CN101603959A - A kind of method of fast measuring similarity of pork flavor - Google Patents

Abstract

The invention discloses a kind of method of fast measuring similarity of pork flavor, utilize the similarity of smell signature analyzer FOX4000 fast measuring allotment type liquid pork essence, its step has: the analytical parameters of A. setting smell signature analyzer FOX4000; B. allotment type liquid pork essence standard specimen and allotment type liquid pork essence spline are carried out analytical test by smell signature analyzer FOX4000, form with column diagram and radar map is analyzed comparison to the fragrance profile between described allotment type liquid pork essence standard specimen and the allotment type liquid pork essence spline, calculates the similarity of allotment type liquid pork essence spline at last according to formula.The inventive method is simple, quick, and objective data can be provided, and has overcome the defective of subjective appreciation, and crucial effects has been played in the quality control of pork essence.

Description

A kind of method of fast measuring similarity of pork flavor

Technical field

The present invention relates to a kind of assay method of meat flavor similarity, more particularly relate to a kind of method of utilizing smell signature analyzer (Electronic Nose) fast measuring similarity of pork flavor.

Background technology

Meat flavor is a kind of important food additives, be widely used in numerous leisure food such as instant noodles, meat products, Frozen Prepared Food, dilated food, can effectively improve the local flavor and the mouthfeel of product, the quality of end product is played crucial effects.And a kind of as meat flavor of allotment type liquid pork essence, the demand on market is higher than other kind far away, is subjected to deeply that the consumer's is ambiguous.Research around meat flavor all has relevant report both at home and abroad.But wherein the great majority report has only been introduced the THE PREPARATION OF MEAT FLAVOR method, is lacking systematic research aspect the essence similarity evaluation.For a long time, the researcher is the means that subjective appreciation is passed judgment on as the essence similarity mostly, owing to be subjected to the interference of factors such as estimator's mood, environment in the evaluation procedure easily, causes the result objective inadequately, accurate.This method can't satisfy the quality control requirement in the food production fully, has seriously restricted the development of spices and essence industry to a certain extent.In recent years, biology, computer and bionics are rapidly developed, a kind of can the objective determination smell and other volatile substance instrument---Electronic Nose is come out thereupon.It rolls into one modern high technology, the function of mimic biology sense organ, energy is differentiated and is judged the various samples such as gas, liquid and solid that have smell, is " green " bionical detection technique of a kind of novelty, and analyzes quick, easy, can in time provide product for information about, compare with the sense of smell judgement of humans and animals, its mensuration is more objective, is not subjected to the influence of biosome subjective factor, the result is more reliable, and reappearance is better.For example, smell signature analyzer FOX4000 can analyze comparison with the form of column diagram and radar map to the fragrance profile of allotment type liquid pork essence spline and allotment type liquid pork essence standard specimen by the response signal of inner 18 sensors is carried out mathematics manipulation.

Summary of the invention

Technical matters to be solved by this invention is to utilize smell signature analyzer FOX4000 that a kind of method of fast measuring allotment type liquid similarity of pork flavor is provided.

Technical solution of the present invention: 1. the method for a fast measuring similarity of pork flavor, utilize the similarity of smell signature analyzer FOX4000 fast measuring allotment type liquid pork essence, comprise the following steps:

The analytical parameters of A, setting smell signature analyzer FOX4000, wherein: carrier gas is synthetic dry air, flow velocity 150mL/min, the generation time that head space produces parameter is 120s, 60 ℃ of temperature of generation, agitation speed 500r/min, the volume injected of head space injection parameters is 60 ℃ of 500 μ L, injection speed 500 μ L/s, entry needle cumulative volume 2.5mL, entry needle temperature, and acquisition time getparms is 120s, lag time 600s;

B, allotment type liquid pork essence standard specimen and allotment type liquid pork essence spline are respectively taken by weighing 0.1 gram, place sample bottle respectively, the analytical parameters of A setting carries out analytical test by smell signature analyzer FOX4000 to described allotment type liquid pork essence standard specimen and described allotment type liquid pork essence spline set by step, form with column diagram and radar map is analyzed comparison to the fragrance profile between described allotment type liquid pork essence standard specimen and the allotment type liquid pork essence spline, calculates the similarity of allotment type liquid pork essence spline at last according to formula (I):

$\mathrm{SI}(\%)=(1-\frac{\sqrt{\underset{i=1}{\overset{18}{\mathrm{\Σ}}}{\left(\right|\mathrm{Ai}|-|\mathrm{Bi}\left|\right)}^2}}{\sqrt{\frac{1}{2}\underset{i=1}{\overset{18}{\mathrm{\Σ}}}{\left(\right|\mathrm{Ai}|+|\mathrm{Bi}\left|\right)}^2}})\×100---\left(I\right)$

Ai represents the response signal value of allotment type liquid pork essence standard specimen on i sensor in the formula (I), and Bi represents the response signal value of allotment type liquid pork essence spline on i sensor, and SI represents similarity.

