CN101624576A - Mycobacterium cosmeticum with capacity of degrading benzene compound and application thereof - Google Patents
Mycobacterium cosmeticum with capacity of degrading benzene compound and application thereof Download PDFInfo
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- CN101624576A CN101624576A CN200910096028A CN200910096028A CN101624576A CN 101624576 A CN101624576 A CN 101624576A CN 200910096028 A CN200910096028 A CN 200910096028A CN 200910096028 A CN200910096028 A CN 200910096028A CN 101624576 A CN101624576 A CN 101624576A
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- benzene
- benzene compounds
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- 241001420379 Mycobacterium cosmeticum Species 0.000 title claims abstract description 34
- 230000000593 degrading effect Effects 0.000 title claims abstract description 22
- -1 benzene compound Chemical class 0.000 title abstract 2
- 150000001555 benzenes Chemical class 0.000 claims abstract description 61
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 66
- YNQLUTRBYVCPMQ-UHFFFAOYSA-N Ethylbenzene Chemical compound CCC1=CC=CC=C1 YNQLUTRBYVCPMQ-UHFFFAOYSA-N 0.000 claims description 38
- 241000186359 Mycobacterium Species 0.000 claims description 21
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- 239000002351 wastewater Substances 0.000 claims description 12
- 239000002912 waste gas Substances 0.000 claims description 6
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
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- 238000006731 degradation reaction Methods 0.000 description 31
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- GZWHKZMMMSLXNV-UHFFFAOYSA-N benzene;1,2-xylene Chemical compound C1=CC=CC=C1.CC1=CC=CC=C1C GZWHKZMMMSLXNV-UHFFFAOYSA-N 0.000 description 1
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Images
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention provides a mycobacterium cosmeticum byf-4 with the capacity of degrading a benzene compound and an application thereof. The mycobacterium cosmeticum byf-4 is stored in Chinese typical culture storage center which has an address: Wuhan University Wuhan China, 430074, a storage date: Oct. 29, 2008 and a storage number: CCTCC No. M 208180. The invention has the advantages that the strain can rapidly degrade various benzene compounds with high efficiency and has an important meaning to the engineering practice of biologically purifying contaminants caused by the benzene compounds and the derivatives thereof.
Description
(1) technical field
The present invention relates to mycobacterium (Mycobacterium cosmeticum) byf-4 and application thereof that a strain has the degrading benzene compounds ability.
(2) background technology
Benzene compounds is the volatile monocycle aromatic compounds of a class, comprise benzene, toluene, ethylbenzene, dimethylbenzene etc., abbreviate BTEX as, being widely used in the production process of industry such as oil, chemical industry, agricultural chemicals, weaving, papermaking, paint, shoemaking, is a class toxic compounds distributed more widely in the environment.BTEX has " three cause effect ", is listed in priority pollutants by many countries, and is confirmed to be strong carcinogen.The Pollution control technology of BTEX seems very important and urgent in research and development waste water, the waste gas.At present, biodegradation technique is one of most effectual way of this class material of degraded.
One of key that adopts toxic compounds such as BTEX in biological method purification waste water, the waste gas is to obtain strain excellent with efficient degradation BTEX ability.People have isolated many strains monocycle aromatic compounds degradation bacteria, mainly contain pseudomonas (Pseudomonas), acinetobacter calcoaceticus (Acinetobacter), rhodococcus (Rhodococcus), the logical Salmonella (Ralstonia) in Rolls, Alcaligenes (Alcaligenes), Flavobacterium (Flavobacterium), spore Saksenaea fungi (Cladophialophora), Nocardia bacteria (Nocardia) etc.These degradation bacteria strains only can be degraded a certain or two kinds of benzene compounds, and the degradation efficiency of most of bacterial strains remains further to be improved, and can realize all that to each material in the BTEX mixture bacterial strain of efficient degradation is actually rare.
(3) summary of the invention
The purpose of this invention is to provide plant height effect, the bacterial classification and the application thereof of degrading benzene compounds fast.
