicable to the method for separating various new virus variation sequences, can be used for the development of vaccine by the virus variation sequence of this method acquisition, because variation is big characteristics of virus, and vaccine is the most effective means of anti-system viral infectious disease, therefore utilize gained virus variation sequence of the present invention to develop vaccine, can take into account the variation of virus, play the effect of providing for a rainy day, Economic development and human health are produced good, huge benefit;

2. separation method of the present invention utilizes at random the peptide technology to carry out the research and development of mixed polypeptide vaccine, simultaneously separation method is optimized improvement, as adopt asymmetric PCR to carry out the random primer splicing, adopt three enzymes to cut with the CIAP processing, adopt efficient electricity to swash conversion etc. to intestinal bacteria-yeast shuttle expression carrier, all make the present invention obtain good separating effect;

3. method of the present invention can be found the series of variation that virus is new simply, efficiently, for the wide spectrum developing vaccines is opened up new way, also the livestock and poultry breeding industry for the whole nation brings sound assurance, also can be for developing accumulation valuable experience such as corresponding avian influenza vaccine, HIV (human immunodeficiency virus) vaaccine.

Embodiment

Below in conjunction with specific embodiment the present invention is done explanation further, but specific embodiment is not done any qualification to the present invention.

Embodiment 1

The separation method of the foot and mouth disease virus series of variation of present embodiment, its concrete steps are as follows:

(1) from Genebank, searches the aminoacid sequence of following a plurality of O type foot and mouth disease virus VP1 Protein G-H ring and these aminoacid sequences are analysed and compared: AJ294914, AJ303520, AJ294918, AJ303521, AJ296328, AJ004663, AJ303522, AJ303484, AJ303523, AJ303483, AJ318833, AJ318844, AJ318847, AJ303525, AJ303526, AJ318848, AJ131469, AF095884, AF095885, AF026168, AJ296322, AJ294923, AJ318836, AJ294925, AJ294922, AJ294924, AF525458, AJ131468, AJ131470, AJ294917, AJ294913, AJ294915, AJ294916, AJ294919 and AJ294920; Design mixed polypeptide with E-F-PPS-G structure according to the concensus sequence of these aminoacid sequences, the aminoacid sequence of this mixed polypeptide is (D/T/V/G/E/Q) (T/A/G/S) (S/R/H/P/A/L) (T/V/A/L) (N/S/T/A) (E/A/T) R (T/A/P/S) Lp of (N/K/T) VRGDL (Q/R) VL (A/T) QK (A/T) of ETQTQRRHHTDVAFVLDRFV-RHKQPLIAPAKQLL-PPS-KY (G/S/A), and its nucleotide sequence is shown in SEQ ID No:201; Design random primer fmd1 (its nucleotide sequence is shown in SEQ ID No:203), fmd2 (its nucleotide sequence is shown in SEQ ID No:204), fmd3 (its nucleotide sequence is shown in SEQ ID No:205), fmd4 (its nucleotide sequence is shown in SEQ ID No:206) and fmd5 (its nucleotide sequence is shown in SEQ ID No:207) according to this mixed polypeptide;

(2) above-mentioned random primer is spliced by asymmetric PCR, its process is as follows:

A. utilize the asymmetric PCR technology, by weight fmd2: fmd3=15: 1 and fmd4: fmd5=1: 15, the Pfu archaeal dna polymerase (commercially available) that adopts high-fidelity carries out primer fmd2 and fmd3 respectively under the situation that the 2-Pyrrolidone (volume percent) that has 2.5% exists, and the anastomosing and splicing of primer fmd4 and fmd5, its PCR reaction conditions is 94 ℃ of pre-sex change 2 minutes, 94 ℃ of sex change 20 seconds, 35 ℃ of annealing 45 seconds, 72 ℃ were extended 35 seconds, carry out 26 circulations, last 70 ℃ were extended 5 minutes;

B. splicing product fmd23 and the fmd45 with above-mentioned PCR reaction mixes with weight ratio at 1: 4, continues to use above-mentioned reaction conditions to increase, and is 30 ℃ except changing annealing temperature, and all the other conditions are constant, thereby obtain the fmd25 splicing product of a long 175bp; With primer fmd1 and the fmd25 mixed in molar ratio to equate, amplify the product fmd15 of long 220bp then, the condition of this reaction is 94 ℃ of pre-sex change 2 minutes, 95 ℃ of sex change 30 seconds, 27 ℃ of annealing 45 seconds, 72 ℃ were extended 30 seconds, carry out 30 circulations, last 70 ℃ were extended 5 minutes;

(3) above-mentioned splicing product is connected again the downstream of the Kex2 signal cleavage site sequence of yeast saccharomyces cerevisiae α-mating factor secretion signal by PCR, its process is as shown in Figure 2: (commercially available with 40ng plasmid pPICZ α A, contain yeast saccharomyces cerevisiae α-mating factor secretory signal sequence) and 50ng fmd15 be template, with P6 (its nucleotide sequence is shown in SEQ ID NO:209) and fmd5 is primer, the chimeric fragment of the fragment of yeast saccharomyces cerevisiae α-mating factor secretion signal of pcr amplification 475bp and combination polypeptide, the PCR reaction conditions is 94 ℃ of pre-sex change 2 minutes, 94 ℃ of sex change 20 seconds, annealed 45 seconds for 37 ℃, 72 ℃ were extended 35 seconds, carry out 30 circulating reactions, last 70 ℃ were extended 5 minutes, and gained PCR product is fusion gene;

Gel reclaims above-mentioned PCR product, uses the EcoRI of 40U and XbaI double digestion 4 hours then, and enzyme was cut 70 ℃ of inactivations of product 15 minutes subsequently, with being melted into 30 μ l deionized waters behind the ethanol sedimentation;

Shuttle plasmid pYES2/CT each EcoRI, XhoI and XbaI enzyme cutting with 40U of yeast saccharomyces cerevisiae are spent the night, and handle three enzymes with 40U CIAP and cut product, carry out enzyme deactivation with after product and handle and use ethanol sedimentation; Because XhoI is between EcoRI and XbaI, utilizing the XhoI enzyme to cut is not insert the segmental empty carrier of purpose in order to remove major part, have only these empty carriers just to have the XhoI site,, can reduce sneaking into of non-recombinant plasmid in case can not be transformed large intestine after being cut into linear DNA;

Get the double digestion product of the above-mentioned fusion gene of 350ng and three enzymes of plasmid pYES2/CT and cut product, carry out ligation at 10: 1 with mol ratio, and connect product with ethanol sedimentation;

(4) the connection product electricity consumption behind the above-mentioned ethanol sedimentation is swashed the efficient DH10B of conversion competent cell (ElectroMax-DH10B, commercially available), be coated on 30 ℃ of cultivations on the LBGG acillin resistant panel that is added with 10mg/L anthocyanidin, washing spreads cultivation behind the transformant down and extracts plasmid, and spend the night with restriction enzyme 20U NotI and 16U CIAP and to handle the plasmid extracted, utilizing the NotI enzyme to cut is in order to remove the remaining segmental empty carrier of not insertion purpose, have only these empty carriers just to have the NotI site, in case also can not be transformed large intestine behind the usefulness CIAP dephosphorylation after being cut into linear DNA, can be reduced sneaking into of non-recombinant plasmid.And then the sharp conversion of electricity (operation is the same), converted product is coated the LBGG acillin resistant panel that contains 10mg/L anthocyanidin, 37 ℃ of cultivations;

With primer P7 (its nucleotide sequence is shown in SEQ ID NO:210) and P8 (its nucleotide sequence is shown in SEQ ID NO:211) pairing the bacterium colony after the above-mentioned cultivation being carried out bacterium colony PCR detects, positive bacterium colony should produce corresponding 730bp band, there is clone's of 80% can detect the fragment of these corresponding sizes, extract the plasmid of single bacterium colony at last, check order with primer T7 (its nucleotide sequence is shown in SEQ ID NO:212), measured clone about 100, the clone who has obtained correct sequence uses reverse primer P9 (its nucleotide sequence is shown in SEQ ID NO:213) to carry out the backward sequencing check again.