Beneficial effect of the present invention: the present invention measures the similarity of allotment type liquid pork essence by smell signature analyzer FOX4000 (Electronic Nose), if the similarity (SI) of allotment type liquid pork essence spline was greater than 90% o'clock, can think allotment type liquid pork essence spline and allotment type liquid pork essence standard specimen fragrance basically identical, and allotment type liquid pork essence spline and allotment type liquid pork essence standard specimen carry out application test respectively by same ratio in application product after, the fragrance and the mouthfeel of end product almost are equal to, and obtain consumer's generally approval.If the similarity (SI) of allotment type liquid pork essence spline was less than 90% o'clock, then allotment type liquid pork essence spline and allotment type liquid pork essence standard specimen fragrance are deposited a certain distance, and the perfuming effect of essence is also undesirable in the reality of using this product.The inventive method is simple, speed is fast, can reflect difference degree between allotment type liquid pork essence spline and the allotment type liquid pork essence standard specimen intuitively by column diagram and radar map, and can provide objective data, overcome the defective of subjective appreciation, vital role has been played in the quality control of pork essence.

Description of drawings

Figure 1A is that column diagram is compared in the analysis of allotment type liquid pork essence N1 and allotment type liquid pork essence standard specimen N;

Figure 1B is that radar map is compared in the analysis of allotment type liquid pork essence N1 and allotment type liquid pork essence standard specimen N;

Fig. 2 A is that column diagram is compared in the analysis of allotment type liquid pork essence N2 and allotment type liquid pork essence standard specimen N;

Fig. 2 B is that radar map is compared in the analysis of allotment type liquid pork essence N2 and allotment type liquid pork essence standard specimen N;

Fig. 3 A is that column diagram is compared in the analysis of allotment type liquid pork essence N3 and allotment type liquid pork essence standard specimen N;

Fig. 3 B is that radar map is compared in the analysis of allotment type liquid pork essence N3 and allotment type liquid pork essence standard specimen N.

Embodiment

By embodiment the present invention is described in further detail, a kind of method of fast measuring similarity of pork flavor is utilized the similarity of smell signature analyzer FOX4000 fast measuring allotment type liquid pork essence, comprises the following steps:

The analytical parameters of A, setting smell signature analyzer FOX4000, wherein: carrier gas is synthetic dry air, flow velocity 150mL/min, the generation time that head space produces parameter is 120s, 60 ℃ of temperature of generation, agitation speed 500r/min, the volume injected of head space injection parameters is 60 ℃ of 500 μ L, injection speed 500 μ L/s, entry needle cumulative volume 2.5mL, entry needle temperature, and acquisition time getparms is 120s, lag time 600s;

B, allotment type liquid pork essence standard specimen and allotment type liquid pork essence spline are respectively taken by weighing 0.1 gram, place sample bottle respectively, the analytical parameters of A setting carries out analytical test by smell signature analyzer FOX4000 to described allotment type liquid pork essence standard specimen and described allotment type liquid pork essence spline set by step, form with column diagram and radar map is analyzed comparison to the fragrance profile between described allotment type liquid pork essence standard specimen and the allotment type liquid pork essence spline, calculates the similarity of allotment type liquid pork essence spline at last according to formula (I):

$\mathrm{SI}(\%)=(1-\frac{\sqrt{\underset{i=1}{\overset{18}{\mathrm{\Σ}}}{\left(\right|\mathrm{Ai}|-|\mathrm{Bi}\left|\right)}^2}}{\sqrt{\frac{1}{2}\underset{i=1}{\overset{18}{\mathrm{\Σ}}}{\left(\right|\mathrm{Ai}|+|\mathrm{Bi}\left|\right)}^2}})\×100---\left(I\right)$

Ai represents the response signal value of allotment type liquid pork essence standard specimen on i sensor in the formula (I), and Bi represents the response signal value of allotment type liquid pork essence spline on i sensor, and SI represents similarity.