The technical solution used in the present invention is:
Mycobacterium (Mycobacterium cosmeticum) byf-4 with degrading benzene compounds ability, be preserved in Chinese typical culture collection center, the address: Chinese Wuhan Wuhan University, 430074, preservation date: on October 29th, 2008, deposit number: CCTCC No:M 208180.Degradation bacteria strains of the present invention can be realized efficient degradation BTEX mixture, therefore, can bring into play more important role in actual applications.By retrieval, the report of using Mycobacteriumcosmeticum degraded BTEX under aerobic condition is not arranged at present, so the discovery of this degradation bacteria is significant to the engineering practice of BTEX pollutant biopurification.
Described Mycobacterium cosmeticum byf-4 (CCTCC No:M 208180) derives from the active sludge in Zhenghai, Sinopec Zhejiang refinery sewage disposal plant aeration tank, obtains through domestication, separation, purifying.
This bacterial strain is light oyster white in solid medium, circle, and neat in edge, smooth moistening, diameter is 0.5～1mm; The form of thalline is a tyrothricin, and length is about 2 μ m; No gemma; Aerobic, Gram-negative, catalytic oxidation enzyme feminine gender can be a sole carbon source with BTEX mixture or one matter wherein, its physiological and biochemical property is as shown in table 1.The 16S rDNA sequence of Mycobacterium cosmeticumbyf-4CCTCC No:M 208180 is as follows:
ttaacacatgcaagtcgaacggaaaggcccttcggggtgctcgagtggcgaacgggtgagtaacacgtgggtgatctgccctgcactttgggataagcctgggaaactgggtctaataccgaataggactccggccttcatgggttggggtggaaagcttttgcggtgtgggatgggcccgcggcctatcagcttgttggtggggtaatggcctaccaaggcgacgacgggtagccggcctgagagggtgaccggccacactgggactgagatacggcccagactcctacgggaggcagcagtggggaatattgcacaatgggcgcaagcctgatgcagcgacgccgcgtgagggatgacggccttcgggttgtaaacctctttcagcacagacgaagcgcaagtgacggtatgtgcagaagaaggaccggccaactacgtgccagcagccgcggtaatacgtagggtccgagcgttgtccggaattactgggcgtaaagagctcgtaggtggtttgtcgcgttgttcgtgaaaactcacagcttaactgtgggcgtgcgggcgatacgggcagactggagtactgcaggggagactggaattcctggtgtagcggtggaatgcgcagatatcaggaggaacaccggtggcgaaggcgggtctctgggcagtaactgacgctgaggagcgaaagcgtggggagcgaacaggattagataccctggtagtccacgccgtaaacggtgggtactaggtgtgggtttccttccttgggatccgtgccgtagctaacgcattaagtaccccgcctggggagtacggccgcaaggctaaaactcaaaggaattgacgggggcccgcacaagcggcggagcatgtggattaattcgatgcaacgcgaagaaccttacctgggtttgacatgcacaggacgctggtagagatatcagttcccttgtggcctgtgtgcaggtggtgcatggctgtcgtcagctcgtgtcgtgagatgttgggttaagtcccgcaacgagcgcaacccttgtcctatgttgccagcgggttatgccggggactcgtaggagactgccggggtcaactcggaggaaggtggggatgacgtcaagtcatcatgccccttatgtccagggcttcacacatgctacaatggccggtacaaagggctgcgatgccgtgaggtggagcgaatccttgtaaagccggtctcagttcggatcggggtctgcaactcgaccccgtgaagtcggagtcgctagtaatcgcagatcagcaacgctgcggtgaatacgttcccgggccttgtacacaccgcccgtcacgtcatgaaagtcggtaacacccgaagccggtggcctaaccccttgtgggagggagccgtcgaaggtgggatcggcgattgggacgaagtcgaacaa
This degradation bacteria strains physiological biochemical property sees Table 1:
The physiological and biochemical property of table 1:Mycobacterium cosmeticum byf-4
| Experimental project | The result |
| M.R (methyl red) | ??- |
| The V.P experiment | ??- |
| Amylolytic enzyme | ??- |
| The indoles experiment | ??+ |
| Nitrate reduction | ??+ |
| The gelatin experiment | ??+ |
| ??H 2The S experiment | ??- |
| Glucose | ??+ |
| Lactose | ??- |
Note: "+" expression can utilize or reacting positive; "-" expression can not utilize or reaction negative the invention still further relates to the application of described mycobacterium byf-4 in degrading benzene compounds and derivative thereof.