Order-checking is to be finished by the ABI3730XL sequenator at auspicious bio tech ltd of Guangzhou scape and China order-checking center, big gene Shenzhen, and most sequence Qv values are greater than 30, and the probability that shows mistake is less than 0.1%.Indivedual base Qv values of indivedual sequences are about 10-20, the probability that shows mistake is about 10%-1%, the foot and mouth disease virus series of variation that present embodiment obtains is that (its aminoacid sequence is shown in SEQID NO:100～110 for Y1～Y10, corresponding nucleotide sequence is shown in SEQ ID NO:1～10), (its aminoacid sequence is shown in SEQ ID NO:123～130 in Y23～30, corresponding nucleotide sequence is shown in SEQ ID NO:23～30), Y32～Y94 (its aminoacid sequence is shown in SEQ ID NO:132～194, and corresponding nucleotide sequence is shown in SEQ ID NO:32～94).

Embodiment 2

The separation method of the foot and mouth disease virus series of variation of present embodiment, in order to reach the segmental purpose of completely randomization, adopt primer fmd4b to replace primer fmd4, all the other preparation processes are with embodiment 1, the foot and mouth disease virus series of variation that present embodiment obtains is that (its aminoacid sequence is shown in SEQ ID NO:111～122 for Y11～Y22, corresponding nucleotide sequence is shown in SEQ ID NO:11～22), Y95～Y100 (its aminoacid sequence is shown in SEQ ID NO:195～200, and corresponding nucleotide sequence is shown in SEQ IDNO:95～100).

Embodiment 3

The separation method of the foot and mouth disease virus series of variation of present embodiment, the acquisition of its first step random primer are with embodiment 1, and its excess-three step is as follows:

(2) (contain yeast saccharomyces cerevisiae α-mating factor secretory signal sequence with plasmid pPICZ α A, commercially available) as template, with P6 (its nucleotide sequence is shown in SEQ ID NO:209) is upstream primer, with fmd1 (its nucleotide sequence is shown in SEQ ID No:203) is downstream primer, adopt the active PfuDNA polysaccharase of high-fidelity (commercially available) to carry out the PCR reaction, its PCR reaction conditions is 94 ℃ of pre-sex change 5 minutes, 94 ℃ of sex change are 30 seconds then, annealed 40 seconds for 46 ℃, 72 ℃ were extended 1 minute, 30 circulating reactions, and last 72 ℃ were extended 10 minutes, obtain amplified production 1, its length is 305bp;

Simultaneously, with primer fmd2 (its nucleotide sequence is shown in SEQ ID No:204), fmd3 (its nucleotide sequence is shown in SEQ ID No:205), fmd4 (its nucleotide sequence is shown in SEQ ID No:206) and fmd5 (its nucleotide sequence is shown in SEQ ID No:207) were according to 2: 1: 1: 2 mol ratio adds in the PCR pipe, adopt Pfu DNAPolymerase to increase under the situation that 2-Pyrrolidone 2.5% exists, its reaction conditions is 95 ℃ of pre-sex change 5 minutes, 95 ℃ of sex change 30 seconds, annealed 2 minutes for 44 ℃, 72 ℃ were extended 2 minutes, carry out 30 circulations, at last, 72 ℃ were extended 10 minutes, and obtained amplified production 2;

Above-mentioned amplified production that obtains 1 and amplified production 2 are mixed as template (volume is 400 μ l), with P6 (its nucleotide sequence is shown in SEQ ID NO:209) is upstream primer, with fmd5 (its nucleotide sequence is shown in SEQ ID No:207) is downstream primer, adopt Pfu DNA Polymerase to carry out the PCR reaction under the situation that 2-Pyrrolidone 2.5% exists, its reaction conditions is as follows: 95 ℃ of pre-sex change 5 minutes, 95 ℃ of sex change 30 seconds, annealed 1 minute 40 seconds for 33 ℃, 72 ℃ were extended 2 minutes, carry out 30 circulations, last, 72 ℃ were extended 10 minutes, obtaining length is the fragment of 457bp, and product is dissolved in 50 μ l ddH after with ethanol sedimentation ₂O.

(3) get above-mentioned through ethanol sedimentation and be dissolved in ddH ₂The amplified production 80ng of O, EcoRI and XbaI cut at 37 ℃ of enzymes and spend the night, and use ethanol inactivation, precipitation again, are dissolved in 10 μ l distilled waters behind the oven drying at low temperature; The yeast saccharomyces cerevisiae shuttle plasmid pYES2/CT of 15 μ g is carried out EcoRI and XbaI double digestion, wherein 6 μ g handled 30 minutes with 10UCIAP, remove 5 ' phosphate anion, prevent that carrier is from connecting, enzyme is cut product phenol/chloroform extracting, and use ethanol sedimentation, be dissolved in 60 μ l distilled waters behind the oven drying at low temperature; Get the amplified production (using the EcoRI/XbaI double digestion) of 200ng pYES2/CT (using the EcoRI/XbaI double digestion) and 150ng, add 3 μ l T4DNA ligase enzyme buffer and 15U T4DNA ligase enzyme, 4 ℃ of connections are spent the night, connect product with ethanol inactivation and precipitation, be dissolved in 13 μ l distilled waters behind the oven drying at low temperature;

(4) above-mentioned connection product is converted into intestinal bacteria XL1-Blue MRF ' bacterial strain (commercially available) by electricity is sharp, is coated with LB/ amicillin resistance flat board, 37 ℃ of overnight incubation; Picking list bacterium colony on the resistant panel changes 5mlLB/ penbritin liquid nutrient medium over to, and 37 ℃ are spent the night, and the 200rpm shaking table is cultivated back upgrading grain; Bacterium colony on the resistant panel is washed with sterilized water, after the enlarged culturing, a large amount of upgrading grains; Gained mixing plasmid is handled with restriction enzyme 20U NotI;

Because between the EcoRI of pYES2/CT plasmid and XbaI site the NotI restriction enzyme site is arranged, this site does not exist on the segmental recombinant plasmid of insertion having.After this uses restriction enzyme NotI cutting,, therefore can reduce sneaking into of non-recombinant plasmid because the linear fragment transformation efficiency is very low.With the electric once more XL1-Blue MRF ' bacterial strain that changes of the plasmid of gained, be coated with LB/ amicillin resistance flat board, cultivated 15 hours for 37 ℃, picking list bacterium colony is as pcr template, with P7 (its nucleotide sequence is shown in SEQ ID NO:210) is upstream primer, with fmd3 (its nucleotide sequence is shown in SEQ ID No:205) is downstream primer, carry out the PCR reaction, its condition is as follows: 95 ℃ of pre-sex change 5 minutes, 95 ℃ of sex change 30 seconds, 53 ℃ of annealing 1 minute, 72 ℃ were extended 1 minute, carry out 41 circulations, at last, 72 ℃ were extended 10 minutes, detected after the fragment of 490bp in the amplified production just with the order-checking of P9 (its nucleotide sequence is shown in SEQ ID NO:213) primer, the aminoacid sequence of having found the ZX sequence is shown in SEQ ID NO:131, and its coding nucleotide sequence is shown in SEQ ID NO:31.