Embodiment 1, the mensuration of allotment type liquid pork essence spline N1 and allotment type liquid pork essence standard specimen N similarity

1%2-methyl-3-the thiomethylfuran that in beaker, adds 1 (parts by weight) successively, 2% furanone of 5 (parts by weight), 10%3-sulfydryl-2-the butanone of 1 (parts by weight), 10% butyric acid of 1 (parts by weight), 2 (parts by weight) 1%2,3, the 5-trimethylpyrazine, 1% cassia oil of 1 (parts by weight), 1% anisic aldehyde of 1 (parts by weight), 1% pair of (2-methyl-3-furyl) disulfide of 1 (parts by weight), 10%4-methyl-5-the hydroxy ethylthiazole of 2 (parts by weight), 1% ethyl maltol of 12 (parts by weight), 1% difurfuryl dithioether of 2 (parts by weight), 1% furfuryl mercaptan of 1 (parts by weight), the 1%3-methyl mercapto propyl alcohol of 6 (parts by weight), 1% fourth position decalactone of 3 (parts by weight), the 1%3-methylthiopropionaldehyde of 5 (parts by weight) evenly mixes, and prepares allotment type liquid pork essence spline.Draw through subjective appreciation: allotment type liquid pork essence N1 pork gives off a strong fragrance, and has certain sauce fragrance breath, and whole fragrance is natural true to nature, and harmony is better, and voluptuousness is more abundant, and is higher with allotment type liquid pork essence standard specimen N similarity.With allotment type liquid pork essence standard specimen N, allotment type liquid pork essence spline N1 respectively takes by weighing 0.1 gram, place the 10mL sample bottle respectively, the analytical parameters of setting smell signature analyzer FOX4000 (Electronic Nose), wherein: carrier gas is synthetic dry air, flow velocity 150mL/min, the generation time that head space produces parameter is 120s, produce 60 ℃ of temperature, agitation speed 500r/min, the volume injected of head space injection parameters is 500 μ L, injection speed 500 μ L/s, entry needle cumulative volume 2.5mL, 60 ℃ of entry needle temperature, acquisition time getparms are 120s, lag time 600s.By smell signature analyzer FOX4000 (Electronic Nose) described allotment type liquid pork essence standard specimen and described allotment type liquid pork essence spline are carried out analytical test, form with column diagram and radar map is analyzed comparison to the fragrance profile between described allotment type liquid pork essence standard specimen and the allotment type liquid pork essence spline, calculates the similarity of allotment type liquid pork essence spline at last according to formula (I):

$\mathrm{SI}(\%)=(1-\frac{\sqrt{\underset{i=1}{\overset{18}{\mathrm{\Σ}}}{\left(\right|\mathrm{Ai}|-|\mathrm{Bi}\left|\right)}^2}}{\sqrt{\frac{1}{2}\underset{i=1}{\overset{18}{\mathrm{\Σ}}}{\left(\right|\mathrm{Ai}|+|\mathrm{Bi}\left|\right)}^2}})\×100---\left(I\right)$

Ai represents the response signal value of allotment type liquid pork essence standard specimen on i sensor in the formula (I), and Bi represents the response signal value of allotment type liquid pork essence spline on i sensor, and SI represents similarity.

The analysis result of allotment type liquid pork essence spline N1 and allotment type liquid pork essence standard specimen N is analyzed comparison by column diagram and radar map, by Figure 1A, Figure 1B as can be seen, both local flavor profile difference are less, and to calculate similarity SI by the smell signature analyzer be 94.61%, as seen allotment type liquid pork essence spline N1 and allotment type liquid pork essence standard specimen N similarity degree are very high, and the result matches with subjective appreciation.

Embodiment 2, the mensuration of allotment type liquid pork essence spline N2 and allotment type liquid pork essence standard specimen N similarity

1%2-methyl-3-the thiomethylfuran that in beaker, adds 4 (parts by weight) successively, 2% furanone of 10 (parts by weight), 10%3-sulfydryl-2-the butanone of 2 (parts by weight), 10% butyric acid of 2 (parts by weight), 7 (parts by weight) 1%2,3, the 5-trimethylpyrazine, 1% cassia oil of 2 (parts by weight), 1% anisic aldehyde of 4 (parts by weight), 1% pair of (2-methyl-3-furyl) disulfide of 3 (parts by weight), 10%4-methyl-5-the hydroxy ethylthiazole of 7 (parts by weight), 1% ethyl maltol of 15 (parts by weight), 1% difurfuryl dithioether of 7 (parts by weight), 1% furfuryl mercaptan of 4 (parts by weight), the 1%3-methyl mercapto propyl alcohol of 12 (parts by weight), 1% fourth position decalactone of 6 (parts by weight), the 1%3-methylthiopropionaldehyde of 11 (parts by weight) mixes, prepare allotment type liquid pork essence N2, draw through subjective appreciation: allotment type liquid pork essence N2 pork characteristic perfume is outstanding, sauce is aromatic strongly fragrant, voluptuousness is abundant, whole fragrance is natural true to nature, harmony is good, and N is very similar to allotment type liquid pork essence standard specimen.