Described mycobacterium byf-4 can be used for degrading benzene compounds and derivative thereof in trade effluent or the waste gas.When being used for the benzene compounds of degrading waste water, mycobacterium byf-4 is seeded in the waste water that contains benzene compounds, under the condition of suitable mycobacterium growth, cultivates and get final product according to this area ordinary method; When being used for degrading the benzene compounds of waste gas,, under the condition of suitable mycobacterium growth, waste gas being fed handle in the bacterium liquid of mycobacterium byf-4 and get final product according to this area ordinary method.
Concrete, described being applied as: mycobacterium byf-4 thalline is suspended in the waste water that contains benzene compounds and derivative thereof, under 15～35 ℃, pH5～9 conditions, cultivates benzene compounds in the degrading waste water and derivative thereof.
Preferably, described being applied as: mycobacterium byf-4 thalline is suspended in the waste water that contains benzene compounds and derivative thereof, under 30 ℃, pH7 condition, cultivates benzene compounds in the degrading waste water and derivative thereof.
Described benzene compounds is preferably one of following or wherein two or more mixtures: benzene, toluene, ethylbenzene, o-Xylol.
Beneficial effect of the present invention is mainly reflected in: the bacterial strain of the present invention all kinds of benzene compounds of can degrading efficiently, fast, the engineering practice of the biopurification of the pollutent that causes for benzene compounds and derivative thereof is significant.
(4) description of drawings
Fig. 1 is Mycobacterium cosmeticum byf-4 microscope and scanning, transmission electron microscope photo (a, gramstaining microscope picture; B, the scanning electron microscope picture; C, the transmission electron microscope picture);
Fig. 2 is the phylogeny tree graph of Mycobacterium cosmeticum byf-4;
Fig. 3 be under the differing temps bacterium Mycobacterium cosmeticum byf-4 to the degradation curve figure of BTEX;
Fig. 4 be under the different pH bacterium Mycobacterium cosmeticum byf-4 to the degradation curve of BTEX;
Fig. 5 is the degradation curve figure of bacterium Mycobacterium cosmeticum byf-4 to the benzene of different starting point concentrations;
Fig. 6 is the degradation curve figure of bacterium Mycobacterium cosmeticum byf-4 to the toluene of different starting point concentrations;
Fig. 7 is the degradation curve figure of bacterium Mycobacterium cosmeticum byf-4 to the ethylbenzene of different starting point concentrations;
Fig. 8 is the degradation curve figure of bacterium Mycobacterium cosmeticum byf-4 to the o-Xylol of different starting point concentrations;
Fig. 9 is that bacterium Mycobacterium cosmeticum byf-4 is to mixing the degradation curve figure of substrate.
(5) embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this (as not specifying that percentage concentration all refers to mass percent concentration among the embodiment):
Separation and the purifying of the separation of embodiment 1:Mycobacterium cosmeticum byf-4, purifying and evaluation 1.Mycobacterium cosmeticum byf-4 thereof
Mycobacterium cosmeticum byf-4 takes a sample from the active sludge of Zhenghai, Zhejiang refinery sewage disposal plant aeration tank, a strain Gram-negative bacteria that obtains through domestication, separation and purifying.Concrete steps are as follows:
(1) the directed domestication of mud
Get the active sludge of Zhenghai, Zhejiang refinery sewage disposal plant aeration tank.Active sludge carries out the directed domestication of benzene compounds (BTEX), and per 5 days is an acclimation period, tames 3 months, until active sludge benzene compounds is realized the stability and high efficiency degraded.
(2) screening of bacterial strain and purifying
Utilize benzene series thing degradation bacteria screening culture medium that active sludge is carried out bacterial strain screening.Benzene series thing degradation bacteria screening culture medium preparation: KH
2PO
4, 0.5g; K
2HPO
4, 0.5g; NaCl, 0.01g; NH
4Cl, 1g; MgCl
26H
2O, 0.2g; CaCl
2, 0.02g; MnSO
4, 0.339mg; ZnSO
4, 0.428mg; Ammonium molybdate, 0.347mg; Agar, 15g; Distilled water, 1000ml; PH 7.2～7.4.The benzene series thing adds respectively in addition: benzene, 200mg/l; Toluene, 200mg/l; Ethylbenzene, 200mg/l; O-Xylol: 200mg/l.