LB prescription used in the foregoing description 1,2,3 and 4 is (500ml): the 5g peptone, and 2.5g yeast extract (commercially available), 5g sodium-chlor is mended to 500ml with distilled water, pH7.0.

LBGG prescription used in the foregoing description 1,2 and 4 is (500ml): the 5g peptone, and 2.5g yeast extract (commercially available), 5g sodium-chlor, 2% glucose, 4% glycerine is mended to 500ml with distilled water, pH7.0.

Used anthocyanidin is last Haikang nine chemical industry company limited products in the foregoing description 1,2 and 4, and purity is 98.75%, and it uses final concentration is 10 mg/litre.

A kind of method of isolated viral series of variation and the sequence table of isolating foot and mouth disease virus series of variation thereof

SEQUENCE?LISTING

＜110〉Zhongshan University, Guangdong Wen's Food Group Co., Ltd.

＜120〉a kind of method of isolated viral series of variation and isolating foot and mouth disease virus series of variation thereof

<130>

<160>213

<170>PatentIn?version?3.5

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acattgccat?ga 192

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gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

cattgtcttt?cgtctaaagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgataga 180

acattgccat?ga 192

<210>4

<211>192

<212>DNA

＜213〉artificial sequence

<400>4

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

ctatctgttt?tggctactgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>5

<211>192

<212>DNA

＜213〉artificial sequence

<400>5

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gttggtccat?ttgatacagt?cagaggtgat?ttgcaagttt?tgactcaaaa?agctgataga 180

acattgccat?ga 192

<210>6

<211>192

<212>DNA

＜213〉artificial sequence

<400>6

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gattctgttg?ttcataatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgcaaga 180

acattgccat?ga 192

<210>7

<211>192

<212>DNA

＜213〉artificial sequence

<400>7

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gattctgctg?tgtctactgt?cagaggtgat?ttgcaagttt?tgactcaaaa?agctgaaaga 180

acattgccat?ga 192

<210>8

<211>192

<212>DNA

＜213〉artificial sequence

<400>8

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gttgctctat?tgcctacagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

ccattgccat?ga 192

<210>9

<211>192

<212>DNA

＜213〉artificial sequence

<400>9

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

catggtcgat?tggatactgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>10

<211>192

<212>DNA

＜213〉artificial sequence

<400>10

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

ctaagtgttg?tgtctactgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgcaaga 180

acattgccat?ga 192

<210>11

<211>192

<212>DNA

＜213〉artificial sequence

<400>11

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacatt 120

gtaagcatta?gtgttgttgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

tcattgccat?ga 192

<210>12

<211>192

<212>DNA

＜213〉artificial sequence

<400>12

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacatg 120

attcttggca?atggacaagt?cagaggtgat?ttgcgagttt?tggctcaaaa?agctgctaga 180

cctttgccat?ga 192

<210>13

<211>192

<212>DNA

＜213〉artificial sequence

<400>13

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacact 120

agagtgcaca?gagtggccgt?cagaggtgat?ttgcgagttt?tggctcaaaa?agctgcaaga 180

actttgccat?ga 192

<210>14

<211>192

<212>DNA

＜213〉artificial sequence

<400>14

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacaat 120

agagttcgaa?atacaaaagt?cagaggtgat?ttgcgagttt?tgactcaaaa?agctgataga 180

actttgccat?ga 192

<210>15

<211>192

<212>DNA

＜213〉artificial sequence

<400>15

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacata 120

catattagta?gtacgattgt?cagaggtgat?ttgcaagttt?tggctcaaaa?agctgctaga 180

actttgccat?ga 192

<210>16

<211>192

<212>DNA

＜213〉artificial sequence

<400>16

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacaat 120

gtgcatcaaa?atcgtctggt?cagaggtgat?ttgcgagttt?tggctcaaaa?aactgctaga 180

gctttgccat?ga 192

<210>17

<211>192

<212>DNA

＜213〉artificial sequence

<400>17

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacatt 120

atcattccga?gtattgccgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgctaga 180

tctttgccat?ga 192

<210>18

<211>192

<212>DNA

＜213〉artificial sequence

<400>18

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacaga 120

catattgccc?tggtagtggt?cagaggtgat?ttgcaagttt?tggctcaaaa?agctgcaaga 180

tcattgccat?ga 192

<210>19

<211>192

<212>DNA

＜213〉artificial sequence

<400>19

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacatg 120

ataaccagta?caaatggtgt?cagaggtgat?ttgcaagttt?tggctcaaaa?agctgctaga 180

gcattgccat?ga 192

<210>20

<211>192

<212>DNA

＜213〉artificial sequence

<400>20

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacatc 120

attataattc?atgtcaccgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgctaga 180

tctttgccat?ga 192

<210>21

<211>192

<212>DNA

＜213〉artificial sequence

<400>21

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgtt 120

cttagtcatg?atcgtgttgt?cagaggtgat?ttgcgagttt?tggctcaaaa?agctgctaga 180

cctttgccat?ga 192

<210>22

<211>192

<212>DNA

＜213〉artificial sequence

<400>22

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgtt 120

atcgatgtca?ttcgcacggt?cagaggtgat?ttgcgagttt?tgactcaaaa?agctgcaaga 180

gctttgccat?ga 192

<210>23

<211>192

<212>DNA

＜213〉artificial sequence

<400>23

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

catactgttg?ttgataatgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>24

<211>192

<212>DNA

＜213〉artificial sequence

<400>24

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

catactcgaa?ttgctaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?agctgaaaga 180

cctttgccat?ga 192

<210>25

<211>192

<212>DNA

＜213〉artificial sequence

<400>25

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

gtttgtcgtt?cggctacagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

gcattgccat?ga 192

<210>26

<211>192

<212>DNA

＜213〉artificial sequence

<400>26

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

gttactgttt?cggctactgt?cagaggtgat?ttgcgagttt?tggctcaaaa?aactgaaaga 180

ccattgccat?ga 192

<210>27

<211>192

<212>DNA

＜213〉artificial sequence

<400>27

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

ctatgtcgag?ctgctaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgataga 180

acattgccat?ga 192

<210>28

<211>192

<212>DNA

＜213〉artificial sequence

<400>28

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

catgctcttg?ttgatacagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

tcattgccat?ga 192

<210>29

<211>192

<212>DNA

＜213〉artificial sequence

<400>29

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

cttagtggag?ctgctactgt?cagaggtgat?ttgcaagttt?tggctcaaaa?agctgcaaga 180

acattgccat?ga 192

<210>30

<211>192

<212>DNA

＜213〉artificial sequence

<400>30

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaccaattgt?tgccaccatc?taagtacggt 120