Allotment type liquid pork essence standard specimen N, allotment type liquid pork essence spline N2 are respectively taken by weighing 0.1 gram, place the 10mL sample bottle respectively, set the Electronic Nose analytical parameters and each essence is carried out analytical test by embodiment 1 identical mode.By Fig. 2 A, Fig. 2 B as can be seen, both local flavor profiles are similar substantially, and to calculate similarity SI by the smell signature analyzer be 98.44%, and as seen allotment type liquid pork essence spline N2 is similar substantially to allotment type liquid pork essence standard specimen N, and the result matches with subjective appreciation.

Embodiment 3, the mensuration of allotment type liquid pork essence spline N3 and allotment type liquid pork essence standard specimen N similarity

1%2-methyl-3-the thiomethylfuran that in beaker, adds 6 (parts by weight) successively, 2% furanone of 30 (parts by weight), 10%3-sulfydryl-2-the butanone of 25 (parts by weight), 10% butyric acid of 25 (parts by weight), 10 (parts by weight) 1%2,3, the 5-trimethylpyrazine, 1% cassia oil of 6 (parts by weight), 1% anisic aldehyde of 6 (parts by weight), 1% pair of (2-methyl-3-furyl) disulfide of 25 (parts by weight), 10%4-methyl-5-the hydroxy ethylthiazole of 10 (parts by weight), 1% ethyl maltol of 35 (parts by weight), 1% difurfuryl dithioether of 10 (parts by weight), 1% furfuryl mercaptan of 25 (parts by weight), the 1%3-methyl mercapto propyl alcohol of 25 (parts by weight), 1% fourth position decalactone of 10 (parts by weight), the 1%3-methylthiopropionaldehyde of 25 (parts by weight) mixes, prepare allotment type liquid pork essence spline N3, draw through subjective appreciation: allotment type liquid pork essence N3 pork characteristic perfume is outstanding inadequately, and sour fragrance is heavier, the chemistry breath is more obvious, whole fragrance is coordinated inadequately, and N differs bigger with the pork essence standard specimen.

Allotment type liquid pork essence standard specimen N, allotment type liquid pork essence N3 are respectively taken by weighing 0.1 gram, place the 10mL sample bottle respectively, set the Electronic Nose analytical parameters and each essence is carried out analytical test by embodiment 1 identical mode.By Fig. 3 A, Fig. 3 B as can be seen, both local flavor profile difference are bigger, and to calculate similarity SI by the smell signature analyzer be 79.79%, and as seen allotment type liquid pork essence N3 and allotment type liquid pork essence standard specimen N similarity degree are lower, and the result matches with subjective appreciation.

Above said content only is the basic explanation of the present invention under conceiving, and according to any equivalent transformation that technical scheme of the present invention is done, all should belong to protection scope of the present invention.

Claims (1)

1. the method for a fast measuring similarity of pork flavor is utilized the similarity of smell signature analyzer FOX4000 fast measuring allotment type liquid pork essence, comprises the following steps:

The analytical parameters of A, setting smell signature analyzer FOX4000, wherein: carrier gas is synthetic dry air, flow velocity 150mL/min, the generation time that head space produces parameter is 120s, 60 ℃ of temperature of generation, agitation speed 500r/min, the volume injected of head space injection parameters is 60 ℃ of 500 μ L, injection speed 500 μ L/s, entry needle cumulative volume 2.5mL, entry needle temperature, and acquisition time getparms is 120s, lag time 600s;

B, allotment type liquid pork essence standard specimen and allotment type liquid pork essence spline are respectively taken by weighing 0.1 gram, place sample bottle respectively, the analytical parameters of A setting carries out analytical test by smell signature analyzer FOX4000 to described allotment type liquid pork essence standard specimen and described allotment type liquid pork essence spline set by step, form with column diagram and radar map is analyzed comparison to the fragrance profile between described allotment type liquid pork essence standard specimen and the allotment type liquid pork essence spline, calculates the similarity of allotment type liquid pork essence spline at last according to formula (I):

$\mathrm{SI}(\%)=(1-\frac{\sqrt{\underset{i=1}{\overset{18}{\mathrm{\Σ}}}{\left(\right|\mathrm{Ai}|-|\mathrm{Bi}\left|\right)}^2}}{\sqrt{\frac{1}{2}\underset{i=1}{\overset{18}{\mathrm{\Σ}}}{\left(\right|\mathrm{Ai}|+|\mathrm{Bi}\left|\right)}^2}})\×100---\left(I\right)$

Ai represents the response signal value of allotment type liquid pork essence standard specimen on i sensor in the formula (I), and Bi represents the response signal value of allotment type liquid pork essence spline on i sensor, and SI represents similarity.

Images