According to the ordinary method of strains separation purifying, to cultivate 2～3 days for 28 ℃ at constant incubator, the single bacterium colony that grows on the picking flat board obtains the pure bacterium colony of many strains.
The many strains benzene series thing degradation bacteria that screens is investigated the removal effect of bacterial strain to benzene compounds in containing the minimal medium that benzene compounds is a sole carbon source, by the contrast with blank assay, the result has obtained the strain b-yf-4 of a high-efficiency degradation benzene compounds.Used medium component is: benzene 0.1g/L, toluene 0.1g/L, ethylbenzene 0.1g/L, o-Xylol 0.1g/L, NH
4Cl 0.1g/L, MgCl
26H
2O 0.08g/L, K
2HPO
40.25g/L, CaCl
22H
2O 0.1g/L, solvent are water, and pH 7.2～7.4.
2.Mycobacterium the 16S rDNA of cosmeticum byf-4 identifies
Identify by 16S rRNA sequential analysis and Biolog microbial identification system, determine that the byf-4 bacterium is Mycobacterium cosmeticum.Concrete steps are as follows:
Adopt the centrifugal environmental sample DNA of 3S post to reclaim the DNA that test kit (V2.2, Shanghai Shenergy Biocolor BioScience ﹠ Technology Company) extracts Mycobacterium cosmeticum byf-4,4 ℃ of preservations.As the template of PCR reaction, design primer then, the complete sequence DNA of amplification Mycobacteriumcosmeticum byf-4, the upstream and downstream primer sequence is as follows respectively:
BSF8/20：5′-AGAGT?TTGAT?CCTGG?CTCAG-3′
BSR1541/20：5′-AAGGA?GGTGA?TCCAGCCGCA-3′
The PCR response procedures is set at: 94 ℃ of pre-sex change 4min of elder generation; 94 ℃ of sex change 1min then, 59 ℃ of annealing 1min, 72 ℃ are extended 1.5min, circulate 35 cycles; 72 ℃ are extended 10min then; Last 4 ℃ keep 10min.The PCR product is checked order (Shanghai English fine horse), sequencing result is seen SEQID No.1.
The 16S rDNA sequence of byf-4 is uploaded to Genbank, obtain the accession number FJ169473 of Genbank, carry out homology relatively with the gene order among the Genbank simultaneously, find that it belongs to Mycobacterium and belongs to, foundation by phylogenetic tree then, byf-4 may be Mycobacterium cosmeticum, and Fig. 2 is the phylogeny tree graph of this bacterium, and Fig. 1 is the microscope of this bacterium and scanning, transmission electron microscope photo.In order further to determine qualification result,, determine that finally byf-4 is Mycobacterium cosmeticum by the analysis of every Physiology and biochemistry experiment and Biolog microbial identification system.
Embodiment 2:Mycobacterium cosmeticum byf-4 detects the BTEX biodegradability
Under the differing temps bacterium Mycobacterium cosmeticum byf-4 to the biodegradation character of BTEX
Under differing temps, implement the degradation experiment of Mycobacterium cosmeticum byf-4 to BTEX, find that it is 25～35 ℃ of abilities with higher degrading benzene compounds, from the practical application angle, 30 ℃ is the most suitable temperature, this moment, clearance was the highest, and concrete implementation step is as follows:
(final concentration consists of: benzene 50mg/L, toluene 50mg/L, ethylbenzene 50mg/L, o-Xylol 50mg/L, NH containing the inorganic medium that benzene compounds is a sole carbon source
4Cl 0.1g/L, MgCl
26H
2O 0.08g/L, K
2HPO
40.25g/L, CaCl
22H
2O 0.1g/L, solvent are water, and pH 7.2～7.4) add the bacteria suspension of the byf-4 of fresh culture, OD
600Be 0.2, inoculum size is 1ml/50ml, and branch is got 50ml substratum branch and is filled in the saline bottle of 5 250ml, places 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃ shaking table 160rpm to cultivate respectively; Prepare 5 same substratum that do not connect bacterium in addition and place corresponding temperature to cultivate simultaneously respectively, as blank.After cultivating 48h, the concentration of sampling vapor detection benzene compounds is measured the nectar degree (OD of bacterial strain simultaneously
600), draw benzene series thing clearance graphic representation.