ctaactgttg?ctactaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>31

<211>192

<212>DNA

＜213〉artificial sequence

<400>31

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

gatggtctat?cgtctacagt?cagaggtgat?ttgcgagttt?tggctcaaaa?aactgataga 180

ccattgccat?ga 192

<210>32

<211>192

<212>DNA

＜213〉artificial sequence

<400>32

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaacatc?taagtacggt 120

catgctcgat?cgtctaaagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

cctttgccat?ga 192

<210>33

<211>192

<212>DNA

＜213〉artificial sequence

<400>33

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

catactgctt?ttgctaaagt?cagaggtgat?ttgcgagttt?tgactcaaaa?agctgaaaga 180

ccattgccat?ga 192

<210>34

<211>192

<212>DNA

＜213〉artificial sequence

<400>34

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gttggtcgag?cggctaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?agctgctaga 180

gcattgccat?ga 192

<210>35

<211>192

<212>DNA

＜213〉artificial sequence

<400>35

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

cttagtcttg?ttgataatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

actttgccat?ga 192

<210>36

<211>192

<212>DNA

＜213〉artificial sequence

<400>36

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

cttgctgtat?ttgataatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgcaaga 180

tcattgccat?ga 192

<210>37

<211>192

<212>DNA

＜213〉artificial sequence

<400>37

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

ctatgtcctt?cggctacagt?cagaggtgat?ttgcgagttt?tggctcaaaa?aactgataga 180

ccattgccat?ga 192

<210>38

<211>192

<212>DNA

＜213〉artificial sequence

<400>38

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

gttagtcgat?ttgctactgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgcaaga 180

acattgccat?ga 192

<210>39

<211>192

<212>DNA

＜213〉artificial sequence

<400>39

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gttagtgcag?cggctacagt?cagaggtgat?ttgcaagttt?tggctcaaaa?agctgcaaga 180

acattgccat?ga 192

<210>40

<211>192

<212>DNA

＜213〉artificial sequence

<400>40

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

cttagtgctg?tgactactgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgataga 180

acattgccat?ga 192

<210>41

<211>192

<212>DNA

＜213〉artificial sequence

<400>41

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

ctatgtgcaa?ctcctaaagt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>42

<211>192

<212>DNA

＜213〉artificial sequence

<400>42

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gatactcgtt?tgtctaaagt?cagaggtgat?ttgcgagttt?tgactcaaaa?agctgcaaga 180

ccattgccat?ga 192

<210>43

<211>192

<212>DNA

＜213〉artificial sequence

<400>43

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

catactgtag?ttcctactgt?cagaggtgat?ttgcaagttt?tggctcaaaa?agctgcaaga 180

acattgccat?ga 192

<210>44

<211>192

<212>DNA

＜213〉artificial sequence

<400>44

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

cattgtggtt?tgcataatgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>45

<211>192

<212>DNA

＜213〉artificial sequence

<400>45

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

ctttctctat?ttcatacagt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>46

<211>192

<212>DNA

＜213〉artificial sequence

<400>46

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

gttactctaa?tttctaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>47

<211>192

<212>DNA

＜213〉artificial sequence

<400>47

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

catgctcttg?ctgctacagt?cagaggtgat?ttgcaagttt?tggctcaaaa?agctgaaaga 180

acattgccat?ga 192

<210>48

<211>192

<212>DNA

＜213〉artificial sequence

<400>48

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

ctagctgctg?tggctacagt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgataga 180

tcattgccat?ga 192

<210>49

<211>192

<212>DNA

＜213〉artificial sequence

<400>49

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

catagtgctg?tgcataatgt?cagaggtgat?ttgcgagttt?tgactcaaaa?agctgctaga 180

actttgccat?ga 192

<210>50

<211>192

<212>DNA

＜213〉artificial sequence

<400>50

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gtttctcttg?tgtatactgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgctaga 180

acattgccat?ga 192

<210>51

<211>192

<212>DNA

＜213〉artificial sequence

<400>51

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

ctaggtcgat?tggataatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?agctgcaaga 180

tcattgccat?ga 192

<210>52

<211>192

<212>DNA

＜213〉artificial sequence

<400>52

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

gtttgtgtta?tgtatactgt?cagaggtgat?ttgcaagttt?tggctcaaaa?agctgaaaga 180

acattgccat?ga 192

<210>53

<211>192

<212>DNA

＜213〉artificial sequence

<400>53

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gttggtcttg?ttactaaagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgcaaga 180

gctttgccat?ga 192

<210>54

<211>192

<212>DNA

＜213〉artificial sequence

<400>54

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gtatgtcctg?ttgctaaagt?cagaggtgat?ttgcgagttt?tggctcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>55

<211>192

<212>DNA

＜213〉artificial sequence

<400>55

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

catagtggtt?tggctacagt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

cctttgccat?ga 192

<210>56

<211>192

<212>DNA

＜213〉artificial sequence

<400>56

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

gaagctgcat?cgcatacagt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgcaaga 180

ccattgccat?ga 192

<210>57

<211>192

<212>DNA

＜213〉artificial sequence

<400>57

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgta 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

ctttatggat?tgtctactgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgataga 180

actttgccat?ga 192

<210>58

<211>192

<212>DNA

＜213〉artificial sequence

<400>58

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgcccccatc?taagtactgt 120

cttggtcttt?ctgctactgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgataga 180

gcattgccat?ga 192

<210>59

<211>192

<212>DNA

＜213〉artificial sequence

<400>59

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gaatgtcctt?ctactactgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>60

<211>192

<212>DNA

＜213〉artificial sequence

<400>60

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

catgctcgag?tggatacagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

gcattgccat?ga 192

<210>61

<211>192

<212>DNA

＜213〉artificial sequence

<400>61

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

cattgtgcag?ttcgtacagt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>62

<211>192

<212>DNA

＜213〉artificial sequence

<400>62

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

cttaatctta?tgtctaatgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>63

<211>192

<212>DNA

＜213〉artificial sequence

<400>63

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gatagtgcta?tggataaagt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>64