The result shows that when temperature was 15 ℃, bacterial strain was lower to the clearance of benzene compounds as shown in Figure 3.Along with the raising gradually of temperature, bacterial strain also improves the clearance of benzene compounds thereupon; When being 25～35 ℃, temperature is this bacterial strain temperature of suitable growth comparatively, the growth of bacterial strain and the clearance of benzene series thing all reached higher value; Reached maximum value during 30 ℃ of temperature, 48h is respectively benzene to the clearance of benzene series thing, and 98.2%; Toluene, 95.6%; Ethylbenzene, 78.3%; O-Xylol, 68.4%.Along with the further raising of temperature, the growth of bacterial strain and degradation capability begin to descend.
2. bacterium Mycobacterium cosmeticum byf-4 is to the degradation characteristic of BTEX under the different pH
Implement the degradation experiment of Mycobacterium cosmeticum byf-4 to BTEX under different pH, the result shows that the growth degraded pH value of its best is 7, and specific embodiments is as follows:
The minimal medium that adds 50ml equivalent in the saline bottle of 5 250ml, benzene compounds are as sole carbon source, and the substratum final concentration is formed the same, regulates pH to 5,6,7,8,9 respectively; Prepare 5 bottles of identical substratum simultaneously as blank.All inoculate the byf-4 bacteria suspension of 1ml fresh culture, OD
600Be 0.2, at 30 ℃, 160rpm cultivates down.At 0h, 48h takes a sample respectively, the benzene compounds change in concentration in the detection substratum and the nectar degree (OD of bacterial strain
600), draw benzene series thing clearance graphic representation.
The result is 5,9 o'clock at pH as shown in Figure 4, and it is relatively poor that byf-4 removes efficient to the benzene series thing.In pH was 5 nutrient solution, solution is clarification, and was the same with blank solution, shows that strain growth is very bad.Be under 5～9 the condition at pH, bacterium can both be the sole carbon source growth with the benzene compounds, just growth and degradation rate are different, when pH is, growth and the degradation rate of strain b-yf-4 are the fastest, and the clearance of benzene, toluene, ethylbenzene, o-Xylol 48h is respectively 97.4%, 95.8%, 86.5%, 60.5%.
3. bacterium Mycobacterium cosmeticum byf-4 is to the degraded situation of the BTEX of different starting point concentrations
Under different benzene series thing starting point concentrations, implement the degraded of byf-4, found that byf-4 is 500mg/l, 300mg/l, 125mg/l, 200mg/l to the tolerance concentration of benzene, toluene, ethylbenzene and o-Xylol benzene, toluene, ethylbenzene and four kinds of benzene series things of o-Xylol.Specific embodiments is as follows:
(other component final concentrations are the minimal medium of adding 50ml except that carbon source: NH in the saline bottle of 250ml
4Cl 0.1g/L, MgCl
26H
2O 0.08g/L, K
2HPO
40.25g/L, CaCl
22H
2O 0.1g/L, solvent are water, and pH 7.2～7.4), benzene compounds is as sole carbon source.The final concentration of benzene is respectively 25,50,150,300,500mg/l; The final concentration of toluene is respectively 25,50,150,200,300mg/l; The final concentration of ethylbenzene is respectively 25,50,75,100,125mg/l; The final concentration of o-Xylol is respectively 25,50,100,150,200mg/l.Inoculate equivalent 1mlOD respectively
600Be 0.2 bacteria suspension, 30 ℃, 160rpm cultivates, and the degradation curve figure of byf-4 to the benzene series thing drawn in the certain interval of time sampling.
From Fig. 5～Fig. 8 as can be seen when benzene concentration is lower than 500mg/l, the byf-4 benzene of can degrading apace, bacterial growth is good, and when being higher than 500mg/l, bacterial strain is subjected to strongly inhibited; Owing to be subjected to the solubility limit of toluene in water, the maximum concentration of toluene is only accomplished 300mg/l, when toluene concentration is in 300mg/l, and the byf-4 toluene of all can degrading apace, bacterial growth is good; Byf-4 will be slower than benzene and toluene to the degradation speed of ethylbenzene, o-Xylol, and when ethylbenzene, o-Xylol concentration during respectively greater than 125mg/l, 150mg/l, the growth of byf-4 degraded is suppressed, can not efficient degradation.