<211>192

<212>DNA

＜213〉artificial sequence

<400>64

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

ctaggtggtt?tgcctaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgctaga 180

acattgccat?ga 192

<210>65

<211>192

<212>DNA

＜213〉artificial sequence

<400>65

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gtatgtcctt?tggctaatgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgaaaga 180

ccattgccat?ga 192

<210>66

<211>192

<212>DNA

＜213〉artificial sequence

<400>66

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

ctttctctag?ctcatactgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgcaaga 180

acattgccat?ga 192

<210>67

<211>192

<212>DNA

＜213〉artificial sequence

<400>67

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gatactcctt?cttctactgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgataga 180

actttgccat?ga 192

<210>68

<211>192

<212>DNA

＜213〉artificial sequence

<400>68

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gttactcctt?ttcatacagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgataga 180

acattgccat?ga 192

<210>69

<211>192

<212>DNA

＜213〉artificial sequence

<400>69

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gaaggtgcat?ttgctaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?agctgaaaga 180

cctttgccat?ga 192

<210>70

<211>192

<212>DNA

＜213〉artificial sequence

<400>70

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

catactgcat?tttctacagt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgcaaga 180

gcattgccat?ga 192

<210>71

<211>192

<212>DNA

＜213〉artificial sequence

<400>71

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gtaactcttg?tggatacagt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

gcattgccat?ga 192

<210>72

<211>192

<212>DNA

＜213〉artificial sequence

<400>72

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gaagatgtag?ttgatactgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgcaaga 180

actttgccat?ga 192

<210>73

<211>192

<212>DNA

＜213〉artificial sequence

<400>73

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccacaatc?taagtacgct 120

gattgtcgtt?tgactaaagt?cagaggtgat?ttgcaagttt?tagctcaaaa?aactgcaaga 180

ccattgccat?ga 192

<210>74

<211>192

<212>DNA

＜213〉artificial sequence

<400>74

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

ctaggtgcag?tgtctactgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgcaaga 180

tcattgccat?ga 192

<210>75

<211>192

<212>DNA

＜213〉artificial sequence

<400>75

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

ctatgtggtg?tggatacagt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgctaga 180

acattgccat?ga 192

<210>76

<211>192

<212>DNA

＜213〉artificial sequence

<400>76

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

ctttgtgcta?ttaataaagt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgcaaga 180

acattgccat?ga 192

<210>77

<211>192

<212>DNA

＜213〉artificial sequence

<400>77

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

cattctggag?ctcctactgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

ccattgccat?ga 192

<210>78

<211>192

<212>DNA

＜213〉artificial sequence

<400>78

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gatactgctt?tgaataatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

ccattgccat?ga 192

<210>79

<211>192

<212>DNA

＜213〉artificial sequence

<400>79

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

ctttctacag?ctgatactgt?cagaggtgat?ttgcaagttt?tggctcaaaa?aactgaaaga 180

acattgtcat?ga 192

<210>80

<211>192

<212>DNA

＜213〉artificial sequence

<400>80

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gtttgtcgta?tttctactgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgataga 180

acattgccat?ga 192

<210>81

<211>192

<212>DNA

＜213〉artificial sequence

<400>81

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

catgctgtaa?cgtctactgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgcaaga 180

acattgccat?ga 192

<210>82

<211>192

<212>DNA

＜213〉artificial sequence

<400>82

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

gttgctcttg?ctcatacagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>83

<211>192

<212>DNA

＜213〉artificial sequence

<400>83

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

cttgctgctt?tgtctaaagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgcaaga 180

gcattgccat?ga 192

<210>84

<211>192

<212>DNA

＜213〉artificial sequence

<400>84

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

ctaactgctt?ttgctaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgataga 180

actttgccat?ga 192

<210>85

<211>192

<212>DNA

＜213〉artificial sequence

<400>85

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?atccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

gaagctgctt?ttcctaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>86

<211>192

<212>DNA

＜213〉artificial sequence

<400>86

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gttagtattg?tgcataatgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgaaaga 180

acattgccat?ga 192

<210>87

<211>192

<212>DNA

＜213〉artificial sequence

<400>87

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gttgctcttt?tggctaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgataga 180

acattgccat?ga 192

<210>88

<211>192

<212>DNA

＜213〉artificial sequence

<400>88

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

gtaagtcttt?tgtatactgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgcaaga 180

cctttgccat?ga 192

<210>89

<211>192

<212>DNA

＜213〉artificial sequence

<400>89

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacggt 120

catggtccaa?ttgctaatgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgataga 180

acattgccat?ga 192

<210>90

<211>192

<212>DNA

＜213〉artificial sequence

<400>90

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gtaactcctg?cgactaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

actttgccat?ga 192

<210>91

<211>192

<212>DNA

＜213〉artificial sequence

<400>91

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

gttagtcctt?ttaatacagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgaaaga 180

ccattgccat?ga 192

<210>92

<211>192

<212>DNA

＜213〉artificial sequence

<400>92

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgga 120

cattgtgctg?cgaataatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgataga 180

acattgccat?ga 192

<210>93

<211>192

<212>DNA

＜213〉artificial sequence

<400>93

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactct 120

gtttctcttg?cttctactgt?cagaggtgat?ttgcgagttt?tgactcaaaa?aactgaaaga 180

gcattgccat?ga 192

<210>94

<211>192

<212>DNA

＜213〉artificial sequence

<400>94

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtactgt 120

catagtgtag?tgtctacagt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgataga 180

gcattgccat?ga 192

<210>95

<211>192

<212>DNA

＜213〉artificial sequence

<400>95

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacatg 120

agtataatcg?tgaacgcggt?cagaggtgat?ttgcaagttt?tgactcaaaa?aactgataga 180

ccattgccat?ga 192

<210>96

<211>192

<212>DNA

＜213〉artificial sequence

<400>96

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

aaacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgat 120

gtaatcacgc?tgcaggcggt?cagaggtgat?ttgcgagttt?tggctcaaaa?agctgctaga 180

ccattgccat?ga 192

<210>97

<211>192

<212>DNA

＜213〉artificial sequence

<400>97

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgct 120

cattgtgctg?cggatacagt?cagaggtgat?ttgcaagttt?tggctcaaaa?agctgcaaga 180

acattgccat?ga 192

<210>98

<211>192

<212>DNA

＜213〉artificial sequence

<400>98

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacata 120

gatgctcaca?gtgttgatgt?cagaggtgat?ttgcgagttt?tggctcaaaa?aactgctaga 180

tctttgccat?ga 192

<210>99

<211>192

<212>DNA

＜213〉artificial sequence

<400>99

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacatg 120

attattagta?atcttggagt?cagaggtgat?ttgcgagttt?tggctcaaaa?agctgcaaga 180

gcattgccat?ga 192

<210>100

<211>192

<212>DNA

＜213〉artificial sequence

<400>100

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacgtt 120

attattatgc?tggctaatgt?cagaggtgat?ttgcaagttt?tgactcaaaa?agctgctaga 180

cctttgccat?ga 192

<210>101

<211>63

<212>PRT

＜213〉artificial sequence

<400>101

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Leu?Cys?Ala?Phe?Asp?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>102

<211>63

<212>PRT

＜213〉artificial sequence

<400>102

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Val?Ser?Gly?Thr?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>103

<211>63

<212>PRT

＜213〉artificial sequence

<400>103

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?His?Cys?Leu?Ser?Ser?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>104

<211>63

<212>PRT

＜213〉artificial sequence

<400>104

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Leu?Ser?Val?Leu?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>105

<211>63

<212>PRT

＜213〉artificial sequence

<400>105

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Val?Gly?Pro?Phe?Asp?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Ala?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>106

<211>63

<212>PRT

＜213〉artificial sequence

<400>106

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Asp?Ser?Val?Val?His?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>107

<211>63

<212>PRT

＜213〉artificial sequence

<400>107

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Asp?Ser?Ala?Val?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Ala?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>108

<211>63

<212>PRT

＜213〉artificial sequence

<400>108

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Val?Ala?Leu?Leu?Pro?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>109

<211>63

<212>PRT

＜213〉artificial sequence

<400>109

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?His?Gly?Arg?Leu?Asp?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>110

<211>63

<212>PRT

＜213〉artificial sequence

<400>110

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Leu?Ser?Val?Val?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>111

<211>63

<212>PRT

＜213〉artificial sequence

<400>111

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ile?Val?Ser?Ile?Ser?Val?Val?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Ser?Leu?Pro

50 55 60

<210>112

<211>63

<212>PRT

＜213〉artificial sequence

<400>112

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?Hi?s?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Met?Ile?Leu?Gly?Asn?Gly?Gln?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Pro?Leu?Pro

50 55 60

<210>113

<211>63

<212>PRT

＜213〉artificial sequence

<400>113

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Thr?Arg?Val?His?Arg?Val?Ala?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>114

<211>63

<212>PRT

＜213〉artificial sequence

<400>114

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Asn?Arg?Val?Arg?Asn?Thr?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Ala?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>115

<211>63

<212>PRT

＜213〉artificial sequence

<400>115

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ile?His?Ile?Ser?Ser?Thr?Ile?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>116

<211>63

<212>PRT

＜213〉artificial sequence

<400>116

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Asn?Val?His?Gln?Asn?Arg?Leu?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Thr?Ala?Arg?Ala?Leu?Pro

50 55 60

<210>117

<211>63

<212>PRT

＜213〉artificial sequence

<400>117

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ile?Ile?Ile?Pro?Ser?Ile?Ala?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Ala?Arg?Ser?Leu?Pro

50 55 60

<210>118

<211>63

<212>PRT

＜213〉artificial sequence

<400>118

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Arg?His?Ile?Ala?Leu?Val?Val?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Ser?Leu?Pro

50 55 60

<210>119

<211>63

<212>PRT

＜213〉artificial sequence

<400>119

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Met?Ile?Thr?Ser?Thr?Asn?Gly?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Ala?Leu?Pro

50 55 60

<210>120

<211>63

<212>PRT

＜213〉artificial sequence

<400>120

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ile?Ile?Ile?Ile?His?Val?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Ser?Leu?Pro

50 55 60

<210>121

<211>63

<212>PRT

＜213〉artificial sequence

<400>121

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Val?Leu?Ser?His?Asp?Arg?Val?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Pro?Leu?Pro

50 55 60

<210>122

<211>63

<212>PRT

＜213〉artificial sequence

<400>122

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Val?Ile?Asp?Val?Ile?Arg?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Ala?Ala?Arg?Ala?Leu?Pro

50 55 60

<210>123

<211>63

<212>PRT

＜213〉artificial sequence

<400>123

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?His?Thr?Val?Val?Asp?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>124

<211>63

<212>PRT

＜213〉artificial sequence

<400>124

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?His?Thr?Arg?Ile?Ala?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Ala?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>125

<211>63

<212>PRT

＜213〉artificial sequence

<400>125

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Val?Cys?Arg?Ser?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Ala?Leu?Pro

50 55 60

<210>126

<211>63

<212>PRT

＜213〉artificial sequence

<400>126

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Val?Thr?Val?Ser?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>127

<211>63

<212>PRT

＜213〉artificial sequence

<400>127

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Leu?Cys?Arg?Ala?Ala?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>128

<211>63

<212>PRT

＜213〉artificial sequence

<400>128

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?His?Ala?Leu?Val?Asp?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Ser?Leu?Pro

50 55 60

<210>129

<211>63

<212>PRT

＜213〉artificial sequence

<400>129

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Leu?Ser?GlyAla?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>130

<211>63

<212>PRT

＜213〉artificial sequence

<400>130

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Asn?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Leu?Thr?Val?Ala?Thr?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>131

<211>63

<212>PRT

＜213〉artificial sequence

<400>131

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Asp?Gly?Leu?Ser?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Thr?Asp?Arg?Pro?Leu?Pro

50 55 60

<210>132

<211>63

<212>PRT

＜213〉artificial sequence

<400>132

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Thr?Ser?Lys?Tyr?Gly?His?Ala?Arg?Ser?Ser?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>133

<211>63

<212>PRT

＜213〉artificial sequence

<400>133

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?His?Thr?Ala?Phe?Ala?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Ala?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>134

<211>63

<212>PRT

＜213〉artificial sequence

<400>134

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Val?Gly?Arg?Ala?Ala?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Ala?Ala?Arg?Ala?Leu?Pro

50 55 60

<210>135

<211>63

<212>PRT

＜213〉artificial sequence

<400>135

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Leu?Ser?Leu?Val?Asp?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>136

<211>63

<212>PRT

＜213〉artificial sequence

<400>136

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Leu?Ala?Val?Phe?Asp?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Ser?Leu?Pro

50 55 60

<210>137

<211>63

<212>PRT

＜213〉artificial sequence

<400>137

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Leu?Cys?Pro?Ser?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Thr?Asp?Arg?Pro?Leu?Pro

50 55 60

<210>138

<211>63

<212>PRT

＜213〉artificial sequence

<400>138

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Val?Ser?Arg?Phe?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>139

<211>63

<212>PRT

＜213〉artificial sequence

<400>139

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Val?Ser?Ala?Ala?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>140

<211>63

<212>PRT

＜213〉artificial sequence

<400>140

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Leu?Ser?Ala?Val?Thr?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>141

<211>63

<212>PRT

＜213〉artificial sequence

<400>141

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Leu?Cys?Ala?Thr?Pro?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>142

<211>63

<212>PRT

＜213〉artificial sequence

<400>142

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Asp?Thr?Arg?Leu?Ser?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Ala?Ala?Arg?Pro?Leu?Pro

50 55 60

<210>143

<211>63

<212>PRT

＜213〉artificial sequence

<400>143

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?His?Thr?Val?Val?Pro?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>144

<211>63

<212>PRT

＜213〉artificial sequence

<400>144

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?His?Cys?Gly?Leu?His?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>145

<211>63

<212>PRT

＜213〉artificial sequence

<400>145

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Leu?Ser?Leu?Phe?His?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>146

<211>63

<212>PRT

＜213〉artificial sequence

<400>146

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Val?Thr?Leu?Ile?Ser?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>147

<211>63

<212>PRT

＜213〉artificial sequence

<400>147

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?His?Ala?Leu?Ala?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Ala?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>148

<211>63

<212>PRT

＜213〉artificial sequence

<400>148

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Leu?Ala?Ala?Val?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Ser?Leu?Pro

50 55 60

<210>149

<211>63

<212>PRT

＜213〉artificial sequence

<400>149

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?His?Ser?Ala?Val?His?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Ala?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>150

<211>63

<212>PRT

＜213〉artificial sequence

<400>150

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Val?Ser?Leu?Val?Tyr?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>151