4. bacterium Mycobacterium cosmeticum byf-4 is to mixing the degraded situation of substrate
Serving as to mix substrate with benzene, toluene, ethylbenzene, four kinds of benzene series things of o-Xylol, implement the degradation characteristic of bacterium Mycobacterium cosmeticum byf-4 to BTEX, the result shows that its degraded complexity to four kinds of materials is o-Xylol＞ethylbenzene＞benzene＞toluene.Specific embodiments is as follows:
(final concentration consists of the minimal medium of adding 50ml: benzene 50mg/L, toluene 50mg/L, ethylbenzene 50mg/L, o-Xylol 50mg/L, NH in the saline bottle of 250ml
4Cl 0.1g/L, MgCl
26H
2O 0.08g/L, K
2HPO
40.25g/L, CaCl
22H
2O 0.1g/L, solvent are water, and pH 7.2～7.4), benzene compounds is as sole carbon source, and total concn all is 50mg/L respectively.Inoculation 1ml OD
600Be 0.2 bacteria suspension, 30 ℃, 160rpm cultivates.Sampling at set intervals detects benzene compounds concentration and nectar degree (OD in the substratum
600), up to degraded fully.Draw the degradation curve and the strain growth figure of benzene series thing.
The result as shown in Figure 9, at the degraded initial stage, bacterial strain has lag phase about 5～10h to the degraded of benzene series thing, strain growth is slow simultaneously; Behind 15h, bacterial strain begins to adapt to this environment, enters logarithmic phase, and the benzene series thing is degraded fast, and as can be seen from the figure, byf-4 will be obviously faster than benzene, ethylbenzene and o-Xylol to the removal speed of toluene; When 35h, toluene is degraded fully, and benzene, ethylbenzene, o-Xylol successively have been degraded at 41h, 45h, 50h.The nectar degree of bacterial strain reaches maximum value at 30～45h, and bacterial strain enters the paracme behind the 50h.
SEQUENCE?LISTING
＜110〉Zhejiang Polytechnical University
＜120〉have the mycobacterium byf-4 and the application thereof of degrading benzene compounds ability
<130>
<160>3
<170>PatentIn?version?3.2
<210>1
<211>1433
<212>DNA
<213>Mycobacterium?cosmeticum
<400>1
ttaacacatg?caagtcgaac?ggaaaggccc?ttcggggtgc?tcgagtggcg?aacgggtgag???60
taacacgtgg?gtgatctgcc?ctgcactttg?ggataagcct?gggaaactgg?gtctaatacc??120
gaataggact?ccggccttca?tgggttgggg?tggaaagctt?ttgcggtgtg?ggatgggccc??180
gcggcctatc?agcttgttgg?tggggtaatg?gcctaccaag?gcgacgacgg?gtagccggcc??240
tgagagggtg?accggccaca?ctgggactga?gatacggccc?agactcctac?gggaggcagc??300
agtggggaat?attgcacaat?gggcgcaagc?ctgatgcagc?gacgccgcgt?gagggatgac??360
ggccttcggg?ttgtaaacct?ctttcagcac?agacgaagcg?caagtgacgg?tatgtgcaga??420
agaaggaccg?gccaactacg?tgccagcagc?cgcggtaata?cgtagggtcc?gagcgttgtc??480
cggaattact?gggcgtaaag?agctcgtagg?tggtttgtcg?cgttgttcgt?gaaaactcac??540
agcttaactg?tgggcgtgcg?ggcgatacgg?gcagactgga?gtactgcagg?ggagactgga??600
attcctggtg?tagcggtgga?atgcgcagat?atcaggagga?acaccggtgg?cgaaggcggg??660
tctctgggca?gtaactgacg?ctgaggagcg?aaagcgtggg?gagcgaacag?gattagatac??720
cctggtagtc?cacgccgtaa?acggtgggta?ctaggtgtgg?gtttccttcc?ttgggatccg??780
tgccgtagct?aacgcattaa?gtaccccgcc?tggggagtac?ggccgcaagg?ctaaaactca??840
aaggaattga?cgggggcccg?cacaagcggc?ggagcatgtg?gattaattcg?atgcaacgcg??900
aagaacctta?cctgggtttg?acatgcacag?gacgctggta?gagatatcag?ttcccttgtg??960
gcctgtgtgc?aggtggtgca?tggctgtcgt?cagctcgtgt?cgtgagatgt?tgggttaagt??1020
cccgcaacga?gcgcaaccct?tgtcctatgt?tgccagcggg?ttatgccggg?gactcgtagg??1080
agactgccgg?ggtcaactcg?gaggaaggtg?gggatgacgt?caagtcatca?tgccccttat??1140
gtccagggct?tcacacatgc?tacaatggcc?ggtacaaagg?gctgcgatgc?cgtgaggtgg??1200