<211>63

<212>PRT

＜213〉artificial sequence

<400>151

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Leu?Gly?Arg?Leu?Asp?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Ala?Ala?Arg?Ser?Leu?Pro

50 55 60

<210>152

<211>63

<212>PRT

＜213〉artificial sequence

<400>152

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Val?Cys?Val?Met?Tyr?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Ala?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>153

<211>63

<212>PRT

＜213〉artificial sequence

<400>153

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Val?Gly?Leu?Val?Thr?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Ala?Leu?Pro

50 55 60

<210>154

<211>63

<212>PRT

＜213〉artificial sequence

<400>154

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Val?Cys?Pro?Val?Ala?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>155

<211>63

<212>PRT

＜213〉artificial sequence

<400>155

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?His?Ser?Gly?Leu?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>156

<211>63

<212>PRT

＜213〉artificial sequence

<400>156

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Glu?Ala?Ala?Ser?His?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?ThrAla?Arg?Pro?Leu?Pro

50 55 60

<210>157

<211>63

<212>PRT

＜213〉artificial sequence

<400>157

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Leu?Tyr?Gly?Leu?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>158

<211>63

<212>PRT

＜213〉artificial sequence

<400>158

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Leu?Gly?Leu?Ser?Ala?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Asp?Arg?Ala?Leu?Pro

50 55 60

<210>159

<211>63

<212>PRT

＜213〉artificial sequence

<400>159

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Glu?Cys?Pro?Ser?Thr?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>160

<211>63

<212>PRT

＜213〉artificial sequence

<400>160

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?His?Ala?Arg?Val?Asp?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Ala?Leu?Pro

50 55 60

<210>161

<211>63

<212>PRT

＜213〉artificial sequence

<400>161

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?His?Cys?Ala?Val?Arg?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>162

<211>63

<212>PRT

＜213〉artificial sequence

<400>162

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Leu?Asn?Leu?Met?Ser?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>163

<211>63

<212>PRT

＜213〉artificial sequence

<400>163

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Asp?Ser?Ala?Met?Asp?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>164

<211>63

<212>PRT

＜213〉artificial sequence

<400>164

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Leu?Gly?Gly?Leu?Pro?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>165

<211>63

<212>PRT

＜213〉artificial sequence

<400>165

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Val?Cys?Pro?Leu?Ala?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>166

<211>63

<212>PRT

＜213〉artificial sequence

<400>166

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Leu?Ser?Leu?Ala?His?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>167

<211>63

<212>PRT

＜213〉artificial sequence

<400>167

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Asp?Thr?Pro?Ser?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>168

<211>63

<212>PRT

＜213〉artificial sequence

<400>168

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Val?Thr?Pro?Phe?His?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>169

<211>63

<212>PRT

＜213〉artificial sequence

<400>169

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Glu?Gly?Ala?Phe?Ala?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Ala?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>170

<212>PRT

＜213〉artificial sequence

<400>170

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?His?Thr?Ala?Phe?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Ala?Leu?Pro

50 55 60

<210>171

<212>PRT

＜213〉artificial sequence

<400>171

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Val?Thr?Leu?Val?Asp?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Ala?Leu?Pro

50 55 60

<210>172

<211>63

<212>PRT

＜213〉artificial sequence

<400>172

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Glu?Asp?Val?Val?Asp?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>173

<211>63

<212>PRT

＜213〉artificial sequence

<400>173

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Gln?Ser?Lys?Tyr?Ala?Asp?Cys?Arg?Leu?Thr?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Ala?Arg?Pro?Leu?Pro

50 55 60

<210>174

<211>63

<212>PRT

＜213〉artificial sequence

<400>174

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Leu?Gly?Ala?Val?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Ala?Arg?Ser?Leu?Pro

50 55 60

<210>175

<211>63

<212>PRT

＜213〉artificial sequence

<400>175

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Leu?Cys?Gly?Val?Asp?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>176

<211>63

<212>PRT

＜213〉artificial sequence

<400>176

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Leu?Cys?Ala?Ile?Asn?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>177

<211>63

<212>PRT

＜213〉artificial sequence

<400>177

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?His?Ser?Gly?Ala?Pro?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>178

<211>63

<212>PRT

＜213〉artificial sequence

<400>178

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Asp?Thr?Ala?Leu?Asn?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>179

<211>63

<212>PRT

＜213〉artificial sequence

<400>179

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Leu?Ser?Thr?Ala?Asp?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Ser

50 55 60

<210>180

<211>63

<212>PRT

＜213〉artificial sequence

<400>180

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Val?Cys?Arg?Ile?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>181

<211>63

<212>PRT

＜213〉artificial sequence

<400>181

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?His?Ala?Val?Thr?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>182

<211>63

<212>PRT

＜213〉artificial sequence

<400>182

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Val?Ala?Leu?Ala?His?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>183

<211>63

<212>PRT

＜213〉artificial sequence

<400>183

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Leu?Ala?Ala?Leu?Ser?Lys?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Ala?Leu?Pro

50 55 60

<210>184

<211>63

<212>PRT

＜213〉artificial sequence

<400>184

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Leu?Thr?Ala?Phe?Ala?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>185

<211>63

<212>PRT

＜213〉artificial sequence

<400>185

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?His?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?Glu?Ala?Ala?Phe?Pro?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>186

<211>63

<212>PRT

＜213〉artificial sequence

<400>186

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Val?Ser?Ile?Val?His?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>187

<211>63

<212>PRT

＜213〉artificial sequence

<400>187

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Val?Ala?Leu?Leu?Ala?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>188

<211>63

<212>PRT

＜213〉artificial sequence

<400>188

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?Val?Ser?Leu?Leu?Tyr?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Ala?Arg?Pro?Leu?Pro

50 55 60

<210>189

<211>63

<212>PRT

＜213〉artificial sequence

<400>189

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?His?Gly?Pro?Ile?Ala?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>190

<211>63

<212>PRT

＜213〉artificial sequence

<400>190

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Val?Thr?Pro?Ala?Thr?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Thr?Leu?Pro

50 55 60

<210>191

<211>63

<212>PRT

＜213〉artificial sequence

<400>191

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?Val?Ser?Pro?Phe?Asn?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Pro?Leu?Pro

50 55 60

<210>192

<211>63

<212>PRT

＜213〉artificial sequence

<400>192

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Gly?His?Cys?Ala?Ala?Asn?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Thr?Leu?Pro

50 55 60

<210>193

<211>63

<212>PRT

＜213〉artificial sequence

<400>193

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ser?Val?Ser?Leu?Ala?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Thr?Gln?Lys?Thr?Glu?Arg?Ala?Leu?Pro

50 55 60

<210>194

<211>63

<212>PRT

＜213〉artificial sequence

<400>194

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Cys?His?Ser?Val?Val?Ser?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Ala?Leu?Pro

50 55 60

<210>195

<211>63

<212>PRT

＜213〉artificial sequence

<400>195

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Met?Ser?Ile?Ile?Val?Asn?Ala?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Thr?Asp?Arg?Pro?Leu?Pro

50 55 60

<210>196

<211>63

<212>PRT

＜213〉artificial sequence

<400>196

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Lys?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Asp?Val?Ile?Thr?Leu?Gln?Ala?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Pro?Leu?Pro

50 55 60

<210>197

<211>63

<212>PRT

＜213〉artificial sequence

<400>197

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ala?His?Cys?Ala?Ala?Asp?Thr?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Thr?Leu?Pro

50 55 60

<210>198

<211>63

<212>PRT

＜213〉artificial sequence

<400>198

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Ile?Asp?Ala?His?Ser?Val?Asp?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Thr?Ala?Arg?Ser?Leu?Pro

50 55 60

<210>199

<211>63

<212>PRT

＜213〉artificial sequence

<400>199

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Met?Ile?Ile?Ser?Asn?Leu?Gly?Val?Arg

35 40 45

Gly?Asp?Leu?Arg?Val?Leu?Ala?Gln?Lys?Ala?Ala?Arg?Ala?Leu?Pro

50 55 60

<210>200

<211>63

<212>PRT

＜213〉artificial sequence

<400>200

Glu?Thr?Gln?Thr?Gln?Arg?Arg?His?His?Thr?Asp?Val?Ala?Phe?Val?Leu

1 5 10 15

Asp?Arg?Phe?Val?Arg?His?Lys?Gln?Pro?Leu?Ile?Ala?Pro?Ala?Lys?Gln

20 25 30

Leu?Leu?Pro?Pro?Ser?Lys?Tyr?Val?Ile?Ile?Met?Leu?Ala?Asn?Val?Arg

35 40 45

Gly?Asp?Leu?Gln?Val?Leu?Thr?Gln?Lys?Ala?Ala?Arg?Pro?Leu?Pro

50 55 60

<210>201

<211>189

<212>DNA

＜213〉artificial sequence

<400>201

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtackst 120

swwdstvnwd?yknmtamwgt?cagaggtgat?ttgcragttt?tgrctcaaaa?arctgmwaga 180

ncwttgcca 189

<210>202

<211>189

<212>DNA

＜213〉artificial sequence

<400>202

gaaactcaaa?ctcaaagaag?acatcatact?gatgtcgctt?tcgttttgga?tagatttgtt 60

agacataaac?agccattgat?tgctccagct?aaacaattgt?tgccaccatc?taagtacvnn 120

vnnvnnvnnv?nnvnnvnngt?cagaggtgat?ttgcragttt?tgrctcaaaa?arctgmwaga 180

ncwttgcca 189

<210>203

<211>59

<212>DNA

＜213〉artificial sequence

<400>203

tctctcgaga?aaagagaaac?tcaaactcaa?agaagacatc?atactgatgt?cgctttcgt 59

<210>204

<211>59

<212>DNA

＜213〉artificial sequence

<400>204

catactgatg?tcgctttcgt?tttggataga?tttgttagac?ataaacagcc?attgattgc 59

<210>205

<211>56

<212>DNA

＜213〉artificial sequence

<400>205

gtacttagat?ggtggcaaca?attgtttagc?tggagcaatc?aatggctgtttatgtc 56

<210>206

<211>56

<212>DNA

＜213〉artificial sequence

<400>206

tgttgccacc?atctaagtac?kstswwdstv?nwdyknmtam?wgtcagaggt?gatttg 56

<210>207

<211>63

<212>DNA

＜213〉artificial sequence

<400>207

cctctagatc?atggcaawgn?tctwkcagyt?ttttgagyca?aaactygcaa?atcacctctt 60

gac 63

<210>208

<211>56

<212>DNA

＜213〉artificial sequence

<400>208

tgttgccacc?atctaagtac?vnnvnnvnnv?nnvnnvnnvn?ngtcagaggt?gatttg 56

<210>209

<211>28

<212>DNA

＜213〉artificial sequence

<400>209

gcgaattcaa?aaatgagatt?tccttcaa 28

<210>210

<211>40

<212>DNA

＜213〉artificial sequence

<400>210

cctctatact?ttaacgtcaa?ggagaaaaaa?ccccggatcg 40

<210>211

<211>40

<212>DNA

＜213〉artificial sequence

<400>211

ggcgtgaatg?taagcgtgac?ataactaatt?acatgatgcg 40

<210>212

<211>20

<212>DNA

＜213〉artificial sequence

<400>212

taatacgact?cactataggg 20

<210>213

<211>22

<212>DNA

＜213〉artificial sequence

<400>213

taggatcagc?gggtttaaac?tc 22

Claims (9)

1, a kind of method of isolated viral series of variation is characterized in that this method comprises the steps:

(1) analyze in the comparison database should virus aminoacid sequence, find out the encoding sequence in this virus antigen determinant site and the encoding sequence in hypervariable region territory, design mixed polypeptide, and design the random primer of this mixed polypeptide;

(2) above-mentioned random primer is carried out the asymmetric PCR splicing;

(3) above-mentioned splicing product and yeast secreted expression signal peptide sequence are built into fusion gene; This fusion gene and Escherichia coli-Saccharomyces cerevisiae shuttle vectors are carried out double digestion respectively, and the Escherichia coli-Saccharomyces cerevisiae shuttle vectors again between double enzyme site more to carry out an enzyme and cuts, and enzyme is cut the back and handled with calf intestinal alkaline phosphatase; Three enzymes of the double digestion product of fusion gene and Escherichia coli-Saccharomyces cerevisiae shuttle vectors are cut product to be connected;

(4) above-mentioned connection product electricity is swashed transformed into escherichia coli, obtain plasmid, after enzyme was cut and handled this plasmid, electricity is sharp once more transformed, and converted product is after screening, and picking the clone check order, and promptly separates obtaining virus variation sequence.

2,, it is characterized in that described virus is foot and mouth disease virus according to the described separation method of claim 1.

3, according to the described separation method of claim 2, it is characterized in that in the step (1), described random primer is a nucleotide sequence shown in SEQ ID No:203, SEQ ID No:204, SEQ ID No:205, SEQ ID No:206 and SEQ ID No:207, or shown in SEQ ID No:203, SEQ ID No:205, SEQ ID No:206, SEQ ID No:207 and SEQ ID No:208 nucleotide sequence.

4,, it is characterized in that in the step (3), described yeast secreted expression signal peptide sequence is the signal peptide sequence of yeast saccharomyces cerevisiae α-mating factor according to the described separation method of claim 2.

5, according to the described separation method of claim 2, it is characterized in that in the step (3), the restriction enzyme site of described double digestion is EcoRI and XbaI, the restriction enzyme site that described three enzymes are cut is EcoRI, XhoI and XbaI.

6,, it is characterized in that in the step (4) that described enzyme is cut and handled is to handle plasmid with restriction enzyme NotI and calf intestinal alkaline phosphatase according to the described separation method of claim 2.

7,, it is characterized in that being added with anthocyanidin on the screening flat board of described screening transformant in the step (4) according to the described separation method of claim 2.

8, utilize claim 2 methods to separate the foot and mouth disease virus series of variation that obtains, its aminoacid sequence is shown in SEQ ID NO:101～200.

9, described according to Claim 8 foot and mouth disease virus series of variation, the nucleotide sequence that it is characterized in that encode such amino acid sequences is shown in SEQ ID NO:1～100.