agcgaatcct?tgtaaagccg?gtctcagttc?ggatcggggt?ctgcaactcg?accccgtgaa??1260
gtcggagtcg?ctagtaatcg?cagatcagca?acgctgcggt?gaatacgttc?ccgggccttg??1320
tacacaccgc?ccgtcacgtc?atgaaagtcg?gtaacacccg?aagccggtgg?cctaacccct??1380
tgtgggaggg?agccgtcgaa?ggtgggatcg?gcgattggga?cgaagtcgaa?caa?????????1433
<210>2
<211>20
<212>DNA
<213>Unknown
<220>
＜223〉artificial sequence
<400>2
agagtttgat?cctggctcag????20
<210>3
<211>20
<212>DNA
<213>Unknown
<220>
＜223〉artificial sequence
<400>3
aaggaggtga?tccagccgca????20
Claims (6)
1. mycobacterium (Mycobacterium cosmeticum) byf-4 that has the degrading benzene compounds ability, be preserved in Chinese typical culture collection center, address: Chinese Wuhan Wuhan University, 430074, preservation date: on October 29th, 2008, deposit number: CCTCC No:M208180.
2. the application of mycobacterium byf-4 as claimed in claim 1 in degrading benzene compounds and derivative thereof.
3. application as claimed in claim 2 is characterized in that described mycobacterium byf-4 be used for the degrading benzene compounds and the derivative thereof of trade effluent or waste gas.
4. application as claimed in claim 2, it is characterized in that described being applied as: mycobacterium byf-4 thalline is suspended in the waste water that contains benzene compounds and derivative thereof, under 15～35 ℃, pH5～9 conditions, cultivate benzene compounds in the degrading waste water and derivative thereof.
5. application as claimed in claim 4, it is characterized in that described being applied as: mycobacterium byf-4 thalline is suspended in the waste water that contains benzene compounds and derivative thereof, under 30 ℃, pH7 condition, cultivate benzene compounds in the degrading waste water and derivative thereof.
6. as the described application of one of claim 2～5, it is characterized in that described benzene compounds is one of following or wherein two or more mixture: benzene, toluene, ethylbenzene, o-Xylol.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899271A (en) * | 2012-09-29 | 2013-01-30 | 轻工业环境保护研究所 | Mycobacterium 16F for efficiently degrading polycyclic aromatic hydrocarbons and benzene organic matters and application thereof |
| CN102961962A (en) * | 2012-12-14 | 2013-03-13 | 中国矿业大学 | Technology for processing indissolvable and nondegradable organic waste gas by integrated composite organisms |
| CN115044506A (en) * | 2022-06-15 | 2022-09-13 | 仲恺农业工程学院 | Actinomycete FAD02 capable of decomposing fusaric acid and application thereof |
-
2009
- 2009-01-23 CN CN2009100960289A patent/CN101624576B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899271A (en) * | 2012-09-29 | 2013-01-30 | 轻工业环境保护研究所 | Mycobacterium 16F for efficiently degrading polycyclic aromatic hydrocarbons and benzene organic matters and application thereof |
| CN102899271B (en) * | 2012-09-29 | 2014-08-06 | 轻工业环境保护研究所 | Mycobacterium 16F for efficiently degrading polycyclic aromatic hydrocarbons and benzene organic matters and application thereof |
| CN102961962A (en) * | 2012-12-14 | 2013-03-13 | 中国矿业大学 | Technology for processing indissolvable and nondegradable organic waste gas by integrated composite organisms |
| CN115044506A (en) * | 2022-06-15 | 2022-09-13 | 仲恺农业工程学院 | Actinomycete FAD02 capable of decomposing fusaric acid and application thereof |
| CN115044506B (en) * | 2022-06-15 | 2023-05-30 | 仲恺农业工程学院 | Actinomycete FAD02 for decomposing fusaric acid and application thereof |
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